Thermo Scientific BIOShield 1000A User manual

Thermo Scientific
BIOShieldTM 1000A
Instruction Manual
50119946-f• 07/ 2020
Visit us online to register your warranty:
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WEEE Conformity
This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment
(WEEE) Directive 2012/19/EU. It is marked by the following symbol:

Centre of Emergency Preparedness and Response
Health Protection Agency
Porton Down
Salisbury
Wiltshire SP4 OJG
United Kingdom
Certificate of Containment Testing
Containment Testing of Thermo
Scientific rotor 75003603
Report No. 59-08 G
Report prepared for: Thermo Fisher
Issue Date: 15 th January 2009
Test Summary
A Thermo Scientific 75003603 contained rotor (Max speed 6,030
rpm) was supplied by Thermo Fisher and containment tested at 6,030
rpm using the method described in Annex M of EN 61010-2-020.
The rotor was shown to contain a spill when tested in triplicate.
Report Written By Report Authorised By

Thermo Scientific 75003603 BIOShield
TM
1000A i
T
Preface .......................................................................................................................................iii
Scope of Supply ........................................................................................................................iii
Precautions ...............................................................................................................................iii
Chapter 1 Rotor Specifications ..............................................................................................................1-1
Technical Data .........................................................................................................1-2
Chapter 2 Accessories .............................................................................................................................2-1
Chapter 3 AutoLockTM .............................................................................................................................3-1
Rotor Installation .....................................................................................................3-2
Removing the Rotor .................................................................................................3-3
Chapter 4 Rotor Loading ..........................................................................................................................4-1
Before a Run ............................................................................................................4-2
Proper Loading .........................................................................................................4-2
Improper Loading ....................................................................................................4-3
Maximum Loading ...................................................................................................4-3
Cycle Counter ..........................................................................................................4-4
Chapter 5 Aerosol-tight Applications ...................................................................................................5-1
Basic Principles .........................................................................................................5-2
Placing O-ring ..........................................................................................................5-2
Fill Level ...................................................................................................................5-2
Checking the Aerosol-Tightness ...............................................................................5-2
Chapter 6 Maintenance and Care ..........................................................................................................6-1
Cleaning intervalls ....................................................................................................6-2
Cleaning ...................................................................................................................6-2
Disinfection ..............................................................................................................6-3
Decontamination .....................................................................................................6-4
Autoclaving ..............................................................................................................6-5
Service of Thermo Fisher Scientific ..........................................................................6-5
RCF-values ............................................................................................................... A-1
Chemical Compatibility Chart ............................................................................. B-1
Table of Contents

C Contents
ii 75003603 BIOShield
TM
1000A Thermo Scientific

Thermo Scientific 75003603 BIOShield
TM
1000A iii
P
Preface
Before starting to use the rotor, read through these instruction manual carefully and follow the
instructions.
The information contained in these instruction manual is the property of Thermo Fisher Scientific; it
is forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the seller’s warranty.
Scope of Supply
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Precautions
In order to ensure safe operation of the BIOShieldTM 1000A , the following general safety regulations
must be followed:
•Do not remove the magnet at the rotor bottom.
•Do not use rotors which show any signs of corrosion and/or cracks.
•Use only with rotors which have been loaded properly.
•Never overload the rotor.
•Never run the rotor without lid.
Article Number Quantity Check
75003603 BIOShield
TM
1000A 1
76003500 Rubber seal grease 1
75003786 Bolt grease 1
50119946 Instruction Manual 1

P Preface
iv 75003603 BIOShield
TM
1000A Thermo Scientific
•Use only accessories which have been approved by Thermo Fisher Scientific. Exceptions to this
rule are commercially available glass or plastic centrifuge tubes, provided they have been approved
for the speed or the RCF value of the rotor.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Rotor installation: Check that the rotor is locked properly into place before operating the
centrifuge.
•Always balance the samples.
Maximum sample density at maximum speed: 1.2
g
cm
3
-----------
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

