Memmert INCO 108 User manual

OPERATING MANUAL
CO2incubators
INCO 108 INCO 108 med
INCO 153 INCO 153 med
INCO 246 INCO 246 med

M
anufactur
e
r and
c
ust
o
m
e
r s
e
rv
i
c
e
M
EMMERT Gm
b
H + C
o
. KG
P
ostfach 17 2
0
D
-911
0
7 Sc
h
wa
b
ac
h
Ä
uß
e
r
e
Ritt
e
rsbach
e
rstr. 3
8
D
-9112
6
Sc
h
wa
b
ac
h
German
y
F
on: [phone] / 925-0
F
ax: [phone] / 14585
E
-Mai
l
: sa
l
es
@
memmert.co
m
I
nt
e
rn
e
t: www.m
e
mm
e
rt.c
o
m
C
ust
o
m
e
r s
e
rv
i
c
e:
F
on: [phone]/925-128
and: [phone]/925-126
E
-Mai
l
: service
@
memmert.co
m
F
or service enquiries, please always specify the appliance number on the nameplate (see pa
g
e
15).
© 2
0
14 Memmert Gm
b
H + Co. KG
Ed
ition 11/2014
Subject to modifications

3
A
bou
t
t
hi
s
manual
A
bo
ut t
h
is manua
l
P
urpose an
d
tar
g
et
g
rou
p
T
his manual describes the setu
p
, function, o
p
eration and maintenance of CO
2
incu
b
at
o
rs
o
f the types INCO and INCOmed with a chamber volume of 108, 153 and 246 litres. In this
manual, the term INCO is used for INCO as well as INCOmed for the purpose of simplification.
D
istinctions are exp
l
icit
l
y pointe
d
out.
T
his manual is intended for use by the trained staff of the operator in char
g
e of operatin
g
and/or maintainin
g
the incubator. If you are asked to work on the incubator, you should read
this manual carefully before startin
g
work on the unit. Familiarise yourself with the safety
re
g
ulations. Only perform the work that is described in this manual. If there is somethin
g
you
d
on't understand, or certain information is missin
g
, ask your superior or
g
et in touch with the
manufacturer. Do not do anythin
g
without authorisation
.
Co
nt
e
nts
T
he INCO incubator is available with different fittin
g
s: as a basic model, and with six different
additional modules. The technical fittin
g
s and functional ran
g
e of the basic model and the
individual additional modules are described from pa
g
e 11.
I
f specific equipment features or functions are available only with one of the additional mod-
ul
es, t
h
is is pointe
d
out in t
h
e re
l
evant sections in t
h
is manua
l
.
D
ue to individual fittin
g
s, depictions in this manual may be different from the actual appear-
an
c
e.
O
t
h
er
d
ocuments t
h
at you must rea
d
:
Ź
f
or service and repair work (see pa
g
e
59
) – a separate service manua
l
S
tora
g
e an
d
Forwar
d
in
g
Th
is instruction manua
l
b
e
l
on
g
s wit
h
t
h
e incu
b
ator an
d
s
h
ou
ld
a
l
ways
b
e store
d
so t
h
at t
h
ose
who work on the incubator have access to it. It is the responsibility of the operator to ensure
that persons who work on or who will work on the incubator are informed as to the wherea-
bouts of this instruction manual. We recommend that it is always stored in a protected loca-
tion c
l
ose to t
h
e incu
b
ator. Ma
k
e sure t
h
at t
h
e instruction manua
l
is not
d
ama
g
e
d
b
y
h
eat or
d
amp. If the incubator is sold on or transported and then set up a
g
ain at a different location,
t
h
is instruction manua
l
must
g
o wit
h
it
.

