Meiji Techno MT6200ECW User manual

MEIJI
TECt-I\to
MT6OOO
Series Microscope
Instruction Manual

Table
of Contents
11lMicroscopeFeatures
12GeneraSalely
Guideines
13Waming/Caution
symbolsusedinthlsmanual
14lntendedProduclUseStalemenl
1.5ProductSafelyInlomation
-Handlinglhemicroscope
1.6WatrantySlalemenl
2.0The
MicroscopeandlsComponenls
2.4
AdjuslinqnterplpillaryDislance
2.5Fluorescence
FillersInformation
2.6UVLighl
SafelyConsideratons
3.0MicroscopeOperalon
3.1IncidentLightOperalion
- Epi-Fluorescence
32TransmiftedLlqhlope6ton- Brghfeld
3,3
InstallngPhaseConlrastComponenls
3.4TransmilredLlghlOpeation
- PhasecontEsl
3.5
Pholomicographywth35mmSLRand
DigitalSLRCame€s
3.6Pholomcbgraphy
wilhDigilalStillCameEs
3.7Connecling
aVideoorOtherCameElhatusesa C lype
mounl
4.0Malnlenanceand
CLeaning
5.1Replacinglh6
mainsflseon lhemic.os@pe
5
2InlegratedTEnsmltedLghtdoes
nolwork
5.3Replacinglhe6V30W
HalogenLighl
5.4Fluo€scence
LamPdoesnotwork
5.5Replaclng
lheFllorescenceLamp
7.0Packinq
andTranspon
80AccessonesandReplacements
Parls
9.0TechnicalDescnpiions
100PhtsicalDimensions

M76000 SeriesBiological
Microscope
1.0Introduction
TbeMeiji TechnoMT6000Senesbiologicalni(oscoles
opeFtionh achicvcdlhrcushergononic
plrccbcnl
ofrhe
16rusein
producdon,
cducalion
dd clinicrl.pplicalions.
haveamodem
coopet
desirrr.
Eas,
nicrcscope
controkm inSilem ideal
MeijisMT6000Scricsis
qell
sitedfora
uide
valiclyofroltineudclinicalapplicalions$ch
asc€llobsnation.cpi-fluoEwnceobsetuationas
*clls hishtwlunon vido nicro*opy.
TheMT6000SedesMico$oFs otre.cdsp,distonion-free,hishEolurio. id.g6 i. nulriple
mod6ofop€ntion.ContEslm€thodsavailablcincludc:Bnsntfeld,Dattield,PhaeContEsr,
Sin lePolrrizariondd Ebi-FluoEsnce.
1.1
MicroscoDe
Features
CADDosign€dFrameandOpti6
Slin CoftpaclF@lpdnl
Infniry
comcled oplical system
N4 andidlpovedSemiApoPlanObj6ctiv6
Ce6micCoaledClinacalFlalTop5169€wilhoplionalspecimenhold€.
LowPo6llion€d
ErgonomicCoarlalFocus Contrcls
SmoothOp66tingErgonomicOuinlupieNosepie6e
OptionalCllni@lSp€cim€nHold€r
1ltA676)
Bdghtfi€ld/Darkflold,PhaseContrasl,Simpl€PolarizedLighiandEpl.
FluoEs€ncoObseryalionlModesavailabl6
Inl€g€t€d30{atl HalogenTENnittsd llluminalionSyslomon Bightli€lda.d
PhaseConlrastModels
Modula.SixPosnionFluoacerce FilterModuleshh 3fillersels includ6d
Abb€,Z6mik. PhaseandDa*fi€ld Cond€ns daaable
Sied€nlopllypeBinocularandTnnocularViewingHeads
Supfi WldeHighEy€point
Ey€plsc€s
AulomalicVoltageSensingPN€r Supplyforkansmiltedlighlwithdetachable
pow6r
6ord
(FL.PWJ
Epi-fluoresc6nc€PowerSupplyfor115Vor220l240V)
FL-Ll-lJFluorcsc6ntLamphous€accBpts100WMercurylanp
WideRang€ofFihe6andA@6ssori6s

