Malvern MicroCal iTC200 User manual

MicroCal iTC200 system
Operating Instructions
Original instructions

.
Copyright © 2014 Malvern Instruments Ltd.
MAN0558-01-EN-00 August 2014
Malvern Instruments pursues a policy of continual improvement due to technical
development. We therefore reserve the right to deviate from information, descriptions,
and specifications in this publication without notice. Malvern Instruments shall not be
liable for errors contained herein or for incidental or consequential damages in
connection with the furnishing, performance or use of this material. No reproduction
or transmission of any part of this publication is allowed without the express written
permission of Malvern Instruments Ltd.
Head office:
Malvern Instruments Ltd.
Grovewood Road,
Malvern,
Worcestershire, WR14 1XZ
United Kingdom.
MicroCal, Malvern and the 'hills' logo are registered trademarks in the UK and/or other
countries, and are owned by Malvern Instruments Ltd
Windows is a registered trademark of Microsoft Corporation
Tel
Fax
[phone]
[phone]

Table of Contents
51 Introduction ..........................................................................................................
61.1 About this manual ................................................................................................................................
71.2 Important user information .............................................................................................................
101.3 Regulatory information ......................................................................................................................
132 Safety instructions ...............................................................................................
142.1 Safety precautions ...............................................................................................................................
222.2 Labels .........................................................................................................................................................
262.3 Emergency procedures ......................................................................................................................
272.4 Recycling information .........................................................................................................................
282.5 Declaration of Hazardous Substances (DoHS) ........................................................................
313 System description ..............................................................................................
323.1 MicroCal iTC200 system .......................................................................................................................
333.2 Illustrations ..............................................................................................................................................
364 Installation ............................................................................................................
384.1 Site requirements ..................................................................................................................................
414.2 Transport ..................................................................................................................................................
424.3 Hardware connections .......................................................................................................................
445 Operation ..............................................................................................................
475.1 Procedure before an experiment ..................................................................................................
495.2 Creating a method ...............................................................................................................................
525.3 Loading the titration syringe ...........................................................................................................
535.4 Loading the cell .....................................................................................................................................
545.5 Performing an experiment ...............................................................................................................
565.6 Procedures after an experiment ....................................................................................................
586 Maintenance .........................................................................................................
616.1 Cleaning the cell ....................................................................................................................................
626.2 Refilling the reference cell ................................................................................................................
636.3 Replacing the syringe plunger tip .................................................................................................
696.4 Replacing and cleaning the titration syringe ...........................................................................
767 Troubleshooting ...................................................................................................
777.1 How to get help .....................................................................................................................................
797.2 Troubleshooting chart ........................................................................................................................
808 Reference information ........................................................................................
83Index .......................................................................................................................
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MicroCal iTC200 System Operating Instructions MAN0558

1 Introduction
About this chapter
This chapter contains important user information, descriptions of safety notices, regula-
tory information, intended use of MicroCal iTC200, and lists of associated documentation.
In this chapter
This chapter contains the following sections:
See pageSection
61.1 About this manual
71.2 Important user information
101.3 Regulatory information
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1 Introduction
MicroCal iTC200 System Operating Instructions MAN0558

1.1 About this manual
Purpose of the Operating
Instructions
The Operating Instructions provide the user with the instructions needed to install, operate
and maintain MicroCal iTC200 in a safe way.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (e.g., Power switch).
6 MicroCal iTC200 System Operating Instructions 28-9639-77 AD
1 Introduction
1.1 About this manual
MicroCal iTC200 System Operating Instructions MAN0558

