Nikon Eclipse E400 POL User manual

Nikon
Polarizing
Microscope
ECLIPSE
E400
POL
Instructions

Thank you for purchasing the Nikon products.
This
instruction
manual
is
written
for
the
users
of the
Nikon
Polarizing
Microscope
ECLIPSE E400 POL.
To
ensure
correct
usage,
read
this
manual
carefully
before
operating
the
instrument.
e
It
is
prohibited
to
reproduce
or
transmit
this
manual
in
part
or
whole
without
Nikon's
expressed
permission.
e
The
contents
of
this
manual
are
subject
to
change
without
notice.
e
Some
of
the
products
described
in
this
manual
may
not
be
included
in
the
set
you
have
purchased.
+
Although
every
effort
has
been
made
to
ensure
the
accuracy
of
this
manual,
if
you
note
any
points
that
are
unclear
or
incorrect,
contact
your
nearest
Nikon
representative.
Warning/Caution Symbols Used in This Manual
Although Nikon products are designed to provide you with the utmost safety during
use, incorrect usage or disregard of the instructions can cause personal injury or
property
damage.
For
your
safety,
read
the
instruction
manual
carefully
and
thoroughly
before
using
the
instrument.
Do
not
discard
this
manual
but
keep
it
near
the
product
for
easy reference.
In
this
manual,
safety
instructions
are
indicated
with
the
symbols
shown
below.
Be
sure
to
follow
the
instructions
indicated
with
these
symbols
to
ensure
correct
and
safe
operation.
Symbol Meaning
Disregarding instructions marked with this symbol may lead
WARNING
to
death
or
serious
injury.
Disregarding instructions marked with this symbol may lead
CAUTION =.
to injury or property damage.
PP
Meaning of Symbols Used on the Eguipment
Symbol Meaning
Caution for heat.
MA This marking on the lamp cover calls your attention on the
following;
e Lam cover becomes very hot during and immediately after
the illumination.
e Risk of burns. Do not touch the lamp cover during and
immediately after the illumination.
e Make sure that the lamp cover is sufficiently cool before
the lamp replacement.

YANI
1. Intended product use
This
microscope
should
only
be
used
for
microscopic
observation.
Do
not
use
it
for
any
other purpose.
2. Do not disassemble
Disassembling
may
cause
malfunction
and/or
electrical
shock.
Do
not
disassemble
any
parts
other
than
those
mentioned
in
this
manual.
If
you
notice
any
malfunction,
notify
your
nearest Nikon representative.
3. Input voltage
Make
sure
that the
input
voltage
indicated
on
the
back
panel
of
the
microscope
is
the
same
as
your
regional
voltage.
If
not
the
same,
do
not
use
the
microscope;
instead,
notify
your
nearest
Nikon
representative
immediately.
If
the
microscope
is
used
with
the
wrong
input
voltage,
a
short
circuit
or
fire
may
result,
causing the damage of the microscope.
4. Power cord
To
prevent
electrical
shock,
always
tum
off
the
power
switch
(flip
it
to
the
“O”
side)
before
connecting
or
disconnecting
the
power
cord.
Use
only
the
following
power
supply
cord
set.
Using
the
wrong
power
cord
could
result in damage or fire.
* For 100-120V area
UL
Listed,
detachable
cord
set,
3
conductor
grounding
type
SVT,
No.
18
AWG
rated
at
125V, 7A minimum.
In
case
of
using
the
extension
cord,
use
only
the
power
supply
cord
with
the
PE
(protective
earth) wire.
* For 220-240V area
3
pole
power
supply
cord
set,
which
must
be
approved
according
to
EU/EN
standards.
_
Class
I
equipment
should
be
connected
to
PE
(protective
earth)
terminal.
In
case
of
using
the
extension
cord,
use
only
the
power
supply
cord
with
the
PE
(protective
earth) wire.
5.
Heat
from
the
light
source
The
lamp
and
the
lamp
cover
become
extremely
hot.
To
avoid
burns,
do
not
touch
the
lamp
cover
while
the
lamp
is
lit
or
for
thirty
minutes
after
it
has
been
turned
off.
Furthermore,
in
order
to
avoid
the
risk
of
fire,
do
not
place
fabric,
paper
or
highly
flammable
materials
such
as
gasoline,
petroleum
benzine,
paint
thinner
or
alcohol
near
the
lamp
cover
while
it
is
lit
or
for
about
thirty
minutes
after
it
has
been
turned
off.
The
back
of
the
microscope
also
becomes
hot
during
use.
Although
this
is
not
a
malfunction,
be
careful
not
to
touch
the
back
of
the
microscope
when
it
is
hot.

