Olympus Fluoview FV1000 Installation guide

Confocal Laser Scanning
Biological Microscope
FV1000
FLUOVIEW
FLUOVIEW—Always Evolving

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FLUOVIEW–—From
Olympus is Open
FLUOVIEW—More Advanced than Ever
The Olympus FLUOVIEW FV1000 confocal laser scanning microscope delivers
efficient and reliable performance together with the high resolution required for
multi-dimensional observation of cell and tissue morphology, and precise
molecular localization. The FV1000 incorporates the industry’s first dedicated
laser light stimulation scanner to achieve simultaneous targeted laser stimulation
and imaging for real-time visualization of rapid cell responses. The FV1000 also
measures diffusion coefficients of intracellular molecules, quantifying molecular
kinetics. Quite simply, the FLUOVIEW FV1000 represents a new plateau, bringing
“imaging to analysis.”
Olympus continues to drive forward the development of FLUOVIEW
microscopes, using input from researchers to meet their evolving demands and
bringing “imaging to analysis.”
Quality Performance with Innovative Design
FV10i

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Imaging to Analysis
ing up New Worlds
From Imaging to Analysis
FV1000
Advanced Deeper Imaging with High Resolution
FV1000MPE

Advanced FLUOVIEW Systems Enhance the Power of
Your Research
Superb Optical Systems Set the Standard
for Accuracy and Sensitivity.
Two types of detectors deliver enhanced accuracy and sensitivity,
and are paired with a new objective with low chromatic aberration,
to deliver even better precision for colocalization analysis.
These optical advances boost the overall system capabilities and raise
performance to a new level.
Imaging, Stimulation and Measurement—
Advanced Analytical Methods for Quantification.
Now equipped to measure the diffusion coefficients of intracellular
molecules, for quantification of the dynamic interactions of molecules
inside live cell.
FLUOVIEW opens up new worlds of measurement.
Evolving Systems Meet the Demands of Your Application.
Upgradeable system with optional hardware and software to meet
the demands of your research.
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Laser combiner/Fiber
Diode Laser
Greater stability, longer service life and
lower operating cost are achieved using
diode lasers.
Laser Feedback Control
Scanner unit is equipped with laser power
monitor for feedback control enhancing
stable laser output.
Laser Compatibility
Diode laser :
405 nm, 440 nm, 473 nm, 559 nm, 635 nm
Gas laser :
Multi-line Ar laser (458 nm, 488 nm, 515 nm)
HeNe(G) laser (543 nm)
Broadband Fiber
Broadband fiber connection for 405–635 nm
lasers, to achieve an ideal point light source
with minimal color shift and position shift
between images.
LD405
LD473
LD559
LD635
AOTF
Grating
Barrier filter
Confocal pinhole
Grating
AOTF
Laser combiner
Main scanner
Broadband fiber *
Broadband fiber
Excellent Precision, Sensitivity and Stability.
FLUOVIEW Enables Precise, Bright Imaging with Minimum Phototox
Two versions available.
•Single fiber-type combiner is used for
main scanner FV1000 with up to six lasers,
ranging from 405 to 635 nm.
•Dual fiber-type combiner is used for laser
light stimulation with main and SIM
scanner FV1000.
Laser Combiner
Scanners/Detection
High Sensitivity Detection System
High-sensitivity and high S/N ratio optical
performance is achieved through the
integration of a pupil projection lens, use
of a high sensitivity photomultiplier tube
and an analog processing circuit with
minimal noise. Enables high S/N ratio
image acquisition with minimal laser power
to reduce phototoxicity.
Up to Four PMT Channels
Three integrated confocal PMT detectors,
and optional module with fourth confocal
PMT expandable up to four PMT channels.
Two Versions of Light Detection System
• Spectral detection for high-precision
spectroscopy with 2 nm resolution.
• Filter detection equipped with high
quality filter wheels.
Spectral Scanning Unit Filter Scanning Unit
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Technology / Hardware
IX81
PMT
PMT
PMT
Galvanometer
scanning mirrors
Galvanometer
scanning mirrors
UIS2 objectives
Specimen
Pupil
projection
lens
SIM Scanner *
Microscope
icity.
Optical System
Motorized Microscopes
Compatible with Olympus IX81 inverted
microscope, BX61WI focusing nosepiece
and fixed-stage upright microscope, and
BX61 upright microscope.
Samples and Specimens
Supports a Wide Range of Samples and
Specimens
Tissue culture dishes, slide chambers,
microplates and glass slides can be used
with live cells and fixed specimens.
BX61
UIS2 Objectives
Olympus UIS2 objectives offer world-
leading, infinity-corrected optics that
deliver unsurpassed optical performance
over a wide range of wavelengths.
High S/N Ratio Objectives with
Suppressed Autofluorescence
Olympus offers a line of high numerical
aperture objectives with improved
fluorescence S/N ratio, including
objectives with exceptional correction for
chromatic aberration, oil- and water-
immersion objectives, and total internal
reflection fluorescence (TIRF) objectives.
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* Option

