Kent Scientific CODA User manual

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Non-Invasive!Blood!Pressure!System!
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©!2016!Kent!Scientific!Corporation.!Documentation!Version!2.00.02,!For!Software!Version!4.1,!June!16,!2016.!
Windows!is!a!registered!trademark!of!Microsoft!Corporation!in!the!United!States!and!other!countries.!
CODA!is!a!trademark!of!Kent!Scientific!Corporation.!
Table&of&Contents&
!
Part&1:&CODA&System&User’s&Guide&
System!Requirements!–!1!
Included!Equipment!–!2!
The!CODA!Controller!–!3!
Getting!Started!–!4!
Install!the!CODA!Software!–!4!!
Install!the!CODA!System!Hardware!–!5!!
Connect!Your!Animals!to!the!CODA!System!–!6!
Using!the!CODA!Software!–!8!
Terminology!–!8!
User!Interface!–!8!
Setting!Up!and!Running!Experiments!–!9!
While!Your!Experiment!Runs!–!15!
Status!Codes!!–!16!
Monitoring!Experiments!–!17!
Replacing!a!Cuff!Bladder!–!17!
Sample!Displays!–!18!!
Running!More!Sessions!of!an!Existing!Experiment!–!19!
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Part&2:&Software&Reference&
File!Menu!–!20!
File!>!New!>!Experiment!(Experiment!Wizard)!–!20!
File!>!New!>!Session!(Session!Wizard)!–!20!
File!>!Open!(Experiment!Manager)!–!20!
File!>!Exit!–!23!
Tools!Menu!–!24!
Tools!>!Device!Manager!!–!24!!
Tools>!Experiment!Manager!–!26!
Tools!>!User!and!Animal!Manager!!–!26!
Tools!>!Backup/Restore!–!26!
Help!Menu!–!26!
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Part&3:&Theory&of&Operation&
Concepts!of!Non-Invasive!Blood!Pressure:!Tail!Cuff!Method!!–!27!
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Part&4:&General&Information&
Warranty,!Guarantee!and!Contact!Information!–!32!!
System!Specifications!–!33!
Cleaning!and!Decontamination!–!34!
Troubleshooting!–!35!
Quick!Start!Guide!–!36!
Cuff!Testing!Instructions!–!38!
Calibration!Verification!Kit!Instructions!–!39!
Return!Procedure!–!40&

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!
1!
Part&1:&CODA&System&User’s&Guide&
The!CODA™!Non-Invasive!Blood!Pressure!System!monitors!blood!pressure!in!mice,!rats,!gerbils!and!
other!small!animals!with!tails.!65(0'(#.5(7$'01#$78(0#5$#7$7(.%(*+52.%09$7(:.%(+'$(0#(2+;*#(*44,0<*50.#'=(
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CODA!provides!the!highest!degree!of!accuracy,!versatility,!sensitivity!and!reproducibility!for!your!
studies.!Using!the!principle!of!Volume!Pressure!Recording!(VPR),!the!CODA!automatically!performs!
multiple!rapid,!simultaneous!measurements!of!six!physiological!parameters:!systolic!blood!pressure,!
diastolic!blood!pressure,!mean!pressure,!heart!rate,!tail!blood!flow!and!tail!blood!volume.!See!page!27!
for!more!about!types!of!blood!pressure!recording.!!
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32$()$52.7((
An!occlusion!tail!cuff!is!inflated!to!impede!the!blood!flow!to!the!tail.!The!cuff!is!deflated!slowly!and!a!
second!tail!cuff,!incorporating!the!VPR!sensor,!measures!the!physiological!characteristicsof!the!
returning!blood!flow.!As!the!blood!returns!to!the!tail,!the!VPR!sensor!cuff!measures!the!tail!swelling!that!
results!from!arterial!pulsations!from!the!blood!flow.!Systolic!blood!pressure!is!automatically!measured!
at!the!first!appearance!of!tail!swelling.!Diastolic!blood!pressure!is!automatically!measured!when!the!
increasing!rate!of!swelling!ceases!in!the!tail.!
System&Requirements&
To!run!the!CODA!Software,!you!will!need:(!
£ a!computer!running!Windows®!7,!8!or!10—64!bit!with:!
§ 2.0GHz!or!higher!processor!
§ 2GB!RAM!
§ 2!USB!ports!
§ CD-ROM!
§ video!card!at!least!1024!x!768!resolution!
§ internet!connection!
£ a!CODA!Controller!connected!to!the!computer!by!USB!
£ a!mouse!or!touchpad!