Thermo Scientific 75003603 BIOShield
TM
1000A 1-1
1
Rotor Specifications
Contents
•“Technical Data” on page 1-2

1 Rotor Specifications
Technical Data
1-2 75003603 BIOShield
TM
1000A Thermo Scientific
Technical Data
Table 1-1. 230 V, 50 / 60 Hz
Centrifuge Multifuge X3 Multifuge X3 F Megafuge 40
Catalog # 75004500 75004530 75004503
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 80 / 80 80 / 80 65 / 60
Sample heating at n
max
[° C] referred to
ambient temperature of 23-25 °C,
running time 120 minutes
12 12 8
Aerosol-tight
*
*
Tested by HPA, Porton-down, UK
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R
Catalog # 75004515 75004536 75004518
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 75 / 80 75 / 80 45 / 55
Aerosol-tight
*
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
*
Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003603 BIOShield
TM
1000A 1-3
Table 1-2. 120 V, 60 Hz
Centrifuge Multifuge X3 Multifuge X3 F Megafuge 40
Catalog # 75004501 75004531 75004504
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 120 / 80 120 / 80 70 / 60
Sample heating at n
max
[° C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
12 12 8
Aerosol-tight
*
*
Tested by HPA, Porton-down, UK
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
Centrifuge Multifuge X3R Multifuge X3 FR Megafuge 40R
Catalog # 75004516 75004537 75004519
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 115 / 80 115 / 80 120 / 75
Aerosol-tight
*
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
*
Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
1-4 75003603 BIOShield
TM
1000A Thermo Scientific
Table 1-3. Sorvall 230 V, 50 / 60Hz
Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall ST 40
Catalog # 75004505 75004532 75004509
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 80 / 80 80 / 80 65 / 60
Sample heating at n
max
[° C] referred to
ambient temperature of 23-25 °C,
running time 120 minutes
12 12 8
Aerosol-tight
*
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
*
Tested by HPA, Porton-down, UK
Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R
Catalog # 75004520 75004538 75004524
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 75 / 80 75 / 80 45 / 55
Aerosol-tight
*
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
*
Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003603 BIOShield
TM
1000A 1-5
Table 1-4. Sorvall 120 V, 60 Hz
Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall ST 40
Catalog # 75004506 75004533 75004510
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 120 / 80 120 / 80 70 / 60
Sample heating at n
max
[° C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
12 12 8
Aerosol-tight
*
*
Tested by HPA, Porton-down, UK
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R
Catalog # 75004521 75004539 75004525
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 115 / 80 115 / 80 120 / 75
Aerosol-tight
*
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
*
Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
1-6 75003603 BIOShield
TM
1000A Thermo Scientific
Table 1-5. Sorvall 100 V, 50 / 60 Hz
Centrifuge Sorvall Legend XT Sorvall Legend XF Sorvall ST 40
Catalog # 75004507 75004534 75004511
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 125 / 80 125 / 80 75 / 60
Sample heating at n
max
[° C] referred to
ambient temperature of 23-25 °C,
running time 120 minutes
12 12 8
Aerosol-tight
*
*
Tested by HPA, Porton-down, UK
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
Centrifuge Sorvall Legend XTR Sorvall Legend XFR Sorvall ST 40R
Catalog # 75004522 75004540 75004526
Weight of empty rotor [kg] 8.5 8.5 8.5
Max. cycle number 30,000 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250 4 x 250
Maximum speed n
max
[rpm] 6,000 6,000 5,300
Maximum RCF value at n
max
7,164 7,164 5,590
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90 90
Accel. / braking time [s] 115 / 90 115 / 90 100 / 75
Aerosol-tight
*
yes yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121 121
*
Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
Thermo Scientific 75003603 BIOShield
TM
1000A 1-7
Table 1-6. Thermo Scientific 230 V, 50 / 60 Hz
Centrifuge Thermo Scientific SL 40 Thermo Scientific SL 40 F
Catalog # 75004512 75004542
Weight of empty rotor [kg] 8.5 8.5
Max. cycle number 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250
Maximum speed n
max
[rpm] 5,300 6,000
Maximum RCF value at n
max
5,590 7,164
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90
Accel. / braking time [s] 65 / 60 80 / 80
Sample heating at n
max
[° C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
812
Aerosol-tight
*
yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121
*
Tested by HPA, Porton-down, UK
Centrifuge Thermo Scientific SL 40R Thermo Scientific SL 40 FR
Catalog # 75004527 75004543
Weight of empty rotor [kg] 8.5 8.5
Max. cycle number 30,000 30,000
Maximum permissible load [g] 4 x 250 4 x 250
Maximum speed n
max
[rpm] 5,300 6,000
Maximum RCF value at n
max
5,590 7,164
Max. / min. radius [cm] 17.8 / 8.2 17.8 / 8.2
Pitch angle [°] 90 90
Accel. / braking time [s] 45 / 55 75 / 80
Aerosol-tight
*
yes yes
Permissible temperature range
autoclavable (cycle number) °C
121 121
*
Tested by HPA, Porton-down, UK

