Kent Scientific SomnoSuite User manual

SomnoSuite®
Low Flow Anesthesia System
for Mice and Rats
User’s Guide

©2019 Kent Scientific Corporation, Documentation Version 1.02 for On-Unit Help Version 4.02
and Firmware Revision 2.0.1 - August 2019
SomnoSuite®
Anesthesia System
User’s Guide
Technical Help: 860-626-1172

TABLE OF CONTENTS
Introduction
System Components ....................................................................1
Navigating SomnoSuite.............................................................. 2
SomnoSuite®
Small Animal Anesthesia................................................................... 3
Set Up Hardware .......................................................................... 3
Initial Setup ................................................................................... 8
Procedure for Use ....................................................................... 8
Troubleshooting ..........................................................................12
System Customization
Custom Run Screen ....................................................................13
Alarms............................................................................................15
RoVent®
Set Up Hardware .........................................................................17
Initial Setup ................................................................................. 19
Procedure for Use..................................................................... 20
Raw Override Mode...................................................................24
Troubleshooting .........................................................................25
MouseSTAT®
Set Up Hardware ........................................................................28
Initial Setup .................................................................................28
Procedure for Use......................................................................29
Troubleshooting ........................................................................ 30
MouseSTAT Sensor Maintenance .......................................... 30
TOC Continued ...

CapnoScan®
Set Up Hardware .........................................................................31
Initial Setup .................................................................................32
Procedure for Use......................................................................33
Troubleshooting .........................................................................34
CapnoScan Sensor Maintenance............................................34
RightTemp®
Set Up Hardware ........................................................................35
Initial Setup .................................................................................36
Procedure for Use......................................................................37
Troubleshooting .........................................................................38
RightTemp Pad and Sensor Maintenance ............................38
Data Management
PC Connections ..........................................................................39
History - Internal Storage ...................................................... 40
Analog .........................................................................................42
General Information
Contact ........................................................................................43
Warranty .....................................................................................43
Satisfaction Guarantee ............................................................ 44
Specifications ............................................................................ 44
Recommended Flow Rates............................................ 47
Index ...................................................................................48

1
Introduction
The SomnoSuite is not designed, intended, or authorized for use in human applications.
System Components
Front Panel
Back Panel

2
Navigating SomnoSuite®
Module Screens
The SomnoSuite Run screen has a designated screen for each installed module. Touch
the tabs along the top of the screen to view the parameters and important settings for
each module. Touch the tab again to open the module’s extended Setup Menu.
SomnoSuite Menu oers quick access to most features and settings. Touch the Menu
button on the top right corner of the Run Screen.
Anest Setup Menu
(Available menu options may dier based on module configuration.)
Menu
(Screen buttons may vary based on the modules installed.)
Touch Screen

3
SomnoSuite®Small Animal Anesthesia
Set Up Hardware
Scavenging:
Connect a charcoal canister to the Exhaust port.
Air Source:
Using Room Air (internal pump):
Remove the red cap from the Air Inlet Port.
No additional hardware setup is necessary.
Using a Compressed Gas Source:
Connect the Compressed Gas tubing directly to the Compressed Gas port.
• Set gas pressure to 10-15 PSI .
Rear Overlay
Tip: If your gas source does not regulate to this range, connect
a Kent Scientic Pressure Reducer between the gas source and
the SomnoSuite.

4
Diagram showing Pressure Reducer connection:
Diagram showing Pressure Reducer connection
Tubing Congurations:
Connecting an Induction Chamber and a Nose Cone
Depending on your specific module configuration, your SomnoSuite includes a set
of 2-Accessory Connectors or 3-Accessory Connectors. These are used to divide the
SomnoSuite’s airflow to multiple stations or animals.
1. Connect:
1) The white-labeled Accessory Connector to the inspiratory port (INSP).
2) The black-labeled Accessory Connector to the expiratory port (EXP).
2. Designate one branch from each of the connectors to each accessory.

5
SomnoSuite with 2-Accessory Connector
for Chamber and Nose Cone
SomnoSuite ports and accessories are color-coded for
convenience.
White Ports and Connectors Inlet and Inspiratory Line
Black Ports and Connectors Exhaust and Expiratory Line
Blue Clips Branch 1
White Clips Branch 2
Yellow Clips* Branch 3
Setup Examples
*3-Accessory Connectors only.