1.2Ge erulSafe, Guidelines
ThisDfludconrainsnnpona safetyinsrocrtu.satrdinfomalionconcenrirSr[cin$llrlon.
openrion
rndnrinrcnmceofrheMeijiT.ch.oMT60O0Sdns
biologralmicmscopcs.
Thisnmualshouldbcrc cdclullybefole
ensuE
srlcopcrdiotrthcuscrnui rcadand
anyiuc'nprs mde toopcdt this
cquipmcnl.To
dhdR b rll.trhe didrionspul
lonhinthis
MeijiTechn.
ptuduc(
rrcdesisndd
tbrsaltopcnln)n
undernomdopemlnrgcondni.ns.The
in!tunem.ndacccssoncsde$nbedinrhnnanudlhavebeenbuiltmd
lesrcdrccordinsIo
indusqsafelysknd.dsaorclccloniclabomrorynrsrrumc.rslnconcrusrscornon,
conlomde b opcririns iostocrionscan
c * pcmnal
injuryordamagero
$ripmenr
or
Kep $is manualn.n your inshmenr
for
easyNltruncc
1.3Warning/CoutionSymbolsUsedin lhisManual
Younue bcawarco,rll srllryi$uesNhen
you
ineallrndopenterlisnicosopc system.
SetrGl NminS rndcaurionsFbols arclisrddbclo$.Th* slmbols@ us.nrhou8lmll rhis
inntudionmdual.Foryoursafcly-besureb lollosnllinstrucrioBassirtd wirhthe
srmbols
lEdiGEil oi..g*arnern,rrucrionsmnkcdwi$inissynbormayrcrdr,scnousbod,ry
EtsAi,,'||d Opcar'unJ
\m'1A:rirlurcrooncdruequ'pm.nrp6p J
TrJN.ulrn
Awrmrrnd Po$lblccl*bcrl lhocl hrzrd cr'sts
l@ oi".gu.oingi'dtucrionsmdrcdeirh lhNs)mbot
may
rcadroscriousinjuryor
[sHEl "^'an rorn"arorhotsurti'ccs.
Risk
ofbumsorsemusmrurrr
* XdE I rr,.,y.b"r doicraresrccbic'tnoro.
pbducr
rp

1.5ProductSafevInlotmorion- lheMicroscope
AreentxC1;..;".."o0" snori.r€rded|ob. $.d inrheiinediare viciniryol*,rer oi a
waler
ouderorplac.d indy laaaon{heE *arr naypd.hc rheinstrunnl.Wltcrp.netatim
nayEsnltinehtdcal sh@t ordealh.Chme onlyasuitrblemvionlMt for
}tur micrcscope.
1.4InrendedPrcductUse
Slatement
Pro.luct Disclaimer:-|hrts
paducr
k desiSned
dd intend€dforus only6 abiologicrl
nicoscopesystcm.Modiryin8lhisinsEumnliney eayforuseinanysituaionodcrlhanlhe
orisinalandinknded
product
design
willrutonaic.ll,
void$e
walmty.lrnoewnlshrllMeiji
Ten.obeliableromypeMnfordy iicidenul,indiEdorconequenlirl
drnd8cs,.rhineout
oforincoonccrion
wilhtheueor
pedomance
ofamodi6edorallered
product.
Donotsubjcdthenico$ope lo dtEm lmpeEiure fluctoations.
Erlremelenpcmtur€cbeges
nay leadloo.doffrtion witnind€ micnscopcwnicnmayeuh indamasetothcoplic.l and
Dis6sebblyofthcinstrumolnay6ult inelclricll shocLinjuryordea$,equipn.ntdanase.
lossofumnry covcmseoroay cdre od.r p.i.nii.l h&ard6us
6nsgl!dc6.
Al*a)5 tun oll rie po*er switch
anddb.on@ctthc@id 6on rh€
po*d supplywhenEpl&ing
iuses,comcring ordi$omcrine *irins, doinssmEl nrintmec€ orEpldins lhc
Use
o.ly thcs!.cified po*er @tdin aweuEDundcd
ecket. Do.or r$ in $ rnSmunded
power
Ec€pllcleorin css wn E lneEisabdt in thc
sound corduclorordflage tothceLcrical
Handling
Onlytus6oarhc
+(ificd lTc od raringaElobcueds Eplammls. S*nchof thc
Dovcr
dd dnconneclrhc
Dosq@rdbefoeapl&irg tues,Useofa no.{ofplist tusenayFslt in
elatricrl she* or
3.vft dmge your.quipm.nt.
Thelampandlamphouebeconeexr€melyhotdunnsondafterop@tion,Donot
ple. .ny
highly,lannableorvolatilemarerialclosrodr lqnp-houeduringorai.r op.otion.Dohol
louchthelmp ho!s.o!altempt to€place
thebulbfor!t l.dl l0 nirulesad.rlhelnit bs bcm
lumedofor ibjurynay6ul|
tAwaixrx
DONOTOPERATEUNLESSTIIEUNITISPROPERLYCROUNDED!

