NuAire NuWind NU-C200V Manual

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
NUWIND CENTRIFUGE
Models
NU-C200V
NU-C200R
NU-C200V-E
NU-C200R-E
Operation & Maintenance Manual
July, 2017
Revision 7
(115 VAC, 60 Hz) (230 VAC, 50 Hz)
Manufactured by:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, Minnesota USA 55447
Toll Free: 1-800-328-3352
In MN: 763-553-1270
Fax: [phone]

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
You have just purchased one of the finest Laboratory Centrifuges available. With proper care, maintenance
and laboratory procedure, this centrifuge will provide you years of productive service. Please read this manual
carefully to familiarize you with proper installation, maintenance and operation of the centrifuge. Other
reference and guideline materials are available on our website, www.nuaire.com.

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
NUWIND Centrifuge
TABLE OF CONTENTS
Section No. 1 ..........................................................General Information
Section No. 2 ..........................................................Models Specifications and Accessories
Section No. 3 ..........................................................Warranty
Section No. 4 ..........................................................Shipments
Section No. 5 ..........................................................Installation Instructions
5.1 ............................................................................ Location
5.2 ............................................................................ Set-up Instructions
5.3 ............................................................................ Certification Testing Methods and Equipment
Section No. 6 ..........................................................Operating the NU-C200
6.1 ............................................................................ First Start / Powering
6.2 ............................................................................ Operation / Interaction
Section No. 7 ..........................................................General Maintenance
Section No. 8 ..........................................................Error Messages, Troubleshooting, Option-Diagnostics & Performance
Verification
Section No. 9 ..........................................................Electrical/Environmental Requirements
Section No. 10 ........................................................Disposal and Recycle
Compliance Certificate ...........................................Declaration of Conformity
Annex 1.0 ...............................................................Theoretical Basics of Centrifugation
Annex 2.0 ...............................................................Accessary Specifications
ELECTRICAL SCHEMATICS
BCD-17000 ......................................NU-C200V / NU-C200V-E
BCD-17001 ......................................NU-C200R / NU-C200R-E

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NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | U.S.A | ph: 763.553.1270 | fx: 763.553.0459 | tf: 800.328.3352 | www.nuaire.com
NUWIND Centrifuge
1.0 General Information
1.1 Description
The NUWIND 2.0 is a laboratory benchtop series of centrifuges that maximizes productivity while minimizing laboratory
required bench space. The centrifuge functionality allows the separation of substances of different densities held in
suspension or emulsion in a liquid using centrifugal force.
The NUWIND 2.0 centrifuge is available in two models, one ventilated and the other refrigerated.
The NU-C200V ventilated model has a unique ventilation system to maximize performance within laboratory ambient
temperature conditions.
The NU-C200R refrigerated model adds precise temperature control to further maximize performance.
The refrigeration system refrigerant used is CFC-free to meet regulations of refrigerant use.
Centrifuges NUWIND 2.0: ventilated and refrigerated models
Intended use
The centrifuge is designed and manufactured for use with rotating accessories supplied by NuAire, Inc. It is suitable for
separating substances of different densities held in suspension, or emulsion in a liquid.
The maximum density of substances should be 1.2 g/ml.
The centrifuge must be used in an optimal state of operation and maintenance. The non-observance of the rule of use may
threaten the health of users or third persons, but it may also cause damages to the unit and/or surrounding equipment.
Users of the equipment must be trained on good centrifugation practices, as well specific usage of the NUWIND models and
Accessories. Any use outside the framework defined above is considered non-compliant.
NuAire, Inc. assumes no responsibility for injury due to improper use.
CAUTION
!

