Ocean Optics MFPF100-1 User manual

MultiFrequency Phase Fluorometer
Installation and Operation Manual
Document Number MFPF-00000-000-02-0606
Offices: Ocean Optics, Inc.
830 Douglas Ave., Dunedin, FL, USA 34698
Phone [phone]
Fax [phone]
8 a.m.– 8 p.m. (Mon-Thu), 8 a.m.– 6 p.m. (Fri) EST
Ocean Optics B.V. (Europe)
Ocean Optics B.V. (Europe)
Geograaf 24, 6921 EW DUIVEN, The Netherlands
Phone [phone]
F
ax 31-(0)26-3190505
E-mail: [email protected]m (General sales inquiries)
[email protected] (European sales inquiries)
[email protected] (Questions about orders)
[email protected] (Technical support)

Copyright © 2001-2006 Ocean Optics, Inc.
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means, electronic,
mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
This manual is sold as part of an order and subject to the condition that it shall not, by way of trade or otherwise, be lent, re-sold, hired out or
otherwise circulated without the prior consent of Ocean Optics, Inc. in any form of binding or cover other than that in which it is published.
Trademarks
All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their respective owners.
Limit of Liability
Ocean Optics has made every effort to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The
information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to
any loss or damages arising from the information contained in this manual.

Important Safety Notices
WARNINGS
1. Intense UV radiation is emitted from the MFPF LED. Do not look directly at the
LED output with the naked eye once the red cap is removed. To verify there is
power to the instrument, place a white piece of paper in front of LED 1.
2. Do not open the instrument case. High voltage is present. There are no
serviceable parts inside. Return the instrument to the factory for service.
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Important Safety Notices
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Table of Contents
Important Safety Notices ................................................................................................. i
About This Manual .......................................................................................................... iii
Document Purpose and Intended Audience.............................................................................. iii
Document Summary.................................................................................................................. iii
Product-Related Documentation ............................................................................................... iii
Chapter 1: Introduction ......................................................................1
MFPF System Overview.................................................................................................. 1
Applications ..................................................................................................................... 2
Models............................................................................................................................. 2
Shipment Components.................................................................................................... 3
Additional Recommended Equipment ............................................................................. 3
Chapter 2: Installation ........................................................................5
Overview ......................................................................................................................... 5
Software Installation ........................................................................................................ 5
Hardware Installation....................................................................................................... 7
Chapter 3: Configuration....................................................................11
Overview ......................................................................................................................... 11
Configuring the COM Port with the MFPF Software........................................................ 11
Configuring OOISensors Software .................................................................................. 13
Index .....................................................................................................17
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Table of Contents
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About This Manual
Document Purpose and Intended Audience
This document provides the users of the MultiFrequency Phase Fluorometer with instructions for setting
up, calibrating and performing experiments with their equipment.
Document Summary
Chapter Description
Chapter 1: Introduction Contains descriptive information about the MFPF unit. It also
provides a list of system requirements, interface options, and
shipment components.
Chapter 2: Installation Provides installation instructions.
Chapter 3: Configuration Contains steps to configure MFPF and OOISensors software
for use with the MFPF unit.
Product-Related Documentation
You can access documentation for Ocean Optics products by visiting our website at
http://www.oceanoptics.com. Select Technical →Operating Instructions, then choose the appropriate
document from the available drop-down lists. Or, use the Search by Model Number field at the bottom
of the web page.
•Detailed instructions for the OOISensors Software are located at:
http://www.oceanoptics.com/technical/NEW OOISensors FOXY Manual.pdf
Engineering-level documentation is located on our website at Technical →Engineering Docs.
You can also access operating instructions for Ocean Optics products from the Software and Technical
Resources CD that ships with the product.
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About This Manual
iv MFPF-00000-000-02-0606

Chapter 1
Introduction
MFPF System Overview
The MultiFrequency Phase Fluorometer (MFPF) is a flexible platform for measuring luminescence
lifetime, phase and intensity. The MFPF is a compact, self-contained, frequency-domain luminescence
monitor that uses LED excitation and photodiode detection with filter-based wavelength selection for
easy experimental set-up and control. Because the unit is self-contained, it is invariant to fiber bending
and stray light, and has a wide dynamic range of optical intensity as well as low optical and electronic
crosstalk, and low drift and phase noise. Therefore, the MFPF is especially useful for oxygen sensing
applications where sensitivity to drift is important and where sample set-ups must be left undisturbed for
long periods of time.
The MFPF is used with Ocean Optics’ Fiber Optic Oxygen (FOXY) Sensors and custom probes. FOXY
Fiber Optic Oxygen Sensors are low-power, portable devices that offer high sensitivity, reversibility, and
stability. Their small size is useful for remote monitoring. What's more, the thin film used in the probe
tips consumes no oxygen, allowing for continuous contact with the sample. FOXY Sensors offer other
key advantages: they're ideal for viscous samples and are immune to interference caused by pH change or
from changes in ionic strength, salinity, and biofouling. The MFPF unit supports the following Ocean
Optics sensors (and their respective frequencies):
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1: Introduction
•FOXY (40 kHz) – Ocean Optics’ standard oxygen sensors designed for monitoring oxygen
partial pressure in gas and aqueous solutions. FOXY is a fiber optic fluorescence probe with
proprietary oxygen-sensing thin-film coating on the tip.
