Labconco CentriVap DNA Systems User manual

CentriVapDNA Systems
Models
7970010
7970011
7970030
7970035
7970037

Copyright © 2018 Labconco Corporation. The information contained in this manual and the accompanying
products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves
the right to make periodic design changes without obligation to notify any person or entity of such change.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier.
Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525
or 1-816-333-8811, between the hours of 7:30 a.m. and 5:30 p.m., Central Standard Time.
Part #7397609, Rev. D
ECO L447
Warranty
Labconco Corporation provides a warranty to the original buyer for the repair or replacement of parts and
reasonable labor as a result of normal and proper use of the equipment with compatible chemicals. Broken
glassware and maintenance items, such as filters, gaskets, light bulbs, finishes and lubrication are not
warranted. Excluded from warranty are products with improper installation, erratic electrical or utility supply,
unauthorized repair and products used with incompatible chemicals.
The warranty for CentriVap®DNA Systems will expire one year from date of installation or two years from
date of shipment from Labconco, whichever is sooner. Warranty is non-transferable and only applies to the
owner (organization) of record.
Buyer is exclusively responsible for the set-up, installation, verification, decontamination or calibration of
equipment. This limited warranty covers parts and labor, but not transportation and insurance charges. If the
failure is determined to be covered under this warranty, the dealer or Labconco Corporation will authorize
repair or replacement of all defective parts to restore the unit to operation. Repairs may be completed by 3rd
party service agents approved by Labconco Corporation. Labconco Corporation reserves the rights to limit this
warranty based on a service agent’s travel, working hours, the site’s entry restrictions and unobstructed access
to serviceable components of the product.
Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages
of any kind. This warranty is exclusive and in lieu of all other warranties whether oral, or implied.

TABLE OF CONTENTS
CHAPTER 1: INTRODUCTION 1
Safety Symbols 2
CHAPTER 2: PREREQUISITES 3
Electrical Requirements 3
Location and Exhaust Requirements 4
Space Requirements 4
CHAPTER 3: GETTING STARTED 5
Unpacking Your CentriVap 6
CentriVap Components 6
Setting Up Your CentriVap 7
Emergency Access Into the Chamber 7
Exhaust Port 7
Electrical Connection 7
Rotor Installation 7
Chemical Resistance of CentriVap Components 8
Solvent Safety Precautions 9
CHAPTER 4: USING YOUR CENTRIVAP 11
Planning 12
Glassware Selection 12
Loading Glassware Into the CentriVap 12
Temperature Setting Guidelines 12
Time Setting Guidelines 12
CentriVap Controls 13
Operating the CentriVap Controls 14
Operating the CentriVap 15
Operational Notes 16
Interrupting a Cycle After it Has Begun 16
Gas Ballast 16
Safety Precautions 17
CHAPTER 5: MAINTAINING YOUR CENTRIVAP 18

CHAPTER 6: ACCESSORIES FOR YOUR CENTRIVAP 20
Installing a Glass Lid 22
Installing a Secondary Chemical Trap 22
Installing a Vacuum Gauge 23
Installing a CentriZap Strobe Light 24
CHAPTER 7: TROUBLESHOOTING 25
APPENDIX A: CENTRIVAP COMPONENTS 27
APPENDIX B: CENTRIVAP DIMENSIONS 29
APPENDIX C: CENTRIVAP SPECIFICATIONS 30
Electrical Specifications 30
Environmental Conditions 30
Evaporation Rates 31

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Chapter 1:
Introduction
Congratulations on your purchase of a Labconco CentriVap DNA Centrifugal
Concentrator . Models are available for operation on 115V or 230V.
The CentriVap Concentrator uses centrifugal force with heat and vacuum to
rapidly dry precipitates of DNA and RNA samples. Centrifugation eliminates
bumping and foaming as vacuum is applied and also concentrates the solute in the
bottom of the vial. This allows recovery of solutes from sample volumes as small
as a few microliters. The concentrator is equipped with a 300 watt heater to speed
evaporation by warming the chamber during processing. A timed outlet for the
vacuum pump connection delays the start of the pump until the rotor reaches
operating speed. A safety switch prevents the concentrator from starting when the
lid is in the open position and a latch prevents the lid from being opened while the
rotor is turning. The compact, low profile design allows the concentrator to be
placed on a bench top or other laboratory work surface.
All wetted parts within the pump are PTFE, or other chemical resistant
fluoropolymers. A glass trap is provided to remove liquid from the system before
it enters the pump and a second glass trap is provided on the outlet side of the
pump to trap liquid expelled from the pump.
The microprocessor, which controls the operation of the heater and the motor,
provides excellent regulation and reproducibility of protocols. A manually preset
timer can signal the end of the run. Nine different protocols can be stored in
memory so exact test parameters can be easily repeated.

