Nikon Eclipse E600 User manual

Nikon
Microscope
ECLIPSE E600
Instructions

Thank you for purchasing the Nikon products.
This instruction manual is written for the users of the Nikon Microscope ECLIPSE E600.
To ensure correct usage, read this manual carefully before operating the instrument.
* It is prohibited to reproduce or transmit this manual in part or whole without Nikon's ex-
pressed permission.
* The contents of this manual are subject to change without notice.
+ Although every effort has been made to ensure the accuracy of this manual, if you note any
points that are unclear or incorrect, contact your nearest Nikon representative.
Warning/Caution Symbols Used in This Manual
Although Nikon products are designed to provide you with the utmost safety during use,
incorrect usage or disregard of the instructions can cause personal injury or property damage.
For your safety, read the instruction manual carefully and thoroughly before using the instru-
ment. Do not discard this manual but keep it near the product for easy reference.
In this manual, safety instructions are indicated with the symbols shown below. Be sure to
follow the instructions indicated with these symbols to ensure correct and safe operation.
Symbol Meaning
AN
ARNING
Disregarding
instructions
marked
with
this
symbol
may
lead
to
death
or
serious
injury.
CAUTION Disregarding instructions marked with this symbol may lead to injury
/ \ or property damage.

Anne
1.
Intended product use
This microscope should only be used for microscopic observation. Do not use it for any other purpose.
Do not disassemble
Disassembling may cause malfunction and/or electrical shock. Do not disassemble any paris other
than those mentioned in this manual. If you notice any malfunction, notify your nearest Nikon
representative.
Input voltage
Make sure that the input voltage indicated on the
back panel of the microscope is the same as your
regional voltage. If not the same, do not use the
microscope; instead, notify your nearest Nikon
representative immediately. If the microscope is
used with the wrong input voltage, a short circuit
or fire may result, causing the damage of the
microscope.
Power cord
To prevent electrical shock, always turn off the
power switch (flip it to the “ *" side) before
connecting or disconnecting the power cord. Use
only the following power supply cord set. Using
the wrong power cord could result in damage or
fire.
* For 100-120V area
UL Listed, detachable cord set, 3 conductor
grounding type SVT, No. 18 AWG rated at
125V,7A minimum.
In case of using the extension cord, use only
the power supply cord with the PE (protective
earth) wire.
+ For 220- 240V area
3 pole power supply cord set, which must be
approved according to EU/EN standards.
Class I equipment should be connected to PE
(protective earth) terminal.
In case of using the extension cord, use only
the power supply cord with the PE (protective
earth) wire.
Heat from the light source
The lamp and the lamphouse become extremely
hot. To avoid burns, do not touch the lamphouse
while the lamp is lit or for thirty minutes after it
has been turned off.
Furthermore, in order to avoid the risk of fire, do
not place fabric, paper or highly flammable
materials such as gasoline, petroleum benzine,
paint thinner or alcohol near the lamphouse while
it is lit or for about thirty minutes after it has been
turned off.
The back of the microscope also becomes hot
during use. Although this is not a malfunction,
be careful not to touch the back of the microscope
when it is hot.
Nikon
MODEL ECLIPSE E600
120V~
24A
50/60HZ
MADE IN JAPAN

[Nana
1.
Only use the specified halogen lamp
The power supply unit in the microscope provides the power for the halogen lamp that serves as the
microscope light source. This unit can supply power for a halogen lamp of up to 12 V-100 W. The
specified lamp and lamphouse must be used, otherwise, the microscope could be damaged.
Specified lamphouse: Nikon C-LP HALOGEN 12V100W model
Specified lamp: 12V-100W LONGLIFE halogen lamp (OSRAM HLX64623 or PHILIPS
7724)
Precautions for replacing the lamp
To prevent burns, allow the lamp to cool for at least thirty minutes after turning off the power switch
before replacing it. Furthermore, to prevent electrical shock and damage to the microscope, always
turn off the power switch (flip it to the “ > side) and unplug the power cord before connecting or
disconnecting the lamphouse.
After replacing the lamp, be sure to attach the lamphouse cover securely. Never use the lamphouse
without its cover.
Precautions for operating the stage
The stage rack protrudes when the stage is moved.
When operating the focus knobs or condenser
focus knob, be careful not to graze your hand on
the end of the rack.
Do not wet the microscope
If the microscope gets wet, a short circuit may result that could damage it or make it extremely hot.
If you accidentally spill a liquid on the microscope, immediately turn off the power switch (flip it to
the “ +” side) and unplug the power cord. Then use a dry cloth to wipe away the moisture. If any
liquid gets inside the microscope, do not use it; instead, notify your nearest Nikon representative.
Weak electromagnetic waves
This microscope emits weak electromagnetic waves. The accuracy of any precision electronic
equipment may be adversely affected if positioned too close. If the microscope affects TV or radio
reception, move the radio or TV further away from the microscope.
Precautions for installation
Be careful not to pinch your hands or fingers when installing the microscope.
This microscope is a precision optical instrument, and using or storing it under unsuitable conditions
may damage it or may have an adverse effect on its accuracy. See “Installation location” on p. 24 and
always use the microscope in a suitable environment.

