Nikon ECLIPSE Ni-E User manual

Specifications
Maintenance
and Storage
TroubleshootingAssembly
Upright Motorized Microscope
Instruction Manual
Assembly/Maintenance
M571 E 11.8.NF.1 (2/2)

Introduction
i
Introduction
Thank you for purchasing a Nikon product.
This instruction manual is written for users of the Nikon ECLIPSE Ni-E microscope. To ensure correct usage, read this
manual carefully before operating this product.
●No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.
●The contents of this manual are subject to change without notice.
●The equipment described in this manual may differ from the actual product in its appearance.
●Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If
you note any points that are unclear or incorrect, please contact your nearest Nikon representative.
●Some of the equipment described in this manual may not be included in the set you have purchased.
●If you intend to use any other equipment with this product, read the manual for that equipment too.
●If this equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may
be impaired.
●Training: This product can be used without special training, provided that this manual is read thoroughly before use. Kindly
contact your nearest Nikon representative if you have any questions, find any errors, or wish to provide us with your
opinion.

Introduction
ii
Contents of the Manual
The instruction manual for ECLIPSE Ni-E is provided in two volumes.
Operation
Safety Precautions
Components
Microscopy Operations
Before Microscopy
Operation Flowchart
Bright/Dark-field Microscopy Procedure
Epi-fluorescence Microscopy Procedure
Differential Interference Contrast Microscopy
Procedure
Phase Contrast Microscopy Procedure
Individual Operations
Assembly/Maintenance (This manual)
Assembly
Troubleshooting
Maintenance and Storage
Specifications
Before reading the “Assembly/Maintenance” manual,
read the “Safety Precautions” in the “Operation”
manual.
Symbols Used in This Manual
The following symbols are used in this manual.
Symbols for Safety
WARNING
CAUTION
Highlights important information that should be noted for safety. Read “Safety Precautions” for details.
Other Symbols
Indicates information you should note or comply with to prevent defects or malfunction of this product.
Indicates information you should be aware of in using this product, as well as other useful information.

Summary of Contents
iii
Specifications
Maintenance
and Storage
TroubleshootingAssembly
Summary of Contents (See the next page for the detailed contents.)
Introduction
Contents of the Manual
Symbols Used in This Manual
Assembly
Troubleshooting
Maintenance and Storage
Specifications

Contents
iv
Contents
Introduction .........................................................................................................................i
Contents of the Manual ....................................................................................................ii
Symbols Used in This Manual ..........................................................................................ii
Summary of Contents ....................................................................................................... iii
Assembly.................................................................................................. 1
1 ECLIPSE Ni-E System Configuration........................................................................2
2 Components List .......................................................................................................4
3 Assembly Method......................................................................................................6
Introduction : Motorized Device Combination ................................................................. 6
[1] Check input voltage............................................................................................ 7
[2] Unfasten the elevating section........................................................................... 7
[3] Escape the elevating section. ............................................................................ 8
[4] Attach the motorized ND filter wheel (optional).................................................. 8
[5] Attach the DIA motorized shutter (optional). ...................................................... 8
[6] Attach the lamp. ................................................................................................. 9
[7] Attach the dia-illumination lamphouse. .............................................................. 9
[8] Attach the contact arm/standard arm. .............................................................. 10
[9] Attach the connector box (optional). ................................................................ 10
[10] Attach the epi-fluorescence cube turret and epi-fluorescence attachment
(required for epi-fluorescence microscopy).......................................................11
■Attaching the filter cube .......................................................................... 12
■Replacing excitation and barrier filters ................................................... 13
■Attaching the light shielding plate........................................................... 13
[11] Attach the motorized barrier filter wheel (optional). ......................................... 14
■Attaching the barrier filter ....................................................................... 15
[12] Attach the quadrocular tilting tube and DSC zooming port for
quadrocular tube. ............................................................................................. 16
[13] Attach the motorized excitation filter wheel (optional)...................................... 17
■Attaching the excitation filter................................................................... 18
[14] Attach the EPI motorized shutter (optional). .................................................... 18
[15] Attach eyepieces. ............................................................................................. 18
[16] Attach the substage.......................................................................................... 19
[17] Attach the stage. .............................................................................................. 20
[17.1] Rotatable ceramic coated stage...................................................... 20
[17.2] Motorized XY stage ......................................................................... 20
[18] Attach the condenser........................................................................................ 23
[18.1] Motorized universal condenser ....................................................... 23
[18.2] Manual condenser (with the condenser holder).............................. 25
■Attaching the optical module to the universal condenser....................... 25
■Attaching the DIC module to DIC condenser ......................................... 29
[19] Attach the nosepiece........................................................................................ 31
[20] Attach the objective. ......................................................................................... 31
[21] Attach the DIC rotation polarizer unit (required for differential
interference contrast microscopy). ................................................................... 31
[22] Attach the eyelevel riser (optional)................................................................... 32
[23] Attach a camera and connect the DS camera control unit (optional). ............. 32
[23.1] Attaching a camera to the DSC zooming port for
quadrocular tube ............................................................................. 32
[23.2] Installing a camera head to the DSC port for ergonomic
binocular tube.................................................................................. 33
[23.3] Installing a camera head to the quadrocular tilting tube ................. 33
[23.4] Installing a camera head to the trinocular tube ............................... 33

