Labconco CentriVap 78110-00 User manual

CentriVap® Centrifugal
Concentrators and Cold Traps
Models
78100-00 78110-00 79820-00
78100-01 78110-01 79820-01
78100-02 74600-00 79830-00
78100-03 74600-01 79830-01
79840-00
79840-01
74750-00
74750-01
User’s Manual
LABCONCO CORPORATION
8811 PROSPECT AVENUE
KANSAS CITY, MO 64132-2696
800-821-5525, 816-333-8811
FAX [phone]
E-MAIL [email protected]
HOME PAGE www.labconco.com
Labconco’s Mascot,
Labby the LABster
Register your product online at
www.labconco.com/productreg.html
or return the attached card and
receive FREE LabbyWear™!

Copyright Information
Copyright © 2000 Labconco Corporation. All rights reserved.
Labconco Corporation
8811 Prospect Avenue
Kansas City, Missouri 64132
The information contained in this manual and the accompanying product are copyrighted and all rights are
reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design
changes without obligation to notify any person or entity of such change.
CentriVap® is a registered trademark of Labconco Corporation.
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment.
The warranty for all Labconco products will expire one year from date of installation or two years from
date of shipment from Labconco, whichever is sooner, except the following:
• Purifier® Delta™ Series Biological Safety Cabinets, which carry a three-year warranty from date
of installation or four years from date of shipment from Labconco, whichever is sooner.
• Carts carry a lifetime warranty.
• Glassware is not warranted from breakage when dropped or mishandled.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the event of a
warranty claim, contact Labconco Corporation or the dealer who sold you the product. If the cause is
determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or replace all
defective parts to restore the unit to operation. Under no circumstances shall Labconco Corporation be
liable for indirect, consequential, or special damages of any kind. This statement may be altered by a
specific published amendment. No individual has authorization to alter the provisions of this warranty
policy or its amendments. Lamps and filters are not covered by this warranty. Damage due to corrosion or
accidental breakage is also not covered.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical
assistance, contact Labconco’s Customer Service Department or Labconco’s Product
Service Department at 1-800-821-5525 or 1-816-333-8811, between the hours of 7:00
a.m. and 6:00 p.m., Central Standard Time.
Visit Labconco’s web site at: http://www.labconco.com or email Labconco at: [email protected].
Part #7461400, Rev. C
ECO A936
☞

T
TA
AB BL LE E O
OF
F C
CO
ON NT TE EN NT TS S
CHAPTER 1: INTRODUCTION 1
About This Manual 2
Typographical Conventions 3
Your Next Step 4
CHAPTER 2: PREREQUISITES 5
Electrical Requirements 6
Location and Exhaust Requirements 6
Vacuum Pump Requirements 7
Vacuum Line Traps 8
Space Requirements 8
Your Next Step 8
CHAPTER 3: GETTING STARTED 9
Unpacking Your CentriVap 10
CentriVap Components 10
Setting Up Your CentriVap 13
Emergency Access Into the Chamber 13
Component Orientation & Hose Connections 13
Exhaust Port 15
Electrical Connection 15
Rotor Installation 15
Ground Wire 15
Solvent Safety Precautions 16
Your Next Step 18
CHAPTER 4: USING YOUR CENTRIVAP 19
Planning 20
Glassware Selection 20
Loading Glassware Into the CentriVap 20
Heat Setting Guidelines 20
Time Setting Guidelines 21
Heat Boost Operation 21