•HIOXY (40 kHz) – Designed for monitoring oxygen partial pressure in nonaqueous vapors
and solutions. The sensor coating chemistry is compatible with oils, alcohols, and
hydrocarbon-based vapors and liquids.
•FOSPOR (5 kHz) – A new generation of highly sensitive sensor coating for monitoring traces
of oxygen in gas and liquids.
See http://www.oceanoptics.com/products/probes.asp for more information on probes
available from Ocean Optics.
The MFPF connects to a PC via USB connection and saves your data in an easy-to-use Microsoft Excel
format. The USB connection actually emulates the USB as a serial COM port. The MFPF can be
configured with two-channel LED excitation and detection, and modulation frequencies to 100 kHz. It
will give you lifetime measurements from 200 µsec down to 0.3 µsec. The on-board pressure transducer
measures atmospheric pressure or external pressure with a ¼-inch hose fitting.
The MFPF software manages the device and OOISensors software monitors its output via a DDE
software connection so that the MFPF software must be running in the background for the OOISensors to
work properly. MFPF software is Windows 2000/XP control software that measures over a wide dynamic
range of luminescence intensity, lifetime and phase, and allows you to select channels, the LED frequency
duty cycle and advanced instrument functions.
Applications
Typical applications include the following:
•Luminescent materials characterization
•Phase/lifetime-based sensor development
•Calibration of phase/lifetime-based sensors
•Stability and photodegradation studies
•Characterization of phase shift over frequency
•Oxygen consumption measurement on cell islet cultures
Models
Two models of the MFPF are available:
•MFPF100-1: Single-Channel MultiFrequency Phase Fluorometer. The single channel unit
comes complete with one thermistor.
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1: Introduction
•MFPF100-2: Two-Channel MultiFrequency Phase Fluorometer. The two-channel unit
includes two thermistors.
The thermistor option is used for temperature logging, calibration and temperature correction.
Shipment Components
The following equipment and information ships with the MFPF:
Single-Channel MFPF (MFPF100-1)
Includes one thermistor.
Or,
Two-Channel MFPF (MFPF100-2)
Includes two thermistors.
AC power supply
(Input AC100-240V, 50-60 HZ, 0.3A) (Output DC 6V 2A) (Model #EPAS-101W-06)
USB Cable
Software and Technical Resources CD
Each order ships with the Ocean Optics Software and Resources CD. This disc contains software,
operating instructions, and product information for all Ocean Optics software, spectrometers, and
MFPF spectroscopic accessories. You need Adobe Acrobat Reader version 6.0 or higher to view
these files. Ocean Optics includes the Adobe Acrobat Reader on the Software and Technical
Resources CD.
Ocean Optics software requires a password during the installation process. You can locate
passwords for the other software applications on the back of the Software and Technical
Resources CD package.
Packing List
The packing list is inside a plastic bag attached to the outside of the shipment box (the invoice
arrives separately). It lists all items in the order, the shipping and billing addresses, and any items
on back order.
Additional Recommended Equipment
FOXY, HIOXY or FOSPOR Oxygen Sensor Probe
FOXY-CAL, HIOXY-CAL, FOSPOR-CAL
This is an optional factory calibration service for environments from 0 to 80ºC.
MFPF Software
The MFPF software runs in the background to gather data from the MFPF unit for the main
interface, OOISensors software.
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1: Introduction
OOISensor Software
OOISensors is an advanced data acquisition and display program that provides a real-time
interface to a variety of signal processing functions for users of Windows
95/98/ME/NT/2000/XP. OOISensors software supports USB2000 and USB4000 Spectrometers,
and the MFPF unit.
Bifurcated Fibers
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Chapter 2
Installation
Overview
You must install the OOISensors software and the MFPF software applications prior to connecting the
MFPF to the computer to install the drivers required for the MFPF. If you do not install the software first,
the system will not properly recognize the MFPF.
Caution
Do not connect the MFPF unit included with the Fiber Optics Sensors System to the PC
prior to installing the OOISensors software.
Software Installation
Two types of software are used together to manage your MFPF instrument:
•OOISensors software – This is a 32-bit, advanced acquisition and display program that
provides a realtime interface to display and processing functions for use with Ocean Optics’
oxygen and pH sensors. OOISensors acquires data for use by the MFPF software to convert
data into concentration values, and save the data in spectral files and logs.
Two additional software packages are also included as part of the OOISensors installer to set
up the USB port as a serial COM port and to communicate with the MFPF unit.
•MFPF software –This is the application that controls and collects data from the MFPF unit.
The MFPF software is included as part of the OOISensors installation, so only one software installation
process is necessary.
