REPLIGEN KrosFlo KTF User manual

1
IF.UG.028 R1
KrosFlo® Systems
User Guide
For use with:
•KrosFlo® KTF Tangential Flow Filtration (TFF) System
•KrosFlo® KPS Tangential Flow Filtration (TFF) System
•KrosFlo® TFDF® Tangential Flow Depth Filtration (TFDF) System

User Guide
User Guide
KrosFlo® Systems
2
IF.UG.028 R1
The information contained in this document is subject to change without notice.
With respect to documentation accompanying product, Repligen makes no warranty, express or
implied. Any and all warranties related to the documentation accompanying product are expressly
disclaimed. Customer shall refer to the terms and conditions of sale governing the transaction for
any and all warranties for the Product.
Repligen Corporation shall not be liable for errors contained herein or for incidental or
consequential damages in connection with the furnishing, performance, or use of this material.
No part of this document may be photocopied, reproduced, or translated to another language
without the prior written consent of Repligen Corporation.
Products are not intended for diagnostic or therapeutic use or for use in vivo with humans or
animals.
For further information, please contact Repligen Corporation at www.repligen.com.
©2020 Repligen Corporation. All rights reserved. The trademarks mentioned herein are the property
of Repligen Corporation and/or its affiliate(s) or their respective owners.
Customer Support
[e-mail]
508-845-3030
Repligen Corporation
111 Locke Drive
Marlborough, Massachusetts 01752
www.repligen.com

User Guide
User Guide
KrosFlo® Systems
3
IF.UG.028 R1
Contents
7.8.1 Warning alarms...........................................................................................................20

User Guide
User Guide
KrosFlo® Systems
4
IF.UG.028 R1
List of tables
Table 1. Signal words, definitions and colors .....................................................................................6
Table 2. Safety symbol icons ..............................................................................................................7
Table 3. KrosFlo® Systems specifications ...........................................................................................9
Table 4. Input parameters for Main screen......................................................................................15
Table 5. Input parameters for Settings screen.................................................................................18
Table 6. Alarm Setup screen setpoint range ....................................................................................19
Table 7. Chart screen parameters ....................................................................................................21
Table 8. System fuses .......................................................................................................................24
Table 9. Installed major components and identification tags..........................................................24
Table 10. Recommended spare parts ................................................................................................25
List of figures
Figure 1. Caution and warning examples ...........................................................................................8
Figure 2. Polysulfone pressure sensors example .............................................................................11
Figure 3. Installed flow path pressure sensor ferrite bead ..............................................................12
Figure 4. Main screen example ........................................................................................................14
Figure 5. Settings screen (Fed Batch disabled).................................................................................16
Figure 6. Tubing Selection popup screen .........................................................................................17
Figure 7. Alarm Setup screen example.............................................................................................19
Figure 8. Alarms screen example .....................................................................................................20
Figure 9. Chart screen example........................................................................................................21
Figure 10. Report screen example .....................................................................................................22

User Guide
User Guide
KrosFlo® Systems
5
IF.UG.028 R1
Abbreviations
Amp Ampere
C Celsius
CF Concentration factor
cm Centimeter
DV Diafiltration volume
EU Europe
F Fahrenheit
ft Feet
HMI Human machine interface
Kg Kilograms
TFDF Tangential Depth Filtration System
Lbs Pounds
LCD Liquid-crystal display
lpm Liters per minute
M Meter
mA Milliampere, or milliamp
MBT Module bag tubing
NWP Normalized Water Permeability
PLC Programmable logic controller
Psi Pounds per square inch
rpm Revolutions per minute
SAS Standard alert symbol
TFF Tangential Flow Filtration
TMP Transmembrane pressure
VAC Volts AC power