£ at!least!one!of!these!CODA!Systems:!
§ CODA!Monitor!!—!!for!one!animal!at!a!time!
§ CODA!High!Throughput!—!!for!2,!4,!6!or!8!animals!at!a!
time!
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Our!email!newsletter!will!keep!you!up!to!date!on!any!
'.:5"*%$(+47*5$'
.!Sign!up!at!
((((
"""=>$#5'<0$#50:0<=<.;!or!contact!Kent!Scientific!at!???@ABC@???B=!
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This!Owner’s!Manual!is!
for!users!running!CODA!
software!and!using!a!
F.7*(/012(32%.+124+5(
F.#5%.,,$%.!

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2!
Included&Equipment&
Your!CODA!System!will!include!different!equipment,!depending!upon!exactly!what!you!have!ordered.!!
You!also!have!the!option!of!ordering!a!laptop!with!pre-installed!CODA!software!from!Kent!Scientific.!!
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CODA!Systems!include:!
£
High!Throughput!CODA!Controller!with!capability!of!up!to!8!Channels—1!
£
Power!supply—1!
£
CODA!Controller!stand—1!
£
Occlusion!Cuff!Kit(s)u—1!kit!of!2!cuffs!per!Channel!
£
VPR!Cuff!Kit(s)u—1!kit!of!at!least!1!cuff!per!Channel!
£
Cuff!replacement!bladdersuu—12!per!cuff!
£
Replacement!O-Rings!or!Caps—1!pair!per!cuff!
£
Rodent!Holdersuuu—1!per!Channel!
£
Animal!Warming!Platformuuuu—1!for!1!to!4!channels,!2!for!5!to!8!Channels!
£
CODA!Cover!–1!for!each!Animal!Warming!Platform!
£
Infrared!Thermometer—1!
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uEach!Cuff!Kit!will!be!sized!for!either!Mouse!or!Rat!according!to!your!order!specification.!
uu See!page!38!for!Cuff!Testing!Instructions.!
uuu Rodent!Holder!size!will!match!your!order!specification.!
uuuuEach!Animal!Warming!Platform!holds!up!to!4!animal!holders.!
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3!
The&CODA&Controller&
These!pictures!show!the!CODA!High!Throughput!Controller.!
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!Power!supply!connector!
!USB!port!for!computer!connection!
!Diagnostic!port!
!Power!On/Off!switch!
!!Occlusion!cuff!port!connectors
!!VPR!cuff!port!connectors!

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4!
Getting&Started&
Four!steps!are!required!to!prepare!your!CODA!System!for!use:!
I5$4(JK!Install!the!CODA!software.!See!instructions!below.!
I5$4(CK!Install!the!CODA!System!hardware.!See!page!5.!
I5$4(LK!Configure!your!experiment!using!the!CODA!software.!See!page!9.!
I5$4(MK!Connect!your!animals!to!the!CODA!System.!See!page!6.!
Before!you!begin!your!experiment,!we!recommend!training!your!animal!
to!use!the!holders!in!three!or!four!fifteen!minute!sessions.!
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Step&1:&Install&the&CODA&Software&
To!install!your!CODA!software:!
1. Close!all!open!applications!on!your!PC.!
2. Insert!the!CODA!software!disk!into!the!CD!drive!of!your!PC.!
3. Navigate!to!the!CD!drive!and!run!setup.exe.!
4. Follow!the!on-screen!directions!to!install!your!CODA!software.!
If!your!PC!does!not!have!a!CD!drive,!please!call!Kent!Scientific.!!
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You!will!need!to!adjust!some!of!your!Regional!and!Language!settings!if!you!are!installing!the!
CODA!software!onto!a!computer!purchased!outside!the!United!States!or!if!you!see!this!or!a!
similar!error!during!installation:!
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1. Open!the!Windows!Control!Panel!and!select!Regional and Language Options.!!
2. At!the!Regional Options!tab,!select!the!language!setting!that!most!closely!matches!
your!location—for!example,!French (France)—and!click!Customize.!
3. In!both!the!Numbers!and!the!Currency!tabs,!set!Decimal Symbol!to!be!a!point!(=).!!
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For!additional!help,!refer!to!the!
Quick!Start!Guide
,!Cuff!Testing!Instructions
!and!Calibration!
Verification!Instructions
!in!the!
Troubleshooting
!section!beginning!on!page!35.!!
Skip!Step!1!if…!
…you!have!purchased!
the!optional!laptop!from!
Kent!along!with!your!
CODA!System.!In!that!
case,!the!CODA!software!
is!already!installed.!!

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5!