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1.2 Safety Instructions
The operator must observe the following precautions when using the centrifuge:
Ensure stability and perfect levelling of the centrifuge.
Check the correct mounting of the rotor and its accessories before starting a centrifugation cycle.
Check tube resistance at maximum applied centrifugal force: chemical resistance to centrifuged products and
mechanical strength at the centrifugal force applied thereto.
Check the condition of the tubes and remove damaged tubes.
Use only the rotors and accessories authorized by the manufacturer NuAire, Inc.
Swing-out rotors: install four (or two) buckets; never perform any cycle with missing buckets.
Maintain and control accessories.
Balance the load of the rotor around the axis of rotation.
Respect the maximum density of 1.2 g / ml, in particular in case of a cycle at full speed.
Limit volume in case of excess density.
Install the centrifuge in a ventilated area, on a horizontal rigid support to absorb the vibrations generated by the
centrifuge.
During operation, the centrifuge must not be moved or be subject to impact.
Do not attempt to open the lid while the rotor is spinning.
Do not attempt to neutralize the motorized lid lock
Do not lean over the centrifuge during the spin cycle.
Do not stay within a space of 10 inch around the centrifuge longer than necessary.
Do not leave potentially dangerous materials inside the free space.
Use aerosol-barrier accessories when centrifuging bio hazardous material.
Condensation may form inside the centrifuge when it is moved from a cold environment to a warm environment.
Allow the centrifuge to warm up for two hours before use.
Prohibitions:
The centrifuge is not designed for the following uses and environments.
The following are prohibited:
Flammable, explosive, toxic and radioactive materials
Materials which may react, causing a hazard
Contaminated materials not contained in an aerosol-barrier container
Rotating rotors and accessories that have exceeded their maximum use.
Rotating rotors and accessories that are not maintained according to the instructions in this manual,
or showing signs of wear and/or corrosion.
Rotating rotors and accessories not supplied by NuAire, Inc.
Explosive environments
Radioactive environments
Only operate a centrifuge after taking all necessary safety measures.
Rotors and buckets should be removed from use in case of mechanical fault
trace, or corrosion. These elements have a life cycle duration, engraved on
their visible part: To maintain safe conditions, it is imperative to replace them
when the recommended duration is reached.
CAUTION
!

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1.3 Explanation of Symbols
Safety alert symbol indicates a potentially hazardous situation which,
if not avoided, could result in death or serious injury
CAUTION used without the safety alert symbol indicates a potentially
hazardous situation which, if not avoided, may result in property damage.
Safety alert symbol indicates a potentially hazardous situation which,
if not avoided, may result in minor or moderate injury.
Pinching: This warning symbol indicates the presence of a risk of
pinching when handling the lid.
Note:
Used for important information
Potential electrical hazard, only qualified person to access
Biohazard
Ground, Earth
Lead Free
CAUTION
!
CAUTION
WARNING
!


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2.0 Models, Specifications and Accessories
2.1 Specifications
Description
Laboratory Centrifuge
Environmental Conditions
For indoor use only
Environment Temperature Range: : 60⁰F - 85⁰F (15⁰C - 30⁰C)
Environment Humidity: Maximum relative humidity 80% for
temperatures up to 31⁰C decreasing
linearly to 50% relative humidity at 40⁰C
Environment Altitude: 6562 Feet (2000 meters) above sea level
Maximum capacity
4x625 ml x kg / bucket, 4 x buckets
Models
Ventilated
Refrigerated
Catalogue Numbers
NU-C200V
NU-C200V-E
NU-C200R
NU-C200R-E
Supply Voltage
(Depending on the model)
115 V 10%
60 Hz
230 V 10%
50Hz
115 V 10%
60 Hz
230 V 10%
50Hz
Nominal intensity (A)
8.5 A
4 A
17 A
8.5 A
Maximum Speed (rpm)
15,300 rpm
(Depending upon the rotor)
18,000 rpm
(Depending upon the rotor)
Maximum Relative Centrifugal Force (g)
22,380 xg
30,980 xg
Total Power Consumed
750 Watt
1600 Watt
Dissipated Heat (Btuh) (1 Btuh = 0.29307 W)
3071 Btuh
6824 Btuh
Fluid Coolant / Charge
Not applicable
R404A / 0.35 kg
Ambient Operating Temperature
20⁰C ± 2⁰C
20⁰C ± 2⁰C
Temperature adjustment range
N/A
-10 ° C to 40 ° C
Temperature control precision
N/A
± 2 ° C
Dimensions on bench (H x W x D)
347x457x604 mm
13.7 x 18.1 x 22 inches
322 x 675 x 582 mm
12.7 x26.7 x23 inches
Net weight (excluding rotor)
71 kg /156 lbs.
110 kg / 242lbs.
Packing (H x W x D) / Weight
500x600x800 mm
19.8 x 23.8 x 31.7 / inches
87 kg / 191 lbs.
500x800x800 mm
19.8 x 31.7 x 31.7 inches
126 kg / 277 lbs.
Maximum sound level
60 dB(A)
55 dB(A)
Integrated systems
Imbalance detection sensor
Motorized lid lock
Rotor speed control
Imbalance detection sensor
Motorized lid lock
Rotor speed control
Control System
Microprocessor
Microprocessor
Acceleration slopes
9
9
Braking slopes
10
10
Speed control precision
± 10 rpm
± 10 rpm
Number of programs
99
99 + Pre-cooling + Pre-warming
Optimal settings refrigeration
N/A
+4 ° C temperature with
NU-RX500 rotor can be
maintained at 4,200RPM at an
ambient temperature of
20⁰C ± 2⁰C.