KrosFlo® Systems
User Guide
6
repligen.com IF.UG.028 R1
1. Introduction
KrosFlo® Systems from Repligen provide ready-to-use, flexible flow path solutions for commercial-
scale microfiltration and ultrafiltration process operations. The systems include one or two
magnetically driven centrifugal pump heads, custom ProConnex® Flow Paths, flow meters, peristaltic
pumps, Spectrum® Hollow Fiber Filters, KrosFlo® TFDF® Filters and optional connections for a weight
scale for additional process monitoring.
Other components of the system include the retentate flow meters, permeate flow meter, pressure
sensors, Repligen control panel controlled by an Allen-Bradley PLC, using an iFIX-based HMI program
to initiate actions, flexible tube flow path, and a Repligen stainless steel frame/cart that serve as the
system support structure.
2. Safety precautions
2.1 Signal words
The signal word and color distinguish the severity level of a hazard. Signal word definitions and
colors are listed in Table 1.
Table 1. Signal words, definitions and colors
2.2 Safety symbol icons
The typical safety symbol icons - hazard, prohibition, and mandatory action - used on Repligen
systems are listed in Table 2. The icons are pictograms that communicate hazards quickly and across
language barriers.
Description
Safety Alert Symbol (SAS) - used when a hazard to personnel is present. The SAS is
omitted when the hazard is related to property/equipment damage only.
A DANGER notification indicates an imminently hazardous situation which, if not
avoided, will result in death or serious injury.
A WARNING notification indicates a potentially hazardous situation which, if not
avoided, could result in death or serious injury.
A CAUTION notification indicates a potentially hazardous situation which, if not avoided,
may result in minor or moderate injury.
A CAUTION notification without the Safety Alert Symbol indicates a potentially
hazardous situation which, if not avoided, may result in property/equipment damage.

KrosFlo® Systems
User Guide
7
repligen.com IF.UG.028 R1
Table 2. Safety symbol icons
Electrical Hazard Heavy Object Heat Hazard
Radiation Hazard
Crush Hazard
Pinch Hazard
Inhalation Hazard
Authorized and Qualified
Personnel Only
Read the Manual
Noise Level Hazard

KrosFlo® Systems
User Guide
8
repligen.com IF.UG.028 R1
3. Safety guidelines
To ensure safe operation:
1. Do not operate the system without first reading this manual.
2. Read the following caution and warning statements:
Figure 1. Caution and warning examples
WARNING –To avoid risk of electric shock, this equipment must only
be connected to a supply main with protective earth. No
modification of this equipment is allowed.
WARNING –Do not plug in the system during Mechanical
Assembly.
WARNING –Do not plug the system in during Electrical
Connections procedure until instructed.
WARNING –System must be turned off when replacing fuses to prevent
electrical hazard. Any electrical work beyond the electrical
connections procedure and fuse replacement should be
performed by trained personnel only.
WARNING –Peristaltic pumps must be turned off when replacing either
tubing or pump heads to prevent crushing/pinching hazard.
WARNING –Due to a noise level of 80 decibels or greater, ear
protection is required when the System pumps are
operated at a speed of greater than (>) 6000 RPM.

KrosFlo® Systems
User Guide
9
repligen.com IF.UG.028 R1
4. System specifications
Table 3. KrosFlo® Systems specifications
5. Major components
The major components used in KrosFlo® Systems are:
•Retentate (recirculating) Pump (P-01) - Levitronix LPM-600.3 - 10 low-shear centrifugal
pump
•Product pump (P-02) - MasterFlex® 77420-10 I/P peristaltic pump
•Buffer Pump (P-03) - MasterFlex® 77420-10 I/P peristaltic pump
•Retentate flowmeter - Sonotec® FS04.210 C0.55/230 V2.0
•Permeate flowmeter - Levitronix LFS-06SU / LFS-10SU
•Reactor scale input - 4 - 20 mA
•Pressure sensors - Repligen disposable pressure transducers
•Controller - Allen-Bradley PLC (Programmable Logic Controller)
•HMI - Allen-Bradley Integrated Panel Display Computer (Human-Machine Interface)
•ProConnex® Flow Path - Designed flexible tube flow path from Repligen
•Support structure - Stainless steel filter stand and lab cart from Repligen
Weight
300 lb. (approximately)
135 Kg
500 lb. (approximately)
225 Kg
Dimensions
(L x W x H)
32 x 24 x 52.5 inches
81 x 61 x 133 cm
47 x 24 x 80 inches
120 x 61 x 203 cm
(with extension)
Power requirements
200-240 VAC 1ph 10A (EU)
200-240 VAC 1ph 15A
Outputs
Pumps:
P-01 100 - 9000 RPM
P-02 0.1 - 650 RPM
P-03 0.1 - 650 RPM
Display:
15” LCD touchscreen
Environmental
Electrical cabinet
and HMI – IP66
Operating temperature:
0 - 40° C
32 - 104° F
Chemical resistance
Frame and electrical cabinet:
Stainless Steel-304
Casters:
Stainless Steel-304 and
Polyurethane
ProConnex® Flow
path components:
Polypropylene,
polycarbonate,
Polysulfone, and
C-Flex®/
Pharmapure®
materials