4
C
ontent
Co
nt
e
nt
1
. Safety re
g
ulations 6
1.1 T
e
rms an
d
ic
o
ns us
ed
..........................................................................................................
6
1.2 Product safety and dan
g
ers ................................................................................................
7
1.3 Requirements of the operatin
g
personnel..........................................................................
8
1.4 Responsibility of the owner
.................................................................................................
r
8
1.5 C
h
an
g
es an
d
conversion
s
....................................................................................................
8
1.6 Behaviour in case of malfunctions and irre
g
ularitie
s
..........................................................
8
1.7 What t
o
d
o
in cas
e
o
f accid
e
nts..........................................................................................
9
1.8 Switching off incubator in an emergency
...........................................................................
y
9
2. Desi
g
n an
d
Function 1
0
2.1 Des
ig
n................................................................................................................................1
0
2.2 Funct
io
n.............................................................................................................................11
2.3 Optiona
l
extras..................................................................................................................11
2.4 Mat
e
ria
l
.
.............................................................................................................................1
3
2.5 E
l
ectrica
l
equipment
..........................................................................................................
1
3
2.
6
C
o
nn
e
ct
io
ns.......................................................................................................................1
3
2.7 Intended use
......................................................................................................................
14
2.8 EC Declaration of Conformit
y
.
...........................................................................................15
2.9 Desi
g
nation (nameplate
)
...................................................................................................15
2.1
0
Tec
h
nica
l
d
ata....................................................................................................................1
6
2.11 Am
b
i
e
nt c
o
n
d
iti
o
ns...........................................................................................................1
8
2.12 Acc
e
ss
o
ri
e
s inc
l
u
ded
..........................................................................................................1
8
3
. De
l
ivery, Transport an
d
Settin
g
Up 1
9
3
.1 Safety re
g
ulations..............................................................................................................1
9
3
.
2
Transport............................................................................................................................1
9
3.3 Delivery
..............................................................................................................................
y
1
9
3
.4
S
etup
.
.................................................................................................................................2
0
4
. Putt
i
n
g
i
nto Operat
i
on 2
2
4
.1 C
he
c
k
s
................................................................................................................................
22
4
.2 Connect
i
n
g
........................................................................................................................
22
4
.3 Oxy
g
en ca
l
i
b
ratio
n
.............................................................................................................
2
4
5. Operation an
d
contro
l
2
5
5.1 Operatin
g
personne
l
.
.........................................................................................................
2
5
5.2 Openin
g
t
h
e
d
oor ............................................................................................................
2
5
5.3 Loading the incubator
.......................................................................................................
r
2
5
5.4 Insertin
g
water tray(s
)
.......................................................................................................
26
5.5 Connect gas supply
...........................................................................................................
y
26
5.6 Switch on appliance
..........................................................................................................
e
26
5.7 Bas
i
c operat
i
on..................................................................................................................
2
7
5.8 Sett
i
n
g
parameters............................................................................................................
2
7
5.9 Operatin
g
mo
d
es...............................................................................................................2
8
5.10 Setting the operating mode
..............................................................................................2
8
5.11 Dur
i
n
g
operat
i
on...............................................................................................................
3
4
5.12 En
d
in
g
operation...............................................................................................................
3
7
6. Warnin
g
messa
g
es and malfunctions 3
8
6.1 Warn
i
n
g
messa
g
e
s
............................................................................................................
38
6.2 System/app
l
iance errors ....................................................................................................
39
6.3 Power failure
......................................................................................................................
e
42

5
C
ontent
7. Advanc
e
d functi
o
ns 4
3
7.1 Pr
i
nt
e
r
...............................................................................................................................
43
7.2 Basic appliance settin
g
s (Setup)
........................................................................................
43
7.3 Temperature mon
i
tor
i
n
g
..................................................................................................
4
5
7.4 Steri
l
isation c
h
ipcar
d
.........................................................................................................
49
7.5 User ID card (optionally available as an accessory
)
...........................................................
50
7.
6
Ca
l
i
b
rati
o
n.........................................................................................................................
50
7.7 C
o
mmunicati
o
n int
e
rfac
es
................................................................................................
5
5
7.8 Log memory
......................................................................................................................
y
58
8
. Maintenance an
d
Servicin
g
5
9
8
.1 C
l
eanin
g
.............................................................................................................................
59
8
.2 Re
g
ular maintenanc
e
.........................................................................................................
59
8.3 Adjusting door
...................................................................................................................
r
60
8.4 Repairs and Service
............................................................................................................
e
60
9. Stora
g
e an
d
Disposa
l
6
1
9.1 Storage
..............................................................................................................................
e
6
1
9.2 Disposa
l
.............................................................................................................................
6
1
I
n
de
x
6
2

6
S
afety regulations
1.
Safety re
g
ulations
1.1
Te
rms an
d
ic
o
ns us
ed
I
n this manual, certain common terms and icons are used to warn you of dan
g
ers or to
g
ive
you notes that are important in avoidin
g
injury or dama
g
e. Observe and follow these notes
an
d
re
g
u
l
ations to avoi
d
acci
d
ents an
d
d
ama
g
e. T
h
ese terms an
d
icons are exp
l
aine
d
b
e
l
ow.
1.1.1
Te
rms us
ed
"Warn
i
ng
"
is always used whenever you or somebody else could be injured if you do
n
ot observe the accompanyin
g
safety re
g
ulation
.
"
C
aution
"
is used for information that is important for avoidin
g
dama
g
e
.
1.1.2
I
c
o
ns us
ed
Prohibited sign (forbidding an action)
Do
n
o
t ti
l
t
app
l
ianc
e
D
o not lift appliance
Warning icons (warning of a danger)
D
an
g
er of
ele
ctrica
l
s
ho
c
k
E
xp
l
osive
atmosp
h
er
e
D
an
g
er of frost
-
b
ite/co
ld
b
urns
Warnin
g
of
g
as
b
o
ttl
e
sheat/hot surface
s
Ga
s
Regulation signs (stipulate an action)
D
isc
o
nn
e
ct t
he
mains p
l
u
g
Wear
gl
oves Wear safety
shoe
s
Ob
serve
inf
o
rmati
o
n in
s
eparate manua
l
T
w
o
o
r m
o
r
e
persons require
d
Other icons
I
nf
o
rmati
o
n
o
n
f
irst ai
d
F
irst Ai
d
:
Ri
nse eyes out
I
mportant or
u
s
e
ful additi
o
n-
al inf
o
rmati
on

