Nikon ECLIPSE Ni-E User manual

Bright/Dark-field
Microscopy
Before Microscopy
Operation Flowchart
Components
Safety Precautions
Notes on Handling
the Product
Individual
Operations
Phase Contrast
Microscopy
Differential
Interference Contrast
Microscop
y
Epi-fluorescence
Microscopy
Upright Motorized Microscope
Instruction Manual
Operation
M571 E 11.8.NF.1 (1/2)
*M571EN01*

Introduction
i
Introduction
Thank you for purchasing a Nikon product.
This instruction manual is written for users of the Nikon ECLIPSE Ni-E microscope. To ensure correct usage, read this
manual carefully before operating this product.
●No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon.
●The contents of this manual are subject to change without notice.
●The equipment described in this manual may differ from the actual product in its appearance.
●Although every effort has been made to ensure the accuracy of this manual, errors or inconsistencies may remain. If
you note any points that are unclear or incorrect, please contact your nearest Nikon representative.
●Some of the equipment described in this manual may not be included in the set you have purchased.
●If you intend to use any other equipment with this product, read the manual for that equipment too.
●If this equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may
be impaired.
●Training: This product can be used without special training, provided that this manual is read thoroughly before use. Kindly
contact your nearest Nikon representative if you have any questions, find any errors, or wish to provide us with your
opinion.

Introduction
ii
Contents of the Manual
The instruction manual for ECLIPSE Ni-E is provided in two volumes.
Operation (This manual)
Safety Precautions
Components
Microscopy Operations
Before Microscopy
Operation Flowchart
Bright/Dark-field Microscopy Procedure
Epi-fluorescence Microscopy Procedure
Differential Interference Contrast Microscopy
Procedure
Phase Contrast Microscopy Procedure
Individual Operations
Assembly/Maintenance
Assembly
Troubleshooting
Maintenance and Storage
Specifications
Before reading the “Assembly/Maintenance” manual,
read the “Safety Precautions” in the “Operation”
manual.
Symbols Used in This Manual
The following symbols are used in this manual.
Symbols for Safety
WARNING
CAUTION
Highlights important information that should be noted for safety. Read “Safety Precautions” for details.
Other Symbols
Indicates information you should note or comply with to prevent defects or malfunction of this product.
Indicates information you should be aware of in using this product, as well as other useful information.

Summary of Contents
iii
Bright/Dark-field
Microscopy
Before Microscopy
Operation Flowchart
Components
Safety Precautions
Notes on Handling
the Product
Individual
Operations
Phase Contrast
Microscopy
Differential
Interference Contrast
Microscop
y
Epi-fluorescence
Microscopy
Summary of Contents (See the next page for the detailed contents.)
Introduction
Contents of the Manual
Symbols Used in This Manual
Safety Precautions
Notes on Handling the Product
Components
(System Configuration and Controls for Each Microscopy)
Before Microscopy
(Information Setting for Motorized Operation)
Operation Flowchart
Bright/Dark-field Microscopy
Epi-fluorescence Microscopy
(Epi-fluorescence, Epi-fluorescence/Differential Interference
Contrast, Epi-fluorescence/Phase Contrast)
Differential Interference Contrast
Microscopy
Phase Contrast Microscopy
Individual Operations
(Display Panel, Operation Buttons, Instructions for
Each Part/Device, Image Capture)