KrosFlo® Systems
User Guide
10
repligen.com IF.UG.028 R1
6. Instructions for use
6.1 Installation and mechanical assembly
KrosFlo® Systems have a small footprint and require only a small clean area (approx. 3 m x 3 m/10 ft.
x 10 ft.) for assembly. No tools are needed for system assembly, though some basic hand tools such
as screw gun, pliers, and screwdrivers are needed to uncrate the system.
Some of the major components listed above are mounted on the cart. The other components can be
removed from boxes and installed on the cart for use in the process.
Follow these high-level steps to install and assemble the system:
1. Uncrate the system and remove all packaging material from the system components.
2. Locate and attach the proper filter brackets to the frame. The filter stand extension is
attached to the cart frame by attaching it to the filter post. Be sure to place a black rubber
gasket between the extension and frame post. Secure the extension with the provided metal
clamp and hand tighten, or with a wrench.
3. Place the Product Pump (P-02) on the lower cart frame. Connect the power and control
cables.
4. Place the Buffer Pump (P-03) on the cart frame. Connect the power and control cables.
5. The Recirculating Pump (P-01) will come already mounted. Consult the layout diagram
provided in the documentation package.
6. Install and connect scale (WE-01), or scale interface cable. If the tanks are more than one,
accordingly connect the scales as WE-02, WE-03 etc.
7. Route and connect all cables. Cables are marked based on their destination. All cables
should be routed to minimize kinking and obstructions.
8. Once the filter stand is assembled and pump motor is mounted, ready the pump head and
flow path/MBT assembly items. Make sure all necessary components to complete the
process line are available and ready (reactor, tubing, welding equipment, etc.). Please note
that the process assembly should be designed to minimize hold-up volume.
9. To prepare the pump head and flow path/MBT assembly:
a. Carefully support the MBT/filter/pump head assembly and hold at an angle such
that the pump head discharge lines up with the green color band at the pump
support on the end of the motor drive.
b. Install the pump head into the pump motor drive making sure to disengage/pull out
the locking pin to fully seat the pump head.
c. Rotate the pump discharge port and filter assembly to the vertical position and
towards the red color band on the pump drive. The locking pin will click into place
when the pump head is properly oriented in the pump drive.
d. Locate the filter in the 2 filter clamps on the frame post, making sure the clamps
attach to the filter on the clear section of the housing (not on end fittings). Inspect
the filter and pump before tightening clamps in place; there should be no strain
placed upon any of the filter, pressure sensor or pump connections.
WARNING –Do not plug in the system during Mechanical
Assembly.