Step&2:&Install&the&CODA&System&Hardware&
To!install!your!CODA!hardware:!
1. Choose!an!appropriate!location.!Proper!temperature!is!essential!for!accurate!NIBP!
measurements.!The!room!should!be!at!or!above!26°C.!A!room!that!is!too!cool!will!prevent!an!
animal!from!thermoregulating,!resulting!in!reduced!tail!blood!flow,!which!makes!accurate!
reading!difficult.!Keep!in!mind!that!cold!steel!tables!or!air!conditioning!vents!can!affect!room!
temperature.!!
2. Plug!the!CODA!Controller!into!a!standard!wall!outlet.!
3. Connect!the!Controller!to!a!USB!port!of!your!computer.!
4. Remove!the!caps!from!the!Controller!VPR!ports!you!intend!to!use.!See!page!3.!They!should!!be!
put!back!in!place!when!the!system!is!not!in!use.!
5. Attach!a!VPR!Cuff!to!each!VPR!port.!
6. Repeat!steps!4!and!5!for!the!O-Cuff!port(s).!!
7. Power!the!Controller!on.!
8. Set!up!the!Animal!Warming!Platform!by!plugging!it!into!a!standard!wall!outlet.!This!will!cause!it!
to!come!on!automatically.!!
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Each!Animal!Warming!Platform!accommodates!up!to!four!animals.!Your!CODA!System!may!
include!one!or!two!Warming!Platforms,!depending!upon!the!size!of!your!Controller.!
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Begin!warming!on!all!four!channels!by!pressing!the!START/STOP!button.!Press!it!again!to!stop!
warming.!All!controls!apply!to!all!four!channels;!channels!cannot!be!controlled!independently.!
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The!Platform!will!turn!off!automatically!after!1!hour,!shown!as!1.0!in!the!time!selection!area.!Use!
the!UP!and!DOWN!buttons!on!the!right!side!of!the!
Platform!to!change!the!time!for!automatic!shut-off!in!
increments!of!half!an!hour.!The!available!range!is!0.0!to!
9.5!(zero!to!nine!and!a!half!hours).!Note!that!setting!the!
time!to!0.0!stops!warming!on!all!four!channels.!!
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Three!temperature!levels!are!available:!
§ Level!1!≅!32°C!
§ Level!2!≅!35°C!
§ Level!3!≅!38°C!
D.5$K!These!temperatures!are!approximate!and!depend!
upon!your!operating!environment.!
!&
When!warming!is!active,!
the!green!LED!light!
associated!with!a!
channel!will!flash!when!
that!channel!reaches!
the!set!temperature.!
When!the!temperature!
of!a!channel!falls!below!
the!set!level!by!more!
that!0.5°C,!the!light!
glows!steadily!to!
indicate!that!warming!is!
active!again.!
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6!
Step&3:&Configure&Your&Experiment&
The!CODA!software!will!lead!you!through!the!necessary!steps!to!configure!your!experiments.!See!Setting!
Up!and!Running!Experiments!beginning!on!page!9!for!details.!!
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Step&4:&Connect&Your&Animals&to&the&CODA&System&
Before!you!begin!your!experiment,!we!recommend!training!your!animal!to!use!the!holder!in!three!or!
four!fifteen!minute!sessions.!
1. Set!the!Animal!Warming!Platform!to!level!3!by!pressing!the!UP!
button!to!go!up!from!the!default!level!of!2.!
2. Temporarily!cover!the!Warming!Platform!with!the!CODA!Cover!
to!retain!the!warmth.!
3. Visually!inspect!all!cuff!bladders!and!replace!any!bladder!that!is!
starting!to!yellow!or!crack.(!
4. Place!the!animal!in!the!holder,!if!you!are!using!it:!
0= Remove!the!rear!gate!of!the!animal!holder.!(
00= Slide!the!nose!cone!close!to!the!front!of!the!holder!and!tighten!the!thumb!nut.((
000= Place!the!animal!into!the!holder.!Allow!the!animal!to!enter!the!holder!freely,!using!as!little!
force!as!possible!to!get!the!animal!into!the!holder.((
0R= Make!sure!that!the!tail!of!the!animal!extends!out!of!the!rear!of!the!holder.((
R= Replace!the!rear!gate!on!the!holder!and!tighten!the!thumb!nut.((
R0= Remove!the!Cover!from!the!Animal!Warming!Platform!and!place!the!animal!holder!in!the!V!
groove!on!the!Platform.!(
R00= Adjust!the!nose!cone!so!that!the!animal’s!movement!is!limited!and!the!animal!appears!
