Metrohm 850 Professional IC User manual

850 Professional IC
Anion – MSM-HC – MCS – LP Gradient – 2.850.2230
Manual
8.850.8026EN

Metrohm AG
CH-9101 Herisau
Switzerland
Phone [phone]
Fax [phone]
[email protected]
www.metrohm.com
850 Professional IC
Anion – MSM-HC – MCS – LP Gradi-
ent – 2.850.2230
Manual
8.850.8026EN 09.2009 zst

Teachware
Metrohm AG
CH-9101 Herisau
[email protected]
This documentation is protected by copyright. All rights reserved.
Although all the information given in this documentation has been
checked with great care, errors cannot be entirely excluded. Should you
notice any mistakes please send us your comments using the address
given above.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents
850 Professional IC–Anion – MSM-HC – MCS – LP Gradient ￭￭￭￭￭￭￭￭ III
Table of contents
1 Introduction 1
1.1 Instrument description ......................................................... 1
1.2 Intended use ......................................................................... 3
1.3 About the documentation ................................................... 4
1.3.1 Symbols and conventions ........................................................ 4
1.4 Safety instructions ................................................................ 5
1.4.1 General notes on safety ........................................................... 5
1.4.2 Electrical safety ........................................................................ 5
1.4.3 Tubing and capillary connections ............................................. 6
1.4.4 Flammable solvents and chemicals ........................................... 6
1.4.5 Recycling and disposal ............................................................. 6
2 Overview of the instrument 8
2.1 Front ...................................................................................... 8
2.2 Rear ...................................................................................... 10
3 Installation 13
3.1 About this chapter .............................................................. 13
3.2 Initial installation ................................................................ 13
3.3 Installation of the low pressure gradient ......................... 16
3.4 Installation diagram .......................................................... 17
3.5 Setting up the instrument .................................................. 20
3.5.1 Packaging .............................................................................. 20
3.5.2 Checks .................................................................................. 20
3.5.3 Location ................................................................................ 20
3.6 Capillary connections in the IC system ............................. 20
3.7 Rear of the instrument ....................................................... 22
3.7.1 Rollers and handle ................................................................. 22
3.7.2 Positioning and connecting the detector ................................ 25
3.7.3 Transport locking screws ....................................................... 27
3.7.4 Leak sensor ........................................................................... 27
3.7.5 Drainage tubing ..................................................................... 28
3.8 Capillary and cable feed-throughs .................................... 30
3.9 Eluent ................................................................................... 33
3.9.1 Connecting eluent bottle ....................................................... 33
3.10 Eluent degasser ................................................................. 37
3.11 Low pressure gradient ....................................................... 38

Table of contents ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
IV ￭￭￭￭￭￭￭￭ 850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
3.12 High pressure pump ........................................................... 42
3.12.1 Capillary connections high pressure pump/purge valve ........... 42
3.12.2 Connecting low pressure gradient ......................................... 43
3.12.3 Deaerating the high pressure pump ....................................... 44
3.13 Inline filter ........................................................................... 46
3.14 Pulsation damper ............................................................... 47
3.15 Sample degasser ................................................................. 48
3.16 Injection valve ..................................................................... 50
3.16.1 Connecting the injection valve ............................................... 50
3.16.2 Mode of operation of the injection valve ............................... 51
3.16.3 Selecting the sample loop ...................................................... 52
3.17 Column thermostat ............................................................ 52
3.18 High Capacity Metrohm Suppressor Module
(MSM-HC) ............................................................................ 55
3.18.1 General information on the MSM-HC .................................... 55
3.18.2 Connecting the MSM-HC ....................................................... 55
3.19 Peristaltic pump .................................................................. 58
3.19.1 Principle of the peristaltic pump ............................................. 58
3.19.2 Installing the peristaltic pump ................................................ 59
3.20 Metrohm CO2 suppressor (MCS) ....................................... 63
3.20.1 General information on the MCS ........................................... 63
3.20.2 Connecting MCS ................................................................... 63
3.20.3 Installing the adsorption cartridges ........................................ 64
3.21 Conductivity detector ......................................................... 66
3.22 Connecting the instrument ................................................ 69
3.22.1 Connecting the instrument to the PC ..................................... 69
3.22.2 Connecting the instrument to mains supply ........................... 69
3.23 Guard column ..................................................................... 70
3.24 Separation column ............................................................. 72
4 Start-up 74
4.1 Initial start-up ..................................................................... 74
4.2 Conditioning ........................................................................ 75
5 Operation and maintenance 77
5.1 General information ........................................................... 77
5.1.1 Care ...................................................................................... 77
5.1.2 Maintenance by Metrohm Service .......................................... 77
5.1.3 Operation .............................................................................. 78
5.1.4 Shutting down ...................................................................... 78
5.2 Capillary connections ......................................................... 78
5.2.1 Operation .............................................................................. 78

