Labconco FreeZone 70020 Series User manual

FreeZone®Benchtop
Freeze Dryers
User's Manual
Please read the User’s Manual before operating the equipment.
LABCONCO CORPORATION
8811 Prospect Avenue
Kansas City, MO 64132
[phone], [phone]
(816) 363 -0130 fa x
[e-mail]
Models
70020**** 2.5L -50°C Series
71020**** 2.5L -84°C Series
70040**** 4.5L -50°C Series
71040**** 4.5L -84°C Series
72040**** 4.5L -105°C Series
70080**** 8L -50°C Series
T
o receive important product updates,
complete your product registration card
online at register.labconco.com

Original instructions
Copyright © 2017 Labconco Corporation. The information contained in this manual and the accompanying
products are copyrighted and all rights reserved by Labconco Corporation. Labconco Corporation reserves
the right to make periodic design changes without obligation to notify any person or entity of such change.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier.
Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525
or 1-816-333-8811, between the hours of 7:30 a.m. and 5:30 p.m., Central Standard Time.
Part #7343000, Rev. A
ECO K623
Warranty
Labconco Corporation provides a warranty to the original buyer for the repair or replacement of parts and
reasonable labor as a result of normal and proper use of the equipment with compatible chemicals. Broken
glassware and maintenance items, such as filters, gaskets, light bulbs, finishes and lubrication are not
warranted. Excluded from warranty are products with improper installation, erratic electrical or utility supply,
unauthorized repair and products used with incompatible chemicals.
The warranty for FreeZone®Freeze Dry Systems will expire one year from date of installation or two years
from date of shipment from Labconco, whichever is sooner. Warranty is non-transferable and only applies to
the owner (organization) of record.
Buyer is exclusively responsible for the set-up, installation, verification, decontamination or calibration of
equipment. This limited warranty covers parts and labor, but not transportation and insurance charges. If the
failure is determined to be covered under this warranty, the dealer or Labconco Corporation will authorize
repair or replacement of all defective parts to restore the unit to operation. Repairs may be completed by 3rd
party service agents approved by Labconco Corporation. Labconco Corporation reserves the rights to limit this
warranty based on a service agent’s travel, working hours, the site’s entry restrictions and unobstructed access
to serviceable components of the product.
Under no circumstances shall Labconco Corporation be liable for indirect, consequential, or special damages
of any kind. This warranty is exclusive and in lieu of all other warranties whether oral, or implied.

Original instructions
TABLE OF CONTENTS
CHAPTER 1: INTRODUCTION 1
Intended Use 1
Freeze Dry Process 1
Freeze Dry Rates 2
Freeze Dry Capacity 3
Samples Containing Volatile Substances 4
About This Manual 4
Typographical Conventions 5
CHAPTER 2: PREREQUISITES 6
Electrical Requirements 6
Location Requirements 7
Vacuum Pump Requirements 7
Chamber or Manifold Requirements 7
CHAPTER 3: GETTING STARTED 8
Unpacking Your Freeze Dryer 8
Freeze Dryer Components 9
Setting Up Your Freeze Dryer 9
Component Identification 10
Vacuum Pump Connection 11
Electrical Connection 12
Baffle Installation 12
Drying Chamber or Manifold Installation 13
Chemical Resistance of Freeze Dryer Components 14
Solvent Safety Precautions 16
CHAPTER 4: LYO-WORKS™OPERATING SYSTEM 17
Control Panel 18
Initial Power Up 18
Touch Screen Operation 19
Home screen 19
Main Menu Icons 19
Moving between Menu Screens 19
Function Indicator Bar 19
System Parameters Display 20