Spectral Based Detection
Flexibility and High Sensitivity
Spectral detection using gratings
for 2 nm wavelength resolution
and image acquisition matched to
fluorescence wavelength peaks.
User adjustable bandwidth of
emission spectrum for acquiring
bright images with minimal cross-
talk.
Precise Spectral Imaging
The spectral detection unit uses a grating method that offers
linear dispersion compared with prism dispersion. The unit
provides 2 nm wavelength resolution to high-sensitivity
photomultiplier tube detectors. Fluorescence separation can be
achieved through unmixing, even when cross-talk is generated
by multiple fluorescent dyes with similar peaks.
496 500 504 508 512 516 520 524
Wavelength
Intensity
528 532 536 540 544 548 552
400
600
800
1,000
1,200
1,400
1,600
1,800
2,000
2,200
2,400
2,600
EGFP (dendrite) — EYFP (synapse)
XYλ
Wavelength detection range: 495 nm–561 nm in
2 nm steps
Excitation wavelength: 488 nm
Courtesy of: Dr. Shigeo Okabe
Department of Anatomy and Cell Biology,
Tokyo Medical and Dental University
EGFP–EYFP Fluorescence Separation
EYFP
EGFP
480
Conventional mirror unit High-performance mirror unit
500 520 540 560 580
Wavelength (nm)
600 620 640 660 680 700
0
20
40
Transmittance (%)
60
80
100
DM488/543/633 Comparison
Two Versions of Light Detection System that Set New Standards
for Optical Performance.
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Filter Based Detection
Enhanced Sensitivity
Three-channel scan unit with detection system featuring hard
coated filter base. High-transmittance and high S/N ratio optical
performance is achieved through integration of a pupil projection
lens within the optics, the use of a high sensitivity photomultiplier
and an analog processing circuit with minimal noise.
High-Performance Filters Deliver Outstanding Separation
Special coatings deliver exceptionally sharp transitions to a
degree never achieved before, for acquisition of brighter
fluorescence images.

Technology / Hardware
SIM (Simultaneous) Scanner Unit
Combines the main scanner with a dedicated laser light
stimulation scanner for investigating the trafficking of fluorescent-
labeled molecules and marking of specific live cells.
Simultaneous Laser Light Stimulation and Imaging
Performs simultaneous laser light stimulation and imaging to
acquire images of immediate cell responses to
stimulation in photobleaching experiments.
Modifiable Stimulation Area During Imaging
The stimulation area can be moved to a different position on the
cell during imaging, providing a powerful tool for photoactivation
and photoconversion experiments.
Wide Choice of Bleaching Modes
Various scan modes can be used for both the observation area
and stimulation area. Enables free-form bleaching of designated
points, lines, free-lines, rectangles and circles.
SIM Scanner Unit for Simultaneous Laser Light Stimulation and
Imaging.
Branching of laser in
laser combiner.
Lasers are used for both imaging
and laser light stimulation.
LD405
LD635
AOTF
AOTF
LD473
LD559
Multi-Purpose Laser Combiner
All lasers can be used for both Imaging and laser light stimulation.
LD405 / LD635 / AOTF / AOTF / LD473 / LD559
Laser Sharing with Main Scanner
Dual fiber laser combiner provides laser sharing between the SIM
scanner and main scanner, eliminating the need to add a
separate laser for stimulation.
Unique "Tornado" Scanning for Efficient Bleaching
Conventional raster scanning does not always complete
photobleaching quickly. Tornado scanning greatly improves
bleaching efficiency by significantly reducing unnecessary
scanning.
*Tornado scanning only available for SIM scanner.
Tornado scanning
Superfluous scanning areas.
ROI (region of interest)
scanning
ROI (region of interest) scanning.
Tornado scanning.
Cell membrane stained with DIO, and subjected to both
conventional ROI and tornado scanning.
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Low Chromatic Aberration Objective
Best Reliability for Colocalization Analysis
A new high NA oil-immersion objective minimizes chromatic
aberration in the 405–650 nm region for enhanced imaging
performance and image resolution at 405 nm. Delivers a high
degree of correction for both lateral and axial chromatic
aberration, for acquisition of 2D and 3D images with excellent
and reliable accuracy, and improved colocalization analysis. The
objective also compensates for chromatic aberration in the near
infrared up to 850 nm.
New Objective with Low Chromatic Aberration Delivers
World-Leading Imaging Performance.
NEW
NEW
Low Chromatic PLAPON60xOSC
Aberration Objective Magnification: 60x
NA: 1.4 (oil immersion)
W.D.: 0.12 mm
Chromatic aberration compensation range: 405–650 nm
Optical data provided for each objective.
3D image
Tubulin in Ptk2 cells labeled with
two colors (405 nm, 635 nm) and
compared.
Improved Flatness and Resolution at 405 nm
Better flatness reduces the number of images for tiling.
450400
-0.5
0.0
Focal plane (µm)
0.5
Lateral Chromatic Aberration
Wavelen
g
th (nm)
500 550 600 650
PLAPON 60xOSC
UPLSAPO60xO
UPLSAPO60xO
Chromatic Aberration Comparison for PLAPON 60xOSC and
UPLSAPO 60xO
Performance Comparison of PLAPON 60xOSC and UPLSAPO 60xO
PLAPON60xOSC
Axial chromatic
aberration (Z direction)
Compared for PSF fluorescent
beads (405 nm, 633 nm).
Lateral chromatic
aberration (X-Y direction)
Compared for PSF fluorescent
beads (405 nm, 488 nm, 633
nm).
*Chromatic aberration values are design values and are not guaranteed values.
Lateral and Axial Chromatic Aberration
Small Degree of
Chromatic Aberration
Large Degree of
Chromatic Aberration
Axial chromatic
aberration
(Z direction). Lateral chromatic
aberration (X-Y
direction at FN6).
Approx.
0.5 µm
Approx.
0 µm
Approx. 0.1 µm Approx. 0.2 µm
Objective
PLAPON60xOSC UPLSAPO60xO
Flatness Comparison Image at 1x Zoom
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