CentriVap Controls 22
Operating the CentriVap Controls 23
Operating The CentriVap 24
Operational Notes 25
Interrupting a Cycle After it Has Begun 26
CHAPTER 5: MAINTAINING YOUR CENTRIVAP 27
CHAPTER 6: MODIFYING YOUR CENTRIVAP 29
Installing a Glass Lid 29
Installing a Secondary Chemical Trap 30
Installing a CentriZap Strobe Light 32
Installing a Glass Trap in the Cold Trap 33
CHAPTER 7: TROUBLESHOOTING 35
APPENDIX A: CENTRIVAP COMPONENTS 39
APPENDIX B: CENTRIVAP DIMENSIONS 47
APPENDIX C: CENTRIVAP SPECIFICATIONS 51
Wiring Diagram Concentrator (115V) 53
Wiring Diagram Concentrator (230V) 54
Wiring Diagram Cold Trap (–55°) (115V & 230V) 55
Evaporation Rates 56
APPENDIX D: CENTRIVAP ACCESSORIES 59
DECLARATION OF CONFORMITY 63

1
Product Service Domestic 1-800-522-7658, International 816-333-8811
C
CH
HA AP PT TE ER R 1
1
I
IN
NT TR RO OD DU UC CT TI IO ON N
Congratulations on your purchase of a Labconco
CentriVap Concentrator System. Models are available
for operation on 115V or 230V.
The CentriVap Concentrator, when combined with the
CentriVap Cold Trap, uses centrifugal force with heat
and vacuum to rapidly evaporate and condense solvents
from biological and analytical samples. Centrifugation
eliminates bumping and foaming as vacuum is applied
and also concentrates the solute in the bottom of the
vial. This allows recovery of solutes from sample
volumes as small as a few microliters. The
Concentrator is equipped with a 300 watt heater to
speed evaporation by warming the chamber during
processing. A timed outlet for the vacuum pump
connection delays the start of the pump until the rotor
reaches operating speed. A safety switch prevents the
concentrator from starting when the lid is in the open
position and a latch prevents the lid from being opened
while the rotor is turning. The compact, low profile
design allows the Concentrator to be placed on a bench
top or other laboratory work surface.
The microprocessor which controls the operation of the
heater and the motor provides excellent regulation and
reproducibility of protocols. The end of the run can be
signaled by a manually preset timer. Nine different
protocols can be stored in memory so exact test
parameters can be easily repeated.

2 Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 1: Introduction
As the companion to the Concentrator, the CentriVap
Cold Trap protects the vacuum pump by trapping
moisture, vapors and corrosive fumes as they evaporate
from the samples. The CFC free refrigeration system
reduce the temperature of the Cold Trap to –55°C. The
stainless steel trap is used for aqueous and organic
applications. For corrosive applications, the optional
Glass Trap insert should be used. The 230V models
comply with CE regulations.
About This Manual
This manual is designed to help you learn how to
install, use, and maintain your CentriVap. Instructions
for performing routine maintenance and making minor
modifications to your CentriVap are also included.
Chapter 1: Introduction provides a brief overview of
the CentriVap, explains the organization of the manual,
and defines the typographical conventions used in the
manual.
Chapter 2: Prerequisites explains what you need to do
to prepare your site before you install your CentriVap.
Electrical and exhaust requirements are discussed.
Chapter 3: Getting Started contains the information
you need to properly unpack, inspect and install your
CentriVap.
Chapter 4: Using Your CentriVap discusses the basic
operation of your CentriVap. Information on how to
arrange the samples inside your CentriVap and select an
operating cycle is included.
Chapter 5: Maintaining Your CentriVap explains how
to perform routine maintenance on your CentriVap.
Chapter 6: Modifying Your CentriVap describes how to
install the optional glass lid.

3
Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 1: Introduction
Chapter 7: Troubleshooting contains a table of
problems you may encounter while using your
CentriVap, including the probable causes of the
problems, and suggested corrective actions.
Appendix A: CentriVap Components contains labeled
diagrams of the components of the CentriVap.
Appendix B: CentriVap Dimensions contains
comprehensive diagrams showing the dimensions for
the CentriVap.
Appendix C: CentriVap Specifications contains product
specifications. Wiring diagrams for both the 115V and
230V CentriVap are also included.
Appendix D: CentriVap Accessories lists the part
numbers and descriptions of all of the accessories
available for your CentriVap.
Typographical Conventions
Recognizing the following typographical conventions
will help you understand and use this manual:
• Book, chapter, and section titles are shown in italic
type (e.g., Chapter 3: Getting Started).
• Steps required to perform a task are presented in a
numbered format.
• Comments located in the margins provide
suggestions, reminders, and references.
• Critical information is presented in boldface type in
paragraphs that are preceded by the exclamation
icon. Failure to comply with the information
following an exclamation icon may result in injury
to the user or permanent damage to your CentriVap.
• Important information is presented in capitalized
type in paragraphs that are preceded by the pointer
icon. It is imperative that the information contained
in these paragraphs be thoroughly read and
understood by the user.
!
☞