Contents
iv
Contents
Introduction .........................................................................................................................i
Contents of the Manual ....................................................................................................ii
Symbols Used in This Manual ..........................................................................................ii
Summary of Contents ....................................................................................................... iii
Safety Precautions............................................................................................................ vi
WARNING and CAUTION Symbols ................................................................................vi
Meaning of Symbols Used on the Product......................................................................vi
WARNING ...............................................................................................................vii
CAUTION .................................................................................................................ix
Notes on Handling the Product......................................................................................... xi
Components............................................................................................. 1
1 System Configuration and Controls for Bright/Dark-field Microscopy .......................2
1.1 Components ....................................................................................................... 2
1.2 Controls .............................................................................................................. 3
1.3 Buttons ............................................................................................................... 4
1.4 Connectors ......................................................................................................... 5
2 System Configuration and Controls for Epi-fluorescence Microscopy ......................6
3 System Configuration and Controls for Differential Interference Contrast
Microscopy ................................................................................................................8
4 System Configuration and Controls for Phase Contrast Microscopy ........................9
Microscopy Operations......................................................................... 11
1 Before Microscopy ..................................................................................................12
2 Operation Flowchart................................................................................................14
3 Bright/Dark-field Microscopy Procedure..................................................................18
4 Epi-fluorescence Microscopy Procedure.................................................................33
5 Differential Interference Contrast Microscopy Procedure........................................49
6 Phase Contrast Microscopy Procedure...................................................................67
Individual Operations ............................................................................ 83
1 Display Panel Details ..............................................................................................84
2 Using Operation Buttons on Ni-E ............................................................................87
2.1 Front Operation Button..................................................................................... 87
2.2 Right Operation Button..................................................................................... 88
2.3 Left Operation Button ....................................................................................... 89
3 Adjusting the Brightness of a Diascopic Image.......................................................90
3.1 Adjustment by Lamp Voltage ........................................................................... 90
3.2 Adjustment with ND Filters............................................................................... 91
4 Focusing on the Sample (Vertical Stage Movement) ..............................................92
4.1 Proper Focusing Procedure ............................................................................. 92
4.2 Focus Knobs on the Main Body ....................................................................... 93
4.3 Resetting the Z-axis Coordinate....................................................................... 94
4.4 Refocusing ....................................................................................................... 95
5 Bringing the Target into the Optical Path (Horizontal Stage Movement, Rotation) ..96
5.1 Operating the Rotatable Ceramic Coated Stage.............................................. 96

Contents
v
5.2 Operating the Motorized XY Stage................................................................... 98
6 Adjusting the Diopter...............................................................................................99
7 Focusing and Centering the Condenser ...............................................................100
8 Adjusting the Aperture Diaphragm ........................................................................101
9 Using the Condenser ............................................................................................103
9.1 Compatibility of Condensers with the Magnification of Objectives ................ 103
9.2 Using the NI-CUD-E Motorized Universal Condenser (Dry) .......................... 104
9.3 Using the NI-CUD Universal Condenser (Dry)............................................... 105
9.4 Using the D-CUO DIC Condenser (Oil) ......................................................... 107
10 Adjusting the Field Diaphragm ..............................................................................108
11 Switching the Objective.........................................................................................109
11.1 Motorized Nosepiece Operation..................................................................... 109
11.2 Intelligent/Manual Nosepiece Operation ......................................................... 111
12 Switching the Optical Path of the Tube ................................................................. 112
12.1 Optical Path Switching of the Motorized Quadrocular Tilting Tube.................112
12.2 Switching the Optical Path in a Manually-Operated Tube ..............................113
13 Adjusting the Binocular Section ............................................................................114
13.1 Adjusting with the Binocular Section of the Tube............................................114
13.2 Using the Eyelevel Riser .................................................................................114
14 Oil/Water Immersion.............................................................................................. 115
15 Dark-field Microscopy............................................................................................ 117
16 Epi-fluorescence Microscopy ................................................................................ 118
16.1 Epi-fluorescence Cube Turret Operation (Switching Excitation Methods)......118
16.2 Selecting Filters.............................................................................................. 120
16.3 Protecting the Sample and Preventing It from Decoloration
(Using the Shutter) ......................................................................................... 122
16.4 Adjusting the Brightness of the Fluorescent Image (Using ND Filters
and the Aperture Diaphragm)......................................................................... 124
16.5 Restricting the Illumination to the Area of the Specimen being Viewed
(Centering and Adjusting the Field Diaphragm)............................................. 126
16.6 Changing the Waveform Characteristics of the Excitation Light (D-FB
Excitation Balancer) ........................................................................................ 127
16.7 Switching the Excitation Filter (Motorized Excitation Filter Wheel)................ 128
16.8 Switching the Barrier Filter (Motorized Barrier Filter Wheel) ......................... 129
16.9 Other Notes on Epi-fluorescence Microscopy................................................ 129
17 Differential Interference Contrast Microscopy .......................................................130
17.1 Tips for Differential Interference Contrast Microscopy................................... 130
17.2 Using Optical Elements.................................................................................. 131
18 Phase Contrast Microscopy ..................................................................................132
18.1 Tips for Phase Contrast Microscopy .............................................................. 132
18.2 Using Optical Elements.................................................................................. 133
19 Useful Functions ...................................................................................................134
19.1 MODE............................................................................................................. 134
19.2 Interlocking Function ...................................................................................... 138
20 Using the Ergo Controller......................................................................................141
21 Using the Joystick Controller.................................................................................144
22 Capturing Images..................................................................................................145
23 Operation on DS-L3 ..............................................................................................150
23.1 Setting Up the Microscope ............................................................................. 150
23.2 Controlling the Microscope............................................................................. 166

