Biotage Selekt Enkel User manual

Biotage®Selekt Enkel
User Manual

Biotage®Selekt Enkel User Manual | © Biotage 2022
Contents
Biotage®Selekt Enkel
User Manual
CONTENTS
System Overview
1 Columns
1 Racks and Vessels
2 Solvent Supply
3 UV Detector
3 Collection and Fractionation Methods
3 Network ShareN
4 E-mail FunctionalityN
4 Audible Alarm
4 Biotage®Safety Valve
4 Spektra Software License (Optional)
4 RFID upgrade (Optional)
4 Network Upgrade (Optional)
Prepare the System
5 Mount Column Holders
5 Inspect the Tubing
5 Start Up the System
5 Assign Solvents and Set Reservoir Volumes
6 Prime the Solvent Inlets
6 Flush the System
Set Up a Purification
7 Specify the Gradient
9 Specify the Information Parameters
9 Specify the Column Parameters
9 Specify the Collection Parameters
10 Specify the Rack Parameters
 Start, Monitor, and Control a Purification
11 Start a Purification
11 Monitor the Purification in Progress
12 Manual UV Zero
12 Start and End an Isocratic Segment
12 Add, Empty, and Replace Racks During the Run
13 Manually Fractionate
13 Line Flush, Decompression, and Detector Flush
13 Auto Extend
14 Pause, End, or Abort a Purification
14 Edit a Purification
14 Confirm Cleanup
 Unload a Purification
15 Flush the Column with Air
15 Purge the System
15 Unload the Run
15 Flush the System
15 Shut Down the System
 Results
16 Search the Results
17 Analysis of the Result
18 Customize the Report
18 Add a Report Note
19 Export the Report
19 Base a New Run on a Previous Run
 Flushes and Purge
20 Flush
20 Flow Paths
21 Air Flush
21 Purge
 Data Administration
22 Administrate Column Types
22 Administrate Solvents
23 Administrate Racks
23 Administrate Results
23 Administrate User Accounts
 System Settings
24 System
24 Purification
25 Optional Detectors
25 Network (Optional)N
25 Licenses
 Configure Network Share and E-mail Functionality
26 Configure a Network ConnectionN
26 Configure Network ShareN
28 Configure an E-mail Server ConnectionN
 Maintenance
29 Back Up and Restore the System’s Database
29 Export System Logs
30 Calibrate the UV Detector
30 Clean the Exterior of the System
30 Clean the Flow Cell of the UV Detector
31 Monthly Maintenance of the Flow Cell
31 Clean or Release Check Valves
32 Leaks
34 Replace the Fuses
34 Replace the Needle
34 Sonicate Solvent Inlet Filters
 Troubleshooting
35 Fraction Collector-Related Problems
35 Gradient Problems
35 UV Detector-Related Problems
36 Leak Detected
36 Overpressure Detected
36 Pump-Related Problems
37 QR Reader Problems
37 Contact Biotage®1-Point Support™
 Solvent Specifications
 General Information
39 Consumables and Accessories
39 Manufacturer
39 Contact Us
 Notes


System Overview
System Overview
Biotage®Selekt Enkel is an automated flash purification system
with a built-in QR reader for Biotage®Sfär columns, UV detector,
pump, fraction collector, and touch screen.
Optional hardware accessories from Biotage that can be used
with the system are:
» RFID Upgrade Kit including a reader
for Selekt collection racks,
» Extended Bed Upgrade Kit for increasing the number of
collection racks that can be placed on the system,
» Network Upgrade Kit for connecting the
system to your network and enabling network
share and/or e-mail functionality,
» Biotage®Selekt Instrument Tray with
Biotage®Solvent Detector,
» Biotage®Selekt Secondary Solvent Containment
with Biotage®Solvent Detector, and
» Biotage®Safety Valve.
There is also a Spektra software license that is sold separately
that adds features such as λ-All detection, UV baseline correction,
absorbance spectrum, and spectrum analysis (see page 4).
Columns
Columns can be placed both on the right side of the system and
on the front (up to 50g); see Figure 1.
Figure 1.Biotage®Selekt Enkel with a column on the front.
QR Reader
All Biotage®Sfär columns have a QR code that can be read
by the QR reader underneath the touch screen; see Figure 2.
When scanning a column using the QR reader, the system will
be able to trace all runs performed on the system using that
specific column based on its ID.
Figure 2.Scanning a Biotage®Sfär column.
Racks and Vessels
The system collects fractions into a variety of collection racks
and vessels. The following Selekt racks are available:
» 48 positions for 13 x 100 mm tubes
with a vessel volume of 9 mL.
» 35 positions for 16 x 100 mm tubes
with a vessel volume of 14 mL.
» 35 positions for 16 x 150 mm tubes
with a vessel volume of 22 mL.
» 28 positions for 18 x 150 mm tubes
with a vessel volume of 27 mL.
» 15 positions for 25 x 150 mm tubes
with a vessel volume of 45 mL.
» 6 positions for 120 mL flasks.
» 10 positions for 240 mL flasks.
» 8 positions for 480 mL flasks.
The predefined vessel volumes can be lowered if desired.
The collection bed can either hold a collection tray with up to
three of the racks with vessel volumes up to 120 mL (Figure 3),
or one of the 240 or 480 mL flask racks.
One collection tray is standard, but by extending the fraction
collector, it is possible to use two collection trays at the same
time. The maximum collection volume with no rack change is
3840 mL for standard bed and 7680 mL for extended bed.