4 Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 1: Introduction
Your Next Step
If your CentriVap needs to be installed, proceed to
Chapter 2: Prerequisites to ensure your installation site
meets all of the requirements. Then, go to Chapter 3:
Getting Started for instructions on how to install your
CentriVap and make all of the necessary connections.
For information on the operational characteristics of
your CentriVap, go to Chapter 4: Using Your
CentriVap.
If your CentriVap is installed and you need to perform
routine maintenance on the CentriVap, proceed to
Chapter 5: Maintaining Your CentriVap.
For information on making modifications to the
configuration of your CentriVap go to Chapter 6:
Modifying Your CentriVap.
Refer to Chapter 7: Troubleshooting if you are
experiencing problems with your CentriVap.

5
Product Service Domestic 1-800-522-7658, International 816-333-8811
C
CH
HA AP PT TE ER R 2
2
P
PR
RE ER RE EQ QU UI IS SI IT TE ES S
Before you install your CentriVap, you need to prepare
your site for installation. Carefully examine the
location where you intend to install your CentriVap.
You must be certain that the area is level and of solid
construction. In addition, an exhaust means must be
provided. An electrical source must be located near the
installation site.
Carefully read this chapter to learn:
• the electrical supply requirements.
• the exhaust requirements.
• the vacuum pump requirements.
Refer to Appendix C: CentriVap Specifications for
complete CentriVap electrical and environmental
conditions, specifications and requirements.
The CentriVap has not been evaluated
by an approval agency for the use of
biological, radiotoxins or flammable
liquids or materials.
!

6 Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 2: Prerequisites
Electrical Requirements
The CentriVap Concentrator requires a dedicated
grounded electrical outlet. This outlet requires a 15
Amp circuit breaker or fuse for models rated at 115V
(60 Hz). An 8 Amp circuit breaker or fuse is required
for models rated at 230V (50/60 Hz). 115V models are
equipped with a 15 Amp NEMA 5-15P plug. 230V
models are not equipped with a plug. It will be
necessary to install a plug to match the available
receptacle.
The CentriVap Cold Trap requires a dedicated electrical
outlet. Models 74600-00 and 78110-00 require a 15
Amp circuit breaker or fuse rated at 115V (60 Hz).
Models 74600-01 and 78110-01 require an 8 Amp
circuit breaker or fuse rated at 230V (50/60 Hz). 115V
models are equipped with a 15 Amp NEMA 5-15P
plug. 230V models are not equipped with a plug. It
will be necessary to install a plug to match the available
receptacle.
It is recommended that an emergency switch for
disconnecting the mains in the case of a malfunction be
located remote from the CentriVap, preferably outside
the room in which the CentriVap is housed, or adjacent
to the exit from that room.
Location and Exhaust
Requirements
The CentriVap System should be located within a fume
hood if hazardous or flammable solvents are used. In
all cases, regardless of the solvent used, it is strongly
recommended that the vacuum pump is vented in a
fume hood. An accessory secondary trap is available to
minimize the exhausting of solvents into the
atmosphere. This does not, however, negate the need to
exhaust the vacuum pump into a fume hood.