Safety Precautions
vi
Safety Precautions
Notes on Handling
the Product
Safety Precautions
To ensure correct and safe operation, read this manual before using this product.
WARNING and CAUTION Symbols
Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow
the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual
carefully before using this product. Do not discard this manual and keep it handy for easy reference.
Safety instructions in this manual are marked with the following symbols to indicate their importance. For your safety,
always follow the instructions marked with these symbols.
Symbol Description
WARNING Disregarding instructions marked with this symbol may lead to serious injury or death.
CAUTION Disregarding instructions marked with this symbol may lead to injury or property damage.
Meaning of Symbols Used on the Product
When appearing on this product, the symbols below indicate the need for caution at all times during use. Read the
relevant instructions in this manual before attempting to use or adjust any part to which the symbol has been affixed.
Biohazard
This symbol is affixed to the front of the stand of this product, to call your attention to the following:
●WARNING: Using this product may constitute a biohazard risk if a sample comes into contact with this
product.
●To avoid biohazard contamination, do not touch the contaminated portion with bare hands.
●Decontaminate the contaminated part according to the standard procedure specified for your
laboratory.
Precautions against heat
This symbol is affixed to the lamphouse to call your attention to the following:
●During and immediately after a period of illumination, the lamp and surrounding areas (including the
lamphouse) are very hot.
●Risk of burns. Do not touch the lamp or surrounding areas during or immediately after a period of
illumination.
●Make sure the lamp and surrounding areas have cooled sufficiently (which may take approx. 30
minutes) before attempting to replace the lamp.
Precautions against heat
This symbol is affixed to the top of the arm (both standard and contact), but “Precautions against heat” is
not required when using in combination with Ni-E Microscope.

Safety Precautions
vii
Safety Precautions
Notes on Handling
the Product
WARNING
1 Do not disassemble.
Disassembling this product may result in electric
shock or malfunction. Malfunction and damage
due to disassembling or modification are
unwarranted.
Do not disassemble parts other than those
described in this manual. If you experience
problems with this product, contact your nearest
Nikon representative.
2 Read the instruction manuals carefully.
To ensure safety, thoroughly read this manual and
the manuals for other equipment to be used with
this product. Particularly, all warnings and
cautions given at the beginning of each manual
must be observed.
Safety is a top design priority for Nikon products.
Safety is ensured as long as the user observes all
of the warnings and cautions given in the
manuals, and uses the system only for its
intended purpose. However, failure to heed the
warnings and cautions given in the manuals,
subjecting the system to shock or impact, or
attempting to disassemble the system may result
in unexpected accidents and injury.
Product with NI-FLEI epi-fluorescence
attachment:
The light source used for Epi-fluorescence
microscopy (HG Precentered Fiber Illuminator)
requires special care during handling because of
its characteristics. Be sure to refer to the manual
for the light source being used.
3 Notes on the power cord
Be sure to use the specified power cord. Use of
other power cords may result in malfunction or
fire. This product is classified as having Class I
protection against electric shock. Make sure this
product is connected to an appropriate protective
earth terminal.
See Chapter 4 “2. Performance Properties” in the
“Assembly/Maintenance” instruction manual for
details on the designated power cords.
●To prevent electric shock, always turn off the
power switch (press to the “O” position) for the
microscope before connecting or disconnecting
the power cord.
4 Heat from the illuminator
During and immediately after a period of
illumination, the lamp and surrounding areas
(including the lamphouse) are very hot.
●Do not touch the lamp or surrounding areas
during or immediately after a period of
illumination. There is a risk of burn if you touch
the hot area.
●Always attach the lamphouse cover when using
this product.
●Make sure the lamp and surrounding areas
have cooled sufficiently (which may take
approx. 30 minutes) before attempting to
replace the lamp.
●To avoid the risk of fire, do not place fabric,
paper or highly flammable volatile materials
such as gasoline, petroleum benzine, paint
thinner, or alcohol near the lamphouse while
the lamp is lit or for a period of approximately
thirty minutes after the lamp is turned off.