Biotage®Selekt Enkel User Manual | © Biotage 2022
System Overview
RFID TagsR
All Selekt collection racks have an RFID (radio-frequency
identification) tag that can be automatically identified by the
system. The tag contains information on the rack type and the
run(s) that is/are currently in the rack, if performed on the same
system, or which system it was last used on.
Figure 3.Collection tray with three racks.
Solvent Supply
The system is equipped with two solvent inlets found on the
right side of the system (S1 and S2).
Biotage®Selekt Secondary Solvent Containment
with Biotage®Solvent Detector
An optional secondary solvent containment with a solvent
detector is available for safe unattended operation.
A maximum of four 5-L reservoirs can be placed on the optional
secondary solvent containment; see Figure 4. Larger reservoirs
than 5 L must be placed elsewhere.
Note: To be compliant with the US secondary containment
regulations, do not use reservoirs larger than 4 L.
Figure 4.The optional secondary solvent containment.
Solvent and Waste Monitoring
The system can maintain a running calculation of the fluid levels
in the reservoirs based on information entered by the user.
With solvent and waste monitoring enabled, the system will:
» Inform the user when there is not enough
solvent or waste capacity for a run.
» Issue an on-screen notification when a solvent
level is below 20% of the set capacity.
» Issue an on-screen notification when the waste
level is above 85% of the set capacity.
» Pause the system when it is time to refill a
solvent reservoir (when 10% is left) or empty
the waste reservoir (when 95% full).
Biotage®Selekt Instrument Tray with
Biotage®Solvent Detector
An optional instrument tray with a solvent detector is available
for safe unattended operation. There is one instrument tray
for systems with the standard bed (see Figure 5) and one for
systems with the extended bed.
Figure 5.The optional instrument tray with a solvent detector.
Leak Detection Using Biotage®Solvent Detector
Biotage®Solvent Detector is shipped with both the optional
secondary solvent containment (see Figure 4) and the optional
instrument tray (see Figure 5). If supported by your system*,
up to three solvent detectors can be connected to the system
using the optional solvent detector adapter cable (see Figure 6).
Figure 6
Connect to the
LEAK DET port
Connect to the
solvent detectors
1
3
2
.The optional solvent detector adapter cable (P/N 417535SP).
When a leak is detected, the user is notified (e-mail and/or
dialog), the system is paused, and all pump functionality is
disabled until the solvent detector is dry again.
* Leak detection using up to three solvent detectors is supported on all
systems that show three detectors ( ) in the About view when the
Biotage®Solvent Detector option is enabled in the system settings.
Otherwise, only one solvent detector can be used.


System Overview
The status for each solvent detector connected to the system is
displayed in the About view; see Figure 7.
Figure 7.The status of the solvent detectors are displayed in the About
view. Gray = no solvent detector connected, green = no leak detected,
and red = leak detected.
UV Detector
The system includes a 200 to 400 nm UV detector.
Collection and Fractionation Methods
Available detection signals are:
» λ-All: The system uses average absorbance
within a user-defined wavelength range for
collection and fractionation. Only available on
systems with a Spektra software license.
» UV1 and UV2: The system uses one or two wavelength
signal(s) for collection and fractionation.
Possible collection and fractionation parameters are
Collect All, Threshold, and Valley Fractionation. The system
always fractionates on volume, which is when a test tube or
bottle reaches the specified vessel volume or a user-defined
maximum fraction volume for the run. At any time, you can
manually switch to a new collection vessel by pressing in
the chromatogram.
Any signal combination can be used for collection and
fractionation. Signals that are not used for collection can be
used for monitoring.
Figure 8.The expanded Collection panel.
Network ShareN
With the system connected to the network, it is possible to give
the system access to a network share folder. The share folder
can be used for:
» Backup and restore of the system’s database.
» Export of reports and system logs; see Figure 9.
» Automatic export of the report each time a run
is completed, i.e. the reports are saved both on
the system and in the network share folder.
The system will create a subfolder in the share folder with
a name that includes the system’s serial number (Selekt_
serialnumber ). In this way the share folder can be used by
several systems.
Figure 9.The Export dialog on a system with all export options enabled.N

Biotage®Selekt Enkel User Manual | © Biotage 2022
System Overview
E-mail FunctionalityN
With the system connected to an e-mail server, it is possible for
the users to:
» Receive e-mail notifications when user interaction
is required for their runs, e.g. when a rack has to
be replaced, a solvent needs to be replenished,
a waste reservoir needs to be emptied, or a leak is
detected by the optional Biotage Solvent Detector.
» Automatically receive the report by e-mail when
their purification run has been completed.
» Send reports and logs from the system by e-mail.
Figure 10.E-mail notifications and auto send of reports are enabled and
disabled individually in each user account.N
Audible Alarm
When the audible alarm is enabled in the system settings,
a warning will sound if an error has occurred.
Biotage®Safety Valve
The optional safety valve (P/N 417115SP) enables you to use
columns or combinations (e.g. column and dry load vessel)
with a combined column volume larger than 0.8 L and up to
a maximum of 3.1 L.
The safety valve is designed to open at 8 bar and discharge
fluid until the pressure drops below 8 bar.
Spektra Software License (Optional)
The following features are included in the Spektra software
license (P/N SEL-SPK) that is sold separately:
» λ-All detection. The system uses average
absorbance within a user-defined wavelength
range for collection and fractionation.
» UV Baseline Correction. When the UV baseline
correction option is turned on, the gradient run is
preceded by a light absorbance detection phase.
During this phase, the light absorbance of the used
solvents (A and B) is measured for the whole detector
range using either the initial gradient mix to the end
mix A/B or the initial gradient mix to 100% solvent B.
The measurement results in a baseline that is subtracted
from the detector signal during the gradient run.
» Absorbance spectrum. The absorbance spectrum for
the whole detector range is displayed during the run.
» Tool for spectrum analysis. The analysis view shows the
chromatogram and the absorbance spectrum for the whole
detector range for a selected point in the chromatogram.
RFID Upgrade (Optional)
The RFID Upgrade Kit (P/N 417504SP) includes an RFID reader as
part of the system, and enables automatic recognition of Selekt
collection racks making setup easier and adding the possibility
to find results corresponding to fractions collected in a specific
rack (see “RFID TagsR” on page 2).
Functions requiring this upgrade are marked with a superscript
R in this user manual.
Network Upgrade (Optional)
With the Network Upgrade Kit (P/N 417501SP), it is possible to
enable network share and/or e-mail functionality (see “Network
ShareN” on page 3 and “E-mail FunctionalityN” above).
Functions requiring this upgrade are marked with a superscript
N in this user manual.
NRequires the Network Upgrade Kit (P/N 417501SP).
RRequires the RFID Upgrade Kit (P/N 417504SP).


