Nikon ECLIPSE E600 POL User manual

Polarizing Microscope
ECLIPSE E600 POL
Instructions

I
Thank you for purchasing the Nikon products.
This instruction manual is written for the users
of the Nikon Polarizing Microscope
ECLIPSE E600 POL.
To ensure correct usage. read this manual carefully before operating the instrument.
• It is prohibited to reproduce or transmit this manual in part or whole without Nikon·s
expressed permission.
• The contents
of this manual are subject to change without notice.
• Some
of the products described in this manual may not be included in the set you
have purchased.
• Although every effort has been made to ensure the accuracy
of this manual. if you
note any points that are unclear
or incorrect. contact your nearest Nikon
representative.
Warning/Caution Symbols Used in This Manual
Although Nikon products are designed to provide you with the utmost safety during
use. incorrect usage
or disregard of the instructions can cause personal injury or
property damage. For your safety. read the instruction manual carefully and thoroughly
before using the instrument. Do not discard this manual but keep
it near the product for
easy reference.
In this manual. safety instructions are indicated with the symbols shown below. Be
sure
to follow the instructions indicated with these symbols to ensure correct and safe
operation.
Symbol
&WARNING
&CAUTION
Meaning
Disregarding instructions marked with this symbol may lead
to death or serious injury.
Disregarding instructions marked with this symbol may lead
to injury or property damage.
Meaning of Symbols Used on the Equipment
Symbol Meaning
Caution for heat.
This marking near the lamphouse calls your attention on the
following:
• Lamphouse becomes very hot during and immediately
after the illumination.
• Risk
of burns. Do not touch the lamphouse during and
immediately after the illumination.
• Make sure that the lamphouse
is sufficiently cool before
the lamp replacement.

&WARNING
2
1. Intended product use
This microscope should only be used for microscopic observation. Do not use it for any
other purpose.
2. Do not disassemble
Disassembling may cause malfunction and/or electrical shock. Do not disassemhle any
pans other than those mentioned in this manual. If you notice any malfunction. notify your
nearest Nikon representative.
3. Input voltage
Make sure that the input voltage indicated on
the back panel
of the microscope is the same
as your regional voltage.
If not the same. do
not use the microscope; instead. notify your
nearest Nikon representative immediately.
If
the microscope is used with the wrong input
voltage. a short circuit or fire may result.
causing the damage
of the microscope.
4. Power cord
To prevent electrical shock. always turn off
the power switch (flip it to the" 0" side)
before connecting or disconnecting the power
cord. Use only the following power supply
cord set. Using the wrong power cord could
result
in damage or fire.
• For I
OO- I20V area
UL Listed. detachable cord set. 3 conductor
grounding type SVT, No.
18 AWG rated at
125V, 7A minimum.
Nllton
MODEL ECLIPSE E600 POL
430001
In case of using the extension cord. use only the power supply cord with the PE (protective
earth) wire.
• For 220-240V area
3 pole power supply cord set. which must be approved according to EU/EN standards. .
Class I equipment should be connected to PE (protective earth) terminal.
In case of using the extension cord. use only the power supply cord with the PE (protective
earth) wire.
5. Heat from the light source
The lamp and the lamphouse become
extremely hot. To avoid burns. do not touch
the lamphouse while the lamp
is lit or for
thirty minutes after it has been turned off.
Furthermore.
in order to avoid the risk of fire.
do not place fabric, paper or highly
flammable materials such as gasoline,
petroleum benzine. paint thinner or alcohol
near the lamphouse while it is lit or for about
thirty minutes after it has been turned off.
The back
of the microscope also becomes hot
during use. Although this is not a
malfunction. be careful not to touch the back
of the microscope when it is hot.

