NanoEnTek JuLi Stage User manual

JuLI™Stage, Real-Time Cell History Recorder 1
User Manual
Copyright NanoEnTek Inc. All rights reserved.

2
All the materials in this user guide are protected by Korean and international
copyright laws. They cannot be reproduced, translated, published or distributed
without the permission of the copyright owner.
JuLI™ Stage User Manual
Website : www.julistage.com
E-mail : [email protected]
Manufactured by : NanoEnTek Inc.
851-14, Seohae-ro, Paltan-myeon, Hwaseong-si, Gyeonggi-do, 18531, Korea
Tel : [phone]
Fax : [phone]
The information in this manual is described as accurately as possible. The changes
involved in rmware and software may be applied without notication.
Copyright ©2014 by NanoEnTek Inc. All rights reserved. Published in Korea.
Documentation: NESMU-JST-001E
Revision history: V.0.0 Date: OCT 2014
V.0.5 Date: MAY 2015
V.1.0 Date: SEP 2015
V.1.5 Date: MAR 2016
V.2.0 Date: MAY 2017

JuLI™Stage, Real-Time Cell History Recorder 3
Table of contents
Product components
Product overview
Features
Description
- Front view
- Side view
- Rear view
- Control box
Installation
- Unpacking the instrument
- Remove the lter change
lock module
- Install the instrument
- Place the instrument
- Operating environment
- Cautions
User interface
- GUI
Basic operation
- Moving
- Selection
- Position zoom
- Zoom
- Focus
- Size bar
- Auto Focus
Operation
- Preview menu
- Option menu
- Data menu
- Setting menu
4
5
6
7
7
7
8
8
9
9
9
10
12
13
13
14
14
16
16
17
19
20
21
21
22
28
28
36
44
47
50
52
53
54
56
58
60
61
62
63
65
66
67
Quick operation guide
- Position Calibration (for Vessel type)
- Stitching Image Calibration (Stitching & Map mode)
- Image capture, save and overlay
- Operation type-1
(for Selecting multiple samples and use the Auto focus)
- Operation type-2
(for Select single samples and use the Manual focus)
Cleaning and Maintenance
Troubleshooting
Warranty
Safety precautions
Product specications
Ordering information
Technical support

4
The JuLI™ Stage, Real-Time Cell History Recorder is shipped with the following components.
Please check all components listed below when the instrument is delivered.
PC will be shipped separately because it is optional.
If any item is missed of damaged, contact your local distributor or e-mail [email protected].
Product components
JuLITM Stage main instrument
1 EA
1 SET
1 EA 1 EA
5M, 2EA
2 EA 3 EA
10x lens is factory-installed with the
JuLI™ Stage main instrument.
2 EA 4 EA
Control Box
User manual Quick manual
Repeater
Connection Cable Objective lens (4x, 10x, 20x)
Power cord with 4 adaptor cords
(4 pcs/set, for U.S./Canada/Taiwan/Japan,
Europe or UK)
USB Cables (20 ~ 43 cm)
NanoEnTekInc. (HQ) 5,Digital-ro 26-gil, Guro-gu, Seoul, 152-740, Korea / Tel: [phone], Fax:[phone]
NanoEnTekUSA Inc. 5627 Stoneridge Drive Suite 304, Pleasanton, CA 94588, USA / Tel:[phone], Fax: [phone]
www.nanoentek.com/ [email protected]
NESMQ-JST-001E(V.0.0)
Quick manual
User Manual
Copyright NanoEnTekInc.All rights reserved.
2 EA
Ferrite core

JuLI™Stage, Real-Time Cell History Recorder 5
Product overview
A fully-automated, real-time cell history recorder that acquires cell images directly from various cell culture plates (6 to
384 well) slides and dishes in an incubator. JuLI™ Stage supports multi-channel uorescent colors, multiple objective
lenses, and sensitive lter-based optics to optimize for various live cell assays. In addition, it enables users to support
quantied cellconuence results with low variation and the growth curve using image-based analysis with bright eld.
Compact, compatible and Real-Time Cell History Recorder of live cell imaging from inside your cell
culture incubator!
Suitable for the following applications:
• Live cell imaging (time lapse) • Cell growth monitoring
• Cell culture quality control • Cell culture optimization
• HCS cell imaging analysis (6 to 384 well) • Stem cell development
• Cell-based assay optimization • Cell proliferation
• Image stitching • Cell cytotoxicity
• Angiogenesis • Cell migration study
• Scratch-wound assay • Cell dierentiation
• Neurite growth
• Angiogenesis
• Protein expression
• Kinetic imaging and cell dierentiation