Prepare the System
Mount Column Holders
The system is shipped with column holders for Sfär columns
of different sizes. Ensure that they are mounted in the desired
positions on the system.
Figure 11 shows how to mount a column holder on the right side
of the system. There are also two positions for column holders
on the front of the system that can be used for Biotage columns
up to 50g; see Figure 12.
Figure 11.Slide the column holder screw into the desired position on
the right side of the system and then place the holder over the screw
and fasten.
Figure 12.Biotage columns up to 50g can be placed on the front of
the system.
Inspect the Tubing
Warning
» Shut down the system before replacing any tubing. Use only
tubing designed for the Selekt Enkel system and supplied by
Biotage.
Inspect all tubes before each run to ensure that they do not
show signs of wear or damage and that they are properly
connected and tightened. Use caution when finger tightening
fittings to prevent stripped threads or crushed ferrules.
All external tubing on the system except for the tubing on the
collection arm can be replaced by the user.
Note: All tube types, dimensions, and lengths are essential
for the performance of the system. Only replace tubes with the
equivalent tubes designed for the Selekt Enkel system and
supplied by Biotage.
Prepare the System
Start Up the System
Turn on the system using the power switch located on the left
side of the system; see Figure 13.
Figure 13.The power switch is located on the left side of the system.
Assign Solvents and Set Reservoir Volumes
When a purification is run, the software determines which
solvent inlets are connected to the solvents used in the run.
Note: For reversed-phase purification with methanol and
water, it is strongly recommended to premix the water with
5% of methanol and degass either through vacuum or
sonication. Degassing of protic solvent blends decreases
out-gassing of entrapped air during gradient elution, which
will impact gradient performance and flow rates.
Note: With solvent and waste monitoring enabled (see
page 24), the capacity and current fluid level must be entered
each time you empty a waste reservoir or refill a solvent.
Note: Both solvent inlets must be primed with solvent to
achieve the specified pump performance.
1. In the software, press Menu and then Solvent Setup.
2. Assign a solvent to each solvent inlet using the solvent
drop-down lists (see Figure 14).
To add solvents to the list, press Solvent Administration.
For more information, see page 22.
3. If solvent monitoring is enabled, enter the capacity and
the current solvent level of each solvent reservoir.
4. If waste monitoring is enabled, enter the capacity and
the current waste level of the waste reservoir.
5. Prime the solvent inlets that have been assigned a new
solvent; see below.

Biotage®Selekt Enkel User Manual | © Biotage 2022
Prepare the System
Figure 14.The Solvent Setup window with solvent and waste monitoring
enabled.
Prime the Solvent Inlets
Before you start a purification on your system, you might need
to prime the solvent inlets to:
» Remove any air bubbles from the pump and the
solvent inlets by flushing them with solvents.
» Empty the solvent inlets of solvents used in the previous
purification and fill them with new solvents.
Note: Both solvent inlets must be primed with solvent to
achieve the specified pump performance.
Note: We recommend to have both solvent reservoirs on the
same physical height to improve accuracy in the solvent mixing.
1. In the software, press Menu and then Solvent Setup.
2. Prime the solvent inlets by pressing the corresponding
Prime buttons (see Figure 14). Note that 27 mL of the
assigned solvent is used for each prime.
Flush the System
If the automatic line flush is disabled on your system (see
page 24), ensure to flush the column flow path before each
run (see page 15).


Set Up a Purification
Warning
» Never exceed the maximum pressure or flow rate for the
used column.
» Always use Biotage Safety Valve (P/N 417115SP) when
processing columns or combinations (e.g. column and dry
load vessel) with a total CV larger than 0.8 L.
» Never use columns or combinations with a total CV larger
than 3.1 L.
» Never use the system without a column mounted or the column
inlet and outlet tubing coupled together.
1. To base the purification on a previous run, press Runs and
then see “Base a New Run on a Previous Run” on page 19.
2. Either scan your column using the QR reader underneath the
touch screen (see Figure 15) or select the column type from
the Type drop-down list in the Column panel (see Figure 16).
Note: If using a column or a combination of a column and
e.g. dry load vessel with a total CV larger than 0.8 L, a safety
valve supplied by Biotage (P/N 417115SP) must be used.
Figure 15.The QR reader is located underneath the touch screen.
3. Place the column in a column holder on the right side of the
system or on the front (up to 50g).
4. Connect the inlet and outlet tubing to the column.
5. Place the collection rack(s) that you want to use on the
collection bed. Racks with vessel volumes up to 120 mL
require the collection tray. Racks are chosen by selecting the
correct type from the Select Rack drop-down list in the Rack
panel or automatically identified by RFID when placed on the
collection bedR. (See more in “Specify the Rack Parameters”
on page 10).
6. Set up the run parameters as described in the following
sections.
Note: If the button is visible in the bottom right corner, the run
cannot be started due to e.g. that one or more run parameters
are either missing or incorrect. Press for more information.
Figure 16.The run setup view.
Specify the Gradient
The gradient can be set up using the gradient graph or the
gradient table. It is also possible to calculate a gradient from
Rfvalues obtained from TLC analysis (see page 8).
Gradient Graph and Table
Expand the gradient graph by pressing and the gradient table
by pressing .
Change the length unit by selecting CV, mm:ss, or mL from the
unit drop-down menu in the gradient table or the expanded
gradient graph.
To add a gradient segment, press the +button in the gradient
table or the expanded gradient graph. The new segment will be
added after the selected segment or node or at the end of the
gradient if nothing is selected.
To delete a node or segment, select it and press the –button in
the gradient table or the expanded gradient graph.
To increase or decrease the length of a gradient segment or
change the solvent mix, either 1) drag the segment or node to
the desired position in the gradient graph, or 2) change the
value in the gradient table.
Note that it is possible to zoom in and out of the gradient using
the pinch-to-zoom feature. To reset the zoom, press .
Set Up a Purification