&CAUTION
1. Only use the specified halogen lamp
The power supply unit in the microscope provides the power for the halogen lamp that
serves as the microscope light source. This unit can supply
power for a halogen lamp of up
to 12V-IOOW.
The specified lamp and lamphouse must be used. otherwise. the microscope
could be damaged.
Specified lamphouse (for diascopic use):
Nikon
C-LP HALOGEN 12VIOOW model
Specified lamphouse (for episcopic use):
Nikon LHS-HIOOP-2
HALOGEN 12VIOOW model
Specified lamp (for both diascopic and episcopic uses):
I2V- I
OOW LONG LIFE halogen lamp (OSRAM HLXM62.~ or
PHILIPS 7724l
2. Precautions for replacing the lamp
To prevent burns. allow the lamp to cool for at least thirty minutes after turning off the
power switch before replacing it. Furthermore. to prevent electrical shock. and damage
to
the microscope. always turn off the power switch (flip it to the·· ::_: ·· side) and unplug the
power cord before connecting
or disconnecting the lamphouse.
After replacing the lamp. he sure to attach the lamphouse cover securely. Never use the
lamphouse without its cover.
3. Do not wet the microscope
If the microscope gets wet. a short circuit may result that could damage it or make it
extremely hot. If you accidentally spill a liquid on the microscope. immediately turn off
the power switch (flip it to the" 0" side) and unplug the power cord. Then use a dry
cloth to wipe away the moisture.
If any liquid gets inside the microscope. do not use it:
instead. notify your nearest Nikon representative.
4. Weak electromagnetic waves
This microscope emits weak electromagnetic waves. The accuracy of any precision
electronic equipment may he adversely affected
if positioned too close. If the microscope
affects
TV or radio reception. move the radio or TV further away from the microscope.
5. Precautions for installation
Be careful not to pinch your hands or fingers when installing the microscope.
This microscope
is a precision optical instrument. and using or storing it under unsuitable
conditions may damage
it or may have an adverse effect on its accuracy. See "Installation
location" on
p. 32 and always use the microscope in a suitable environment.
6. Precautions for moving the microscope
First detach the lamphouse. and then securely
hold the microscope
hy the hase of the arm
from the back.
Do not grasp the focus knobs. eyepiece tube.
stage. etc. when carrying the microscope.
This could cause the part to come off.
resulling in damage to the microscope.
This microscope is a precision optical
instrument. so handle it carefully and
do not
subject it to a strong physical shock. (The
accuracy
of the ohjective in particular may be
adversely affected
by even a weak physical
shock.)
3

CONTENTS
Warning/Caution Symbols Used in This Manual ......................................................... 1
& WARNING ................................................................................................................... 2
& CAUTION .................................................................................................................... 3
D Names of Component Parts and Operational Parts ............................................. 6
Iii Microscopic Procedure ........................................................................................... 8
D Diascopic Illumination Microscopy .................................................................................. 8
El Episcopic Illumination Microscopy ................................................................................. I3
II Orthoscopic Observation/Conoscopic Observation ........................................................ I 7
D Operation of Each Part ......................................................................................... 18
D Coarse and fine focus knobs ..... .. .... .. ......... .. ........................... .. .......... .. .. .. ......... .. .. . .. . .. .... 18
I. Rotation of the coarse and fine focus knobs and vertical movement of the stage .... 18
2. Adjusting the torque of the coarse focus knob ........................................................... 18
3. Coarse focus stopper................................................................................................... 19
El Trinocular eyepiece tube .................................................................................................. 20
1. Optical path selection ................................................................................................. 20
2. Vertical tube adapter................................................................................................... 20
3. Optical path selection lever clicking ..........................................................................
20
II Diopter adjustment ........................................................................................................... 21
D Interpupillary distance adjustment ................................................................................... 21
II Condenser......................................................................................................................... 22
1. Focusing and centering ............................................................................................... 22
2. Condenser aperture diaphragm ................................................................................... 23
3. Swing-out the top lens
of the condenser .................................................................... 24
Iii Field diaphragm ............................................................................................................... 24
I.I Filters................................................................................................................................ 24
Iii Auto-photo switch (for photomicrography) .................................................................... 25
Iii Oil-immersion operation (using an oil-immersion type of objective and condenser) .... 25
mJ Orientation of polarizing plates (Polarizer and Analyzer) .........................................•.... 26
mFocusing and centering the Benrand lens........................................................................ 28
18 P-CL l/4A. & tint plate ..................................................................................................... 28
Ill Centering the objectives ................................................................................................... 28
ID Circular graduated stage for polarizing microscope ........................................................ 29
ml Use of optional accessories (Sold Separately)................................................................. 29
1. P-CS Senarmont Compensator ................................................................................... 29
'"' P-CQ Quartz wedge .................................................................................................... 30
3. Attachable Mechanical Stage ..................................................................................... 31
III Assembly ............................................................................................................... 32
~Troubleshooting Tables ........................................................................................ 41
nCare and Maintenance .......................................................................................... 45
H Specifications and Standards .............................................................................. 46
5