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents
850 Professional IC–Anion – MSM-HC – MCS – LP Gradient ￭￭￭￭￭￭￭￭ V
5.3 Door ..................................................................................... 79
5.4 Eluent ................................................................................... 79
5.4.1 Production ............................................................................. 79
5.4.2 Operation .............................................................................. 80
5.5 High pressure pump ........................................................... 80
5.5.1 Protection .............................................................................. 80
5.5.2 Maintenance ......................................................................... 81
5.6 Inline filter ........................................................................... 91
5.6.1 Maintenance ......................................................................... 91
5.7 Inline sample preparation .................................................. 93
5.8 Rinsing the sample path .................................................... 93
5.9 Sample degasser ................................................................. 95
5.9.1 Operation .............................................................................. 95
5.10 Injection valve .................................................................... 95
5.10.1 Protection .............................................................................. 95
5.11 High Capacity Metrohm Suppressor Module
(MSM-HC) ............................................................................ 95
5.11.1 Protection .............................................................................. 95
5.11.2 Operation .............................................................................. 95
5.11.3 Maintenance ......................................................................... 96
5.12 Peristaltic pump ................................................................ 101
5.12.1 Operation ............................................................................ 101
5.12.2 Maintenance ....................................................................... 102
5.13 Metrohm CO2 suppressor (MCS) ..................................... 104
5.13.1 Replacing the CO2 adsorption cartridge ............................... 104
5.13.2 Regenerating the H2O adsorption cartridge .......................... 104
5.14 Conductivity detector ....................................................... 105
5.14.1 Maintenance ....................................................................... 105
5.15 Separation column ........................................................... 105
5.15.1 Separating efficiency ............................................................ 105
5.15.2 Protection ............................................................................ 106
5.15.3 Storage ............................................................................... 106
5.15.4 Regeneration ....................................................................... 106
5.16 Quality Management and validation with Metrohm .... 107
6 Troubleshooting 108
6.1 Problems and their solutions .......................................... 108
7 Technical specifications 113
7.1 Reference conditions ........................................................ 113
7.2 Instrument ......................................................................... 113
7.3 Leak sensor ....................................................................... 113

Table of contents ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
VI ￭￭￭￭￭￭￭￭ 850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
7.4 Ambient conditions .......................................................... 113
7.5 Housing ............................................................................. 114
7.6 Eluent degasser ................................................................ 114
7.7 Low pressure gradient ..................................................... 114
7.8 High pressure pump ......................................................... 115
7.9 Sample degasser ............................................................... 116
7.10 Injection valve ................................................................... 116
7.11 Column thermostat .......................................................... 116
7.12 High Capacity Metrohm Suppressor Module
(MSM-HC) .......................................................................... 117
7.13 Peristaltic pump ................................................................ 117
7.14 Metrohm CO2 suppressor (MCS) ..................................... 117
7.15 Conductivity measuring system ...................................... 118
7.16 Mains connection ............................................................. 119
7.17 Interfaces .......................................................................... 119
7.18 Safety specification .......................................................... 120
7.19 Electromagnetic compatibility (EMC) ............................. 120
7.20 Weight ............................................................................... 120
8 Conformity and warranty 121
8.1 Declaration of Conformity ............................................... 121
8.2 Quality Management Principles ...................................... 122
8.3 Warranty (guarantee) ....................................................... 123
9 Accessories 125
9.1 Scope of delivery .............................................................. 125
9.2 Optional accessories ........................................................ 136
Index 138