KrosFlo® Systems
User Guide
11
repligen.com IF.UG.028 R1
10. With filter and pump securely in place, install the permeate tubing, making sure there is no
bending or kinking of the permeate tubing. Run extra tubing to the permeate vessel. Make
sure permeate pressure sensor (PE-03) is located between filter housing permeate port and
any valves prior to permeate collection.
11. Locate the clamp-on retentate flowmeter (FL-01) on the filter stand. Install the retentate
tubing in the flowmeter and close the flowmeter clamp. Make sure there is 10 - 15 cm (4” -
6”) of straight run tubing on each side of the meter. The return tubing may need auxiliary
support to prevent kinking. Clamp the meter onto tubing. Note flow direction arrow on
meter should point in up direction.
6.2 Electrical installation
All electrical connections are made with plugs and sockets located on the rear side of the control
panel.
Power requirements for system
200 - 240 VAC, 10/15 Amp Service, single phase.
To complete the system electrical connections:
1. Connect P-01, P-02, and P-03 connectors to panel.
2. Connect flow meter FL-01, FL-02 connectors to the flow meters.
3. Connect three pressure sensor cables (PE-01 feed, PE-02 retentate, PE-03 permeate) to the
flow patch pressure sensors.
4. Connect Main Panel power cord to suitable electrical outlet (200 - 240VAC, 10/15 Amp
Service).
5. Make sure that the Emergency Stop (E-Stop) button is pulled to OUT (inactive) position.
6. Turn on the Main Power by rotating the handle on the front of the control cabinet; this
starts the computer, and the control software loads automatically.
7. Press Reset button (Blue) on Control Panel.
6.3 Ferrite Bead installation
Polysulfone pressure sensors/transmitters are included as part of the sterile disposable ProConnex®
Flow Path (Module Bag Tubing (MBT)) that is shipped with your KrosFlo® KTF System.
Figure 2. Polysulfone pressure sensors example
A ferrite bead (P/N 3000541) is provided for each flow path pressure sensor to protect it against
possible electromagnetic interference (EMI). We recommend that you install the supplied ferrite
bead on each flow path pressure sensor cable to provide EMI protection for the pressure sensor.
WARNING –Do not plug in the system during Electrical Assembly
until instructed.

KrosFlo® Systems
User Guide
12
repligen.com IF.UG.028 R1
Figure 3. Installed flow path pressure sensor ferrite bead
To install the supplied flow path pressure sensor ferrite bead:
1. Locate the ferrite bead as close as possible to the flow path pressure sensor while ensuring
there is enough cable to wrap around the ferrite bead.
2. Open the ferrite bead.
3. Seat the flow path pressure sensor cable in the bead.
4. Wrap the cable around the bead.
5. Snap the bead closed.

KrosFlo® Systems
User Guide
13
repligen.com IF.UG.028 R1
7. KrosFlo® System operation
7.1 System overview
KrosFlo® Operating Systems are an iFIX-based program installed on a touchscreen HMI PC. The
program consists of six primary screens – Main (Operational), Settings, Chart (Process Trending),
Alarm Setup, Alarm, and Report. Screen navigation is performed using the buttons on the bottom of
each screen. Simply touch the button to go to the desired screen.
To end a session, navigate to the Settings Screen. Touch the Exit iFIX button located at lower right of
screen, then close the iFIX program before turning off computer.
The HMI screen will go into screen saver mode while the system is operating or while program is
open, just as a typical PC during times of inactivity. This will not affect operation and touching the
screen will display the present active screen.
7.2 Getting started
KrosFlo® Systems are ready to run after all Mechanical and Electrical Installation steps have been
completed.
To run KrosFlo® Systems:
1. Pull the E-Stop button (located on front of Control Panel) to the OUT position.
2. Turn the front-panel power switch to ON position.
3. Press the BLUE reset button on control panel.
Once the computer is booted up, use the touchscreen to navigate to the TFF icon on the screen.
Double click on the TFF icon to enter the KrosFlo® Operating System. The control system will open to
the Introductory screen, which contains the system part number and Installed Software Rev
information. Touch this screen anywhere to navigate to the Main screen.
At startup, KrosFlo® Systems will display all alarms as being active; they should be cleared before
operating the KrosFlo® System. From the Main screen, navigate to the Alarms screen. Once at the
Alarms screen, touch the Reset Alarm button on the touchscreen then touch the Ack Alarms button.
All Alarms will be cleared.
NOTE: The BLUE Reset button on the control panel enclosure is pressed to reset the Master Control
Relay at startup, power outage, or E-Stop only. All other alarm conditions are reset from the
Alarms screen on PC/HMI.
Be sure to exit iFix, and shutdown Windows before shutting down the KrosFlo® System.