Chapter 1: Introduction
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Safety Symbols
Your CentriVap Concentrator was designed with safety in mind, however
conditions may exist that could be hazardous.
Throughout this manual potentially hazardous conditions are identified using the
following words and symbols.
It is important that you understand the warnings listed throughout this manual
before you operate the CentriVap Concentrator
.

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Chapter 2:
Prerequisites
Before you install your CentriVap, you need to prepare your site for installation.
Carefully examine the location where you intend to install your CentriVap. You
must be certain that the area is level and of solid construction. In addition, an
exhaust means must be provided. An electrical source must be located near the
installation site.
Carefully read this chapter to learn:
The electrical supply requirements.
The exhaust requirements.
Refer to Appendix C: CentriVap Specifications for complete CentriVap electrical
and environmental conditions, specifications and requirements.
Electrical Requirements
The CentriVap Concentrator requires a dedicated grounded electrical outlet. This
outlet requires a 15 Amp circuit breaker or fuse for models rated at 115V (60 Hz).
An 8 Amp circuit breaker or fuse is required for models rated at 230V (50/60 Hz).
Various models are available for use in different locations throughout the world.
Each has a unique plug suitable for the specific location. See CentriVap
Components in Chapter 3 for detail plug specifications. If the plug supplied with
the CentriVap Does not match with the available receptacle, remove this plug and
replace it with an approved plug of the suitable style.
It is recommended that an emergency switch for disconnecting the mains in the
case of a malfunction be located remote from the CentriVap, preferably outside
the room in which the CentriVap is housed, or adjacent to the exit from that room.

Chapter 2: Prerequisites
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Location and Exhaust Requirements
The CentriVap Concentrator should be located on a surface that is stable, flat and
level.
WARNING: The CentriVap System should be located within a fume hood if
hazardous or flammable solvents are used. Heating of materials could lead
to the liberation of hazardous gases. In all cases, regardless of the solvent
used, it is strongly recommended that the vacuum pump is vented in a fume
hood. An accessory secondary trap is available to minimize the exhausting of
solvents into the atmosphere. This does not, however, negate the need to
exhaust the vacuum pump into a fume hood. Failure to properly vent the
CentriVap will expose personnel to potentially harmful chemicals.
The CentriVap has not been evaluated by an approval agency for the use of
biological, radio toxins or flammable liquids or materials.
Space Requirements
Refer to Appendix B: CentriVap Dimensions for dimensional drawings of the
CentriVap DNA System.
No person or any hazardous material should be within 12 inches of the CentriVap
Concentrator while it is operating.

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Chapter 3:
Getting Started
Now that the site for your CentriVap is properly prepared, you are ready to
unpack, inspect, install, and test your CentriVap. Read this chapter to learn how
to:
Unpack and move your CentriVap.
Set up your CentriVap.
Connect the electrical supply source to your CentriVap.
Properly exhaust your CentriVap.
Safely use solvents with your CentriVap.
CAUTION: The CentriVap Concentrator weighs over 80 lbs. (36 Kg). The
carton allows for lifting with a mechanical lift truck or hand truck. If you
must lift the CentriVap manually, use at least two (2) persons and follow safe
lifting guidelines.

