Bio-Optica AUS124 Troubleshooting guide

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
Tel. [phone] Fax Acquisti/Export +39 02.21.54.155
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AUTOMATIC STAINER AUS124
Code: 40-400-000

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
Tel. [phone] Fax Acquisti/Export +39 02.21.54.155
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Revision 160804 of 04/08/16
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USE PRECAUTIONS
Before using the instrument, read carefully the instructions and warnings contained in this
manual and keep it for further reference. They supply important indications regarding the
functions and safety for installing, using and maintaining the instrument.
Bio-Optica Milano S.p.A. cannot be held responsible for any damage caused by improper or
incorrect use and by the non-observance of any of the prescription provided in this manual
and by the safety regulations in force.
1. After unpacking, make sure that the instrument is complete and not damaged by transport.
2. Before connecting the instrument to the power supply make sure that its rating corresponds to that of the power
supply.
3. This instrument must only be used for the purpose for which it was designed, that is, as automatic stainer for
laboratory use. Any other use is to be considered improper and therefore hazardous.
4. The instrument must only be used by authorized and professionally qualified technician.
5. The electrical safety of this instrument can be guaranteed only if it is correctly connected to an efficient earth circuit
as indicated by current electrical safety regulations. It is necessary to check this fundamental safety prerequisite, and if
in doubt, ask to check the circuit. The instrument is provided with a power supply cable having 2 wires + ground tap that
have to be connected to the power supply socket.
6. Do not remove the chassis or parts of it during operation. Switch off the instrument and disconnect the power supply
cable before opening it. This operation must to be effected only by authorized and professionally qualified technician.
7. To eliminate instrument malfunctioning risks, do not work near strong magnetic fields and do not use transmitters
such as cellular phones near the instrument. In case of serious malfunctioning switch off the instrument and contact the
Technical Assistance Service.
8. All waste material, both infectious and radioactive, deriving from the appliance working cycle must be disposed in
compliance with the regulation in force.
This appliance is marked from this symbol, in compliance with EU directive 2002/96/CE regarding
electric and electronic appliances waste. This mean that the instrument, at the end of its useful life, must be collected
separately from other refuse. The user must deliver it to the special differentiated refuse collection centres, that are
predisposed by the public authority.
9. The contents of this manual is subject to change without further notice.
10. Please find enclosed the declaration of conformity.

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
Tel. [phone] Fax Acquisti/Export +39 02.21.54.155
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Revision 160804 of 04/08/16
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11. Graphic symbols indicated on the label (positioned near the instrument’s power supply socket):
Symbol for CATALOGUE NUMBER:
Symbol for SERIAL NUMBER:
Symbol for ALTERNATING CURRENT:
Symbol for FUSE:
Symbol for CONSULT THE INSTRUCTIONS:
Symbol for EC MARKING:
Symbol for IN VITRO DIAGNOSTIC-MEDICAL DEVICE:
Symbol for DISPOSAL OF ELECTRIC AND ELECTRONIC EQUIPMENT:
Symbol for DATE OF MANUFACTURE:
Symbol for MANUFACTURER:

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
Tel. [phone] Fax Acquisti/Export +39 02.21.54.155
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Revision 160804 of 04/08/16
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INDEX
1) Technical features. Page 5
2) Installation. Page 7
3) Water connections. Page 7
4) Control panel. Page 10
5) Date and hour setup. Page 11
6) Instrument’s start. Page 12
7) Dishes map and relative contents. Page 14
8) Instructions for the rotation of the dish holder disk. Page 16
9) Parameters setup and programming. Page 18
10) Alarms and alerts. Page 28
11) Maintenance and filters replacement. Page 29
12) Programmed maintenance and Instrument cleaning. Page 30
13) Main safety elements. Page 30
14) Main components. Page 30
15) Problem solving. Page 32
16) Fuses replacement. Page 33
17) Electric wiring diagram. Page 34
18) Declaration of conformity. Page 38

