logos biosystems Luna-II yf User manual

User Manual
www.logosbio.com
LBSM-MD-ML-LUY-001
VL1712-01

DISCLAIMER
The contents of this document are subject to change without notice.
The LUNA-II YF™Automated Yeast Cell Counter is an electrical laboratory instrument for scientific research use only.
It is not a medical, therapeutic, or in vitro diagnostics device.
Do not disassemble the device on any occasion as this will invalidate your warranty.
TRADEMARKS
The trademarks used in this document are the property of Logos Biosystems, Inc.
© 2017 Logos Biosystems, Inc.All rights reserved.
FCC
COMPLIANCE
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used
in accordance with the instruction manual, may cause harmful interference to radio
communications. Operation of this equipment in a residential area is likely to cause harmful
interference in which case the user will be required to correct the interference at the user’s own
expense.
The WEEE (Waste Electrical and Electronic Equipment) symbol indicates that users of this
instrument have the responsibility of returning and disposing of WEEE in an environmentally
friendly manner. Follow the waste ordinances of your region for proper disposal provisions.
The CE mark indicates that this instrument conforms to all applicable European Community
provisions for which this marking is required. Users must be aware of and follow the conditions
described in this manual for operating the instrument. The protection provided by the instrument
may be impaired if the instrument is used in a manner not specified by this manual.
Protective earth (Ground)

2
Table of Contents
Safety Precautions ---------------------------------------------------------------------------------------
3
General Guidelines --------------------------------------------------------------------------------------
4
Environmental Conditions for Operation ------------------------------------------------------------
4
Chapter 1 –Introduction ------------------------------------------------------------------------------
5
1.1 Product Overview -------------------------------------------------------------------------------------------------------------
5
1.2 Key Features -------------------------------------------------------------------------------------------------------------------
5
1.3 Product Contents -------------------------------------------------------------------------------------------------------------
6
1.4 Product Specifications -------------------------------------------------------------------------------------------------------
6
1.5 Product Description ----------------------------------------------------------------------------------------------------------
7
1.5.1 Front View of the LUNA-II YF™------------------------------------------------------------------------------------
7
1.5.2 Rear View of the LUNA-II YF™------------------------------------------------------------------------------------
7
Chapter 2 –Setting Up --------------------------------------------------------------------------------
8
2.1 Installation ----------------------------------------------------------------------------------------------------------------------
8
2.2 Startup/Main Menu -----------------------------------------------------------------------------------------------------------
8
2.3 Settings --------------------------------------------------------------------------------------------------------------------------
9
2.3.1 Settings: Save Options ----------------------------------------------------------------------------------------------
10
2.3.2 Settings: Dilution Calculator Options ----------------------------------------------------------------------------
11
2.3.3 Settings: Date/Time --------------------------------------------------------------------------------------------------
11
2.3.4 Settings: Background Calibration --------------------------------------------------------------------------------
12
2.3.5 Settings: Software Update ------------------------------------------------------------------------------------------
12
Chapter 3 –Protocol Settings ----------------------------------------------------------------------
13
3.1 Protocol Parameters ---------------------------------------------------------------------------------------------------------
13
3.2 Creating and Editing Protocols --------------------------------------------------------------------------------------------
14
3.3 Protocol Selection ------------------------------------------------------------------------------------------------------------
15
Chapter 4 –Counting Yeast Cells -----------------------------------------------------------------
16
4.1 Instrument Preparation ------------------------------------------------------------------------------------------------------
16
4.2 Sample Preparation ----------------------------------------------------------------------------------------------------------
16
4.3 Slide Insertion -----------------------------------------------------------------------------------------------------------------
17
4.4 Color/Light Adjustment ------------------------------------------------------------------------------------------------------
17
4.5 Focusing ------------------------------------------------------------------------------------------------------------------------
18
4.5.1 Autofocusing ------------------------------------------------------------------------------------------------------------
18
4.5.2 Manual Focusing ------------------------------------------------------------------------------------------------------
18
4.6 Cell Counting ------------------------------------------------------------------------------------------------------------------
18
4.7 Results --------------------------------------------------------------------------------------------------------------------------
19
4.7.1 Results: Image View --------------------------------------------------------------------------------------------------
19
4.7.2 Results: Histogram and Gating ------------------------------------------------------------------------------------
20
4.7.3 Results: Dilution Calculator -----------------------------------------------------------------------------------------
21
4.7.4 Results: Saving and Printing ---------------------------------------------------------------------------------------
22
Chapter 5 –Review Previous Results ------------------------------------------------------------
24
Chapter 6 –Maintenance and Troubleshooting ----------------------------------------------
25
6.1 Turning On/Off -----------------------------------------------------------------------------------------------------------------
25
6.2 Cleaning -------------------------------------------------------------------------------------------------------------------------
25
6.3 Troubleshooting ---------------------------------------------------------------------------------------------------------------
26
Chapter 7 –Ordering Information -----------------------------------------------------------------
27
Chapter 8 –Purchaser Notification ---------------------------------------------------------------
28
8.1 Limited Use Label License: Research Use Only ----------------------------------------------------------------------
28
8.2 Instrument Warranty ---------------------------------------------------------------------------------------------------------
28