Chapter 3: Getting Started
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Unpacking Your CentriVap
Carefully unpack your CentriVap and inspect it for damage that may have
occurred in transit. If your CentriVap is damaged, notify the delivery carrier
immediately and retain the entire shipment intact for inspection by the carrier.
The United States Interstate Commerce Commission rules require that claims be
filed with the delivery carrier within fifteen (15) days of delivery.
NOTE: Do not return goods without the prior authorization of Labconco.
Unauthorized returns will not be accepted. If your CentriVap was damaged
in transit, you must file a claim directly with the freight carrier. Labconco
Corporation and its dealers are not responsible for shipping damage.
Do not discard the carton or packing material for your CentriVap until you have
checked all of the components and installed and tested the CentriVap.
CentriVap Components
As previously mentioned, the CentriVap DNA System is available in 115V or
230V. Locate the model of CentriVap you received in the following table. Verify
that the components listed are present and undamaged.
Catalog #
Product Description
7970010
CentriVap DNA Concentrator –115V –USA
1334500
Power Cord with NEMA 5-15P Plug
7462900
Rotor
7397609
User’s Manual
7970011
CentriVap DNA Concentrator –230V –USA
1338000
Power Cord with NEMA 6-15P Plug
7462900
Rotor
7397609
User’s Manual
7970030
CentriVap DNA Concentrator –230V –EU
1336100
Power Cord with CEE 7/7 Plug
7462900
Rotor
7397609
User’s Manual
7970035
CentriVap DNA Concentrator –230V –UK
1332600
Power Cord with BS 1363 Plug
7462900
Rotor
7397609
User’s Manual
7970037
CentriVap DNA Concentrator –230V –China
1332700
Power Cord with CHI-10P Plug
7462900
Rotor
7397609
User’s Manual
If you do not receive one or more of the components listed for your CentriVap, or
if any of the components are damaged, contact Labconco Corporation
immediately for further instructions.

Chapter 3: Getting Started
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Setting Up Your CentriVap
After you verify receipt of the proper components, move your CentriVap to the
level and sturdy location where you want to install it. There is no need to secure
the CentriVap DNA Concentrator to the work surface. Then, follow the steps
listed below.
Emergency Access Into the Chamber
The CentriVap is designed to prevent access to the chamber in the event of a
power disruption. If it is necessary to open the lid when there is no electrical
power connected to the CentriVap, insert a small screwdriver or similar
instrument into the small round hole on the left side of the case near the top
behind the control panel. This will unlock the lid latch mechanism. While
holding the screwdriver in place, raise the lid with the other hand.
CAUTION: Never attempt to defeat the latch or open the lid while the
CentriVap is running. Personnel injury can result from moving parts and
chemicals.
Exhaust Port
It the CentriVap is not located in a fume hood, attach one end of a .50 inch ID
hose to the exhaust port on the left side on the vacuum pump glass trap exhaust
port. Clamp securely. Route the other end to a fume hood or other laboratory
ventilation device.
Electrical Connection
Plug the power cord into the receptacle on the back of the CentriVap Concentrator
and plug the other end into a suitable power receptacle
Rotor Installation
Place the rotor onto the shaft of the Concentrator. Rotate the rotor slightly to
engage the drive pin in the shaft with the slots in the rotor hub. The top of the
shaft should be in line with the top of the rotor hub.
IMPORTANT: Do not use a rotor if it shows any signs of damage.