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
Tel. [phone] Fax Acquisti/Export +39 02.21.54.155
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1) TECHNICAL FEATURES
Carousel automatic stainer for colouring histological and cytological slides.
Dimensional features
Dimensions (WxDxH): 750x750x650 mm.
Weight: 75 Kg.
Electrical connections
Power supply: 230V~ 50/60Hz.
Power: 120 Watt.
Other connections
Water connections: Provided pipes for supplying the running water dishes:
N. 1 running water inlet pipe, internal Ø 10 mm, provided with rapid connection with
interception.
N. 1 running water outlet pipe, internal Ø 18 mm.
N. 2 neoprene pipes - internal Ø 10 mm, one metre long - provided with rapid connection
with interception (for the connection between the dishes and the instrument).
Water pressure: From 2,5 to 4 bar.
Structural features
 Painted steel chassis resistant to scratches and solvents.
On the right side are placed: N. 1 green general switch, N. 1 power supply socket with relative fuses, N. 2
connecters for pipes connecting running water dishes/instrument, N. 1 warning light for line voltage existence.
On the left side are placed: N. 1 connecter connecting running water inlet pipe, N. 1 connecter connecting
running water outlet pipe, N. 1 running water pressure regulator, N. 1 filter seat cover provided with handle.
 External cover for containing fumes.
 Basket holder cover, positioned under the external cover, provided with 24 guides for coupling one or more
baskets and 24 stainless steel covers for covering the dishes.
 Central basin for carrying waste water from wash dishes to the drain placed on the left side of the instrument.
 N. 4 adjustable rubber feet to get a perfect horizontal position.
 Aspiration/filtration system for the treatment of fumes. The instrument is provided with N. 1 active charcoal
cartridge filter specific for alcohol and xilol. Practical filter’s replacement from the left side of the instrument.
 Revolving dish holder disk to facilitate the access to the dishes placed on the back side.
 N. 2 stainless steel slide holder baskets (capacity 65 standard slides 26x76 mm).
 N. 21 reagents dishes (capacity 750 ml) in white thermoplastic material.

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
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 N. 1 dish (capacity 750 ml) in white thermoplastic material for baskets load/discharge in position 0.
 N. 2 running water dishes (capacity 750 ml) in transparent thermoplastic material.
 N. 2 independent electric valves which activate the water flow when there is the passage in running water.
 Pressure regulator to regulate the water flow.
 N. 2 lithium buffer batteries for emergency power supply in case of black-out. Instrument automatic restart when
returning the current.
Working features
 General ignition switch.
 Warning light of line voltage.
 Emergency switch for stopping all the machine functions at any time.
 Entire management of the instrument through use of single colour touch screen panel with intuitive graphics.
 Possibility of visualizing steps of programme during the execution of the cycle.
 Possibility of memorizing more than 50 different protocols of coloration, with maximum number of 30 passage
for each.
 Possibility of programming for each station: time of immersion/oscillation and dripping from 00’ : 01’’ up to 50’ :
59’’.
 Possibility of setting up in machine parameters time of pause between the stations from 00’ : 01’’ up to 50’ :
59’’. Pause time is set up by default at 00’ : 01’’.
 Possibility of changing, during the execution of the programme, the speed of oscillation, dripping and speed of
rotation.
 Possibility of programming the filter’s replacement.
 Possibility of compiling the map of the reagents loaded in machine specifying: name of reagent and its duration
with automatic date of the insertion.
 Visual and acoustic alert in case of end of use of the reagents (function - check reagents).
 Page of visualization and description of alarms accessible also during the execution of the programme.
 Possibility of stopping and restarting a programme (in case of possible controls, filling up of reagents, ecc.).
Visual and acoustic alarm in case of: cycle end, filter’s saturation, absence of current.
 Visual and acoustic alarm in case of table out of position.
 Visual and acoustic alarm for water discharge (malfunction of electric valve, obstruction discharge pipes, etc.).
 Double reset of safety in case of accidental stop.
 Possibility of visualizing the steps of the stations during the cycle.

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
Tel. [phone] Fax Acquisti/Export +39 02.21.54.155
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2) INSTALLATION
During the stainer moving, proceed cautiously in order to avoid possible damages.
Position the instrument on a level and stable working bench, free of vibrations. Make sure that on the upper part there is
space enough (ca. 30 cm) to allow the ascent/descent movements of the cover. Regulate the 4 rubber feet to get a
perfect horizontal position. Connect the instrument to the power supply socket (230V~ 50/60Hz) using the provided
cable.
Important:
 Do not use any extension or adapter and do not modify the provided cable.
 Respect the following working environmental conditions:
Room temperature between 10°C and 40°C.
Relative humidity between 30% and 75%.
Maximum temperature inlet water: 40°C.
3) WATER CONNECTIONS
Inlet running water
Connect the provided pipe ( 10 mm) between the connecter placed on the left side of the instrument and a water tap.
Outlet running water
Connect the provided pipe ( 18 mm) between the connecter placed on the left side of the instrument and the drain of
the laboratory.
Important: As the draining happens by fall, it is necessary that the pipe is positioned as linear as possibile and
downhill (the water outlet connecter of the stainer must be situated over del water outlet connecter of the laboratory).
Connection between the two running water dishes and the instrument
Connect the two provided pipes ( 10 mm) between the connecters of the running water dishes and the connecters
situated on the right side of the instrument. Choose the position of the dishes according to the main programme. Make
sure that the pipes are long enough to rotate the dishes holder disk.