6
SomnoSuite with 3-Accessory Connector for Chamber and two Nose Cones
SomnoSuite with 3-Accessory Connector for Chamber, Intubation Stand, and RoVent

7
SomnoSuite with External Ventilator
SomnoSuite with Stereotaxic Mask
Please see recommended flow rate
chart on page 47

8
The SomnoSuite Run Screen
Initial Set-Up
The first time the SomnoSuite is used, or any time the accessories have changed, be
sure to confirm the Anesthesia Delivery settings.
Procedure for Use
1) From the Anest Run Screen, touch Setup > Anesthesia to enter syringe settings:
Setting Action
Remove Syringe Moves block up
Prime Syringe Moves block down
Syringe Size Enter the size of the syringe
Type Anest Enter the type of anesthetic
agent
Calibrate Syringe Resets the factory syringe
calibration (preset)

9
2) Touch Setup > Air Source to choose the carrier gas source.
Room Air: Utilizes the SomnoSuite’s internal pump to draw in surrounding air
Compressed gas: Utilizes a 10-15 PSI gas source connected to the Compress
Gas Port.
3) Install the filled syringe into the syringe block holder, tightening the Anesthetic
Delivery tube to the syringe tip.
4) Be sure that the L—bracket is tightened over the metal collar of the syringe
rather than the glass barrel.
Tip:
Use Remove to move the block up if necessary.
1) Touch Back, then Return to view the Anest Run Screen.
2) Touch and hold Prime to move the block towards the syringe plunger.
Stop when the block touches the top of the plunger.
CAUTION
Do not overprime the delivery tube! Do not allow the pusher
block to depress the syringe plunger or inject liquid anesthetic
into the vaporizer block during this step.
Turn Dial B while holding the Prime button to adjust priming
speed as needed.

10
3) Touch Deliver to enable anesthetic delivery.
4) Place the animal in the induction chamber.
5) From the Anest Run Screen, touch Induction.
Tip:
The SomnoSuite will deliver at a preset flow rate of 500mL/min.
Try manually increasing the flow rate to reduce induction time.
Press then turn Dial A to adjust the gas flow.
6) Turn the Anesthesia Concentration Knob to the desired concentration for
induction. The concentration value appears on the Anest Run Screen as you
adjust the knob.
7) When the animal is suciently anesthetized, touch Induction to stop the air flow.
8) If you want to flush the chamber, touch Flush.
The Flush function automatically flushes the chamber with
0% anesthetic for 10 seconds and stops automatically.
You can repeat the Flush for larger induction chambers or you
can touch Setup > Flush Time to increase the flush.
9) When the flush ends, adjust the tubing clamps to direct airflow to the animal.
10) Open the chamber and remove the animal. Immediately place the animal on
the nose cone or an external ventilator. If you are using 2- or 3-Accessory
Connectors, use the colored clamps to direct the airflow.
11) Touch Nose Cone to resume anesthesia delivery.
12) Turn the Anesthesia Concentration Knob to the desired concentration.

11
13) At the end of the procedure, reduce the anesthetic concentration to 0%.
14)Touch Nose Cone again to stop air flow. Allow the animal to recover.
Tip: Some mask styles have more deadspace than others. If
you notice that the anesthetic depth is too light, try manually
increasing the flow rate.
Press then turn Dial A to adjust the gas flow. Refer to page 47
for recommended flow rates.
If anesthetizing more than one animal, use the highest
recommended flow rate multiplied by two.
Syringe Empty Position Calibration
The SomnoSuite syringe empty points are pre-calibrated. If you need to change the fac-
tory syringe calibrations:
1. Touch
1) the Anest Setup button
2) Anesthesia
3) Calibrate Syringe
Place an empty, fully depressed syringe into the holding block.
2. Press Dial B to begin moving the pusher block. Turn Dial B to adjust the pusher
block speed. When the block touches the top of the syringe plunger, press B
again to stop the block.
3. Press B again to save the new empty point.