7
Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 2: Prerequisites
Vacuum Pump
Requirements
A vacuum pump must be provided by the user. A
vacuum pump with a free air flow rate of 113 liters per
minute and 0.2 x 10-3 mbar blank off pressure is
adequate for aqueous samples. More volatile samples
can be satisfactorily processed using a diaphragm pump
with a free airflow of 42 liters per minute and 200 mbar
vacuum. The inlet fitting on the vacuum pump must be
suitable for 0.50 I.D. hose.
Vacuum pumps used with 115V models should be
equipped with a 115V, 15 Amp NEMA 5-15P plug.
Vacuum pumps used with 230V models should be
equipped with a “reverse” IEC 320 plug. This will
allow the vacuum pump to be plugged into the
receptacle on the back of the CentriVap.
TO INSURE THAT AGGRESSIVE
SAMPLES USED IN THE
CENTRIVAP DO NOT DAMAGE THE
VACUUM PUMP, IT IS
RECOMMENDED THAT ALL
INTERNAL WETTED PARTS ARE
TEFLON®* OR TEFLON COATED.
CAUTION: When selecting the
vacuum pump it is very important to
consider the flammability of the
solvent that will be used. If the
solvents are flammable, an explosion
proof vacuum pump or one suitable
for the solvents to be processed is
recommended.
!
☞
*Teflon® is a registered trademark of DuPont

8 Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 2: Prerequisites
Vacuum Line Traps
When using a mechanical pump which is not corrosion
resistant, the CentriVap Cold Trap or equivalent trap
must be used in the vacuum line to prevent damage
from solvent vapors. When evaporating acids, it is
advisable to include a soda lime acid secondary trap in
the vacuum line. This adds additional protection for the
pump.
Several components within the
CentriVap are made from stainless
steel or aluminum and can be
degraded if exposed to acids. Before
evaporating acids, be sure to contact
Labconco.
A moisture trap, solvent trap and radioisotope trap are
also available. Refer to Appendix D: CentriVap
Accessories for ordering information.
Space Requirements
Refer to Appendix C: CentriVap Specifications for
dimensional drawings of the CentriVap.
Your Next Step
After you have determined that the location for your
CentriVap accommodates the installation and
operational requirements, you are ready to unpack and
install your CentriVap. Proceed to Chapter 3: Getting
Started.
!

9
Product Service Domestic 1-800-522-7658, International 816-333-8811
C
CH
HA AP PT TE ER R 3
3
G
GE
ET TT TI IN NG G S
ST
TA AR RT TE ED D
Now that the site for your CentriVap is properly
prepared, you are ready to unpack, inspect, install, and
test your CentriVap. Read this chapter to learn how to:
• unpack and move your CentriVap.
• set up your CentriVap.
• connect the electrical supply source to your
CentriVap.
• properly exhaust your CentriVap.
• safely use solvents with your CentriVap.
The CentriVap Concentrator weighs
over 42 lbs. (19 Kg). The carton
allows for lifting with a mechanical lift
truck or hand truck. If you must lift
the CentriVap manually, use at least
two (2) persons and follow safe lifting
guidelines.
The CentriVap Cold Trap weighs over
70 lbs. (33 Kg). The carton allows for
lifting with a mechanical lift truck or
hand truck. If you must lift the Cold
Trap manually, use at least two (2)
persons and follow safe lifting
guidelines.
!
!!
!

10 Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 3: Getting Started
Unpacking Your CentriVap
Carefully unpack your CentriVap and inspect it for
damage that may have occurred in transit. If your
CentriVap is damaged, notify the delivery carrier
immediately and retain the entire shipment intact for
inspection by the carrier.
DO NOT RETURN GOODS
WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO.
UNAUTHORIZED RETURNS WILL
NOT BE ACCEPTED.
IF YOUR CENTRIVAP WAS
DAMAGED IN TRANSIT, YOU
MUST FILE A CLAIM DIRECTLY
WITH THE FREIGHT CARRIER.
LABCONCO CORPORATION AND
ITS DEALERS ARE NOT
RESPONSIBLE FOR SHIPPING
DAMAGE.
Do not discard the carton or packing material for your
CentriVap until you have checked all of the
components and installed and tested the CentriVap.
CentriVap Components
As previously mentioned, the CentriVap System is
available in 115V or 230V. Locate the model of
CentriVap you received in the following table. Verify
that the components listed are present and undamaged.
The United States
Interstate Commerce
Commission rules
require that claims be
filed with the delivery
carrier within fifteen (15)
days of delivery.
☞
☞