Safety Precautions
viii
Safety Precautions
Notes on Handling
the Product
WARNING
5 Hazards of mercury lamps
(when using the NI-FLEI Epi-fluorescence
attachment)
The light source used with the epi-fluorescence
attachment (HG Precentered Fiber Illuminator)
requires special care during handling because of
its characteristics. For safe and correct use of this
system, carefully read the warnings below. Keep
in mind all potential hazards. Additionally, carefully
read the manual for the illuminator and the manual
from the lamp manufacturer (if provided), then
follow the instructions given therein. Failure to
heed the warnings and cautions given in the
manuals, subjecting the system to shock or
impact, or attempting to disassemble the system
may result in unexpected accidents and injury.
●Ultraviolet light
When lit, mercury lamps radiate ultraviolet light
that can damage the eyes and skin. Direct
viewing of the light may result in blindness.
When changing filter cubes, always turn off the
light source of the Epi-fluorescence
attachment. Leaving the lamp turned on during
filter cube replacement may result in ultraviolet
exposure.
Leave the D/UV slider of the D-ES EPI ND
slider within the optical path. Removing the
D/UV slider from the optical path will cause
your eyes to become exposed to ultraviolet
light.
●High-pressure gas
The lamps contain sealed gas under very high
pressure. And the pressure increases when the
lamp is on. If the lamp is scratched, fouled,
subjected to high external pressure or physical
impact, or used beyond its service life, the
sealed gas may leak or the lamp may burst,
resulting in gas inhalation, injury from glass, or
other accidents.
●Heat
When the lamp is lit, the lamp and surroundings
will become extremely hot. Do not touch the
lamp with bare hands or place flammable
materials near the lamp. Failure to comply may
result in burns or fire.
●Designated lamp
Be sure to use the designated lamp. Using
other types of lamps may result in accidents,
including bursting of the lamp.
6 Hazardous sample handling
This product is intended primarily for microscopic
observations and image capture of cells and
tissue set on glass slides.
Check to determine whether a sample is
hazardous before handling. If the sample is
hazardous, handle it according to the standard
procedure specified for your laboratory. If the
sample is potentially infectious, wear rubber
gloves and avoid direct contact with the samples.
If such a sample is spilled onto this product, the
portion must be decontaminated in a safety
manner. Consult your safety supervisor or safety
standard of your facility.