7
S
afety regulations
1.2
P
roduct safety and
d
an
g
er
s
I
ncubators of the INCO type are technically well-developed, manufactured usin
g
hi
g
h-quality
materials and tested for many hours in the factory. They contain the latest technolo
g
y and
c
omply with reco
g
nised technical safety re
g
ulations. But there are still dan
g
ers involved, even
w
h
en t
h
e app
l
iance is use
d
as inten
d
e
d
. T
h
ese
d
an
g
ers are
d
escri
b
e
d
b
e
l
ow.
W
arning
!
A
fter removing covers, live parts may be exposed. You may receive
an electric shock if you touch these parts. Disconnect the mains plug
b
efore removing any covers. Any work inside the unit may only be
p
erformed by qualified electricians.
W
arning
!
W
hen loading the chamber with an unsuitable load, poisonous or
explosive vapours or gases may be produced. This could cause the
chamber to explode, and people could be badly injured or poisoned.
The chamber may only be loaded with materials/test objects which
d
o not form any poisonous or explosive vapours when heated up
(
see also Chapter 2.7 In
te
nd
e
d u
se
on page 14).
W
arn
i
ng
!
D
anger of suffocation. In high concentrations, CO
2
an
d
N
2
c
an
h
av
e
a suffocating effect. In normal operation, the incubator gives off
s
mall amounts of
CO
2
and – if equipped with the O
2
mo
d
u
le
– N
2
t
o i
ts
e
nvironm
e
n
t
. You
s
hould
t
h
e
r
e
for
e
e
n
s
ur
e
t
ha
t
t
h
e
room in
which it is installed is properly ventilated. If a gas bottle is not con-
n
ecte
d
, or
i
s empty, a
l
ways c
l
ose t
h
e stop va
l
ve or pressure re
d
ucer
on
the
b
o
ttle
.
W
arn
i
ng
!
H
igh concentrations of CO
2
c
an
c
au
se
c
old burn
s
or fro
st
bi
te
. Avoid
contact w
i
t
h
CO
2
gas to t
h
e eyes an
d
s
ki
n.
W
arn
i
ng
!
CO
2
gas
b
ott
l
es may
b
urst or exp
l
o
d
e at
hi
g
h
temperature. Do not
use naked flames in the vicinity of the gas bottles. Store gas bottles
at lower than 5
0
°
C
in a well-ventilated location. Prevent water from
p
enetrating, as well as backflow into the gas bottles. It is essential
that you read the safety notes and regulations of the gas suppliers.
CO
2
an
d
N
2
are not dan
g
erous substances in terms of the German Ordinance on Hazardous
Substances (GefStoffV). You should nevertheless familiarise yourself with the applicable safety
re
g
u
l
ations prior to
h
an
dl
in
g
suc
h
g
as
b
ott
l
es
.

8
S
afety regulations
1.
3
R
equirements of the
o
peratin
g
personne
l
T
he incubator may only be operated and maintained by persons who are of le
g
al a
g
e, and
who have received instructions for the incubator. Personnel who are to be trained
,
instructed
o
r w
h
o are un
d
er
g
oin
g
g
enera
l
trainin
g
may on
l
y
b
e active on t
h
e incu
b
ator un
d
er t
h
e con
-
tinuous supervision of an experienced person.
T
he incubator may only be transported by persons (fork-lift truck, manual pallet jack), who are
trained for this work and who know the correspondin
g
safety re
g
ulations
.
R
epairs may only be performed by qualified electricians. In this case the re
g
ulations in the
s
eparate service manua
l
must
b
e o
b
serve
d
.
1.4
R
esponsibility of the owner
T
h
e
o
wn
e
r
o
f th
e
incubat
o
r
Ź
is responsible for the flawless condition of the incubator and for the incubator bein
g
oper-
ated in accordance with its intended use (see pa
g
e
14
);
Ź
is responsible for ensurin
g
that persons who are to operate or service the incubator are
q
ualified to do this, have received instructions about the incubator and are familiar with
t
his operatin
g
manual
;
Ź
m
ust
k
now a
b
out t
h
e app
l
ica
bl
e re
g
u
l
ations, requirements an
d
wor
k
protection re
g
u
l
a-
t
ions, and train staff acordin
g
ly
;
Ź
is responsible for ensurin
g
that unauthorised persons have no access to the incubator
;
Ź
is responsible for ensurin
g
that the maintenance plan is adhered to and that maintenance
and repair work is properly carried out (see pa
g
e
59
);
Ź
e
nsures, for example throu
g
h correspondin
g
instructions and inspections, that the incuba
-
t
or an
d
its surroun
d
in
g
s are
k
ept c
l
ean an
d
ti
d
y;
Ź
is responsible for ensurin
g
that personal protective clothin
g
is worn by operatin
g
person-
n
el, e.
g
. work clothes, safety shoes, protective
g
loves.
1.5
Ch
an
g
es an
d
c
o
nv
e
rs
io
n
s
N
o independent conversions or alterations may be made to the incubator. No parts may be
added or inserted which have not been approved by the manufacturer.
I
ndependent conversions or alterations result in the EC declaration of conformity (see pa
g
e
15) losin
g
its validity, and the incubator may no lon
g
er be operated.
T
he manufaturer is not liable for any dama
g
e, dan
g
er or injuries that result from independent
c
onversions or alterations, or from non-observation of the re
g
ulations in this manual
.
1.
6
Be
havi
o
ur in cas
e
o
f malfuncti
o
ns and irre
g
u
l
aritie
s
T
he incubator may only be used when in a flawless condition. If you as the operator notice
irre
g
ularities, malfunctions or dama
g
e, immediately put the incubator out of service (see
Ch
apter 1.
8
) and inform your superiors.
Y
ou can find information on eliminatin
g
malfunctions from pa
g
e
38
.