Contents
v
[24] Replace the ND filter ........................................................................................ 34
[25] Connect the motorized unit cable..................................................................... 34
[26] Connect the power cord. .................................................................................. 35
Troubleshooting..................................................................................... 37
1 Optical System and Operation ................................................................................38
1.1 General............................................................................................................. 38
1.2 Epi-fluorescence Microscopy ........................................................................... 42
1.3 Differential Interference Contrast Microscopy.................................................. 43
1.4 Phase Contrast Microscopy ............................................................................. 44
2 Electrical System.....................................................................................................45
2.1 General............................................................................................................. 45
2.2 Epi-fluorescence Microscopy ........................................................................... 51
Maintenance and Storage ..................................................................... 53
1 Replacing the Lamp ................................................................................................54
2 Cleaning ..................................................................................................................55
2.1 Cleaning Lenses............................................................................................... 55
2.2 Cleaning Parts Other than the Lens................................................................. 55
2.3 Cleaning Immersion Oil.................................................................................... 56
2.4 Decontaminating the Product........................................................................... 56
3 Restoring Microscope Data to Factory Default .......................................................56
4 Transportation (Using the fastening position mode switch).....................................57
5 Storage....................................................................................................................57
6 Periodic Inspections (Charged)...............................................................................58
Specifications ........................................................................................ 59
1 Microscopy (Principles) ...........................................................................................60
2 Performance Properties ..........................................................................................60
3 Physical Properties .................................................................................................63

Contents
vi

1
Assembly
1
Chapter
1
Assembly
This chapter contains the system configuration diagram and components list for Ni-E, and instructions on assembling the
system.
Read appropriate notes such as CAUTION “9 Cautions on assembling and installing the product” at the beginning of
the separately provided instruction manual “Operation” or “4 Installation location” in “Notes on Handling the Product” in the
same manual before you work on assembling.
■Assembly tools provided with the microscope
•Hex driver x3: 2 mm across flats (for all three)
•Hex wrench x2: 3 and 4 mm across flats

