NanoEnTek ADAM MC2 User manual

Instruction Manual
A New Standard of Automated Cell Counter
Copyright NanoEnTek, Inc. All rights reserved.
ADAM
MC2

All the materials in this user manual are protected by Korean and international copyright laws. They cannot be
reproduced, translated, published or distributed without the permission of the copyright owner.
ADAM-MC2 Instruction Manual
Website : www.nanoentek.com
E-mail : [email protected]
Manufactured by
NanoEnTek, Inc.
851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel. [phone]
Fax. [phone]
NanoEnTek America, Inc.
240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA
Tel. [phone]
Fax. [phone]
The information in this manual is described as accurately as possible.
Firmware and software changes and updates may change without prior consent or notication.
Copyright © 2018 by NanoEnTek Inc.
All rights reserved. Published in Korea.
Documentation: NESMU-AMC2-001E
Revision history: V.0.0 DEC 2018

Table of contents
Introduction
General description
Technology
Basic principle of counting
Setting
Setting
Wi
Update
Product Contents
ADAM-MC2
AccuChip kit
Upon receiving the instrument
Maintenance and cleaning
Trouble shooting
Warranty
Technical specications
Product list
Safety precautions
Safety symbols
Warnings
Technical Support
General Operation
Introduction
Sample preparation
Counting cell
Measure
Run sample
Result analysis
Result analysis -Error code
Data
Data list
Edit
Image
Mail
Power o
Lock
Power o
Getting Started
Environmental requirements
Power on and initial display
Error messages during booting
Count setting
Product Description
Front view of ADAM-MC2
Rear view of ADAM-MC2
2
3
4
5
5
5
6
7
8
8
9
10
11
12
12
15
16
17
18
19
20
21
22
23
23
24
24
25
26
27
28
29
30
31
32
33

2
Introduction
General
Description
The ADAM-MC2, an automated cell counter, is a benchtop automated cell
counter that performs cell count and viability measurements using
AccuStain Solution.

ADAM-MC2, A New Standard of Automated Cell Counter 3
Introduction
Technology Until now, cell counting and viability measurement for many types of cells
have been performed manually using hemocytometer with Trypan Blue
exclusion method, which is to distinguish viable cells from non-viable cells.
One drawback of this method, however, is the propensity for the staining
of artifacts; another drawback is that the naked eye can only dierenti-
ate between cells in a limited concentration range in the hemocytometer
chamber. This combined with the potential problem of cell aggregation
and limited sample volume leads to the common variation of counts nor-
mally associated with this method.
To address these problems, NanoEnTek has developed the ADAM-MC2,
which is based on a uorescent microscopy technique for counting cells.
The ADAM-MC2 utilizes sensitive uorescence dye staining, LED optics and
CMOS detection technologies to make the cell analysis more accurate and
reliable.
To count cells using ADAM-MC2, the cells are mixed with a Propidium Io-
dide (PI) stain and directly pipetted on to a disposable plastic chip. The
chip is then loaded onto a precision stage. An ADAM-MC2 system is au-
tomatically focused onto the chip and cells that have been stained are
recorded by a sensitive CMOS camera. The image results are automatically
processed generating the cell count which is displayed on the front of the
instrument. Simple. Fast. Accurate. Reliable.
AccuChip
CMOS Camera
Emission Filter
Dichroic Filter
Objective Lens
Excitation lter
Lenses
Green LED Mirror

4
Introduction
Basic principle
of counting
ADAM-MC2 is based on staining mammalian cell DNA with a uorescent
dye, Propidium Iodide (PI). PI does not enter cells with intact membranes
or active metabolism. In contrast, cells with damaged membranes or with
inactive metabolism are unable to prevent PI entering the cell. As a result,
the nuclei of non-viable cells will only be stained. The ADAM-MC2 pro-
vides two kinds of staining solutions. AccuStain Solution T for the total cell
counting is composed of the uorescent dye (PI) and lysis solution. Ac-
cuStain Solution N for the non-viable cell counting is composed of the
uorescent dye and PBS.
In order to measure the total concentration of cells, the plasma mem-
branes of all the cells must be disrupted to stain all the Nuclei with PI. The
process of disrupting and staining is achieved by treatment with AccuStain
Solution T.
In the second solution, live cells remain intact and are not stained.
Only the non-viable cells are stained and detected. After treatment, the
prepared cells will be loaded into the chip. The viability will be automati-
cally calculated in the ADAM-MC2 software after each measurement of the
total cells and the non-viable cells.
Add PI
T Channel
A: Total cell / B: Non-viable cell
N Channel
AccuChip 4 Channel
AccuChip 2 Channel
*Viability(%)= X 100
A
(A-B)
Total cell count Non-viable cell count
Non-viable cellNon-viable cell
Viable cellViable cell PI-stained nuclei
PI-stained nuclei PI-stained nuclei
T1 N1 T2 N2
Add PI + Iysis sol.