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2.2 Accessories
References
Description
Lot
NU-RX500
Swing-Out rotor 4x 500 ml (2 liters)
One unit
NU-B500
500 ml buckets
Set of 4
NU-L500
Lid 500 ml
Set of 4
NU-2T5
Tube insert 23x 5 ml
Set of 4
NU-2T10
Tube insert 19x 10/15 ml
Set of 4
NU-2T15CL
Conical tube insert 11 x 15 ml
Set of 4
NU-2T50CL
Conical tube insert 4 x 50 ml
Set of 4
NU-2T50C*
Conical tube insert 5 x 50 ml
Set of 4
NU-2T50S
Self-standing conical tube insert 5 x 50 ml
Set of 4
NU-2T100
Round bottom bottle insert 1x 100 ml
Set of 4
NU-2T200
Round bottom bottle insert 1x 200 ml
Set of 4
NU-2T400
Round bottom bottle insert 1x 400 ml
Set of 4
NU-2T265
Flat bottom bottle 1x 625mL
Set of 4
NU-BXDW
Microplate Bucket X
Set of 4
NU-RHDW
Microplate Rotor H
One unit
NU-BHDW
Microplate Bucket H
Set of 2
NU-RX250
Swing-Out rotor 4x 250 ml (1 liter)
One unit
NU-B250
250 ml bucket
Set of 4
NU-L250
Lid 250 ml
Set of 4
NU-1T5
Tube insert 15x 7 ml
Set of 4
NU-1T10
Tube insert 12x 10 ml
Set of 4
NU-1T15C
Conical tube insert 7x 15 ml
Set of 4
NU-1T50C
Conical tube insert 3 x 50 ml
Set of 4
NU-1T50S
Self-standing conical tube insert 3 x 50 ml
Set of 4
NU-1T100
Round bottom bottle insert 1x 100 ml
Set of 4
NU-1T200
Round bottom bottle insert 1x 200 ml
Set of 4
NU-RA24-2
24x 2 ml 45° angle rotor
One unit
NU-RA30-2
30x 2 ml 45° angle rotor
One unit
NU-RA48-2
48x 2 ml 45° angle rotor
One unit
NU-RA16-5
16x 5 ml 45° angle rotor
One unit
NU-RA8-50
8x 50 ml 25° angle rotor
One unit
(*) these inserts cannot be used with Bio-containment lids (NU-B500)

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3.0 Warranty
NuAire, Inc. warrants NU-C200 Series NUWIND Centrifuge that it will repair F.O.B. its factory or furnish without charge
F.O.B. its factory a similar part to replace any material in its equipment within 60 months after the date of sale if proved to
the satisfaction of the company to have been defective at the time it was sold provided that all parts claimed defective shall
be returned, properly identified to the company at its factory, charges prepaid. Factory installed equipment or accessories
are warranted only to the extent guaranteed by the original manufacturer, and this warranty shall not apply to any portion
of the equipment modified by the user. Claims under this warranty should be directed to NuAire, Inc. setting forth in detail
the nature of the defect, the date of the initial installation and the serial and model number of the equipment.
This warranty shall not apply to any NuAire product or part thereof which has been subject to misuse, abuse, accident,
shipping damage, improper installation or service, or damage by fire, flood or acts of God. If the serial number of this
product is altered, removed or defaced as to be illegible, the Warranty shall be null and void in its entirety.
The warranty is for the sole benefit of the original purchaser and is not assignable or transferable. Prior to returning any
item, for any reason, contact NuAire for a Return Authorization Number. This number must accompany all returns. Any
product shipped to NuAire without this number will be returned refused shipment or collect freight.
4.0 Shipments
NuAire takes every reasonable precaution to assure that your NUWIND Centrifuge arrives without damage. Motor carriers
are carefully selected and shipping cartons have been specially designed to insure your purchase. However, damage can
occur in any shipment and the following outlines the steps you should take on receipt of a NuAire NUWIND Centrifuge to be
sure that if damage has occurred, the proper claims and actions are taken immediately.
4.1 Damaged Shipments
4.1.1 Terms are factory, unless stated otherwise. Therefore, it is important to check each shipment before acceptance.
4.1.2 If there is visible damage, the material can be accepted after the driver makes a notation on the consignee's copy
of the freight bill. Then an inspection must be made to verify the claim against the carrier. This inspection is the
basis of your filing the claim against the carrier.
4.1.3 If concealed damage is found it is absolutely necessary to NOTIFY THE FREIGHT AGENT AT ONCE and request an
inspection. Without this inspection, the transportation company may not accept a claim for loss or damage. If the
carrier will not perform the inspection, an affidavit must be prepared stating that he was contacted on a certain
date and that he failed to comply with the request. This along with other papers in the customer's possession will
support the claim.