A cures
7. Precautions for moving the microscope
First detach the lamphouse, and then securely hold
the microscope by the base of the arm from the
back.
This microscope is a precision optical instrument,
so handle it carefully and do not subject it to a
strong physical shock. (The accuracy of the
objective in particular may be adversely affected
by even a weak physical shock.)
In addition, do not grasp the focus knobs, eyepiece
tube, stage, etc. when carrying the microscope.
This could cause the part to come off, resulting
in damage to the microscope.

CONTENTS
Warning/Caution Symbols Used in This Manual ezine see ieenionionia 1
PAN SA 2
LS CAUTION ccoo errores 3
il Names of Component Parts and Operational PartS...........................-srerrercenerienicane: 6
H Microscobp ツ tirrenia rioni rien rioniie nie iene 페미 8
El OperationofEachPart.............................................. 0.0000 aneen aaa enne oro rro riores 13
Coarse and fine focus knobs ii 13
1. Rotation of the coarse and fine focus knobs and
vertical movement of the stage .................. i 13
2. Adjusting the torque of the coarse focus knob nn... 13
3. Coarse focus stoDDeT e eee 14
Eyepiece tube optical path selection (T eyepiece tube, F eyepiece tube) ............... 15
1. Vertical tube adapter i 15
2. TandF eyepiece tubes lever clickind 上 … せ ドー トー タート ーー 15
Diopter adjustment en 16
EJ Interpupillary distance adjustment nn 16
Condenser μμ ----,,,, 17
1. Focusing and Centering ss e Pen 17
2. Condenser aperture diaphragm eee nene een ena 0 18
3. Objectiveandceondensercombinations.................................... 19
E Field diaphragm nın 19
Filters... ИИНИ 20
EJ] Stage ОА 20
1. Adjusting the torgue ofthestagemotioncontrolknobs..................................... 20
2. Stage rotation............... issues K K RKK 21
E] Auto-photo switch (for photomicrography) ーーoooeeeeeeeeeww 21
Oil-immersion operation ............. sise 22
Water-immersion operation sers 23
ASSEMDIY ............e renne nie rerian reni can aenienera nec eee nie nieonanienisanienice seneca niaenicniene zen eve nienienina 24
1. Confirming the input voltage eee eee nn nee nen n tn 25
2. Lamp and lamphouse installation and removal (lamp replacement) ................... 26
NS EA 27
4. Revolving nosepiece assembly nr nn na nan once 28
5. Eyepiece tube assembly ii 28
6. Connecting the power cord... si 1.11 29
7. Installing separately sold accessories i 29
Troubleshooting Tables .…........................................ nn n Kne 30
Viewing and control Systems .ps 30
Electrical system see 33
[3 Care and Maintenance μμ μμ” ---ϱ------ϱϱ-ϱ--ρ--- 34
Electrical Specifications osse serene seeren renee En Eee renerne 35
ASS 36

Names of Component Parts
and Operational Parts
If the microscope has not yet been assembled, see chapter “4. Assembly” first.
Photomicrographic
vertical tube adapter
Trinocular eyepiece tube
Diopter adjustment ring
Eyepiece
Specimen holder (AS. |
Condenser clamp ロン タジク Q (O
screw SS AS
—— > >
Condenser focus knob + Revolving nosepiece
Coarse torque adjustment
ring (TORQUE) Objective
Coarse focus knob
Fine focus knob N J
X DN
N | Stage
Brightness adjuster DN Stage rotation
(LAMP) clamp screw
|
Auto-photo switch AT |
Auto-photo voltage
selection switch

1. Names of Component Parts and Operational Parts
Condenser
aperture scale
Condenser aperture
diaphragm ring
Condenser
Photomicrographic
equipment clamp screw
selection lever
cm
Eyepiece tube
clamp screw
Revolving nosepiece
clamp screw
Substage clamp screw
Filter release lever
7 > Filter insertion lever
Y-axis stage motion
control knob
Fine focus knob
Field diaphragm ring
TŽ TT
a Power switch
Field lens
X-axis stage motion
a control knob
Coarse focus stopper
ring (CLAMP)
=
|
AA
ON
X
Curse
Nikon
6600
Diffuser insertion/
removal screw
Tool holder
Rating label
Lamphouse
AC input connector
Power cord

