Metrohm 944 User manual

944 Professional UV/VIS Detector Vario
2.944.0010
Manual
8.944.8001EN / 2019-09-27

Metrohm AG
CH-9100 Herisau
Switzerland
Phone [phone]
Fax [phone]
[email protected]
www.metrohm.com
944 Professional UV/VIS Detector
Vario
2.944.0010
Manual
8.944.8001EN / 2019-09-27

Technical Communication
Metrohm AG
CH-9100 Herisau
[email protected]
This documentation is protected by copyright. All rights reserved.
This documentation has been prepared with great care. However, errors
can never be entirely ruled out. Please send comments regarding possible
errors to the address above.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents
944 Professional UV/VIS Detector Vario (2.944.0010) ￭￭￭￭￭￭￭￭ III
Table of contents
1 Introduction 1
1.1 Instrument description ......................................................... 1
1.2 Intended use ......................................................................... 1
1.3 About the documentation ................................................... 1
1.3.1 Symbols and conventions ........................................................ 2
1.4 Safety instructions ................................................................ 3
1.4.1 General notes on safety ........................................................... 3
1.4.2 Electrical safety ........................................................................ 3
1.4.3 Tubing and capillary connections ............................................. 4
1.4.4 Flammable solvents and chemicals ........................................... 4
1.4.5 Recycling and disposal ............................................................. 4
2 Overview of the instrument 6
2.1 Front ...................................................................................... 6
2.2 Rear ........................................................................................ 7
3 Installation 8
3.1 Setting up the instrument .................................................... 8
3.1.1 Packaging ................................................................................ 8
3.1.2 Checks .................................................................................... 8
3.1.3 Location .................................................................................. 8
3.2 Setup configurations ............................................................ 8
3.3 Base tray and bottle holder ............................................... 10
3.3.1 Basic information on base tray and bottle holder ................... 10
3.3.2 Mounting base tray and bottle holder (optional) .................... 10
3.4 Installing the flow-through cell ......................................... 15
3.5 Connecting the flow-through cell ..................................... 17
3.6 Connecting the instrument ................................................ 19
3.6.1 Connecting the instrument to a computer ............................. 19
3.6.2 Connecting the instrument to the power grid ........................ 19
4 Start-up 21
5 Operation 23
6 Operation and maintenance 24
6.1 Care ...................................................................................... 24
6.2 Maintenance by Metrohm Service .................................... 24
6.3 Door ..................................................................................... 25

Table of contents ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
IV ￭￭￭￭￭￭￭￭ 944 Professional UV/VIS Detector Vario (2.944.0010)
6.4 Replacing the UV lamp ....................................................... 25
6.5 Replacing the VIS lamp ...................................................... 28
6.6 Adjusting the lamp settings .............................................. 28
6.7 Cleaning the flow-through cell .......................................... 29
7 Troubleshooting 33
7.1 Problems and their solutions ............................................. 33
8 Technical specifications 34
8.1 Reference conditions .......................................................... 34
8.2 UV/VIS detector .................................................................. 34
8.3 Lamps .................................................................................. 36
8.4 Ambient conditions ............................................................ 36
8.5 Housing ............................................................................... 36
8.6 Weight ................................................................................. 37
8.7 Power connection ............................................................... 37
8.8 Interfaces ............................................................................. 37
9 Accessories 38
Index 39

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures
944 Professional UV/VIS Detector Vario (2.944.0010) ￭￭￭￭￭￭￭￭ V
Table of figures
Figure 1 Front ................................................................................................. 6
Figure 2 Rear .................................................................................................. 7
Figure 3 Setup configurations ......................................................................... 9
Figure 4 Removing the base tray ................................................................... 11
Figure 5 Mounting the base tray ................................................................... 12
Figure 6 Removing the bottle holder ............................................................. 13
Figure 7 Mounting the bottle holder ............................................................. 14
Figure 8 Cell block ........................................................................................ 15
Figure 9 Connecting the detector input ......................................................... 17
Figure 10 Connecting the detector outlet ........................................................ 18
Figure 11 Intensity spectrum OK ..................................................................... 22
Figure 12 Lamp intensity too high ................................................................... 22
Figure 13 Operating hours counter ................................................................. 25
Figure 14 Lamp module .................................................................................. 25
Figure 15 Lamp module – without UV lamp .................................................... 27
Figure 16 Flow-through cell – parts ................................................................. 31