Safety Precautions
ix
Safety Precautions
Notes on Handling
the Product
CAUTION
1 Power shutdown
To prevent electric shock and/or malfunction,
always turn off the power switch(es) for this product
and the peripheral devices (press to the "O"
position) and unplug the power cord from the wall
outlet before assembling this product, connecting
or disconnecting cables, replacing lamps, or
cleaning this microscope and the objective.
2 Lamp replacement precautions
●To avoid burns, wait at least 30 minutes after
the lamp is turned off to give it sufficient time to
cool. To avoid electric shock or malfunctions,
never attempt to replace the lamp without first
turning off the power switches for this product
and the peripheral devices (press to the “O”
position) and unplugging the power cord from
the wall outlet.
●After replacing the lamp, make sure to reattach
the lamphouse cover. Do not use the product
without the lamphouse cover attached.
●Do not break used lamps. It should be disposed
of as industrial waste, in accordance with local
regulations and rules.
3 Designated lamp
Using power supplied from control box A to the
main body, halogen lamps of up to 12V-100W can
be lit. Always use the specified lamp and
lamphouse. Using unspecified products may
cause malfunctions.
Specified lamp: PHILIPS 7724 or
OSRAM HLX64623
Designated lamphouse:
NI-LH Precentered Lamphouse
4 Movement of motorized device
This product can be equipped with motorized
devices such as a motorized nosepiece and a
motorized epi-fluorescence cube turret, which can
be controlled from remote controllers and PCs.
To avoid unexpected injuries, note the following in
operating motorized devices.
●Before operation, check the state of the entire
microscope system to ensure safety when
operating the motorized devices.
●Keep your hands and fingers away from the
nosepiece, objectives, stage, and components
and specimen on the stage to avoid injuries
during operation.
5 Avoid contact with water or chemical
solutions.
Never expose this product to water or chemical
solutions, and avoid using this product in
circumstances where there is risk of exposure to
water or chemical solutions. Exposure of electric
parts (such as the HG Precentered Fiber
Illuminator) to liquids may cause a short circuit,
resulting in malfunction or abnormal heating. If
water or a chemical solution is splashed onto this
product, immediately turn off the power switches
for this product and the peripheral devices (press
to the “O” position) and remove the power cord
from the receptacle. Then wipe off moisture with a
piece of dry cloth or something similar. If water or
a chemical solution enters this product, stop using
the product, and contact your nearest Nikon
representative.
6 Remove any covers from the product before
switching on.
Do not use this product while it is covered with a
piece of cloth, etc. This will result in an abnormal
heating or a fire hazard. Do not cover this product
with a piece of cloth or similar while in use. The
system temperature will rise, resulting in a
malfunction.
7 Notes on Laying Cables
During a period of illumination, the lamphouse is
very hot. Try to lay cables so that they should not
contact the lamphouse.
8 Do not place any object on top of the
product.
Do not place any object on top of this product.
9 Cautions on assembling and installing the
product
●Take care to avoid pinching your fingers or
hands during product assembly and
installation.
●Scratches or fouling optical components (such
as lens and filters) with fingerprints, etc. will
degrade microscope images. Be careful to
avoid scratches or direct contact with the lens
and filters when assembling.
●The main body weighs approximately 11 kg.
This product is not designed to be portable.
When moving the microscope (i.e. to another
room within the facility), work in a team of at
least two persons, and firmly hold the base of
the product.
●Remove all attachments (if mounted) from the
microscope before moving the microscope.
●Do not place this product in a locker or cabinet.

Safety Precautions
x
Safety Precautions
Notes on Handling
the Product
CAUTION
10 Cautions on sustained observations
To relieve fatigue resulting from long observation
sessions, limit continuous observations to one
hour. Take at least 10 to 15 minutes breaks
between observation sessions. Adjust the layout
of other equipment used and the height of your
chair.
11 Cautions on use, transportation, and
storage
This product must be operated, transported, or
stored in accordance with the following conditions.
Installing this product in a hot, humid location may
result in the formation of mold or condensation on
lenses, impairing performance or causing
malfunctions.
●Operating conditions:
temperature: 0 to +40°C,
humidity: 60% RH max. (no condensation)
●Transporting/storage conditions:
temperature: -20 to +60°C,
humidity: 90% RH max. (no condensation)
See Chapter 3 “4 Transportation (Using the
fastening position mode switch)” in the
“Assembly/Maintenance” instruction manual for
details on fastening the system during its
transportation.
12 Cautions on the disposal of the product
To avoid biohazard risks, dispose of this product
as contaminated equipment in accordance with
the standard procedure specified for your facility.