Microscopy
The general procedure for microscopy is described below.
For details on each step, refer to the corresponding item in chapter “3. Operation of Each Part.”
If the microscope has not yet been assembled, see chapter “4. Assembly” first.
Turn on the power.
(Flip the switch to the
« | ” side.)
As soon as the power
comes on, the switch
lights.
Flip the switch to
the “ | "side.
Set the brightness adjuster
to give the desired
brightness.
Pressing the auto-photo
switch will set the voltage
for the brightness that
provides the best color
reproduction. (p. 21)
Adjust the
Insert filters ND 16 or 4 Insert filters. — brightness.
and NCBII into the Auto-photo switch
optical path. can be pressed.
ND 16 or 4 reduces glare in
the binocular eyepiece.
NCB11 improves color
reproduction. (p. 20)
Move the 10x objective
into the optical path.
Rotate the revolving
nosepiece until the 10x
objective clicks into place.
El Set the
10x objective.
Set the specimen in place
with the cover glass facing
up.

2. Microscopic Procedure
Raise the condenser as
high as it will go.
Fully open the field
diaphragm and condenser
| aperture diaphragm.
Move the specimen until
the
portion to be viewed is
in the optical path. (p. 20)
Loosen the coarse focus
stopper ring.
Rotate it in the direction
opposite the arrow on the
base as far as it will go.
(p. 14)
I Raise the —
condenser.
Fully open the condenser Fully open the
aperture diaphragm. field diaphragm.
El Set the portion
El Loosen the coarse to be viewed in
focus stopper. the optical path.
+

Set the optical path to
100% of the binocular
|| eyepiece when using a
| triocular eyepiece tube.
2 (p.15)
Focus on the specimen.
(p. 13)
Adjust the diopter rings of
both eyepieces. (p. 16)
Adjust the interpupillary
distance. (p. 16)
Focus on
the specimen.
o ins
li
Set the optical path
to 100% of the
binocular eyepiece.
Adjust the
diopter rings.
Adjust the
interpupillary
distance.
10

2. Microscopic Procedure
Focus and center the
condenser. (p. 17)
Switch to any desired
objective and view the
| specimen.
Rotate the revolving
nosepiece to its click-stop
© position.
Readjust the focus.
Use ND filters to adjust
the brightness.
[i
Focus
the
LU
|
condenser.
Center the
e600 condenser.
{7A Adjust the
brightness with
回 Swi
回
Switch
the
ND
filters.
objective.
A
IB Readjust the =
focus.
11

2. Microscopic Procedure
Close the field diaphragm
so that it is just outside the
lose th denser
field
of
view.
(p.
19)
EC
e
conden
aperture diaphragm.
Close the condenser
aperture diaphragm to
¿about 70 — 80% of the
numerical aperture of the
objective. (p. 18)
I] Close the field diaphragm.
Cura portion of the field of view is dark, adjust the condenser centering screws. If that does not resolve
the problem, check the following items:
* Filter insertion/removal (p. 20)
¢ Optical path selection for the eyepiece tube (p. 15)
* Position of the revolving nosepiece (p. 11)
* Revolving nosepiece installation (p. 28)
+ Lamp installation (p. 26)
* Condenser focusing and centering (p. 17)
+ Field and aperture diaphragms (p. 18 and 19)
¢ Condenser installation (p. 27)
M If it is not possible to focus on the specimen, check the following items:
* Mounting of the specimen (p. 31 and 32)
* Release of the coarse focus stopper (p. 14)
¢ Thickness of the cover glass (0.17 mm is standard) (p. 31 and 32)
12

Operation of Each Part
1 Coarse and fine focus knobs
| 1. Rotation of the coarse and fine focus knobs and
vertical movement of the stage NS
Rotating the fine focus knob one step moves the stage 1 um.
Rotating the fine focus knob one complete turn moves the stage 0.1 mm.
Rotating the coarse focus knob one complete turn moves the stage 12 mm.
The coarse/fine focus stroke (range of vertical motion) for the stage is 2 mm up and 23 mm down
from the reference (focused) position.
Never attempt either of the following actions
These actions will damage the microscope.
¢ Rotating the left and right knobs in opposite
directions at the same time.
« Continuing to rotate the coarse focus knob
after the stage has reached the limit of its mo-
tion.
| 2. Adjusting the torque of the coarse focus knob BE
It is possible to adjust the torque of the coarse focus
knob.
\ \ \ \ \
To increase the torque, turn the torque adjustment ring
(TORQUE) located behind the coarse focus knob in the \ \ \ \ \
direction of the arrow on the microscope base (i.e., the \ \ \ \
counter-clockwise direction). To reduce the torque, turn
\ い
VA
the ring in the direction opposite to the arrow (i.e., the \
clockwise direction).
To increase
the torque
13