Names of Component Parts
and Operational Parts
If the microscope has not yet been assembled. see chapter ..4. Assembly'" first.
Polarizing
intermediate tube
Bertrand lens
centering screw
45° click-stop lever
screw
Coarse torque adjust-
ment ring (TORQUE)
Coarse focus knob
Fine focus knob
Brightness adjuster
(LAMP)
Auto-photo switch
Auto-photo voltage
selection switch
6
Photomicrographic
vertical tube adapter
Trinocular
eyepiecetube
Eyepiece
Diopter
adjustment ring
Analyzer slider
P-CL 1 /4'A & tint plate
Revolving nosepiece
Objective centering screws
Objective
Stage rotation clamp screw
Vernier
Specimen clip
Circular graduated stage
Dia-polarizer
(Inside lhe bottom of !he condenser)

Bertrand lens turret
Bertrand lens focus
ring
Condenser top lens
swing-out knob
Condenser aperture
scale
Condenser aperture
diaphragm ring
Swing-out condenser
Condenser centering
screws
Field lens
Field diaphragm ring
Epi/Dia changeover switch
Tool holder
(Holds a hexagonal screWdnver.
a hexagonal wrench and two centenng screws.)
AC input connector
Power cord
1. Names of Component Parts and Operational Parts 1'
(Rear)
Photomicrographic
equipment clamp screw
Optical path
selection lever
Eyepiece tube
clamp screw
Intermediate tube
clamp screw
Revolving nosepiece
clamp screw
Substage clamp
scr~vv
Filter release lever
Diffuser insertion/
removal screw
Epi-illuminator connector
Rating label
Lamphouse
7

8
Microscopic Procedure
The general procedure for microscopy is described below.
For details on each step. refer to the corresponding item
in chapter .. 3. Operation of Each Part."
If the microscope has not yet been assembled. see chapter "4. Assembly .. first.
1 Diascopic Illumination Microscopy
DTurn the Epi/Dia changeover
switch on the back
of the
microscope stand to the DIA
position.
a Turn on the power.
Kii:I (Flip the switch to the .. I •. side.)
As soon
as the power comes on.
the switch lights.
1:'11 Set the brightness adjuster to
a give the desired brightness.
Pressing the auto-photo switch
will set the voltage for the
brightness that provides the best
color reproduction. (p.25)
B Insert filters ND32 or 8 and
U NCB 11 into the optical path.
ND32 or 8 reduces glare in the
binocular eyepiece. NCB
11
improves color reproduction.
(p.24)
a Push in the analyzer slider of the
a intermediate tube to remove the
~ivi:v, analyzer from the optical path.
··~· i'·.·.;.··~;.
;.:i~j:
1'!!9 Put the Bertrand lens turret in the
a .. 0 .. position to remove the
Bertrand lens from the optical
path.
II Insert filters.
,_,~
II Adjust the brightness. ~ ·~,~
Auto-photo switch can be pressed _!
1-11 Push in the
, analyzer
/
~' -1'1 Turn the turret to
the "O" position.
slider.

n Move the !OX objective into the
.. optical path.
Rotate the revolving nosepiece
,,, until the !Ox objective clicks
into place.
n Set the specimen in place with
... the cover glass facing up.
Raise the condenser as high as
it
will go.
rr.i. Fully open the field diaphragm
~ and condenser apenure
diaphragm.
II Set the
2. Microscopic ProcedureI
-D Set the 10x
objective.
---
~ U!J Fully open the
field diaphragm.
9