3
Safety Precautions
Read this manual carefully before you begin to use this instrument to ensure that you know how to operate it safely and
correctly. Use the instrument as specified by Logos Biosystems. Keep this manual in an easily accessible location for
future reference.
1. Install the instrument on a sturdy and level surface. Avoid vibrations from other devices.
2. Do not touch any components with wet hands.
3. Operate the instrument in the conditions described in the Environmental Conditions for Operation.
4. Use the components provided or authorized by Logos Biosystems. If the proper combination of
components are not used, product safety performance cannot be guaranteed.
5. Always use the power cord and AC adapter and provided by Logos Biosystems. If the proper power
cord and AC adapter are not used, the electrical safety of the product cannot be guaranteed.
6. Ensure that the input voltage is compatible with the instrument’s power supply voltage.
7. Ensure that the grounding terminal of the instrument and electrical outlet are properly connected. If the
instrument is not grounded, the electrical safety of the product cannot be guaranteed.
8. Turn the instrument on only after connecting the power cord and AC adapter to both the power source
and the instrument. Turn the instrument off before disconnecting the power cord and/or moving the
instrument.
9. Disconnect the power cord after operation or in the case of abnormalities.
10. Do not disassemble the instrument in any event. If the instrument is malfunctioning or broken, please
contact your local distributor or Logos Biosystems. Disassembling the instrument invalidates its
warranty.
11. When connecting the USB drive to a computer, be careful not to be infected by computer viruses.
12. When disposing of this instrument, check and observe the rules and regulations of your local
government.
13. Wear proper personal protective equipment (PPE) when handling stains and cell samples to avoid
exposure.
14. Do not reuse PhotonSlides™. Used slides must be disposed as biohazardous waste according to the
rules and regulations of your local government.
15. The LUNA-II YF™Automated Yeast Cell Counter is an electrical laboratory instrument for scientific
research use only. It is not a medical, therapeutic, or in vitro diagnostics device.

4
General Guidelines
Follow the instructions below to obtain the best results with the LUNA-II YF™Automated Yeast Cell Counter.
1. Hold PhotonSlides™by the edges to avoid touching the optical surface. Take care that the optical surfaces of the
slide do not become smudged, damaged, or contaminated.
2. When staining yeast cells with cell permeant nucleic acid dyes, perform counting after ten minutes of mixing
samples for accurate cell viability measurements. If necessary, count your sample twice (duplicate readings) and
take an average.
3. As the LUNA-II YF™is calibrated before shipping, recalibration before use is not necessary. See Section 2.3.4:
Settings: Background Calibration to see when background calibration is necessary.
Environmental Conditions for Operation
Operating Power
100 - 240 VAC, 1.2 A
Electrical Input
12 VDC, 3.3 A
Frequency
50/60 Hz
Installation Site
Indoor use only
Operating Temperature
10 - 35°C
Maximum Relative Humidity
10 - 80%
Altitude
≤ 2,000 m
Pollution Degree
2