Chapter 1 Assembly
2
Assembly
1 ECLIPSE Ni-E System Configuration
D-FB
*1: Use with a motorized quadrocular tilting tube or motorized barrier filter wheel requires a motorized or intelligent
epi-fluorescence cube turret.
*2: Use with a motorized DSC zooming port requires a motorized quadrocular tilting tube.
Eyelevel rise
r
Analyzer unit
Epi-fluorescence
cube turret*1
Epi-fluorescence
attachment
Ergonomic tubeBinocular tube Trinocular tube
DSC port for ergonomic
binocular tube
Centering
telesco
p
e CFI eyepiece TV adapte
r
Nosepiece
with
analyzer slot
Nosepiece
Analyze
r
slider
CFI objective
ENG mount camera
C mount camera
photomicrography device DS camera head
Tube adapter for
quadrocular tube
DSC zooming port
Quadrocular
tiltin
g
tube*2
Camera cable
to DS-U3 or
DS-L3
Connected to
Ni-E with a
motorized shutter
cable
Motorized
shutte
r
Motorized
shutter
adapter
Contact arm
Lambda plate
DIC
slider
DIC
nosepiece
To N i- E mai n
body
Filte
r
cube
FL/DIC
analyzer
Excitation
balancer
To connector
box
HG precentered
fiber illuminator
Ni-E main bod
y
Motorized barrier filter wheel
Motorized
excitation filter
wheel
To c o n t r ol
box A
Motorized model:
connected to Ni-E
with RS-232C
Standard arm
Mercury lamp
HG fibe
r
HG adapte
r
Various adapters

Chapter 1 Assembly
3
Assembly
0.20.40.60.81.01.21.4
Achr-Apl N.A=1.4
Coarse
Fine
Ex Fine
XYSpeed
Constant Speed
Z Speed
Coarse
Fine
Ex Fine
+Y
-Y
-X +X
POWER
*3: USB connection is not available for DS-U3 DS camera control unit.
Ni-E main bod
y
Motorized XY stage
Condense
r
holder
Polarize
r
unit
DS-L3/DS-U3
DS camera
control unit
Motorized
shutter adapter
Motorized shutte
r
To control box A
using the
extension cable
Control
box A
Lamphouse
Connector bo
x
PC
Condense
r
Rotatable ceramic
coated stage
To motorized
XY stage
controller
using a driver
cable
Substage for rotatable
ceramic coated
stage/motorized XY stage
Connected to
connector box with
a motorized shutter
cable
To c o n ta c t
arm
Motorized
universal
condenser
Joystic
k
controlle
r
Ergo controlle
r
Motorized ND filte
r
To connector
box
Motorized XY
stage
controller
Connected to
Ni-E
with USB
cable*3
Connected to
DSC1 of Ni-E or
connector box with
camera trigger
cable

Chapter 1 Assembly
4
Assembly
2 Components List
The ECLIPSE Ni-E components are shown in the table below.
Depending on when you purchased the ECLIPSE Ni-E product, there may be products not yet available for use and
products not listed that are already available for use. Contact your nearest Nikon representative for details.
Device Device Name Model Remarks
Microscope Main
Body
ECLIPSE Ni-E
(12V 100W 100-240V external power
supply for illumination)
ECLIPSE Ni-E
Precentered lamphouse (12V 100W) NI-LH
Illuminator for
dia-illumination Halogen lamp (100W) PHILIPS 7724
OSRAM HLX 64623
Standard arm NI-SAM Combine with a manual model such as a nosepiece.
Arm Contact arm NIE-CAM Combine with a motorized models such as an intelligent or
motorized nosepiece for Ni-E.
Binocular tube C-TB C-TBM mildew-proof type also available
Trinocular tube F C-TF C-TFM mildew-proof type also available
Trinocular eyepiece tube T C-TT
Ergonomic tube C-TE2
Quadrocular tilting tube NI-TT
Tube
Motorized quadrocular tilting tube NI-TT-E
DSC port for ergonomic binocular tube C-TEP2
DSC zooming port for quadrocular tube NI-RPZ
DSC port
Motorized DSC zooming port for
quadrocular tube NI-RPZ-E
Connected to Nikon DS camera head or other C mount camera
Nikon DS camera head control is DS-L3/DS-U3.
CFI eyepiece CFI
CFI UW
Eyepiece
Centering telescope C-CT
Universal quintuple nosepiece L-NU5
BD quintuple nosepiece L-NBD5
Sextuple nosepiece C-N
Sextuple nosepiece with analyzer slot C-NA
Sextuple DIC nosepiece D-ND6
Intelligent DIC sextuple nosepiece NI-ND6-I
Septuple intelligent nosepiece NI-N7-I
Motorized sextuple DIC nosepiece NI-ND6-E
Nosepiece
Motorized septuple nosepiece NI-N7-E
Objective CFI objective
CFI
LU Plan Fluor Epi
LU Plan Fluor BD
LU Plan Fluor Epi/BD can be attached to the L-NU5 or L-NBD5
nosepiece. To attach a non-BD objective to L-NBD5, an LU
Nosepiece Adapter is required.
Substage Substage for rotatable ceramic coated
stage/motorized XY stage NI-SSR
NI-E Right Handle Rotatable Ceramic
Coated Stage with Holder NIE-CSRR2
NI-E Left Handle Rotatable Ceramic
Coated Stage with Holder NIE-CSLR2
Stage
Motorized XY stage NI-S-E
Condenser holder Condenser holder NI-CH
Dark Field Condenser (Oil) and (Dry)
C-C Aplanatic Condenser
C-C Achromat Condenser No.9
C-C Abbe Condenser No.9
X LWD Condenser
C-C Slide Achro Condenser 2-100x
C-C Achromat Swing-out Condenser
1-100x
DIC Condenser (Oil) D-CUO Used with DIC Module (Oil) mounted.
Universal Condenser (Dry) NI-CUD Supports DIC Module (Dry), PH Module, D-C Dark Field
Module, NI-CALN1 2-4x Auxiliary Lens, or D-C 2-4x Lens
Condenser
Motorized Universal Condenser (Dry) NI-CUD-E Same module as for NI-CUD, attached directly onto the
substage
Epi-fluorescence cube turret NI-FLT6
Intelligent epi-fluorescence cube turret NI-FLT6-I
Epi-fluorescence
cube turret
Motorized epi-fluorescence cube turret NI-FLT6-E
Filter Cubes and NI-FA FL/DIC Analyzer Slider attachable.