comfortable.!Ensure!that!the!animal!cannot!turn!around!inside!the!holder!and!lock!the!
nose!cone!in!place!by!positioning!the!nut!in!the!groove.(
5. Place!the!cuffs!on!the!animal,!following!the!same!procedure!if!you!are!or!are!not!using!a!holder:!
0= Slide!the!Occlusion!Cuff!up!near!to!the!base!of!the!animal’s!tail.!It!should!slide!freely,!but!fit!
closely!when!not!pressurized.!If!you!encounter!resistance!while!sliding!the!cuff!up!the!tail,!
stop!and!do!not!force!the!cuff!further.!Forcing!the!cuff!will!only!compress!the!animal’s!
underlying!arteries!and!veins!resulting!in!poor!or!non-existent!blood!pressure!readings.!
Position!it!as!close!to!the!base!of!the!tail!as!possible!without!force.(
See!pictures!on!next!page.!
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IMPORTANT)
When!you!use!the!holder,!
ensure!that!the!animal’s!!
head!is!not!turned!and!its!
body!is!not!compressed!in!
any!way.!
!!

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7!
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00= Slide!the!VPR!cuff!up!the!tail,!with!the!larger!diameter!end!first,!until!you!reach!the!
Occlusion!Cuff.!If!you!feel!resistance!while!sliding!the!VPR!Cuff!up!the!tail,!do!not!force!it!
into!position.!Simply!use!a!cuff!one!size!larger.!Tail!swelling!can!be!sensed!on!any!part!of!
the!tail!as!long!as!there!is!a!sufficient!portion!of!the!tail!in!the!VPR!Cuff.(
!!!!!!!!!!!!!!!!!!!!!!!!!! !!!!!!! !
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iii. Secure!the!Occlusion!Cuff!tubing!inside!the!groove!located!in!back!of!the!black!fastener!on!
top!of!the!holder.!This!will!assist!in!keeping!the!cuff!in!position.!To!maintain!the!position!of!
the!VPR!Cuff,!secure!the!VPR!Cuff!tubing!inside!the!groove!located!in!back!of!the!black!
fastener!on!top!of!the!holder.!!!
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Before!you!start!measurements,!lift!the!CODA!cover!and!measure!the!animal’s!tail!temperature!by!
pointing!the!infrared!thermometer!at!the!tail’s!base.!The!temperature!should!be!between!32!and!35°C.!
Do!not!start!the!experiment!If!the!temperature!is!not!at!least!32°C.&Make!sure!the!entire!length!of!the!
animal’s!tail!rests!on!the!warming!surface.!You!can!use!the!CODA!Cover!over!the!tail!to!help!maintain!
optimal!temperature!for!measuring!blood!pressure.!
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See!Replacing!a!Cuff!Bladder!on!page!17!for!information!about!changing!a!
leaking!bladder.!!
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8!
Using&the&CODA&Software&
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The!CODA!Software!uses!these!terms:!
£ User!–!any!person!involved!in!CODA!experiments,!such!as!a!researcher!or!scientist!
£ Key User!–!the!main!person!associated!with!a!given!experiment!
£ Animal –!any!animal!associated!with!experiments!
£ Animal!Type –!the!sensitivity!level!of!an!animal,!either!Mouse!(higher)!or!Rat!(lower)!
£ Animal Group!–!a!group!of!individual!Animals!assigned!to!a!User!
£ Cycle!–a!single!performance!of!a!measurement!!
£ Set!!–!a!group!of!Cycles!in!the!same!Experiment!
£ Session — one!or!more!Sets!of!Cycles!in!a!single!Experiment!
£ Experiment!!–!!a!collection!of!Sessions!
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O'$%(6#5$%:*<$(
The!general!CODA!user!interface!will!be!familiar!to!Windows!users.!You!will!see!the!usual!menus,!icons,!
drop-down!lists,!dialog!boxes,!etc.!CODA!uses!a!variety!of!screen!styles!and!input!fields.!For!example,!In!
some!case,!you!choose!information!from!a!drop-down!box.!In!others,!you!type!directly!into!an!entry!
field.!The!User!and!Animal!Manager!uses!a!slightly!different!form!for!input:!!
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£ 3.(*77(*(#$"($#5%S:!Click! ,!then!type!in!the!pop-up!box.!!
£ 3.(7$,$5$(*#($#5%S:!Click!on!an!entry,!then!click! .!!!
£ 3.($705(*#($#5%S:!Click!the!entry!twice!(do!not!double-click)!and!type!the!new!information.!
