Memmert ICOmed Series User manual

www.memmert.com |www.atmosafe .net
100% ATMOSAFE. MADE IN GERMANY.
ICOmed
with IVF module
ADDITION TO
OPERATING MANUAL
OPERATING MANUAL
OPERATING MANUAL
CO
2
2
2
INCUBATOR ICOMED WITH IVF MODULE
ICO50MED
ICO105MED

Manufacturer and
customer service
MEMMERT GmbH + Co. KG
Willi-Memmert Strasse 90-96
91186 Büchenbach
Germany
Phone: [phone]
Fax: [phone]
E-mail: [email protected]
Internet: www.memmert.com
Customer service:
Service hotline: [phone]
Service fax: [phone]
E-mail: [email protected]
When calling for service enquiries, please state the appliance number given on the nameplate
(see page 10).
Shipping address for repairs:
Shipping address for repairs:
Memmert GmbH + Co. KG
Customer service
Willi-Memmert Str. 90-96
91186 Büchenbach
Germany
Please contact our customer service department before sending appliances for repair or before
returning equipment, or the shipment may be refused.
© 2018 MEMMERT GmbH + Co. KG
D39322 | Date 03/2018
Subject to change without notice

D39322 | Date 03/2018 3
About this manual
About this manual
Purpose and target audience
These instructions are an addition to the operating manual for the ICOmed CO
2
incubator
when using the IVF module. It is intended for use by trained personnel of the owner, who
have the task of operating and/or maintaining the respective appliance.
If you are asked to work on the appliance, read these instructions and the operating manual
for the ICOmed CO
2
incubator carefully before starting. Familiarise yourself with the safety
regulations. Only perform work that is described in this manual. If there is something you do
not understand, or certain information is missing, ask your manager or contact the manufac-
turer. Do not do anything without authorisation.
Versions
Due to individual configurations and sizes, illustrations in this manual may be slightly different
to the actual appearance. Function and operation are identical.
Other documents to be observed:
►
the operating manual for
the ICOmed CO
2
incubator
►
For operation of the appliance with MEMMERT AtmoCONTROL, observe the separate
software manual
►
For service and repair work, observe the separate service manual
Storage and resale
This operating manual belongs with the appliance and should always be stored where per-
sons working on the appliance have access to it. It is the owner's responsibility to ensure that
persons who are working on or are going to work on the appliance know where to find the
operating manual. We recommend that it is always stored in a protected location close to the
appliance. Make sure that the operating manual is not damaged by heat or humidity. If the
appliance is resold or transported and then set up again at a different location, the operating
manual must remain with it.

4 D39322 | Date 03/2018
Contents
Contents
1. Safety regulations 5
1.1 Terms and signs used
...........................................................................................................
5
1.1.1 Terms used
........................................................................................................................
5
1.1.2 Signs used
.........................................................................................................................
5
1.2 Product safety and dangers
................................................................................................
6
1.3 Intended use
........................................................................................................................
1.3 Intended use........................................................................................................................1.3 Intended use
7
1.4 Requirements of the operating personnel
..........................................................................
7
1.5 Responsibility of the operator
.............................................................................................
1.5 Responsibility of the operator .............................................................................................1.5 Responsibility of the operator
8
1.6 Changes and alterations
......................................................................................................
8
1.7 Behaviour in case of malfunctions and irregularities
..........................................................
8
1.8 What to do in case of accidents
..........................................................................................
8
1.9 Switching off the appliance in an emergency
....................................................................
1.9 Switching off the appliance in an emergency ....................................................................1.9 Switching off the appliance in an emergency
9
2. Design and description 10
2.1 Design
................................................................................................................................
10
2.2 Description
.........................................................................................................................
10
2.3 Standard package
..............................................................................................................
2.3 Standard package..............................................................................................................2.3 Standard package
11
3. IVF-Module installation 12
3.1 Additional package for self-installation of the IVF module
..............................................
3.1 Additional package for self-installation of the IVF module ..............................................3.1 Additional package for self-installation of the IVF module
12
3.2 Assembly
............................................................................................................................
3.2 Assembly............................................................................................................................3.2 Assembly
12
3.2.1 Cleaning
..........................................................................................................................
12
3.2.2 Checking the temperature sensor
...................................................................................
3.2.2 Checking the temperature sensor...................................................................................3.2.2 Checking the temperature sensor
13
3.2.3 Fitting the installation frame
..........................................................................................
3.2.3 Fitting the installation frame ..........................................................................................3.2.3 Fitting the installation frame
13
3.2.4 Insert IVF module
............................................................................................................
3.2.4 Insert IVF module ............................................................................................................3.2.4 Insert IVF module
15
3.2.5 Removing the drawers
....................................................................................................
16
3.2.6 Remove IVF module
........................................................................................................
3.2.6 Remove IVF module ........................................................................................................3.2.6 Remove IVF module
17
4. Putting into operation 18
4.1 Connecting the appliance
.................................................................................................
4.1 Connecting the appliance .................................................................................................4.1 Connecting the appliance
18
4.2 Switching on the appliance
...............................................................................................
4.2 Switching on the appliance...............................................................................................4.2 Switching on the appliance
18
4.3 Sterilising the appliance
....................................................................................................
4.3 Sterilising the appliance ....................................................................................................4.3 Sterilising the appliance
18
4.3.1 Preparation
......................................................................................................................
18
5. Operation 20
5.1 Preparation
........................................................................................................................
20
5.2 Start the incubation process
.............................................................................................
20
5.3 After the incubation process
.............................................................................................
20
6. Cleaning 21

