Nikon OPTIPHOT-POL User manual

Nikon
Polarizing Microscope
OPTIPHOT -POL
INSTRUCTIONS
NIPPON KOGAKU K. K.

CAUTIONS
oAvoid sharp knocks!
Handle the microscope gently, taking care
to avoid sharp knocks.
f}When carrying the microscope
When carrying the microscope, hold its arm
with one hand, supporting the bottom of
the microscope base with the other. The
instrument weighs about 10.5 kg. Do not
have the lamp housing carry any load.
8Place for using
Avoid the use of the microscope in a dusty
place, where it is subject to vibrations or
exposed to high temperatures, moisture or
direct sunlight.
oPower source voltage
In every case, make sure of the power
source voltage by means of the input
voltage change-over switch on the bottom
of the microscope base.
oLight source
Halogen lamp bulb to be used is 12V-
50W. Do not use 12V-l OOWhalogen lamp
bulb. If the lamp bulb of over-rated wat-
tage is used, light adjusting circuit will
damage.
Never connect the lamp housing cord to
the house current socket directly.
oIn lighting the lamp
Take care not to touch the lamp housing
being lighted, and don't bring inflammable
substances such as gasoline, thinner, and
alcohol near to the lamp housing, as some
parts of the lamp housing may take a high
temperature while the lamp is being light-
ed.
oExchanging the lamp bulb and fuse
Before replacing the lamp bulb or fuse,
turn 0 F F the power switch and disconnect
the plug of the power source cord.
In such cases as of replacement, do not
touch the lamp bulb with bare hands, im-
med iately after putting out the lamp.
«;) Dirt on the lens
Do not leave dust, dirt or finger marks on
the lens surfaces.
They will prevent you from clear observa-
tion of the specimen image.
oStrain-free glasses
The optical elements of this microscope
being constructed of strain-free glasses,
take particular caution in handling the
objectives and condenser lenses not to
cause strain to them.
4D> Focus knobs
Never attempt to adjust the tightness of
the right- and lefthand focus knobs by
turning the one, while holding the other in
th is model microscope, because of causi ng
disorder.

CARE AND MAINTENANCE CONTENTS
·············0
....... 0
·······8
NOMENCLATURE
II. ASSEMBLY.
I.
III. PREPARATION
1. Centering the Lamp 8
2. Interpupillary Distance Adjustment 0
3. Diopter Adjustment 0
4. Optical Path Change-over in the
Trinocular Eyepiece Tube "TP" 0
5. Centering the Objectives 0
6. Centering the Condenser Lens 4@
IV. MICROSCOPY 41
1. Operating Procedure 41
2. Manipulation of Each Element ~
1) Focusing ~
2) Condenser aperture diaphragm ~
3) Field diaphragm ~
4) Circular graduated stage ~
5) Objectives 4J)
6) Eyepieces 4J)
7) Achromat strain-free condenser 4J)
8) Bertrand lens 4D
9) 1/4 A& tint plate 4D
10) Dia-polarizer and analyzer 4D
11) Filters G)
12) Lowering the substage G)
13) Illumination system G)
V. PHOTOMiCROGRAPHy 4D
VI. ACCESSORIES ~
1. Senarmont Compensator ~
2. Quartz Wedge ~
3. Monocular Eyepiece Tube "AP" W
4. Universal epi-illuminator W
5. Attachable Mechanical Stage Type "E". ~
6. Universal Stage ~
VII. TROUBLE SHOOTING TABLE ~
REFERENCE ~
ELECTRIC SPECIFICATIONS 0
8Cleaning the painted surfaces
Avoid the use of any organic solvent (for
example, thinner, ether, alcohol, xylene
etc.) for cleaning the painted surfaces and
plastic parts of the instrument.
oCleaning the lenses
To clean the lens surfaces, remove dust
using a soft brush or gauze. Only for
removing finger marks or grease, should
soft cotton cloth, lens tissue or gauze
lightly moistened with absolute alcohol
(ethanol or methanol) be used.
For cleaning the objectives and immersion
oil use only xylene.
Observe sufficient caution in handling
alcohol and xylene.
8Never attempt to dismantle!
Never attempt to dismantle the instrument
so as to avoid the possibility of impairing
the operational efficiency and accuracy.
oWhen not in use
When not in use, cover the instrument with
the accessory vinyl cover, and store it in a
place free from moisture and fungus.
It is especially recommended that the
objectives and eyepieces be kept in an
airtight container containing desiccant.
oPeriodical checking
To maintain the performance of the instru-
ment, we recommend to check the instru-
ment periodically. (For details of this
check, contact our agency.)