Original instructions
Operation Buttons (Auto, Collector, Vacuum) 21
Programs screen 21
Data Logging screen 23
Settings screen 24
Language 24
Time & Date 25
Units 25
Security 25
Alerts 26
Configure Email Settings 27
Maintenance 28
Vacuum Pump Settings 28
Refrigeration System Settings 29
Freeze Dryer Line Voltage Offset 29
Calibration 29
Display 30
Network 31
Sensors screen 32
Drying Accessory screen 32
End Point Detection screen 33
Help screen 34
Diagnostics 34
User’s Manual 35
About 35
CHAPTER 5: USING YOUR FREEZE DRYER 36
Operation Checklist 36
Vacuum Pump Ballast Setting 37
Operating the Freeze Dryer 37
Auto Start-Up 37
Manual Start-Up 37
Setting the Operating Vacuum Level 37
Pre-Freezing Samples 38
Adding Sample 39
Starting a Program 40
Shut Down 40
Defrosting 40
Alerts 41
Copy (export) a Data Log File 43
Download a Data Log File via Ethernet 43
Copy a Program 44
CHAPTER 6: MAINTAINING YOUR FREEZE DRYER 45
Service Safety Precautions 45
Routine Maintenance Schedule 45
CHAPTER 7: TROUBLESHOOTING 47
Vacuum Pump 47

Original instructions
Gaskets, Tubing, Connections, Sample Valves 48
System Components & Collector Chamber Isolation 50
Refrigeration Module Operation 52
APPENDIX A: FREEZE DRYER COMPONENTS 53
APPENDIX B: FREEZE DRYER DIMENSIONS 60
APPENDIX C: FREEZE DRYER SPECIFICATIONS 63
Electrical Specifications 63
Environmental Conditions 64
APPENDIX D: FREEZE DRYER ACCESSORIES 65

Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
CHAPTER 1
INTRODUCTION
Congratulations on your purchase of a Labconco FreeZone® Freeze Dry System,
which is designed for laboratory lyophilization procedures. The refrigerant used in
the refrigeration system is HCFC-free so it will not endanger the environment.
The unit is easy to install and maintain. Proper care and maintenance of this
product will result in many years of dependable service.
Intended Use
The Labconco Freeze Dryers described in this manual are intended for laboratory
use to facilitate the lyophilization process.
Freeze Dry Process
Freeze drying is an important process in sample preparation and for the preservation
and storage of biologicals, pharmaceuticals and foods. Of the various methods of
dehydration, freeze drying (lyophilization) is especially suited for substances that are
heat sensitive. Other than food processing (e.g., coffee, whole dinners), freeze drying
has been extensively used in the development of pharmaceuticals (e.g., antibiotics) and
preservation of biologicals (e.g., proteins, plasma, viruses and cell lines). The
nondestructive nature of this process has been demonstrated by the retention of
viability in freeze dried viruses and microorganisms.
Freeze drying is a process whereby water or other solvent is removed from frozen
material by converting the frozen water directly into vapor without the
intermediate formation of liquid water. The basis for this sublimation process
involves the absorption of heat by the frozen sample in order to vaporize the ice;
the use of a vacuum pump to enhance the removal of water vapor from the surface
of the sample; the transfer of water vapor to a collector; and the removal of heat by
the collector in order to condense the water vapor. In essence, the freeze dry
process is a balance between the heat absorbed by the sample to vaporize the
ice and the heat removed from the collector to convert the water vapor into ice.

