NanoEnTek ADAM-SCC2 User manual

Instruction Manual
Automated Somatic Cell Counter
Copyright NanoEnTek, Inc. All rights reserved.
ADAM
SCC2

All the materials in this user manual are protected by Korean and international copyright laws. They cannot be
reproduced, translated, published or distributed without the permission of the copyright owner.
ADAM-SCC2 Instruction Manual
Website : www.nanoentek.com
E-mail : [email protected]
Manufactured by
NanoEnTek, Inc.
851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel. [phone]
Fax. [phone]
NanoEnTek America, Inc.
240 Bear Hill Road, Suite 101, Waltham, MA 02451, USA
Tel. [phone]
Fax. [phone]
The information in this manual is described as accurately as possible.
Firmware and software changes and updates may change without prior consent or notication.
Copyright © 2019 by NanoEnTek Inc.
All rights reserved. Published in Korea.
Documentation: NESMU-ASC2-001E
Revision history: V.0.0 AUG 2019
V.1.0 DEC 2019

Table of contents
Introduction
General description
Technology
Somatic Cell Count Kit
Setting
Setting
Wi
Update
Power o
Lock
Power o
Product Contents
ADAM-SCC2
SOMA Chip kit
Upon receiving the instrument
Maintenance and cleaning
Trouble shooting
Warranty
Technical specications
Product list
Safety precautions
Safety symbols
Warnings
Technical Support
General Operation
Introduction
Sample preparation
Counting cell
Measure
Run sample
Result analysis
Result analysis -Error code
Data
Data list
Edit
Image
Mail
Getting Started
Environmental requirements
Power on and initial display
Error messages during botting
Count setting
Product Description
Front view of ADAM-SCC2
Rear view of ADAM-SCC2
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3
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2
Introduction
General
Description
ADAM-SCC2 is an automated somatic cell counter integrated with uores-
cence optic and image analysis software. ADAM-SCC2 counts the number
of somatic cells to determine the quality of raw milk with unique image
cytometer technology. ADAM-SCC2 can be used as an ideal equipment in
the research eld, dairy farms, milk manufacturing facilities, and veterinary
institutes working with somatic cell analysis. It will be helpful to manage
the milk quality to provide the best grade of milk.

ADAM-SCC2, Automated Somatic Cell Counter 3
Introduction
Technology High levels of somatic cells cause disagreeable taste and reduce storage
life in dairy products. These somatic cell counts (SCCs) are accepted as an
international standard for the measurement of milk quality. For this reason,
somatic cell counts are readily available to a dairy farmer in most countries.
Standard reference method for enumeration of somatic cells in raw milk is
the direct microscopic somatic cell count (DMSCC). However, it needs the
training and skill of analysts for accuracy, precision, and reproducibility of
this method. ADAM-SCC2 is fully compatible with the DMSCC. It is com-
posed of the disposable plastic microchips and staining solutions, a uo-
rescence microscopic optics equipped with a CMOS camera, and an image
analysis system. It utilizes the capillary ow of the microuidic chamber
by the surface modication of hydrophilicity. Microuidic technology of
disposable microchips provides low reagent consumption and combining
with the ready-to-use reagent makes daily work easy.
ADAM-SCC2 is not only easy to use but oers the same repeatability and
accuracy as the conventional expensive device.
SOMA chip
CMOS Camera
Emission Filter
Dichroic Filter
Objective Lens
Excitation lter
Lenses
Green LED Mirror

4
Introduction
Somatic Cell
Count Kit
Somatic Cell Count Kit of ADAM-SCC2 is composed of Propidium Iodide (PI)
for counting somatic cells. It can be used without diluting raw milk.
Measuring range of cell density is 0.05 ~ 1.15 x 106cells/mL.
Each SCC-Solution has 25 mL reagent of somatic stain solution. Simply add
100 μL volume of the raw milk sample and 100 μL stain solution in the
1.5 mL tube. Once the experiment is complete the results can be printed
through the optional thermal print. Printed number indicates cell concen-
tration ( x 1000/mL) in each channel.
Store kit box upright and at room temperature. Expiration date of stain so-
lution is written on the bottom of the kit box (yy-mm-dd). Be sure to check
the expiration date before using. Follow the exact steps detailed in the
Instructions for Use section.
ㆍSOMA Chip 2X : Load 23 μL/Channel, 2 test/Chip
ㆍSOMA Chip 4X : Load 13 μL/Channel, 4 test/Chip