1.2 Important user information
Read this before operating
MicroCal iTC200
All users must read the entire Operating Instructions before installing, operating or
maintaining the system.
Always keep the Operating Instructions at hand when operating MicroCal iTC200.
Do not operate MicroCal iTC200 in any other way than described in the user documenta-
tion. If you do, you may be exposed to hazards that can lead to personal injury, you may
cause damage to the equipment, and no warranty will apply.
Intended use of MicroCal iTC200
MicroCal iTC200 is an Isothermal Titration Calorimeter system designed for bio-molecular
interaction studies in research applications.
MicroCal iTC200 is intended for research use only and shall not be used in any clinical
procedures or for diagnostic purposes.
MicroCal iTC200 is not suitable for operation in a potentially explosive atmosphere or for
handling flammable liquids.
WARNING
Do not operate MicroCal iTC200 in any other way than described in
MicroCal iTC200 user documentation.
Prerequisites
In order to operate MicroCal iTC200 safely and according to the intended purpose the
following prerequisites must be met:
•You should have a general understanding of the use of a personal computer running
Microsoft™ Windows™ in the version provided with your product.
MicroCal iTC200 System Operating Instructions 28-9639-77 AD 7
1 Introduction
1.2 Important user information
MicroCal iTC200 System Operating Instructions MAN0558

•You should be acquainted with the use of general laboratory equipment and with
handling of biological materials.
•You must read and understand the Safety instructions chapter of these Operating
Instructions.
•The system must be installed according to the instructions in Chapter 4 Installation,
on page 36.
Safety notices
This user documentation contains WARNINGS, CAUTIONS and NOTICES concerning the
safe use of the product. See definitions below.
Warnings
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
Cautions
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to
proceed until all stated conditions are met and clearly understood.
Notices
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Note:
8 MicroCal iTC200 System Operating Instructions 28-9639-77 AD
1 Introduction
1.2 Important user information
MicroCal iTC200 System Operating Instructions MAN0558

A tip contains useful information that can improve or optimize your procedures.
Tip:
MicroCal iTC200 System Operating Instructions 28-9639-77 AD 9
1 Introduction
1.2 Important user information
MicroCal iTC200 System Operating Instructions MAN0558

1.3 Regulatory information
Introduction
This section lists the directives and standards that are fulfilled by MicroCal iTC200.
Manufacturing information
The table below summarizes the required manufacturing information. For further infor-
mation, see the EC Declaration of Conformity document.
CE Conformity
This product complies with the European directives listed in the table, by fulfilling the
corresponding harmonized standards.
A copy of the Declaration of Conformity is available on request.
TitleDirective
Low Voltage Directive (LVD)2006/95/EC
Electromagnetic Compatibility (EMC) Directive2004/108/EC
10
1 Introduction
1.3 Regulatory information
ContentRequirement
Malvern Instruments
Grovewood Road
Malvern
Worcestershire
WR14 1XZ, United Kingdom
See EC Declaration of ConformityIdentity of person authorized to sign
Declaration of Conformity
Name and address of manufacturer
MicroCal iTC200 System Operating Instructions MAN0558

International standards
The standard requirements fulfilled by this product are summarized in the table below.
NotesDescriptionStandard
EN standard is
harmonized with
EU directive
2006/95/EC.
Safety requirements for electrical equip-
ment for measurement, control, and lab-
oratory us.e
EN 61010-1, IEC
61010-1, UL
61010-1, CAN/CSA
C22.2 No. 61010-1
EN standard is
harmonized with
EU directive
2004/108/EC.
Electrical equipment for measurement,
control and laboratory use - EMC require-
ments.
EN 61326-1, IEC
61326-1 (Emission
according to CISPR
11, Group 1, class
A)
CE Marking
The CE marking and the corresponding Declaration of conformity is valid for the
instrument when it is:
•used as a stand-alone unit, or
•connected to other CE marked instruments, or
•connected to other products recommended or described in the user documentation,
and
•used in the same state as it was delivered from Malvern Instruments, except for
alterations described in the user documentation.
11
1 Introduction
1.3 Regulatory information
MicroCal iTC200 System Operating InstructionsMAN0558