Chapter 1: Introduction
Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
Freeze Dry Rates
The efficiency of the Freeze Drying process is dependent upon the surface area and
the thickness of the sample, the collector temperature and vacuum obtained, the
eutectic point and solute concentration of the sample. It is important to remember
these factors when trying to obtain efficient utilization of your Freeze Dry system.
A listing of selected materials and their approximate drying times are shown in
Table 1 for your reference.
SAFE TEMPERATURE AND DRYING TIMES
FOR SELECTED MATERIALS
Material
10mm Thick
Safe
Temperature
°C
Collector
Temperature
°C
Hours
(Approx.)
Milk
-5
-40
10
Urea
-7
-40
10
Blood Plasma
-10 to -25
-40
16
Serum
-25
-40
18
Vaccinia
-30 to -40
-50
22
Influenza Vaccine
-30
-50
24
Human Tissue
-30 to -40
-50
48
Vegetable Tissue
-50
-80
60
*Total sample quantities are contingent on various freeze dryer capacities.
Up to the point of overloading the system, the greater the surface area of the
sample, the faster the rate of freeze drying. By contrast, for a given surface area,
the thicker the sample the slower the rate of freeze drying. This is based on the
fact that the heat of sublimation is usually absorbed on one side of the frozen
sample and must travel through the frozen layer to vaporize water at the other
surface. In addition, as the sample is freeze dried, the water vapor must travel
through the layer of dried material. The thicker the sample, the greater the chance
that the dried layer may collapse which would cause an additional decrease in the
rate of freeze drying.
The surface area and thickness of the sample can usually be ignored when each
sample contains only a few milliliters. However, for larger volumes, the samples
should be shell frozen to maximize the surface area and minimize the thickness of
the sample. The volume of the freeze dry flask should be two to three times the
volume of the sample.
In order for lyophilization to occur, ice must be removed from the frozen sample
via sublimation. This is accomplished by the collector and the vacuum pump. The
collector, which should be at least 15 to 20°C colder than the eutectic temperature
(melting temperature) of the sample, traps vapor as ice. Since the vapor pressure
at the collector is lower than that of the sample, the flow of water vapor is from the
sample to the collector. Since this vapor diffusion process occurs very slowly
under normal atmospheric conditions, a good vacuum is essential to maintain an
Table 1

Chapter 1: Introduction
Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
efficient rate. In many applications, the maintenance of a vacuum of 0.133 mbar
or less is recommended.
The rate of freeze drying is directly proportional to the vapor pressure and the
vapor pressure is dependent upon both eutectic temperature and solute
concentration of the sample. For example, a solution of sodium chloride and water
would freeze dry at a slower rate than pure water. The eutectic temperature of a
sodium chloride solution is about –21°C and at this temperature the vapor pressure
is about 1/16 that of water at 0°C. Although the eutectic temperature is not
dependent upon the concentration of sodium chloride, the vapor pressure of the
water would decrease as the concentration of sodium chloride increased. This is
due to the fact that as the solute concentration increases, less of the surface area of
the frozen sample is occupied by water. In general, most solutions or biological
samples will have a eutectic temperature of –10 to –25°C. However, if the sample
contains a simple sugar such as glucose or if the sample is animal or plant tissue,
the eutectic temperature may be as low as –30° to –50°C.
Freeze Dry Capacity
The volume of a sample that can be freeze dried at one time is related to factors
discussed previously and the size and design of the freeze dry system. With any
given instrument, the capacity is based on the surface area of the sample; the
eutectic temperature and concentration of the sample; and the rate and amount of
heat transferred to the frozen sample. Of these factors, the eutectic temperature is
the most important factor in determining the amount of sample that can be freeze
dried at one time, particularly when flasks are used. This is because as the eutectic
temperature decreases, the vapor pressure decreases but the rate of heat absorption
by the sample does not change. This tends to promote melting of the sample,
which leads to a marked increase in vapor pressure and ultimately overloads the
collector and vacuum pump. Samples that have eutectic temperatures of –20°C or
lower should be placed on the freeze dry system one flask at a time so that the
vacuum in the system may recover before adding another sample to the system. If
the vacuum does not recover, the capacity of the freeze dry system has been
exceeded and the sample should be removed.
If there is a problem with a particular type of sample melting when placed on the
freeze dry system, dilution of the sample with more water or providing some
insulation around the flask to decrease the rate of heat absorption by the sample
may help. If the eutectic temperature of the sample is –40 to –60°C, the freeze dry
system selected for use must be equipped with cascade type refrigeration so that
the collector temperature can be cooled to below –75°C, or a dry ice/solvent trap
may be used between the collector and the vacuum pump.