[caution
1.
Only use the specified halogen lamp
The
power
supply
unit
in
the
microscope
provides
the
power
for
the
halogen
lamp
that
serves
as
the
microscope
light
source.
This
unit
can
supply
power
for
a
halogen
lamp
of
up
to
6V-30W.
The
specified
lamp
must
be
used,
otherwise,
the
microscope
could
be
damaged.
Specified lamp: 6V-30W halogen lamp (PHILIPS 5761)
Precautions for replacing the lamp
To
prevent
burns,
allow
the
lamp
to
cool
for
at
least
thirty
minutes
after
turning
off
the
power
switch before
replacing
it.
Furthermore,
to
prevent
electrical
shock
and
damage
to
the
microscope,
always
turn
off
the
power
switch
(flip
it
to
the
“O”
side)
and
unplug
the
power cord before replacing the lamp.
After
replacing
the
lamp,
be
sure
to
attach
the
lamp
cover
securely.
Never
light
the
lamp
without its cover.
Do not wet the microscope
If
the
microscope
gets
wet.
a
short
circuit
may
result
that
could
damage
it
or
make
it
extremely
hot.
If
you
accidentally
spill
a
liquid
on
the
microscope.
immediately
turn
off
the
power
switch
(flip
it
to
the
“O”
side)
and
unplug
the
power
cord.
Then
use
a
dry
cloth
to
wipe
away
the
moisture.
If
any
liquid
gets
inside
the
microscope,
do
not use
it;
instead,
notify your nearest Nikon representative.
Weak electromagnetic waves
This
microscope
emits
weak
electromagnetic
waves.
The
accuracy
of
any
precision
electronic
eguipment
may
be
adversely
affected
if
positioned
too
close.
If
the
microscope
affects
TV
or
radio
reception,
move
the
radio
or
TV
further
away
from
the
microscope.
Precautions for installation
Be
careful
not
to
pinch
your
hands
or
fingers
when
installing
the
microscope.
This
microscope
is
a
precision
optical
instrument,
and
using
or
storing
it
under
unsuitable
conditions
may
damage
it
or
may
have
an
adverse
effect
on
its
accuracy.
See
“Installation
location”
on
p.28
and
always
use
the
microscope
in
a
suitable
environment.
Precautions for moving the microscope
Securely hold the microscope by the base of
the arm from the back.
Do not grasp the focus knobs, eyepiece tube,
stage, etc. when carrying the microscope.
This could cause the part to come off,
resulting in damage to the microscope.
Be careful not to hit the lamp cover against
the other objects when lifting up or placing
the microscope.
This microscope is a precision optical
instrument, so handle it carefully and do not
Subject it to a strong physical shock. (The
accuracy of the objective in particular may be
adversely affected by even a weak physical
shock.)

CONTENTS
Warning/Caution
SymbolsUsedinThisManual
..................................................
1
N
WARNING
nr
스
2
ZN
CAUTION
....................,
3
Ш
Names
of
Component
Parts
and
Operational
Parts
a.
6
F
Microscopic
Procedure
...
se
..
.
8
EJ
Operation
of
Each
Part
…
14
EN
Coarse
and
fine
focus
KNODS
nni
14
1.
Rotation
of
the
coarse
and
fine
focus
knobs
and
vertical
movement
of
the
stage
un
…
14
2.
Adjusting
the
torque
of
the
coarse
focus
køb
ccoo
na
14
3.
Соагзе
AS
A
eee
15
Trinocular
eyepiece
tube
emme
16
1.
Ориса]
рав
зе|есвол
..................
ии.
16
2.
Vertical
如
be
adapter
cc
mm
16
3.
Optical
path
selection
lever
cicking...........................
eee
16
Diopter
adjustment
ii
17
EJ
Interpupillary
distance
adjustment
mmm
17
Condenser..............
италии,
18
1.
Focusing
and
centering
A
18
2.
Condenser
aperture
diaphragm...............
이
19
3.
Swing-out
the
top
lens
of
the
condenser
20
Field
diaphragm
.
20
A
..
..20
Е
Auto-photo
switch
(for
photomicrography)
-
21
Oil-immersion
operation
(using
an
oil-immersion
type
of
objective
and
condenser
...............
июню,
21
Onientation
of
polarizing
plates
(Polarizer
and
Analyzer)
tries
22
Focusing
and
centering
the
Bertrand
lens
.
-
ere
23
P-CL
1/44
&
tint
plate
..
23
Centering
the
objectives
ic
…
24
K
Circular
graduated
stage
for
polarizing
microscope...
run
emma
24
Use
of
optional
accessories
(Sold
Separately)
„25
1.
P-CS
Sénarmont
Compensator
...
....
sen
25
2.
P-CQ
Quartz
wedge
................,
.
..
26
3.
Attachable
Mechanical
Stage
....
.
НИ
27
Assembly
ee
Mana
ran
28
TroubleshootingTables...............................
.
34
[A
Care
and
Maintenance
.
.
38
Specifications
and
З4апдагас
..........................
39