ADAM-MC2, A New Standard of Automated Cell Counter 5
Introduction
ADAM-MC2
AccuChip kit
Upon receiving
the instrument
The contents of the ADAM-MC2 are listed below:
The contents of the ADAM-MC2’s AccuChip Kit are listed below:
• Examine the instrument carefully for any damage incurred during transit.
•Ensure that all parts of the instrument including accessories listed above
are included with the product.
• Any damage claims must be led with the carrier.
• The warranty does not cover in-transit damage.
• Upon receipt, store AccuChip at room temperature. AccuStain Solution
should be stored at 2~8°C
Item Quantity
Main device 1
Instruction manual 1
USB hub 1
Wi dongle 1
Power cord 1
Adapter 1
AccuChip Kit 1
Calibration Bead 1
Item AccuChip2x Kit
(Cat. No: AD2K-200)
AccuChip4x Kit
(Cat. No: AD4K-200)
AccuStain Solution
(Cat. No: ADR-1000)
Disposable
Chip 200pcs (2 channel) 200pcs (4 channel) N/A
Solution T 12.5 mL x 2ea 12.5 mL x 2ea 12.5 mL x 4ea
Solution N 12.5 mL x 1ea 12.5 mL x 1ea 12.5 mL x 2ea
Available
test Q’ty
Min. 200 test/kit Min. 400 test/kit
Max. 400 test/kit
(Only total cell count)
Max. 800 test/kit
(Only total cell count)
Product Contents

6
Introduction
Front view of
ADAM-MC2
The front view showing various parts of the ADAM-MC2 is shown below:
Control buttons Description
① Door Slide holder is inserted and ejected.
② Power Power on / o.
③ LCD Display processes and results.
④ START Performs all procedures of automatic counting.
⑤ LOCK
Protects the alignment of stage from external shock when
ADAM-MC2 is being moved.
Lock ADAM-MC2 before turning it o or moving it.
⑥ EJECT Ejects the slide holder from ADAM-MC2. Functions as unload.
Product Description
Door
Power
LCD
⑥
④
⑤
①
②
③

ADAM-MC2, A New Standard of Automated Cell Counter 7
Introduction
Rear view of
ADAM-MC2
The rear view showing various parts of the ADAM-MC2.
Port Description
①USB Port Port for software update and save the data.
③Power Plug Connects ADAM-MC2 power cord to wall outlet
Product Description
①USB Port ②PC Port ③Power plug
Do not use the PC port. This port does not recognize USB.
!CAUTION
②

8
Introduction
Power on and
Initial Display
Getting Started
Environmental
requirements
To ensure correct operation and stable performance,
install the ADAM-MC2 in a location which meets the following conditions:
1. Use at room temperature between 20 and 35 °C
• Not recommended for cold room use (≤ 4 °C).
2. Do not expose the device to direct sunlight.
3. Do not subject the device to direct or continuous vibration.
4. Do not subject the device to intense magnetic or electromagnetic elds.
5. Do not install the device in high-humidity environment.
6. Location of device should be free from corrosive gases or other corrosive
substances.
7. Ensure minimal contact with dust or other airborne particles.
8. Allow a 10 cm (4 inches) minimum space around the device for proper
airow.
9. Do not place any objects on the device.
1. Check the connection of ADAM-MC2 and power cord.
2. Press the power button for 2~3 seconds.
If you get an error message,
please contact your local distributor or [email protected].
If booting is successful and no errors are detected,
the home screens will be displayed as below.
At low temperature (≤10 °C), allow the device to warm up for 10 minutes at
ambient temperature before use.
!CAUTION
•Do not tilt the device too much in the forward when connecting the power cord.
•Do not move the device after connecting power cord.
- When you connect the power cord to ADAM-MC2 even without power on the device,
it will go through self diagnostic tests.
!CAUTION

ADAM-MC2, A New Standard of Automated Cell Counter 9
Introduction
Error Messages
during booting
[System State]
Error code Cause
0x00000C00 Failure of X-axis sensor
0x00007000 Failure of Y-axis sensor
0x00008000 Failure of Z-axis sensor
0x06000000 Failure of Locking module sensor
Getting Started
It appears when booting is not working properly.
Turn o main power and restart device.
If this message still appears after restarting,
contact your local distributor or [email protected].