Chapter 1: Introduction
Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
Samples Containing Volatile Substances
In certain cases the solvent in a sample to be freeze dried may contain volatile
components such as acetonitrile, methanol, acetic acid, formic acid or pyridine. In
addition to these substances having an effect on the eutectic temperature, they may
increase the vapor pressure at the surface of the sample. Also, compared to water,
they will require the absorption of less heat for sublimation to occur. Hence,
samples that contain volatile substances will have a greater tendency to melt,
particularly when placed in flasks or exposed to room temperature. If a sample
containing a volatile substance tends to melt when placed on a freeze dry system,
dilution of the sample with more water will help keep the sample frozen. For
example, a 0.2M solution of acetic acid is much easier to freeze dry than a 0.5M
solution.
About This Manual
This manual is designed to help you learn how to install, use, and maintain your
Freeze Dryer. Instructions for performing routine maintenance and making minor
modifications to your Freeze Dryer are also included.
Chapter 1: Introduction provides a brief overview of the Freeze Dryer, explains
the organization of the manual, and defines the typographical conventions used in
the manual.
Chapter 2: Prerequisites explains what you need to do to prepare your site before
you install your Freeze Dryer. Electrical requirements are discussed.
Chapter 3: Getting Started contains the information you need to properly unpack,
inspect and install your Freeze Dryer.
Chapter 4: Lyo-Works Operating System describes the display screens and user
interface software that controls the Freeze Dryer.
Chapter 5: Using Your Freeze Dryer discusses the basic operation of your Freeze
Dryer. Information on how to attach samples and run the Freeze Dryer is included.
Chapter 6: Maintaining Your Freeze Dryer explains how to perform routine
maintenance on your Freeze Dryer.
Chapter 7: Troubleshooting contains a table of problems you may encounter while
using your Freeze Dryer, including the probable causes of the problems, and
suggested corrective actions.
Chapter 8: Modifying Your Freeze Dryer Display explains how to recalibrate your
Freeze Dryer display.
Appendix A: Freeze Dryer Components contains labeled diagrams of the
components of the Freeze Dryer.

Chapter 1: Introduction
Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
Appendix B: Freeze Dryer Dimensions contains diagrams showing the dimensions
for the Freeze Dryer.
Appendix C: Freeze Dryer Specifications contains product specifications.
Appendix D: Freeze Dryer Accessories see www.labconco.com for a complete list
of Freeze Dry accessories.
Typographical Conventions
Recognizing the following typographical conventions will help you understand and
use this manual:
Book, chapter, and section titles are shown in italic type (e.g., Chapter 3:
Getting Started).
Steps required to perform a task are presented in a numbered format.
Comments located in the margins provide suggestions, reminders, and
references.
Critical information is presented in boldface type in paragraphs that are
preceded by the exclamation icon. Failure to comply with the information
following an exclamation icon may result in injury to the user or permanent
damage to your Freeze Dryer.
Important information is presented in capitalized type in paragraphs that are
preceded by the pointer icon. It is imperative that the information contained in
these paragraphs be thoroughly read and understood by the user.

!

Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
CHAPTER 2
PREREQUISITES
Before you install your Freeze Dryer, you need to prepare your site for installation.
Carefully examine the location where you intend to install your Freeze Dryer. You
must be certain that the area is level and of solid construction. An electrical source
must be located near the installation site.
Carefully read this chapter to learn:
Electrical supply requirements.
Vacuum pump requirements.
Refer to Appendix C: Freeze Dryer Specifications for complete Freeze Dryer
electrical and environmental conditions, specifications and requirements.
Electrical Requirements
115V models
o20 Amp dedicated single phase circuit.
oProperly grounded receptacle for
NEMA 5-20P plug.
230V models
o15 Amp dedicated single phase circuit.
oProperly grounded receptacle that matches the plug supplied with
the unit, (230V models are supplied with one of the following
plugs):
US EU China UK India
(NEMA 6-15P)
US
NEMA 5-20P