Chapter 1 Assembly
5
Assembly
Device Device Name Model Remarks
Epi-fluorescence
attachment Epi-fluorescence attachment NI-FLEI D-FB Excitation Balancer attachable
HG Precentered Fiber Illuminator C-HGFI
Mercury lamp
illuminator Motorized HG precentered fiber
illuminator C-HGFIE
Analyzer slider for DIC D-DA Can be attached to C-NA, D-ND6, NI-ND6-I, NI-N7-I, NI-ND6-E,
or NI-N7-E Nosepiece
Analyzer tube for simple polarization C-ISA Combined with C-SP Polarizer Unit
Analyzer tube for first-order red
compensation C-IA Combined with C-TP Polarizer Unit
Analyzer slider for simple polarization D-SA
Analyzer slider for first-order red
compensation C-AS
DIC slider D-C Can be attached to D-ND6, NI-ND6-I, or NI-ND6-E Nosepiece.
Analyzer unit/
Slider
Lambda plate D-LP Can be attached to D-ND6, NI-ND6-I, or NI-ND6-E Nosepiece.
DIC rotatable polarizer unit D-DP
Polarizer unit for simple polarization C-SP
Polarizer unit
Polarizer unit for first-order red
compensation C-TP
Motorized ND filter NI-ND-E Requires NIE-CB to be connected to Ni-E
Motorized excitation filter wheel NI-EXW-E Requires NIE-CB to be connected to Ni-E
Filter
Motorized barrier filter wheel NI-BAW-E
Motorized shutter NI-SH-E
To connect a second unit (on the dia-illumination side), NIE-CB
must be connected to Ni-E.
An NI-SHCS motorized shutter cable short is required for
connection.
Motorized shutter adapter for upright
epi-fl NI-SHAEP-U
Motorized shutter adapter for inverted
epi-fl TI-SHAEP-I Required when using NI-EXW-E motorized excitation filter wheel
Shutter
Motorized shutter adapter for
dia-illumination NI-SHADI
Control Box A NI-CTLA
DS-L3/DS-U3 DS camera control unit DS-L3
DS-U3
Connect DS-L3 with the Ni-E main body using an USB cable.
Connect DS-L3/DS-U3 with the Ni-E main body or a connector
box using a camera trigger cable.
Connect it to the camera using a DS camera I/F cable.
Connector box NIE-CB
Ergo controller NI-ERG Requires NIE-CB to be connected to Ni-E
Joystick controller NI-SJ Requires NIE-CB to be connected to Ni-E
Controller
Motorized XY stage controller TI-S-CON Requires NIE-CB to be connected to Ni-E
Eyelevel riser Eyelevel riser C-ER2
DSC support column NI-RPS
DSC retention
support DSC adapter A and B NI-RPSAA
NI-RPSAB
Tube adapter for
quadrocular tube Tube adapter for quadrocular tube C-TAQ
Can be attached to the quadrocular tilting tube. Connect a C
mount camera, ENG mouth camera, or a photomicrography
device via various adapters.
TV adapter TV adapter Y-T V
Y-TV55
Y-TV can be attached to C-TF or C-TT Trinocular Tube. Connect
a C mount camera, ENG mount camera, or photomicroscopy
device with an adapter.
Y-TV55 can be attached to C-TF or C-TT Trinocular Tube.
Connect a C mount camera with C mount adapter 0.55x.