Biotage®Selekt Enkel User Manual | © Biotage 2022
Set Up a Purification
Gradient Parameters
» Unit or CV/mL/mm:ss: The gradient length unit. CV (column
volumes), milliliters, or minutes and seconds; see Figure 17.
» Start: The percentage of the strong solvent at
the beginning of the gradient segment.
» End: The percentage of the strong solvent
at the end of the gradient segment.
» A-B: The solvents to be used.
» Equil./Equilibration: The column is equilibrated before the
run, unless this options is turned off. The equilibration
volume and flow rate are set automatically based
on the selected column type. To see the settings,
expand the Column panel (see Figure 21). We strongly
recommend that the equilibration is not turned off.
Figure 17.The gradient length unit can be selected in the gradient table
(as shown above) and in the expanded gradient graph (see Figure 18).
Figure 18.The expanded gradient graph. Gradient markers along the top
and right show the length and solvent percentage for the segments.
Calculate a Gradient from TLC Data
Note the following when using the TLC to Gradient editor:
» Accuracy of prediction can be reduced if using
alcohols, such as methanol and ethanol, very volatile
modifiers such as diethyl ether, or modifiers that
permanently alter the properties of the silica.
» Reversed-phase TLC is not supported.
» Slight variations in migration rates may occur if samples are
applied too close to the edge of the TLC plate. Apply samples
at least 10 mm from the edge to avoid the edge effect.
To calculate a purification gradient from the Rfvalues obtained
from TLC analysis:
1. Open the TLC to Gradient editor (see Figure 19) by
pressing TLC.
2. Select the Strong Solvent text box and enter the percentage
of the strongest (most polar) solvent in the TLC analysis.
3. Mark the solvent front of the TLC plate by sliding the Front
line to the correct position.
4. Enter the Rfvalue of the product of interest in the RfProduct
text box or by sliding the Product line to the correct position
on the TLC plate.
5. Enter the Rfvalue of the impurity closest below and above
the product in the RfImpurity 1 and RfImpurity 2 text boxes
or by sliding the corresponding lines to the correct positions
on the TLC plate. A value for RfImpurity 1 is required.
6. If you have two plates, press Add Plate and repeat steps
2 through 5 for the second plate. One plate gives a linear
gradient and two plates give a step gradient.
7. To calculate a purification gradient, press Create.
Figure 19.The TLC to Gradient editor.


Set Up a Purification
Specify the Information Parameters
» User: The name of the user. If you have not been assigned
a user name, please contact your system supervisor.
» Run Name: The name of the run. If left blank, the
name will be auto-generated based on date and
time when the purification is performed.
» Comments: Comments on the purification run. (Optional.)
Figure 20.The Information panel expanded.
Specify the Column Parameters
» Sample Mass: The crude sample mass.
» Type: The column type. Either scan the column using
the QR reader underneath the touch screen or select
a column type from the drop-down list. If a column is
scanned, the icon is shown in the Column panel.
If the column name is orange or red, its load capacity
is smaller than the entered sample mass.
Note: If using a column or a combination of a column and
e.g. dry load vessel with a total CV larger than 0.8 L, a safety
valve supplied by Biotage (P/N 417115SP) must be used.
» Flow Rate: The default (recommended) flow rate
for the selected column type is preselected.
If you want to change it, consider that the maximum
flow rate applied depends on the following:
» The maximum pressure or flow rate setting for the
column and any other accessories used in the setup.
» The maximum aspiration rate(s)
defined for the used solvent(s).
Note: If you expand the Column panel, all of the settings
for the selected column type are displayed (see Figure 21).
If the column has been scanned, the column’s ID and the
number of times it has been used is also displayed. For more
information, see “Administrate Column Types” on page 22.
Figure 21.The Column panel expanded.
Specify the Collection Parameters
» λ-All*:Select whether the average absorbance within
a user-defined wavelength range should be used for
collection and fractionation (Collect), used for monitoring
(Show), or not be shown during the run (Hide).
» UV1 and UV2: Select whether the wavelength signals should
be used for collection and fractionation (Collect), used for
monitoring (Show), or not be shown during the run (Hide).
» Wavelengths*:The shortest and longest wavelength to be
included in the λ-All signal. The range is 200 to 400 nm.
» Wavelength: The wavelength to be used for
UV1 and UV2. The range is 200 to 400 nm.
» Threshold: Used to collect samples when the
signal level exceeds the set threshold.
» Collect All: Used to collect the entire run.
» UV Baseline Correction*: When this option is turned on,
the gradient run is preceded by a light absorbance
detection phase. During this phase, the light absorbance
of the used solvents (A and B) is measured for the
whole detector range using either the initial gradient
mix to the end mix A/B or the initial gradient mix to
100% solvent B (see “System Settings” on page 24).
The measurement results in a baseline containing the
maximum absorbance of the solvents. During the gradient
run, the baseline is subtracted from the detector signal
affecting UV1, UV2, λ-All, and the absorbance spectrum.
*Only available on systems with a Spektra software license.