6
Features
The JuLITM Stage a Real-Time Cell History Recorder, oers you the following benets,
Automated imaging system
The JuLI™ Stage provides a live cell imaging using automatic focus and a precise one by one multi-well control system.
- Fully automated and motorized X-Y scanning stage
- Auto focusing on every single position
- Image tiling with a stitching function
Sensitivity and expandability
The sensitive CCD camera, multi-channel uorescence and magnication (4, 10 & 20 X) provide the powerful detection
capabilities required for various cell-based assays.
- High-sensitivity monochrome CCD
- Multi-channel uorescence imaging (3 color uorescence GFP, RFP and DAPI and bright channel)
- User-changeable objective lens (4, 10 & 20 X)
Versatile and Powerful S/W
The JuLI™ Stage software allows you to generate live cell imaging and analysis easily.
- Image capturing and time-lapse recording with multi-position scanning
- Navigation function to gure out the optimal position
- Image stitching function to scan the whole well at once
Compatibility and easy to use
The compact and ready to use system can be operated in an incubator and it can be controlled by the JuLI™ Stage
software from outside.
- Compact and compatible to CO2incubator
- 23” full HD All-in-one touch screen PC control
- Simple steps for the system setup and operations
- Compatibility with various brands of well plates by auto adjustment function

JuLI™Stage, Real-Time Cell History Recorder 7
Description
Front view
Side view
[Front view]
[Side view]
LED (Bright mode)
Vessel holder
Automatic X-Y stage
Lens holder
Cover
Main body
Used to protect test samples from external light. When you use the JuLl™ Stage
in an incubator, the cover can block out light from the outside.
Front view of the JuLl™Stage. The main body is composed of LED, Filters
(Fluorescence mode) and optic parts.
LED (Bright mode)
Vessel holder
Automatic X-Y
stage
Lens holder
LED is used for bright image viewer.
This is a holder for sampling plates. The capacity is 6 well to 384 well.
The automatic X-Y stage makes it possible for you to move to the position
you want.
This is a holder for changeable lens.
Main Body
(LED, Fluorescence Filters & optic parts)
Cover

8
Description
Rear view Fan
Connection port
Fan
Connection port
Power inlet
Power inlet
Machinery cooling system.
Connect the JuLI™ Stage main instrument with the control box.
Connect the instrument to an electrical outlet using the supplied power
cord and the appropriate plug, based on the electrical outlet conguration
in your country.
[Rear view]
Control box When the PC and the control box are properly connected, the red light is
turned on.
Connect the JuLI™ Stage main instrument with the control box
Control the camera in the JuLI™ Stage main instrument.
Control the JuLI™ Stage main instrument operating.
Serial and camera
LED
Connection port
Serial port
Camera port
Connection port Camera portSerial port
Serial connection LED
Camera connection LED

JuLI™Stage, Real-Time Cell History Recorder 9
1. Unscrew the lter change lock module screw to remove it.
2. Gently pull lter change lock module screw out, away from the instrument.
3. Put the lter in the pouch to keep safe.
IMPORTANT!
Remove the Filter change lock module by turning it counterclockwise to prevent damage
of the instrument before plugging the power cord into power outlet and turning on the JuLI™ Stage.
1. Open the box and remove the material foam from the box.
2. Carefully lift the instrument out of the box, holding it by handholds in the bottom of the base.
3. Place the instrument on a at, level surface (table or incubator).
Do not lift the JuLI™ Stage by condense arm.
Installation
Unpacking
the instrument
Remove the
lter change
lock module
IMPORTANT!
Filter change lock module
Filter change lock module

10
Installation
1. Connect repeater to the PC and two USB cable, then connect to control box.
(PC Monitor Resolution: 1920 ×1080 pixels)
1-1. Plug the power cord into the rear side of the PC.
2. Connect the JuLI™Stage instrument and the Control box using the connection cable.
Connect the connection cable and control box by matching the blue and red sticker.
3. Plug the power cord of PC and instrument into the electrical outlet.
2-1. Plug the power cord into the rear of instrument.
☞ Note: Check the connection on the rear side of the PC.
☞ Note: If you connect all the components, it will look like the Image below.
☞ Note: Make sure of connection port direction of control box before connecting.
☞ Note: Be sure to use only the power cord supplied with your instrument.
Powering the instrument with an unapproved power cord may damage the instrument.
Install
the instrument
Power port USB port

JuLI™Stage, Real-Time Cell History Recorder 11
Installation
4. Turn the PC and the Control box on by pressing the Power button.
5. Double-click the JuLI™ Stage icon on Desktop. The JuLI™ Stage software starts up.
☞ Note: The JuLI™ Stage will be ready to use after initialization is completed when power on.
Install
the instrument

12
Installation
Live-cell images from various cell culture dishes are directly captured in a cell culture incubator.
This design allows an instrument to be installed in an incubator, and prevents the contamination
by maintaining a sterile environment. The intuitive graphic user interface also provides quick and
convenient use in a cell culture incubator while capturing time-lapse imaging.
The JuLI™ Stage operates in standard size of incubator. The JuLI™ Stage can be used in both inside
and outside the incubator.
Use Cover supplied if the instrument is installed on the table to protect test samples from
external light.
JuLI™ Stage installed inside of the incubator
JuLI™ Stage installed on the table
Place the
instrument into
the incubator
(or on the table)
IMPORTANT!