Chapter 2: Prerequisites
Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
oIf the plug does not match the available receptacle, remove the plug
and replace it with an approved plug of the suitable style.
Do not use any detachable power cord that is not adequately
rated for the unit.
Frequency must agree with the serial tag rating.
Improper frequency will damage the compressor.
ADDITIONAL APPLIANCES ON THE SAME CIRCUIT MAY
CAUSE THE CURRENT TO EXCEED THE RATING OF THE
CIRCUIT BREAKER OR FUSE.
Location Requirements
The Freeze Dryer should be located in an area that provides an unobstructed flow
of air around the cabinet. This air cools the refrigeration system. The refrigeration
system draws air through the rear panel and exhausts it through the sides. A
minimum of 3" must be allowed between the back and both sides of the Freeze
Dryer and adjacent wall surfaces. Restriction of airflow during operation could
adversely affect performance.
Refer to Appendix B: Freeze Dryer Dimensions for dimensional drawings of the
Freeze Dryer. The space for benchtop models must be large enough to
accommodate the vacuum pump, which will be used with the Freeze Dryer.
Vacuum Pump Requirements
A vacuum pump must be provided by the user. A vacuum pump with a
displacement of 86 liters per minute and 0.002 mbar ultimate pressure is adequate
for most samples. The inlet fitting on the vacuum pump must be suitable for 3/4"
ID vacuum hose, which is provided. It is recommended that the vacuum pump is
equipped with an exhaust filter to minimize oil mist exhausting from the vacuum
pump. The operating vacuum level may be set on the Freeze Dryer. The higher
the vacuum set point, the more likely it is that oil mist will be exhausted.
Vacuum pumps used with 115V models should be equipped with a 115V, 15 Amp
NEMA 5-15P plug. Vacuum pumps used with 230V models should be equipped
with a reverse IEC plug (included with 230V pumps purchased from Labconco).
This will allow the vacuum pump to be plugged into the receptacle on the back
panel of the freeze dryer.
Chamber or Manifold Requirements
A freeze drying chamber or manifold is not included and must be purchased
separately. These accessories allow samples to be attached to the Freeze Dryer.
See Chapter 3: Getting Started for installation instruction.

!
!

Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
CHAPTER 3
GETTING STARTED
Now that the site for your Freeze Dryer is properly prepared, you are ready to
unpack, inspect, install and test your Freeze Dryer. Read this chapter to learn how
to:
Unpack and move your Freeze Dryer.
Set up your Freeze Dryer.
Connect the electrical supply source to your Freeze Dryer.
Properly exhaust your Freeze Dryer.
Safely use solvents with your Freeze Dryer.
The FreeZone Benchtop Freeze Dryers weigh between 82 & 142
lbs (37-64 kg) depending on the model. If you must lift the
Freeze Dryer manually, use at least two (2) persons and follow
safe lifting guidelines.
Unpacking Your Freeze Dryer
Carefully unpack your Freeze Dryer and inspect it for damage that may have
occurred in transit. If your Freeze Dryer is damaged, notify the delivery carrier
immediately and retain the entire shipment intact for inspection by the carrier.
DO NOT RETURN GOODS WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO. UNAUTHORIZED
RETURNS WILL NOT BE ACCEPTED.
!
The United
States
Interstate
Commerce
Commission
rules require
that claims be
filed with the
delivery carrier
within fifteen
(15) days of
delivery.


Chapter 3: Getting Started
Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
IF YOUR FREEZE DRYER WAS DAMAGED IN TRANSIT,
YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT
CARRIER. LABCONCO CORPORATION AND ITS DEALERS
ARE NOT RESPONSIBLE FOR SHIPPING DAMAGE.
DO NOT DISCARD THE CARTON OR PACKING MATERIAL
FOR YOUR FREEZE DRYER UNTIL YOU HAVE CHECKED
ALL OF THE COMPONENTS AND INSTALLED AND TESTED
THE FREEZE DRYER.
Freeze Dryer Components
Verify that the components listed below are present and undamaged.
(1) Freeze Dryer
(1) Collector Chamber Lid Assembly
oAcrylic Lid Assembly for -50C models
oStainless Steel Lid Assembly for -84C & -105C models
2.5L
4.5L
8L
Part #
Description
Comments
1
1
1
1089005
User’s Manual
USB Flash Drive
1
1
1
Power Cord
See parts list (Appendix A)
1
1
1
7373450
Vacuum Hose
2
2
2
1488800
Hose Clamp
1
7419300
Baffle
-84C Models Only
1
7208100
Baffle
-84C & -105C Models Only
If you did not receive one or more of the components listed for your Freeze Dryer,
or if any of the components are damaged, contact Labconco Corporation
immediately for further instructions.
Setting Up Your Freeze Dryer
After you verify receipt of the proper components, move your Freeze Dryer to the
location where you want to install it. Then, follow the steps listed below.