Do,or obsdcr th. rn ve sonthclmp-ho6c orpowcr
supply.Th€lmp housingdd posd
strpply
shouldnusl belc.ted atl€61 l0 cn (4in€hcs)awayfmm lh€wauor dy conbustible
Modi9ing &e islrunerl inanywayorunaulhorizcdotlcmplslodisdsnble oru* $e
in$nmenlforapplicalioDsolh€rlhenitsinlendcddcsisnwillaulon.ticrllyvoidlhewmty.
1,6 Wanon,t Statement
MeijiTecbnowamntsthisDFductasoinstdetcclsinmolcialondorworknansbiptorlhelireotlhe
instroment
6on tlc dalcoflheoneihal
purchBc
totheodginal
purchaser.
Me'jiTechnowillEponor
replace,ar
nsopiion,dyinslrumenlwhich!ndc!nom.lcondidons
ofusedd seryice
proves
lobe
dcfeciiv€in naterialorwo.tndship, Nocharsc
qill bemad€aorlabo.ornrlerirh wilh Especr1o
dcfNiscoveEdbylhis
wmnly, povided
allEpan*o isdoncbyMcijiTecnno.
Thk wmly doesnolcov€rexpmscsincuftd intherenovalorrcinslauationof dy inst@e oi
inshftdls, {h€lhcr ornolpnrcn dea*lire. RcploccmolorEpriB tumishedsdd thiswamry ac
subjecrlo rhesme remsmd corditionsofthcori8inalwmnry. This*a@ty supeFed6dy ode.
*afuty dd is$bjer 1od. followinsr.ms dd condilionsi
w.mly ofM€ii T6hro sprcduct exhdr lo tlc onsinalpumher ollh€ poducl ald ismt
WARRANT|DAMNON
Meti T€chnowmnts this
prDduct
asainstdclccrsin matn.l Mdor *o&,fuship rf rhelir€or
lheinslDnd non lncdar.6fod4.al purche |o dc orisinalpuEher. Tn€eblrical
OWNER'SREG]STR.IIION CAAD
Retum
ollhe offi.! sEsistralioncad bythcoriSinal
puEh.ser
wiInir rd {l0) darsaner$e
onsiral purch6eisacondition
p@dent |o@vcBscunderthiswlmry. Meiji Tcchnowill ar
irsoptionaccept
writrdpnolofp!rche tom lh.originalo*nerin
lieuofaprducl
EXCLTJSI ONSAND LIMI fATIONS
Specifically
excludedfrontniswaranly.rcfrihEscalsedbyabuse!neglecr,misuse,impopq
opontion,nomalNqr, accidenl,inprcpefhaiolcndccornodi6colioDsofANYlr?e.Tbis
wmty d@sDol@verEpairorepl&chcnt whsrcmmal uschs cxnaustedtnelile ofap.n
orinstrunenl.A11m4hmidl dcvica n.d pcriodicp.rG replacenentdd seryicelo pefom
*€ll. SeRie lif€ of d inllru@r isdepqdenlupon|hecar n rcceivesfld rheonditions uder
shich it hrsioopeale.h noev€ntshallMeiji Tcchnobeliablelor incidaral [e-mail]

SERVICE
Toobrainsctuice
uoderrhis
wamnty,pleaeconlacl
MerjiTelnodiedy nd ak forInc
PnduclScaiccDepment. Sbt de.alurcol$c pFblen,
modelddsrial numberofthc
iNrrunEnr.drt ofpurhdc dd locarion andn.nc ofthe dislributorthcinstrument *as
purchased
r'rcm.Anervenlicationoiwalaly reBGLarioqMejjiT{h.o *iU isie amun
aulholiz:tbn numbc.CuslomcrmrylbcnFirm lh pbducrposl,gepEpaidandinsucdto rhc
h nDn insl'nccs,reqredsforwaifurysedicewillbepefomed
ina
Dompr
androuri.e
mamcrandndnhddi* *ill bcaluded inaaaotublc p€riod
oflimc oralMe'ji Tshno s
convdi.ncc.In
emecd6, requcstsforva!fuly scryicec Ec.ihrdwhich@ nor
jusilicd ln
rhr..J^r.. McijiT(hno trillnovid. u expldolionlornon-wdmtracr'on
Tberedsollhis wamry maynotb€vdiedbyanypc6on,whedrerornol plrponin8
ro
rtprtenloractonbehalfofMeijiTechro.Thelinitld lifelinewmly pmlided
n inlicuor
anyandallw.r&n6, expBsd orinplied, vncdEr 6r ndhdhbilny or 6be$ iy !
pdicularpurpos
o.o6eNis. Liabilityforcons.quadslddses u.dcrey, mdallwamnries
aEexcludcdb theexrcnr.xcl$io.se Fmnted bylav. Thas*afuty gives
'ou spcciliclcsal
nglrs md youmayalsohaveolhcrrkhls Bhichv6y 6on strtetoddc. Thiswmnrt s.rsronh
lbecuslonrcrsexcltrsiverencdy,enhEspeclrodefcctive prcducls.
Thislinn€dwomnlysnall
beconc
nulldt voidinihecvoroaaviolalionollhepblisio.eofrhis
liniGd
w@nty.

