Malvern Nano Series User guide

Printed in England MRK1378-01
Malvern Instruments Limited
Enigma Business Park
Grovewood Road, Malvern
Worcs, WR14 1XZ, U.K.
Tel: [phone]
Fax: [phone]
www.malvern.com
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Zetasizer
Malvern
nano series
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Zetasizer Nano
Basic Guide
MAN0486 Issue 1.0 September 2012
English
Zetaszier Nano Basic Guide (Man0486-1.0).book Page i Monday, September 10, 2012 2:19 PM

Copyright © 2007, 2009, 2012 Malvern Instruments Ltd.
Malvern Instruments pursues a policy of continual improvement due to technical development. We
therefore reserve the right to deviate from information, descriptions, and specifications in this
publication without notice. Malvern Instruments shall not be liable for errors contained herein or
for incidental or consequential damages in connection with the furnishing, performance or use of
this material. No reproduction or transmission of any part of this publication is allowed without the
express written permission of Malvern Instruments Ltd.
Head office:
Malvern Instruments Ltd.
Enigma Business Park,
Grovewood Road,
Malvern,
Worcestershire WR14 1XZ
United Kingdom.
Tel + [44] (0)1684-892456
Fax + [44] (0)1684-892789
Zetasizer, Malvern and the 'hills' logo are registered trademarks in the UK and/or other countries,
and are owned by Malvern Instruments Ltd.
NIBS and M3-PALS are trademarks of Malvern Instruments.
M3 is granted Euro Pat No: 1 154 266 DE FR.
Windows is a registered trademark of Microsoft Corporation.
Tygon is a registered trademark of Saint-Gobain Corporation.
Hellmanex is a registered trademark of Hellma GmbH & Co. KG.
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Zetasizer Nano Basic Guide Page i
Introduction to this manual
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Access to the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Assumed information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Where to get help . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
Site requirements
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Environmental conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
Space required. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
Services. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
MPT-2 Titrator services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Computer specification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
Health and safety
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
General safety issues . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
Power cords and Power safety . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
Moving the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
Maintenance of the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
Sample handling warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
Disposal of the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
System Overview
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
Briefly... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
The Zetasizer Nano hardware . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
The Zetasizer software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
Connecting the Zetasizer Nano . . . . . . . . . . . . . . . . . . . . . . . . . . 4-12
Power on . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-13
Table of contents
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Table of Contents Zetasizer Nano Basic Guide
Page ii MAN 0486
Performing a quick measurement. . . . . . . . . . . . . . . . . . . . . . . . . 4-14
Next... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-25
Appendices
Specifications - Optical unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1
Chemical compatibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-4
Regulatory Statements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-5
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Zetasizer Nano Basic Guide Page 1-1
1
Introduction
to this manual
Introduction
This manual covers the operation and maintenance of the Zetasizer Nano particle
analyser series.
In addition, dependent upon the instrument model, the Zetasizer Nano can meas-
ure other key parameters such as Microrheology, Titration and Protein mobility.
Instruments with a black and red badge fitted to the instrument cover either
have a 4mW 632.8nm ‘red’ laser or a 10mW 632.8nm ‘red’ laser (Nano ZSP
only) fitted.
Instruments with a green badge have a 532nm 'green' laser.
High Temperature instruments have ‘HT’ on the main instrument label. High
Temperature and other build options exist for all the above instruments.
Zetasizer Nano
instrument
Model
number Measurement types
Nano S (Red badge) ZEN1600 Particle Size and Molecular weight
Nano S (Green badge) ZEN1500 Particle Size and Molecular weight
Nano Z (Red badge) ZEN2600 Zeta potential
Nano Z (Green badge) ZEN2500 Zeta potential
Nano ZS (Green badge) ZEN3500 Particle Size, Molecular weight and Zeta
potential
Nano S90 (Red badge) ZEN1690 Particle Size - 90° optics
Nano ZS90 (Red badge) ZEN3690 Particle Size and Zeta potential - 90°
optics
Nano ZSP (Red badge) ZEN5600 Particle Size, Molecular weight and Zeta
potential
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Chapter 1 Introduction to this manual
Page 1-2 MAN 0486
1
Note
For the Zetasizer model, serial number, software and firmware version,
left-click the Nano icon in the right corner of the status bar.
Using this manual
Warning!
Read the Health and Safety chapter before operating the instrument.
Read this manual in conjunction with the main Zetasizer Nano User manual,
the Zetasizer Nano Accessories guide and the appropriate dispersion unit man-
uals.
For more detail on the software, use its online Help. Each window has a Help but-
ton giving further information on how to use its features.
If using the MPT-2 Titrator and Degasser, refer to the Titrator and Degasser
user manual where necessary.
Access to the instrument
Malvern personnel
Malvern personnel (service engineers, representatives, etc.) have full access to the
instrument and are the only people authorised to perform all service procedures
that may require the removal of the covers.
Warning!
Removal of the main covers by unauthorised personnel, even a supervisor,
will invalidate the warranty of the instrument.