ADAM-SCC2, Automated Somatic Cell Counter 5
Introduction
ADAM-SCC2
SOMA Chip kit
Upon receiving
the instrument
The contents of the ADAM-SCC2 are listed below:
The contents of the ADAM-SCC2’s Somatic Cell Count Kit are listed below:
• Examine the instrument carefully for any damage incurred during transit.
•Ensure that all parts of the instrument including accessories listed above
are included with the product.
• Any damage claims must be led with the carrier.
• The warranty does not cover in-transit damage.
• Upon receipt, store SOMA Chip at room temperature.
• SCC-Solution should be stored at 2~8℃.
Item Quantity
Main device 1
Instruction manual 1
USB hub 1
Wi dongle 1
Power cord 1
Adapter 1
SOMA Chip Kit 1
Calibration Bead 1
Barcode scanner 1
Item SOMA Chip 2x Kit
(Cat. No: CRS-K01)
SOMA Chip 4x Kit
(Cat. No: CRS-K02)
Disposable Chip 50pcs (2 channel) 100pcs (4 channel)
SCC-Solution 25 mL x 1ea 25 mL x 2ea
Available test Q’ty 100 test/kit 400 test/kit
Product Contents

6
Introduction
Front view of
ADAM-SCC2
The front view showing various parts of the ADAM-SCC2 is shown below:
Control buttons Description
① Door Slide holder is inserted and ejected.
② Power Power on / o.
③ LCD Display processes and results.
④ START Performs all procedures of automatic counting.
⑤ LOCK
Protects the alignment of stage from external shock when
ADAM-SCC2 is being moved.
Lock ADAM-SCC2 before turning it o or moving it.
⑥ EJECT Ejects the slide holder from ADAM-SCC2. Functions as unload.
Product Description
Door
Power
LCD
⑥
④
⑤
①
②
③

ADAM-SCC2, Automated Somatic Cell Counter 7
Introduction
Rear view of
ADAM-SCC2
The rear view showing various parts of the ADAM-SCC2.
Port Description
①USB Port Port for software update and save the data.
③Power Plug Connects ADAM-SCC2 power cord to wall outlet.
Product Description
①USB Port ②PC Port ③Power plug
Do not use the PC port. This port does not recognize USB.
!CAUTION
②

8
Introduction
Power on and
Initial Display
Getting Started
Environmental
requirements
To ensure correct operation and stable performance,
install the ADAM-SCC2 in a location which meets the following conditions:
1. Use at room temperature between 20 and 35 °C.
• Not recommended for cold room use (≤ 4 °C).
2. Do not expose the device to direct sunlight.
3. Do not subject the device to direct or continuous vibration.
4. Do not subject the device to intense magnetic or electromagnetic elds.
5. Do not install the device in high-humidity environment.
6. Location of device should be free from corrosive gases or other corrosive
substances.
7. Ensure minimal contact with dust or other airborne particles.
8. Allow a 10 cm (4 inches) minimum space around the device for proper
airow.
9. Do not place any objects on the device.
1. Check the connection of ADAM-SCC2 and power cord.
2. Press the power button for 2~3 seconds.
If you get an error message,
please contact your local distributor or [email protected].
If booting is successful and no errors are detected,
the home screens will be displayed as below.
At low temperature (≤10 °C), allow the device to warm up for 10 minutes at
ambient temperature before use.
!CAUTION
•Do not tilt the device too much in the forward when connecting the power cord.
•Do not move the device after connecting power cord.
- When you connect the power cord to ADAM-SCC2 even without power on the device,
it will go through self diagnostic tests.
!CAUTION

ADAM-SCC2, Automated Somatic Cell Counter 9
Introduction
Error Messages
during booting
[System State]
Error code Cause
0x00000C00 Failure of X-axis sensor
0x00007000 Failure of Y-axis sensor
0x00008000 Failure of Z-axis sensor
0x06000000 Failure of Locking module sensor
Getting Started
It appears when booting is not working properly.
Turn o main power and restart device.
If this message still appears after restarting,
contact your local distributor or [email protected].

