Metrohm Inline Ultrafiltration 2 User manual

IC equipment
IC equipment: Inline Ultrafiltration 2 - push mode
Manual
8.0108.8026EN/2020-05-22

Metrohm AG
CH-9100 Herisau
Switzerland
Phone [phone]
Fax [phone]
[email protected]
www.metrohm.com
IC equipment
IC equipment: Inline Ultrafiltration 2
- push mode
6.05330.110
Manual
8.0108.8026EN/2020-05-22

Technical Communication
Metrohm AG
CH-9100 Herisau
[email protected]
This documentation is protected by copyright. All rights reserved.
This documentation has been prepared with great care. However, errors
can never be entirely ruled out. Please send comments regarding possible
errors to the address above.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents
IC equipment: Inline Ultrafiltration 2 - push mode ￭￭￭￭￭￭￭￭ III
Table of contents
1 Introduction 1
1.1 Description of the IC equipment: Inline Ultrafiltration 2
- push mode .......................................................................... 1
1.2 About the documentation ................................................... 1
1.3 Symbols and conventions .................................................... 2
2 Mode of operation of ultrafiltration 3
2.1 Mode of operation of the push mode ............................... 3
2.2 Mode of operation of the pull mode ................................. 3
2.3 Sample volume ...................................................................... 4
3 Overview 5
3.1 Parts of the IC equipment: Inline Ultrafiltration 2 - push
mode ...................................................................................... 5
3.2 Flow diagram of the IC equipment: Inline Ultrafiltration
2 - push mode ....................................................................... 7
3.3 Components of the ultrafiltration cell ................................ 8
3.4 Connectors of the ultrafiltration cell ................................... 9
4 Installation 10
4.1 Preparing the ultrafiltration cell ........................................ 10
4.2 Connecting the ultrafiltration cell ..................................... 14
4.3 Inserting the ultrafiltration cell ......................................... 17
4.4 Purging the ultrafiltration cell ........................................... 18
5 Operation 19
5.1 Ultrafiltration time and sample volume ............................ 19
5.2 Selecting the filtration membrane .................................... 19
6 Maintenance 21
6.1 Service life of the filtration membrane ............................. 21
6.2 Replacing the filtration membrane ................................... 22
7 Technical specifications 24
7.1 Ultrafiltration cell 2 (6.02729.110) ................................... 24
7.2 Filtration membrane 1 (6.2714.020) ................................. 24

Table of contents ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
IV ￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration 2 - push mode
8 Accessories 25
Index 26

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures
IC equipment: Inline Ultrafiltration 2 - push mode ￭￭￭￭￭￭￭￭ V
Table of figures
Figure 1 IC equipment: Inline Ultrafiltration 2 - push mode – Parts .................. 5
Figure 2 Flow diagram of the IC equipment: Inline Ultrafiltration 2 - push
mode ................................................................................................ 7
Figure 3 Ultrafiltration cell – Parts .................................................................... 8
Figure 4 Ultrafiltration cell – Connectors ......................................................... 9
Figure 5 push mode – Connecting the ultrafiltration cell ................................ 14

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
IC equipment: Inline Ultrafiltration 2 - push mode ￭￭￭￭￭￭￭￭ 1
1 Introduction
1.1 Description of the IC equipment: Inline Ultrafiltra-
tion 2 - push mode
The IC equipment: Inline Ultrafiltration 2 - push mode (6.05330.110) con-
tains all accessories required for Inline Ultrafiltration directly before injec-
tion.
The main component of the IC equipment: Inline Ultrafiltration 2 - push
mode is the ultrafiltration cell. This cell is suitable for the filtration of sam-
ples with high requirements regarding filtration effectiveness and sample
throughput.
2 peristaltic pumps are needed in addition to the IC equipment: Inline
Ultrafiltration 2 - push mode for pumping the sample and the filtrate.
1.2 About the documentation
This manual describes the correct assembly and maintenance of the IC
equipment: Inline Ultrafiltration 2 - push mode, the installation of the
capillary connections and the assembly of the holder to the sample
changer.
The installation of the peristaltic pump is not described in this manual. The
installation of the peristaltic pump is described in the respective manuals
for the ion chromatograph or for the sample changer.
CAUTION
Please read through this documentation carefully before putting the IC
equipment: Inline Ultrafiltration 2 - push mode into operation. The doc-
umentation contains information and warnings which the user must
follow in order to ensure safe operation of the IC equipment: Inline
Ultrafiltration 2 - push mode.