D39322 | Date 03/2018 5
Safety regulations
1.
Safety regulations
1.1
Terms and signs used
In this manual and on the appliance itself, certain common terms and signs are used to warn
you of possible dangers or to give you hints that are important in avoiding injury or damage.
Observe and follow these notes and regulations to avoid accidents and damage. These terms
and signs are explained below.
1.1.1
Terms used
WARNING
Warns of a dangerous situation that might lead to death or
serious injuries
CAUTION
Warns of a dangerous situation that might lead to moderate
or minor injuries
NOTICE
Warns of material damage
1.1.2
Signs used
Danger of elec-
trocution
Fire hazard
Gases / vapours
Danger of frost-
bite/
cold burns
Warning of
gas
bottles
Danger of burns
Disconnect the
mains plug
Wear gloves
Wear safety
boots
Observe informa-
tion in separate
manual
Information on
first aid
First aid:
Rinse eyes out
Important or
useful
additional infor-
mation

6 D39322 | Date 03/2018
Safety regulations
1.2
Product safety and
dangers
The appliances described in this manual are technically sophisticated, manufactured using
high-quality materials and subject to many hours of testing in the factory. They reflect the
state of the art and comply with recognised technical safety regulations. However, there are
still risks involved, even when the appliances are used as intended. These are described below.
WARNING
After removing covers, live parts may be exposed. Touching these
can lead to an electrical shock. Disconnect the mains plug before
removing any covers. Work on the electrical system must only be
performed by qualifi ed electricians.
WARNING
When loading the appliance with an unsuitable load, poisonous
or explosive vapours or gases may be produced. This could cause
the appliance to explode, and people could be severely injured or
poisoned. The appliance may only be loaded with materials / test
objects that do not emit any poisonous or explosive vapours when
heated up.
WARNING
Leaving the door open during operation can cause the appliance to
overheat or pose a fi re hazard. Do not leave the door open during
operation.
WARNING
Depending on operation, the surfaces in the interior of the
appliance and the chamber load may still be very hot after
the appliance is switched off. Touching these surfaces can
cause burns. Wear heat-resistant protective gloves or wait
until the appliance cools down before touching.
WARNING
Gas bottles may burst or explode at high temperatures. Keep the
gas bottles away from open fl ames.
Store gas bottles below 50 °C
and ensure that the location is always well ventilated. Prevent water
from penetrating as well as backfl ow into the gas bottles. It is es-
sential that you read the safety notes and instructions of the gas
supplier.
WARNING
Condensation in the electrical components may cause short circuits.
After transporting or storing the device under humid conditions, re-
move it from its packaging and let it ventilate for at least 24 hours in
normal environmental conditions. Do not connect the device to the
mains power during this time.

D39322 | Date 03/2018 7
Safety regulations
CAUTION
Danger of suffocation. CO
2
and N
2
in high concentrations can have
a suffocating effect. In normal mode, the appliance emits small
2
a suffocating effect. In normal mode, the appliance emits small
2
2
a suffocating effect. In normal mode, the appliance emits small
2
amounts of CO
2
and N
2
to its surroundings. You should therefore en-
sure that the room in which it is installed is properly ventilated. You
2
sure that the room in which it is installed is properly ventilated. You
2
2
sure that the room in which it is installed is properly ventilated. You
2
will need to extract 250 m³ of air per hour as a minimum. Always
close the stop valve or pressure reducer on the
gas bottles if the ap-
pliance is not in operation.
CAUTION
High concentrations of CO
2
can cause
cold burns or frostbite. Avoid
contact with CO
2
gas to the eyes and skin.
2
gas to the eyes and skin.
2
CO
2
and N
2
are not dangerous substances in terms of the German Hazardous Substances
Ordinance (GefStoffV). You should nevertheless familiarise yourself with the applicable safety
regulations prior to handling such
gas bottles.
1.3
Intended use
The CO
2
incubator ICOmed with IVF module is used to generate and maintain constant ambi-
ent conditions for the in-vitro fertilisation (IVF) application field, especially for the incubation
of oocytes, spermatozoa and zygotes in special culture dishes for IVF application as well as for
gene expression and the biosynthesis of RNA and proteins.
1.4
Requirements of the
operating personnel
The incubator may only be operated and maintained by persons who are of legal age, and
who have received instructions for the incubator. Personnel who are to be trained, instructed
or who are undergoing general training may only be active on the incubator under the con-
tinuous supervision of an experienced person.
The incubator may only be transported by persons (fork-lift truck, manual pallet jack), who are
trained for this work and who know the corresponding safety regulations.
Repairs may only be performed by qualified electricians. The regulations in the separate service
manual must be observed.