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5.0 Installation Instructions
5.1 Handling and transport
The package containing the centrifuge needs to be handled by suitable means (pallet truck, etc.).
Please ensure that the people in charge of handling are qualified to handle the lifting equipment.
Maintain the following conditions during transport:
Temperature: -20 to 50 ° C
Relative humidity: <90%, under the dew point
5.2 Unpacking
Remove the cardboard packaging made of 2 parts (a lid and a bottom).
The NUWIND centrifuge was packed with great care to prevent transport hazards. Before disposing of the packaging of the
centrifuge, and its spinning accessories, please make sure you are in possession of the following:
5.2.1 Packing list
1) or
2)
NUWIND 2.0 Centrifuge Model
1) Ventilated or
2) Refrigerated
Compliance Certificate
User Manual (Flash Drive)
Power Cord
Manual release key
Food grade grease
5.2.2 Preparation of rotor and accessories
The rotor and the 4 buckets may have been packed inside the centrifuge in order to reduce the volume of packing
material. However, before any utilization, make sure to remove the packing foam before turning the unit on for the
first time.
These packing foams allow the rotor to be centered inside the bowl without any constraint or forces applied directly
on the motor shaft or without damaging any internal part in the bowl.
1. Insert the allen wrench in the hole on the front panel to manually
open the centrifuge
2. Turn it clockwise until the lid opens up
3. Remove the allen wrench from the hole
4. Remove all the packing foam inside the bowl
5. Remove the buckets and put them on a table next to the centrifuge
6. Remove the rotor with both hands and put it on a table
(it shouldn’t be locked on the shaft) next to the centrifuge
7. Remove all the different layers of packing foam
8. If needed, clean the bowl if any residual packing material or dust is
sitting at the bottom
CAUTION
!
2
3
1
NUWIND
Centrifuge
Operation and
Maintenance
Manual
Declaration
Of
Conformity

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5.2.3 Description
1. Lid
2. Window
3. Control panel
4. Electronic warhead
5. Main switch
6. Cooling unit (refrigerated model only)
7. Electrical outlet
5.3 Site preparation
Prepare a stable, clean and levelled support (e.g. laboratory bench). This support must be able to support the weight of the
centrifuge and its use, and be firm enough so as not to generate or maintain vibration. Such vibrations would degrade the
results of the centrifugation, and may result in an unbalanced error.
A clearance of 10 inch (250mm) around the centrifuge should be
arranged according to IEC 61010-2-020: No potentially hazardous
material, nobody or no object shall be deposited within the free
space.
Open space around the centrifuge
It is recommended that no less than two people are present
to manually lift the centrifuge onto the laboratory bench.
5.4 Electrical environment
The centrifuge requires must be 230V/50Hz or 115V/60Hz, single phase, (current rating varies per centrifuge model,
reference Electrical/Environmental Requirements).according to the manufacturer sheet on the back of the unit.
The centrifuge must be plugged into an outlet with protective earthing connection with the standard power cord.
The electrical outlet into which the centrifuge is connected should be readily accessible for maintenance purposes.
Do not position the centrifuge to prevent access to the power cord.
The power cord plug serves as the disconnect and should remain readily accessible. The electrical outlet should be on its
own branch circuit, protected with a circuit breaker at the distribution panel near the centrifuge.
10”
10”
Access area
10”
CAUTION
!
2
1
3
5
4
7
6

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6.0 Operating the NU-C200
6.1 First Start / Powering
The first start must fulfill the conditions of the IEC 61010-2-020 safety standard