2-0TheMicroscope
and
Thcimage
belosdesienales
$enainits Cotflponents comfonens
ofl[c MT6000
10
J
1
2
4
3
5
6
7
'13
\$
t-._
r Eyspioces
10x(lsxand20xavaiabe
asai opijon)
2. sedenbpfBnodarllead,30" ncrnarion
3 MT-FLModu
arSixPoslion
Fu
4. Eaonomicouinruple
Nosspiacowilh
semiApooblgcws
5. c€ramac.ared FlalTopslase
6 Abb€
NA125Condenser
7 Fred risForTEnsihed
LEhlo066iion
3 Inl€gratodlluminabr6v30w
L FL'LHJ
Epi-Fuorescence
Lamphousa
10 FL
PWJEplFruore$ene
Po@rSuppLy
11 Lampcentering
conibrs
12 Erconomic
drcpdom
coaMrsbg€contos
r3.Eaondmiccoa6eand
FinsFocus
Knobs
1.4.TE.snihed
LighlB'ishlnas
c.nltu
15.Porer swtch For
rEnsmiri.d Loht opeEr on
16 Incid€nlFeldand
Ape'lu€rrsconimLs
13 Focusrenson
Adlusheni(
ut625mma
lenw€rch)
20.Fild T€yonlopofi uminalor
21.CondenserRack
Heqhi
Adjush6iiKnob
22 AdjGlabrestury
siopforsLsehe
shr
iml
23 Focustro
co eciorl..s knob
- /11
,/
9
-'to E
17

2.I Itstsllation Site
Th. mico&opesnouB
b. op.6ld in! oomwith6 liftl. dN1 a pE lic.Uy
p.sible.
Ke.p
,!ur insfuMt aw6yfrcmsolvents,chenhll tuhesed *esirc hunidity.Alsot yto
avoidbigswinssinsbicnl LnpcEtiE, di@t sunlight&d vibEtiona rhcyc6 !frect
mc$u@di! rnd[email protected] p.rfoMe.
Opor.{ng AmblenlCondftlons
T.dp.d|rtro: l0-36'C(50 !r6.8'F)
Rda{w Humidlty:0- 80%!p io30'c
(86'F)
2.2Unpacking
Pbe cnet yN p..ting dip roiM |nalall nddids E ptgL Kcp . @pyforrod
@ord! $ lbatyo! t.E dEpopd i!&lmlioo sho odqirg !e cquipMl ordditrE
EpbdMr p.nsorlcasi* orwha callirS torrehricd sulldt PLe h.tc $E rh rc
3nall pie6 orpatts e lcft ir lh. peting mlcri.l, K.cp th. p&kilg mciials ir as.f. pl.c.
for $c purp$eofslodg. .nd tulponi4 tn niqro!@pemd ils rcce$onq.
lLarrEno Abid roqching
ihcsbc. ofoFiel onpddc auchI ldE. 6lt B .rd
gl^s 3uriG. E@ v..y s.ll tr.es ofp.r.pi6tion d fngq oit cu @md. lhes!r6.6 of
opticsi! r dori F iodoftin..

2.3Microscope
SetUp
sr li6rslcp,rcbovdallcohtonenliliomlhc
nrippinsconlain$Mdremolc$epackhg
m{rcrirls.Salcrheco.bnErrnd
p*king i[ I dry
llhccrbemicmscopeliamcdri srablewo
Lo.scn
rhecllmpscEwonftchicnscopclinb
.id inirll rhcEpiFluort{cncc
Module
(MT-FL)
oDb$c micros.opcbody.ndrightfldogtr
llE
clamp$FNsccurelywhilethenodulcisin
lhe
LooscnlheclaDpscrcwondrcbpotthc M'LFL
E FluoresccnccModulewiii thchexagotrrl
scNsdri!€rslrplicswirhrbdnicrcscopc
ed
inslall$e pnsm
housofrhc
vicwinAlDrd.
Re
ri8hrehrhcs.ts.nw bys&cw
dnvershilcfic
Dn$nhouse
isir cotr€cl
poriinin
Then,iostall
rlt binoculrron
rhe
pnsm
houscdd securul,
righlcnir
$ilhrhrsupplie'lll.. kcr.
InskllrheFL-LtlJlanphousconrheEpi
rluoNenccModulcbyirscninsthcBarclof
rhclrrnphouscinroddofthc Modulewlilc
bcire
Auided
bt rh.Guid.Pr ondEend
oirhc
Module.ndrilhrirbyrhac
sct$Ewsiound
lhcb.ftl wnhsl')'lied Uen
kcy.
lnnalllhetlo crcpiees
(MABl7)
byslidins
thcminrotheheadmdth.nhsrallrheDbhcr
crcshieldsontit ofeacheycpicce.fhc
cy.piec6forMT6m0Scricsrr.r DIN
3l.nd.rd30nmdirb.t.r.
l
5.
l