Chapter 1 Assembly
6
Assembly
3 Assembly Method
Introduction : Motorized Device Combination
The available combinations are specified when installing the following four types of motorized device to Ni-E using
contact arms. If any combination not allowed is used, an error message will be displayed when the power is turned
on. Therefore, use only in specified combination.
<Target device>
•Motorized/Intelligent epi-fluorescence cube turret
•Motorized barrier filter wheel
•Motorized quadrocular tilting tube
•Motorized DSC zooming port for quadrocular tube
Available motorized device combination with contact arm
1st level 2nd level 3rd level
Contact arm
Motorized quadrocular tilting tube
with/without Motorized DSC
zooming port (at rear)
— —
Contact arm Motorized/Intelligent
epi-fluorescence cube turret 1 - (Not connected) —
Contact arm Motorized/Intelligent
epi-fluorescence cube turret 1
Motorized quadrocular tilting tube
with/without Motorized DSC
zooming port (at rear)
—
Contact arm Motorized/Intelligent
epi-fluorescence cube turret 1
Motorized/Intelligent
epi-fluorescence cube turret 2 —
Contact arm Motorized/Intelligent
epi-fluorescence cube turret 1
Motorized/Intelligent
epi-fluorescence cube turret 2
Motorized quadrocular tilting tube
with/without Motorized DSC
zooming port (at rear)
Contact arm Motorized/Intelligent
epi-fluorescence cube turret 1 Motorized barrier filter wheel —
Contact arm Motorized/Intelligent
epi-fluorescence cube turret 1 Motorized barrier filter wheel
Motorized quadrocular tilting tube
with/without Motorized DSC
zooming port (at rear)
<Error display>
(1) The message shown on the right side is displayed
on the front display panel of the Ni-E.
_*_Illegal_Connection_*
[ER3]
(2) The power LED on the front of the contact arm blinks
at 0.2 second interval. (normally ON without
blinking)
50
60
70
Power LED blinking
(3) A buzzer beeps repeatedly.

Chapter 1 Assembly
7
Assembly
Assemble the device in accordance with the following procedure.
Cables are required for some motorized devices, but assembly may be easier if cables are connected after all devices are
assembled. See “25 Connect the motorized unit cable” for the locations of all cables.
Precautions for connecting a cable
Cable connections are required for some motorized and peripheral devices.
Be sure to turn off the power to the microscope and peripheral devices before starting cable connection.
Cable connections are described in each assembly step. Nikon recommends connecting cables at the end of the
assembly (except when connecting a cable for the motorized universal condenser). See “25 Connect the motorized
unit cable.” for the connector connections.
Information setting regarding motorized models
Set information of the motorized devices or optical element attached on the microscope setup menu for the DS-L3 DS Camera
Control Unit. (See Chapter 3 “23 Operation on DS-L3” - “23.1 Setting Up the Microscope.)
1
Check input voltage.
Check the input voltage indicated on the back of control
box A. Use the microscope only if the indicated input
voltage matches the power supply voltage for the area in
which the microscope will be used.
WARNING
If the indicated voltage and the supplied voltage differ,
do not attempt to use the microscope. Contact your
nearest Nikon representative to seek advice.
Ni-E CONTACT
ARM1
LAMP
FASTENING
POSITION SW
OFF ON
100-240V~ 5A 50/60Hz
MODEL NI-CTLA
MADE IN JAPAN
This device complies with Part
15 of the FCC Rules. Operation
is subject to the following
two conditions:
(1) this device may not cause
harmful interference, and
(2) this device must accept any
interference received,
including interference that
may cause undesired
operation.
This Class A digital apparatus
complies with Canadian ICES-003.
Cet appareil numérique de la
classe A est confirme à la
norme NMB-003 du Canada.
540001
Checking the input voltage
2
Unfasten the elevating section.
Tool: flathead screwdriver
Loosen and remove the tightening screw at the front of
the elevating section.
IN
ND
8ND
32 NCB
11
OUT
Unfasten elevating section
Input voltage
label
Tightening
screw