I.NOMENCLATURE
Interpupillary distance scale
Diopter ring
CF eyepiece
Eyeguard
Bertrand lens ring
Centering nosepiece
CF Achromat P objective
(Stra in-free)
Vernier
Circular graduated stage
Achromat strain-free condenser
Orientation plate
Field lens
Brightness control dial
(including power switch)
Microflex clamp screw
Trinocular eyepiece tube "TP"
Optical path change-over knob
Eyepiece tube clamp screw
Analyzer knob
-----------
IIntermediate tube clamp screw
111'1
,
Substage clamp screw
Socket sleeve
Lamp vertical
centering ring
Lamp lateral
centering screw
Lamp input plug
Filter receptacle
Filter frame
~ ND16&GIFI
(NCB10. ND2.
Dust capLamp centering tool
Fig. 1

Analyzer rotation ring
Analyzer clamp screw
Intermediate tube "P"
Nosepiece clamp screw
45° click stop lever
Condenser focus knob
Coarse focus knob
Fine focus knob
Lamp housing
Lamp housing
clamp screw
Arm rest
-..
Compensator slot
1/4 A&tint plate
Nosepiece centering screw
Specimen clip
Condenser aperture
diaphragm control ring
Dia-polarizer
-------
Condenser clamp screw
Brightness indicator
Field diaphragm control ring
X-POL stand
Fig.2

II. ASSEMBLY
• To assemble the microscope, follow the procedure in the order given:
Substage
clamp scre,
Achromat strain-free condenser
Insert the condenser into the con-
denser carrier, facing the aperture
number plate toward the operator.
Fasten the clamp screw on the left
side of the carrier.
Circular graduated stage
Release the substage clamp screw using
a driver, and slide the substage on the
dovetail fitting. In such a position that
the top ends of the substage and the
dovetail are at the same level, fasten
the clamp screw.
CF eyepiece
Insert the eyepiece CFW 10XCM
into the right-hand sleeve, fitting
the pin of the eyepiece in the
right-hand notch of the sleeve.
Into the left-hand sleeve, insert
theCFW10X.
1/4 A&tint plate
Remove the screw by the side of
the 1/4 "A. plate of the 1/4 "A. & tint
plate and insert it into the com-
pensator slot of the intermediate
tube "P", facing the positioning
groove toward the operator side.
Reattach the removed screw.
Specimen clip
Place the clip on the stage
using holes on the stage sur-
face.
@
Aperture
number
plate
@Centering nosepiece
17 Lower the stage sufficiently.
Releasing the nosepiece clamp
screw on the left side of the
microscope arm, insert the nose-
piece. Making sure of positive
fitting of the pin on the micro-
scope arm into the nosepiece
groove, refasten the clamp screw.
@
@6CF Achromat P objective (Strain-free)
Mount the objectives on the nose-
piece in such positions that their
magnifying power increases as the
nosepiece is revolved clockwise.
@
lowest power
Leveling foot screw
For stable installation of the
microscope, manipulate the
adjusting screw at one foot on
the bottom of the microscope
base.
Power source voltage
Set the input voltage to the power
source voltage by means of the
change-over switch on the bottom
of the base.
If the illumination is found too
bright or unstable, when the switch is
turned ON, make adjustment of the
lowest voltage in the following way:
1) Turn the brightness control dial
to OFF.
2) Turn the lowest voltage adjusting
screw on the bottom of the
microscope base counterclockwise
to the limit, using a screw driver.
3) Turn the brightness control dial
to ON_ At this time, the lamp
voltage will be highest, immedi-
ately after lighting.
4) In this condition, gently turn the
lowest voltage adjustingsc;:ew
clockwise, to set the voltage to
nearly 4 on the ind icator.
Bottom of the Base
Bottom of the Base
~-------
~
Bottom of the Base

Eyepiece tube
Attach the eyepiece tube on the intermediate tube "P", fitting
the notch of the circular dovetail on the end of the clamp screw.
Fasten the clamp screw.
Intermed iate tube "P"
Attach the intermediate tube "P" on the arm of the X-POL stand,
fitting the notch of the circular dovetail on the end of the
clamp screw. Fasten the clamp screw.
Lamp input plug
Connect the plug to the
receptacle on the filter
receptacle's left side.
®Lamp housing
Insert the lamp
housing into the col-
lector lens, and fas-
ten the clamp screw
on the left side of
the housing.
@Filter
Place the filter into the filter
frame. After centering the lamp,
put the filter with the frame into
the filter receptacle. No frame is
provided for the diffuser.
®Power input plug
Connect the plug to the re-
ceptacle on the fi Iter recep-
tacle's right side.
Insert the lamp bulb with
its pins into the accepting
holes in the socket.
Note: Don't touch the
bulb surface directly
with the fi nger.
f3\ Halogen lamp bulb~ (12V-50W)
Filter frame
@
@
Eyepiece tube clamp screw
Socket sleeve
Insert the socket sleeve into
the lamp housing, and fasten
it firmly with the clamp screw.
Socket sleeve clamp screw
Fig. 3