1.3 Symbols and conventions ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
2￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration 2 - push mode
1.3 Symbols and conventions
The following symbols and formatting may appear in this documentation:
Cross-reference to figure legend
The first number refers to the figure number, the sec-
ond to the instrument part in the figure.
Instruction step
Carry out these steps in the sequence shown.
Method Dialog text, parameter in the software
File ▶ New Menu or menu item
[Next] Button or key
WARNING
This symbol draws attention to a possible life-threat-
ening hazard or risk of injury.
WARNING
This symbol draws attention to a possible hazard due
to electrical current.
WARNING
This symbol draws attention to a possible hazard due
to heat or hot instrument parts.
WARNING
This symbol draws attention to a possible biological
hazard.
CAUTION
This symbol draws attention to possible damage to
instruments or instrument parts.
NOTE
This symbol highlights additional information and
tips.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Mode of operation of ultrafiltration
IC equipment: Inline Ultrafiltration 2 - push mode ￭￭￭￭￭￭￭￭ 3
2 Mode of operation of ultrafiltration
One peristaltic pump delivers a continuous flow of sample solution at a
high rate through the sample chamber of the ultrafiltration cell. The sam-
ple flows along the filtration membrane to the sample outlet and into the
waste container.
At the same time, a second peristaltic pump generates a vacuum in the
filtrate chamber of the ultrafiltration cell, thus aspirating the sample solu-
tion through the filtration membrane. The filtrate reaches the sample loop
and is then injected.
20 to 25% of the original sample solution volume is analyzed as filtrate.
The remainder flows into the waste container.
There are 2 options to connect the ultrafiltration cell. The push mode (see
chapter 2.1, page 3) and the pull mode (see chapter 2.2, page 3).
2.1 Mode of operation of the push mode
The basic principle of the push mode is that the sample is delivered by
pushing it.
In push mode, one peristaltic pump is installed between the sample
changer and the sample inlet of the ultrafiltration cell. The other peristaltic
pump is installed between the filtrate outlet and the injector. The sample
flows through both peristaltic pump tubings before analysis. As the peri-
staltic pump tubings have a high volume, the required sample volume is
higher in the push mode than in the pull mode (see chapter 2.3, page
4).
2.2 Mode of operation of the pull mode
The basic principle of the pull mode is that the sample is delivered by pull-
ing it.
In pull mode, one peristaltic pump is installed between the sample outlet
of the ultrafiltration cell and the waste container. The other peristaltic
pump is installed between the injector and the waste container. Rinsing
the additional volume of the peristaltic pump tubings is not necessary.
Thus, the required sample volume is lower in the pull mode than in the
push mode (see chapter 2.3, page 4).

2.3 Sample volume ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
4￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration 2 - push mode
2.3 Sample volume
The sample volume depends on the following factors:
￭Volume of the sample needle
The lower the volume of the sample needle, the smaller the required
sample volume.
￭Length of the capillaries
The shorter the length of the capillaries, the smaller the required sam-
ple volume.
￭Inner diameter of the capillaries
The smaller the inner diameter of the capillaries, the smaller the
required sample volume.
￭Volume of the peristaltic pump tubings
The lower the volume of the peristaltic pump tubings, the smaller the
required sample volume.
￭Volume of the ultrafiltration cell
The lower the volume of the ultrafiltration cell, the smaller the required
sample volume.
￭Volume of the sample loop
The lower the volume of the sample loop, the smaller the required
sample volume.
￭Ratio of the flow rate on the sample side to the flow rate on the filtra-
tion side
On the sample side, a high flow rate is required to pump a high sample
volume through the sample chamber. On the filtration side, a lower
flow rate is required as less filtrate needs to be pumped through the
filtrate chamber than sample through the sample chamber.
The smaller the ratio of the flow rate on the sample side is to the flow
rate on the filtration side, the smaller the required sample volume.
The pull mode and the push mode differ with regards to the length of the
capillaries and the volume of the peristaltic pump tubings. The volume of
the peristaltic pump tubings in particular carries weight.
Due to the additional volume of the peristaltic pump tubings, the required
volume is considerably higher in the push mode than in the pull mode.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Overview
IC equipment: Inline Ultrafiltration 2 - push mode ￭￭￭￭￭￭￭￭ 5
3 Overview
3.1 Parts of the IC equipment: Inline Ultrafiltration 2 -
push mode
Figure 1 IC equipment: Inline Ultrafiltration 2 - push mode – Parts
Table 1 Parts of the IC equipment: Inline Ultrafiltration 2 - push mode
Article number Article name Number
6.2714.020 Filtration membrane 1 1
6.1803.040 PTFE capillary, 0.5 mm ID / 1 m 2
6.02729.110 Ultrafiltration cell 2 1
6.1831.160 PEEK capillary, 0.5 mm ID / 70 cm 1
6.2831.010 Plastic tweezers 1
6.2621.030 Hex key 4 mm 1