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P%%."(T+55.#'(
Some!screens!contain! !and! !buttons.!!
Use!these!buttons!to!select!items!from!a!list!by!click!an!item!from!the!list!on!the!left!and!clicking! !to!
add!to!the!list!on!the!right.!!
You!can!add!items!individually,!or!use Shift-click!or Ctrl-click!to!highlight!several!items!and!add!them!all!
with!one! !click.!To!remove!items!from!the!list,!highlight!them!in!the!right-side!box!and!click! .!
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9!
Setting&Up&and&Running&Experiments&
To!run!an!experiment,!you!must!go!through!this!software!sequence:!
J= Select!the!CODA!device(s)!to!use.&
C= Create!or!add!to!your!lists!of!Users,!if!necessary.
L= Create!the!Experiment!and!run!a!Session!of!that!Experiment.((Once!you!have!created!an!
experiment,!you!can!return!to!it!later!to!run!new!sessions.!
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J= Select&CODA&Devices(
When!you!open!the!CODA!Software,!the!Device!Manager!appears.!Click!Use All Displayed Devices!to!
proceed!using!the!detected!CODA!device(s).!If!you!do!not!want!to!use!all!the!detected!devices,!highlight!
those!you!do!not!want!to!use!and!click!Remove.!To!change!your!selections!while!you!are!running!the!
CODA!software,!simply!open!the!Device!Manager!and!modify!your!choices.!
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To!open!the!Device!Manager:!!
Tools > Device Manager!!
or!
!!
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For!more!information!about!using!the!Device!Manager,!see!page!24.!
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C= Create&a&List&of&Users&and&Animals&
You!must!create!a!database!of!Users,!Animals!and!Animal Groups!for!your!Experiments.!You!will!be!selecting!
items!from!these!categories!for!each!individual!experiment!you!create.!Create!your!database!with!the!
User!and!Animal!Manager.!You!may!enter!the!information!for!your!upcoming!Experiment only!or!
information!that!you!plan!to!use!in!future!experiments!as!well.!
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Running&Multiple&Experiments&&
You!may!create!as!many!Experiments!as!you’d!like!and!open!Sessions!for!them!whenever!
you!need!to.!However,!only!one!Experiment!at!a!time!may!be!running!!on!a!given!
Controller!and!in!a!given!instance!of!the!CODA!software.!Open!multiple!instances!of!the!
CODA!Software!to!run!multiple!Experiment!sessions!simultaneously,!assigning!the!
appropriate!CODA!Controllers!to!each!instance!of!the!software.!See!page!24(
for!more.!
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Important!!Click
Save!or!Save & Close!after!any!addition,!edit!or!deletion!to!save!your!changes.!

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!
10!
To!open!the!User!and!Animal!Manager:!!!
Tools > User and Animal Manager
or!
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There!are!three!tabs!in!the!User!and!Animal!Manager:!!
£ O'$%'K((Enter!the!names!of!any!Users!for!the!Experiment!you!
will!create.!!
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£ P#0;*,'K(!Enter!the!Animals!for!your!Experiment!and!assign!them!to!an!Animal!Type,!which!is!a!
sensitivity!level,!either!Mouse!or!Rat.!!
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P5(P,,(3*U'K(
•Click! ,!then!type!to!
add.!
•Click!an!entry,!then!
!to!delete.!
•Click!an!entry!twice!
and!type!to!edit.!

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11!
£ P#0;*,(V%.+4'K((Create!one!or!more!groupings!of!Animals!to!accommodate!the!way!your!Users!
are!associated!with!Animals for Experiments.!Specify!a!name!for!an!Animal Group!and!any!
descriptive!information!you'd!like.!A!dropdown!box!lets!you!assign!a!User!to!the!group.!Click!OK!
when!you!are!finished.!Make!sure!that!the!group!you!just!created!is!highlighted!before!you!
make!any!selections!from!the!list.!!
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The!CODA!software!requires!that!you!create!at!least!one!Animal Group.!You!can!put!all!your!
Animals!into!a!single!group,!or!you!can!break!your!animals!into!groupings!to!be!associated!with!
individual!Users.!An!individual!Animal!can!be!assigned!to!more!than!one!group!if!multiple!Users
will!be!working!with!some!of!the!same!animals.!
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L= Create&and&Run&an&Experiment&&&
Before!you!can!run!an!Experiment,!you!must!create!it,!giving!it!a!name!and!assigning!O'$%'!and!P#0;*,'!
to!it.!Once!you!have!created!an!Experiment,!you!can!return!to!it!at!any!time!for!additional!sessions.!
