LightOx PhotoReact 365 User manual

Applies to PhotoReact 365 Model
lightox.co.uk
© LightOx Limited. 2020 All rights reserved
User Manual

Contents
Introduction
Safety
System Overview
Warnings
System Components
Choosing a Location
Connecting the Power Cord and USB cable
Setup
Setting the Date/Time
Performing an irradiation experiment
Re-running an experiment
Downloading experimental data
Clearing log files
Termination, Turning the Unit “Off”
System Information
Proper Care and Handling
Cleaning, Disinfection, and Disposal
Maintenance
Troubleshooting
Technical Data
Contact
1.
2.
3.
3.1.
4.1.
4.2.
4.3.
5.1.
5.2.
5.3.
5.4.
5.5.
5.5.1.
5.6.
6.
7.
7.1.
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No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system or
translated into any language in any form by any means without the prior written consent of LightOx Limited.
Every effort has been made to ensure information in this manual is accurate, however, information in this
manual is subject to change without notice and does not represent a commitment on the part of the authors.
LightOx Limited
65 Westgate Road
Newcastle upon Tyne, NE1 1SG
United Kingdom
Trademarks
LightOx® is a registered trademark of LightOx Limited.
All other product names are trademarks of their respective owners.
Designed in the United Kingdom; manufactured in Singapore
User Manual

The PhotoReact 365 is a bench top instrument for the acceleration
of photochemical and photobiological reactions
Instrument suitable for use with a standard size culture multi-well plate
(85.5 mm x 128 mm) or single 95 mm round dish
User selected light output from 0 mW/cm2to maximum (approx. 13.0 mW/cm2)
Variance less than 1 mW/cm2(at 100% power) and 0.5 mW/cm2
(at 50% power) across the well plate surface
User control of total power output
Light intensity and temperature monitors included that records
measurements every second during operation
Data export as .csv files
Device has locking lid that reduces user exposure to harmful light rays
Technical Specifications PhotoReact 365
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Key Features
The PhotoReact 365 is a benchtop
instrument that provides even, directed
and reproducible UV illumination
at 365 nm suitable for photochemical
and photobiological reactions.
1. Introduction
The user can control parameters of intensity and energy
delivery rate and time. LED health over time is monitored using
an in-built UV sensor enabling the user to produce rapid,
reproducible results. This instrument is suitable for
high-throughput screening in multiwell plates, providing
researchers with a tool to screen high numbers of compounds
and reactions essential for drug discovery.
User Manual
3

Prior to use, the operator should
thoroughly read the instructions for use.
Using this device without reading and
understanding the instructions for use
may result in operator injury or damage
to the equipment.
2. Safety
User Manual
The device contains powerful LEDs that produce bright
UV light. The device should only be used with the locking
lid in place. Do not bypass the lock activation switch
as looking into the device when activated may result
in damage to eyes or to exposed skin.
Only use the device with appropriate dishes or plates.
Proper care must be taken during setup and operation
to prevent injury to operators and other personnel or
damage to the unit.
The unit is equipped with multiple safety features
including a lock activation switch that will not permit
UV emission when the lid is not in place.
4

The PhotoReact 365 is a device that
can deliver a specific amount of light
to a sample plate in a controlled
environment. Controlling the power
(light intensity) and irradiation time
(seconds) enables specific energy
across the whole sample plate.
3. System Overview
User Manual
The PhotoReact 365 will provide an even illumination,
using light sources that allow for controlled intensity
and wavelengths to be delivered to the sample plate.
The PhotoReact 365 includes a UV sensor to monitor
the health and light output of the LEDs. LEDs will
diminish in power slowly over time and the inbuilt
sensor allows the user to monitor the functionality
of the LEDs in their instrument. The irradiance output
and UV sensor are factory calibrated. Re-calibration
of the system is possible. Contact the manufacturer
for further information.
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User Manual
The PhotoReact 365 produces UV light. Injury to the
eyes or skin can result from UV exposure. It is the
responsibility of the user to follow all applicable safety
guidelines in prevention of injury or damage to the
unit. Never bypass the lid locking switch.
No modification of this equipment is allowed.
Never open or remove the bottom cover
of the base unit or control unit.
The PhotoReact 365 unit requires adequate airflow to maintain proper
cooling. Ensure the ventilation slots on the side of the unit are unobstructed.
Not for domestic use. In accordance with EN 55011: 2009 +A1:2010
the PhotoReact 365 is a Class A product. In a domestic enviroment this
product may cause radio interference in which case the user may
be required to take adequate measures.
If the PhotoReact 365 is used in a manner not specified within this
manual, the protection provided by the equipment might be impaired.
It is the responsibility of the user to follow all applicable safety
guidelines in prevention of injury or damage to the unit.
It is the responsibility of the user to assess and mitigate any hazards
that may result from experimental reactions.
It is the responsibility of the user to assess and mitigate any hazards that
may result from addition of volatile or flammable materials. Users shall
comply with all applicable safety and accident-prevention procedures
for laboratory work.
Device intended for a variety of chemical and biological reactions;
Device to be used by trained operator in a laboratory setting.
Heavy – Product with its packaging is over 7 kg.
Appropriate manual handing guidelines should be followed.
Power supply cannot be replaced.
3.1. Warnings
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The PhotoReact 365 is comprised of the following:
Base Unit
Removable, locking lid
Control Unit
USB cable connecting Control Unit to Base Unit
Power Supply
User Manual
4. System Components
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User Manual
Place the PhotoReact 365 on a flat surface
on its neoprene feet with at least
15 cm of space between the perimeter
of the instrument and the nearest object.
The PhotoReact 365 is designed
to work at ambient room temperature.
Transport the PhotoReact 365
in a horizontal position and avoid
significant jarring of the instrument
whilst on the worktop.
4.2. Choosing a Location
15cm 15cm
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User Manual
Insert the one end of the USB cable from the USB port
on the right side of the Control Unit.
Insert the other end of the USB cable into the USB port
on the Base Unit.
Insert the power supply terminal into the power connector
port on the controller unit. Insert the power supply plug
into a standard mains AC outlet.
Warning: Risk of electrical shock
Before plugging into mains power visually inspect the power
supply plug and cable to ensure there is no damage.
If there are signs of damage do NOT use.
Note: There are two USB ports on the control unit. The right hand port should
be used to connect the USB cable on the control unit to the USB port on the
base unit. If the USB cable is incorrectly connected to the data port on the
back side of the control unit, the instrument will not function.
4.3. Connecting the Power Cord
and USB cable
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User Manual
Once the power cord and the USB cables are connected
the PhotoReact 365 Base Unit and Control Unit are ready
for operation.
The display start-up screen is shown below. The touch
screen buttons are New experiment, Rerun experiment
and the system Settings button.
New experiment allows the user to input new settings.
Rerun experiment allows the user to reuse settings
from a previously run experiment.
When setting up the PhotoReact 365 for the first time,
the time and date should be set by the user. Touch
the systems setting button on the start up screen.
Operation
5.1. Setup
New experiment Rerun experiment
Settings
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