8 D39322 | Date 03/2018
Safety regulations
1.5
Responsibility of the operator
The operator of the incubator
►
is responsible for the flawless condition of the incubator and for the incubator being oper-
ated in accordance with its intended use;
►
is responsible for ensuring that persons who are to operate or service the incubator are
qualified to do this, have received instructions about the incubator and are familiar with
this operating manual;
►
must know about the applicable guidelines, requirements and operational safety regula-
tions, and train staff accordingly;
►
is responsible for ensuring that unauthorised persons have no access to the incubator;
►
is responsible for ensuring that the maintenance plan is adhered to and that maintenance
and repair work is properly carried out;
►
ensures, for example through corresponding instructions and inspections, that the incuba-
tor and its surroundings are kept clean and tidy;
►
is responsible for ensuring that personal protective clothing is worn by operating person-
nel, e.g. work clothes, safety shoes and protective gloves.
1.6
Changes and alterations
No independent conversions or alterations may be made to the incubator. No parts may be
added or inserted which have not been approved by the manufacturer.
Unauthorised changes or alterations result in the EC declaration of conformity losing its valid-
ity, and the incubator may no longer be operated.
The manufacturer is not liable for any damage, danger or injuries that result from unauthor-
ised changes or alterations, or from non-compliance with the provisions in this manual.
1.7
Behaviour in
case of malfunctions and irregularities
The incubator may only be used when in a flawless condition. If you as the operator notice
irregularities, malfunctions or damage, put the incubator out of service immediately (see
Chapter 1.9) and inform your superiors.
1.8
What to do in case of
accidents
1.
Keep calm. Act with determination and consideration. Pay attention to your
own safety.
2.
Switch off the incubator and close the valves on the gas bottle.
3.
Call a doctor.
4.
Start first aid measures. If available: Call a trained first aid helper.
In case of contact with CO
2
2
to the eyes and skin:
to the eyes and skin:
Rinse eyes out with water for at least 15 minutes. In case of cold burns, rinse with
water for at least 15 minutes. Cover over in a sterile way. Call a doctor.

D39322 | Date 03/2018 9
Safety regulations
After inhaling CO
After inhaling CO
2
2
:
High concentrations can cause suffocation. Symptoms may include a loss of mobility and
unconsciousness. The victim is not aware of suffocating.
Low concentrations of CO
2
can cause accelerated breathing and headaches.
Anyone affected should breathe fresh air, using a breathing device independent of recircu-
lating air. Keep the person warm and calm. Call a doctor. In case of respiratory arrest, use
artificial respiration.
If gas is escaping:
If gas is escaping:
Leave the room immediately, warn others and ventilate the room. If you re-enter the room,
use a breathing device independent of recirculating air if it has not been established that the
atmosphere is harmless.
1.9
Switching off the appliance in an
emergency
Press the main switch on the ControlCOCKPIT
(Fig. 1) and disconnect the power plug. This
disconnects the appliance from the power
supply at all poles.
Hauptschalter
>
Zu der Typenbezeichnung gibt es momentan drei Entwurfsrichtungen,
ich kann Ihnen noch nicht sagen, ob Memmert hierzu schon eine Entscheidung
getroen hat.
Die hier gezeigt Variante, ist die von uns empfohlene Richtung.
UF 110PLUS
ON
344.4
444 4.
TEMP
Set
°C
°C
FLAP
40%
TIMER
hd 1002
End Sept.29 22 24
FAN
%0
%
100
ALARM
max
444.4°C
auto +/-0.0K
min
444.4°C
O
O
ON
N
N
N
O
O
N
O
Manu
44.Sept
Fig. 1
Switch off the appliance by pressing the
main switch

10
D39322 | Date 03/2018
Design and description
2.
Design and description
2.1
Design
1
2
4
3
5
6
7
Fig. 2
Design
1
ControlCOCKPIT with capacitive function keys and LCD displays (for description and opera-
Fig. 2
ControlCOCKPIT with capacitive function keys and LCD displays (for description and opera-
Fig. 2
Design
ControlCOCKPIT with capacitive function keys and LCD displays (for description and opera-
Design
tion see ICOmed operating instructions)
ControlCOCKPIT with capacitive function keys and LCD displays (for description and opera-
tion see ICOmed operating instructions)
ControlCOCKPIT with capacitive function keys and LCD displays (for description and opera-
2
Main switch
tion see ICOmed operating instructions)
Main switch
tion see ICOmed operating instructions)
3
Outlet
4
IVF module
5
Markable drawers
6
Unlocking handle
7
Inner glass door
Unlocking handle
Inner glass door
Unlocking handle
2.2
Description
The ICOmed CO
2
incubator with IVF module is used to generate and maintain constant
ambient conditions for the in-vitro fertilisation (IVF) application field. For this purpose, it is
equipped with a slide-in unit - the IVF module - which in turn contains eight (ICOmed 105)
and six (ICOmed 50) drawers, into which Petri dishes and 4-well plates as well as tube holders
can be inserted on the included inserts.
The drawers in the IVF module can be pulled out individually after opening the door to insert
or remove inserts and preparations.