10
IntroductionGetting Started
Count setting Set the conditions in the ‘Setting’ tap before counting.
[AccuChip]
Set the AccuChip according to you are using.
[Cell size]
Set the minimum and maximum size of cell.
[Dilution factor]
When diluting sample, set the Dilution factor.
Factor values for the AccuStain Solution is already applied.
!CAUTION
2Ch4Ch
Accuchip
4Ch 2Ch
Accuchip
Cell size Min
5
Max 8
0
Dilution factor
T1 N1 T2 N2
1.0

ADAM-MC2, A New Standard of Automated Cell Counter 11
IntroductionGeneral Operation
Introduction Instruction are provided in this section for preparing the sample with
AccuStain Solution for use with disposable AccuChip for automated cell
count using the ADAM-MC2.
Please check the procedure of sample preparation and testing below.
For more detailed information, please refer to the next page.
2. Load the mixed sample. Then, wait 1 minute for the sample settling.
1. Mix the sample with AccuStain Solution.
3. Insert AccuChip. Get the result.
AccuStain Solution
50 μL
Sample
50 μL
OR
- Result -
2 channel: 23μL
4 channel: 13μL
T channel: Total cell
N channel: Non-viable cell

12
Introduction
T1 N1 T2 N2
When you load of the sample mixture to the AccuChip, please be careful
not to make bubbles.
!NOTE
Concentration out of this range will result in errors.
Refer to page 17 for more information about errors.
!NOTE
Counting cell
General Operation
Sample
preparation
3. Thoroughly mix the cell pellet by vortexing.
4. Check visually if any cell clumps or agglomerates are remained.
[AccuChip 2x]
[AccuChip 4x]
Total Cell Non-viable Cell
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution T.
2) Voltex the tube vigorously.
3) Load 23 µL sample mixture to the
AccuChip on Tchannel. Then, wait
1 minute for the sample settling.
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution N.
2) Voltex the tube vigorously.
3) Load 23 µL sample mixture to the
AccuChip on Nchannel. Then, wait
1 minute for the sample settling.
Total Cell Non-viable Cell
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution T.
2) Voltex the tube vigorously.
3) Load 13 µL sample mixture to the
AccuChip on T1 or T2 channel.
Then, wait 1 minute for the sample
settling.
1) Add 50 µL of your sample to 50 µL
supplied AccuStain Solution N.
2) Voltex the tube vigorously.
3) Load 13 µL sample mixture to the
AccuChip on N1 or N2 channel.
Then, wait 1 minute for the sample
settling.
Total
Total
T/N2 T/N1
Non-viable
Non-viable
1. Cultivate the required number of cells.
2. Add an appropriate volume of growth media or PBS to dilute to a nal
concentration of 5 x 10 cells/mL to 4 x 10 cells/mL.
4 6

ADAM-MC2, A New Standard of Automated Cell Counter 13
Introduction
T1 N1 T2 N2
T1 N1 T2 N2
T1 N1 T2 N2
General Operation
Counting cell
Correct volume
Low volume
[Sample loading error]
Be cautious of loading the correct volume of the sample into AccuChip.
The instrument will not detect low or high sample volumes.
!WARNING
Avoid bubbles which may negatively aect the result.
!CAUTION

14
IntroductionGeneral Operation
Counting cell
[AccuChip insert error]
Completely insert AccuChip face up, in the direction of the arrow on the
slide. The instrument will not detect if slides are inserted incorrectly.
See pictures below for proper insertion.
!WARNING
• Please insert or remove the AccuChip when the slide holder is fully ejected.
• When the test is nished, please remove the AccuChip from the slide holder.
!CAUTION
(O )
(X )
(X )
(X )
Correctly inserted
Upside down inserted
Not fully inserted
Wrong end inserted

ADAM-MC2, A New Standard of Automated Cell Counter 15
IntroductionMeasure
Run Sample Start counting process by pressing‘START’.
It may take about 2 minutes longer for auto focus at the initial test.
While the test is in progress, you can check the cell images of each channel.

16
IntroductionMeasure
Result Analysis The result will be displayed after being automatically calculated by ADAM-
MC2 software.
Title Number of
Total cell
Number of
Non-Viable cell Viability
HeLa_300μM H2O2 T1 (1.10E6) N1 (5.50E5) 50%
HeLa_100μM H2O2 T2 (2.20E6) N2 (5.50E5) 75%
•The viability will be automatically calculated by the ADAM-MC2 software after
each measurement of the total cells and the non-viable cells.
• First, the total cell number and second, non-viable cell number were measured
and then the cell viability is calculated as subtracting non-viable cell counting
numbers from total cell counting.
* 1.10E6 = 1.10 X 10 cells/mL
6
CLOSE