Metrohm 815 User manual

815 Robotic Soliprep for LC
2.815.4110
Manual
8.815.8002EN / 2020-02-29

Metrohm AG
CH-9100 Herisau
Switzerland
Phone [phone]
Fax [phone]
[email protected]
www.metrohm.com
815 Robotic Soliprep for LC
2.815.4110
Manual
8.815.8002EN / 2020-02-29

Technical Communication
Metrohm AG
CH-9100 Herisau
[email protected]
This documentation is protected by copyright. All rights reserved.
This documentation has been prepared with great care. However, errors
can never be entirely ruled out. Please send comments regarding possible
errors to the address above.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents
815 Robotic Soliprep for LC ￭￭￭￭￭￭￭￭ III
Table of contents
1 Introduction 1
1.1 Instrument description ......................................................... 1
1.2 Intended use ......................................................................... 2
1.3 About the documentation ................................................... 2
1.3.1 Symbols and conventions ........................................................ 2
1.4 Safety instructions ................................................................ 3
1.4.1 General notes on safety ........................................................... 3
1.4.2 Electrical safety ........................................................................ 3
1.4.3 Personnel safety ....................................................................... 4
1.5 Recycling and disposal ......................................................... 5
2 Installation 6
2.1 Preparing the Sample Processor ......................................... 6
2.2 Connecting the instrument to the power grid ................... 8
2.3 Installing MagIC Net™ ......................................................... 9
2.4 Configuring the Swing Heads .............................................. 9
2.5 Mounting the Swing Heads ............................................... 13
2.6 Mounting the deflector, Luer connector and collection
container ............................................................................. 16
2.7 Mounting the transfer robotic arm ................................... 20
2.8 Mounting the Polytron robotic arm .................................. 24
2.9 Washing station and drip pan ........................................... 25
2.10 Connecting and setting up the peristaltic pump ............. 27
2.11 Installing the rinsing tubing .............................................. 29
2.12 Placing tubing and cables in the guide chain .................. 29
2.13 Assembling and mounting the Polytron® drive ................ 31
2.14 Mounting the rinsing tubings for the Polytron® ............. 31
2.15 Connecting the Polytron® .................................................. 32
2.16 Mounting the safety shield ................................................ 33
2.17 Installing the Dosino .......................................................... 34
2.18 Equipping the rack ............................................................. 37
3 Defining rack positions 39
4 Adjusting rack and robotic arm 41

Table of contents ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
IV ￭￭￭￭￭￭￭￭ 815 Robotic Soliprep for LC
5 Setting lift positions 45
5.1 Lift position for sample beakers ....................................... 45
5.2 Lift position for picking up needles and filters ................ 46
5.3 Lift positions for special beakers ...................................... 47
5.4 Lift positions for stripping needles and filters ................. 49
5.5 Lift position for the Luer connector .................................. 51
5.6 Lift position for the Polytron ............................................. 52
5.7 Lift position for the washing station ................................ 53
6 Controlling the Polytron® 55
7 Accessories 60
Index 61

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
815 Robotic Soliprep for LC ￭￭￭￭￭￭￭￭ 1
1 Introduction
1.1 Instrument description
The 815 Robotic Soliprep systems are automation systems suitable for a
variety of uses. They have been designed exclusively for utilization in facto-
ries and laboratories and cover a wide range of applications. Soliprep sys-
tems can be used for automation purposes wherever a complex sample
preparation of solid substances is required, e.g. for pharmaceutical or food
analysis.
The Robotic Sample Processors, which have proven their worth for many
years, form the basis for a versatile system that has been expanded to
include opportunities for the dispersion, filtration and bottling of samples
of all sorts. This versatile concept can be expanded as required.
Thanks to the integration of high-performance communication interfaces
(USB, RS-232), they can be incorporated seamlessly into a variety of instru-
ment systems, and not only those manufactured by Metrohm. Operation
by the high performance tiamo™ or MagIC Net™ software ensures not
only user-friendly operation and programming of automation sequences,
but also allows 100% conformity for the entire automation system with
FDA (Food and Drug Administration) regulations. This applies in particular
with regard to regulation 21 CFR Part 11, Electronic records and signa-
tures.
815 Robotic Soliprep for Liquid Chromatography
￭Dispersion, filtration, dilution and sample transfer
The universal automation system for sample preparation in instrumental
chromatography. A workstation equipped with a dispersion device is used
to shred the sample. The dispersion aggregate is cleaned in a special rins-
ing station. The second workstation is equipped for sample transfer with a
universal robotic arm with a Luer adapter. It is used for picking up syringe
needles (for aspirating the dispersed sample and bottling the filtrate in
sealed vials) and membrane filters (for filtering and injecting the sample in
a capillary).
Needles and filters are charged in replaceable inserts. The needles and fil-
ters are automatically stripped off after use and disposed of in a waste
container.
Two versatile dosing drives (800 Dosino) are used as an auxiliary device for
diluting the sample and for transferring the sample solution.