3. Coarse focus stopper NS
Clamp the coarse
focus stopper
Purpose of the coarse focus stopper
The coarse focus stopper marks the stage position at which the specimen is in focus by restrict-
ing the movement of the coarse focus knob. (Movement of the stage by the fine focus knob is
not restricted.)
Once the coarse focus stopper has been clamped in position, the coarse focus knob cannot be
used to move the stage any higher. In effect, once the coarse focus knob has been clamped in
place at the focus position, a rough focus can be attained the next time simply by turning the
coarse focus knob as far as it will go. This feature is convenient when viewing similar speci-
mens one after another.
Using the coarse focus stopper
With the specimen in focus, turn the coarse focus stopper ring as far as it will go in the direction
of the arrow on the base of the microscope (about 3/4 revolution). The coarse focus stopper is
now clamped in position.
When changing the specimen, lower the stage by turning only the coarse focus knob.
After changing the specimen, gently raise the stage by turning only the coarse focus knob as far
as it will go.
The specimen should be roughly in focus when the stage has been raised as far as it will go; use
the fine focus knob to bring the specimen into perfect focus.
If the coarse focus stopper is not being used, be sure to turn the coarse focus stopper ring in the
direction opposite to the arrow on the microscope base as far as it will go.
14

3. Operation of Each Part
2 Eyepiece tube optical path selection
= (T eyepiece tube, F eyepiece tube)
The optical path selection lever can be used to select the way to divide the amount of light between
the binocular part and the vertical tube.
Light proportion Light proportion
Lever binocular vertical Lever binocular vertical
position part tube position part tube
Ш
100 : ο
FAT]
sino—-
1]
100
:
0
/
„a
0
:
100
BINO&
F- PHOTOT [2] 20 : 80 o em 00
FEI PHOTO- 0 : 100 BINO PHOTO
Tİ
13]
+ PS
T eyepiece tube F eyepiece tube
Vi. vertical tube adapter
A photomicrographic vertical tube adapter is pro-
vided as standard equipment that allows a photomi-
crographic equipment to be installed. To install the
adapter, insert it into the vertical tube and clamp
three screws with the provided screwdriver. Re-
place this adapter with the optional TV vertical tube
adapter when using a TV camera.
2. T and F eyepiece tubes lever clicking
There is a switch identified by “NO CLICK” on the
bottom surface of the eyepiece tube. Turn the switch
in the direction of the arrow with the hexagonal
screwdriver provided to disengage the clicking ac-
tion of the optical path selection lever. Disengag-
ing the clicking action minimizes small vibrations
produced by operating the lever.
15

3 Diopter adjustment
xd Engraved line
Diopter adjustment "0" position '
=
Diopter adjustment compensates for differences in eyesight between the left and right eyes. In
addition to making observation through both eyes easier, this adjustment also reduces the difference
in focus when changing the objectives. Adjust the diopter setting for both eyepieces.
1 Turn each diopter adjustment ring on both eyepieces to align the edge of the ring with the
engraved line. This is the “O” position.
2 Perform steps MJ to IJ in chapter “2. Microscopic Procedure” so that the specimen is in focus
with the 10x objective.
3 Move the 40x objective into the optical path. Turn the coarse and fine focus knobs to focus
on the specimen.
4 Move the 4x or 10x objective into the optical path.
5 Turn the diopter adjustment ring on each eyepiece, not the coarse and fine focus knobs,
to bring the specimen into focus, while peering through the right eyepiece with your right
eye and the left eyepiece with your left eye.
6 Repeat steps 3 to 5 twice.
Interpupillary distance adjustment
Before adjusting the interpupillary distance, performs steps MN to KE in chapter “2. Microscopic
Procedure” so that the specimen is in focus with the 10x objective.
Adjust the interpupillary distance so that the view field for each eye is at the same position on the
specimen.
Doing so will make observation through the binocular eyepiece with both eyes easier.
16