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
944 Professional UV/VIS Detector Vario (2.944.0010) ￭￭￭￭￭￭￭￭ 1
1 Introduction
1.1 Instrument description
The 944 Professional UV/VIS Detector Vario is an independent instru-
ment for the photometric determination of light-absorbing substances in
the UV/VIS range. It serves as UV/VIS detector within an ion chromatogra-
phy system.
The 944 Professional UV/VIS Detector Vario is operated with the
MagIC Net software. It is connected to a PC on which MagIC Net is
installed with a USB cable. The software detects the instrument automati-
cally and checks its functional readiness. MagIC Net controls and monitors
the instrument, evaluates the measured data and manages it in a data-
base.
Additional information on operating MagIC Net can be found in the docu-
ment "MagIC Net Tutorial" or in the software's online help.
1.2 Intended use
The 944 Professional UV/VIS Detector Vario is used as an independent
detector with various analysis instruments of the Metrohm line of instru-
ments.
This instrument is suitable for processing chemicals and flammable sam-
ples. Therefore, the use of the 944 Professional UV/VIS Detector Vario
requires the user to have basic knowledge and experience in handling
toxic and caustic substances. Knowledge regarding the application of fire
prevention measures prescribed for laboratories is also mandatory.
1.3 About the documentation
CAUTION
Read through this documentation carefully before putting the instru-
ment into operation. The documentation contains information and
warnings which the user must follow in order to ensure safe operation
of the instrument.

1.3 About the documentation ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
2￭￭￭￭￭￭￭￭ 944 Professional UV/VIS Detector Vario (2.944.0010)
1.3.1 Symbols and conventions
The following symbols and formatting may appear in this documentation:
Cross-reference to figure legend
The first number refers to the figure number, the sec-
ond to the instrument part in the figure.
Instruction step
Carry out these steps in the sequence shown.
Method Dialog text, parameter in the software
File ▶ New Menu or menu item
[Next] Button or key
WARNING
This symbol draws attention to a possible life-threat-
ening hazard or risk of injury.
WARNING
This symbol draws attention to a possible hazard due
to electrical current.
WARNING
This symbol draws attention to a possible hazard due
to heat or hot instrument parts.
WARNING
This symbol draws attention to a possible biological
hazard.
CAUTION
This symbol draws attention to possible damage to
instruments or instrument parts.
NOTE
This symbol highlights additional information and
tips.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
944 Professional UV/VIS Detector Vario (2.944.0010) ￭￭￭￭￭￭￭￭ 3
1.4 Safety instructions
1.4.1 General notes on safety
WARNING
Operate this instrument only according to the information contained in
this documentation.
This instrument left the factory in a flawless state in terms of technical
safety. To maintain this state and ensure non-hazardous operation of the
instrument, the following instructions must be observed carefully.
1.4.2 Electrical safety
The electrical safety when working with the instrument is ensured as part
of the international standard IEC 61010.
WARNING
Only personnel qualified by Metrohm are authorized to carry out service
work on electronic components.
WARNING
Never open the housing of the instrument. The instrument could be
damaged by this. There is also a risk of serious injury if live components
are touched.
There are no parts inside the housing which can be serviced or replaced
by the user.
Supply voltage
WARNING
An incorrect supply voltage can damage the instrument.
Only operate this instrument with a supply voltage specified for it (see
rear panel of the instrument).

1.4 Safety instructions ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
4￭￭￭￭￭￭￭￭ 944 Professional UV/VIS Detector Vario (2.944.0010)
Protection against electrostatic charges
WARNING
Electronic components are sensitive to electrostatic charges and can be
destroyed by discharges.
Do not fail to pull the power cord out of the power socket before you
set up or disconnect electrical plug connections at the rear of the
instrument.
1.4.3 Tubing and capillary connections
CAUTION
Leaks in tubing and capillary connections are a safety risk. Tighten all
connections well by hand. Avoid applying excessive force to tubing con-
nections. Damaged tubing ends lead to leakage. Appropriate tools can
be used to loosen connections.
Check the connections regularly for leakage. If the instrument is used
mainly in unattended operation, then weekly inspections are manda-
tory.
1.4.4 Flammable solvents and chemicals
WARNING
All relevant safety measures are to be observed when working with
flammable solvents and chemicals.
￭Set up the instrument in a well-ventilated location (e.g. fume cup-
board).
￭Keep all sources of flame far from the workplace.
￭Clean up spilled liquids and solids immediately.
￭Follow the safety instructions of the chemical manufacturer.
1.4.5 Recycling and disposal
This product is covered by European Directive 2012/19/EU, WEEE – Waste
Electrical and Electronic Equipment.
The correct disposal of your old instrument will help to prevent negative
effects on the environment and public health.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
944 Professional UV/VIS Detector Vario (2.944.0010) ￭￭￭￭￭￭￭￭ 5
More details about the disposal of your old instrument can be obtained
from your local authorities, from waste disposal companies or from your
local dealer.