1 Rotor Specifications
Technical Data
1-8 75003603 BIOShield
TM
1000A Thermo Scientific
Table 1-7. Thermo Scientific 120 V, 60 Hz
Centrifuge Thermo Scientific SL 40
Catalog # 75004513
Weight of empty rotor [kg] 8.5
Max. cycle number 30,000
Maximum permissible load [g] 4 x 250
Maximum speed n
max
[rpm] 5,300
Maximum RCF value at n
max
5,590
Max. / min. radius [cm] 17.8 / 8.2
Pitch angle [°] 90
Accel. / braking time [s] 70 / 60
Sample heating at n
max
[° C] referred to
ambient temperature of 23-25 °C, running
time 120 minutes
8
Aerosol-tight
*
*
Tested by HPA, Porton-down, UK
yes
Permissible temperature range autoclavable
(cycle number) °C
121
Centrifuge Thermo Scientific SL 40R
Catalog # 75004528
Weight of empty rotor [kg] 8.5
Max. cycle number 30,000
Maximum permissible load [g] 4 x 250
Maximum speed n
max
[rpm] 5,300
Maximum RCF value at n
max
5,590
Max. / min. radius [cm] 17.8 / 8.2
Pitch angle [°] 90
Accel. / braking time [s] 120 / 75
Aerosol-tight
*
yes
Permissible temperature range
autoclavable (cycle number) °C
121
*
Tested by HPA, Porton-down, UK

Thermo Scientific 75003603 BIOShield
TM
1000A 2-1
2
Accessories
Contents
•“Rotor data” on page 2-1
•“Accessories” on page 2-1