Notes on Handling the Product
xi
Safety Precautions
Notes on Handling
the Product
Notes on Handling the Product
1 Handling the product carefully
This product is a precision instrument. Avoid
subjecting it to sudden impacts and shocks.
Even relatively minor impacts are capable of
affecting the precision of the objective.
2 Weak electromagnetic waves
This product emits weak electromagnetic waves. So
as to avoid degrading the performance of precision
electronic devices, do not install this product near
such devices. If TV or radio reception is affected,
move the TV or radio further from this product.
3 Scratches, dirt, and foreign particles on the lens
Scratches or fouling optical components (such as
lens and filters) with fingerprints, etc. will degrade
microscope images.
If these parts become dirty, clean them as described
in Chapter 3 “2.1 Cleaning Lenses” in the
“Assembly/Maintenance” instruction manual.
4 Installation location
This product is a precision instrument. Usage or
storage of this product in an inappropriate
environment may result in malfunction or a
degradation in precision. Consider the following
factors when selecting an installation location:
●Select a location free of vibration. Install this
product on a level surface.
●Install this product at least 10 cm away from
walls.
●Choose a location less exposed to hazards in the
event of collisions, earthquakes, or other
potential disasters. To keep this product from
falling, use a strong rope or other means if
necessary to secure it to the working desk or
other heavy, stable item. This product is provided
with a M6 screw hole on the right and left sides of
the recessed area at the lower back of the main
body.
●Select a layout that allows easy removal of the
power cord from the product's AC inlet in the
event of an emergency.
●Do not use a desk mat or similar.
●Avoid locations exposed to direct sunlight,
locations immediately under room lights, and
other bright locations.
●Light from room lights just above this product may
enter the objective as extraneous light. If possible,
switch off the room lights directly above this
product when making observations.
●Select a location with minimal dust.
●To avoid splashes, do not use this product near
water.
●Make sure the ambient temperature is 0 to
+ 40°C and humidity is 60% or less. When
transporting or storing this product, the ambient
temperature must be -20 to +60°C, with the
humidity at 90% RH max (with no condensation).
Installing this product in a hot, humid location
may result in the formation of mold or
condensation on lenses, impairing performance
or causing malfunctions.
●Do not place this product in a locker or cabinet.
5 Handing a Focus Knob
●Never turn the focus knobs on the right and left
sides of the microscope in opposite directions at
the same time. Doing so may damage this
product.
6 Protect the ports from dust and extraneous light
Always attach the supplied cap to any ports not in
use, a slider slot, and an objective mounting hole
when a tube, epi-fluorescence cube turret,
nosepiece or other devices are mounted. Otherwise,
extraneous light and dust may be trapped inside the
product.
7 Handling of filters (when using the epi-fluorescence
attachment)
●Excitation filters inside a filter cube are exposed
to strong light and degrade over time. Replace
them after the appropriate number of hours of
use.
●Filter characteristics may alter if the filter is
exposed to high humidity. To prevent changes or
degradation of filter characteristics, avoid using
or storing the filters under conditions of high
humidity or high temperature. Avoid subjecting
filters to rapid temperature changes. When a
filter is not in use, store in a desiccator or
hermetically sealed container with a drying
agent.
●Especially the filters in the nine types of filter
cubes listed below offer sharp, high-resolution
waveform characteristics superior to normal
filters. However, due to their sophisticated
coatings, they must be handled with special care.
Take care to avoid abrasion from cleaning.
Follow the description in Chapter 3, Section 2.1
“Cleaning Lenses” in the
“Assembly/Maintenance” instruction manual.
Single-band filter cubes: DAPI, FITC, TxRed,
GFP
Multi-band filter cubes: F-R, F-T, D-F, D-F-R,
D-F-T

Notes on Handling the Product
xii
Safety Precautions
Notes on Handling
the Product
8 Motorized devices
When using motorized devices, do not force the
motorized devices to move/stop by hand.
9 Vibration during motorized operation
While the product is designed to minimize the
amount of vibration during motorized operation,
note that there still may be some effect on the
microscopy results.
10 Unpacking and unlocking
1) Check the package contents.
●ECLIPSE Ni-E Main Body X1
●Hex driver X3
Hex wrench X2
●Toolbox X1
●Accessory sticker X2 types
(A blinder for the mounting hole for accessory
not in use on the top of the arm and
nameplate for an additional ND filter to the
ND filter cassette)
2) Before turning on the power, remove the
tightening screw from the front of the elevating
section. (See Chapter 1 “3 Assembly Method-2
Unfasten the elevating section.” in the
“Assembly/Maintenance” instruction manual for
details.)
3) Do not dispose of the packing materials. They
will be reused for transportation.