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6.1.1 Rotor assembly
1. Clean and lubricate the motor shaft by depositing a thin film
of the food grade grease supplied.
2. Place the rotor above the drive shaft.
3. Slide the rotor on the drive shaft.
4. A click indicates that the rotor is locked.
5. Clean and lubricate the pins by depositing a thin film of the
food grease supplied.
6.1.2 Position the buckets
Swing-out rotors are used with round buckets or micro titration buckets.
1. Place the buckets in the corresponding numbered locations.
6.1.3 First powering
Activate the switch on the bottom right of the control center.
Lid Open Switch
Lid Open Switch
On/Off Switch position
Under Control Center
On/Off Switch position
Under Control Center

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6.1.4 Using the interface
6.1.5 Opening the lid
On the first start, the lid may not open right away due to a long storage in a cold environment.
In this case, warm-up the jacks by repeated manual shifting of the lid downwards.
To open the lid, press the button .
6.1.6 Rotor loading and balancing
Position the 4 numbered buckets in their corresponding positions
on the rotor. Each bucket should pivot freely in its slot.
Place the inserts corresponding to the tubes to be centrifuged in
the four buckets
The buckets must be symmetrically distributed.
For better balance, each numbered bucket must be placed in the
corresponding position marked on the rotor.
Each position on the rotor must be equipped with its bucket.
Do not use a rotor without its complete set of four buckets.
Maximum loads according to the type of rotor are specified in the
annex.
The centrifuge tolerates an imbalance of 15grams.
If the imbalance is higher than the tolerated threshold, an
imbalance detection system stops the centrifuge automatically.
The following message appears: "Imbalance" and the rotor stops in
free wheel mode without braking system.
Excessive imbalance is likely to damage the turning components
and/or the centrifuge.
Loading the buckets
Allowed:
Forbidden :
Loading the rotor
Allowed:
Symmetrical loading
Forbidden:
Incomplete loading
Asymmetrical loading
1. Touch screen for direct access to various functions.
2. Single button for easy operation.
This button allows the validation, the start of a cycle, but also
the interruption of a cycle, and the output of a menu.
The functions accessible through this single button are
activated as required by the microcontroller.
2
1

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6.1.7 Quick start-up
Before using the centrifuge, make sure it has been properly installed.
6.2 Operating
6.2.1 Settings
This section is accessible through the Admin section. It allows the creation and/or modification of a program and the
customization of the centrifuge.

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6.2.2 Create / edit a program name
Visual
Instruction
Control
Select the program to create/edit
Note: 99 programs are available,
from 01 to 99.
The program can be modified when the
protection icon is an open lock:
Edit the title screen by pressing on the title.
Enter the new title
Save the new title by pressing "Save".
Note:
To delete the entire text:
To exit the menu without saving changes:
Confirm saving
Save any changes
Exit without saving changes:
The program has been saved.
(The lock is not activated).

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6.2.3 Set program settings
Visual
Instruction
Control
Select the program to edit
Select the desired parameter by pressing
the icon:
Acceleration slope (from 1 to 9)
1: slowest acceleration
9: fastest acceleration
Braking slope )from 0 to 9)
0: no braking at all
1: slowest deceleration
9: fastest deceleration
Centrifuge speed (in RPM or RCF)
Cycle time
Setpoint temperature (only on the
refrigerated model)
Change the value (e.g. speed).
Confirm
Confirm settings
Save any changes
Exit without saving changes:

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6.2.4 Set speed in RPM or RCF
Visual
Instruction
Control
Access the speed setting key
Press on the icon RPM to modify the unit from
RPM to RCF.
Enter the RCF (g) value desired,
Confirm
Note: The rotor name and radius will be
Displayed below the picture of the rotor.
The program will now display the RCF value (g).

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6.2.5 Protect the settings of a program
From an Admin profile, a program can be locked to prevent any modifications of the settings
Visual
Instruction
Control
Select the program to lock
Press on the open lock for 4 seconds.
The lock is now closed.
The program is now protected.
Note: To remove the protection, enter the
program through an Admin user
profile and press on the closed lock
for 3 seconds.
It will then re-open. You are now
able to modify the program.
In the list of programs, a lock appears in
front of the program

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6.2.6 Centrifuge: Use a program
Turn on the centrifuge. The switch is in the lower right portion.
Any user can access a program (user, admin, service tech)
Visual
Instruction
Control
Select the program you want to use
Load the rotor and buckets as instructed
in 6.1.6
Close the lid
Start the cycle
Centrifugation starts.
The progression bar appears
Open the lid when finished
Remove the tubes.