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures
850 Professional IC–Anion – MSM-HC – MCS – LP Gradient ￭￭￭￭￭￭￭￭ VII
Table of figures
Figure 1 Front 850 Professional IC – Anion – MSM-HC – MCS – LP Gradi-
ent .................................................................................................... 8
Figure 2 Rear 850 Professional IC Anion – MSM-HC – MCS – LP Gradient ..... 10
Figure 3 Installation diagram ......................................................................... 18
Figure 4 Connection of capillaries with pressure screws ................................ 21
Figure 5 Rollers and handle ........................................................................... 23
Figure 6 Handle as MPak holder .................................................................... 24
Figure 7 Removable rear panel ...................................................................... 25
Figure 8 Connection for the leak sensor on the rear of the instrument .......... 28
Figure 9 Drainage tubing .............................................................................. 29
Figure 10 Capillary feed-throughs on the doors ............................................... 31
Figure 11 Capillary feed-throughs base tray/covering plate .............................. 32
Figure 12 Installing eluent bottle attachment .................................................. 34
Figure 13 Mounting aspiration filter ................................................................ 34
Figure 14 Installing tubing weighting and aspiration filter ............................... 35
Figure 15 Eluent aspiration tubing fully equipped. ........................................... 35
Figure 16 Eluent bottle – connected ............................................................... 36
Figure 17 Eluent degasser ............................................................................... 37
Figure 18 Low pressure gradient ..................................................................... 39
Figure 19 Connecting mixing coil for low pressure gradients ........................... 40
Figure 20 Connecting eluent connection tubings ............................................ 41
Figure 21 Capillary connections high pressure pump/purge valve .................... 42
Figure 22 Mixing coil for low pressure gradients connection ........................... 44
Figure 23 Deaerating the high pressure pump ................................................. 45
Figure 24 Connecting inline filter .................................................................... 47
Figure 25 Pulsation damper – Connection ....................................................... 48
Figure 26 Sample degasser ............................................................................. 49
Figure 27 Injection valve – connected ............................................................. 50
Figure 28 Injection valve – Positions ................................................................ 51
Figure 29 Column thermostat ......................................................................... 53
Figure 30 MSM-HC – Connectors .................................................................... 56
Figure 31 Peristaltic pump ............................................................................... 58
Figure 32 Installing the pump tubing .............................................................. 59
Figure 33 Install pump tubing connection with filter ....................................... 60
Figure 34 Install pump tubing connection without filter .................................. 61
Figure 35 MCS – connection ........................................................................... 63
Figure 36 Adsorption cartridge holder ............................................................. 65
Figure 37 Conductivity detector front .............................................................. 67
Figure 38 Conductivity detector rear ............................................................... 68
Figure 39 Connection detector – MCS ............................................................ 69
Figure 40 Removing piston ............................................................................. 82
Figure 41 Components of the piston cartridge ................................................ 83
Figure 42 Tool for piston seal 6.2617.010 ....................................................... 84
Figure 43 Removing the piston seal ................................................................. 85
Figure 44 Inserting the piston seal into the tool ............................................... 85

Table of figures ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
VIII ￭￭￭￭￭￭￭￭ 850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
Figure 45 Inserting the piston seal into the pump head ................................... 86
Figure 46 Removing valves .............................................................................. 87
Figure 47 Dismantling valve ............................................................................ 88
Figure 48 Components of the inlet valve and outlet valve ................................ 89
Figure 49 Changing the filter .......................................................................... 91
Figure 50 MSM-HC – Components ................................................................. 98
Figure 51 Pump tubing connection – Changing the filter ............................... 103

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850 Professional IC–Anion – MSM-HC – MCS – LP Gradient ￭￭￭￭￭￭￭￭ 1
1 Introduction
1.1 Instrument description
The instrument 850 Professional IC – Anion – MSM-HC – MCS – LP
Gradient (2.850.2230) is one of the model versions of the Professional IC
line of instruments manufactured by the Metrohm Company. The Profes-
sional IC line of instruments is distinguished by:
￭the intelligence of its components, which are able to monitor and
optimize all functions and to provide documentation according to FDA
requirements.
￭its compactness.
￭its flexibility. A suitable model version exists for every application.
Individual instruments can be converted, expanded or modified to cre-
ate a different instrument as needed.
￭its transparency. All components are easily accessible and arranged in
a clear manner.
￭its safety. Chemicals and electronics are separated and a leak sensor is
integrated in the wet end.
￭its environmental compatibility.
￭low noise emission.
The instrument is operated with MagIC Net software. It is connected via
a USB connection to a PC on which MagIC Net is installed. The software
automatically recognizes the instrument and checks its functional readi-
ness. MagIC Net controls and monitors the instrument, evaluates the mea-
sured data and administers it in a database. The operation of MagIC Net is
described in the online help or in the tutorial for MagIC Net.
The low pressure mixing valve and two additional eluent degassers in the
lower area of the instrument are used for the controlled mixing of up to 3
eluents.
The instrument contains the following components:
Eluent degasser
The eluent degasser removes gas bubbles and dissolved gases from the
eluent. For degassing, the eluent flows into a vacuum chamber through a
special fluoropolymer capillary.
Low pressure gradient
Up to three different eluents can be mixed with the low pressure gradient.
The mixing takes place in the low pressure zone, i.e. before the eluent is
conveyed to the high pressure pump.