1
Components
1
Chapter
1
Components
This chapter shows figures of devices required for each motorized microscopy with name of components and controls.
The figures are examples of basic motorized systems. Manually-operated devices are also available.
For the list of components used for the ECLIPSE Ni-E microscope, see Chapter 1 “2 Components List” in the separately
provided “Assembly/Maintenance” instruction manual.

Chapter 1 Components
2
Components
1 System Configuration and Controls for Bright/Dark-field Microscopy
1.1 Components
IN
ND
8ND
32 NCB
11
OUT
50
60
70
•For dark-field microscopy, a dark-field module is required for the motorized universal condenser.
Eyepiece
Objective
Motorized quadrocula
r
tilting tube
(Model: NI-TT-E)
Motorized nosepiece
(Model: NI-ND6-E or NI-N7-E)
Substage (Model: NI-SSR)
NI-E right handle rotatable
ceramic coated stage
(Model: NIE-CSRR2)
Motorized universal condense
r
(Model: NI-CUD-E)
Ni-E main body
Precentered lamphouse (12V
100W) (Model: NI-LH)
Halogen lamp (100W)
(Model: PHILIPS 7724 or
OSRAM HLX64623)
Motorized DSC zooming port
for quadrocular tube (Model:
NI-RPZ-E
)
Camera head
Contact arm
(Model: NI-CAM)
Connector box
(Model: NIE-CB)
Motorized ND filter
wheel
(Model: NI-ND-E)
DS camera control unit
(Model: DS-L3)
(See the DS-L3 instruction manual.)
Control Box A
(Model: NI-CTLA)

Chapter 1 Components
3
Components
1.2 Controls
IN
ND
8ND
32 NCB
11
OUT
50
60
70
50
60
70
Condenser focus knob
Diopter
adjustment ring
Display panel
(See Chapter 3
“1 Display Panel Details”.)
Coarse focus knob
Condenser centering
scre
w
Right control buttons
(See “1.3 Buttons” on the
next page.)
Front control buttons
(See “1.3 Buttons” on the
next page.)
Left control buttons
(See "1.3 Buttons" on the
next page.)
Stage X knob
Coarse focus knob
Moving claw of the
specimen holde
r
Stage Y knob
Fine focus knob
Condenser focus knob
Condenser centering
screw
Stage rotation clamp
scre
w
Fine focus knob

Chapter 1 Components
4
Components
1.3 Buttons
Ni-E main body (Front) Control Buttons
ND
8
ND
32
OUT
IN
NCB
11
Ni-E main body (Right) Control Buttons
Ni-E main body (Left) Control Buttons
Changing function control button assignments
Buttons indicated with “(Factory setting: ...)” can be assigned different functions.
Different functions can be assigned from the DS-L3 camera control unit. See Chapter 3, Section 23 “Operation on DS-L3” -
23.1 “Setting Up the Microscope - (3) Configuring the Button Functions - (3-1) Ni-E microscope button”.
In the explanations of operations in this manual, functions of these buttons are assumed to be left as factory settings.
Z-RESET button
DISPLAY Next button
REAR optical path button
Sleep LED
Display panel
brightness adjustment
button
DISPLAY Previous button
BINO optical path button
Power LED
FRONT optical path button
Switch DISPLAY button
ND filter IN/OUT switch
FL CUBE CCW rotation button
(Factory setting: First epi-fluorescence
cube turret)
DIA field diaphragm
button
FL SHUTTER button
(Factory setting: Shutter built into
first epi-fluorescence cube turret)
CAPTURE button
(Factory setting: Camera
directly controlled b
y
DS-L3 connected to USB
connector)
DIA aperture diaphragm
button FL CUBE CW rotation button
(Factory setting: First epi-fluorescence
cube turret
)
Escape button
OBJ CW rotation button
(Factory setting: Nosepiece)
OBJ CCW rotation button
(Factory setting: Nosepiece)
Dia-illumination
ON/OFF switch
Dia-illumination brightness control knob
FUNCTION button
(Factory settings:
Button 1, 2: Condenser CCW, CW
rotation
Button 3, 4: Excitation filter wheel
CCW, CW rotation
Button 5, 6: Barrier filter wheel
CCW, CW rotation)