Chapter 3: Getting Started
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Original instructions
Component Identification
USB Port
Quick-Connect
drain fitting
Power Inlet
Vacuum Pump
Outlet
Quick-Connect
drain coupling
Circuit Breaker
Reset
Vacuum Connection
Port
Drying Accessory
End Point Detection
Ethernet
Touch Screen Display
On/Standby Button
& LED Indicator
Serial Tag
(& Electrical Rating)
Collector Chamber
& Coil

Chapter 3: Getting Started
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Original instructions
Vacuum Pump Connection
A vacuum pump (as described in Chapter 2: Prerequisites) is required to operate
your Freeze Dryer properly. The Freeze Dryer is equipped with a 3/4" ID vacuum
hose for connecting the collector chamber to the vacuum pump.
Make certain that the oil in the vacuum pump is at the proper
level.
1. Place the vacuum pump near the benchtop Freeze
Dryer.
2. Remove protective caps from inlet and outlet
ports.
3. Some vacuum pumps have inlet fittings that clamp
to the pump. Place the centering ring with filter
screen on the inlet fitting.
4. Place the hose barb fitting on top of the centering
ring and secure with the clamp.
5. CHECK THE VACUUM PUMP OIL LEVEL.
Fill the pump with oil so the level is between
the lines on the sight gauge on the end of the
pump housing.
6. Attach one end of the vacuum hose to the port
on the back of the Freeze Dryer and the other
end to the inlet port of the vacuum pump with
the hose clamps provided. If necessary, cut
the hose to proper length to allow for gentle
bends without kinks.
!
Hose Barb
Clamp
Inlet
Fitting

Chapter 3: Getting Started
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Original instructions
7. VERIFY THE VACUUM PUMP VOLTAGE SETTING AGREES WITH
THE FREEZE DRYER VOLTAGE SHOWN ON THE SERIAL
NUMBER LABEL. Reconfigure if necessary (see vacuum pump
instructions).
8. Connect the vacuum pump power cord to the receptacle labeled “vacuum
pump” on the back of the Freeze Dryer. Models rated for use on 230V
have a reverse IEC receptacle for attaching the vacuum pump power cord.
9. If the vacuum pump has an ON/OFF switch, turn the switch ON. The
vacuum pump will be controlled by the Freeze Dryer.
IT IS HIGHLY RECOMMENDED THAT AN OIL MIST
EXHAUST FILTER BE INSTALLED ON ROTORY VANE
VACUUM PUMPS TO MINIMIZE OIL MIST EXITING THE
PUMP. AN OIL RETURN KIT CAN ALSO BE USED IN
CONJUNCTION WITH THE EXHAUST FILTER TO RETURN
TRAPPED OIL BACK TO THE VACUUM PUMP.
Electrical Connection
Make sure that the outlet that you intend to use meets the voltage and amperage
requirements listed on the serial tag of the Freeze Dryer. Plug the power cord into
the receptacle on the back of the Freeze Dryer and plug the other end into a
suitable power outlet. It will take approximately 90 seconds for the operating
system to fully load.
Baffle Installation
A cylindrical plastic baffle is shipped with -84C & -105C Freeze Dryers (Models
71020xxxx, 71040xxxx & 72040xxxx). The baffle is designed to evenly distribute
collected ice over the entire collector coil. Proper positioning of the baffle is
required to achieve maximum ice loading capabilities.
The baffle installs into the collector chamber with the flat flange upward. The
arrow should point toward the front of the Freeze Dryer (as shown below).