1.1 Instrument description ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
2￭￭￭￭￭￭￭￭ 850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
High pressure pump
The intelligent and low pulsation high pressure pump pumps the eluent
through the system. It is equipped with a chip on which its technical spec-
ifications and "life history" (operating hours, service data, ... ) are saved.
Inline filter
Inline filters protect the separation column securely against possible con-
tamination from the eluent. Inline filters can however also just as well be
used for the purpose of protecting other sensitive components against
contaminations in the solutions used. The fine 2 µm material of the readily
and easily replaceable filter platelets removes particles such as bacteria
and algae from the solutions.
Pulsation damper
The pulsation damper protects the separation column from damage
caused by pressure fluctuations when switching the injection valve, and
reduces interfering pulsations during highly sensitive measurements.
Sample degasser
The sample degasser removes gas bubbles and disolved gases from the
sample. For degassing, the sample flows into a vacuum chamber through
a special fluoropolymer capillary.
Injection valve
The injection valve connects the eluent and sample path through rapid
and precise valve switchover. A precisely measured amount of sample
solution is injected and rinsed with eluent onto the separation column.
Column thermostat
The column thermostat controls the temperature of the column and elu-
ent channel and thus ensures stable measuring conditions. It provides
space for 2 separation columns.
High Capacity Metrohm Suppressor Module (MSM-HC)
The MSM-HC is used for chemical suppression with high capacity during
anion analysis. It is pressure-stable, robust and resistant to solvents.
Peristaltic pump
The Peristaltic pump is used for pumping sample and auxiliary solutions. It
can rotate in both directions.
Metrohm CO2 suppressor (MCS)
The MCS removes the CO2 from the eluent flow. This reduces the back-
ground conductivity, improves the detection sensitivity and minimizes the
injection and carbonate peaks.

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850 Professional IC–Anion – MSM-HC – MCS – LP Gradient ￭￭￭￭￭￭￭￭ 3
Conductivity detector
The conductivity detector continuously measures the conductivity of the
liquid passing through and indicates these signals in digital form (DSP –
Digital Signal Processing). The conductivity detector exhibits outstanding
thermal stability and thus guarantees reproducible measuring conditions.
Separation column
The intelligent separation column is the heart of the ion chromatographic
analysis. It separates the different components corresponding to their
interactions with the column. The Metrohm analytical columns are equip-
ped with a chip on which their technical specifications and their history
(first use / setting up, operating hours, injections, ... ) are saved.
1.2 Intended use
The 850 Professional IC – Anion – MSM-HC – MCS – LP Gradient
can be used for the ion chromatographic determination of anions with
sequential suppression in cases where the complexity of the separation
problem requires the use of gradients.
Sequential suppression consists of:
￭Chemical suppression by the Metrohm Suppressor Module (MSM) and
subsequent
￭CO2 suppression by the Metrohm CO2 Suppressor (MCS) (see Chapter
3.20, page 63).
The use of this technology reduces background conductivity to an abso-
lute minimum.
If required, the instrument can also be used as a normal isocratic anion
system with sequential suppression or for the determination of cations
and anions without suppression.
The present instrument is suitable for processing chemicals and flammable
samples. The usage of the 850 Professional IC – Anion – MSM-HC – MCS
– LP Gradient therefore requires that the user has basic knowledge and
experience in the handling of toxic and caustic substances. Knowledge
with respect to the application of the fire prevention measures prescribed
for laboratories is also mandatory.

1.3 About the documentation ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
4￭￭￭￭￭￭￭￭ 850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
1.3 About the documentation
CAUTION
Please read through this documentation carefully before putting the
instrument into operation. The documentation contains information
and warnings which have to be followed by the user in order to ensure
safe operation of the instrument.
1.3.1 Symbols and conventions
The following symbols and styles are used in this documentation:
Cross-reference to figure legend
The first number refers to the figure number, the
second to the instrument part in the figure.
Instruction step
Carry out these steps in the sequence shown.
Warning
This symbol draws attention to a possible life hazard
or risk of injury.
Warning
This symbol draws attention to a possible hazard due
to electrical current.
Warning
This symbol draws attention to a possible hazard due
to heat or hot instrument parts.
Warning
This symbol draws attention to a possible biological
hazard.
Caution
This symbol draws attention to a possible damage of
instruments or instrument parts.
Note
This symbol marks additional information and tips.