10
IntroductionGetting Started
Count setting Set the conditions in the ‘Setting’ tap before counting.
Somachip
Somachip
[SOMA Chip]
Set the SOMA Chip according to you are using.
[Cell size]
Set the minimum and maximum size of cell.
[Dilution factor]
When diluting sample, set the Dilution factor.
Factor values for the SCC-Solution is already applied.
!CAUTION
Cell size Min
5
Max 8
0
Dilution factor
T1 N1 T2 N2
1.0
2Ch4Ch
4Ch 2Ch

ADAM-SCC2, Automated Somatic Cell Counter 11
IntroductionGeneral Operation
Introduction Instructions are provided in this section for preparing the sample with SCC-
Solution for use with disposable SOMA Chip for automated somatic cell
count using the ADAM-SCC2.
Please check the procedure of sample preparation and testing below.
For more detailed information, please refer to the next page.
2. Load the mixed sample. Then, wait 1 minute for the sample settling.
1. Mix the raw milk sample with SCC-Solution.
3. Insert SOMA Chip. Get the result.
- Result -
- 2 channel: 23μL
- 4 channel: 13μL
SCC-Solution
100 μL
Milk sample
100 μL

12
IntroductionGeneral Operation
Sample
preparation
Counting Cell
1. Prepare some raw milk sample, SCC-Solution, tube, pipette, and tips.
2. Thoroughly mix the raw milk sample.
1. Add 100 μL of raw milk sample and 100 μL of SCC-Solution in the tube.
(1:1 ratio)
2. Mix the raw milk sample and SCC-
Solution by turning the tube upside
down 3-5 times.
3. Load the cell sample onto the chip.
Then, wait 1 minute for the sample
settling.
- 2 channel: 23μL
- 4 channel: 13μL
Raw milk Tube SCC-Solution
13μL
SOMA Chip 4X
Ensure that no bubbles enter each channel.
!NOTE
SCC-Solution
100 μL
Milk sample
100 μL

ADAM-SCC2, Automated Somatic Cell Counter 13
IntroductionGeneral Operation
Counting cell [Sample loading error]
Be cautious of loading the correct volume of the sample into SOMA Chip.
The instrument will not detect low or high sample volumes.
!WARNING
T1 N1 T2 N2
Correct volume
Low volume
Avoid bubbles which may negatively aect the result.
!CAUTION
T1 N1 T2 N2
T1 N1 T2 N2

14
IntroductionGeneral Operation
Counting cell
[SOMA Chip insert error]
Completely insert SOMA Chip face up, in the direction of the arrow on the
slide. The instrument will not detect if slides are inserted incorrectly.
See pictures below for proper insertion.
!WARNING
• Please insert or remove the SOMA Chip when the slide holder is fully ejected.
• When the test is nished, please remove the SOMA Chip from the slide holder.
!CAUTION
(O )
(X )
(X )
(X )
Correctly inserted
Upside down inserted
Not fully inserted
Wrong end inserted

ADAM-SCC2, Automated Somatic Cell Counter 15
IntroductionMeasure
Run Sample Enter a title for each channel of the slide.
Click the title section, then scan the cow ID with a barcode scanner to au-
tomatically enter as the title.
Start counting process by pressing‘START’.
It may take about 2 minutes longer for auto focus at the initial test.
While the test is in progress, you can check the cell images of each channel.
9010 7436

16
IntroductionMeasure
Result Analysis The result will be displayed after being automatically calculated by ADAM-
SCC2 software.
No Title Count
T1 120 1.04 x 10E6
N1 125 1.15 x 10E5
T2 130 4.68 x 10E5
N2 143 2.42 x 10E5
CLOSE