Motic SWIFTLINE MAE-31R User manual

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Inverted Microscope
Instruction Manual
Model MAE-31R

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TABLE OF CONTENTS
SECTION
II Specifications ................................................................................................................4
III Setting-up the Instrument ..............................................................................................5
IV Assembling the microscope Input voltage .....................................................................6
1Installing the lamp.................................................................................................................... 6
2Mounting the condenser........................................................................................................... 6
3Installing Objectives................................................................................................................. 7
4Mounting the eyepieces........................................................................................................... 7
VMicroscopic procedure Interpupillary distance adjustment ............................................7
1Diopter adjustment................................................................................................................... 8
2Centering the Condenser......................................................................................................... 8
3Centering the lamp................................................................................................................... 9
4Brightfield microscopy.............................................................................................................11
5Phase-contrast microscopy.....................................................................................................11
VI Photo Procedure..........................................................................................................13
VII Troubleshooting Table.................................................................................................15
VIII Care and maintenance ................................................................................................16
1Lenses and filters....................................................................................................................16
2Cleaning of painted or plastic components..............................................................................16
3When not in use......................................................................................................................16
IX Warranty......................................................................................................................17

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1. Lamp Socket Clamp Screw Knob 8. Objectives
2. Lamp House Cover Clamp Screw 9. Revolving Nosepiece
3. Annular Diaphragm 10. Coaxial Coarse/Fine Focus Knob
4. Condenser Diaphragm Lever 11. Torque Adjusting Ring
5. Condenser Focus Knob 12. Filter Retaining Ring
6. Condenser Lens 13. Hexagonal Centering Screwdrivers (x2)
7. Stage Plate Insert

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1. Vertical Photo Port
2. Interpupillary Distance Scale
3. Diopter Adjustment Ring
4. Eyepiece
5. Optical Path Selector Lever
6. Filter Slider
7. Lamp House
8. Field Diaphragm Lever
9. Condenser Clamp Screw
10. Phase Slider
11. Condenser Clamp Holder Screw
12. Condenser Centering Screws
13. Stage Plate
14. Fluorescence Filter cassette Mount
15. Power Switch
16. Light Intensity Control Dial
17. Brightness Indicator (LED Segmented Display)
II Specifications

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•Magnification Ratio: 40X –400X
•Eyepiece: N-WF 10X/22mm with diopter adjustment, +/- 5 diopter
•Objectives: Infinity Corrected
Magnification
N.A.
W.D. (mm)
Plan Achromat 4X
0.10
23.5
Plan Achromat Phase 10X
0.25
7.5
Plan Achromat Phase LWD 20X
0.40
7.0
•Condenser: Extra Long Working Distance N.A. 0.3 / W.D. 72mm
•Electrical Specifications:
Input: IEC 90-240V~, 35W, 50-60Hz, 0.6A Max (UL / CE)
Lamp: 6V, 30W Halogen (100hrs)
Fuse: 250V T2.5A
III Setting-up the Instrument
Working environment

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•The location should be free from dust, moisture, chemical vapors and mechanical vibrations.
•Do not situate the instrument in a warm and/or humid environment.
•Locate the instrument where the operator’s line of vision is not directed towards a window, a lamp or a
well-lit bright wall. The quality of the viewed image from the microscope will deteriorate where there is
significant ambient light.
IV Assembling the microscope Input voltage
•Automatic voltage selection works with electrical outlets worldwide. However, always use a power cord
that is rated for the voltage used in your area and that has been approved to meet local safety
standards. Using the wrong power cord could cause fire or equipment damage.
•The use of an extension cord is discouraged and may damage the electrical components of the
microscope.
•In order to prevent electric shock, always turn the power switch on the power supply off before
connecting the power cord.
1 Installing the lamp
•In order to prevent electric shock always turn the power switch off and unplug the power cord before
replacing the lamp.
•Loosen and remove the lamp housing cover clamp screw using a coin. Then remove the lamp house
cover.
•When installing the lamp, do not touch the glass surface of the
lamp with bare fingers. Doing so
will cause fingerprints, grease, etc., to burn onto the lamp surface,
reducing the illumination provided by the lamp. If surface is contaminated, wipe it clean using lens
tissue.
•Firmly insert the lamp into the socket pinholes until it reaches the limit, be careful not to tilt the
lamp when mounting.
•Close the cover and fasten with it with lamp housing cover clamp screw.
2 Mounting the Condenser
Power Cord
Lamp Housing Cover
Clamp Screw
Power Switch

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•Mount the condenser with the index marks and aperture diaphragm lever facing forward. Then secure
it with the clamp screw.
•Insert the phase annular diaphragm slider with centering hexagonal socket head screws facing
the front. See the images above for reference.
3 Installing Objectives
•Remove the stage plate insert from the stage.
•Install the objectives into the nosepiece so that the magnification increases with clockwise rotation of the
revolving nosepiece.
•Replace the stage plate insert.
4 Mounting the eyepieces
•Remove the dust caps from the eyepiece tubes.
•Insert the eyepieces into the eyepiece tubes.
•If the rubber eye guards are to be used, fit them in the groove around the eyepiece.
V Microscopic procedure Interpupillary distance adjustment
•Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.
Condenser Clamp
Screw
Phase Annular
Diaphragm
Slider
Condenser