 Biotage®Selekt Enkel User Manual | © Biotage 2022
Set Up a Purification
Figure 22.The Collection panel expanded on a system with a Spektra
software license.
Specify the Rack Parameters
Load Racks
Rack type is selected by expanding the Rack panel
(see Figure 23) and selecting the correct rack in the
corresponding Select Rack drop-down list.
Selekt racks can be automatically identifiedRby the system
when placed on the collection bed. RFID racks have the icon
to their right in the expanded Rack panel (see Figure 31 on
page 13).
Figure 23.The Rack panel expanded on a system with extended
collection bed. Rack A was used in the previous run but still has empty
vessels that can be used by the next run. Rack C is disabled and will not
be used for collection. The Select Rack drop-down list shows the available
rack types.
Assign Racks to the Run
To assign or remove a rack from the run, expand the Rack panel
(see Figure 23) and set/clear the check box in front of the rack.
The following racks will automatically be assigned to your run:
» A rack that you select in the Rack panel.
» A rack that was assigned to but not used by the previous run.
» The last rack used in the previous run, if it still
has one or more empty vessels or rows that are
allowed to be used in the next run according to the
system settings (see “Rack Sharing”below).
Note: If you do not want to share the last rack used in the
previous run, either remove it from the system by selecting
None in the corresponding Select Rack drop-down list or clear
the check box in front of the rack.
Rack Sharing
The system offers the possibility to have the first fraction(s) of
a run collected in the last rack used by the previous run if the
following applies:
» The Start Collection parameter in the system settings
is set to First Empty Row or First Empty Vessel.
» The last rack used in the previous run:
» Contains at least one row of empty vessels
or one empty vessel, depending on the Start
Collection setting (see previous bullet).
» Has not already been shared by five runs.
» Is enabled in the Rack panel when the run is started.
Figure 24.There are two Start Collection options in the system settings
that allow two consecutive runs to share a rack, First Empty Row and First
Empty Vessel. The first collection vessel to be used is highlighted with
a thicker border.
Collection Order
The system uses the assigned racks in alphabetical order in
regard to their positions on the tray. The dispensing order is
left to right starting with the front vessel row. The first vessel
that will be used for the run is highlighted with a thicker border;
see Figure 24.
Note: If the run is sharing a rack with the previous run,
collection will start in the shared rack independent of the
positions of other racks assigned to the run. For more
information see “Rack Sharing” above.
Max Fraction Volume
If you want to use a lower fraction volume than the one defined
for the selected rack(s), expand the Rack panel, enable the
Max Fraction Volume option (see Figure 23), and enter the
desired volume. Note that this setting will only be applied on
racks with a higher vessel volume.


Start, Monitor, and Control a Purification
Start, Monitor, and Control a Purification
Warning
» Read and follow the safety precautions against static electricity
in the Biotage®Selekt Family Installation and Safety document
(P/N 417511) that is supplied with the system.
» Always ensure that the settings for the column selected in the
software are correct for the column to be used before starting
a run. Never exceed the maximum pressure or flow rate for the
used column.
» Never use the system without a column mounted or the column
inlet and outlet tubing coupled together.
» Keep hands away from the collection arm area until the arm has
returned to its home position (in the inner right corner) and the
system is paused or in standby.
» The system may be pressurized when paused.
» Monitor the waste reservoir to prevent overflow during operation.
Start a Purification
1. Ensure that a sufficient quantity of the correct solvent
is present in each solvent reservoir and that the waste
reservoir has sufficient capacity for the run. To view the
estimated solvent consumption for the run, press Solvents
and Waste.
2. Start the purification by pressing . If the button is
disabled, press in the bottom right corner for more
information.
If solvent and waste monitoring is enabled, you will be
notified if there is not enough solvent or waste capacity
for the run when you press .
3. When the Sample Load dialog opens, load your sample.
4. Start the gradient run by pressing the appropriate button in
the Sample Load dialog. We recommend that a gradient run
is not started until the UV lamp is sufficiently warmed up.
Figure 25.The default run view with the Spektra software license
installed.
Monitor the Purification in Progress
While a purification is running, a dynamic chromatogram and
the programmed gradient are displayed in the run view (see
Figure 25). If a Spektra software license has been installed on
your system, an absorbance spectrum for the whole detector
range is also displayed.
You can expand the chromatogram by pressing (full width) or
(full size), the spectrum (if available) by pressing and the
gradient table by pressing . Change the length unit by selecting
CV, mm:ss, or mL from the drop-down menu in the gradient
table (see Figure 25) or in the fully expanded chromatogram
(see Figure 26).
Figure 26.The chromatogram can be expanded to full width or full size.
Fractions
Fractions that are already collected can be located by matching
their colors and numbers in the chromatogram with the vessel
colors and numbers in the Rack panel (see Figure 25). Fractions
from the same peak have the same color. Fractions only
collected due to the Collect All option are colored gray.