Chapter 3: Getting Started
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Chemical Resistance of CentriVap
Components
Your CentriVap DNA Centrifugal Concentrator is designed to be chemical
resistant to most compounds that are commonly used in the concentration
processes that are performed in it. However, by necessity, the CentriVap is
comprised of a number of different materials, some of which may be attacked and
degraded by certain chemicals. The degree of degradation is obviously dependent
on the concentration and duration of exposure. Some major components of the
CentriVap that are susceptible to degradation are as follows:
Acids
Acids
Bases Solvents
Bases Solvents
COMPONENT MATERIAL
Acetic Acid 20%
Boric Acid
Formic Acid
Hydrobromic Acid 20%
Hydrochloric Acid 20%
Nitric Acid 20%
Sulfuric Acid 10%
Trifluoroacetic Acid (TFA)
Ammonium Hydroxide
Acetone
Acetonitrile
Chloroform
Dimethyl Formamide
Dimethyl Sulfoxide (DMSO)
Ethanol
Ethyl Acetate
Hexanes
Isoproponal
Methanol
Methylene Chloride
Methyl t-Butyl Ether (MTBE)
Toluene
Water
Standard
CentriVap
Lid Acrylic C D C D D D D C D D C D D
Bearings High Carbon Steel D D D D D D D D D D D
Lid Gasket EPDM D D D D D C C D
Rotor Shaft Stainless Steel D D D
Valve Stainless Steel D D D
O Rings Viton (Fluorocarbon) C C C D C
Fittings Polypropylene D C
Rotor Anodized Aluminum D D
Rotor Hub Acetal (Delrin) C C C D D D C D C
Tubing PVC D C D D D D D D D C D D D D
Acid Resistant
CentriVap
Chamber Teflon coated Aluminum
Lid Glass
Bearings Stainless Steel D D D
Lid Gasket EPDM D D D D D C C D
Rotor Shaft Hastelloy
Valve Teflon D
O Rings Viton (Fluorocarbon) C C C D C
Fittings Polypropylene D C
Rotor Teflon coated Aluminum
Rotor Hub Polypropylene D C
Tubing PVC D C D D D D D D D C D D D D
Cold Trap -50°C
Chamber Stainless Steel D D D
Lid Acrylic C D C D D D D C D D C D D
Cold Trap Gasket Neoprene D D C D D C D D D D D C D
Cold Trap -84°C
& -105°C
Chamber Stainless Steel D D D
Lid Stainless Steel D D D
Cold Trap Gasket Neoprene D D C D D C D D D D D C D
C- Moderate Degradation- Questionable use
D- Severe Degradation- Infrequent use recommended- immediate thorough cleaning required
Epoxy coated Aluminum
Chamber
C
D
C
D

Chapter 3: Getting Started
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Diaphragm vacuum pumps sold by Labconco have wetted parts either made
from PTFE or protected by PTFE coatings and are suitable for nearly all
procedures.
When using compounds in the CentriVap that are hostile to the materials of
construction, it is imperative that the equipment is appropriately maintained.
After each run, clean up all residues, spills and materials that might have
splashed in the chamber using agents suitable for the substance involved.
If the compounds used attack acrylic, consider using the optional glass
chamber lid. Contact Labconco for ordering information.
If optional secondary traps are used, monitor their condition often and
replace them when they are saturated. A new acid trap is off-white and
changes color to purple when used up. A new moisture trap is blue and
changes color to pink when it is used up. The solvent trap molecular sieve
does not change color when saturated so extra care must be taken to
determine when a replacement cartridge should be installed.
Solvent Safety Precautions
CAUTION: The CentriVap is not classified as “explosion proof.” It has
been designed with safety as a primary consideration and should be used in a
prudent manner using “good laboratory practices.” It has been designed
for use with compounds as described in the United States National Electrical
Code Class I, Group D. The heater may be programmed to run as hot as
100°C, however, the heater element may normally run at 110°C. A thermal
fuse limits the heater to a maximum temperature of 141°C. It is important
that the solvents used are compatible with these temperatures. Do not
evaporate solvents that have an autoignition temperature below 180°C. Do
not evaporate solvents that are classified as Group A, B, or C by the
National Electrical Code. Evaporate only non-flammable or Group D
solvents with autoignition temperatures 180°C or above. Use of other
compounds could cause an explosion.

Chapter 3: Getting Started
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CAUTION: Solvents used in the CentriVap may be flammable or hazardous.
Use extreme caution and keep sources of ignition away from the solvents.
When using flammable or hazardous solvents, both the CentriVap and the
vacuum pump should be operated inside a fume hood.
If a sample is spilled in the chamber it must immediately be cleaned up.
Hazardous materials such as strong acids or bases, radioactive substances
and volatile organics, must be handled carefully and promptly cleaned up if
spilled.
Do not store flammable or hazardous solvents within 12 inches (300 mm) of
the CentriVap.
IMPORTANT: The disposal of substances used in connection with this
equipment may be governed by various Federal, State or local regulations.
All users of this equipment are urged to become familiar with any
regulations that apply in the user’s area concerning the dumping of waste
materials in or upon water, land or air and to comply with such regulations.

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Chapter 4:
Using Your CentriVap
After your CentriVap has been installed as detailed in Chapter 3: Getting Started,
you are ready to begin using your CentriVap. Read this chapter to learn how to:
Set operating parameters.
Operate the controls.
Properly select and position glassware inside your CentriVap.
Understand the display.
Interrupt a cycle after it has begun.
NOTE: See Appendix C: CentriVap Specifications, for electrical requirements.
NOTE: Do not store or stack supplies or equipment on top of the CentriVap.