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•Adjust the interpupillary distance so that both the right and left field of view become one.
•This adjustment will enable the user to observe the specimen with both eyes
1 Diopter adjustment
•Diopter adjustment compensates for differences in vision between the left and right eyes. In addition to
making observation through both eyes easier, this adjustment also reduces the
extent to which focusing is lost when the objective magnification is changed. In particular,
this occurs when a low power objective is used.
•Before adjusting the diopter, bring a specimen into focus using the 10x objective.
•Turn the diopter compensation ring on each eyepiece until the adjustment ring is adjusted to
“0” position.
•Position 40x objective into the optical path and bring the specimen image into focus by turning
the coarse and fine focus knobs.
•Position either 4x or 10x objective into optical path. Without adjusting the fine and coarse focus knobs,
turn the diopter rings on the eyepieces so that the specimen images in the left and right eyepieces are
focused individually.
•Repeat the above step twice.
2 Centering the Condenser
•Set position of the phase annular diaphragm slider in center opened position, of the condenser.
•Fully open the condenser diaphragm by moving lever to the open “O” position.
Diopter adjustment “0”
position
Center Open Position

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•Turn the light intensity down and switch to the 10x objective.
•Close the field of view diaphragm, 3/4 of the way. This lever is located on the lamp housing. This will
close down the iris diaphragm in the lamp housing and become visible through the eyepiece field of
view.
•Turn the condenser focus knob up or down until the iris edges sharpen in the field of view.
•Adjust the condenser centering screws so that the center of the field diaphragm image matches the
center of the field of view. This adjustment is easier to make if the field diaphragm size is reduced
slightly smaller than the eyepiece field of view.
•IMPORTANT: For normal observation, the edges of the
diaphragm should be just outside the field of view.
3 Centering the lamp
•Set the Phase annular diaphragm slider in the open center position.
Condenser centered
Center Open Position
Condenser not centered
Condenser centered
Condenser Centering Screws

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•Close the condenser diagram down completely –moving lever to C.
•Fully open the field of diaphragm and
remove the diffuser filter slide.
•Loosen, don’t remove the lamp socket clamp screw using the knob. This will allow the knob to move
around with little resistance.
•Holding the lamp socket clamp screw, move the lamp filament image to the center of the condenser
diaphragm iris. The filament will be visible looking down from above through the top of the condenser.
Once centered tighten down the lamp socket clamp screw.
•After finishing the above lamp centering procedure, re-insert the filter slider.
Lamp Socket Clamp
Screw Knob

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4 Brightfield microscopy
•Set the Phase annular diaphragm slider in the centered open position.
•Bring the specimen image into focus.
•Adjust the opening of the field of view diaphragm, for normal observation the size of the diaphragm
should be just outside the edge of the field of view.
•The condenser aperture diaphragm is provided for adjusting the numerical aperture (N.A.) of the
illuminating system of the microscope. It is important because it determines the resolution of the image,
contrast, depth of focus and brightness.
•Closing down the aperture diaphragm will lower the resolution and brightness but increase the contrast
and depth of focus. By closing down the N.A. of the condenser to 2/3 of the N.A. of the objective, an
image of suitable contrast will be obtained.
5 Phase-contrast microscopy
•Phase contrast objectives are labeled “Ph”
•Fully open the aperture diaphragm. Position the Phase annular diaphragm slider to 10 -20. Set slider to
40 when using a 40x (Ph3) objective. See picture below.
•Bring the 10x (Ph1) objective into optical path.

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•Remove either eyepiece from the eyepiece tube and insert the phase centering telescope
in its place.
•Loosen the locking screw of the centering telescope eyepiece and slide out until both the phase plate
image of the objective and the annular diaphragm image of the phase slider are in focus.

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•If the objective phase plate and the annular of the slider do not coincide, use the two hexagonal
screwdrivers supplied with the microscope to bring the slider annular ring to the center of the
phase plate, so that the image of the annular diaphragm is concentric with the phase plate image.
•If the slider annular ring image is moved from the phase plate image in the objective, a low
phase contrast image will result.
•For phase contrast microscopy at the maximum contrast, use GIF (Green interference filter)
in the optical path.
•Place the filter in the designated retaining ring above the phase annular diaphragm slider.
Filter selection
Filter type
Procedure
GIF (Green interference) 546nm
For phase contrast and contrast adjustment with black
and white film
NCB (Neutral Color Balance)
Blue
For general microscopy and color photomicrography
VI Photo Procedure
•The optical path selector lever can be used to set the optical path to either the Binocular tube 100:0 or
Binocular tube/vertical tube 20:80 (observation: photo).
•Before starting, make sure all prior steps in this manual are followed.
•In order to attach a camera to the microscope, an additional camera adapter must be purchased.
See picture below for reference. Also refer to the parts and accessories section on page 17.