 Biotage®Selekt Enkel User Manual | © Biotage 2022
Start, Monitor, and Control a Purification
Chromatogram
The signals and the thresholds are displayed in the
chromatogram using the following colors:
= Light absorption measured by the UV detector for
the whole λ-All range and the threshold in mAU.
(Requires a Spektra software license.)
= Light absorption measured by the detector
at wavelength UV1 and the threshold in mAU.
= Light absorption measured by the UV detector
at wavelength UV2 and the threshold in mAU.
The defined wavelengths and real-time measurements of
the absorption are displayed in the Collection panel.
Zoom in on the Chromatogram
It is always possible to zoom in and out of the gradient and
chromatogram using the pinch-to-zoom feature (see Figure 27).
It is possible to zoom in one direction (X or Y) or both directions
(X and Y). To reset the zoom, press .
Figure 27.Zoomed-in chromatogram.
Current Solvent Mix
The percentages of pumped solvents are displayed underneath
the chromatogram during the entire run (see Figure 25 and
Figure 26).
Status in the Top Pane
» Equilibration Flush: The system empties the
solvent inlets of solvents used in the previous
purification and fills them with new solvents.
» Equilibration: The system is running a column equilibration.
» Purge: The system releases the column pressure.
» Sample Load: Sample can be loaded onto the column.
» UV Warm-Up: The UV lamp is being warmed up.
» UV Zero: The system is setting the UV zero level using
the solvent mix used at the start of the gradient.
» Baseline Detection: The system is measuring the
light absorbance of the used solvents (A and B)
for the whole detector range. During the gradient
run, the baseline is subtracted from the signal.
(Requires a Spektra software license.)
» Baseline Flush: After baseline detection,
the system is flushed with the solvent mix
used at the start of the gradient.
» Gradient: The system is running a purification.
» Line Flush, Purge, and Detector Flush: At the end
of a run, i.e. after the gradient purification stage is
completed or ended by the user, the system performs
the flushes that are enabled in the system settings
(see page 24) and a system decompression (purge).
» Finished: The purification run was completed
or ended/aborted by the user.
» Failed: The purification run failed.
Manual UV Zero
During the gradient run, it is possible to manually set the
current UV absorbance level to zero AU by pressing in the
chromatogram. This feature can be enabled/disabled in the
system settings; see page 24.
Note: For systems with Spektra software license, the button
is only enabled when UV Baseline Correction is turned off in
the run setup.
Start and End an Isocratic Segment
At any time during the gradient run, you can start an isocratic
segment by enabling the Hold option in the chromatogram
(see Figure 27). End the segment by disabling the option.
Note: When using the default system settings, the isocratic
hold is limited to run for a maximum of 10 CV at the time
(see page 24). After 10 CV, the run is paused and you can
either resume the isocratic hold for up to another 10 CV or end it.
Add, Empty, and Replace
Racks During the Run
Note: To add/remove racks during a run, the run must
be paused.
If more fractions are to be collected than can fit in the available
rack(s), the system automatically pauses, the collection arm
returns to the home position (the inner right corner), and you
are prompted to load and/or assign more racks to the run.
To assign a rack to the run, expand the Rack panel and set the
check box in front of the rack.
Note: Rack sharing with another run is only possible at the
start of a run; see “Rack Sharing” on page 10.


Start, Monitor, and Control a Purification
Empty a Rack
For manually selected racks:
» When a rack is removed from the system, it also
has to be cleared from the software. This is done by
selecting None from the Select Rack drop-down list.
For RFID-identified racksR:
» When a rack that has been used in previous run(s) is
removed from the system and then reinserted, it is
automatically cleared and enabled for the run in progress.
» When a rack that has been used for the run in
progress is removed from the system and then
reinserted, it is not cleared automatically. To clear
the rack, press Empty in the Rack panel.
Rack B has been used by the run in
progress and Rack A was used in a
previous run or runs.
When replacing all vessels with empty
ones, press Empty.
When replacing the rack with another
type of rack, select the correct type from
the Select Rack drop-down list.
When removing the rack and leaving the
rack position empty, select None from
the Select Rack drop-down list.
Figure 28.Unload and empty a filled manual rack on a paused system.
Rack has been used by the run
in progress.
Rack has been removed.
Rack has been reinserted. If all vessels
are empty, press Empty.
Rack was used in a previous run or runs.
When removed and then reinserted, it is
automatically emptied in the software.
Figure 29.Unload and empty a filled Selekt rack on a paused system.R
Rack has been removed.
Rack reinserted before the run is
resumed. Collection will be resumed
in the vessel with thicker border. If all
vessels are empty, press Empty.
Rack reinserted after the run was
resumed. Empty vessels cannot be used.
If all vessels are empty, press Empty.
The rack can then be reused.
Figure 30.Unload and replace a Selekt rack currently in use.R
Resume the Run
Before you resume the run, ensure that the setup in the Rack
panel corresponds to what is loaded onto the collection bed.
The system uses the assigned racks in alphabetical order in
regard to their positions on the tray. Collection will be resumed
in the vessel that is highlighted with a thicker border in the Rack
panel; see Figure 31.
Figure 31.The Rack panel on a system with RFIDR. Rack A is used in
the current run but has been removed, and rack B is assigned to the run
but has not yet been used. If the run is resumed without rack A being in
position, the collection will be resumed in rack B.
Manually Fractionate
At any time during the gradient run, you can switch to a new
collection vessel by pressing in the chromatogram.
Line Flush, Decompression,
and Detector Flush
At the end of a run, i.e. after the gradient purification stage
is completed, the system performs a line flush (if enabled),
system decompression (purge), and a detector flush (if
enabled). To enable or disable flushes and specify whether
enabled flushes are collected or not, see page 24.
Auto Extend
If the collection criteria are still met (any signal is above
threshold level) when the system reaches the end of a
purification, the system extends the gradient purification
stage of the run with 25% of the total gradient length using
the final conditions of the run.