10
m
~oosen the coarse focus stopper
nng.
Rotate it in the direction opposite
the arrow on the base as far as
it
will go. (p.19)
m Set t~e optical path to I 00</t of
lliii:ll the bmocular eyepiece when
· · using the trinocular eyepiece
tube. (p.20)
m Turn the diopter adjustment ring
11iii1 of the right eyepiece (containing
the crosshairs) and focus on the
crosshairs.
Focus on the specimen. (p.18)
I
I I
,I i $]
mLoosen the coarse focus stopper.
!:
DJ Focus on the
reticle crosshairs.
Im Set the optical path to
100% of the binocular
eyepiece.
ID Focu~ on the specimen.

. f the left
m Adjust the diopter o
lli:il eyepiece. (p.2 I)
· terpupillary
1!P-9 Adjust the m
ll:il distance. (P-
21
)
. . (p 28)
th
e objecuve. ·
mCenter
the condenser.
m Focus and center
.... (p.22)
\ /
v .
ID Center the objective.
· p cedure I
2. Microscopic ro
_ Im Adjust the
diopter nng.
Im Adjust the interpupillary
distance.
h ondenser.
Im Center t e c =-------
11

12
m Switch to any desired objective
l&;I and view the specimen.
j,.. Rotate the revolving nosepiece to
its click-stop position.
In the case of using an oil-
immersion objective. apply oil
while being careful that air
bubbles do not form between the
end
of the objective and the
specimen.
Manipulate the coarse focus
knob or fine focus knob to re-
focus
on the specimen.
Manipulate the ND filter
insertion lever to adjust the
brightness.
(When adjusting the lamp
brightness.
turn off the auto-
photo switch and manipulate the
brightness adjuster.)
Close the field diaphragm so that
it is just outside the field of view.
(p.24)
Close the condenser aperture
diaphragm to about 70 -80%
of
the numerical aperture of the
objective. (p.23)
BJ Switch the objective.
mAdjust the
brightness
with ND filters.
Sil Readjust the focus.
Pa Adjust the field diaphragm.
Pa Adjust the condenser
aperture diaphragm.

2
2. Microscopic ProcedureI
Episcopic Illumination Microscopy
D
~ ~ ..
B
EJ
II
g
m
Push in the illumination
changeover lever
of the Epi-
illuminator to the limit
(indication: B.F) to set the
brightfield illumination.
Turn the Epi/Dia changeover
switch on the back
of the
microscope stand to the EPI
position.
Turn
on the power. (Flip the
switch
to the·· I" side.)
As soon as the power comes on.
the switch lights.
Set the brightness adjuster to
give the desired brightness.
Pressing the auto-photo switch
will set the voltage for the
brightness that provides the best
color reproduction.
Insert filters ND2 or 4 and
NCB
11 of the Epi-illuminator
into the optical path.
ND2 or 4 reduces glare in the
binocular eyepiece. NCB
11
improves color reproduction.
Confirm that the ND8 anti-glare
filter lever is pushed in.
Push
in the analyzer slider of the
intermediate tube to remove the
analyzer from the optical path.
Put the Bertrand lens turret
in the
"O'' position to remove the
Bertrand lens from the optical
path.
D Set the brightfield illumination.
•• .\=' lij ~ f: -1 _Jj)_J
((_"°'+ii~
~:---- ----~ fJ Flip the switch
" ~oc::_l to the "EPI" side.
II Adjust the
brightness.
0i't
-,
1
11 Flip the switch
to
the .. I " side.
...-
'C. I
:"'~
'~
,-61 Push in the
analyzer slider.
-Iii Push in the NOS
filter lever.
-l!I Turn the turret to the "O" position.
13

14
n Move the lOX objective into the
a optical path.
" ,,, · Rotate the revolving nosepiece
~· until the I Ox objective clicks
:=.~ into place.
!l:'t Set the specimen in place with
IA:. the cover glass facing up.
m
Fully open the field diaphragm
and aperture diaphragm
of the
Epi-illuminator.
m ~oosen the coarse focus stopper
lrii:I rmg.
·· Rotate it
in the direction opposite
the arrow
on the base as far as it
will go.
!1:1 Set the optical path to I00% of
1£.1 the binocular eyepiece when
~~, ~::~ the trinocular eyepiece
Im Set the
specimen.
- II Set the 1 Ox objective.
'i:
I,
11
--
I I'~~
......