Supervisor
The supervisor is responsible for the management and safety of the instrument and
its operation. The supervisor also trains the operators and can perform user mainte-
nance routines. The supervisor has access to a more detailed manual in English.
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Introduction to this manual Chapter 1
Zetasizer Nano Basic Guide Page 1-3
Operator
An operator is a person trained in the use of the system. The operator can perform
some user maintenance routines identified.
Warning!
Failure to follow these guidelines could result in exposure to hazardous
voltages and laser radiation.
Assumed information
General
The Zetasizer Nano can be used with a variety of Zetasizer Nano accessories or
dispersion units that allow it to measure a variety of samples. The basic function of
these is to prepare and deliver the sample to the optical unit for measurement.
For more details of any sample dispersion unit, refer to its manual.
Naming convention
Within this manual:
The Zetasizer Nano is referred to as “the optical unit” or “the instrument”.
The accessories or sample dispersion units are referred to as “MPT-2 Titrator”,
“the vacuum degasser”, or as “the accessory” or “the dispersion unit”.
The combination of the optical unit, one or more accessories or dispersion
units and the computer is referred to as “the system”.
Menu commands
Software menu commands are referred to in the form main menu-menu item.
As an example, the command Configure-New SOP refers to selecting the New
SOP item in the Configure menu. Menu commands are shown in bold text.
Where to get help
This section describes the available sources of information on the system. The pri-
mary sources of information on the system are instrument and accessory manuals
and the software’s help system. The manuals give an overview of the system as a
whole, while the online help system gives more detailed information on the Zeta-
sizer software. Some features are described in both the manuals and Help system.
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Chapter 1 Introduction to this manual
Page 1-4 MAN 0486
Manuals
The following manuals are available for the Zetasizer Nano:
Basic Guide
A concise yet essential guide that provides an introduction to the system and its
usage covering Site requirements, Health and Safety and an instrument and
accessory overview. Finally a brief guide to making a measurement and view-
ing the results is included.
User Guide
The primary source of information about the Zetasizer Nano hardware and its use.
See About this manual earlier in the chapter.
Accessory and dispersion unit guides
Separate guides are supplied with the various Nano accessories and Titrator disper-
sion units. These provide essential information on the connection, operation and
maintenance of the dispersion units.
Help System
Each key software window has a Help button giving information specific to it.
Additional Info Tips are also available whenever is shown - simply move the
mouse pointer over this icon to see a relevant tip.
Technical support
Malvern Instruments offers several methods of technical support, which are
described in this section.
Email support
Note
This is the primary support method – only use the other methods if this is
not available at your location.
When contacting your local Malvern Instruments representative - details of all local
telephone numbers are available at www.malvern.com. Always quote the following
information:
Model and serial number of the instrument. The serial number is shown
on the Zetasizer software status bar - rollover the Nano icon to display.
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Introduction to this manual Chapter 1
Zetasizer Nano Basic Guide Page 1-5
The software version. To find this select Help-About in the software -
alternatively, move the mouse over the instrument status bar icon. The Appli-
cation Version is the main firmware version number to note.
Additional support
Contact the International Malvern Helpdesk if the local Malvern Instruments
representative is not available:
Telephone: [phone] or email: [email protected].
Note
This help line is primarily English speaking.
Remote support
Malvern Instruments offers a remote support service, delivered by an internet con-
nection. Benefits include fast and efficient fault diagnosis, as well as reducing
downtime and costs.
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Chapter 1 Introduction to this manual
Page 1-6 MAN 0486
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Zetasizer Nano Basic Guide Page 2-1
2
Site requirements
Introduction
This document outlines the site requirements for a Zetasizer Nano. Ensure all
these are met before the Malvern Instruments engineer arrives to install and
commission the system.
Environmental conditions
The site must be:
Away from strong light sources (e.g. windows).
Away from strong heat sources (e.g. radiators).
Well ventilated (for noxious samples).
On a horizontal vibration-free bench built to support the weight of the system
(shown below):
Unit Weight
Zetasizer Nano 21kg
MPT-2 Titrator 5.3kg
Vacuum degasser 2.75kg
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Chapter 2 Site requirements
Page 2-2 MAN 0486
2
Store/operate the system in the following conditions (accurate measurements are
sample-dependent, for example dry powders may stick together in high humidity):
In addition:
Do not obstruct power sockets as they may need to be disconnected during an
emergency.
Avoid passing electrical cables through areas where liquids can be spilt.
Space required
Provide enough space to allow easy access to all components and connections.
Allow at least 800mm above the bench surface for access to the cell area and
accessories.
Component dimensions are: (the width is with the cuvette holder closed):
This diagram shows the minimum recommended space required for a typical
system using an MPT-2 Titrator, and its computer.