Chapter 3: Getting Started
Product Service 816-333-8811 or 1-800-522-7658
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Original instructions
Drying Chamber or Drying Manifold Installation
A drying chamber or drying manifold (purchased separately) may be positioned
directly above the 3-inch hole in the collector chamber lid.
12 or 16 Port Drying Chamber Installation on Acrylic & Stainless Steel Lids
GASKET
STAINLESS STEEL
LID ASSEMBLY
DRYING CHAMBER
GASKET
COLLAR
LATCH
SPACER(SSTL)
SPACER(PLASTIC)
ACRYLIC
LID ASSEMBLY
DRYING CHAMBER
LID
GASKET LIP
FACING DOWN
Arrow pointing forward

Chapter 3: Getting Started
Product Service 816-333-8811 or 1-800-522-7658
14
Original instructions
4 or 8 Port Manifold Installation on Acrylic or Stainless Steel Lids
Clear Drying chambers come with their own bottom adapter plate that will be used
in place of the collector chamber lid supplied with the Freeze Dryer.
Clear Chamber Installation
Chemical Resistance of Freeze Dryer
Components
The FreeZone Freeze Dry System is designed to be chemically resistant to most
compounds that are commonly used in freeze drying processes. However, by
necessity, the freeze dryer is comprised of a number of different materials, some of
which may be attacked and degraded by certain chemicals. The degree of
degradation is dependent on the concentration and exposure duration. Some of the
LATCH
ACRYLIC LID
ASSEMBLY
GASKET
SPACER(SSTL)
MANIFOLD
SPACER(PLASTIC)
GASKET
STAINLESS STEEL
LID ASSEMBLY
GASKET LIP
FACING DOWN
GASKET LIP
FACING DOWN
CLEAR CHAMBER

Chapter 3: Getting Started
Product Service 816-333-8811 or 1-800-522-7658
15
Original instructions
major components of the FreeZone Freeze Dry System that are susceptible to
degradation are as follows:
Acids
Buffers
Solvents
Component
Material
Acetic Acid 20%
Formic Acid
Trifluoroacetic Acid (TFA)
Calcium Chloride
Sodium Phosphate
Acetone
Acetonittirle
Carbon Tetrochloride
Cyclohexane
Dioxane
Methyl t-Butyl Ether (BTBE)
Pyridine
Valve Stem
Acetal
C
D
D
D
D
Collector
Lid
Acrylic
D
D
D
D
Hoses,
Gaskets &
Valve
Bodies
Neoprene
C
D
D
C
C
D
D
D
C
D
Flask Top
Silicon
Rubber
C
D
D
D
D
D
C
D
Chamber &
Fittings
Stainless
Steel
C
C –Moderate degradation; Limited use
D –Severe degradation; infrequent use recommended; immediate thorough
cleaning required.
Most common compounds used in freeze drying processes, if allowed to enter
the vacuum pump, will degrade the oil and cause damage to the vacuum pump.
Sugars and proteins typically will have minimal negative effect on any of the
materials of construction.
When using compounds in the Freeze Dryer that are hostile to the materials of
construction, it is imperative the equipment is thoroughly cleaned after use.
Rubber and plastic components that have been exposed to damaging
compounds should be removed and flushed with water.
The oil in the vacuum pump should be checked often. It must be changed if it
is cloudy, shows particles or is discolored. The useful life of vacuum pump oil
can be extended if the vacuum pump is operated for an extended period of time
after a freeze dry run. This allows contaminants to be purged from the hot oil.
This must be done with the inlet to the pump blocked off to prevent air from
free flowing through the pump. If the pump is operated at an elevated vacuum
level (> 10mbar), oil may be expelled from the pump and damage could occur.