! !
PU.+5(W$,$50#1(O'$%'(.%(P#0;*,'!
A!deleted!entry!that!has!never!been!part!of!any!CODA!experiment!is!removed!from!the!
database.!!A!deleted!entry!that!has!been!part!of!an!experiment!is!marked!inactive!but!
remains!in!the!database!so!it!will!be!available!to!the!CODA!software!for!generating!reports.!
You!can!reactivate!a!deleted!entry!by!adding!it!again!with!the!same!name.!

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12!
To!begin!creating!a!new!Experiment,!open!the!Experiment Wizard:!!
File > New > Experiment
or!
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The!Experiment!Wizard!is!a!series!of!four!screens.!To!create!an!
Experiment!successfully!and!save!it,!you!must!enter!the!required!
information!on!all!four!screens!and!start!the!Experiment.!If!you!do!not!
end!the!Experiment!Wizard!by!pressing!Start!to!begin!a!running!your!
Experiment,!your!information!will!not!be!saved.!!
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At!Screen!1!of!the!Experiment!Wizard:!
£ Name!your!Experiment.!
Type!any!combination!of!up!to!50!letters,!numbers,!symbols!and!spaces!in!the Experiment Name!
field.!
!
£ Assign!a!Key User.!
Choose!the!main!person!to!be!associated!with!the!Experiment from!the!dropdown!list!in!the Key
User!field.!If!the!User!you!need!is!not!in!the!list,!click!Manage Users!to!open!the!Users!and!Animals!
Manager!and!add!the!name!you!need.!
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£ Associate!any!other!personnel!with!the!Experiment.!!
Select!names!from!the!shaded!box!under Additional Users!and!click! !to!add!them!to!the!list.!!
When!you!are!finished,!click!Next!for!the!second!screen!of!the!Experiment!Wizard.!
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If!the!New!choice!of!the!
File!menu!is!grayed,!
check!that!your!CODA!
device!is!connected,!
turned!on!and!
communicating.!See!
page!24(for!more.!
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13!
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At!Screen!2!of!the!Experiment!Wizard:!
£ Name!your!Session.!
Type!any!combination!of!up!to!50!letters,!numbers,!symbols!and!spaces!in!the Session Name!field.!
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£ Specify!details!about!the!Sets!and!Cycles!for!the!Session.!
A!Session!is!made!up!of!a!number!of!Sets!of!Cycles=!Indicate!how!many!Sets!make!up!the!Session,!
how!long!to!pause!between!Sets,!how!many!Cycles!make!up!each!Set,!how!long!to!pause!
between!Cycle,!and!how!many!Acclimation Cycles!to!run!at!the!beginning!of!the!Session.!Defaults:!
1!Set/Session,!30sec!between!Sets,!10!Cycles/Set,!5sec!between!Cycles,!5!Acclimation!Cycles!
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£ Specify!Deflation Time!for!the!cuffs.!
Choose!the!number!of!seconds!of!deflation!during!which!measurements!will!be!made.!Default:!
15sec.!!
!
£ Specify!)0#0;+;(Y.,+;$=(
)0#0;+;(Y.,+;$(0'!the!smallest!volume!of!blood!that!will!cause!a!blood!pressure!reading!to!be!
made.!If!a!channel!registers!under!this!amount,!no!reading!will!be!made!at!that!channel.!
Default:!15ul!
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£ Specify!W0'4,*S(I5S,$=((
Select!how!the!data!from!this!Session!will!be!presented!in!the!display!that!appears!while!your!
Experiment!is!running.!Choose!a!single!graph!for!each!device,!a!graph!for!each!channel!you!are!
using!or!a!text-only!display.!
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When!you!are!finished,!Click!Next!for!the!third!screen!of!the!Experiment!Wizard.!!!!
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14!
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At!Screen!3!of!the!Experiment!Wizard:!
£ Assign!individual!Animals!to!CODA!channels.!
Select!Animals!and!click!! !to!add!them!to!the!next!open!channel.!!
!
You!can!choose!Animals!from!one!or!more!groups.!To!use!Animals,!either!in!addition!to!or!instead!
of!those!currently!listed!on!the!left!side!of!the!screen,!you!will!need!to!display!the!Animals!from!
one!or!more!additional!Animal Groups.!Begin!by!choosing!a!User!from!the!Select User!dropdown!
list.!Then!choose!a!group!associated!with!that!User.!A!list!of!any!unassigned!Animals!in!that!group!
will!appear.!Select!and!assign!individual!Animals!as!above.!