III. PREPARATION
Lamp housing
clamp screw
Filament
image
Condenser
aperture
diaphragm
Socket sleeve
clamp screw
Lamp vertical
centering ring
Lamp lateral
centering screw
/
Fig. 6
7) Release the socket sleeve clamp screw (Fig.
6). Turning the lamp lateral centering screw
and vertical centering ring, bring the fila-
ment image to the center, as shown in
Fig.7.
Fig. 7
8) As shown in Fig. 8, put the diffuser, with
its matte surface faced toward the micro-
scope stand, into the filter receptacle
which is the closest to the microscope
stand.
Fig. 8
The above centering procedure should be car-
ried out, when replacing the lamp bulb.
Lamp housing
Lamp
centering
tool
1. Centering the Lamp
ND filter
1) Connect the power source cord to the
socket.
2) Turn the brightness control dial to switch
ON and adjust the voltage to 6 on the
indicator.
3) Place the specimen on the stage, and focus
on the specimen using lOx objective. In
this case, open the condenser aperture and
field diaphragms to the largest extent.
4) Roughly center the condenser lens using
lOX objective, following the procedures
given on P. 10- 6.
5) Put the lamp centering tool on the field
lens and onto the tool place a NO filter.
(Fig. 4)
Fig. 4
6) Stop down the condenser aperture dia-
phragm, release the lamp housing clamp
screw, and move the lamp housing back
and forth (Fig. 5), until a sharp image of
the lamp filament appears on the aperture
diaphragm surface, which can be seen by
the reflection from the NO filter.
Fig.5

Fig. 13
Vertical photo
tube: 86%
Observation Observation
tube: 100% tube: 14%
+-- --+
~OPtical path
change-over knob
Fig. 12
Fig.11
*Since the CF eyepieces are of high eye-
point type, it is not necessary for the user
putting on his spectacles to remove them.
Only fold down the eyeguard rubber.
(Fig.13)
4. Optical Path Change-over in the
Trinocular Eyepiece Tube "TP"
(Fig. 11)
5. Centeringthe Objectives
1) Place the specimen on the stage, and focus
on the specimen. Bring an appropriate tar-
get to the center of the cross lines in the
eyepiece.
2) Insert the centering tools in the centering
screws on the nosepiece. (F ig. 14)
2. Interpupillary DistanceAdjustment I
Place a specimen on the stage, and focus on the
specimen.
As shown in Fig. 9, adjust the interpupillary
distance, so that both the right and left view-
fields become one. '
Fig. 9
Fig. 10
3. Diopter Adjustment
Rotate the diopter ring on the eyepiece CFW
lOx CM until the cross lines are seen clear.
(Fig. 10)
(For b inocu lar observation)
1) Mount the specimen on the stage. Swing
the objective 10 x into position, and bring
the specimen image into focus looking into
the right-hand eyepiece.
2) Witll.Out manipulating the coarse-and-fine
focus knob, turn the diopter ring on the
left-hand eyepiece to focus on the speci-
men.
Fig. 14

3) Rotate the stage about 1800, and the target
is displaced from the center of the cross
lines. Move the objective using the center-
ing tools so that the center of the cross
Iines comes to one half position of the
displacement of the target. (F ig. 15)
Rotate
1800
Target-
A half
position
of the
displacement
Image of field
[~:a~~~~~~
~'\
)
,,>J
":/
Eyepiece
viewfield stop
Fig. 16
Fig. 15
• Repeat the above procedure two or three
times, and the rotation center of the stage
coincides with the cross Iines center .
• Carry out centering for each objective.
6. Centering the Condenser Lens
1) Close the field diaphragm in the micro-
scope base to its smallest size by means of
the field diaphragm control ring. Rotate
the condenser focus knob to move the
condenser vertically so that a sharp image
of the field diaphragm is formed on the
specimen surface.
2) Bring the field diaphragm image to the
center of the field of view by means of
the condenser centering screws.
(Fig. 16-1IJ)
3) Change over to the objective 40 x, and
adjust the field diaphragm so that the
image of the diaphragm is about the same
as the eyepiece viewfield stop, as shown in
Fig. 16-~. If not centered, use the con-
denser centering screws again.