3. ReDovedreobjectives
6omthenobjeclivccascs
whilebeingcarelirlnortoiouchanypanoftheoprics
Then,screweach.bleclive
inloanosepieceopening
alterE6ovi.Beachnosepisedusr
plug.Insallthen
increnenlallyorinorierolpower(eE
10,20,40
7 Connetrhepowercable
lion IncFL-IWJ
fluorescencc
po{eisupplyvialhepower$cketon
rhercarofrhcEpi-Fluore$qceModule.
9. Pluga
powercordintothenicbscopeandlheolhe!
endinbagroundedoullel
6aaurroil
cord
\hould
only
hcplqgcd inrur khosb
grounded
oullc.
Conlacl
your
facilitiestechnicim
iryouaeunsureolyournainsouilelstalus
A siBpleoullet
rcst$cs bcu$d1ovdi8 conectoutletpdlariry
and$cpiescnccofa
gounded
circuit
Il noo$ft rccc$oris arc
aoilg lobeinsralled,
theinsirnent isnowreadyIb! us
2.4AdjustingInteryupillary Distance
TheInterpupillalyDishnce
iscssentiallyrbedislanebclw€e.
your
iwo
pupils
expEss€d
indillinercn.whensctcoredy,one
willseeon€uilom round6eldofvies or
FOv Theadjusbent
n nadcbysinply
pullin8
apanorpushing
togerherrheey€rubes
unrilauniromround6cldisachieled.Makenoleofllrenunber
Tr\eror'h". F",nghed
so
Jou.u reped.he.e.rg ldrel
whenyouplace
a
uecinen
onrheslagc,
scr
il inlofocuswiti the
nghrelr wbiledurlen eyeiscloscd.Oncedrei8htsideisiniocus
byusing$c nnelocusknobonlhenricroscope,open
yourkn eye
aMuserhedioplerouthelefleyelube
robrlnglhelellsdeinfocus.
Oncelhhh done,fienicoscope
isadjuscdb nrnuserOtheruse6
anddifierenlfocusine
db,lnies.
-+*
+

2.5FluorcscenceFilter Information
Theepi-fluortscdceequippedMT6000includesfi. lollowingtwofiltersols:
PubiBhod
ChFmaTehnologyCoD,
1t00tv2Bluo
rvd.r.nqrh (nh)
11002v2Gr...
FillorsdtP. oh.nc. So6cm€dons:
I

2,6UVLightSafeUConsiderati.,,ts
llilil cnsiryDcarclaDpshivcsubrrialUvlithr.otrlcDrinrhciroulpur.TheUS.Nlrion!l
hr$i1trrclirOccuparionalS.feryalrdllcalrh
(Nl()Sll)
rccoNncndsrharcxposuEloUVenergy
hr(,nrn)llcd andInncd6 drcb .spm.ricilly
!ossihlc
E\t surcroUVndiahonercnforrcrlbn.fpcndlsolr'mrcrnhehzrdous Thepdrcnral
drmrlcdcpcndsone\poiurc!nc. rhcrjtc olUV hlhrandrhcindiidualssnsnniryroUV
LiVl1!hrcs sunbunLontr.nnciposurcMr.sulr:nkxsofskinelanicnyiniriallyaM
^bs.aliorofUvlishtbylheeyes will.ru\c idl m ionolrhccommcalled phoro
ken'isis
Connnudcxpo\urecmleadrorhclbmdni ol.daric( onrhc
€ye
lens
Tlturcrbrc.theloll.wingsaferyconsidcrnfs sl$Lldhc
hkcn
veryseriously:
. LnDiln.cosloftcaswhcrcUv Lishlisprssorl
. Po(waningsignsr rhc.rc.whcrclhoL{ttritrhdtrr
. Alwayssedprotdti!e cyewcrrrid Ck)vcs.
. Bcsurey.$ams andncckducovctod.
. Norcrdindly laolartlr lr8hr$trrcc.
' closgonrhcIighrsourccNirhrhcrilr.rnidcrlrhcr