Chapter 1 Assembly
8
Assembly
3
Escape the elevating section.
(1) Check that the power switch for control box A is OFF.
(Power LED is off.)
(2) Connect the Ni-E connector on control box A with
the BOX connector on the rear of the microscope
using a cable.
(3) Plug in the power cord to the AC inlet of the control
box A and the other end of the power cord to a wall
outlet.
(4) Turn on the power for control box A. (Power LED
turns on.)
(5) Turn the focus knob to move the elevating section to
the upper limit.
(6) Turn off the power for control box A. (Power LED
turns off.)
(7) Remove the power cord from the wall outlet.
(8) Remove the connected connection cable and power
cord from the microscope and control box A.
Ni - E CONTACT
ARM1
LAMP
FASTENING
POSITION SW
OFF ON
100-240V~ 5A 50/60Hz
MODEL NI-CTLA
MADE IN JAPAN
This device complies with Part
15 of the FCC Rules. Operation
is subject to the following
two conditions:
(1) this device may not cause
harmful interference, and
(2) this device must accept any
interference received,
including interference that
may cause undesired
operation.
This Class A digital apparatus
complies with Canadian ICES-003.
Cet appareil numérique de la
classe A est confirme à la
norme NMB-003 du Canada.
540001
Control Box A (Front/Rear)
MODEL ECLIPSE Ni-E
NIKON CORPORATION
TOKYO, JAPAN
MADE IN JAPAN
This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including interference that may cause undesired operation.
This Class A digital apparatus complies with Canadian ICES-003.
Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.
910001
CONTACT ARM2
DSC1
HGFIE
BOX
USB
EPI SHUTTER
Ni-E Microscope (Rear)
4
Attach the motorized ND filter wheel (optional).
Tool: Hex driver (2 mm across flats)
(1) Attach it to lamphouse mount at the rear of the
microscope base and tighten the fixing screws from
the top.
(2) Connect the motorized ND filter wheel cable to the
ND connector on the connector box.
IN
ND
8ND
32 NCB
11
OUT
CONTACT ARM2
USB
DSC
HGFIE
BOX
MODEL ECLIPSE Ni-E
NIKON CORPORATION
TOKYO, JAPAN
MADE IN JAPAN
Thisdevice complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1)this device may not cause harmful interference, and
(2)this device must accept any interference received, including interference that may cause undesired operation.
ThisClass A digital apparatus complies with Canadian ICES-003.
Cetappareil numérique de la classe A est confirme à la norme NMB-003 du Canada.
910001
Attaching the motorized ND filter wheel
5
Attach the DIA motorized shutter (optional).
The motorized shutter is attached by Nikon.
Contact your nearest Nikon representative when the DIA
motorized shutter needs to be mounted or removed.
BOX (Connect to Ni-E for the control box A)
Power switch
Power LED Ni-E (Connect to Ni-E BOX)
AC inlet
ND filter wheel
fixing screw