2.1 Front ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
6￭￭￭￭￭￭￭￭ 944 Professional UV/VIS Detector Vario (2.944.0010)
2 Overview of the instrument
2.1 Front
1
2
Figure 1 Front
1Flow-through cell 2Standby indicator

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument
944 Professional UV/VIS Detector Vario (2.944.0010) ￭￭￭￭￭￭￭￭ 7
2.2 Rear
Figure 2 Rear
1Power switch
For switching the instrument on and off.
I = On
O = Off
2Control connection socket
Not Used.
3Analog connection socket
Output for analog signals.
4UV connection socket
For connecting the cable of the UV lamp.
5VIS lamp connection cable 6UV lamp connection cable
7Lamp cooling element 8Power socket
For connecting the power cord.
9Service connection socket
Connector for service.
10 USB 2 connection socket
For connecting additional USB devices.
11 USB 1 connection socket
For connecting additional USB devices.
12 PC connection socket
For connecting the USB cable to the PC.
13 VIS connection socket
For connecting the cable of the VIS lamp.

3.1 Setting up the instrument ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
8￭￭￭￭￭￭￭￭ 944 Professional UV/VIS Detector Vario (2.944.0010)
3 Installation
3.1 Setting up the instrument
3.1.1 Packaging
The instrument is supplied in protective packaging together with the sepa-
rately packed accessories. Keep this packaging, as only this ensures safe
transportation of the instrument.
3.1.2 Checks
Immediately after receipt, check whether the shipment has arrived com-
plete and without damage by comparing it with the delivery note.
3.1.3 Location
The instrument has been developed for operation indoors and may not be
used in explosive environments.
Place the instrument in a location of the laboratory which is suitable for
operation and free of vibrations and which provides protection against
corrosive atmosphere and contamination by chemicals.
The instrument should be protected against excessive temperature fluctua-
tions and direct sunlight.
The distance between the rear of the instrument and the wall must be
large enough to ensure air circulation on the cooling plate.
3.2 Setup configurations
The 944 Professional UV/VIS Detector Vario can be used as a detector
with the instruments of the 940 Professional IC Vario and 930 Compact IC
Flex family. For many applications with photometric detection, a post-col-
umn derivatization with the 943 Professional Thermostat / Reactor Vario
(2.943.0110) is required. To build such a system, you will need a 944 Pro-
fessional UV/VIS Detector Vario and the following instruments:
￭any 940 Professional IC Vario, or
any 930 Compact IC Flex
￭a 943 Professional Thermostat / Reactor Vario
￭optionally: a 942 Extension Module Vario for sample preparation
￭optionally, if the reagent is pumped with a high-pressure pump: a Met-
rosep BP 1 Guard/2.0 backpressure column (6.1015.100)
An IC system with photometric detection and post-column derivatization
can be set up in various configurations.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation
944 Professional UV/VIS Detector Vario (2.944.0010) ￭￭￭￭￭￭￭￭ 9
2.944.0010
2.943.0110
2.940.xxxx
2.942.xxxx
2.940.xxxx
2.942.xxxx
2.944.0010
2.943.0110
6.2061.100
6.2061.110
2.940.xxxx
2.930.xxxx
2.944.0010
2.943.0110
2.942.xxxx
6.2061.110
6.2061.100
2.930.xxxx
6.2061.120
2.942.xxxx
6.2061.100
2.944.0010
2.943.0110
6.2061.110
Figure 3 Setup configurations
Notes on the setup configurations
If you want to set up the instruments in two stacks, we recommend you
order the following accessories to protect the instruments of the second
stack:
￭Bottle holder (ProfIC) (6.2061.100)
￭Base tray with sensor for Professional IC instruments (6.2061.110)
If you would like to stack a 930 Compact IC Flex on a 944 Professional
UV/VIS Detector Vario, a 943 Professional Thermostat / Reactor Vario
and/or a 942 Extension Module Vario, then you will need the System Con-
nector (6.2061.120) to accommodate the different base sizes.