12
SomnoSuite® Troubleshooting
• Increase the flow rate in Anest Setup >
Nose Cone Min Vol or Induction Min Vol
• Verify that the syringe size setting is
correctly entered.
• Check that the Anesthetic Delivery Tube
is tightly connected to the Syringe.
• Try using a dierent syringe to see if
the problem improves.
Problem Possible Cause Solutions
Anesthetic depth too
light, or the animal
wakes up
Anesthetic depth too
deep
Minute volume displays
0mL/min.
Pusher block isn’t
moving and Anest%
shows “--%”.
Anesthetic agent
remains in syringe
although SomnoSuite
shows 0mL VolLeft.
• Flow rate is too low
• Incorrect syringe size
• Anesthetic Delivery Tube
or Syringe is leaking
• SomnoSuite was
incorrectly primed
• Flow rate is too low
• Incorrect syringe size
• Incorrect Air Source setting
• Blockage in tubing connection
• Compressed gas source not
turned on or turned on at
incorrect pressure
• Airflow not turned on
• Anesthesia is disabled
• Syringe Empty point
does not match syringe
dimensions
• Using incorrect syringe
• Take care not to overprime the SomnoSuite.
Liquid isoflurane should never be injected
into the vaporizer block during priming.
• Increase the flow rate by pressing and then
turning Dial A.
• Verify that the syringe size setting is
correctly entered.
• Check that Air Source is set correctly, either
to “Room air” or “Compressed Gas”.
• Check all connections and ports for
blockages.
• If using compressed gas, be sure the gas
source is turned on and flowing properly.
• Pressure should be between 5 and 10PSI.
• Check that airflow is turned on, indicated
by the word “Running” on the bottom right
corner of the screen.
• Be sure that the Deliver setting is selected
from the Anest Run Screen.
• Ensure that you are using the glass syringe
included with your SomnoSuite. No other
syringe should be used.
• Ensure that the Syringe Size setting is
correct.
• Re-calibrate the Syringe Empty Position

13
System Customization
Custom Run Screen
To congure a Custom Run Screen:
1) From any Run Screen, touch Menu, then Config.
i Param Locations
Parameter locations allow you to decide where each parameter will be
displayed on the screen. Turn Dial A to scroll through the list of available
parameters, and turn Dial B to assign it to a location.
Press Dial A when finished.
ii Charts
Charts allow you to customize the graph that is displayed on the lower
portion of the Custom Run Screen. You can customize two independent
charts. Choose Chart Type to assign the parameter to be graphed, and
Period to assign a graph time point.
Touch Back when finished.
iii Set Ranges
The Range setting allows you to customize the graph axis. The Ranges set
here also apply to Analog Out. Turn Dial A to scroll through the list of
available parameters, and then turn Dial B to adjust the range.
Press Dial A when finished.
SomnoSuite allows you to configure your own Run Screen, allowing a combination of
parameters from any installed modules to appear on a single screen.
When using a Custom Run Screen, all settings need to be
entered from the Menu or configured with Dial A and B.
See Dial Functions on page 14.

14
iv Dial Menu
The Dial Menu allows you to assign a dial command for your Custom Run
Screen. Turn Dial A to choose a setting you want to control for Dial A.
Touch >> to assign a setting to Dial B.
Touch Back when finished.
Small text on the run screen
displays the assigned Parameter
name for each Dial, if set, and its
current value.
Run Screen with Dial Function Text
To use a dial command:
1. At the Run Screen, quickly turn the Dial assigned to the setting whose value
you want to change. SomnoSuite beeps and the associated text becomes
blue.
2. Turn the Dial to increase or decrease the value for the assigned setting.
After 3 seconds, SomnoSuite beeps again and the command text returns
to black.
As you turn a Dial, setting changes takes place immediately.

15
Alarms
SomnoSuite alarms alert you to certain conditions that might require attention while
SomnoSuite is running.
There are two levels of alarms:
Level Based On Banner Sound (If On) Configurable
Warning Parameters
or other
operating
conditions
Yellow 1-beep pattern Yes
Serious Parameters
or other
operating
conditions
Red 3-beep pattern Yes except for
system alarms
To congure a new Parameter alarm:
1) From any Run Screen, touch Menu then Config.
2) Touch Data Mgmt, then Alarms.
i Silence all Alarms
If you do not wish for the SomnoSuite to make an audible tone when
an alarm is triggered, you can silence them. Turn Dial B to choose
“Silence all Alarms” or “Allow Sound”. Touch Back when finished.
ii Parameter 1-4
Set a custom alarm based on any measured parameter. You may
choose up to 4. Press A when finished configuring the following
settings:
• Parameters: Choose a parameter to monitor by alarm
• Action: Choose how the alarm will alert you
(message and/or sound)
• Type: Choose status to trigger alarm
• Value: Set the threshold value
• Delay: Choose how long the value must meet the threshold
before triggering the alarm
• Clear: Choose how the alarm needs to be cleared

16
iii Fixed Alarms
The SomnoSuite includes a number of fixed alarms that alert you to
scenarios that require attention. The number of Fixed Alarms are
dependent on the number of modules installed. You may view and
configure any of the Fixed Alarms by selecting it, then pressing
Dial A to enter the Alarm Menu.
To clear a sounding alarm:
A triggered alarm produces a colored banner at the bottom of the screen describing the
cause of the alarm.
To silence the alarm:
1) Navigate to the corresponding Screen
2) Touch Setup, then Silence Alarm
OR
1) Touch Menu from any Run Screen
2) Touch Silence Alarm