KrosFlo® Systems
User Guide
14
repligen.com IF.UG.028 R1
7.3 Main screen
Figure 4. Main screen example
The Main screen displays the KrosFlo® Systems operational flow path. The system operation and
control features can be observed on the Main screen. Process data (flow, pressure, volume) is
displayed on screen in real time. Process data OUTPUTS are displayed in the YELLOW highlighted
boxes. Setpoint input data is displayed in the BLUE highlighted boxes.
Auto and Manual modes of operation for the Recirculating and Product Pumps are selected here.
The system can operate independently (outside of an automated process sequence), allowing the
user greater flexibility in performing trials to optimize filter performance prior to entering a run.
Setpoint data is entered by touching a BLUE input box. Type in the new Setpoint data and press
ENTER (= Equal Sign) button on the pop-up keyboard. The ENTER (= Equal) button MUST be pressed
after data entry or the new Setpoint value will not be stored.

KrosFlo® Systems
User Guide
15
repligen.com IF.UG.028 R1
Table 4. Input parameters for Main screen
7.4 Automatic (Process) Mode
KrosFlo® Systems are designed with an automated Process Sequence that controls a product
concentration step followed by a diafiltration (wash) step and then a final concentration step. The
process mode includes an option for use of Fed Batch mode.
The CONCENTRATION steps allow the system to run at a constant Transmembrane Pressure (TMP)
and recirculation flow (P-01 LPM) while concentrating the initial product sample volume to a
predetermined volume reduction. The volume reduction, or Concentration Factor (CF) is determined
by the user.
DIAFILTRATION sets the system to run at a constant Transmembrane Pressure (TMP) and
recirculation flow rate (P-01 LPM) while washing the concentrated sample product volume
(diafiltering) with buffer. The amount of wash (Diafiltration Volume, or DV) is determined by the
user. DV Setpoint is used as the end point of the diafiltration sequence. This number signifies the
number of diafiltration volumes that will be processed.
FED BATCH CONCENTRATION allows the initial concentration step to include additional volume from
a vessel external to the primary recirculation vessel being used. The calculations for process
estimate the remaining volume based on the permeate flow and scale reading for the main vessel.
When the main vessel weight reduces below the Level Setpoint During Concentration setpoint for
the time defined by the Sample Feed Pump Stop Delay setpoint, the KrosFlo® System will assume
the feed vessel is empty and switch its volume calculation to monitoring only the scale.
The FINAL CONCENTRATION step takes place once the diavolume setpoint has been reached for the
diafiltration step. The concentration factor is set on the process Settings screen and can be observed
from the Main screen.
The process steps are accessed on the Settings screen (see Section 7.6). A Reset Windup button is
available on the Settings Screen to reset PID control parameters after the system has been stopped.
This feature allows for a smoother restart of the system at auto process parameters. When the
Reset Windup button is not pressed, the control loop will start at the pump speeds previously
arrived at output.
Parameter Range Units
VALVE BPCV-01:
Manual Mode
Auto Mode
0 - 100
0 - 30
%
psi
Pumps:
P-01 Manual Mode
P-01 Auto Mode
P-02 Manual Mode
P-02 Auto Mode
P-03 Manual Mode
P-03 Auto Mode
0 - 9000
0 - 10/45/85
0 - 650
0 - 20
0 - 650
0 - 20
rpm
lpm
rpm
lpm
rpm
lpm
P-01, P-02 and P-03 may change depending on the system design, pump models and identification tags
used.