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850 Professional IC–Anion – MSM-HC – MCS – LP Gradient ￭￭￭￭￭￭￭￭ 5
1.4 Safety instructions
1.4.1 General notes on safety
WARNING
This instrument may only be operated in accordance with the specifica-
tions in this documentation.
This instrument has left the factory in a flawless state in terms of technical
safety. To maintain this state and ensure non-hazardous operation of the
instrument, the following instructions must be observed carefully.
1.4.2 Electrical safety
The electrical safety when working with the instrument is ensured as part
of the international standard IEC 61010.
WARNING
Only personnel qualified by Metrohm are authorized to carry out service
work on electronic components.
WARNING
Never open the housing of the instrument. The instrument could be
damaged by this. There is also a risk of serious injury if live components
are touched.
There are no parts inside the housing which can be serviced or replaced
by the user.
Mains voltage
WARNING
An incorrect mains voltage can damage the instrument.
Only operate this instrument with a mains voltage specified for it (see
rear panel of the instrument).

1.4 Safety instructions ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
6￭￭￭￭￭￭￭￭ 850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
Protection against electrostatic charges
WARNING
Electronic components are sensitive to electrostatic charges and can be
destroyed by discharges.
Always pull the mains cable out of the mains connection socket before
connecting or disconnecting electrical appliances on the rear panel of
the instrument.
1.4.3 Tubing and capillary connections
CAUTION
Leaks in tubing and capillary connections are a safety risk. Tighten all
connections well by hand. Avoid applying excessive force to tubing
connections. Damaged tubing ends lead to leakage. Appropriate tools
can be used to loosen connections.
Check the connections regularly for leakage. If the instrument is used
mainly in unattended operation, then weekly inspections are manda-
tory.
1.4.4 Flammable solvents and chemicals
WARNING
All relevant safety measures are to be observed when working with
flammable solvents and chemicals.
￭Set up the instrument in a well-ventilated location (e.g. laboratory
flue).
￭Keep all sources of flame far from the workplace.
￭Clean up spilled fluids and solids immediately.
￭Follow the safety instructions of the chemical manufacturer.
1.4.5 Recycling and disposal
This product is covered by European Directive 2002/96/EC, WEEE – Waste
from Electrical and Electronic Equipment.
The correct disposal of your old equipment will help to prevent negative
effects on the environment and public health.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
850 Professional IC–Anion – MSM-HC – MCS – LP Gradient ￭￭￭￭￭￭￭￭ 7
More details about the disposal of your old equipment can be obtained
from your local authorities, from waste disposal companies or from your
local dealer.

2.1 Front ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
8￭￭￭￭￭￭￭￭ 850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
2 Overview of the instrument
2.1 Front
9
1
2
3
5
4
6
7
8
10
11
12
13
14
15
16
17
Figure 1 Front 850 Professional IC – Anion – MSM-HC – MCS – LP Gradient
1Eluent degasser
See Chapter 3.10, Page 37.
2High pressure pump
See Chapter 3.12, Page 42.

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850 Professional IC–Anion – MSM-HC – MCS – LP Gradient ￭￭￭￭￭￭￭￭ 9
3Purge valve
For deaerating the high pressure pump See
Chapter 3.12.3, Page 44.
4Pulsation damper
See Chapter 3.14, Page 47.
5Injection valve
See Chapter 3.16, Page 50.
6Sample degasser
See Chapter 3.15, Page 48. Use optional.
7Column thermostat
See Chapter 3.17, Page 52.
8Column holder
For two separation columns (see Chapter
3.24, page 72) in the column thermostat
and one outside the column thermostat.
9Detector chamber
Space for the conductivity detector See
Chapter 3.21, Page 66.
10 Peristaltic pump
See Chapter 3.19, Page 58
11 MSM-HC
See Chapter 3.18, Page 55
12 MCS
See Chapter 3.20, Page 63
13 Mixing valve
See Chapter 3.11, Page 38
14 Eluent degasser
For two additional eluents.(see Chapter
3.10, page 37)
15 Eluent aspiration tubing 6.1834.080 16 Connection tubing eluent degasser –
high pressure pump 6.1834.090
17 Column inlet capillary 6.1831.150

2.2 Rear ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
10 ￭￭￭￭￭￭￭￭ 850 Professional IC–Anion – MSM-HC – MCS – LP Gradient
2.2 Rear
1
22
21
219
20
10
17
15
7
6
5
4
318
11
912 13
8
14
16
Figure 2 Rear 850 Professional IC Anion – MSM-HC – MCS – LP Gradient
1Knurled screws
For fastening the rear panel (2-22) and han-
dle (5-2).
2Vacuum connection
For connecting further degassing chambers
in extension modules.
Labeled with Vacuum.