JuLI™Stage, Real-Time Cell History Recorder 13
Installation
• Allow at least 10 cm (4 inches) free space at the back of the instrument to allow for proper venti-
lation and prevent overheating of electronic components.
• Set up the JuLI™ Stage away from direct light sources, such as windows. Ambient room lighting
can enter the imaging path and aect the image quality.
• Place the JuLI™ Stage on a level surface away from vibrations from other pieces of equipment.
• Operating temperature range: 5 - 40°C (41 - 104°F)
• Relative humidity range: 20 - 95 %.
The JuLI™ Stage is optimized for a standard size cell culture incubator.
It is recommend that the temperature should be stabilized in case of a small size incubator before
using the instrument.
Warm up the JuLI™ Stage for 2 hours before monitoring.
The power should be on when warming up the instrument.
The well plate’s cover should be wetted by the cell culture media before monitoring.
This step is required to avoid condensation inside the well plate cover for monitoring.
Avoid exposing the JuLI™ Stage to UV light.
UV light may degrade components, including plastics. Damage from UV exposure is not covered
under the manufacturer’s warranty.
Always wipe the surfaces with ethanol-soaked paper towels.
Do not directly spray ethanol anywhere on the JuLI™ Stage.
Operating
environment
Cautions IMPORTANT!

14
User interface
The JuLI™ Stage software allows you to generate live cell imaging and analysis easily.
- Image capturing and time-lapse recording with multi-position scanning
- Navigation function to gure out the optimal position
- Image stitching function to scan the whole well or regions of the well at once
The JuLI™ Stage is a fully automated imaging system controlled by the integrated JuLI™ Stage
software. The software, accessed using the mouse, controls the automated X-Y axis stage and
features imaging and analysis tools.
GUI
Preview menu
Option menu

JuLI™Stage, Real-Time Cell History Recorder 15
User interface
GUI Data menu
Settings menu

16
Basic operation
The JuLI™ Stage can be used in combination with PC (Monitor Resolution 1920x1080) enabling
users to operate the instrument using the computer mouse and keyboard. The JuLI™ Stage basic
operations use the mouse for moving, selecting and zooming in/out. The detailed
explanations are listed below.
☞ Note: This manual describes the mouse function in Default setting (Type 1).
If necessary, you can change the mouse setting type the Setting menu.
1) Inter well: Moving among wells
Move the stage to the well you want by clicking the Right button of the mouse in both preview
and option menu.
2) Intra well: Moving within the well
The stage is moved to a position you have selected on the navigation window by clicking the
right button of the mouse in both preview and option mode.
3) Moving in view
The stage is moved to the spot you assigned in the view eld by Dragging with the right button
in both preview and option mode. The moving distance is marked by a red line as below.
The stage is moved from A-1 to A-2 by clicking the right button of the mouse.
The selected well is marked by a yellow circle as shown above.
The stage focuses in the center of the well when the well is selected. The selected image is marked
by a red square when the specic spot is selected by clicking the right button of the mouse.
Right mouse
Moving
Right: Moving
Left : Selection

JuLI™Stage, Real-Time Cell History Recorder 17
Right: Moving
Left : Selection
Basic operation
1) Selecting well
The well is marked by a grey color when the specic well is selected by clicking
The Left button. Click once to select and click once more to deselect.
☞Note: The JuLI™ Stage supports selecting function only in the Option menu.
The selecting function does not work in the Preview menu.
Left mouse
Selection
None of 24wells is selected
The selected well A-1 is marked by a grey color by press-
ing the specic well.
The rst well line is marked by a grey color when press-
ing line 1.
The “A” well line is marked by a grey color when press-
ing line A.
☞Note: Deselection: Select each well again or drag the
selected wells with the Shift key on the keyboard.
Select any well or any well lines with the left mouse button by dragging.
☞ Note: Deselection: Select each well again or drag the selected wells with the Shift key on the
keyboard.

18
Basic operation
2) Selecting position
When the specic position well is selected by clicking the Left button, the assigned position is
marked by a Green square in the Navigation window. Press the Position set button in order to
conrm.
The JuLI™ Stage supports the multi-selection functions.
Left mouse
Selection
None of the positions in the Navigation window are selected.
The Green square appears in the center of the well
when pressing the Default button.
The JuLI™ Stage allows users to select more than one position in a well
by clicking the Left button.
Click the selected position with Left button of the mouse and drag to
move the position within the well.
Click the selected position with Left button of the mouse and
drag with Shift button. Then the selected position is set with
positon that you want.

JuLI™Stage, Real-Time Cell History Recorder 19
Basic operation
Left mouse
Selection
Position
zoom
Double click the position to delete.
Press the Position Add button to save your setting
- the green square will change to a Red square.
1X zoom in
2X zoom in
4X zoom in
8X zoom in
When you press + or - button, it activates the zoom function.
The range of position zoom is from 1X to 8X.
For more details, select or check the position information.

20
Basic operation
1) Zoom-in
Scroll up in the view eld to use the zoom in the image.
The JuLI™ Stage supports zoom-in function in both the real view and captured images allowing
users to magnify specic position by scrolling up.
Mouse wheel
(Up and down)
Zoom
2) Zoom-out
After zooming in, Scroll down to zoom out of the view eld in both real view and
captured images
< 1st zoom in >
Magnied navigation
< 2nd zoom in >
Magnied navigation
< 3rd zoom in >
Magnied navigation