5
Chapter 1 –Introduction
1.1 Product Overview
Brewing and winemaking use yeast to convert carbohydrates into alcohol in anaerobic conditions, a process called
fermentation. The quality and quantity of yeast cells “pitched” to start the fermentation process greatly affects the aroma,
clarity, and flavor of the final product. Too few yeast cells can result in soapy and chemical flavors due to byproducts
created by stressed and overworked yeast cells, incomplete fermentation, or unwanted bacterial infections. Although
more yeast pitched can lead to cleaner flavors, the variability in quantity of yeast added can make reproducing quality
and flavor between batches difficult. Some brewers and winemakers pitch by volume or weight, a highly variable and
inaccurate way of estimating the number of yeast added. Hemocytometers and microscopes are the main tools used to
analyze yeast cell count and viability, a time consuming process with a high level of user-user variability.
The LUNA-II YF™Automated Yeast Cell Counter is a fully automated solution for yeast cell counting and viability
analysis. An autofocusing liquid lens and a proven counting algorithm make yeast cell counting a simple, quick, and
reproducible task. Simply prepare a yeast sample solution and the LUNA-II YF™does the rest, doing away with the
subjectivity and time expenditure of manual cell counting.
The LUNA-II YF™produces:
the total number of cells per mL,
the number of live and dead cells per mL,
the viability of cells (% live cells to total cells),
cell images (optional: labeling live and dead cells as green and red circles, respectively).
The LUNA-II YF™automatically saves results as CSV files and provides the option to generate comprehensive PDF
reports with the date, time, protocol used, cell images, and relevant histograms. The LUNA-II YF™also provides the
option to review previous data that can be exported to USB as a CSV file.
Both reusable and disposable slides can be used with the LUNA-II YF™. Designed for cost-efficient an accurate cell
counting, the LUNA™Reusable Slide combines the economy of manual cell counting with the speed, accuracy, and
convenience of automated cell counting. The disposable PhotonSlides™are made with optical quality materials with low
autofluorescence to allow for optimal fluorescence detection. PhotonSlides™maintain the highest standard of cell
counting accuracy and offer the ultimate counting experience with no mess or cleanup.
1.2 Key Features
Key Features
Description
Dual fluorescence optics
The LUNA-II YF™uses two fluorescence channels to produce accurate cell viability
information. The intensity of each light source can be adjusted independently.
Compact, space-efficient design
Lightweight and compact, the LUNA-II YF™maximizes space and may be used on a
laboratory bench or in a biosafety cabinet.
Accuracy & precision
Sophisticated optical components and a proven counting algorithm provide accurate
and reproducible results.
Autofocusing
A non-mechanical liquid lens efficiently and reliably autofocuses on cells, removing
human error and enabling accurate cell counting.
Easy-to-operate user interface
Straightforward and intuitive software allows users to capture and analyze cell count
and viability data with ease.
Counting time
With manual focusing, you are 10 seconds away from your data. With autofocusing,
a mere 15 seconds.
Cell concentration range
Cell concentrations ranging from 5 x 104to 1 x 107cells/mL are easily analyzed.
Simple dilution calculations
Onboard software calculates dilutions for users.
Onboard memory
Up to 1000 count results can be saved directly to the LUNA-II YF™.
Customizable protocols
Up to 300 unique protocols can be set and used.
Data reports
Detailed PDF files complete with cellcount and viability data, images, and histograms
can be saved to an external drive.

6
1.3 Product Contents
The LUNA-II YF™product package contains the following components.
Upon receiving the product package, please inspect its contents to ensure that all parts have been included and that no
damage has occurred during shipping. The warranty does not cover damage that may occur during shipping and handling.
Any damage claims must be filed with the carrier. Contact your local distributor or Logos Biosystems if anything is missing.
1.4 Product Specifications
*Processing time may vary according to cell type and concentration.
**This is the minimum processing time for each focusing option at the specified concentration of Saccharomyces cerevisiae.
Component
Quantity
LUNA-II YF™Automated Yeast Cell Counter
1
Power Cord with AC Adapter
1
PhotonSlide™, 50 Slides
1 box
Acridine Orange/Propidium Iodide Stain
2 x 0.5 mL
Cell Dilution Buffer II
1 x 25 mL
USB Drive, 16 GB
1
LUNA-II YF™Automated Yeast Cell Counter Specifications
Instrument Type
Benchtop cell counter
Dimensions (W x D x H)
16 x 18 x 28 cm (6.3 x 7.0 x 11.0 in)
Weight
1.8 kg (3.9 lb)
Cell Concentration Range
5 x 104- 1 x 107cells/mL
Cell Viability Range
0 - 100%
Image Type
TIFF, 1.1 MP
Processing Time*
10** (manual focusing) or 15** (autofocusing) seconds at ~1 x 106cell/mL
PhotonSlide™Specifications
Material
Poly(methyl methacrylate) (PMMA)
Dimensions (W x D x H)
25 x 75 x 2.4 mm
Chamber Depth
100 µm
Chamber Volume
10 µL