1.2 Intended use ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
2￭￭￭￭￭￭￭￭ 815 Robotic Soliprep for LC
1.2 Intended use
The 815 Robotic Soliprep for LC is designed for the automatic analysis of
larger-sized sample series in analytical laboratories. The functioning of the
automation system comprises crushing, homogenizing and dissolving of
solid samples as well as filtering and transferring the resulting sample solu-
tions. Its main area of application is the sample preparation in instrumen-
tal chromatography. It is not suitable for usage in biochemical, biological
or medical environments in its basic equipment version.
This instrument is suitable for processing chemicals and flammable sam-
ples. Therefore, the use of the 815 Robotic Soliprep for LC requires the
user to have basic knowledge and experience in handling toxic and caustic
substances. Knowledge regarding the application of fire prevention meas-
ures prescribed for laboratories is also mandatory.
1.3 About the documentation
1.3.1 Symbols and conventions
The following symbols and formatting may appear in this documentation:
Cross-reference to figure legend
The first number refers to the figure number, the sec-
ond to the instrument part in the figure.
Instruction step
Carry out these steps in the sequence shown.
Method Dialog text, parameter in the software
File ▶ New Menu or menu item
[Next] Button or key
WARNING
This symbol draws attention to a possible life-threat-
ening hazard or risk of injury.
WARNING
This symbol draws attention to a possible hazard due
to electrical current.
WARNING
This symbol draws attention to a possible hazard due
to heat or hot instrument parts.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
815 Robotic Soliprep for LC ￭￭￭￭￭￭￭￭ 3
WARNING
This symbol draws attention to a possible biological
hazard.
CAUTION
This symbol draws attention to possible damage to
instruments or instrument parts.
NOTE
This symbol highlights additional information and
tips.
1.4 Safety instructions
1.4.1 General notes on safety
WARNING
Operate this instrument only according to the information contained in
this documentation.
This instrument left the factory in a flawless state in terms of technical
safety. To maintain this state and ensure non-hazardous operation of the
instrument, the following instructions must be observed carefully.
1.4.2 Electrical safety
The electrical safety when working with the instrument is ensured as part
of the international standard IEC 61010.
WARNING
Only personnel qualified by Metrohm are authorized to carry out service
work on electronic components.
WARNING
Never open the housing of the instrument. The instrument could be
damaged by this. There is also a risk of serious injury if live components
are touched.
There are no parts inside the housing which can be serviced or replaced
by the user.

1.4 Safety instructions ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
4￭￭￭￭￭￭￭￭ 815 Robotic Soliprep for LC
Supply voltage
WARNING
An incorrect supply voltage can damage the instrument.
Only operate this instrument with a supply voltage specified for it (see
rear panel of the instrument).
Protection against electrostatic charges
WARNING
Electronic components are sensitive to electrostatic charges and can be
destroyed by discharges.
Do not fail to pull the power cord out of the power socket before you
set up or disconnect electrical plug connections at the rear of the
instrument.
1.4.3 Personnel safety
WARNING
Personnel are not permitted to reach into the working area of a Sample
Processor while operations are running!
A considerable risk of injury exists for users who undertake manual
interventions to the automatic running of the instrument.
The various drives in a Sample Processor (including Swing Heads) have
considerable power in order to ensure that they are able to be operated
perfectly, even with greater loads. They are equipped with an electronic
overload protection that is activated in the presence of excessive mechani-
cal resistance.
WARNING
The Sample Processor does not provide sufficient protection during the
processing of potentially infectious samples or reagents.
Implement appropriate protective measures.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
815 Robotic Soliprep for LC ￭￭￭￭￭￭￭￭ 5
1.5 Recycling and disposal
This product is covered by European Directive 2012/19/EU, WEEE – Waste
Electrical and Electronic Equipment.
The correct disposal of your old instrument will help to prevent negative
effects on the environment and public health.
More details about the disposal of your old instrument can be obtained
from your local authorities, from waste disposal companies or from your
local dealer.

2.1 Preparing the Sample Processor ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
6￭￭￭￭￭￭￭￭ 815 Robotic Soliprep for LC
2 Installation
These installation instructions assume that the required adjustment of
turntable and Swing Heads was already performed for the concerned
Robotic Sample Processor.
2.1 Preparing the Sample Processor
Connecting the Swing Heads
NOTICE
The necessary settings must first be made in the (MagIC Net) control
software before the robotic arms can be mounted on the Swing Heads.
For this purpose, the two Swing Heads are connected but the robotic
arms are not yet mounted.
1Connect the Swing Head with reinforcement to tower 1 (on the
right), the Swing Head without reinforcement to tower 2 (on the
left).
The connector is located in each case on the rear side of a tower.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation
815 Robotic Soliprep for LC ￭￭￭￭￭￭￭￭ 7
The Swing Heads can be laid down flatly, but not with the drive disk
downwards.
Connecting the controller cable
1Connect the controller cable 6.2151.000 to the rear of the instru-
ment.
NOTICE
The plug on the instrument end of the 6.2151.000 controller cable is
protected against accidental disconnection by means of a pull-out pro-
tection feature. If you wish to pull out the plug, you first need to pull
back the outer plug sleeve marked with arrows.
Attaching the sample rack
1Attach the sample rack in such a way that both openings in the bot-
tom of the rack engage in the guide bolts of the turntable.