Turn the diopter adjustment ring
of the right eyepiece (containing
the crosshairs) and focus on the
crosshairs.
IE Focus on the specimen.
Adjust the diopter
of the left
eyepiece.
~ Adjust the interpupillary
Ill distance.
m Center the field diaphragm of the
11:.i Epi-illuminator.
Im Center the objective.
2. Microscopic ProcedureI
eJ Focus on the
reticle crosshairs.
ID Adjust the inter- .
pupillary distance.
Im Focus on the specimen.
IIiJ Center the
objective.
111J Center the field
diaphragm.
,.----
r ~--
I
15

16
~.:i4;~~
f,:h4r~.
0\j:}tf'.
Switch to any desired objective
and view the specimen.
Rotate the revolving nosepiece to
its click-stop position.
In the case
of using an oil-
immersion objective, apply oil
while being careful that air
bubbles do not form between the
end
of the objective and the
specimen.
Manipulate the coarse focus
knob or fine focus knob
to re-
focus on the specimen.
Adjust the brightness using the
ND filter
of the Epi-illuminator.
(When adjusting the lamp
brightness, turn
off the auto-
photo switch and manipulate the
brightness adjuster.)
Adjust the field and aperture
diaphragms
of the Epi-
illuminator.
Field diaphragm:
Close it to the size just
outside the field
of view.
Aperture diaphragm:
Close
it to the size about
70 -
809C of the numerical
aperture
of the objective.
ml Switch the objective.
fD Readjust the focus.
~f!J Adjust the field diaphragm.
:! ,f!] Adjust the condenser
I aperture diaphragm.
This completes the procedure for microscopy for normal observation. Refer to the following
section 3 for details regarding the microscopy procedures for "Orthoscopic Observation" and
"Conoscopic Observation".

2. Microscopic ProcedureI
3 Orthoscopic Observation/Conoscopic Observation
The following provides an explanation of the characteristic observation method of polarizing microscope~
along with the microscopy procedure. If normal microscopy has not yet been completed. refer to the
previous section I
or 2 and complete normal microscopy.
Orthoscopic Observation
• In this method. the specimen is observed with the
polarizer and analyzer placed
in the optical path.
•Operation
Pull out the analyzer slider and move the analyzer
into the optical path.
• In this case. the shape
of the specimen is visible
(direction
of optical axis) and the optical properties
relative to the direction
of the thickness of the
specimen can be observed.
Conoscopic Observation
• In this method. in addition to the polarizer and
analyzer. the Bertrand lens is also moved into the
optical path when observing a specimen.
•Operation
Move the analyzer into the optical path. and place the
Bertrand lens turret
of the intermediate tube in
position "B" to move the Bertrand lens into the
optical path. (Refer to p.28 for details regarding the
focusing procedure.) Place the P-CL
1/4/.. & tint
plate in the hollow position. Use
an objective having
a large numerical aperture (high magnification:
normally
40 or higher).
Analyzer in the optical path
[]]
Analyzer and Bertrand lens
in the optical path
• Specimens can be observed from various angles with diascopic light in the form of a single image.
Differing from orthoscopic observation. however. the shape
of the specimen itself is not visible
with this observation.
The settings
of each part in orthoscopic microscopy and conoscopic microscopy are summarized below.
Orthoscopic Observation Conoscopic Observation
Purpose of
To investigate the vibration direction and birefringence
To distinguish between umax1al and biaxial
observation
of observation light by observing the extinction and properties and observe the optic-axial angle
interference color due to the specimen. and optical characteristics
in minerals.
Top lens
of
1Ox or higher IN
the condenser
4x or lower OUT
IN
Benrand lens
OUT <Turret position: 0)
IN ITurret position: Bl
Aperture 1Ox
or higher 70 -80'k
of the numerical aperture Circumscribed the circumference
of the
of the objective
diaphragm
4x or lower Fully opened conoscopic field
of view (or fully opened)
Field 1Ox
or higher Circumscribed the circumference
of
Circumscribed the circumference of the
the eyepiece field
of view
diaphragm 4X
or lower Fully opened orthoscopic field
of view
17