7
1.5 Product Description
1.5.1 Front View of the LUNA-II YF™
The front of the LUNA-II YF™has a wide touchscreen, a power button, a slide port to insert slides and a USB port for
easy data transfer.
1.5.2 Rear View of the LUNA-II YF™
The rear of the LUNA-II YF™has two USB ports and a power inlet to connect the instrument to an electrical outlet.
Slide port
USB port
Power button
Touchscreen
Power inlet
USB ports

8
Chapter 2 –Setting up
2.1 Installation
Place the LUNA-II YF™on a clean and level surface. Connect the power cord to the AC adapter. Connect the power cord
to an electrical outlet after checking the outlet configuration in your local area.
Do not install the instrument in a location that will expose the instrument to intense ultraviolet light.
2.2 Startup/Main Menu
Push the power button below the touchscreen to turn the instrument on. The company logo will appear, followed by the
startup screen.
The main menu has a power icon and four options: COUNT, REVIEW, PROTOCOL, and SETTINGS.
For instructions on when and how to turn the instrument on or off, see Section 6.1: Turning On/Off.

9
2.3 Settings
The instrument is preset at the time of manufacture and may be used immediately. Users may adjust the settings of the
instrument as desired.
Select SETTINGS from the main menu.
The Settings screen displays:
a home icon: press this icon to return to the main menu,
the current protocol and date,
the date and values of the most recent calibration, and
the date and version of the latest software update.
Settings options:
2.3.1 [Save Options] Preset the types of files saved and customize the file name prefix.
2.3.2 [Dilution Calculator Options] Select the concentration value to use for dilution calculations.
2.3.3 [Date/Time] Adjust the date and time of your instrument for record keeping purposes.
2.3.4 [Background Calibration] Perform background calibrations with each software update.
2.3.5 [Software Update] Update software to the most recent version.

10
2.3.1 Settings: Save Options
Save Options allows the user to set the type of file(s) saved and the default file name prefix used for subsequent counts.
Press Save Options in the Settings screen.
There are three saving options.
Default Save
Description
Analyzed Image
Tagged image of live and dead cells
Raw Image
Untagged images of cells in each channel
Report
PDF report with count data and histograms
Select the desired saving options by pressing the boxes in front of each option. Selected boxes are yellow. Unselected
boxes are black.
Using the onscreen keyboard, enter the desired default file name prefix.
Users may add the date to the name by pressing the Add Date/Time button.
Press Save to set the default save options.
Press <to return to the Settings screen.

11
2.3.2 Settings: Dilution Calculator Options
Users may use the onboard dilution calculator to compute dilutions for subsequent experiments. When calculating
dilutions, the calculations can be made with total cell concentration, live cell concentration, dead cell concentration, or a
custom cell concentration. The Dilution Calculator Option allows the user to select which concentration value (total, live,
or dead) to use for subsequent dilution calculations.
Press Dilution Calculator Options in the Settings screen.
Select the desired value. The selection will be marked with a yellow circle.
Press <to return to the Settings screen.
2.3.3 Settings: Date/Time
The LUNA-II YF™uses a 24-hour clock and is preset to Korean Standard Time. Adjust the settings to the local date and
time for accurate record keeping.
Press Date/Time in the Settings screen.
Select the desired field to delete the existing value.
Input the desired values with the number panel on the right. Press Apply to save changes.
Press <to return to the Settings screen.

12
2.3.4 Settings: Background Calibration
Background calibration adjusts for the specific shade of the stain used for counting and is a prerequisite for the
successful detection of cells. As the LUNA-II YF™is calibrated before shipping, recalibration before use is unnecessary.
Users must recalibrate the background after each software update.
Press Background Calibration in the Settings screen.
Put 10 µL LUNA™Fluorescence Calibration Beads into the chamber of a new PhotonSlide™or a LUNA™Reusable Slide.
Wait for 30 seconds to 1 minute for the beads to settle down.
Insert the slide face up and sample-side first into the slide port.
Important! Do not insert the slide facedown.
Press OK. Do not remove the slide or turn off the instrument during this process
The background calibration value and date will have changed in the Settings Screen.
2.3.5 Settings: Software Update
Logos Biosystems continually provides software updates to ensure optimal performance. The existing version of
software is displayed in the startup screen and the Settings screen.
Download the most recent version from the Logos Biosystems website (www.logosbio.com) into the root directory of a
compatible USB drive.
Press Software Update in the Settings screen.
Insert the USB drive with the downloaded file into the USB port.
Press Start. Do not turn the instrument off during the update.
The date and version of the last software update will change automatically in the Settings screen.
Important! Users must recalibrate the background after each software update (see Section 2.3.4: Settings:
Background Calibration).