11
Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 3: Getting Started
Catalog # Product Description
78100-00 CentriVap Concentrator – 115V
78100-10 CentriVap Concentrator – 230V
78100-02 CentriVap Concentrator with Heat Boost – 115V
78100-03 CentriVap Concentrator with Heat Boost – 230V
Plus the Following:
Part # Component Description
74614-00 User’s Manual
13345-00
13341-00
Power Cord – 115V
or
Power Cord – 230V
78286-06 Tubing
14888-00 Clamp (2)
Catalog # Product Description
78110-00 CentriVap Cold Trap – 115V
78110-01 CentriVap Cold Trap – 230V
Plus the Following:
Part # Component Description
74614-00 User’s Manual
13345-00
13341-00
Power Cord – 115V
or
Power Cord – 230V
74646-00 Wire Assembly
78286-06 Tubing
14888-00 Clamp (2)
79814-00 Insulation – Cover
78735-00 Cover Assembly
Catalog # Product Description
74600-00 CentriVap Cold Trap – 115V
74600-01 CentriVap Cold Trap – 230V
Plus the Following:
Part # Component Description
74614-00 User’s Manual
13345-00
13341-00
Power Cord – 115V
or
Power Cord – 230V
74646-00 Wire Assembly
78286-06 Tubing
14888-00 Clamp (2)
79814-00 Insulation – Cover
78735-00 Cover Assembly
Concentrator
Cold Trap

12 Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 3: Getting Started
Catalog # Product Description
79820-00 Aqueous System – 115V
79820-01 Aqueous System – 230V
Plus the Following:
Part # Component Description
78100-00
78100-01
CentriVap Concentrator – 115V
or
CentriVap Concentrator – 230V
78110-00
78110-01
CentriVap Cold Trap – 115V
or
CentriVap Cold Trap – 230V
74609-00 Cannister Assembly
78149-00 Cartridge – Moisture Trap
74550-00 Rotor – 15ml
Catalog # Product Description
79830-00 Acid System – 115V
79830-01 Acid System – 230V
Plus the Following:
Part # Component Description
78100-00
78100-01
CentriVap Concentrator – 115V
or
CentriVap Concentrator – 230V
78110-00
78110-01
CentriVap Cold Trap – 115V
or
CentriVap Cold Trap – 230V
74609-00 Cannister Assembly
78148-00 Cartridge – Acid Trap
74550-00 Rotor – 15ml
78734-00 Glass Trap Assembly
Catalog # Product Description
79840-00 Solvent System – 115V
79840-01 Solvent System – 230V
Plus the Following:
Part # Component Description
78100-00
78100-01
CentriVap Concentrator – 115V
or
CentriVap Concentrator – 230V
78110-00
78110-01
CentriVap Cold Trap – 115V
or
CentriVap Cold Trap – 230V
74609-00 Cannister Assembly
78152-00 Cartridge – Solvent Trap
74550-00 Rotor – 15ml
Systems