Names
of
Component
Parts
and
Operational
Parts
If
the
microscope
has
not
yet
been
assembled,
see
chapter
“4.
Assembly”
first.
Binocular
eyepiece tube
Polarizing
intermediate tube
Diopter
adjustment rin
Bertrand lens
centering screw
Y
Analyzer slider
P-CL 1/44 & tint plate
A
Revolving
nosepiece
Specimen clip 0
Coo
Objective
centering
screws
Substage
Objective
Condenser
clamp
-
screw
Vernier
— |
Coarse torque adjust- OW |
ment ring (TORQUE) |
ment ring (TORQUE) |
Coarse focus knob OWN |
asr ccus no9 |
Stage rotation
clamp screw
Circular
Fine
focus
knob
OM
||
:
graduated
stage
so
TU
SER
Brightness
adjuster
ali
:
=
a
ce
Stage
clamp
screw
(LAMP)
|
O)
=
“tr
Cp
ES)
LITE
X
EX
Polarizer
IG
à
9
(Bottom
of
the
condenser)
Orientation plate
WV
Auto-photo voltage
selection switch

1.
Names
of
Component
Parts
and
Operational
Parts
Bertrand lens focus
ring
_
SU
Intermediate
tube
à
p
k
сатр
screw
Bertrand
lens
turret
ZDA
lam
screw
|
Condenser top lens
swing-out knob
Condenser aperture
scale
Coarse
focus
stopper
ring (CLAMP)
Condenser aperture
diaphragm ring
Fine focus knob
Power switch
Field lens
Field diaphragm ring
Tool
holder
Rating
label
(Holds a hexagonal screwdriver and two centering screws.)
Lamp cover
AC input connector
Power cord

Microscopic
Procedure
The
general
procedure
for
microscopy
is
described
below.
For
details
on
each
step,
refer
to
the
corresponding
item
in
chapter
“3.
Operation
of
Each
Part.”
If
the
microscope
has
not
yet
been
assembled,
see
chapter
“4.
Assembly”
first.
Turn on the power.
(Flip
the
switch
to
the
“|”
side.)
ER
Flip
the
switch
to
the
“
|"
side.
As
soon
as
the
power
comes
on,
the
switch
lights.
Set
the
brightness
adjuster
to
give
H
the
desired brightness.
:
Pressing
the
auto-photo
switch
will
set
the
voltage
for
the
brightness
that
provides
the
best
color
eproduction.
(p.21)
Insert filters NCB11 into the
optical path.
NCB11 improves color
reproduction. (p.20)
Push
in
the
analyzer
slider
of
the
intermediate
tube
to
remove
the
analyzer
from
the
optical
path.
Push in the
analyzer slider.
Put
the
Bertrand
lens
turret
in
the
“O”
position
to
remove
the
Bertrand
lens
from
the
optical
oa
|
=
-
path.
e
izi
=
CEE
—
[EI
Turn
the
turret
to
the
“O”
position.
3

Move the 10x objective into the
optical path.
Rotate the revolving nosepiece
until the 10X objective clicks into
place.
Set the specimen in place with the
cover glass facing up.
Raise the condenser as high as it
À will go.
Fully open the field diaphragm and
condenser aperture diaphragm.
2. Microscopic Procedure
B Set the 10x
/ objective.
Cover giass
facing up
Set the
specimen.
四 Fully open
= the condenser
aperture
diaphragm.
SS
| 图
Ell Raise the condenser. L
El Fully open the
field diaphragm.
+

Loosen
the
coarse
focus
stopper
ring.
Rotate
it
in
the
direction
opposite
the
arrow
on
the
base
as
far
as
it
Set
the
optical
path
to
100%
of
the
binocular
eyepiece
when
using
the
trinocular
eyepiece
tube.
(p.16)
Turn
the
diopter
adjustment
ring
of
the
right
eyepiece
(containing
the
crosshairs)
and
focus
on
the
crosshairs.
Focus
on
the
specimen.
(p.14)
9 В
— Bd Loosen the coarse
focus stopper.
+
Focus on
T
the
reticle
crosshairs.
a
N
1
\
i
1
!
1
\
f
%
é
M
之
σοι
Focus on the specimen.
%
10