IP rating Designed to meet IP41B
Operational conditions 5°C to 40°C (41°F to 104°F)
Storage conditions -20°C to 50°C (-4°F to 122°F)
Humidity Maximum humidity 80% for temperatures up to 31°C,
decreasing linearly to 50% relative humidity at 40°C
Usage Indoor use only
Altitude Up to 2000m
Mains supply voltage
fluctuations
Up to ±10% of nominal voltage
Overvoltage category II (IEC 60664)
Pollution degree 2 (BS EN 60664-1:2003)
Installation category II (BS EN 60664-1:2003)
Component Width Depth Height
Zetasizer Nano 320mm 622mm 260mm
MPT-2 Titrator 170mm 390mm 260mm
Vacuum degasser 75mm 250mm 130mm
Computer and printer S e e m a n u f a c t u r e r ’ s d o c u m e n t a t i o n
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Site requirements Chapter 2
Zetasizer Nano Basic Guide Page 2-3
ill 7931
Services
This section describes the services required.
General
The following services are required for each component:
In addition, the computer system requires one or more power sockets.
Power specification
The mains power supply must be clean and filtered. If necessary, fit an Un-
interruptible Power Supply (UPS) to remove any spikes or noise.
The power requirements are:
Component Zetasizer Nano MPT-2 Titrator
Vacuum
degasser
Power sockets 111
Unit Power requirement
Zetasizer Nano ~ 100-240V, 50-60Hz
MPT-2 Titrator ~ 100-240V, 50-60Hz
Vacuum degasser ~ 100-240V, 50-60Hz
300 mm
150 mm
150 mm 300 mm
1240 mm
750 mm
800 mm
(maximum)
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Chapter 2 Site requirements
Page 2-4 MAN 0486
Note
Only use the PSU/cables provided. Using another PSU voids any war-
ranty and may be unsafe.
Additional services
Laser safety
Zetasizer Nano instruments are Class 1 laser products and as such, require no spe-
cial laser safety considerations during normal operation. However, during servicing
(which must be performed by a qualified Malvern representative), the servicing
engineer may be exposed to class 3b, or above, laser radiation. We therefore recom-
mend that the administrative controls recommendations of the Laser Safety Regu-
lations (IEC 60825-1(1993) +A1(1997)+A2(2001) are implemented.
Purge specification
If measuring samples at low temperatures there is a risk of condensation occurring
on the cell; this occurs when the measurement temperature is less than the ‘dew
point’ of the ambient air surrounding the cell being measured. This is particularly
likely in humid climates.
The purge inlet port can be used to connect a dry air supply to the instrument, i.e. a
supply with a dew point below the target temperature. This removes any moisture
in the air immediately surrounding the cell and prevents condensation. The air
supply must conform to the following specification:
Compressed air to DIN 8573-1
Oil = Class 1
Water = Class 3
Particulate = Class 3
Pressure = 100 kPa g
For connection purposes, the purge connection uses an M5 internal thread.
Caution!
The purge air line supply must conform to the above specification. Failure
to meet this specification may result in permanent damage to the instru-
ment and invalidate the warranty.
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Site requirements Chapter 2
Zetasizer Nano Basic Guide Page 2-5
MPT-2 Titrator services
Nitrogen purge specification
Warning!
A Nitrogen supply must be used in a well ventilated environment.
The MPT-2 Titrator has a purge connector for connection of a Nitrogen purge
supply. This can be used to blanket the area directly above the sample and prevent
any absorption of Oxygen that may change the pH characteristics of the sample, i.e.
cause a pH drift.
If a Nitrogen supply is required it must conform to these specifications:
The Nitrogen supply must be dry, free from oil and filtered to remove any
contaminants that could affect the sample.
The flow rate should be adjustable between 2 and 20 ml/min.
Computer specification
Contact the Malvern Helpdesk or website for the recommended computer
specification. This is also provided in the Software Update Notification.
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Chapter 2 Site requirements
Page 2-6 MAN 0486
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Zetasizer Nano Basic Guide Page 3-1
3
Health and safety
Introduction
This chapter provides Health and Safety information. All users must read this
section. The topics covered are:
General safety issues
Laser safety issues
Power cords and power safety
Moving the system
Maintenance of the instrument
Sample handling warnings
Disposal of the instrument
General safety issues
Warning!
Use of the system in a manner not specified by Malvern Instruments may
impair the protection provided by the system.
Site requirements
The system has specific site requirements that must be enforced to ensure safe
operation of the instrument - refer to the Site requirements chapter.
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Chapter 3 Health and safety
Page 3-2 MAN 0486
3
Warning!
Safety may be compromised if the system is used in an environment not
conforming to the site requirements.
Positioning the Instrument
Warning!
Do not position the instrument such that the power cord, where it exits the
product, is unreachable for disconnection.
Warning!
Do not obstruct the ventilation slots underneath the instrument, nor the
fans on the rear panel. Restricting airflow can damage the instrument or
cause overheating.
Purge warnings (MPT-2 Titrator)
Warning!
If a Nitrogen supply is used the system must be located in a well ventilated
environment. Turn off the supply when not in use.
Temperature warnings
Warning!
The warning triangles on the cuvette lid and thermal cap warn of poten-
tially hazardous temperatures within the cell area. The temperature range
of the cell area is 2°C to 90°C for the standard instrument, 2°C to 120°C
for the High Temperature option.
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