Chapter 1 Components
5
Components
ND
ERGO/JOY
DSC2
DIA SHUTTER STAGE
EX
1.4 Connectors
MODEL ECLIPSE Ni-E
NIKON CORPORATION
TOKYO, JAPAN
MADE IN JAPAN
This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions:
(1) this device may not cause harmful interference, and
(2) this device must accept any interference received, including interference that may cause undesired operation.
This Class A digital apparatus complies with Canadian ICES-003.
Cet appareil numérique de la classe A est confirme à la norme NMB-003 du Canada.
910001
CONTACT ARM2
DSC1
HGFIE
BOX
USB
EPI SHUTTER
1.
2.
3.
Do not touch the lamphouse while the lamp is lit.
The surface of the lamphouse becomes hot when the
lamp is on.
Turn off the power and allow the lamp and lamohouse
to cool enough before replacing the lamp.
Wait for at least 30 minutes after turning off the
lamp.
Use 12V100W HALOGEN lamp only.
HALOGEN 12V100W
952001
NI-LH
CAUTION!
-High Temperature-
JAPAN
Ni-E main body (Rear)
Ni - E CONTACT
ARM1
LAMP
FASTENING
POSITION SW
OFF ON
100-240V~ 5A 50/60Hz
MODEL NI-CTLA
MADE IN JAPAN
This device complies with Part
15 of the FCC Rules. Operation
is subject to the following
two conditions:
(1) this device may not cause
harmful interference, and
(2) this device must accept any
interference received,
including interference that
may cause undesired
operation.
This Class A digital apparatus
complies with Canadian ICES-003.
Cet appareil numérique de la
classe A est confirme à la
norme NMB-003 du Canada.
540001
Control Box A (Front/Rear)
CONTACT ARM1
(Connect to CONTACT ARM1 o
f
control box A)
EPI SHUTTER
(Connect to EPI motorized
shutter)
CONTACT ARM2
(Connect to CONTACT ARM2 o
f
Ni-E main body)
CONTACT ARM2
(Connect to CONTACT ARM2 o
f
contact arm)
USB
(Connect to DS-L3 or PC)
DSC1
(Connect to EXT.I/O of
DS-L3/U3)
HGFIE
(Connect to motorized HG
precentered fibe
r
ill
u
min
a
t
o
r
)
BOX
(Connect to Ni-E of control box A)
DSC2
(Connect to EXT.I/O o
f
DS-L3/U3)
ERGO/JOY
(Connect to ergo controlle
r
o
r
joystick controller)
ND
(Connect to the motorized
ND filter wheel)
EX
(Connect to motorized
excitation wheel)
STAGE
(Connect to motorized
XY stage controller)
DIA SHUTTER
(Connect to DIA
motorized shutter)
CONTACT ARM1
(Connect to CONTACT ARM1
of contact arm)
LAMP
(Connect to dia-illumination
lamphouse)
A
C inlet
(Connect to AC power using a
power cord)
Fastening position mode switch
(ON: fastening position mode,
OFF: normal mode)
Input voltage display label
Ni-E (Connect to BOX of Ni-E main body)
Power LEDPower switch

Chapter 1 Components
6
Components
2 System Configuration and Controls for Epi-fluorescence Microscopy
This section describes the components required for epi-fluorescence microscopy.
Fundamental components used in common with bright-field microscopy are omitted. For details, see “1 System
Configuration and Controls for Bright/Dark-field Microscopy.”
Components
IN
ND
8ND
32 NCB
11
OUT
1
2
A
λ
F. ST OP A. STOP
EX. ADJ
2
3
4
5
6
INTENSILIGHT
C
-
HGFIE
LAMP
POWER
RUN TIM E hr s.
50
60
70
•See the figure for bright-field system for control box A and DS-L3 DS Camera Control Unit .
Motorized epi-fluorescence
cube turret
(Model: NI-FLT6-E)
Epi-fluorescence attachment
(Model: NI-FLEI)
HG adapter (Model: C-HGFIB)
Light shielding
plate
Motorized HG precentered fiber illuminato
r
(Model: C-HGFIE)
(See instruction manual for HG precentered
fiber illuminator.)
HG fiber
(Model: C-HGFIF 15/30)