Chapter 4: Using Your CentriVap
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Planning
Thoroughly understand procedures and the equipment operation prior to
beginning work. The unique performance of the CentriVap is dependent upon the
proper balance of heat, vacuum and centrifugal force. If the proper balance is not
established, it is possible to damage or lose a portion of the sample. Therefore, if
you are unfamiliar with the CentriVap or are attempting a new protocol, it may be
helpful to make a trial run that is void of the sample you are attempting to
concentrate.
Glassware Selection
Normally, sample tubes should be filled no more than approximately half full.
Select the size of the sample tube so it is compatible with the rotor and the desired
sample size. Tubes should not be loose in the rotor. Rotors are available with
holes for various size tubes. Refer to Chapter 6: Accessories for your CentriVap
DNA Concentrator for available rotor sizes.
Loading Glassware into the CentriVap
Smooth operation of the CentriVap is dependent upon proper balance of the
machine. Therefore, if less than a full load of samples is run, it is important to
load samples into the CentriVap in a fairly symmetrical manner distributing the
weight of the samples evenly in the sample rotor.
Temperature Setting Guidelines
The evaporation rate achieved by the CentriVap is dependent upon a variety of
factors. These include the nature of the solvent, the temperature and the pressure
in the vacuum system.
As a general guideline, to speed the evaporation process, the CentriVap
temperature should be set as high as possible as long as the temperature will not
damage the sample or cause the sample to bump.
Time Setting Guidelines
The CentriVap Concentrator has two timers. The “RUN TIME” turns the entire
concentrator off after the user set period of time. This stops the rotation, turns off
the heater and vacuum pump and bleeds vacuum from the system. The heater
may be turned off prior to the concentrator turning off so heat-sensitive samples
may be protected from exposure to excessive heat after the solvent has
evaporated. To turn the heater off prior to stopping the entire system, enter a set
time into the memory at the “HEATER TIME.” When the programmed time
expires, the heater will turn off but the rotation and vacuum will continue until the
run time expires.

Chapter 4: Using Your CentriVap
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The tables in Appendix C: CentriVap Specifications indicate approximate times
required to evaporate various common solvents. Actual times must be determined
by the user. The CentriVap can be set to alarm after a preset period of operation.
When the time expires, the CentriVap will give an audible alarm and turn itself
off.
CentriVap Controls
The control panel for the CentriVap is shown below with a description about its
function.
1. Display –The liquid crystal display (LCD) shows set point parameters and
actual measured conditions.
2. Program Buttons –Used to initiate the start of a run with the use of just one
button.
3. Run/Stop Button –Used to start or stop a run.
4. Preheat Button –Used to turn on the heater to preheat the chamber prior to
loading samples.
5. Increase Button –When pressed, the last selected set point will increase.
6. Decrease Button –When pressed, the last selected set point will decrease.
7. Set Point Select Button –To select a parameter to change, press the select
button. Arrows on the display will point to the parameter that may be altered.
4
3
2
1
5
6
7