KrosFlo® Systems
User Guide
16
repligen.com IF.UG.028 R1
7.5 Manual Mode
KrosFlo® Systems can run in a manual mode which will operate the Back Pressure Control Valve (%
Closed), Permeate Pump (RPM), Feed Pump (RPM) and Recirculation Pump (RPM) at constant
settings with no process control.
The Manual mode button must be selected for each device (Pumps and Back Pressure Control Valve)
and appropriate setpoints selected. Setpoints are entered in the BLUE boxes. Once started, KrosFlo®
Systems will maintain setpoint control, however in Manual mode, it will use a constant pump speed
(RPM) or valve setting (% closed), and NOT a process variable control. Once started, KrosFlo®
Systems will maintain setpoint control during operation. Setpoints may be changed during TFF
process operations.
7.6 Settings screen
The Settings screen provides the settings for the Filter Model. Selecting a filter model will populate
the Fiber Count, Lumen ID, and Surface Area. These parameters are used for the calculation of shear
and flux. In the event a filter is not listed, the Fiber Count and Lumen ID must be entered in the BLUE
boxes shown in the Filter Model box to ensure correct shear rate data.
Figure 5. Settings screen (Fed Batch disabled)
The Settings screen also allows PID loop tuning of pump response for all pumps. The pump response
to startup and setpoint change can be tailored using PID tuning. Setpoint Data is entered as
described before: navigate to the appropriate Blue box and touch the setpoint box, type in data, and
press ENTER (= equal sign) button on the pop-up keyboard. All setpoint data should be entered prior
to operation. Press RESET PID TO FACTORY SETTINGS to return all pumps to their default PID tuning
values.
Press the Use Weight/Use CF toggle button to specify whether the Initial and Final Concentration
setpoints are based on the specified weight (Kg) or concentration factor.

KrosFlo® Systems
User Guide
17
repligen.com IF.UG.028 R1
Press the 0 - 20 lpm/0 -8 lpm toggle button to specify the range of the Permeate flow meter.
Press Tubing Selection to select the size of the tubing to be used with the KTF/KPS/TFDF® peristaltic
pump (Product Pump (P-02)). You may also enter a calibration factor for the selected tubing on this
popup screen.
Figure 6. Tubing Selection popup screen
Press the Fed Batch Enabled / Fed Batch Disabled toggle button to enable or disable fed batch
processing.
With Fed Batch disabled, you may press the Starting Vol = Enter Above/Starting Vol. = Use Scale
toggle button to specify whether the Starting Volume (that is, the volume in the feed tank at the
start of the concentration process) is entered as a setpoint or is based on the weight of the tank
(WE-01).
With Fed Batch enabled, the Level Setpoint During Concentration and Sample Feed Pump Stop
Delay setpoints are available for configuration.
The process setpoints that may be configured from the Settings screen are defined as follows:
•Starting Volume - the volume in the feed tank at the start of the concentration process. This
setting will be used to calculate the Concentration Factor, over time based on the remaining
volume after the start of the process.
•System Holdup Volume - used to compensate for the volume of liquid that is in the flow
path during the concentration process.
•Level Setpoint During Concentration - the level that the system will maintain in the feed
vessel while in fed batch concentration mode.
•Initial Concentration Setpoint - setpoint for the start of the Initial Concentration Sequence,
if run.
•Final Concentration Setpoint - setpoint for the end of the Final Concentration Sequence.
•Sample Feed Pump Stop Delay - the amount of time that the sample pump will run after the
weight on the feed tank drops below the setpoint volume.
•DV Setpoint - end point of the diafiltration sequence. This number signifies the number of
diafiltration volumes that will be processed.
•TMP Setpoint - Constant Transmembrane Pressure setting for TFF processes.
•Recirc Flow Setpoint - controls the speed of the recirculation pump (P-01).
•Feed Temp - used in calculating Normalized Water Permeability (NWP). A description of
NWP can be found in Section 12.5 at the end of this manual.
Table 5 lists the ranges of values for all input parameters on the Settings screen.