 Biotage®Selekt Enkel User Manual | © Biotage 2022
Start, Monitor, and Control a Purification
Pause, End, or Abort a Purification
Note: Pausing a purification may cause gradient inconsistency
due to heat, solvent and sample diffusion, etc.
Pause and Resume a Purification Run
1. To pause the purification in progress, press .
The collection arm returns to its home position (in the
inner right corner) and the system is paused.
2. To resume the run from the point at which it was paused,
press .
End the Equilibration Step or
End or Abort the Purification Run
1. Press . The collection arm returns to its home position
(in the inner right corner) and the system is paused.
2. Press .
3. When the System Paused dialog opens, select one of the
available options:
» Skip Equilibration: End the equilibration
step and move on to the gradient.
» Abort: End the purification run without
performing any flushes.
» Cleanup: End the purification run and perform the
flushes that are enabled in the system settings
and a system decompression (purge).
Edit a Purification
Edit on the Fly
The following run parameters can be changed without pausing
the purification run:
» The run comment in the Information panel.
» The flow rate in the Column panel.
» Gradient composition, length and number of segments.
» All collection parameters in the Collection panel
except for the UV baseline correction; see “Specify
the Collection Parameters” on page 9.
All changes except for changes to the gradient are instant.
When editing the gradient, the modified gradient is purple and
the ongoing gradient is black (see Figure 32). The modified/
purple gradient is applied when you press .
Figure 32.When editing the gradient while the system is processing,
the modified gradient is purple and the ongoing gradient is black.
The modified/purple gradient is applied when you press .
Pause to Edit a Purification in Progress
With the system paused, it is not only possible to change all the
run parameters that can be changed without pausing (see “Edit
on the Fly” above), but it is also possible to change the Max
Fraction Volume setting in the Rack panel.
1. Press . The collection arm returns to its home position
(in the inner right corner) and the system is paused.
2. Edit the run settings; see “Set Up a Purification” on
page 7. To undo all changes, press .
3. To apply the changes and resume the run, press .
Confirm Cleanup
If the Confirm Cleanup option is enabled on the system, the
Gradient Run Completed dialog appears before cleanup is
performed, giving the user the option to manually extend the
run (see Figure 33). To extend, press Extend Run, edit the run
settings, and then resume the run. By default the gradient is
extended with 25% of the total gradient length using the final
conditions of the run.
Figure 33.If the Confirm Cleanup option is enabled on the system,
the Gradient Run Completed dialog appears before cleanup is performed,
giving the user the possibility to extend the run. The dialog can be
minimized by pressing the arrows in the title bar.


Unload a Purification
Warning
» Never flush or purge the system without a column mounted
or the column inlet and outlet tubing coupled together.
Flush the Column with Air
Empty the column of remaining solvents using the air flush
feature; see page 21.
Purge the System
Any pressure remaining after a purification can be released
using the purge feature; see page 21.
Unload the Run
When the purification is finished, unload the column and
rack(s). To avoid leakage, plug the column inlet and outlet
fittings and couple the column inlet and outlet tubes together
(see Figure 34).
Remove Racks in the Software
Unless the system has RFIDR, racks that are unloaded have to
be removed manually in the software by selecting None in the
corresponding Select Rack drop-down list in the expanded
Rack panel.
Figure 34.When a run is finished, couple the column inlet and outlet
tubes together.
Unload a Purification
Flush the System
If the last purification of the day is performed with a
halogenated solvent (e.g. DCM), we recommend that you
assign methanol or a similar solvent to the inlet line used with
the halogenated solvent (see page 5) and flush with at least
30 mL (see page 20).
Shut Down the System
Warning
» Failure to perform an orderly system shutdown may result in
user data corruption and/or remaining pressure.
An orderly system shutdown helps prevent data corruption.
For critical applications, the use of a suitable Uninterruptable
Power Supply (UPS) may help avoid data loss during a power
outage.
1. When the system is not processing, press Menu,
Shut Down,and then Yes to confirm.
2. When the message saying it is safe to turn off the
system appears on the touch screen, turn off the system.
The power switch is located on the left side of the system;
see Figure 35.
3. If desired, unplug the power cord from the power outlet.
Figure 35.The power switch is located on the left side of the system.