3.0MicroscopcOpetutio
tr
Otrc lheoict66pc ha ba Ftlp idit edtilg lqlio pil! allof th. @EOoMtt 6Gdly
ilnilhd, n isErdy 6r @
YN MT6000tu. pFili@ iDllrmat ddigr.d io ler oli6lire. AlwlF hsdk tlrr
mic@pe wi0' c@ ed woid abflpt borio!, vib.{ion 6d snoct'
Forlh. EpiFletlte@ L@pho!s., ihc folloqing pld D!r$.6 e th. ONLY [email protected]
r@l@l bulbtusd withFt PwJPowsupply:
"*-@tsrrl:
Fo.
d. tunid.d d nEidol liell, do @tild.ll uy b'rlb
itr
you irtuDot odE tnr |n. ms
ddisrd€dbyMcijiTchm:
naTvstw H"r"s""
AlwaFdi*@ct ([email protected] nw lh. D*t ofO. DiclsoF st r@l b.n'au*4 d
r[d cl@ilg yqr bleltFt dw[6 otti.8 dryttp.iE
GruTlota
avotd Dbmtntttng
N.E aftcnPttodbrutL lheirslMl, Thi! siU voa you s.ruty ed cdld Po$iblylsd
rolle iNirulMt e l@!Erpdtldjng Murdy,
Fd InbsEbd Tddli.d lllunhdor Mod€l!MI6200,
T2

3,1Inci.lentLight Operution
-Epi-Fluorcscence
Forviewinsort€nspatonlobiecls
trsin8incidenl-ligbt0uoEsence,veEcomnend
to6m oake
rod trnrdlddrurrcnaunnsbnshrleid
rit\ rtu. nr.'lI
gh.
L Toslarrep!fluorescence,
swirchonlhc
powersupplylo
lhelarnpbousemdallowthe
lamlrrotchleveopemti.g
rempmture
(openring
tenpcraluieisachievedwhenlhe
powersupplt
seulesonlhc
propcropeElingvolraae.
see
your
scperateopemtionsManualfornoE details.)
2 Openrhelightsdpleadingtothclamphouse.
3.Movcfllcr bl@kintothelishtbdth
pa$.
4 Plrcethel0xobjeclvcctrrcntlyinthelighr
pa$
5 Placeash*tofwhire
paperon
thcspccimenstage.
6.Obserue
ifthe arc inase isclearly
piojecled
andcenter€din
theli8lilspotasshoMinFisuicx.(lfnot,pDceedtothe
seclionintheLanDhouseMoual
descnbingMercuryLamp
Figure
X
a Uppertnob novesl,heMercury
ladp
b.Lowdrknobmovs lhemercurylabp
c.Focusingcoll*iorlds knob.
? Renovethe
paperand
re-inslllldreobjective.
3 Positionaknown0uor€scinssp*imenonlhesbge.
9.
Using
aloserpowd
objecrive
likellielox,cbekto se iftheimaseisilluminaledevenly.Ir
not,adjustrhe
*tinB\ on
theFLLHJhouscifnccdcn.
The
tsoelectmdesoflhearcco beseen
inrheextnsionoftheplmeoalhcdnchatgearc.Reler
lolheLanphoueManuallortunh€rinfoijnationandadjushent
insLacrions.

P6lbL lmld..t Ltsh rl@r*.N OFndorl PbbleDr
lfnomal ldjufinmll G nol g.rrirS lner$ull! you.xFct, chek th.e:
. Wql 0!otE$@ e idg. inrosity: spdiMs ihpDpdly 3torldorrm old
. R pid f.ding ofspci@r i,c.:ry6iM k lndobldhcd
. Uloei& or.mmw lilta coDbiolid
. NuEcricallFrlN ofobjetiv* loolow
. Eycpi.q.bain adonroohid
. F ili.s o!oldDCc bnp
. AEbimtliCr d'r@ r@blirhl
. S€@tl.ryliglt du.toGflcclio!,l codclq
. ljw 6ntar ir!c! du.iodciLtiD tedwiln loowid.
. Utrlp.cin dslaidlC
. Moulilg ncdim isfluoEeinc
. A!!o-0uotqe@ ofobjetir orinmGion oil
MTGOooEpl.Fluor*c.nc€Ob.iectlvaa
I aaa ls.trl&. FhrdE .r Floxdl-ffa, NA 0!O,,o. rq,(d,
: x^La lsdnl &o Fhn&hrnat F20X oDl-w., trA 0,65,sD. rb,{-i,n) I
lE*FrdI-t oqa.dr- - s..;Ano er.d'-"r drc** -'-l
xa1-ls".r Ap"Fr.*i'*;"i F4ox
..lrcrio,re,o.oz.
"o.".
*. (*-,xAl4a ls€nl&o Fl.MchM.i F4ox.6j.ct6, NA:0,32,wo.0.h,rlftd' l
--ifrrls*i6iEii**"rrroox"r "d"-"w".
r,rii.z-i."o.",^.,-"-' l