3.1 Parts of the IC equipment: Inline Ultrafiltration 2 - push mode ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
6￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration 2 - push mode
Article number Article name Number
6.02057.030 Filtration cell holder 1
6.2744.160 Pump tubing connection with locking nut 1
6.2744.034 Coupling olive/UNF 10/32, 2x 1
6.2744.070 Pressure screw, short, 5x 1
6.2744.000 Pressure screw PVDF, 5x 1
6.1826.320 Pump tubing LFL (orange/yellow), 3 stoppers 1
6.1826.390 Pump tubing LFL (yellow/yellow), 3 stoppers 1
6.1803.050 PTFE capillary, 0.5 mm ID / 20 cm 2

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Overview
IC equipment: Inline Ultrafiltration 2 - push mode ￭￭￭￭￭￭￭￭ 7
3.2 Flow diagram of the IC equipment: Inline Ultrafiltra-
tion 2 - push mode
Figure 2 Flow diagram of the IC equipment: Inline Ultrafiltration 2 - push mode
16.1831.160
PEEK capillary, 0.5 mm ID / 70 cm
26.1803.050
PTFE capillary, 0.5 mm ID / 20 cm
36.1803.040
PTFE capillary, 0.5 mm ID / 1 m
46.1803.050
PTFE capillary, 0.5 mm ID / 20 cm
56.1803.040
PTFE capillary, 0.5 mm ID / 1 m
6PTFE capillary, 0.5 mm ID
A PTFE capillary, 0.5 mm ID, is not part of
the IC equipment: Inline Ultrafiltration 2 -
push mode.
a) 6.1826.390
Pump tubing LFL (yellow/yellow), 3 stoppers
b) 6.1826.320
Pump tubing LFL (orange/yellow), 3 stoppers

3.3 Components of the ultrafiltration cell ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
8￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration 2 - push mode
3.3 Components of the ultrafiltration cell
1 2 3 4 1
Figure 3 Ultrafiltration cell – Parts
1Stopper 2Sample chamber
3Filtrate chamber 4Screws
for screwing together the sample chamber
and the filtrate chamber

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Overview
IC equipment: Inline Ultrafiltration 2 - push mode ￭￭￭￭￭￭￭￭ 9
3.4 Connectors of the ultrafiltration cell
1 2 3 4 5
Figure 4 Ultrafiltration cell – Connectors
1Filtrate chamber 2Filtrate outlet
labeled OUT
3Sample chamber 4Sample inlet
labeled IN
5Sample outlet
labeled OUT

4.1 Preparing the ultrafiltration cell ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
10 ￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration 2 - push mode
4 Installation
4.1 Preparing the ultrafiltration cell
Inserting the filtration membrane
Accessories ￭Ultrafiltration cell 2 (6.02729.110)
￭Filtration membrane 1 (6.2714.020)
￭4 mm hex key (6.2621.030)
￭Plastic tweezers (6.2831.010)
1Removing the stoppers
￭Remove the 3 green stoppers by hand.
￭Place the ultrafiltration cell on the table with the filtrate chamber
facing upwards. The screws face upwards.
2Removing the screws
￭Loosen the screws with the hex key.
The screws cannot be removed from the filtrate chamber.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation
IC equipment: Inline Ultrafiltration 2 - push mode ￭￭￭￭￭￭￭￭ 11
3Disassembling the ultrafiltration cell
Remove the filtrate chamber of the ultrafiltration cell.
4Cleaning the ultrafiltration cell
CAUTION
Damage to the ultrafiltration cell
Organic solvents (e.g. acetone) corrode and damage the ultrafiltra-
tion cell material (PMMA).
Use ultrapure water or a water-ethanol mixture (70:30) for clean-
ing the ultrafiltration cell.
￭Rinse both chambers with ultrapure water.
￭Dry both chambers with a lint-free cloth.

4.1 Preparing the ultrafiltration cell ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
12 ￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration 2 - push mode
5Wetting the filtration membrane
NOTICE
The package of the filtration membranes contains sheets of differ-
ent thicknesses and colors:
￭The thin white sheets are the filtration membranes. Only insert
filtration membranes into the ultrafiltration cell.
￭The firm white cardboard is a cover. The cover protects the fil-
tration membranes. Do not insert the cover into the ultrafiltra-
tion cell.
￭The thin light-blue sheets are separation sheets. The separation
sheets are placed between 2 filtration membranes. Do not
insert the separation sheets into the ultrafiltration cell.
￭Using the tweezers, take a filtration membrane out of the pack-
age.
￭Place the filtration membrane in a petri dish filled with ultrapure
water and allow to hydrate for approx. 2 minutes.
6Inserting the filtration membrane
NOTICE
The filtration membrane must not dry out before it is inserted!