2 Accessories
2-2 75003603 BIOShield
TM
1000A Thermo Scientific
Rotor Specifications
* Max Speed varies depending on model of centrifuge
RCF/Radius
Maximum
Minimum
BIOShield 1000A Rotor Package
Cat. No. Description
75003603
BIOShield 1000A with buckets and sealing lid
Tube Ordering Information
Sealing Assembly Required: Adapters Required: Accessories Required:
Cat. No. Qty
/Pkg Description Cat. No. Qty /Pkg
No. of
Places
3141-0250 250 250 PP Oak Ridge Bottle 4 6,030 62.5x124 Included 4 PP Sealing 75003737 4 1 - -
3140-0250 250 250 PC OakRidge Bottle 4 6,030 62.5x124 Included 4 PP Sealing 75003737 4 1 - -
- 150 - Round Bottom Open Top
Tube - - 56.5x119 - - - 75003738 4 1 - -
76009007 100 75 Glass Bottle 10 - 44x100 - - - 75003742 4 1 - -
76009084 100 75 PP Bottle 1 6,030 45x98 - - - 75003742 4 1 - -
76009095 100 75 PC Bottle 1 6,030 45x98 - - - 75003742 4 1 - -
334959 50 50 Nunc Conical 25 6,030 30x121 Included 25 PP Sealing 75003643 4 4 - -
-50-
Round Bottom Open Top
Tube - - 35x113 - - - 75003749 4 3 - -
3139-0050 50 50 PP Oak Ridge Tube 10 6,030 29x114 Included 10 PP Sealing 75003750 4 4 - -
3138-0050 50 50 PC Oak Ridge Tube 10 6,030 29x114 Included 10 PP Sealing 75003750 4 4 - -
3114-0050 50 50 Teflon Oak Ridge Tube 2 6,030 29x114 Included 2 PP Sealing 75003750 4 4 - -
-45-
Flat or Round Bottom
Tube - - 30x113 - - - 75003750 4 4 - -
45500-30 30 25 KIMAX Glass Tube 6 6,030 24x106 - - - 75003756 4 6 - -
-25-
Universal - - 26x113 - - - 75003755 4 4 - -
366036 15 15 Nunc Conical 50 6,030 17x120 Included 50 PP Sealing 75003642 4 9 - -
- 15 - Conical Urine Tube - - 17x115 - - - 75003759 4 9 - -
- 15 - Blood Collection - - 17x109 - - - 75003767 4 16 - -
-14-
Flanged Round Bottom
Tube - - 18x113 - - - 75003758 4 9 - -
3139-0010 10 8 PP Oak Ridge Tube 10 6,030 16x82 Included 10 PP Sealing 75003767 4 16 - -
3138-0010 10 8 PC Oak Ridge Tube 10 6,030 16x82 Included 10 PP Sealing 75003767 4 16 - -
- 10 - Blood Collection - 16x110 - - - 75003767 4 16 - -
- 7 - Blood Collection - 13x100 - - - 75003768 4 20 - -
- 5 - Blood Collection - 13x75 - - - 75003768 4 20 - -
- 5 - RIA Tubes - 13x75 - - - 75003769 4 28 - -
- 1.5/2 - Conical Microtube - 11x50 - - - 75003770 4 56 - -
Dims ØxL
(mm)
Cat. No.
Tube
Vol.
(ml)
Vol.
Fill
(ml)
Description Qty.
K Factor
Net Weight (kg)
Max.
Speed
(rpm)
BIOShield 1000A Swinging Bucket Sealed Rotor
90
6,000*
Angle o
Maximum Speed (rpm)
5,392
8,5
Thermo Scientific General Purpose Rotors BIOShield 1000A
4 x 250
62.5 x 124
Capacity (ml)
Tube Dimensions (mm)
RCF (x g)
7,164
3,300
Radius (cm)
17.8
8.2

Thermo Scientific 75003603 BIOShield
TM
1000A 3-1
3
AutoLock
TM
Contents
•“Rotor Installation” on page 3-2
•“Removing the Rotor” on page 3-3

3 AutoLock
TM
Rotor Installation
3-2 75003603 BIOShield
TM
1000A Thermo Scientific
Rotor Installation
This rotor is equipped with an AutoLockTM-system.
This system is used to automatically lock the rotor to the centrifuge spindle. The rotor does not have
to be bolted onto the centrifuge spindle.
Proceed as follows:
1. Open the lid of the centrifuge and if necessary remove any dust, foreign objects or residue from
the chamber.
AutoLockTM and o-ring must be clean and undamaged.
Figure 3-1. AutoLockTM
2. Hold the rotor over the centrifuge spindle and let it slide slowly down the centrifuge spindle.
The rotor clicks automatically into place.
3. Check if the rotor is properly installed by lifting slightly on the handle. If the rotor can be pulled
up, then it must be reclamped to the centrifuge spindle.
CAUTION Unapproved or incorrectly combined accessories can cause serious damage to
the centrifuge.
CAUTION Do not force the rotor onto the centrifuge spindle.
If the rotor is very light, then it may be necessary to press it onto the centrifuge spindle
with a small amount of pressure.
WARNING If the rotor cannot be properly locked in place after several attempts, then the
AutoLockTM is defective and you are not permitted to operate the rotor.
Check for any damage to the rotor, damaged rotors must not be used.
Keep the hub area clear of objects.
AutoLockTM
O-ring