Kent Scientific MouseSTAT Jr User manual

MouseSTAT® Jr.
Pulse Oximeter for Mice and Rats
Owner’s Manual and
Set-Up Guide

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MouseSTAT Jr.
Pulse Oximeter for Mice and Rats
User Manual and Set-Up Guide
©2015 Kent Scientific Corporation, Documentation Version 1.00.05 12/3/15

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Table of Contents
Part 1: MouseSTAT Jr. User's Guide –3
Welcome to MouseSTAT Jr. –3
Included Equipment –3
Part 2: Getting Started –4
System Assembly –4
Instructions for Use –4
FAQ and Troubleshooting –5
Care and Maintenance –5
Part 3: Theory of Operation –6
MouseSTAT Jr. and Pulse Oximetry –6
Part 4: General Information –8
Contact Information –8
Disclaimer –8
Product Warranty and Satisfaction Guarantee –9
This product is not designed, intended or authorized for use in human applications.

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Part 1: MouseSTAT Jr. User’s Guide
The MouseSTAT Jr. measures oxygen saturation (SpO2) and heart rate up to 900bpm. It easily
integrates into your anesthesia set up, and its small footprint fits in the palm of your hand. It
includes a two illuminated displays that allow you to quickly view vital signs while focusing on
your animal. Visual alert indicators confirm sensor placement, allowing you to identify and
maintain accuracy at the sensor site.
Included Equipment
MouseSTAT Jr. Controller and
power supply
Sensor Options
Clip Sensors
Mouse paw pulse oximeter sensor
Rat paw pulse oximeter sensor
Large animal sensor
Annular Sensors
Pup, paw, and whole body sensor

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Part 2: Getting Started
System Assembly
1. Connect the power supply to the Power socket on the back of the controller.
2. Plug the pulse oximeter sensor into the MouseSTAT Jr. adapter cable.
3. Plug the sensor adapter cable into the Sensor socket.
a. Ensure that the red light is visible in the sensor, and the “Check Connection” indicator
on the MouseSTAT Jr. Controller is not illuminated.
Instructions for Use
1. Turn on the MouseSTAT Jr. by pressing the On/Off button on the back of the controller.
2. Set the minimum heart rate.
a. Press and hold the On/Off button for 5 seconds. The top display will read “Hr”, and the
bottom display will show a numeric value. The value represents the minimum allowed
heart rate that the system will detect.
b. Change the value by pressing the On/Off button.
Minimum Heart Rate Recommendations
Mouse
240
Rat
120
Custom
40-500
c. After 5 seconds of inactivity, the setting will be saved.
3. Place the sensor over the hind paw of the animal so that the red light is centered over the pad
of the paw.
4. When finished, press the Power button to turn off the MouseSTAT Jr.

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FAQ and Troubleshooting
This chart shows common problems you might experience with the MouseSTAT Jr. If none of the
suggested solutions solve your problem, please contact Kent Scientific for assistance.
Problem
Possible Cause
Solutions
Check Connection Light
or
no red light in sensor
Sensor not fully
connected
Ensure that all connections are tight,
especially at the back of the
controller.
Sensor or interface
cable has damaged
wire
Replace the damaged cable
Check Placement Light
or
Inconsistent readings
or
No readings
Incorrect sensor
placement
Reposition sensor on animal's paw
Poor animal
circulation
Investigate type and dose of
anesthetic and other drugs; reduce if
possible
Increase body temperature of animal
to encourage circulation
Care and Maintenance
MouseSTAT Jr. Controller Maintenance
1. Always position the Controller away from areas of potential contamination. Should the unit
become soiled, gently spot clean with a damp wipe or towel. Never use liquids directly on
the Controller.
MouseSTAT Jr. Sensor Maintenance
1. MouseSTAT Jr. sensors are designed to be gentle on small paws. Avoid any practice that
creates tension or strain on the sensor cable or its connections.
2. When not in use, gently coil the MouseSTAT Jr. sensor and store in its package to prevent
tangles or bends in the sensor wire.
3. Gently wipe the sensor clean between uses.

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Part 3: Theory of Operation
MouseSTAT Jr. and Pulse Oximetry
A pulse oximeter measures how much oxygen is carried in the blood. Oxygen saturation and pulse rate
are determined by passing two wavelengths of low intensity light, one red and one infrared, through the
body tissue to a photodetector. The signal strength of each light source depends on the color and
thickness of the body tissue, sensor placement, and the absorption of the arterial and venous blood in
the body tissue.
Oximetry processes the signals, separating out the time-invariant parameters (arterial volume and
SpO2) to identify the pulses and calculate the oxygen saturation. Simple calculations for oxygen
saturation can be performed because blood saturated with oxygen predictably absorbs less red light
than oxygen-depleted blood.
Since measurements of SpO2 depend on a pulsating vascular bed, any condition that restricts blood
flow, such as the use of spring-loaded Y-clip sensors, may make accurate SpO2 and pulse rate readings
impossible.
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MouseSTAT Jr. Sensors
There are two styles of MouseSTAT Sensors: clip and annular:
The mouse and rat clip sensor is used on the paw and is designed to make contact with the
skin without affecting circulation. The clip hinge is not spring loaded, thus reducing the
possibility of ischemia at the sensor site, and detects signals equally well on the front or rear
paw. The large animal sensor is ideal for placement on the tongue, Achilles tendon, ear, toe
webbing, or thigh. 
The annular sensor is used for pups and allows for accurate measurements without making
full contact with the skin. MouseSTAT sensors are available in four sizes: 5mm, 9mm and
13mm. The size refers to the internal diameter of the hole in the center of the sensor. The
MouseSTAT annular sensor does not require contact with the skin to obtain accurate
measurements and is used as a whole-body sensor in neonatal rodents. 
1
Wright, B., Hellyer, P.W., Respiratory Monitoring During Anesthesia: Pulse Oximetry and Capnography.
The Compendium. 1996;18:10