3.3 Base tray and bottle holder ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
10 ￭￭￭￭￭￭￭￭ 944 Professional UV/VIS Detector Vario (2.944.0010)
3.3 Base tray and bottle holder
3.3.1 Basic information on base tray and bottle holder
The base tray (6.2061.110) and bottle holder (6.2061.100) protect IC
instruments from dust, dirt and leaking fluids. The supply bottles for eluent
and auxiliary solutions can be positioned neatly on the bottle holder.
In a complex IC system, several different instruments may be used, such as
an analyzer, an extension module and a detector. These instruments can
be set up in one or more stacks. We recommend that a base tray and bot-
tle holder be mounted for each stack of IC instruments.
The bottle holder and base tray must be removed or set up if one of the
following instruments is to be mounted on or under a 940 Professional IC
Vario:
￭One or more 942 Extension Module Vario
￭Or another instrument with the same-sized footprint
3.3.2 Mounting base tray and bottle holder (optional)
The base tray and bottle holder come fully assembled on a new ion chro-
matograph. To install an Extension Module on the ion chromatograph,
remove the bottle holder and put it back on top of the topmost instru-
ment. To install an Extension Module under the ion chromatograph,
remove the base tray and set it under the lowest instrument.
3.3.2.1 Removing/mounting the base tray
Remove the base tray to install another instrument under the IC instru-
ment.
CAUTION
Do not pinch capillaries or leak sensor cables
Capillaries are fed through the guide ducts between the base tray and
the instrument. Pinches in the leak sensor cable or the capillaries may
lead to malfunctions.
￭Unplug the leak sensor cable before you remove the base tray.
￭Remove all the capillaries from the capillary ducts before you remove
the base tray.
Removing the base tray
Prerequisites
￭The instrument is switched off.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Installation
944 Professional UV/VIS Detector Vario (2.944.0010) ￭￭￭￭￭￭￭￭ 11
￭The bottle holder is cleared.
￭All of the cable connections on the rear have been disconnected.
￭The capillaries are removed from the guide ducts between the instru-
ment and the base tray.
￭There are no loose parts in the instrument.
Accessories
￭3 mm hex key (6.2621.100)
123
Figure 4 Removing the base tray
1Tilt the instrument sideways and lay it down flat.
2Loosen the 4 cylinder screws with the 3 mm hex key. Remove the cyl-
inder screws and their washers.
3Remove the base tray.
Always mount the base tray under the lowermost instrument of the stack.
Mounting the base tray
Prerequisites
￭The instrument is switched off.
￭The bottle holder is cleared.
￭All of the cable connections on the rear have been disconnected.
￭There are no loose parts in the instrument.
￭The instrument is lying on its side, and the bottom surface is visible.
Accessories
￭3 mm hex key (6.2621.100)

3.3 Base tray and bottle holder ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
12 ￭￭￭￭￭￭￭￭ 944 Professional UV/VIS Detector Vario (2.944.0010)
13
2
Figure 5 Mounting the base tray
1Mount the base tray in such a way that the openings in the base tray
match exactly the screw threads in the bottom of the instrument.
2Slide the washers onto the cylinder screws. Insert the cylinder screws
with the washers and tighten them with the 3 mm hex key.
3Set the instrument back up on the base tray.
Stack other instruments in the required order. Mount the bottle holder
(6.2061.100) onto the topmost instrument on the stack (see "Mounting
the bottle holder", page 13).
3.3.2.2 Removing/mounting the bottle holder
Remove the bottle holder if you want to install another instrument onto
the IC instrument.
Removing the bottle holder
Prerequisites
￭The instrument is switched off.
￭The bottle holder is cleared.
￭Drainage tubing is disconnected from the drainage tubing connection
of the bottle holder.
￭The capillaries are removed from the guide ducts between the instru-
ment and the bottle holder.
Accessories
￭3 mm hex key (6.2621.100)