9
S
afety regulations
1.7
What t
o
d
o
in cas
e
o
f acci
de
nts
1.
K
eep ca
l
m. Act reso
l
ute
l
y an
d
wit
h
consi
d
eration. Pay attention to your own
s
afety.
2
. Switch off the incubator and close the valves on the
g
as bottle.
3
.
C
a
ll
a
do
ct
o
r.
4
.
I
nitiate first aid measures. If available: Call a trained first aid helper.
In cas
e
of contact wit
h
CO
2
2
to the eyes and skin:
y
R
inse eyes out with water for at least 15 minutes. With cold burns, rinse with water
fo
r at l
e
ast 15 minut
e
s. C
o
v
e
r
o
v
e
r in a st
e
ril
e
mann
e
r. Call a d
o
ct
o
r.
Inhaling CO
g
2
2
or N
2
2
N
:
H
i
g
h concentrations can cause suffocation. Symptoms may include a loss of mobility and
c
onsciousness. The victim is not aware of suffocatin
g.
L
ow concentrations of C
O
2
can cause acce
l
erate
d
b
reat
h
in
g
an
d
h
ea
d
ac
h
es
.
A
nyone affected should breathe fresh air, usin
g
a breathin
g
device independent of recircu-
latin
g
air. Keep the person warm and calm. Call a doctor. In case of respiratory arrest, use
artificial respiration
.
If gas is escaping:
gpg
L
eave the room immediately, warn others and ventilate the room. If you re-enter the room,
u
se an autonomous breathin
g
device (independent of ambient air) if it has not been estab-
l
is
h
e
d
t
h
at t
h
e atmosp
h
ere is
h
arm
l
ess.
1.
8
Switchin
g
off incubator in an
e
m
e
r
gen
c
y
Ź
P
ush main switch on front side of appliance
(
Fig
. 1).
Ź
Cl
ose t
h
e va
l
ves on t
h
e
g
as
b
ott
l
e.
F
ig
. 1 Switc
h
off incu
b
ator
by
pressing the main switch
g
y

1
0
D
esign and Functio
n
2.
Desi
g
n an
d
Functio
n
2
.1 Des
ign
4
1
2
3
14
13
11
12
9
10
8
7
6
5
18
17
16
15
F
i
g. 2 Design of INCO incubators
1
Controller/control panel (see page
27
)
7
2
Set key
3
Push/turn control
y
4
C
onn
e
ctions on t
he
r
e
ar of t
he
ov
e
n
(
see page
13
)
3
5
Heating ri
b
s (see page
11
)
6
Pt100 temperature sensors
7
Humidity sensor
p
8
Gl
a
ss
d
oor
y
9
Ch
am
be
r s
e
a
l
10
Sliding shelf
1
1
D
oor s
e
a
l
g
1
2
Ventilator/inlet opening
13
Heating ribs
1
4
Adjustable feet
g
g
15
Nameplate (beneath door, see page
j
15
)
5
16
Door knob (see page
p
p
25
)
5
17
Doo
r
18
C
h
ip car
d
rea
d
er

1
1
D
esign and Functio
n
2.2
F
unct
ion
T
he air in the incubator is heated up by an all-round heater with a lar
g
e surface (
Fig
.
3
, No. 1).
Th
e C
O
2
an
d
/or
N
2
g
as is introduced into the workin
g
chamber via a sterile filter. Because CO
2
has a much hi
g
her specific wei
g
ht than air, the
g
as is let into the workin
g
chamber above the
interior fan (2). The turbulence-free interior ventilation (3) ensures a uniform distribution of
t
h
e
g
ases, creatin
g
a
h
omo
g
enous atmosp
h
ere
.
I
n the basic version, humidification takes place by means of water trays (4). To avoid uncon-
trolled condensation, dosed fresh air is piped into the interior. If the appliance is equipped
with a humidity module, humidification takes place via a hot-air
g
enerator, which allows
water to eva
p
orate at a set rate. The sterile hot air is let into the interior above the fan and is
mixed with the air current (5).
11
1
1
1
1
4 4
2
5
3
1
F
i
g. 3 How IN
CO
incu
b
ators wor
k
2
.3
O
ptiona
l
e
x
t
ras
2
.3.1
B
as
i
c equ
i
pment
Ź
E
lectronic fuzzy-supported PID process controller with pulse width modulation and per-
m
anent performance adjustments and time-savin
g
self-dia
g
nosis system to quickly locate
e
rrors (see pa
g
e
39
)
Ź
A
ll-round heater with lar
g
er surface area, with additional thermal conduction layer (see
Fig
.
3
)
Ź
C
apacitive
h
umi
d
ity sensor
Ź
H
umidity control (fresh air via sterile filter) ensures that setpoint humidity is quickly
r
eached and
g
uarantees short recovery times, while avoidin
g
condensation formatio
n
Ź
H
omo
g
enous atmosp
h
ere an
d
temperature
d
istri
b
ution t
h
rou
gh
encapsu
l
ate
d
, tur
b
u-
lence-free ventilation system
Ź
S
TERICard for fully automatic sterilisation process control for hot air sterilisation of appli
-
ance, includin
g
sensors and fan rotor (see pa
g
e
49
)

