Nikon DIAPHOT 300 User manual

Ill
Instructions
NIKON CORPORATION

Thank you for your purchase of Nikon’s Inverted
Microscope Diaphot 300. Please read this instruction
manual thoroughly in order to become acquainted with the
complete system and its operation. We hope your Diaphot
300 will be of lasting service.
Nikon reserves the right to make such altera¬
tions in design as may be considered necessary
in the light of experience. For this reason,
particulars and illustrations in this handbook may
not conform in every detail to models in current
production.

Handling Precautions
1. Handle Carefully!
Handle the microscope gently, taking care to avoid sharp impacts.
2. Purpose
Use the microscope only for microscopic observation. Do not use it for
any other applications.
3. Microscope Location
Select a location with limited exposure to dust, vibration, high temperature
and humidity (40°C, 80% or more), and direct sunlight. Leave a certain
space between the microscope and the nearby wall to allow the user to
look at the warning labels on the lamphouse.
4. 12V100W Power Supply Unit
The power supply unit specified on page 53 "ELECTRICAL
SPECIFICATIONS" is the power supply unit to light up the Nikon
microscopes' 1 2V100W lamp. Do not use it for other purposes.
Also, the user may not use the power supply unit if it is broken, and may
not open the cover of the power supply unit.
5. Line Voltage
Confirm that the input voltage to the 1 2V1 00W power supply unit
corresponds to your line voltage. Be sure to use the specified power
supply unit (described in page 53 "ELECTRICAL SPECIFICATIONS").
6. Light Source
The microscope uses a 1 2V, 1OOW halogen lamp bulb as the standard
light source. Do not use any lamp not described in the "ELECTRICAL
SPECIFICATIONS" (on page 53). If a lamp bulb of over-rated wattage is
used, the light adjusting circuit may be damaged.
7. With Lamp On —CAUTION —
Do not touch the lamphouse or place any heat-sensitive object near it,
since the lamphouse becomes extremely hot during use. Also use great
care not to bring volatile substances (such as gasoline, thinner, alcohol,
etc.) close to the lamphouse. Such exposure may result in inflammation
or other dangers.
8. Replacing the Halogen Lamp
Before replacing the 12V, 100W halogen lamp, turn off the power switch
and unplug the power cord. When replacing the halogen lamp, wait until
it cools down. Do not touch the glass of the halogen lamp with bare
hands.

9. Dirt on Lens
Do not leave dust, dirt or fingerprints on the lens or lamp. Dirt or stains
on the lens or mirror will deteriorate image quality.
10. Focusing Knobs
Never attempt to turn the right-hand and left-hand focusing knob in
opposite directions at the same time as it will result in defects or damages.
An attempt to turn the coarse focusing knob further after it reaches its limit
will cause defects or damages. Never turn it forcedly.

Care and Maintenance
1. Lens Cleaning
Dust is best removed with a soft brush or gauze. More persistent dirt,
such as fingerprints, grease and oil, may be removed with soft cotton,
lens tissue, or gauze lightly moistened with absolute alcohol (methyl
alcohol or ethyl alcohol).
Use only xylene to clean immersion oil off objective surfaces.
Do not use xylene to clean the entrance lens at the bottom of the eyepiece
tube or prism surface of the eyepiece tube.
Absolute alcohol and xylene are quite inflammable. Use great care when
handling them and when setting the power switch on and off. Be very
careful with fire.
2. Cleaning Painted Surfaces
Avoid use of any organic solvents (such as alcohol, ether, thinner, etc.) to
clean the painted or plastic surfaces of the instrument. We recommend
that these parts be cleaned with silicon cloth.
3. Never Dismantle
Never attempt to dismantle the instrument, thereby avoiding the possibility
of impaired operational efficiency and accuracy.
4. When Not In Use
When the microscope is not in use, cover it with the vinyl cover, and store
it in a dry place not subject to mold.
We especially recommend that the objectives and eyepieces be kept in a
container (such as a desiccator) with desiccant in it.
5. Periodical Inspection
To maintain the performance of the microscope, we recommend you to
check the microscope periodically. (For details of inspection, contact your
dealer or nearest Nikon representative.)

I. NOMENCLATURE
CONTENTS
Handling Precautions
Care and Maintenance
I . NOMENCLATURE. 1
II. BASIC MICROSCOPY. 4
III. PHOTOMICROGRAPHIC PROCEDURE. 1 2
IV. OPERATIONS ON COMPONENTS . 16
1 . ON/OFF switching of power. 1 6
2. Adjusting Brightness.17
3. Changing Optical Paths.19
4. Filters.20
5. Field Aperture Diaphragm . 21
6. Condenser Aperture Diaphragm . 22
7. System Condenser Turret Unit . 24
8. Objectives.26
9. Turret Assembly.28
10. Diopter Adjustment.29
1 1 . Adjusting Interpupillary Distance .30
1 2. Photomask.31
1 3. Eyepiece tube.32
14. Focusing Device.33
15. Refocusing Stopper . 34
16. Handling Condenser Flolder . 35
17. Tiltable Illumination Pillar.36
18. Rectangular Mechanical Stage.38
19. Photomicrography.39
V. ASSEMBLY . 4 2
VI. SYSTEM DIAGRAM . 4 8
VII. TROUBLESHOOTING . 4 9
• ELECTRICAL SPECIFICATIONS . 5 3
II. BASIC MICROSCOPY
III. PHOTOMICROGRAPHIC
PROCEDURE
IV. OPERATIONS ON
COMPONENTS
V ACCPMRIV
V , MOO Cl VIDL I
VI. SYSTEM DIAGRAM
YU. TROUBLESHOOTING
• ELECTRICAL
SPECIFICATIONS

I. NOMENCLATURE
1. System Components (Right-hand)
Supplementary lens pocket
Field aperture diaphragm lever
Figure 1-1

2. System Components (Left-hand)
Condenser
refocusing stopper
Condenser mount rotation clamp screw
Condenser holder
Module
Condenser turret
Annular diaphragm
centering screw
Condenser lens
Objective
Revolving
nosepiece
Side port
Coaxial coarse/fine
focusing knob
Refocusing stopper
Light intensity control dial
Condenser mount
Condenser centering
screw
System condenser
turret unit
Bertrand lens
focusing screw
Eyepiece tube port
Front port
Figure 1 -2
2

3. System Components (Rear)
Lamphouse cable connector
Figure 1-3
3

II. BASIC MICROSCOPY
For the normal microscopy, carry out steps 1 . to 14. For phase contrast
microscopy, carry out steps 1 . to 18. For detailed operations, see Section
IV. "OPERATIONS ON COMPONENTS ", from page 16.
If the microscope has not been assembled, first read Section V . "ASSEMBLY" from
page 42.
1. T urn on the power.
After confirming that the power supply
is set for the correct line voltage, turn
on the power switch. Set the light
intensity control dial on the power
supply unit to CTRL.
Figure 2-1
2. S et the lamp voltage to 6.
Rotate the light intensity control dial on
the left side of the base to the "6"
position. (If the light intensity is too
high or too low, adjust it properly.)
3. S et the optical path for the observation.
Set the turret assembly to 0. (Eyepiece
tube BT1 only.)
Set the optical path changeover dial on
the right side of the base to A.
Figure 2-3