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•To attach the C0.5X adapter to your camera, simply screw onto your C-Mount camera. Remove the
cap on the camera port of your microscope, by loosening the knurled screw. Then attach the C0.5X and
lock into place with the knurled screw.
•Then follow the directions of use, for your camera.

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VII Troubleshooting Table
The troubleshooting table below contains the most frequently encountered problems and their possible
causes.
Optical and Operating Problems
Problem
Possible Cause
Vignetting or uneven brightness
in the field of view or field of view
only partially visible
Lamp not installed properly
Filter slider in intermediate position
Phase slider not in click-stop position
Incorrect condenser mounting
Condenser is set too low
Condenser is not centered
Field diaphragm closed too far
Aperture diaphragm closed too far
Revolving nosepiece not clicked
into position
Optical path selector lever in
intermediate position
Dust or dirt in field of view
Aperture diaphragm closed too far
Field of view diaphragm image not
focused on specimen surface
Dust or dirt on specimen’s surface
Image quality:
No image under phase contrast
or details cannot be viewed
Brightfield objective being used
Phase annular diaphragm not in
optical path
Phase annular diaphragm and
objective phase symbol do not match
Slider annular ring image has
moved away from the objective
phase plate image
Field of view diaphragm image not
focused on specimen surface
Thickness of specimen holder is
outside the compensating range
of objective
Eye strain or fatigue
Interpupillary distance not adjusted
Diopter adjustment not made
Inadequate illumination
Field of view of left and right eyepiece differ
Electrical
Lamp does not light
Power supply not plugged in
Lamp not installed
Lamp burnt out –100hrs Average Life
Inadequate brightness
Specified lamp not being used
Lamp blows out immediately
Specified lamp not being used
Lamp flickers
Connectors are not securely connected
Lamp near end of service life
Lamp not securely plugged into socket
Never attempt either of the following actions, since doing so will damage
the focusing mechanism:
oRotate the left and right knob while holding the other.
oTurning the coarse and fine focus knobs further than their limit.

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VIII Care and maintenance
1 Lenses and filters
•To clean lens surfaces or filters, first remove dust using compressed air. If dust still persists, use a
soft/clean brush or gauze.
•A soft gauze or lens tissue lightly moistened with pure alcohol should only be used to remove grease or
fingerprints.
•Use petroleum benzine to clean immersion oil.
•Use petroleum benzine only to remove immersion oil from objective lenses.
•Because petroleum benzine and absolute alcohol are both highly flammable, be careful handling around
open flame.
•Do not use same area of gauze or tissue, to wipe more than once.
2 Cleaning of painted or plastic components
•Do not use organic solvents (thinners, alcohol, ether, etc.). Doing so could result in discoloration or in
the peeling of paint.
•For stubborn dirt, moisten a piece of gauze with diluted detergent and wipe clean.
3 When not in use
•When not in use, cover the instrument with dust cover and store in a place low in humidity where mould
is not likely to form.
•Store the objectives, eyepieces and filters in a container or desiccator with drying agent.

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IX Warrany
Motic Swift Line Warranty
The Motic Swift Line 5 Year Warranty assures that the microscope is guaranteed against defects in material
and workmanship for 5 years from the purchase date of the product. Electrical components are covered for 1
year; video components are covered for one year after purchase. Normal wear, routine maintenance, light
bulbs, power supplies, rechargers, batteries, fuses, cords, add-on accessories, damage resulting from repair
by unauthorized parties, accident, alteration, shipping, misuse or abuse is not covered. Determination of
warranty is at the technician’s discretion.
Defective products covered by the warranty will be repaired free of charge when they are returned, postpaid.
Warranty Process
An official Return Material Authorization (RMA) number must accompany any return, including
a copy of the original sales or purchase receipt, with the RMA number also clearly written on
the exterior of the shipping box. Microscope(s) must be shipped back to originating location or
sales office. A detailed description of the difficulty must be enclosed with the returned unit,
shipped freight pre-paid to Motic Instruments USA, Inc. Motic Instruments USA, Inc, after
evaluation and warranty determined, will repair, or replace at no charge and return. If, after
evaluation, failure was caused by misuse, alterations, accident, or abnormal conditions of
operation, an estimate of repairs will be submitted for your approval prior to work being
performed.
For Product and Customers outside the US
For customers living outside the United States, Motic Instruments USA, Inc. will provide
standard warranty service. However, all inbound & outbound shipping cost, including taxes and
tariffs, are the responsibility of the consumer.
For questions about warranty and repairs:
•Toll Free: 1-800-275-3716
•Email: [email protected]
For Digital Technical Support, including software:
•Toll Free: 1-877-901-4141
•[email protected]