1
2
D
esign and Functio
n
Ź
D
i
g
ita
l
ise
d
e
l
ectronic C
O
2
contro
l
wit
h
automatic zero position, NDIR measurin
g
system
with self-dia
g
nosis system and acoustic error display, air pressure compensatio
n
Ź
L
an
g
ua
g
e settin
g
s (see pa
g
e
44
)
Ź
Al
p
h
anumeric text
d
isp
l
ay
Ź
I
nte
g
rated week time switch with
g
rup function (e.
g
. each workin
g
day) (see pa
g
e
3
1
)
Ź
R
etractin
g
push-turn control for simple operation of appliance (see pa
g
e
26
)
Ź
T
wo separate Pt100 temperature sensors DIN Class A in a 4-wire circuit for control and
m
on
i
tor
i
n
g
Ź
D
i
g
ital monitorin
g
control for overtemperature, undertemperature and automatic
s
etpoint followin
g
(ASF) (see pa
g
e
48
)
Ź
M
echanical temperature limiter (TB protection class 1, see pa
g
e
4
5
)
Ź
M
onitorin
g
relay to switch off heater in case of erro
r
Ź
O
ptica
l
a
l
arm
d
isp
l
a
y
Ź
A
coustic si
g
nal messa
g
es if temperature or C
O
2
l
imits are crosse
d
, w
h
en
d
oor is open an
d
if
g
as bottle is empty (see pa
g
e
38
)
Ź
C
alibration of temperature, humidity, CO
2
an
d
O
2
possi
bl
e on t
h
e
d
evice wit
h
out a sepa-
r
ate PC (see from pa
g
e
50
)
2
.3.
2
O
ptiona
ll
y avai
l
a
bl
ea
dd
iti
o
na
l
m
od
u
le
s
C
omfort mo
d
u
le
:
Ź
T
wo
g
as connections with quick release connectors (see pa
g
e 1
3
)
Ź
A
utomatic switch-over of
g
as bottles
Hygiene module
yg
Ź
E
lectro-polished, seamless laser-welded chamber
C
ommunication modul
e
Ź
L
o
gg
in
g
option of temperature, CO2 and relative humidity via computer/laptop
Ź
I
nternal lo
g
memory with 1024 kB as rin
g
memory for all setpoint and actual values, errors
an
d
settin
g
s in rea
l
time an
d
wit
h
d
ate,
l
o
gg
in
g
approx. 3 mont
h
s at 1 minute stora
g
e
interval (see pa
g
e
58
)
Ź
P
arallel printer port (PCL3-compatible) for printin
g
out lo
g
data (see pa
g
e 1
3
an
d
pa
g
e
43
)
Ź
o
ptionally USB, Ethernet, RS-232 or RS-485 interface (see from pa
g
e
5
5
)
CO
2
2
mo
d
u
le
Ź
E
xtended adjustment ran
g
e from 0 to 20 %
Ź
3
-point calibration (5 %, 10 %, 15 % CO
2
)
O
2
2
mo
d
u
le
Ź
C
ontrol of oxy
g
en concentration throu
g
h the introduction of nitro
g
en (
N
2
)
; adjustment
r
an
g
e 1
%
to 2
0
%
O
2
(not in combination with Comfort or Premium module
)
Pr
e
mium mo
d
u
le
Ź
I
ncludes comfort, hy
g
iene, communication and C
O
2
m
od
u
le
s
Humidity module
y
Ź
A
ctive microprocessor humidification and dehumidification control (40-97 % rh
)

1
3
D
esign and Functio
n
2.4
Mat
e
ria
l
F
or the outer housin
g
, MEMMERT uses stainless steel (W.St.No. 1.4016), and for the interior,
s
tainless steel (W.St.No. 1.4301), which stands out throu
g
h its hi
g
h stability, optimal hy
g
ienic
properties and corrosion-resistance towards many (but not all!) chemical compounds (caution
for example with chlorine compounds). The chamber load for the appliance must be carefully
ch
ec
k
e
d
wit
h
respect to c
h
emica
l
compati
b
i
l
ity wit
h
t
h
e materia
l
s mentione
d
.
A
material resistance table can be requested from the manufacturer.
2
.5
El
ectrica
l
equipment
Ź
O
peratin
g
volta
g
e: See nameplate (pa
g
e 15), 50/60 Hz
Ź
C
urrent consumption: See nameplate (pa
g
e 15
)
Ź
P
rotection class 1, i.e. operatin
g
insulation with safety earth terminal in accordance with
E
N 61
0
1
0
Ź
P
rotect
i
on type IP 20 acc. to EN 60 529
Ź
I
nterference-suppressed acc. to EN 55011 class B
Ź
A
ppliance fuse: Fusible link 250 V/15 A quick-blow
Ź
T
he temperature controller is protected with a miniature fuse 100 mA (200 mA at 115 V)
Ź
F
or mo
d
e
l
s wit
h
a
h
umi
d
ity mo
d
e
l
t
h
e CO
2
contro
ll
er is protecte
d
wit
h
a 6.3 A miniature
f
us
e
2
.
6
Co
nn
e
ct
io
n
s
3
4
5
6
2
1
H
2
O
7N2
In
Fig. 4
Connections on rear of a
ppl
iance
1
Water connection (only for model with humidity module)
pp
pp
2
Mains lead
3
USB connection (only for models with communication or premium modules;
alternatively Ethernet, RS-232 or RS-485 connection) (details from page
yp
yp
55
)
5
4
Printer connection (only for models with communication or premium modules) (see page
ypg
4
3
)
3
5
CO
2
connection to main gas bottle
6
CO
2
connection to reserve gas bottle (only for models with CO
g
2
or premium modules)
7
N
2
N
connection (quick-release connector, only if equipped with O
2
gy
gy
2
2
2
mo
d
u
l
e
)
p