 Biotage®Selekt Enkel User Manual | © Biotage 2022
Results
Results
The reports for the purifications that have been processed on
the system can be accessed by pressing Menu and then Results.
The full Selekt Enkel report contains the chromatogram,
the gradient (in the chromatogram and a table), TLC data (if
entered), run parameters, rack information, system information,
and the run log. It is also possible to add analysis pictures and
a report note after the run has been completed.
If desired, you can create a customized report where you can
disable some of the report content. Whether the full Selekt
Enkel report or the customized version will be displayed in
the Results view, depends on the setting for the Apply Report
Setup option.
The result can be analyzed by selecting the report and pressing
Analysis. The default view shows the chromatogram and rack(s)
and can be used for finding fractions. A second view, which
requires a Spektra software license, shows the chromatogram
and the absorbance spectrum for the whole detector range for a
selected point in the chromatogram. This optional view can be
used for spectrum analysis.
Search the Results
Search Criteria
The reports can be filtered on user name, chemistry type
(normal or reversed phase), and run date; see Figure 36.
To clear all filters, press . The reports are listed in
chronological order with favorites at the top. Add a report to
your favorites by pressing the star to the left ( = favorite).
Figure 36.It is possible to search for runs performed by a specific user
at a specific date or range of dates. The reports can also be filtered on
chemistry type, normal or reversed phase.
Results Based on Column ID
If you scan the Biotage columns using the QR reader, the system
will be able to trace all runs performed using a specific column
based on its ID.
To see all runs that have been performed using a column:
1. Scan the column.
2. Expand the Column panel and press Previous Runs.
The Results view opens showing all runs performed using
the scanned column.
Results Based on Rack ID
Reports for runs that currently have fractions collected in
racks on the collection bed can quickly be accessed by doing
the following.
1. Press in the Results view. The rack filter is opened in
the lower left corner; see Figure 37.
Note: The rack filter can also be opened by expanding the
Rack panel and pressing Runs on Tray.
2. In the rack filter, the following information and options
are available:
» Show Report(s): The rack contains fractions from a run
(or up to five runs if rack sharing is allowed) that has
been completed. Press the button to view the report(s).
If several racks were used in a run, the selected rack will
be highlighted in the displayed report (see Figure 37).
» In progress: The rack is being used by the run in
progress, i.e. the report is not yet available.
» Empty: The rack is empty, i.e. there is no report available.
» Show InfoR: The rack was last used on another
system i.e. there is no report available. Press
the button to view the system’s ID. The system
ID can be found in the About view.
When a manually selected rack is removed from the system,
it is cleared, meaning that results related to it cannot be
found. When a Selekt rackRis unloaded after a run has been
completed, it is automatically cleared in the software (in the
Rack panel) when returned to the system. However, as long
as the rack is not used for a new run (or it has been cleared by
the user pressing Empty in the Rack panel), the system keeps
the information on which run(s) the rack was last used in.
This information can be accessed by using the rack filter
as described above.


Results
Figure 37.The rack filter in the Results view. As two racks were used
in the selected run, the selected rack (in position C) is highlighted in the
report. (The example shows a system with an extended collection bed and
rack sharing enabled.)
Analysis of the Result
Analyze the result by selecting the report in the Results view
and pressing Analysis.
Find Fractions
In the default Analysis view, the chromatogram and rack(s) are
shown (see Figure 38). Use this view to find your fractions.
The chromatogram can be zoomed using the pinch-to-zoom
feature, and the gradient, signals and UV baseline correction (if
used) can be disable/enabled individually by pressing Options.
Fractions can be located by matching their colors and numbers
in the chromatogram with the vessel colors and numbers in
the rack(s). Fractions from the same peak have the same color.
Fractions only collected due to the Collect All option are gray.
For help finding your fractions:
» Drag the volume handle in the chromatogram to a fraction
of interest and the vessel that contains the fraction is
highlighted in the rack (purple border); see Figure 38.
The exact volume/time at which the volume handle is
positioned is displayed above the handle. If the gradient
is shown, the solvent mix is also displayed.
» Select a collection vessel in the rack (purple border) and
the volume handle in the chromatogram is repositioned
to the center of the corresponding fraction.
Analyze the Spectrum
If a Spektra software license is installed, it is possible to switch
from viewing the rack(s) to viewing an absorbance spectrum
by selecting Spectrum from the View drop-down list (see
Figure 39). In this view, you can:
» Drag the volume handle in the chromatogram to the
position/time for which you want see the absorbance
spectrum. The exact volume/time at which the volume
handle is positioned is displayed above the handle.
If the gradient is shown, the solvent mix is also displayed.
» Drag the λ1 and λ2 lines to the desired wavelengths
in the spectrum. The curves for the extra wavelengths
will be added to the chromatogram.
» Zoom the chromatogram using the pinch-to-zoom feature.
It is possible to zoom in one direction (X or Y) or both
directions (X and Y). To reset the zoom, press .
» Enable and disable the gradient, signals and
UV baseline correction (if used) individually
by pressing Options; see Figure 40.
Note: Toggling the UV Baseline Correction option only
affects the spectrum and the λ1 and λ2 signals.
Figure 38.The Analysis view showing the chromatogram and the rack(s).

 Biotage®Selekt Enkel User Manual | © Biotage 2022
Results
Figure 39.The Analysis view showing the chromatogram and the
absorbance spectrum. Requires a Spektra software license.
Figure 40.The Options dialog.
Add Analysis Pictures to the Report
It is possible to add one or more pictures of the chromatogram
with a changed zoom factor and/or enabled/disabled gradient,
signals and UV baseline correction to the report.
Each picture
includes the exact volume/time and solvent mix (if the gradient
is enabled) for the location of the volume handle and, if a
Spektra software license is installed, the spectrum.
To add a picture, press To Report in the racks or spectrum view
(see Figure 38 and Figure 41). To view the pictures that have
been taken, press View. To delete a picture, select it in the
Pictures in Report dialog and press Delete (Figure 41).
Figure 41.The Pictures in Report dialog.
Customize the Report
Customize a report by selecting it in the Results view and
pressing Report Setup. In the Report Setup view (see Figure 42),
the following content can be enabled or disabled in the report.
The changes are shown in real time.
» λ-All: The lambda all signal in the chromatogram.
» UV1: The UV1 signal in the chromatogram.
» UV2: The UV2 signal in the chromatogram.
» Gradient: The gradient in the chromatogram.
» Analysis: Pictures taken in the analysis view; see
“Add Analysis Pictures to the Report” above.
» Gradient Table: The gradient table.
» TLC Data: The TLC data.
» Racks: Rack illustrations with the fractions.
» Comments: The comments entered before the
run was started and during the run.
» Report Note: The report note that can been entered
in the Report Note field in the Report Setup view.
» Log: The run log.
» Unit: The length unit.
Note that the full Selekt Enkel report is not overwritten and
can always be accessed by turning off the Apply Report
Setup option.
Add a Report Note
Add a report note by selecting the report and pressing Report
Setup. In the Report Setup view, enter the run note in the Report
Note field and press Save (see Figure 42).