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If!the!Animals!you!need!are!not!in!any!of!your!Animal!Groups,!click!Manage!Animals!to!open!the!User!
and!Animal!Manager!and!add!them.!!!
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When!you!are!finished,!click!Next!for!the!fourth!screen!of!the!Experiment!Wizard.!!
( (
The!available!CODA!channels!are!those!you!have!selected!with!the!
Device Manager
.!The!
CODA!software!sequentially!numbers!the!channels!of!all!the!devices!you!have!said!you!
plan!to!use.!You!can!assign!only!one!Specimen!to!a!single!channel,!and!you!must!assign!
them!in!channel!order.!

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15!
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At!Screen!4!of!the!Experiment!Wizard:!
£ Start!the!Experiment.!!
§ Ensure!that!all!your!Animals!are!in!place.!See!Connect!Your!Animal!to!the!CODA!System!on!
page!6.!!
§ Cap!off!any!unused!channels.!
§ Click!Start!to!start!making!measurements!and!collecting!data.!!
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While&Your&Experiment&Runs&
Once!your!Experiment!begins!running,!the!screen!will!display!real-time!data!about!your!measurements.!
This!can!either!be!in!the!form!of!a!graph!or!text,!depending!upon!how!you!have!set!up!your!Session.!!
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Along!the!top!of!the!screen!you!will!see:!
£ buttons!for!controlling!the!Cycle—Resume!and!Pause!on!the!left,!Stop and Close!on!the!right!!!
£ a!Show Log/Hide Log!button!
£ information!about!the!progress!of!your!Cycle!
£ a!Status Descriptions!button!that!shows!the!meaning!of!the!Status!messages!at!the!bottom!of!the!
screen! !
IMPORTANT!)))If!you!do!not!press!
Start
!at!this!screen,!your!setup!data!will!be!lost.!You!will!
have!to!create!this!Experiment!again!when!you!are!ready!to!run!it.!

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16!
The!left!side!of!the!screen!displays!the!Run Log,!if!you!have!turned!it!on.!For!each!Cycle,!it!shows!you:!
£ the!type!of!Cycle—Acclimation Cycle!or!part!of!a!Set!
£ the!blood!pressure!for!each!Animal!
£ a!validity!code,!either!OK!or!an!error!code!!
£ the!rate!and!volume!of!the!pressure!reading!(meaningful!only!if!the!validity!code!is!OK)!
The!current!Cycle!is!highlighted.!
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I5*5+'(F.7$'(
F.7$(
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G.''0U,$(I.,+50.#(
FAIL_TAIL_VOLUME(
Tail!volume!is!less!than!the!
minimum!acceptable!value!of!
15ul.!
Raise!tail!temperature!to!32°C!to!35°C!by!
increasing!the!warming!level!and!covering!the!tail.!!
TOO_SHORT(
The!time!between!the!diastolic!
and!the!systolic!is!too!short.!
Ensure!animal!is!snug!in!the!holder!and!does!not!
have!room!to!move.!
Raise!tail!temperature!to!32°C!to!35°C!by!
increasing!the!warming!level!and!covering!the!tail.!
TOO_LONG(
The!time!between!the!diastolic!
and!the!systolic!is!too!long!to!
permit!more!than!one!set!on!the!
graph.!
Ensure!animal!is!snug!in!the!holder!and!does!not!
have!room!to!move.!
BAD_RANGE
The!difference!between!the!
systolic!and!the!diastolic!is!less!
than!5.!
Ensure!that!the!equipment!is!in!a!quiet!area,!away!
from!any!causes!of!white!noise!or!vibrations—for!
example,!vibrating!electrical!equipment,!an!air!
vent!or!a!refrigeration!unit.!
WRONG_SHAPE
The!diastolic!value!is!lower!than!
the!systolic.!
This!can!occur!from!any!of!the!causes!discussed!
above!for!the!other!Error!Codes.!
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Each!pressure!trace!is!marked!with!the!points!at!which!systolic!and!diastolic!pressures!are!measured.!
The!O-Cuff!pressure!trace!is!displayed!for!reference.!
!
If!you!have!selected!a!graph!for!each!channel8!the!graphs!will!be!sized!to!allow!all!of!them!to!fit!on!the!
screen!at!the!same!time.!If!you!have!chosen!to!display!one!graph!for!all!channels!on!the!device,!a!legend!
will!identify!the!association!between!trace!color!and!channel.!!