14
D
esign and Functio
n
2
.
6
.1
Ele
ctrica
l
c
o
nn
e
cti
on
O
bserve the country-specific re
g
ulations when makin
g
connections (e.
g
. in Germany DIN VDE
0
100 with residual current device).
T
his appliance is intended for operation on an electrical power system with a system imped-
anc
e
Z
max
at the point of transfer (service line) of a maximum of 0.292 ohm. The operator must
x
e
nsure t
h
at t
h
e incu
b
ator is operate
d
on
l
y on an e
l
ectrica
l
power system t
h
at meets t
h
ese
requirements. If necessary, you can ask your local ener
g
y supply company what the system
impe
d
ance is.
2
.
6
.
2
C
onnection of external appliances
O
nly appliances may be connected externally (dependin
g
on the model, USB, RS 232, RS 485,
E
thernet, printer) whose interfaces comply with the requirements for safety extra-low volta
g
e
(
e.
g
. PC).
2
.
6
.3 Gas c
o
nn
e
ct
ion
Th
e oven can
b
e connecte
d
wit
h
t
h
e supp
l
ie
d
compresse
d
air
h
ose via a pressure re
g
u
l
ator
with
g
as bottle monitor (DIN 8546) to a CO
2
compresse
d
g
as
b
ott
l
e or
d
irect
l
y to a centra
l
CO
2
g
as supp
l
y.
F
or models with the C
O
2
or premium modules, two
g
as bottles with quick release connectors
c
an be connected. If e
q
ui
pp
ed with the O
2
mo
d
u
l
e
,
an N
2
g
as
b
ott
l
e can
b
e connecte
d
instea
d
o
f a second C
O
2
g
as bottle (
Fig
. 4).
Th
e pre-pressure must not excee
d
1.2
b
ar. A va
l
ue
b
etween 0.8 an
d
1
b
ar is consi
d
ere
d
to
b
e
an i
de
a
l
va
l
u
e
.
2
.
6
.4 Wat
e
r c
o
nn
e
ct
ion
F
or mo
d
e
l
s wit
h
a
h
umi
d
ity mo
d
u
l
e, t
h
e incu
b
ator can
b
e connecte
d
wit
h
t
h
e supp
l
ie
d
h
ose
to t
h
e a
l
so supp
l
ie
d
water supp
l
y tan
k
.
2
.7 Int
e
n
ded
us
e
I
NCO incubators may only be used for incubatin
g
cell cultures or similar. Any other use is
improper, an
d
may resu
l
t in
h
azar
d
s an
d
d
ama
g
e.
The
incu
b
at
o
rs ar
e
n
ot
e
xplosion-proof (they do not comply with workplace health & safety
re
g
ulation VBG 24). The chambers may only be loaded with materials and substances which
c
annot pro
d
uce any toxic or exp
l
osive vapours at temperature ran
g
es up to 50 ºC, an
d
w
h
ic
h
t
h
emse
l
ves cannot exp
l
o
d
e,
b
urst or i
g
nite.
T
he incubators may not be used for dryin
g
, vaporisin
g
and brandin
g
paints or similar materi
-
als, the solvents of which could form an explosive mixture when combined with air. If there is
any doubt as to the composition of materials, they must not be loaded into the incubator. Po-
tentially explosive
g
as-air mixtures must not be produced, either in the interior of the chamber
o
r in the direct vicinity of the appliance.
T
he incubator may not be used for sterilisation purposes. It is not a steriliser with respect to
t
he
Law
o
n M
ed
ica
l
D
e
vic
e
s.
O
nly
g
as bottles with a pressure re
g
ulator may be connected to the
g
as connections of the
o
ven. Intro
d
ucin
g
ot
h
er
g
ases or materia
l
s t
h
an C
O
2
o
r
N
2
is not permitte
d
.

1
5
D
esign and Functio
n
2
.7.1
I
ntended use of the INC
O
med
F
or INCOmed incubators, which are subject to the 93/42/EEC
g
uideline (Council Directive on
the approximation of the laws of the Member States relatin
g
to medical devices), the intended
u
s
e
is d
e
fin
e
d as f
o
ll
o
ws:
T
he C
O
2 incubator INC
O
med is intended for the creation and maintenance of constant envi-
ronmental conditions for application in the field of in vitro fertilisation (IVF), especially for the
incubation of oocytes, spermatozoa and zy
g
otes in special culture dishes for IVF application as
well as for
g
ene expression and the biosynthesis of RNA and proteins.
2.
8
EC
De
clarati
o
n
o
f
C
onformit
y
Y
ou can download the EC declaration of conformity of the appliance online:
E
n
g
lish: http://www.memmert.com/en/service/downloads/ce-statement
/
German: http://www.memmert.com/de/service/downloads/e
g
-konformitaetserklaerun
g/
2.
9
Des
ig
nat
i
on (nameplate
)
T
he nameplate (
Fig
. 5) provides information about the appliance model, manufacturer and
technical data. It is attached to the front of the appliance, on the ri
g
ht beneath the door (see
page
1
0
).
Typ: INC 108 F.-Nr.: 0109.0088
230 V~ 4.4 A 50/60 Hz 1000 W
DIN12880-Kl.3.1 Nenntemp.: 50 °C
1
2
3
4
5
6
10
8
9
7
F
i
g. 5 Nameplat
e
1
Typ
e
d
esi
g
nation
2
Operating voltage
yp g
3
Applied standard
pgg
gg
4
Protection type
5
CE con
f
ormity
yp
6
A
dd
r
e
ss of manufactur
er
7
D
isposa
l
note
8
T
em
p
erature ran
ge
9
Connection / performance values
pg
g
10
Factory number
p