3,2Trunsnim:d Light Operutiott
- Brightjield
I
5.
Fiur,scryouiinrc.Pupillarydisrrncconrhc
micrcscopecrcrlb.sby
pullins
rhemaFn Dr
pushins
Ihcn clo*r rordhcr b ft rour .ycs
Wtenser
corectly.o.e
*ill see one
unifomrcund
fieldofriew Mrkcnoteofthedisrocc$uing
vhd adjuscdb rourliking so
touc.nl.rf
Tumonrbe
nictrxcoFpowerssitcbwhich is
locarcdassbownd nghtAdjusinedrcbnglrhes
dcsiEdisdotrcRi lbc
veiable
brishtncssconhl
Ma*esuEdc 6cldinsdiapimgmisop.ncdallthe
wayro
$c nsh (rclarc clstaise) b nan.Rcl$lo
Werecdn$cnd
dalyouinniauyusc ! lpucimcn
$alnasmcasolhigbed lowcorlms.Pla.erhdr
selet alow&|lower"scmi.g objsrilc likc
the
4Xorrh.IoX 16lindrhemr ofinrcGronrh.
sDeimeo
qlickly Bcsurelheobjectivc
''clickr"
inbtl&c wh€nyoutun rheobj$rivcnoscpirce.
By
usinslheco!6candfinefocusknobsshownabovc,adjuiyou!spucinen inloaocus
wirbyourkneycclosed
(lookins
onlydnlu8! lhcri8hleyelube).Wnhfic srccimenin
focus,cloedEnshlcye
rcujustused
!M opcnlhehn.Irthc spcnbenisourollbcus
for
the
kn ey€,sdjusrlhediopleronihcL.yctubcuhrilthclbcusincomcl Th.
nicoscop. isnowad_iusedlorthisuscr.feus'na eyetnbesallowstrsroconpensateirr
usu lhalwearcororivc rtr le.*s. lf yo! weai
BI6es. y6unay s!.1 todftore rhe
TbefieldinsdiaphBgmlocatedonthccondcn*rcmbe
sropp€ddownorclo$i
sonewha!to
givc
lncobseryationofyou!sp.cimchnoE orl€ssconmnoiresolving
powe!
SioppingdoMth€diaphm8mdccrc$esfteturionandbrighlncssburincEases
imageconlruslrnddcplhollocus.

PosibLBrl[htnrld Ilodc Oprmlion,l
P.0blcns
li.omaladjushenlsare.olsclli.slheresulrsyoucxpecl,checklosceifrhesccotrdtridNcrist:
. Incorctcondener/obj.cti!.combinari0
bcir-!usd
. IncomcrcomponflG
inndvenenrlyi.srrll.{
. Dinyo!snudged
oprics
MT6000S6rl€s
u PlanachromatBrightlieldObjectives
Brlghtli.ldObjecoves
- U.Pl.n
MAt30 u Pr..ehbtui r.5x obl€cliENA
uPramchrcmd4xobj€cit..NA0
U
Planachrcmarl0Xobl€cr€,NAi0
UPlanachomal20x,
objscriwNA
U
Planachmmdaoxobielta,NA:0
[^!35 UPran.d6mar50xobj6us.NA0
uPranad'rcnd100xoroq*M. N
3.3Trunsuitte.l
HalogenLight Operution
- Phase
Co tast
PhaeconrmsGa
veryuefulr.chnqueforhiSh..onhs
ima8esof unnrincdo!rFnsp4nr specine.s
moumcdon
Al6s
J'd * Phr,r
( Jnrminodrl. nJ'uRapo*crlullow haloscn
lrrp fo rr'rhnAhrphae.onr.Fr iTzgerliof ThcVT600n
\ene.llr{ r,,nrm{
Vodel..,'1.a
rp
" h n n0riuL
lox.
20X,
a0Xr"i "n\ | .Pldnl'lJ{cot'te. ve.'rJ 7(milePl-^'
I lnsrauthcph*cconsr
obj.ctivesinrothenos.?Eccdd
inralllnczcmikcPhase
contasrcondcnsrin
pl.cc
ofihe
slandardAhbcCondenscrbt
bwcinBlhecondcnscrEckand
loo,e1'ng'hr.l:frp-
ttr.\' tr1.{'ig\rbnJ
'lrlJiJilPoL r\c
2.RenoE
tJrcnghrhmdeyepi.rcandiosen
lhc.cntnng
r€lespc Thcn,bratelhe
l0xobjcriveimo
plNc
alonSwnhlne
coftspondirg l0X'20X
cond$sf dnnulus.
LFocusihcccnknnstlcscotcwnilclookinsrhnushnuorilrh.
lis:hrdd drk nnsssec.are shaDlocus
4 Ifrhor trliaEnorcenrerd
asshominFi$trcl.tnovelhc
Lnu'
.d
p.Fr.rdlldmrl'd ui'nrnr'ne\onrhLb1rcmoifte
zcnike
nhasccondenscrwilh
yotrr
finse6lo jusr the
lhrse
c.nd
objccliveosshownalnght
(@)
(o)
rd\c'Jnnulus{r r!,'.h
phsc