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
Tel. [phone] Fax Acquisti/Export +39 02.21.54.155
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Left side of the instrument.
Right side of the instrument.
Water inlet connecter
Water outlet connecter
Pressure regulator
Filter seat
Green
general switch
Power supply
socket
Connecter
for wash
dish N. 2
Connecter for
wash dish N. 1
Warning light
for current in
line

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
Tel. [phone] Fax Acquisti/Export +39 02.21.54.155
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Adjustment of the pressure reducer valve
The pressure reducer valve regulates the water flow when the pressure of the plant is irregular or not adjustable.
Instructions for the flow adjustment:
1) In main menu touch key HAND DRIVES.
2) Enable the wash dishes touching keys OFF by WATER 1 and WATER 2.
3) Open abundantly the water.
4) Pull the knob till you hear a click and rotate it till you get the right quantity of water (clockwise to increase the flow,
anticlockwise to reduce it).
5) Finished the adjustment, block the valve by pressing the knob till you hear a click.
6) Disable the wash dishes touching keys ON by WATER 1 and WATER 2.
7) In order to return to menu, touch exit key positioned low on the left.
Menu Hand controls selection
Enable the flow of water
in the tank 1 (water1)
and in the tank 2 (water2)
Hand controls

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
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4) CONTROL PANEL
Touch screen control panel, intuitive, impermeable IP55, multilingual for the programming/modification/visualization of
the parameters of work and the control of the process of coloration.
Initial screen Language selection
Main menu side bar + task bar

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
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FUNCTIONS DESCRIPTION
REGULATIONS AND
MAINTENANCE
 SPEED REGULATION.
 USER MAINTENANCE: Access through password, reserved to Technical Assistance
Service (machine parameters).
 FILTERS MAINTENANCE: Control/updating of filter’s life.
MANUAL COMMANDS Allows to page to manual functions of machine.
REAGENTS Allows to page to dishes map and relative contents and their state.
ALARMS AND ALERTS
(check reagents) Allows to page to page of alarms and alerts for the control of the reagents..
PROGRAMME
FUNCTIONS (steps
programming,
programme monitor and
choice of the
programme)
Allows to page to programmes management and to personalized programming.
HOME KEY Allows to exit from each screen in order to return to menu.
DIAGNOSTICS Allows to observe possible malfunctions identifying the problem to communicate to
Assistance Service.
SELECTION
LANGUAGE Allows to choose up to 8 different languages.
5) SETUP DATE AND HOUR
It is possible set up date and hour both from main screen and from menu of the functions touching directly the key date
and hour and appears the screen with set date and hour.
Appears a message for confirming the modification, confirm touching key YES or exit touching key NO.
Set up the desired date and hour and confirm all the set up data touching key “CONFIRM DATE AND HOUR”.
Press exit key law on the right in order to return to menu.
Task bar menu – date and hour
selection Example : screen date and hour

User and service manual Stainer AUS124 from sn 101548475
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6) INSTRUMENT’S START
Switch on the instrument by pressing the green main switch placed on the right side of the instrument.
On display appears the initial screen and a sound signal advises that the machine is working. Effect the zero setting of
the machine.
Touch the screen to page to main menu of the functions.
Key ALARMS (placed low on the right) lightens intermittently and you can hear a sound signal.
Touch key ALLARMS to page to page of alarms.
Touch key RESET to zero the machine. The basket holder wheel will position on starting position (0).
Touch key HOME low on the right to return to main menu.
Task bar menu – alarms selection Example : screen alarms

User and service manual Stainer AUS124 from sn 101548475
Bio Optica Milano S.p.A. via San Faustino 58 I-20134 Milano
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6.1 MANUAL OPERATIONS
In manual function it is possible to manage manually all machine movements.
Hand controls screen
1 = Direction arrows for manual management of the vertical movements of the baskets.
2 = Visualizes the basket position on the dishes wheel.
3 = Activates manually the function of basket immersion with relative oscillation inside a dish. Touching key OFF/ON is
activated the function. To deactivate the immersion function, touch key OFF/ON again.
4 = Manual function for the basket moving of one position on the dishes wheel. Touching key OFF/ON once, the basket
will move of only one position. Keeping pressed key OFF/ON, the basket will continue moving from dish to dish.
5 = Touching key OFF/ON is activated the water flow in dish WATER 1.
6 = Touching key OFF/ON is activated water flow in dish WATER 2.
7 = Function that activates manually the basket dripping. Touch key OFF/ON to activate/deactivate the function.
8 = Touching key OFF/ON in any position on the dishes wheel, the basket will return to position 0.
ATTENTION: It isn’t possible to exit from manual management of movements if the machine isn’t in position of
0.