1
6
D
esign and Functio
n
2.1
0
T
e
c
h
nica
l
d
ata
Model
* See Fig. 6 on page 17. 108 153 246
C
hamber width A* [mm]
5
6
0
4
8
0
6
4
0
C
hamber hei
g
ht B* [mm
]
4
8
0
6
4
0
6
4
0
C
hamber depth C*
[
mm
]
400
500
600
A
ppliance width D* [mm] 71
0
6
3
0
7
90
A
ppliance hei
g
ht E* (varies
d
ue to adjustable feet) [mm] 77
8
9
2
0
9
3
8
A
ppliance depth F* (includ-
in
g
door handle) [mm
]
590
690
7
90
C
hamber volume [litres
]
1
0
815
3
2
4
6
Wei
g
ht [k
g
] 7
0
90
11
0
P
erformance [W
]
1
000
15
00
2000
M
ax. number of slidin
g
s
helves half size / full size
-/4
-/6
2 x 6
/
6
M
ax. load per slidin
g
shelf
[
k
g]
15 15 15
M
ax. load per appliance [k
g
]
40
40
60
T
emperatur
e
T
emperature recordin
g
by means of Pt100 in a 4-wire circuit
Ad
justment ran
g
e: Norma
l
mo
d
e: 20 °C to 50 °C
Sterilisation mode: 160 °C (4 hours) via STERICard
Ad
justment precision: 0.1 °C
C
ontrol ran
g
e: from 8 °C above room temperature to 50 °C
Variation (time): max. ±0,1 °C at 37 °C
Variation (spatial): max. ±0.3 °C at 37 °C
H
umi
d
ity
Th
e re
l
ative
h
umi
d
ity in t
h
e c
h
am
b
er is measure
d
b
y a capac-
itive
h
umi
d
ity sensor an
d
d
isp
l
aye
d
d
i
g
ita
ll
y in percent.
T
he measurement precision of the humidity sensor is 1 % rh
Ź
A
djustment ran
g
e: 88 to 97 % rh (
fo
r m
o
d
e
ls with
h
umi
d
ity mo
d
u
le
4
0 to 97 % rh)
Ź
Ad
justment precision: 1 % r
h
Ź
D
isp
l
ay ran
g
e: 10 to 98 % r
h
Ź
Variation (time): max. ±1 % rh

17
D
es
i
gn an
d
Funct
i
o
n
Model
* See Fig. 6 on page 17. 108 153 246
2
Th
e C
O
2
content is
d
etermine
d
t
h
rou
gh
an NDIR measur-
in
g
system, contro
ll
e
d
constant
l
y
b
y a microprocessor an
d
d
isp
l
aye
d
d
i
g
ita
ll
y in percent
Ź
A
djustment ran
g
e: 0 to 10 % (for models with CO
2
o
r
premium modules 0 to 20 %)
Ź
Ad
justment precision: 0.1 %
Ź
Variation (time): max. ±0.1 % rh
Ź
Variation (spatial): max. ±0.3 %
O
2
(only if equipped with O
2
module
)
Th
e
O
2
content is
d
etermine
d
wit
h
a
l
on
g
-
l
astin
g
, mainte-
nance-free zirconium dioxide sensor, constantly controlled by
a microprocessor an
d
d
isp
l
aye
d
d
i
g
ita
ll
y in percent
Ź
Ad
justment ran
g
e: 1 to 20 %
Ź
Ad
justment precision: 0.1 %
Ź
Variation (time): max. ±0.1 %
Ź
Variation (spatial): max. ±0.3 %
D
A75 75 38
38
F
C
202
B
76
E
8
12
F
i
g. 6 Dim
e
nsions of IN
CO
incu
b
ators