3. Operation of Each Part
っ Condenser
N
一
1. Focusing and centering
e
Eyepiece view field yepiece view field
Focus and center the condenser so that the light passing through the condenser forms the image at
the correct position on the specimen (i.e., at the center of the optical path).
Before focusing and centering the condenser, perform steps I to KE] in chapter “2. Microscopic
Procedure” so that the specimen is in focus with the 10x objective.
Close the field diaphragm to its minimum setting.
Turn the condenser focus knob so that the image of the field diaphragm is formed on the
specimen.
Make rough adjustments with the condenser centering screws so that the image of the field
diaphragm appears at the center of the eyepiece view field.
Move the 40x objective into the optical path. Turn the fine focus knob to focus on the
specimen.
Turn the condenser focus knob so that the image of the field diaphragm is formed on the
specimen.
Adjust the condenser centering screws so that the image of the field diaphragm appears at the
center of the eyepiece view field. This adjustment is easier to make if you adjust the size of
the field diaphragm to be slightly smaller than the eyepiece view field.
17

§ 2. Condenser aperture diaphragm
Numerical
Plan 40X _ Aperture
40x
/:0.75°
RE
©
-WD
22
0.75X0.7~0.8=0.525~0.6
The aperture diaphragm is important because it is related to the resolution, contrast, depth of focus
and brightness of the optical image. Turning the condenser aperture diaphragm ring changes the
size of the aperture diaphragm.
As the aperture diaphragm is stopped down, resolution and brightness are reduced while contrast
and depth of focus are increased. Conversely, as the aperture diaphragm is opened, resolution and
brightness are increased while contrast and depth of focus are reduced. It is not possible to adjust
one pair of characteristics without affecting the other. Generally, a satisfactory image with appro-
priate contrast can be obtained with an aperture setting that is 70% to 80% of the numerical aperture
of the objective. The numerical aperture is indicated on the barrel of each objective.
An indication of 40x/0.75 means that the magnification is 40x and the numerical aperture is
0.75.
If the aperture diaphragm is stopped down too far, the resolution is reduced; therefore, except when
viewing a nearly transparent specimen, we do not recommend stopping down the aperture to less
than 60% of the numerical aperture of the objective.
Adjusting the size of the aperture diaphragm according to the condenser scale
Since the condenser scale indicates the numerical aperture, adjust the aperture diaphragm ring
according to the scale. (Normally, the index on the aperture diaphragm ring should be aligned
with the scale line corresponding to 70% to 80% of the numerical aperture of the objective.)
Adjusting the size of the aperture diaphragm by using a centering telescope
(sold separately)
Remove one of the eyepieces and, by using a special adapter (sold separately), mount a centering
telescope in its place. Turn the aperture diaphragm ring to stop down the aperture diaphragm as
far as possible. While holding down the flange of the telescope, turn the eyepiece of the center-
ing telescope and focus on the aperture diaphragm.
Turn the aperture diaphragm ring to adjust the size of the aperture diaphragm. (Normally, the
aperture diaphragm should be adjusted so that it is about 70% to 80% of the size of the field
being viewed.)
Remove the centering telescope and adapter, and then re-fit the eyepiece.
18

3. Operation of Each Part
3. Objective and condenser combinations
|
C -
ondenser та Swing-out Achromat Abbe 00 .
Objective aplana condenser condenser condenser magnification
magnification condenser condenser
1x x x x x
2x x x x © (Note 2)
© (Note 3)
4x x O (Note 1) | O (Note 1)
10x to 100x © O O O x
© :Optimum ©: Appropriate — X: Inappropriate
Note 1: The entire view field may not be satisfactory when a UW eyepiece is used.
Note 2: Indoor lighting and similar light sources may be reflected from the surface of the condenser
lens and be visible within the view field. When this happens, either dim the indoor lighting
or prevent strong outside light from entering the stage.
Note 3: Swing out the top lens before usage.
¢ Depending on the type of objective, the whole numerical aperture of the objective may not be
covered. For example, when an objective with an N.A. of 1.4 is used, the aperture size of the
swing-out condenser or the abbe condenser will only be about 65% of the objective's N.A. even
when it is fully opened.
* Refer to the condenser instruction manual for details of universal and phase contrast condensers.
6 Field diaphragm
The field diaphragm restricts illumination to the area on the specimen being viewed. Turning the
field diaphragm ring changes the size of the field diaphragm. For normal observation, the size of the
diaphragm should be slightly larger than the boundary of the view field. If a broader area than
necessary is illuminated, stray light will enter the view field, creating flaring and reducing the con-
trast of the optical image. The correct setting of the field diaphragm is especially important in
photomicrography; generally, good results are obtained by stopping down the field diaphragm to
just slightly larger than the area that will be reproduced on the film, i.e., the size of the photo frame.
19