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Sensor Placement
For best results, position the sensor in an area where there the pigment is lightest, such as the palm
(metacarpal or metatarsal) region of the paw.
Clip Sensor
We recommend placing the MouseSTAT clip sensor for pulse oximetry measurements on the metacarpal
or metatarsal section of the paw of adult rodents. The hind paw is optimal because less movement
associated with breathing occurs there compared to the front paw. Depending on the size of the
animal, the large animal sensor is ideal for placement on the tongue, Achilles tendon, ear, toe webbing,
or thigh.
Whole Body (Neonatal Rodents): Annular Sensor
We recommend the annular sensor in neonatal rodents for pulse oximetry measurements around the
body. Lay a portion of the pup's body through the sensor. Full skin contact is not required for
measurements with the annular sensor.
Tail: Considerations Regarding Placement
We do not recommend the MouseSTAT annular sensor for pulse oximetry measurements on the tail.
Significant research over the years has shown the tail of a rat or mouse to be a specialized organ under
control of the CNS for the thermoregulatory control of body temperature. Warming of the subject to the
point of eliciting a thermoregulatory response is required to increase blood flow through the tail and
assure the most accurate SpO2 readings. Experiments of Lemons and Wu
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have shown that:
perfusion in the tail tip is more than tenfold higher than that in the tail base
perfusion in the middle region of the tail increases eightfold during warming compared to
threefold to fourfold in the base and tip.
Our experience shows that pulse oximetry sensors placed simultaneously on a limb and the tail of non-
warmed subjects such as rat pups and mice produce significant differences in signal quality. The limb,
which is a smaller appendage, shows a significant pulse signal quite usable for SpO2 detection, but the
signal from the base tail is almost devoid of pulses and shows mainly a strong respiration artifact.
2
Wu Y, Jiji LM, Lemons DE, Weinbaum S., Department of Mechanical Engineering, City College of the
City University of New York, New York, USA. A non-uniform three-dimensional perfusion model of rat
tail heat transfer. Phys Med Biol. 1995 May;40(5):789-806.

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Part 4: General Information
Thank you for purchasing the MouseSTAT Jr. We truly appreciate your business. We strongly advise that you read
and study this Owner’s Manual to appreciate fully all the features, benefits, and capabilities of the MouseSTAT Jr.
Contact Information
Kent Scientific Corporation Toll-Free: 888-5RATTUS (888-572-8887)
1116 Litchfield Street Outside US: 860-626-1172
Torrington, Connecticut 06790 Fax: [phone]
E-mail: [e-mail] Internet: www.kentscientific.com
Disclaimer
Kent Scientific Corporation makes no representations or warranties, expressed, statutory or implied, regarding the
fitness or merchantability of the components of this system for any particular purpose. Further, Kent Scientific
Corporation is not liable for any damages, including but not limited to, lost profits, lost savings, or other incidental
or consequential damages arising from ownership or use of these products, or for any delay in the performance of
its obligations under the warranty due to causes beyond its control. In no case shall Kent Scientific Corporation's
financial obligation extend beyond the cost of or replacement of the Kent product in question. Kent Scientific
Corporation also reserves the right to make any improvements or modifications to these products described in this
manual at any time, without notice of these changes. All brand and product names used in this manual are the
trademarks of their respective owners.
This product is not designed, intended or authorized for use in human applications.
Product Warranty
The MouseSTAT Jr. has a one (1) year warranty including all parts and labor charges. This warranty does not cover
damage by any cause including, but not limited to, any malfunction, defect or failure caused by or resulting from
unauthorized service or parts, improper maintenance, operation contrary to furnished instructions, shipping or
transit accidents, modifications or repair by the user, harsh environments, misuse, neglect, abuse, accident,
incorrect line voltage, fire, flood, other natural disasters, or normal wear and tear. Changes or modifications not
approved by Kent Scientific Corporation could void the warranty. The foregoing is in lieu of all other expressed
warranties. Kent Scientific Corporation does not assume or authorize any party to assume for it any other
obligation or liability.
Satisfaction Guarantee
Should you experience difficulty with the MouseSTAT Jr., our Technical Support Group will assist you in trouble-
shooting and determining if the product needs to be returned to our facility. We will issue you a Return
Manufacturer Authorization (RMA) number before the product is shipped back for repair. It is at the discretion of
the manufacturer to replace or repair a defective part or product. Please call Customer Service at 888-572-8887 to
obtain a Return Manufacturer Authorization number. Shipments without a RMA number will not be accepted.
Please note that after our 30-day return policy period ends, we will be happy to assist you with your application,
but cannot issue any credit or refund for a returned MouseSTAT Jr.
Prior to shipment, please clean and decontaminate the product of any chemical, biological, or isotopic
contamination. Please include a completed Product Return Form with the shipment. The form can be found on
page 9 of this Owner's Manual. For additional copies, call Kent Scientific Customer Service at 888-572-8887.

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Product Return Form - Complete Steps 1 through 4

1116 Litchfield Street
Torrington, CT 06790
www.kentscientific.com
Tel: [phone]
Toll-free: 888-572-8887
sales@kentscientific.com