13
Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 3: Getting Started
If you do not receive one or more of the components
listed for your CentriVap, or if any of the components
are damaged, contact Labconco Corporation
immediately for further instructions.
Setting Up Your CentriVap
After you verify receipt of the proper components,
move your CentriVap to the level and sturdy location
where you want to install it. There is no need to secure
the CentriVap to the work surface. Then, follow the
steps listed below.
Emergency Access Into the
Chamber
The CentriVap is designed to prevent access to the
chamber in the event of a power disruption. If it is
necessary to open the lid when there is no electrical
power connected to the CentriVap, insert a small
screwdriver or similar instrument into the small round
hole on the left side of the case near the top behind the
control panel. This will unlock the lid latch
mechanism. While holding the screwdriver in place,
raise the lid with the other hand.
Never attempt to defeat the latch or
open the lid while the CentriVap is
running.
Component Orientation & Hose
Connections
The relative position of the CentriVap Concentrator,
Cold Trap and the vacuum pump may be as shown or
may be reversed. The refrigeration system in the Cold
Trap exhausts air through the rear of the cabinet. A
minimum of 3" should be allowed between the back of
the Cold Trap and the adjacent wall surface and
between the sides of the Cold Trap and the adjacent
!

14 Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 3: Getting Started
wall surfaces. Restriction of the airflow through the
cabinet during operation could adversely affect
performance.
After positioning the components, it is necessary to join
the system together using the hoses provided. Attach
one hose to the .50 diameter tube that extends out the
back of the Concentrator. Attach the other end of this
hose to one of the barb fittings on the Cold Trap Cover
Assembly. Clamp securely with the clamps supplied.
Attach another hose to the remaining barb fitting on the
Cold Trap Cover Assembly and clamp securely. If the
accessory Secondary Trap is not used, attach the other
end of the hose to the inlet port on the vacuum pump.
If the accessory Secondary Trap is used, install the
Secondary Trap as explained in Chapter 6: Modifying
Your CentriVap. Then attach the hose from the Cold
Trap Cover Assembly to the “out” connector on the
Secondary Trap
Concentrator
Cold Trap
Vacuum Pump

15
Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 3: Getting Started
Secondary Trap. Clamp securely. Attach another hose
from the remaining connector on the Secondary Trap to
the inlet port on the vacuum pump and clamp securely.
Exhaust Port
If the CentriVap is not located in a fume hood, attach
one end of a user supplied hose to the exhaust port on
the vacuum pump. Clamp securely. Route the other
end to a fume hood or other laboratory ventilation
device.
Electrical Connection
Plug the power cord into the receptacle on the back of
the CentriVap Concentrator and plug the other end into
a suitable power receptacle. Plug the power cord into
the receptacle on the back of the CentriVap Cold Trap
and plug the other end into a suitable power receptacle.
Plug the power cord from the vacuum pump into the
receptacle on the back of the Concentrator. If the
vacuum pump has an off/on switch, turn the switch on.
The vacuum pump will be controlled by the
Concentrator.
Rotor Installation
Place the rotor onto the shaft of the Concentrator.
Rotate the rotor slightly to engage the drive pin in the
shaft with the slots in the rotor hub. The top of the
shaft should be in line with the top of the rotor hub.
Do not use a rotor if it shows any signs
of damage.
!

16 Product Service Domestic 1-800-522-7658, International 816-333-8811
Chapter 3: Getting Started
Ground Wire
Attach one end of the included ground wire to the
stainless steel elbow on the drain tubing on the left side
of the Cold Trap.
When draining the CentriVap Cold
Trap always attach the other end of
the grounding clip to the solvent catch
pot to eliminate the risk of
electrostatic spark ignition.
Solvent Safety
Precautions
The CentriVap is not classified as
“explosion proof.” It has been
designed with safety as a primary
consideration and should be used in a
prudent manner using “good
laboratory practices.” It has been
designed for use with compounds as
described in the United States
National Electrical Code Class I,
Group D. The heater may be
programmed to run as hot as 100°C,
however, the heater element may
normally run at 110°C. A thermal
fuse limits the heater to a maximum
temperature of 141°C. It is important
that the solvents used are compatible
with these temperatures. Do not
evaporate solvents that have an
autoignition temperature below
180°C. Do not evaporate solvents that
are classified as Group A, B, or C by
the National Electrical Code.
Evaporate only non-flammable or
Group D solvents with autoignition
temperatures 180°C or above. Use of
other compounds could cause an
explosion.
!
!!
!
!