Instrument safety compliance
specifications
Malvern Instruments MicroCal iTC200 calorimeters carry the CUE (Canada, USA, Europe)
safety certification mark, authorized by TÜV America, a division of TÜV Süddeutschland, to
signify that:
•The instrument has been tested by an accredited certification body and meets appli-
cable Canadian electrical safety standards/requirements (CSA/SCC).
•The instrument has been tested by an NRTL (Nationally Recognized Testing Labora-
tory) and meets applicable United States electrical safety standards/requirements
(ANSI/UL).
•The instrument has been tested by a competent and notified body for applicable EU
directives and meets applicable safety standards/requirements (EN/IEC).
Regulatory compliance of
connected equipment
Any equipment connected to MicroCal iTC200 should meet the safety requirements of
EN 61010-1/IEC 61010-1, or relevant harmonized standards. Within EU, connected
equipment must be CE marked.
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1.3 Regulatory information
MicroCal iTC200 System Operating Instructions MAN0558

2 Safety instructions
About this chapter
This chapter describes safety precautions and emergency shutdown procedures for
MicroCal iTC200. The labels on the system and information regarding recycling are also
described. All users must read this chapter before using MicroCal iTC200 and observe
the safety information at all times during use.
In this chapter
This chapter contains the following sections:
See pageSection
142.1 Safety precautions
222.2 Labels
262.3 Emergency procedures
272.4 Recycling information
282.5 Declaration of Hazardous Substances (DoHS)
MicroCal iTC200 System Operating Instructions 28-9639-77 AD 13
2 Safety instructions
MicroCal iTC200 System Operating Instructions MAN0558

2.1 Safety precautions
Introduction
Before installing, operating or maintaining the system, you must be aware of the hazards
described in the user documentation. Follow the instructions provided to avoid personal
injury or damage to the equipment.
The safety precautions in this section are grouped into the following categories:
•General precautions
•Using flammable liquids
•Personal protection
•Installing and moving
•System operation
•Maintenance
General precautions
WARNING
Do not operate MicroCal iTC200 in any other way than described in
MicroCal iTC200 user documentation.
WARNING
Do not use MicroCal iTC200 if it is not working properly, nor if it has
suffered any damage, for example:
•damage to the power cord or its plug
•damage caused by dropping the equipment
•damage caused by splashing liquid onto it
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2.1 Safety precautions
MicroCal iTC200 System Operating Instructions MAN0558

WARNING
Provide proper electrical power to the instrument. This should be
100-240 VAC, 50/60 Hz, with a Ground Fault Circuit Interrupter
(GFCI). The power strip supplied with your system, contains a GFCI.
All power plugs and cords should be 3-prong, grounded cables or
outlets.
WARNING
Protective ground. MicroCal iTC200 must always be connected to
a grounded power outlet.
WARNING
In case of fire, unplug MicroCal iTC200.
WARNING
Access to power switch and power cord. The power switch must
always be easy to access. The power cord must always be easy to
disconnect.
WARNING
Use caution when using solutions near the instrument. If any liquid
is spilled on or around the instrument, unplug the instrument im-
mediately and wipe it up.
If there is any possibility that liquid may have leaked into the instru-
ment case, contact Malvern Instruments immediately. Do not plug
the instrument into any electrical outlet until the problem is resolved.
WARNING
The MicroCal iTC200 cells are constructed out of Hastelloy™. Strong
acids must be avoided.
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2 Safety instructions
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MicroCal iTC200 System Operating Instructions MAN0558

NOTICE
Electromagnetic and radio frequency interference (EMI/RFI).
Laboratory tests have shown that electromagnetic and radio fre-
quency waves may affect the performance of MicroCal iTC200.
Electromagnetic and radio frequency interference can come from
sources such as mobile phones, mobile two-way radios (such as
walkie-talkies), radio stations, TV stations, amateur radio (HAM)
transmitters, wireless computer links, microwave signals, paging
transmitters, and medium-range mobile transceivers used by
emergency vehicles. In some cases, these waves can cause unin-
tended data results.
Please refrain from all use of such equipment and leave cellular
telephones in the OFF position.
Using flammable liquids
WARNING
Fire Hazard. Before starting the system, make sure that there is
no leakage.
WARNING
A fume hood or similar ventilation system shall be installed when
flammable or noxious substances are used.
Personal protection
WARNING
Hazardous substances. When using hazardous chemical and
biological agents, take all suitable protective measures, such as
wearing protective glasses and gloves resistant to the substances
used. Follow local and/or national regulations for safe operation
and maintenance of MicroCal iTC200.
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2 Safety instructions
2.1 Safety precautions
MicroCal iTC200 System Operating Instructions MAN0558