1
8
D
esign and Functio
n
2.11
A
m
b
i
e
nt c
o
n
d
iti
o
ns
Ź
T
he incubator may only be used in enclosed rooms and under the followin
g
environmental
co
n
d
iti
o
ns
:
A
m
b
ient temperature: 5 ºC to 35 ºC
H
umi
d
ity: max. 80 % not con
d
ensin
g
D
e
g
ree of pollution: 2
A
ltitude of installation max. 3
,
000 m above sea level
Ź
T
he incubator may not be used in areas where there is a risk of explosions. The ambient air
m
ust not contain any explosive dusts,
g
ases, vapours or
g
as-air mixtures. The incubator is
n
ot ex
p
losion-
p
roof.
Ź
H
eavy dust production or a
gg
ressive vapours in the vicinity of the appliance could lead to
s
e
d
imentation in t
h
e c
h
am
b
er interior an
d
as a consequence, cou
ld
resu
l
t in s
h
ort circuits
o
r dama
g
e to electrical parts. For this reason, sufficient measures should be taken to
p
revent lar
g
e clouds of dust or a
gg
ressive vapours from developin
g
.
2
.1
2
A
cc
e
ss
o
ri
e
s inc
l
u
ded
For incubators with basic fittings:
g
Ź
T
wo (INCO 108) or three (INCO 153 and 246) slidin
g
shelves
Ź
Gas pressure
h
os
e
ŹWater tray (one for INCO 108 and 153 incubators, two for INCO 246 incubators)
Ź
S
terilisation chi
p
car
d
Additionally, for models with humidity module:
y, y
Ź
Water supp
l
y tan
k
an
d
connection
h
os
e
Additionally, for models with CO
y,
2
2
or premium module:
p
Ź
S
econ
d
g
as pressure
h
ose wit
h
quic
k
-re
l
ease connecto
r
Additionally, for models with O
y,
2
2
mo
d
u
le
:
Ź
S
econd
g
as pressure hose with quick-release connecto
r

1
9
D
elivery, Transport and Setting Up
3
.
D
e
l
ivery,
T
ransport an
d
Sett
i
n
g
Up
3
.1 Safety re
g
ulations
W
arn
i
ng!
You may injure your hands or feet when transporting and
i
nstalling the incubator. You should wear protective gloves
and work
s
ho
es
.
W
arn
i
ng
!
B
ecause of the weight of the incubator, you could injure yourself if
you try to lift it on your own.
I
f possible, only transport the incubator with a fork-lift truck or
m
anua
l
pa
ll
et
j
ac
k
. T
h
e
i
ncu
b
ator may on
l
y
b
e move
d
us
i
ng a means
of transport by persons who have the required qualification for this
(
e.g. fork-lift truck licence). This incubator may not be transported
wi
t
h a
c
ran
e
.
I
f the incubator has to be carried, at least two people are required
for models 108 and 153, and at least four people for model 246.
W
arning
!
The incubator could fall over and seriously injure you. Never tilt the
i
ncu
b
ator an
d
transport
i
t on
l
y
i
n an upr
i
g
h
t pos
i
t
i
on.
3
.
2
T
ranspor
t
Th
e incu
b
ator can
b
e transporte
d
in t
h
ree ways:
Ź
with a fork-lift truck; move the forks of the truck entirely under the incubator
Ź
o
n t
h
e manua
l
pa
ll
et jac
k
.
Ź
b
y carryin
g
; to do this, at least two people are required for models 108 and 153, and four
p
eople for model 246. Read the wei
g
ht information detailed on pa
g
e 1
6
.
3
.
3
D
e
l
ivery
Th
e incu
b
ator is
d
e
l
ivere
d
in car
db
oar
d
pac
k
a
g
in
g
on a car
db
oar
d
pa
ll
et.
3
.
3
.1 Unpac
k
in
g
1.
R
emove cardboard packa
g
in
g
or cut open carefully alon
g
an ed
g
e
.
2
.
L
ift up incubator from pallet and put down on the appliance feet.
3
.3.
2
C
heckin
g
for completeness and transport
d
ama
ge
Ź
Ch
ec
k
t
h
e
d
e
l
ivery note to ensure t
h
at t
h
e
d
e
l
ivery is comp
l
ete.
Ź
C
heck the inside and outside of the incubator for dama
g
e.
I
f you notice deviations from the delivery note, dama
g
e or irre
g
ularities, do not put the incu-
bator into operation, but inform the haula
g
e company and the manufacturer
.
3
.
3
.
3
D
isposin
g
of packa
g
in
g
materia
l
D
ispose of the packa
g
in
g
material (cardboard) in accordance with the effective le
g
al disposal
re
g
ulations for cardboard packa
g
in
g
in your country
.

2
0
D
elivery, Transport and Setting Up
3
.
3
.4 Stora
g
e after delivery
I
f the incubator is initially to be stored after delivery: Read the stora
g
e conditions from pa
g
e
6
1.
3
.4
S
etup
T
he incubator can be placed either on the
g
round or on a table (work surface). When doin
g
t
h
is, ensure t
h
at t
h
e app
l
iance is positione
d
exact
l
y
h
orizonta
ll
y.
The
insta
ll
ati
o
n sit
e
must
be
level and able to reliably carry the wei
g
ht of the incubator (see pa
g
e 1
6
)
. Do not place the ap-
pliance on an inflammable surface
.
A
power connection must
b
e avai
l
a
bl
e at t
h
e insta
ll
ation site in accor
d
ance wit
h
t
h
e connec-
tion data on the nameplate (see pa
g
e 15).
T
h
e
distanc
e
b
e
tw
ee
n th
e
wall and th
e
r
e
ar
o
f th
e
chamb
e
r must b
e
at l
e
ast 15 cm. Th
e
cl
e
ar-
ance from the ceilin
g
must not be less than 20 cm and the side clearance from the wall not
less than 8 cm (
Fig
. 7). Sufficient air circulation in the vicinity of the chamber must be
g
uaran
-
t
ee
d at all tim
e
s.
FP

FP

FP

FP

F
i
g.
7
Minimum c
l
earance from wa
ll
s an
d
cei
l
ing