Chapter 1 Assembly
9
Assembly
6
Attach the lamp.
Tool: Hex wrench (3 mm across flats)
Lamp handling precautions
Avoid touching the glass surface of the lamp with
your bare hands.
(1) Loosen a lamphouse-cover fixing screw and lift up
the cover to remove it.
(2) Push in the lamp clamp lever to open the socket pin
hole. Attach the lamp while holding down the lever.
Put the lamp clamp lever back to its original position.
Specified lamp: PHILIPS7724
or OSRAM HLX64623
(3) Reattach the cover back to its original position and
tighten the lamphouse-cover fixing screw.
Removing the lamphouse cover
Attaching the lamp
7
Attach the dia-illumination lamphouse.
Tool: Hex driver (2 mm across flats)
(1) Insert the lamphouse at the rear of the microscope
and tighten the fixing screws.
(2) Connect the dia-illumination lamphouse cable to the
lamp connector of the control box A using a relay
cable.
IN
ND
8ND
32 NCB
11
OUT
EPI SHUTTER
CONTACT ARM2
USB
DSC
HGFIE
BOX
MODEL ECLIPSE Ni-E
NIKON CORPORATION
TOKYO, JAPAN
MADE IN JAPAN
Thisdevice complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1)this device may not cause harmful interference, and
(2)this device must accept any interference received, including interference that may cause undesired operation.
ThisClass A digital apparatus complies with Canadian ICES-003.
Cetappareil numérique de la classe A est confirme à la norme NMB-003 du Canada.
910001
1.
2.
3.
Do not touch the lamphouse while the lamp is lit.
The surface of the lamphouse becomes hot when the
lamp is on.
Turn off the power and allow the lamp and lamohouse
to cool enough before replacing the lamp.
Wait for at least 30 minutes after turning off the
lamp.
Use 12V100W HALOGEN lamp only.
HALOGEN 12V100W
952001
NI-LH
CAUTION!
-High Temperature-
JAPAN
Securing the dia-illumination lamphouse
Lamphouse
fixing screw
Fixing screw
Lamphouse
cover
Insert the hex wrench and
loosen the fixing screw.
Lamp
clamp lever
Lamp
Lamphouse
fixing screw

Chapter 1 Assembly
10
Assembly
8
Attach the contact arm/standard arm.
The attachment procedure is the same for standard arm
and contact arm. A contact arm must be attached when
using motorized accessories. The contact arm has a
contact point. Remove the contact cover before
mounting the motorized device on the arm.
Tool: Hex wrench (4 mm across flats)
(1) Place the arm while aligning it with the two
positioning pins on the main body and tighten the
fixing screws (x4).
(2) Connect the cable from the CONTACT ARM1
connector of the contact arm to the CONTACT
ARM1 connector of control box A.
(3) Connect the cable from the CONTACT ARM2
connector of the contact arm to the CONTACT
ARM2 connector of the Ni-E main body.
IN
ND
8ND
32 NCB
11
OUT
Attaching the arm
(Example of contact arm)
9
Attach the connector box (optional).
This must be attached when using the following devices:
•Motorized ND filter wheel
•Ergo controller/joystick controller
•Motorized excitation filter wheel
•Motorized XY stage controller
•DIA motorized shutter (dia-illumination side)
•DSC2 connector to camera
Tool: Hex driver (2 mm across flats)
(1) Loosen fixing screws (x3) on the connector box
mount to remove the cover.
(2) Insert the connector on the connector box into the
connector on the microscope.
(3) Fix the connector box with previously removed
screws.
IN
ND
8ND
32 NCB
11
OUT
EPI SHUTTER
CONTACT ARM2
USB
DSC
HGFIE
BOX
MODEL ECLIPSE Ni-E
NIKON CORPORATION
TOKYO, JAPAN
MADE IN JAPAN
Thisdevice complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1)this device may not cause harmful interference, and
(2)this device must accept any interference received, including interference that may cause undesired operation.
ThisClass A digital apparatus complies with Canadian ICES-003.
Cetappareil numérique de la classe A est confirme à la norme NMB-003 du Canada.
910001
1.
2.
3.
Do not touch the lamphouse while the lamp is lit.
The surface of the lamphouse becomes hot when the
lamp is on.
Turn off the power and allow the lamp and lamohouse
to cool enough before replacing the lamp.
Wait for at least 30 minutes after turning off the
lamp.
Use 12V100W HALOGEN lamp only.
HALOGEN 12V100W
952001
NI-LH
CAUTION!
-High Temperature-
JAPAN
ND
ERGO/JOY
DSC2
Attaching the connector box
Connector box
fixing screws
Positioning pins
Connecto
r
Arm fixing screw
Contact cove
r