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-.%(52$(3$X5(W0'4,*S(
Bright!colors!and!large!type!are!used!to!make!the!display!readable!from!a!distance.!Current!pressure,!
pulse,!volume!and!flow!appear!along!with!the!pressure!and!status!from!the!previous!Cycle.!In!addition,!
directly!under!the!current!pressure,!one!of!these!messages!appears:! !

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17!
• Prep—preparing!for!a!test!
• Acclimations—this!is!an!Acclimation!Cycle!
• OK—validation!status;!can!also!be!one!of!several!Error!Codes.!See!page!16.!
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After!the!Session!is!complete,!a!report!appears!containing!the!details!of!each Cycle!of!the!Session.!You!
can!print!the!report!and!export!it!to!Excel.!See!page!21!for!information!about!this!Simple Report.!
Monitoring&Experiments&
The!graphical!displays!provide!a!valuable!method!for!monitoring!
your!Experiments!for!potential!problems.!!
• If!the!blue!line!drops!sharply!instead!of!gradually!at!the!
beginning!of!the!cycle,!you!probably!have!a!leak!in!the!VPR!
Cuff!bladder.!!
• If!the!red!line!drops!precipitously,!you!probably!have!a!leak!in!the!O-Cuff.!!
!
If!you!suspect!a!leak:!
• Pause!the!experiment,!change!the!bladder!in!the!cuff!with!the!suspected!leak!and!continue.!!
• If!the!problem!persists,!pause!the!experiment,!remove!the!cuff,!place!the!white!cap!on!the!
channel!and!continue.!Then:!
§ If!the!display!line!returns!to!normal,!you!need!a!new!cuff.!!
§ If!the!problem!persists!with!the!cap!on,!the!leak!is!internal.!In!that!case,!please!contact!Kent!
Scientific!immediately!for!customer!service.!!
For!more!information,!see!Cuff!Testing!Instructions!on!page!38.!!
H$4,*<0#1(*(F+::(T,*77$%(
Bladders!in!both!the!O-Cuffs!and!VPR!Cuffs!will!require!periodic!replacement.!Replacement!bladders!are!
included!with!your!CODA!System!and!more!are!available!by!request.!O'$(.#,S(:%$'2(Z$#5(I<0$#50:0<(4%$@
;$*'+%$7(<+::(U,*77$%(%$4,*<$;$#5'=(!52$%(;*5$%0*,'(.%(%$4,*<$;$#5(U,*77$%'(<.+,7('$%0.+',S(*::$<5(
U,..7(4%$''+%$(%$*70#1'=(!
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1. Remove!the!external!rubber!washers!(O-rings)!that!hold!the!bladder!on!the!cuff!at!each!end.!
2. Slide!the!old!bladder!out!through!either!end!of!the!cuff!and!discard.!
3. Insert!the!new!bladder!inside!the!cuff.!
4. Fold!the!new!bladder!over!each!end!of!the!cuff.!
5. Secure!the!bladder!to!the!cuff!by!sliding!the!O-ring!over!the!ends!of!the!cuff!toward!the!middle!
of!the!cuff.!Be!careful!not!to!over!stretch!the!bladder!material.!
!
The!cuff!bladder!in!the!occlusion!cuff!should!be!wrinkle-free.!Ideally,!the!bladder!in!the!VPR!cuff!should!
be!loose!enough!to!not!hamper!the!expansion!of!the!tail!yet!still!allow!maximum!contact!with!the!
animal’s!tail.!Properly!installed!cuff!bladders!ensure!accurate!and!consistent!blood!pressure!
measurements.
See!Sample!Displays!on!
page!18.!

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18!
Sample Displays& &
YGH(F+::(N$*>'!!
Sample!display!showing!a!leaking!VPR!cuff.!The!top!
channel!shows!a!nearly!horizontal!blue!line,!
indicating!no!leak!in!the!VPR!cuff.!The!bottom!
channel!shows!a!sharp!drop!at!the!beginning!of!the!
cycle,!indicating!a!probable!VPR!cuff!leak.!Notice!the!
red!line!for!the!O-Cuff!drops!smoothly!indicating!no!
leak!in!the!O-Cuff.!!
!@F+::(N$*>'!!
Sample!display!for!O-Cuff!leak.!For!both!channels,!the!red!
line!drops!precipitously!at!the!beginning!of!the!cycle!
indicating!probable!O-Cuff!leaks.!Notice!that!the!blue!line!
for!the!VPR!cuff!remains!close!to!horizontal!indicating!no!
leak!in!the!VPR!cuff.!
!@F+::(3$'5'(
S
how
ing!Good!O-Cuff!!!!!!!!!!!!!!!!!!!!!Showing!O-Cuff!Leak(
!