18
Operation of Each Part
DCoarse and fine focus knobs
,;:d~
: • 1. Rotation of the coarse and fine focus knobs and
vertical movement of the stage
•••••••••••••••••
Rotating the fine focus knob one step moves the stage
1µm.
Rotating the fine focus knob one complete turn moves
the stage
0.1 mm.
Rotating the coarse focus knob one complete turn moves
the stage
12 mm.
The coarse/fine focus stroke (range
of vertical motion)
for the stage
is 2 mm up and 23 mm down from the
reference (focused) position.
Never attempt either
of the following actions. These
actions will damage the microscope.
• Rotating the left and right knobs
in opposite
directions at the same time.
• Continuing to rotate the coarse focus knob after the
stage has reached the limit
of its motion.
Adjusting the torque of the coarse focus knob
It is possible to adjust the torque of the coarse focus
knob.
To increase the torque. turn the torque adjustment ring
(TORQUE) located behind the coarse focus knob
in the
direction
of the arrow on the microscope base (i.e .. the
counter-clockwise direction). To reduce the torque. turn
the ring
in the direction opposite to the arrow (i.e .. the
clockwise direction).
i !:
Direction of coarse/fine focus knobs
and stage vertical motion

3. Operation of Each PartI
• 3. Coarse focus stopper
Clamp the coarse
focus stopper
Purpose of the coarse focus stopper
The coarse focus stopper marks the stage position at which the specimen is in focus by
restricting the movement of the coarse focus knob. (Movement of the stage by the fine focus
knob
is not restricted.)
Once the coarse focus stopper has been clamped
in position. the coarse focus knob cannot be
used to move the stage any higher.
In effect. once the coarse focus knob has been clamped in
place at the focus position. a rough focus can be attained the next time simply by turning the
coarse focus knob as far as
it will go. This feature is convenient when viewing similar
specimens one after another.
Using the coarse focus stopper
With the specimen in focus. turn the coarse focus stopper ring as far as it will go in the direction
of the arrow on the base of the microscope (about 3/4 revolution). The coarse focus stopper is
now clamped in position.
When changing the specimen. lower the stage
by turning only the coarse focus knob.
After changing the specimen, gently raise the stage by turning only the coarse focus knob as far
as it will go.
The specimen should be roughly
in focus when the stage has been raised as far as it will go: use
the fine focus knob
to bring the specimen into perfect focus.
If the coarse focus stopper is not being used, be sure to turn the coarse focus stopper ring in the
direction opposite
to the arrow on the microscope base as far as it will go.
19

20
El Trinocular eyepiece tube
~?fj 1. Optical path selection
The optical path selection lever can be used to select the way to divide the amount of light
between the binocular part and the vertical tube.
·.< ·'·
>'.:!':/'"·
Lever
position
I
::?::
:I
'.<··: ~-.:· ':
U~· 2. Vertical tube adapter
A photomicrographic vertical tube adapter is provided as
standard equipment that allows a photomicrographic
equipment to
be installed. To install the adapter. insert
it into the vertical tube and clamp three screws with the
provided screwdriver. Replace this adapter with the
optional TV vertical tube adapter when using a TV
camera.
~:jj 3. Optical path selection lever clicking
There is a switch identified by "NO CLICK" on the
bottom surface
of the eyepiece tube. Tum the switch in
the direction of the arrow with the hexagonal
screwdriver provided to disengage the clicking action
of
the optical path selection lever. Disengaging the
clicking action minimizes small vibrations produced
by
operating the lever.
Light proportion
binocular vertical
part tube
100 0
20
0
80
100