Chapter 1 Assembly
11
Assembly
10
Attach the epi-fluorescence cube turret and epi-fluorescence attachment
(required for epi-fluorescence microscopy).
Tool: Hex driver (2 mm across flats)
Hex wrench (3 mm across flats)
First, connect the epi-fluorescence cube turret and
epi-fluorescence attachment and then attach on the
microscope arm.
(1) Insert the positioning pin of the epi-fluorescence
attachment while aligning it with the positioning hole
on the epi-fluorescence cube turret side, and then
tighten the connection fixing screws (x2) on the
epi-fluorescence cube turret with a hex driver.
(2) When motorized or intelligent epi-fluorescence cube
turret is used, remove the contact cover of the
contact arm.
(When the manual epi-fluorescence cube turret is
used, do not remove the contact cover.)
(3) Loosen the fixing screw on the front of the arm using
a hex driver so that the tip of the screw does not
protrude into the connecting section.
(4) Engage the round dovetail and convex contact on
the bottom of the epi-fluorescence cube turret with
the round dovetail and concave contact of the arm,
and then slide the entire mounted units to the rear
side.
(The manual epi-fluorescence cube turret has no
convex contact point.)
(5) Tighten the fixing screw loosened in step (3).
(6) Tighten the fixing screws (x4) on the top of the
epi-fluorescence attachment using a hex wrench.
(7) Attach the HG adapter to the bayonet mount at the
rear of the epi-fluorescence attachment and connect
the HG precentered fiber illuminator. (See instruction
manual provided with the HG precentered fiber
illuminator.)
F. STO P A. STOP
EX.ADJ
Connecting epi-fluorescence cube turret to
epi-fluorescence attachment
F. ST OP A. STOP
EX. ADJ
2
3
4
5
6
Attaching and fixing epi-fluorescence cube turret
and epi-fluorescence attachment set
EPI SHUTTER
CONTACT ARM2
ND
ERGO/JOY
DSC2
F.STOP A. STOP
EX. ADJ
Attaching the HG adapter
Epi-fluorescence
attachment fixing
screw
Positioning
hole
Bayonet mount
Connection fixing screw
Epi-fluorescence
cube turret
Epi-fluorescence
attachment
Contact cove
r
Fixing screw
HG fibe
r
HG adapte
r

Chapter 1 Assembly
12
Assembly
■Attaching the filter cube
Precautions for attaching and removing the cube
•Be sure to check that the light source is turned off before attaching or removing the cube.
•Be sure to check that the power switch for control box A is turned off and then attach the cube by rotating the
cube switchover turret. Also for the motorized epi-fluorescence cube turret, attach it by manually rotating the
inside turret.
(1) Pull out the filter cube replacement cover on the
front of the epi-fluorescence cube turret to remove it.
(2) Attach the filter cube to the slot.
(3) Insert the filter cube nameplate into the slot cover
window.
Insert into the same address window as the address
shown inside the slot.
5
6
1
A
λ
5
4
3
2
1
ND4
ND8
ND16
50
60
70
A
5
UV-2A
EX 330-380
DM 400
BA 420
(4) Turn the cube switchover turret to attach the filter
cube in the remaining slots and also insert the
nameplate.
For bright-field microscopy
For bright-field microscopy, be sure that address 1 is
empty.
For motorized/intelligent epi-fluorescence cube
turret, only address 1 can be set to [OPEN].
(5) Restore the slot cover back to its original position.
A
Attaching the filter cube
Filter cube
replacement slot cover
Filte
r
cube
A
ddress
indication
Nameplate
window
A
ddress
indication