5.Rcpcatthe
poc€rs
lorthecn ofrhepositionsbcing
su€ro
marchlhcdnulustorhcrightobjecrive.
6.Rcflrovcrhecerlon.sleleepc andr ituralllhc ctdpiec.
Thenico$ope n nowpop€rly serforph6e conhn
Ph6e
inagcsNillpborognphrhdappcarrhcirben
shd rne
sEcn
inlerfeFncefiher546nn
(MA86l/05)
is
achnmaricobjccrivBaresphcic.llycoreclcnfor
€Ecn
lhc
ereo wrvelehgrh +ehm b*t.
plded ido fic li8hrpad sincc
UEnt&d thchu'm eye
picks
up
r Wrcngpha\cunulNbeingu*d
. Ph6ednulls mt coteEdcomctly.
. wmnecond.nsi,slalled.
. ApenuEdidphlagm
isconplcrclycloscd
. Halos@undrhcoudines
ofdelaikft opricalanifrc6whichftat obscurcdelailsolyour
\pR
,mcnTh'
t6 dbom l|m ruo olpha\c
!on6t microeopy
. Sina
ph6e
anuli linit lhroumencal
npcnurc
oathe
opriolsFren, in.$ €slurion
fraysullcrsomewhat.
. ltrhespecincnbeingohscrvL{islootbick,phassbitin8willdisbndrcimagedelails.
Pha$ ContraslObjectivostor MT6000S€ries
(Optional)
Po$lbhPhtscCotrlnslMod.Op.rrrioml Probkms
Ilnonnrladjusimonlsrrcnor
setrins
thcresuhs
you
cxp{| checkrhcse:
Ph.s. Contnlt Obj.ctiv.! - Pl.n.chbrul dmycqe'd F'rhh
ilA330 UPlanPha$ 10xobiacri€
[A310 u PranPha$ 20x obiecri€
MAtl u Pr.nPha$
40X
obj.cri€.
NA:0
65 wD=os'.
MlAa2 UPranPhaer00lobi6

3.4TtuhsmittedLight Operution
- Da*Jield
Da*neldobseraionmode
issinil.rlo bdshtfieldexceflhalonlyindieclo.obtiquelislt lhar
h bdf in
fi_ontnsedscsoflheficldorliew n iluninatinSthespdncn. TheEsullisa
specinn
wi$ bn8!t cd86againslada* btclgoundor"da*teld .
Darknehisuscdtoobsery€
verysmalllivirs oqlalicor8dism, diabms,smaUins{Ls.bone,
fibe6,han,unsuined
batria, y€ast, cells,
protozoa
andorhetbiological
sp*idms.
MetiTcchnosMT6000S.nes
Modcl!htlc oplional50Xsd l00Xoilimne6ionPld
objecdws witn insdd oprional
ddncb ond6$r wilh NA of |.25ir adovetailmount
SettingUpD.rkfield
ObservrtionMode
Th. fo[o*Im iclructloB .s!ud. vonhrv. . or...rcd tud. with cov.r .lip.
L lnsrll thedarkieldobjetivesinlo$en$epicce.
2 Rdolc iheshndardcondns byl@snir8 iljerhmbscw holdingth. cordo$r sembly
andsinptyslidi.gih.
condmsertowadslouuclaYou
nigil ock dow.lh. @ndens.r ronalc
$eacce$easid.
slidcintncddtfieldoilio6.6io. condenserin
pl.cc
and
snugrhe
3.Tocheckrow if thcdailield condmsr iscolcr€d, l@k intolhecy.lubes
vith lheEeuld
l0x objeriveinposition.Ialheiis cannorecliperbedadr.ld
sropcoftcd, adjlstthe
conde.serlhurbscawsunil inc{opcmbecentcrcd
poperly.
4.Plrc. r dmportwoorimdio. oil onlhecdLr oflh. lopollhc condsr andnove !p lhe
condenerEckrorhe Eq redhekhr,i.e.lhe imncFion oil is inconbcrwithlhehorloh ofth.
5 Pl&e adrcpofoil onlothe@vcrslipahvc you.specincnand
slovly dd cs&lly Ed€ dr
50xdrr*lieldoilobjeriveorrhel00Xdn*6cldoilohj.clivcintodcdmpoaoillh nonbp
ofln€covcrslip.Adj$l $e locusof
your
tecimen.
Thenicro$oDc isnowir ddLfi.ld obsnalion oode.