►Procedure
To install OOISensors and MFPF software,
1. Close all other applications running on the PC.
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2: Installation
2. Start the software installation process.
Installing from CD:
a. Insert the CD containing the OOISensors and MFPF software.
b. Select the drive on your computer with the software CD.
c. Double-click on the Setup.exe. The installation wizard appears.
Installing from the Web:
a. Go to http://www.oceanoptics.com/technical/softwaredownloads.asp.
b. Right-click on OOISensors Oxygen Measurement Software and select Save Target As…
to download the executable to your machine.
c. Double-click on the downloaded file. The installation wizard appears.
3. Click the Next button at the Welcome screen. The Choose Destination Location screen appears.
4. Select a location for your software files. Click Next. The Backup Replaced Files screen appears.
5. Select Yes or No, depending on whether you want to create backup replacement files. If you select
Yes, choose a location for these files. Then click Next. The Select Program Manager Group screen
appears.
6. Select the name of the Program Manager group to which you want to add the OOISensor icons. Then
click Next. The Start Installation dialog box appears.
7. Click Next. The Installation Password dialog box appears. Enter the password for your OOISensors
software. Passwords are located on the back of the software CD jacket. The software begins
installing.
8. The MFPF dialog box appears asking you if you have MFPF hardware.
Notes
If a previous version of the MFPF software is detected on the computer, the installer will actually
remove it and not install the new version. To install it, either run Setup.exe from the
…\OOISensors\MFPF_installers\TauTheta_DDE_Server\ folder or just run the OOISensors
installer again. In other words, each time the OOISensors installation is executed, the MFPF
software toggles between being installed or uninstalled.
It is also possible to switch between a spectrometer and MFPF configuration in the software
configuration panel (see Configuring OOISensors Software for more information).
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1: Introduction
9. Click Yes. The Install Driver screen appears. Click Install to install the USB to UART Bridge
Controller Driver Set to the location shown on the screen, or browse to another location. A message
appears informing you that your installation is complete.
10. Click OK, then click Finish. You have now installed both OOISensors and MFPF software. You
must restart your computer to use the software.
Access MFPF software with the icon on your desktop. Access OOISensors software Start | All
Programs | Ocean Optics | OOISensors | OOISensors. If you attempt to start OOISensors software
without first starting the MFPF software, an error message appears.
Hardware Installation
WARNING
Ensure that the red plastic caps are covering the four SMA connectors on the front of the
MFPF unit. Intense UV radiation is emitted form the LEDS when the unit is powered-up.
Do NOT look directly at the LED output with the naked eye.
►Procedure
1. Unpack the equipment and verify that you have all the necessary components (see Shipment
Components and Additional Recommended Equipment).
2. Connect thermistor(s) to T1 and T2 (for Model MFPF100-2) connectors on front of unit.
3. Locate the 21-02 SMA Splice Bushing that came with the probe. This item is a 0.75" screw with
two female ends. Screw one end of the splice bushing into the SMA 905 connector on the end of
the probe.
4. Locate the bifurcated fiber that came with the system. This optical fiber assembly has a “Y”
shaped design. Connect the common end of the bifurcated fiber to the splice bushing/probe.
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2: Installation
5. Connect one arm (it doesn’t matter which one) of the bifurcated probe fiber to Ch1 LED and the
other arm to the Ch1 Detector on front of unit as shown below.
MFPF Unit Front Panel
6. Connect power cord from the power supply that came with your MFPF unit from back of unit to
an AC outlet. To check that the unit is receiving power, look for light glowing through the LED
#1 red cap, or place a piece of white paper in front of the LED if the red cap is not available. Do
NOT look directly at the light being emitted with the naked eye.
Caution
Use only the power supply that came with your MFPF unit. Using a different power supply
could damage your equipment.
7. Connect the MFPF unit to your computer using the USB cable.
Note
The current version of the MFPF uses a USB connection with an emulated RS232 port. Before
starting the MFPF software application, you must identify the COM port number of the emulated
RS232/USB port.
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1: Introduction
MFPF Unit Rear Panel
Once you have installed both the hardware and the software, you must configure your system (see
Chapter 3: Configuration).
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2: Installation
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Chapter 3
Configuration
Overview
Once the software has been installed, the system has been rebooted, and the hardware has been connected
to the computer, you must configure the software.
Configuring the COM Port with the MFPF
Software
The MFPF unit uses a USB connection with an emulated RS232 port. Before you are permitted to start
the software, you are asked to identify the COM port number of the emulated RS232/USB port.
►Procedure
To select the COM port for the emulated RS232/USB port,
1. Click the Windows Start icon.
2. Select Control Panel | System to display the System Properties window.
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3: Configuration
3. Select the Hardware tab.
4. Click Device Manager.
5. Scroll down and expand the Ports (COM & LPT) list item.
6. Note the port number assigned to the CP210x USB to UART device.
7. Ensure that this is the COM port selected on the MFPF software Set Up screen.
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