LI-COR LI-600N User manual

LI-600N Porometer/Fluorometer Quick Start Guide
Online Resources
Computer
Software Configuration and data management, calibration, barcode generator: licor.com/600Nsoftware
Support Manual, software, videos,FAQs, tech tips, application notes, and more: licor.com/600Nsupport
Instruction
Manual Comprehensive instructions, operating theory, and maintenance:licor.com/600Nmanual
1Chamber. Active measurement site
2Porometer. Measures stomatal conductance
3Fluorometer. Measures PAM chlorophyll afluorescence
4Enter button. Powers on, selects options, initiates or
logs a measurement
5USB port. Charges and connects to a computer with a
micro-USB cable
Charge the battery
The LI-600N arrives partially charged. Connect the LI-600N to a power source with the USB cable and charge to 100%.
Take a measurement
The following instructions are for the gsw+F configuration preloaded on the instrument.
Step 1: Press and hold the Enter button to power on the LI-600N.
Step 2: Select the preloaded gsw+F configuration and press the Enter button.
Step 3: The LI-600N will enter match mode. Respond to the prompt. When matched, it will beep and go to the Measurements screen.
Step 4: Clamp to a needle or leaf. Watch for stability in the gsw variable. Press the Enter button (Log) to log the measurement when gsw is
stable. After the LI-600N displays Logged and beeps, unclamp the needle or leaf. View the data from the measurement on the screen.
Step 5: Press the Enter button (New) to start another measurement, or press the left arrow twice to navigate to Settings to Shutdown.
Step 6: Download the data. Install the LI-600N computer software. Connect the LI-600N to the computer via USB and download the data to
the computer software. Export the data from the software as a .zip file that contains .csv and Excel files.

Workflow for taking a measurement
The workflow for taking a measurement varies depending on the configuration. The following is for gsw+F with prompt on log disabled. See the
user manual for detailed instructions on those settings.
2
1
Select a configuration
and press the Enter
button.
3
When finished, press
the left arrow twice,
select Shutdown and
press the Enter
button.
Select Configuration
gsw+F
gsw+F+prompt
---
---
gsw+F
Shutdown
Match
Instrument Settings
Settings
Warning(s)
Prompt
Log
Home
leak_pct Qamb
F
PhiPS2
flr2sec
gsw
gsw2sec
Tleaf
LI-600N
LI-600N
Cancel
RH_adj
Matching.
Do not clamp on leaf.
flow RH_delta
flow_s
!
LI-600N
Answer any prompts:
highlight the answer
and press the Enter
button.
4 5
Settings Info Home
Home New
LI-600N
Mark
leak_pct Qamb
F
PhiPS2
flr2sec
gsw
gsw2sec
Tleaf
Clamp onto a needle
or leaf, watch for
stability, then press
the Enter button (Log).
Press the Enter button
(New) to initiate
another measure-
ment, and repeat from
step 3,
Measurement techniques
lAlign the needle or leaf with the opening in the aperture. If they are not aligned, the IRT sensor will not accurately measure the
sample.
lMaintain the light conditions of the leaf. Do not alter the light or shade the leaf you are measuring. If, while taking a measurement,
you shade the leaf you plan to measure next, unshade the leaf and wait 60 seconds before measuring it. Changes to light at the leaf sur-
face will affect PhiPSII and gsw.
lMatch the quantum sensor's light conditions to those of the leaf. If the leaf you measure is in the light, ensure that the quantum
sensor is also in the light. If the leaf you measure is in shade, ensure that the quantum sensor is also in the shade. A mismatch between
the leaf and the quantum sensor may result in a significant error in ETRcalculation.
lAvoid measuring a needle or leaf with water on it. If the needle or leaf you plan to measure has water or condensation on it, gently
and thoroughly dry it. Measuring a wet needle or leaf may result in overestimation of stomatal conductance, and water ingress into the
instrument may cause damage.
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All rights reserved.
984-20614•10/2023
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