2.2 Connecting the instrument to the power grid ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
8￭￭￭￭￭￭￭￭ 815 Robotic Soliprep for LC
2Screw tight the handles of the sample rack.
2.2 Connecting the instrument to the power grid
WARNING
Electric shock from electrical potential
Risk of injury by touching live components or through moisture on live
parts.
￭Never open the housing of the instrument while the power cord is
still connected.
￭Protect live parts (e.g. power supply unit, power cord, connection
sockets) against moisture.
￭Unplug the power plug immediately if you suspect that moisture has
gotten inside the instrument.
￭Only personnel who have been issued Metrohm qualifications may
perform service and repair work on electrical and electronic parts.
Connecting the power cord
Accessories Power cord with the following specifications:
￭Length: max. 2 m
￭Number of cores: 3, with protective conductor
￭Instrument plug: IEC 60320 type C13
￭Conductor cross-section 3x min. 0.75 mm2 / 18 AWG
￭Power plug:
– according to customer requirement (6.2122.XX0)
– min. 10 A
NOTICE
Do not use a not permitted power cord!
1Plugging in the power cord
￭Plug the power cord into the instrument's power socket.
￭Connect the power cord to the power grid.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation
815 Robotic Soliprep for LC ￭￭￭￭￭￭￭￭ 9
2.3 Installing MagIC Net™
Installing MagIC Net
1Insert the MagIC Net CD into the CD drive of the PC and carry out
the installation following the instructions. After this, start MagIC Net.
2.4 Configuring the Swing Heads
Connecting the controller cable on the computer
1Connect the controller cable to a USB connector of the computer.

2.4 Configuring the Swing Heads ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
10 ￭￭￭￭￭￭￭￭ 815 Robotic Soliprep for LC
For Windows 2000: The instrument is recognized and the driver is
installed automatically.
For Windows XP: The instrument is recognized and the installation
assistant for the driver is started automatically. Select the option
"Install software automatically" and click on [Continue]. Exit the
assistant with [Finish].
For Windows Vista: the instrument is recognized and the installa-
tion wizard for the driver is started automatically. Select the option
"Find and install driver software". Agree to all of the requests that
follow. The installation assistant will be exited automatically.
Instrument registration
The USB Sample Processor is automatically recognized by MagIC Net.
1Confirm with [Yes].
The properties window for configuring the instrument is displayed.
2Confirm with [OK].

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation
815 Robotic Soliprep for LC ￭￭￭￭￭￭￭￭ 11
Configuring the Swing Heads
Configure the robotic arms for both towers.
1Click the Configuration icon.
2Double-click on the instrument name 815 Robotic USB … in the
Devices window.
3First click on the Tower 1 tab and then on Configuration.
Enter the following settings:
￭Swing offset –8.6°
￭Maximum swing range 122°
￭Swing radius 149.8 mm
￭Rotation offset 0.0°

2.4 Configuring the Swing Heads ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
12 ￭￭￭￭￭￭￭￭ 815 Robotic Soliprep for LC
￭Swing direction –
4First click on the Tower 2 tab and then on Configuration.
Enter the following settings:
￭Swing offset 0.0°
￭Maximum swing range 115.5°
￭Swing radius 110 mm
￭Rotation offset 0.0°
￭Swing direction +
5Finish the settings with [OK].
For the settings to take effect, the instrument must be reinitialized.
6Click on [OK].
7Attach a sample rack on the turntable of the instrument.
8Right-click on the instrument name 815 Robotic USB ... in the
Devices window and click on Initialize.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Installation
815 Robotic Soliprep for LC ￭￭￭￭￭￭￭￭ 13
The Sample Processor is initialized. The settings of the Swing Heads
and robotic arms are now activated.
2.5 Mounting the Swing Heads
Preparing the towers
Both lifts must be moved to a suitable position for the mounting of the
Swing Head and robotic arms to be comfortable.
1Opening the manual control
In the symbol bar of MagIC Net, click on the hand symbol.
2Under Device selection, first select All devices and then the 815
Robotic USB .... Click the Tower 1 tab.
3Moving the lift to position
Under Rack position, click on [Start].
The sample rack moves to a defined position. Only now, the lift can
be moved.

2.5 Mounting the Swing Heads ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
14 ￭￭￭￭￭￭￭￭ 815 Robotic Soliprep for LC
4Under Lift position, click on the arrow button "down" until the lift
1 has moved approx. 180 mm downwards.
Both lifts are now in a suitable position so that all screws for mount-
ing the Swing Heads can be reached easily.
Dismounting the titration head holder
1
1
2
1Unscrew the screws on the outside and inside of the holder on tower
2 (left).
2Unscrew and remove the holder from the holder plate of the guide
chain.
3Remove the holder also on tower 1.
Use the supplied hex key. Set the screws aside for later use.