13
Chapter 3 –Protocol Settings
The LUNA-II YF™provides two default protocols that can be used for most common yeast cells. Users may create and
save up to 300 unique protocols.
3.1 Protocol Parameters
LUNA-II YF™protocols have the following parameters:
Parameter
Range
AO-PI
FDA-PI
Dilution Factor
1-10
1.11
1.18
Min. Fluorescent Object Size (µm)
1-59
1
1
Max. Fluorescent Object Size (µm)
2-60
60
60
Green Fluorescence Exposure
1-10
7
4
Red Fluorescence Exposure
1-10
3
6
Dilution Factor: The dilution factor must be set properly prior to counting to ensure accuracy.
Final sample volume*
Dilution factor = ------------------------------------------ .
Yeast suspension volume
*final sample volume = yeast suspension volume + reagents volume
The default dilution factor is preset as 1.11 for the AO-PI protocol and 1.18 for the FDA-PI protocol, assuming a 9:1 and
17:3 ratio of yeast suspension to reagents, respectively (see Section 4.2 Sample Preparation). Assuming a final sample
volume of 20 µL, users can modify this value according to the following table:
Yeast Volume
20 µL
18 µL
17 µL
16 µL
15 µL
14 µL
12 µL
10 µL
8 µL
6 µL
4 µL
2 µL
Dilution Factor
1
1.11
1.18
1.25
1.33
1.42
1.66
2
2.5
3.33
5
10
For users handling highly dense cultures, serial dilutions and several counts with appropriately adjusted dilution factors
will be necessary.
Minimum and Maximum Fluorescent Object Size: Fluorescent object size measures the diameter of the fluorescent
signal from the nucleic acid stains. This does not correspond to the physical size of the cell. Users can adjust the
parameters in 1 µm increments between 1-60 µm.
Green and Red Fluorescence Exposure: The exposure for each channel can be adjusted. Increase exposure if the
preview image is dim and only a few cells are visible. Lower exposure if the preview image is too bright and background
noise is high. Determine optimal exposure values empirically. Higher exposure levels lead to slower frame rates in the
live view of cells.

14
3.2 Creating and Editing Protocols
Select PROTOCOL from the main menu.
The Protocol screen includes a list of saved protocols. The selected protocol is highlighted in blue. The parameters of
the selected protocol are displayed in the right panel.
The AO-PI and FDA-PI protocols cannot be modified or deleted.
To create a new protocol, select one of the default protocols and press Save as. Using the onscreen keyboard, rename
the protocol and press Save.
The newly created protocol will appear in the list of protocols in the Protocol screen.
To modify the new protocol, press Edit.This will activate the arrows for each parameter, turning them black. Press the
arrows to adjust the values of each parameter as desired.
Press Save as.
Press Delete to delete a selected protocol.

15
3.3 Protocol Selection
When staining cells with Acridine Orange and Propidium Iodide, select theAO-PI protocol. When staining cells with
Fluorescein Diacetate and Propidium Iodide, select the FDA-PI protocol.
Select the desired protocol in the Protocol screen.
Press Load to apply the selected protocol.
Now the instrument is ready to count cells with the selected protocol.
Important! Merely selecting a protocol does not mean that it has been put into effect. To apply the selected
protocol, make sure to press Load.