Installing and moving
WARNING
MicroCal iTC200 must be installed and prepared by Malvern
Instruments personnel or third party authorized by Malvern
WARNING
Power cord. Only use power cords with approved plugs delivered
or approved by Malvern Instruments.
WARNING
Always plug the instrument intoaGround Fault Circuit Interrupter
(GFCI).
WARNING
Do not block the ventilation inlets or outlets on the system.
WARNING
Access to power switch and power cord. The power switch must
always be easy to access. The power cord must always be easy to
disconnect.
WARNING
Replace fuses ONLY with the same type and rating as the spare
fuses that are provided with the original shipment.
17
2 Safety instructions
2.1 Safety precautions
Instruments.
MicroCal iTC200 System Operating InstructionsMAN0558

NOTICE
Disconnect power. To prevent equipment damage, always discon-
nect power from the MicroCal iTC200 instrument before an instru-
ment module is removed or installed, or a cable is connected or
disconnected.
NOTICE
Lift the MicroCal iTC200 instrument in the upright position. Do not
use the front panel cover as a lifting handle.
System operation
WARNING
All solutions in the cells must be cooled down below 40ºC before
removal. Any higher temperature may cause the syringe to break,
and will increase the dangers of most hazardous solutions.
WARNING
Do not place containers of liquid on top of the instrument, except
those designed for the washing module. Spilled liquid is a fire,
electrical and corrosion hazard.
18 MicroCal iTC200 System Operating Instructions 28-9639-77 AD
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2.1 Safety precautions
MicroCal iTC200 System Operating Instructions MAN0558

WARNING
Methanol is highly volatile and can be hazardous to humans.
•Storage containers should be kept tightly closed.
•Methanol should always be transferred in a well-ventilated
area with no ignition sources. The operator should have protec-
tive clothing, eye protection and gloves.
•Methanol can be absorbed through the skin. Do not allow
methanol to be swallowed or to come in contact with skin or
eyes. If accidental exposure occurs, flush the affected area with
water. If methanol is swallowed, or there is significant skin or
eye exposure, seek medical help.
WARNING
Hazardous chemicals during run. When using hazardous chemi-
cals, flush the entire system tubing with distilled water, before ser-
vice and maintenance.
CAUTION
Waste tubes and containers shall be secured and sealed to prevent
accidental spillage.
NOTICE
Never allow liquid in the cells to freeze. The expansion of the liquid
can distort the cells and rupture the most critical sensor, causing
irreparable damage.
NOTICE
The MicroCal iTC200 system should always be moved in its normal
operating orientation. Other orientations will subject delicate sen-
sors inside the instrument to stress.
MicroCal iTC200 System Operating Instructions 28-9639-77 AD 19
2 Safety instructions
2.1 Safety precautions
MicroCal iTC200 System Operating Instructions MAN0558

Maintenance
WARNING
Repairs, alterations or modifications must only be carried out by a
Malvern Instruments specialist, or with explicit directions from a
Malvern Instrument technician. Removal or modification of any
cover or component could result in an unsafe or easily damaged
WARNING
Always use appropriate personal protective equipment (PPE) during
cleaning and maintenance of the equipment.
WARNING
The syringe may be contaminated with hazardous residual com-
pounds. Consult your completed Health and Safety Declarations
Form to determine if any biologically or chemically hazardous
substances have been used in the instrument. Use the appropriate
personal protective equipment (PPE) as specified in the MSDS for
those substances.
WARNING
Replace fuses ONLY with fuses of same type and rating. Several
spare fuses are provided with the original shipment and the power
receptacle is labeled with the correct type.
WARNING
Only spare parts and accessories that are approved or supplied
by Malvern Instruments may be used for maintaining or servicing
20
2 Safety instructions
2.1 Safety precautions
Instrument.
MicroCal iTC .
200
MicroCal iTC200 System Operating Instructions MAN0558