User and service manual Stainer AUS124 from sn 101548475
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7) MAP OF THE DISHES AND RELATIVE CONTENTS
Touch key REAGENTS1 and REAGENTS2 in main menu of the functions.
It is possible insert for each dish: name, date of load and minutes of use of each reagent up to 23 reagents.
The first column on the left shows the position of the dish.
The second column shows the name of the reagent.
The third column shows date and hour of insertion and happens in automatic when the timer is renewed (reagent’s
duration).
The fourth column shows the duration in minutes of the reagent.
In the fifth column the square lightens intermittently in red when the minutes of use of each single reagent are finished.
Touching the square corresponding it is possible to name the reagent and its duration.
Side bar menu – selection reagents 1 / reagents 2
Example : screen reagents 1 (1-12) and reagents 2 (13-24)

User and service manual Stainer AUS124 from sn 101548475
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Insert mode reagents in map
Example : screen reagents complete

User and service manual Stainer AUS124 from sn 101548475
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8) INSTRUCTIONS FOR THE ROTATION OF THE DISH HOLDER DISK
The swivel dish holder disk allows to accede easily to the dishes positioned on the back.
 In main menu touch key MANUAL COMMANDS.
 Touch the key shown in the following picture (arrow up) in order to bring the basket holder cover to end run in
height.
 Pull the release knob and rotate cautiously the disk in the desired position taking care to the pipes connected to
the dishes as shown in the sequence in succession.
Reporting generic reagents exhausted Detailed reporting of reagents in missing to replace
1 - Send the cover baskets at the stroke end in
height button 'UP' - screen hand controls
2 - Disables the water

User and service manual Stainer AUS124 from sn 101548475
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3 - when the machine is stopped,
identify release knob
4 - Unlatching the knob to
unlock the disc
5 - rotate the disc with caution in
desired position
6 - restore the reagents and reset the timer
(examples in red)
7 - rotate the disc carefully in the starting position
until the knob back into position.
8 - run the zeroing

User and service manual Stainer AUS124 from sn 101548475
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9) SETUP PARAMETERS AND PROGRAMMING
Menu task bar - selection program
Insert mode new programs and recall preset programs

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9.0 SETUP PARAMETERS, STEPS OF THE COLORATION AND SAVING OF A PROGRAMME
1. Page to menu low, key PROGRAMME.
2. Appears the screen of programme.
3. Proceed in STEPS PROGRAMMING. Appears the screen that shows 30 squares, one for each step to
program.
4. Starting from first key P1 (first step of programme) up on the left, set up the desired parameters for each step of
programme:
 n° position = number of the desired dish you can visualize in the MAP.
 T. immersion/oscillation = time of immersion or oscillation of the basket in a dish measured in minutes and
seconds.
 T. dripping = shows the time of dripping of a basket after the phase of immersion outside the dish, measured in
minutes and seconds.
 E.V. water 1 = activates (ON) or deactivates (OFF) the flow of running water in the dish N. 1 for the whole
duration of immersion of the basket.
 E.V. water 2 = activates (ON) or deactivates (OFF) the flow of running water in the dish N. 2 for the whole
duration of immersion of the basket.
 Set up the parameters in each step, touching on the screen the value to set up.
5. Once the parameters in relative steps are set up, it is necessary to name and save the programme. Exit from
the screen of steps setup. In oder to save the steps and relative set up parameters, touch key PROGRAMME.
Appears the screen where it is possible to manage the realized programmes.
6. The programme realized and saved is so saved and can be retrieved in every moment. A programme is loaded
in machine if appears near the words PROGRAMME low.
7. In MONITOR PROGRAMME the cycle is ready for the start.
1 - insert a new recipe 2 - save the name and number of the recipe

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3 - display the screen for programming steps 4 - program each step of the new recipe
5 - save the recipe with programmed steps
6 - recall the recipe saved 7 - start cycle