16
Chapter 4 –Counting Yeast Cells
4.1 Instrument Preparation
Select COUNT from the main menu.
The set protocol, date, and time appear in the panel at the top of the Count screen.
Set the desired protocol prior to counting (see Section 3.3: Protocol Selection).
4.2 Sample Preparation
Prepare a yeast suspension according to standard procedures. Mix gently but thoroughly to ensure that the suspension
is homogenous. For yeast samples that are highly dense and/or were cultured in YPD broth, dilute the sample by at least
1:100 with Cell Dilution Buffer II prior to counting. YPD has a component that has strong esterase activity, which inhibits
dye uptake into cells, leading to a weak fluorescence signal. Cell Dilution Buffer II increases cell membrane permeability,
leading to increased levels of dye uptake.
[AO-PI] Mix 18 µL yeast suspension with 2 µLAcridine Orange/Propidium Iodide Stain. Pipette gently. Incubate
the sample for 10 minutes at room temperature.
[FDA-PI] Mix 17 µL yeast suspension with 1 µL Fluorescence Signal Enhancer 1*, 1 µL Fluorescein Diacetate
Stain and 1 µL Propidium Iodide Stain for Yeast. Pipette gently. Incubate the sample for 10 minutes at room
temperature.
*Fluorescence Signal Enhancer 1 is an inhibitor of ATP-dependent fluorescein efflux. When used in proper conditions, the enhancers
will lower background and increase signal intensity, thereby enhancing the overall signal-to-noise ratio.
Prepare a new PhotonSlide™or a clean LUNA™Reusable Slide. Hold the slide by its edges and load 10-12 µL of the cell
sample into a sample chamber. For easy and accurate loading, hold the pipette at a 45-60°angle to the slide. Be careful
not to over-load or under-load the sample chamber.
Depending on sample conditions, wait for 30 seconds to 1 minute for the cells to settle within the chamber.
Important! Prolonged incubation in Cell Dilution Buffer ll can affect cell viability. Do not expose the cells to the
buffer for more than 30 minutes prior to counting.

17
4.3 Slide Insertion
Insert the slide face up and sample-side first into the slide port of LUNA-II YF™. The LUNA-II YF™can only analyze the
inserted chamber.
Important! Do not insert the slide facedown.
4.4 Color/Light Adjustment
The LUNA-II YF™starts with the light turned off to prevent photobleaching.
Press [Light] to turn on the light.
A magnifier button, fluorescence channel button, and light control bar will appear to the right of the screen.
The GF button indicates that the green fluorescence channel is on.
Press GF to change it to RF and switch to the red fluorescence channel.
To view the stained cells, users may adjust the intensity of each channel as needed by pressing the light control bar
arrow heads (up or down).
Important! Adjusting the intensity levels may affect counting results. Users should only adjust intensity levels to
visualize cells prior to counting. For optimal counting, maintain intensity level 7. If cell images captured during
counting are too dim, adjust exposure levels in the Protocol screen.

18
4.5 Focusing
The LUNA-II YF™has an autofocusing algorithm that works in tandem with a focusing mechanism to rapidly obtain the Z
position of the sample by applying a small voltage to a liquid lens. The elimination of mechanical parts in the focusing
mechanism removes noise and significantly reduces the need for servicing.
4.5.1 Autofocusing
Press the circle next to [Autofocused Counting]. The circle will turn yellow when the autofocus is activated.
4.5.2 Manual Focusing
Users can adjust the focus manually by simply pressing the [Focus] arrow heads (up or down) with the autofocus
function on or off.
Zoom in to the image using the magnifier button to achieve the best focus.
4.6 Cell Counting
Use a finger or a stylus to navigate the image. The red outer box in the top left corner of the image represents the entire
counting area and the inner box is the current field of view. The size and location of the inner box will change with the
magnification and movement of the screen
Press the magnifier button to zoom in and out of the image.
Make sure the cells are not moving. If some cells are still moving, wait for all cells to settle. While waiting, turn off the
light to prevent photobleaching.
Press [Count] to start counting. The light will turn on automatically.
The LUNA-II YF™counts the cells in 0.7 µL, which is comparable to seven squares on a standard hemocytometer.
Counting time will vary depending on the protocol and cell concentration. With the default protocols, cell samples with a
concentration of ~1 x 106cell/mL will take at minimum 10 seconds to count without autofocusing or 15 seconds with
autofocusing.
Cell count and viability results will appear.

19
4.7 Results
The LUNA-II YF™has onboard data analysis software that allows users to analyze data immediately.
4.7.1 Results: Image View
*Average size indicates the fluorescent object size.
Press Image to view the captured image of the analyzed cell sample.
Use a finger or a stylus to navigate the image.
The Tag, magnifier, and image selection buttons are to the right of the image.
Users can view the raw images captured in each channel and a merged image of both channels. Press Overlay to
change it to GF and display live cells. Press GF to change it to RF and display dead cells. Press RF to change it to
Overlay and display a merged image of both live and dead cells.
Press Tag in the merged image to label what was counted as live cells with green circles and dead cells with red circles.
The Tag function allows users to verify the instrument’s counting accuracy immediately. Press Tag again to remove the
labels. The Tag function works for the merged image but not In the GF and RF images.
Press the magnifier button to zoom in and out of the saved image.