KrosFlo® Systems
User Guide
18
repligen.com IF.UG.028 R1
Table 5. Input parameters for Settings screen
NOTE: Values shown in the “Range” column are examples and may vary depending on the system
and process.
Press the Exit iFix button located on the lower right corner of the Settings screen to exit the
program.
Parameter Range Units
Process Setpoints:
Starting Volume
System Holdup Volume
Level Setpoint During Concentration
Initial Concentration Setpoint
Final Concentration Setpoint
Sample Feed Pump Stop Delay
DV Setpoint
TMP Setpoint
Recirc Flow Setpoint
Feed Temp
Batch ID
0 - 1600
0 - 100
0-600
0 - 600/0 - 100
0 - 600/0 - 100
0 - 300
0 - 100
0 - 30
0 - 85
0 - 100
Up to 20
liters
liters
liters
kg/X
kg/X
seconds
DV
psi
lpm
° C
characters
Parameter Range Units
Filter Model:
Filter Model Number
Fiber Count
Lumen ID
MWCO
Chemistry
Effective Length
Surface Area Entry
Qty of Modules
Up to 13
0 - 50,000
0 - 100
Up to 10
Up to 10
Up to 5
Up to 200,000
Up to 1
characters
fibers
lumen
characters
characters
characters
cm2
characters
PID setup Range Units
Recirculation Pump (P-01):
Recirculation Pump (P-01) P
Recirculation Pump (P-01) I
Recirculation Pump (P-01) D
0 - 1000
0 - 1000
0 - 1000
4
4
1
Product Pump (P-02):
Product Pump (P-02) P
Product Pump (P-02) I
Product Pump (P-02) D
0 - 1000
0 - 1000
0 - 1000
4
4
1
Buffer Pump (P-03):
Buffer Pump (P-03) P
Buffer Pump (P-03) I
Buffer Pump (P-03) D
0 - 1000
0 - 1000
0 - 1000
9
5
1

KrosFlo® Systems
User Guide
19
repligen.com IF.UG.028 R1
7.7 Alarm Setup screen
Figure 7. Alarm Setup screen example
The Alarm Setup screen contains 16 process alarm conditions to help protect the process from
unfavorable process conditions. 15 of the Alarms have the option of being Enabled or Disabled. The
one alarm condition which cannot be disabled is Feed High Pressure duration because it describes
how long an overpressure condition can exist without setting the shutdown alarm.
Table 6. Alarm Setup screen setpoint range
Alarm setpoints Range Units
Low Feed Pressure (PE-01)
High Feed Pressure (PE-01)
Feed High Pressure Shutdown (PE-01)
Feed High Pressure Duration
Low Retentate Pressure (PE-02)
High Retentate Pressure (PE-02)
Low Permeate Pressure (PE-03)
High Permeate Pressure (PE-03)
High Permeate Volume (FL-02)
Low Permeate Flow (FL-02)
High Permeate Flow (FL-02)
Low Recirc Flow (FL-01)
High Recirc Flow (FL-01)
High Feed Tank Level (FL-01)
High Shear
Low Shear
0 - 30
0 - 30
0 - 30
300
0 - 30
0 - 30
0 - 30
0 - 30
0 - 10000
0 - 20.0
0 - 20.0
0 - 85
0 - 85
0 - 200
0 - 12000
0 - 12000
psi
psi
psi
seconds
psi
psi
psi
psi
l
lpm
lpm
lpm
lpm
kg
sec -1
sec
-1

KrosFlo® Systems
User Guide
20
repligen.com IF.UG.028 R1
7.8 Alarm screen
Figure 8. Alarms screen example
This Alarms screen is for viewing, acknowledging, clearing, and resetting all Alarms. A list of all
Alarms, Alarm Range, and Tag (sensor or device responsible for Alarm Setpoint) is provided in Table
7.
Note: For the initial start-up (plugging in system to power), or in the event of power outage or
Emergency Stop, press the Blue Reset button on the control panel enclosure to reset alarms. All
other alarm conditions are reset from the Alarms screen.
The Ack Alarm button will acknowledge the alarm and clear it from the screen if the alarm is not
currently active.
The RESET ALARM button will silence the alarm and reset the flashing ALARM button once the
system is operating in the normal range.
Alarms can be enabled or disabled manually using the ALARM SETUP screen. To disable or prevent
an alarm, select the Disabled button. ‘Disabled’ should be displayed for alarm to be disabled.
Likewise, ‘Enabled’ will be displayed if the alarm is enabled.
High alarms will trigger when process value rises to SP or above; Low alarms will trigger when
process value falls to SP or below. The system is equipped with a short delay to avoid Low Alarms
during start-up conditions. Alarms have two categories, Warning Alarms and Shutdown Alarms.
7.8.1 Warning alarms
Flashing Button and Beeper will sound when Alarm setpoint is reached. The system will continue to
run during Alarm conditions and indicate active alarm. Alarms can be acknowledged and cleared
when Alarm Conditions are satisfied (i.e. – system operation restored within Alarm boundaries).
Shutdown alarms