Chapter 4: Using Your CentriVap
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Operating the CentriVap Controls
Preheat:
To preheat the chamber, press the “PREHEAT” button. The display will show:
SP: XXX ACT: XXX
Press the increase or decrease button until the desired set point (SP) is displayed.
The actual chamber temperature, (ACT) is displayed to the right. When power is
being supplied to the heaters, a bar under the actual temperature will illuminate.
The chamber will continue to preheat until either the “PREHEAT” button is
pressed again or the “RUN” button is pressed.
Select existing program:
Operating parameters can be stored in memory so protocols can be repeated.
Nine programs can be stored. To select a program, press the set point “SELECT”
button until arrows point to the program number indicating that this set point can
be run or altered. To change the program number, press the increase or decrease
button until the desired program number is displayed. When the program number
is changed, all its set points change also to indicate the last entered parameters for
that program.
Store frequently run protocols in program 1, 2, or 3. Then, by pressing “PROG
1,” “PROG 2” or “PROG 3,” the stored program will be initiated without having
to press any other button. Pressing just the one button starts the rotor, the heater,
the timers and the vacuum pump.
Change “Temperature” Set Point:
To change the “Temperature” set point, press the set point “SELECT” button until
arrows point to the “Temperature” set point, which can be changed from - (OFF)
up to 99° by pressing the increase or decrease buttons. The set point is 100°C if
the display shows “HI.” The last entered set point is stored in memory.
Change “Run Time” or “Heater Time” Set Point:
To change the time set point, press the set point “SELECT” button until arrows
point to the “Heater Time” or “Run Time” set point which can be changed from 1
to 999 minutes by pressing the increase or decrease buttons. If it is desired to
have the CentriVap run continuously without alarming at the end of a time period,
press the increase button until the “Run Time” display says “ON.” The last
entered set point is stored in memory.
If during a run the “STOP” button is pressed, the timers remember the time at
which stop occurred. If “RUN” is then pressed, the timers continue to count
down from the time at which they were stopped. If you are running program 1,
program 2 or program 3, pressing “PROG 1,” “PROG 2” or “PROG 3” resets the
timers to the original set point time and the CentriVap starts a new run.

Chapter 4: Using Your CentriVap
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To reset the timers to the original set point time when running programs other
than program 1, program 2 or program 3, press the start button and hold it for five
seconds. The display will indicate that the timer is reset.
Operating the CentriVap
CAUTION: To avoid personnel injury; Do not operate the CentriVap if the
lid is scratched or nicked, or shows signs of damage. A damaged lid could fail
under vacuum.
While the CentriVap Concentrator is operating, do not lean on the lid, do not
stand near it longer than necessary and do not place hazardous materials within 12
inches.
1. Turn “ON” the CentriVap power switch.
2. Select a program or set the set point parameters.
3. Preheat the chamber if desired.
4. Place samples in vials. Normally the vials should be no more than half full.
Place vials in a rotor.
5. Load the rotor with samples into the chamber.
6. Close the lid.
7. Press “RUN.” If the display was showing set point parameters, it will change
to show actual parameters. Press “RUN” again. The “S” (STOP) in the
display changes to “R” (RUN). The program on the display alternately
displays “R” or the number of the program that is running. If you intend to
run program 1, 2 or 3, simply press “PROG 1,” “PROG 2” or “PROG 3” to
quick start the CentriVap. The rotor will start, the vacuum break valve will
close and the vacuum pump will start after the rotor reaches operating speed.
8. Set point parameters can be altered at any time during a run by first selecting
the parameter using the set point “SELECT” button and then pressing the
“INCREASE” or “DECREASE” switch.
9. If the run time set point is used, at the end of the set time an alarm sounds. All
functions cease.

Chapter 4: Using Your CentriVap
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10. Press “STOP” to terminate operation if the CentriVap has not already stopped
itself.
11. When the evaporation is complete, allow the rotor to stop moving, lift the lid
and remove the samples.
Operational Notes
When the CentriVap is turned on, the CentriVap returns to the same mode
(“RUN” or “STOP”) that it was in when the power was turned off. If the
CentriVap was in the “RUN” mode when the power was turned off, when the
power is turned on, the CentriVap attempts to return to the programmed set points
and continue the run.
To reset the timers to the original set point when running other than program 1,
program 2 or program 3, press the start button and hold it for five seconds. The
display will indicate that the timer is reset.
Interrupting a Cycle After it Has Begun
At any time during a run, the cycle may be stopped by pressing the “STOP”
button. This shuts off all operating functions. After the rotor stops, the lid may
be opened. If it is necessary to re-start the CentriVap, close the lid and press
“RUN.” The CentriVap resumes operation at the same set point parameters and
the timer continues to count down from the time at which the CentriVap was
stopped.
Gas Ballast
The vacuum pump incorporated in the CentriVap DNA Concentrator is capable of
achieving an ultimate pressure of <7 MBAR. Some solvents may freeze when
exposed to low pressure. If the sample being processed freezes, evaporation
ceases and the sublimation process occurs, which in turn greatly reduces the
concentration rate. Freezing of the sample may be prevented by opening the gas
ballast valve which increases the ultimate pressure in the system. Pressure may
be monitored using a vacuum gauge as described in Chapter 6: Installing a
Vacuum Gauge.