Ocean Optics OCEAN FX-UV-VIS User manual

Ocean FX Miniature Spectrometer
User Manual
Amy to r
For Product: OCEAN FX
Document: 226-00000-000-01
Version: 1.3

226-00000-000-01 i
AMERICAS & WORLD HEADQUARTERS
Phone: [phone]
Fax: [phone]
Sales: [email protected]m
Orders: [email protected]m
Support: [e-mail]
EUROPE, MIDDLE EAST & AFRICA
Phone: [phone]
Fax: [phone]
Email: [email protected]
Germany : [phone]
UK : [phone]
France : [phone]
ASIA
Phone: [phone]
Fax: [phone]
Email: [email protected]m
Japan & Korea: [phone]
www.oceanoptics.com
Copyright © 2017 Ocean Optics, Inc.
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, by any means,
electronic, mechanical, photocopying, recording, or otherwise, without written permission from Ocean Optics, Inc.
Trademarks
All products and services herein are the trademarks, service marks, registered trademarks or registered service marks of their
respective owners.
Limit of Liability
Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The
information provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity
with respect to any loss or damages arising from the information contained in this manual.
Ocean Optics, Inc.
830 Douglas Ave.
Dunedin, FL 34698
USA
Manufacturing & Logistics
4301 Metric Dr.
Winter Park, FL 32792
USA
Ocean Optics Asia
666 Gubei Road
Kirin Tower Suite 601B
Changning District
Shanghai
PRC, 200336
www.oceanoptics.cn
Sales & Support
Geograaf 24
6921 EW Duiven
The Netherlands
Manufacturing & Logistics
Maybachstrasse 11
73760 Ostfildern
Germany

226-00000-000-01 ii
Table of Contents
Compliance .................................................................................................................... vi
Warnings & Cautions ..................................................................................................... viii
About This Manual ......................................................................................................... ix
Document Summary .................................................................................................................. ix
Product-Related Support and Documentation ........................................................................... x
Warranty ........................................................................................................................ xi
Chapter 1 ............................................................................................ 1
Introduction ........................................................................................ 1
Product Introduction ....................................................................................................... 1
Product Features ....................................................................................................................... 2
Typical Applications ................................................................................................................... 3
Product Versions ........................................................................................................................ 5
Chapter 2 ............................................................................................ 6
How the Ocean FX Spectrometer Works .......................................... 6
Overview ........................................................................................................................ 6
Chapter 3 ............................................................................................ 11
Installation and Setup ........................................................................ 11
What’s In the Box ........................................................................................................... 11
Ocean FX Installation ..................................................................................................... 12
Software Installation ................................................................................................................... 12
About OceanView ...................................................................................................................... 13
Initial Configuration .................................................................................................................... 13
Configuration for USB or Ethernet Connection .......................................................................... 14
Configuration for WiFi Connection ............................................................................................. 19
Power Sequences ...................................................................................................................... 25
Experiment Setup ...................................................................................................................... 26
Ocean FX Indicator Lights ......................................................................................................... 27
Interchangeable Slits ................................................................................................................. 27
Changing the Interchangeable Slits ........................................................................................... 27
Accessories .................................................................................................................... 29
Cables and Connectors ............................................................................................................. 29
Breakout Box (HR4-BREAKOUT) .............................................................................................. 29
Light Sources, Cuvette Holders and Other Accessories ........................................................... 29
Measurement Techniques – Typical Set-ups .................................................................. 30
Common UV-Vis Applications .................................................................................................... 30
Reflectance & Transmission ...................................................................................................... 31

About This Manual
226-00000-000-01 iii
Common UV-Vis Reflectance Applications ................................................................................ 31
Common UV-Vis Transmission Applications ............................................................................. 31
Fluorescence.............................................................................................................................. 32
Common Fluorescence Applications ......................................................................................... 32
Irradiance ................................................................................................................................... 33
33
Chapter 4 ............................................................................................ 35
Ocean FX Operation with OceanView .............................................. 35
Overview ........................................................................................................................ 35
Launch OceanView ........................................................................................................ 35
OceanView Main Screen ................................................................................................ 36
Connect the Ocean FX in OceanView ....................................................................................... 37
Set Acquisition Parameters ........................................................................................................ 38
Continuous and Single Acquisitions .......................................................................................... 38
Save Data .................................................................................................................................. 39
Saved Data Panel ...................................................................................................................... 39
Projects and Methods ................................................................................................................ 40
Spectroscopy Application Wizards ............................................................................................ 40
Dark and Reference Measurements .......................................................................................... 41
Schematic View ......................................................................................................................... 41
Chapter 5 ............................................................................................ 43
Troubleshooting ................................................................................ 43
Overview ........................................................................................................................ 43
Microsoft Windows Operating Systems ..................................................................................... 45
Apple Mac OSX Operating Systems .......................................................................................... 45
Linux Operating Systems ........................................................................................................... 45
Chapter 6 ............................................................................................ 48
Technical Specifications ................................................................... 48
Mechanical Diagram ...................................................................................................... 48
Absolute Maximum Ratings ............................................................................................ 49
Performance Specifications ............................................................................................ 49
Accessory Connectors ................................................................................................... 52
DD4 Accessory Connector ......................................................................................................... 52
I2C .............................................................................................................................................. 54
DB15 Connector Cable (OCEAN FX-CBL-DD4P-DB15P) ........................................................ 54
Chapter 7 ............................................................................................ 56
Calibration .......................................................................................... 56

About This Manual
226-00000-000-01 iv
Overview ........................................................................................................................ 56
Wavelength Calibration .................................................................................................. 56
About Wavelength Calibration ................................................................................................... 56
Calibrating the Spectrometer Wavelength ................................................................................. 57
Preparing for Calibration ............................................................................................................ 57
Calibrating the Wavelength of the Spectrometer ....................................................................... 57
Irradiance Calibrations ................................................................................................... 59

About This Manual
226-00000-000-01 v
Tables
Table 1 .......................................................................................................................................... ix
Table 2 ........................................................................................................................................... 2
Table 3 ........................................................................................................................................... 4
Table 4 ........................................................................................................................................... 5
Table 5 ........................................................................................................................................... 5
Table 6 ........................................................................................................................................... 7
Table 7 ........................................................................................................................................... 8
Table 8 ......................................................................................................................................... 12
Table 9 ......................................................................................................................................... 27
Table 10 ....................................................................................................................................... 49
Table 11 ....................................................................................................................................... 49
Table 12 ....................................................................................................................................... 50
Table 13 ....................................................................................................................................... 51
Table 14 ....................................................................................................................................... 51
Table 15 ....................................................................................................................................... 53
Table 16 ....................................................................................................................................... 55

About This Manual
226-00000-000-01 vi
Compliance
WARNING
This is a FCC Class A product. In a domestic environment, this
product may cause radio interference in which case the user may
be required to take adequate measures.
FCC COMPLIANCE
This equipment has been tested and found to comply with the limits for a
Class A digital device, pursuant to Part 15 of the FCC Rules. These limits
are designed to provide reasonable protection against harmful interference
when the equipment is operated in a commercial environment. This
equipment generates uses and can radiate radio frequency energy and, if
not installed and used in accordance with the instruction manual, may
cause harmful interference to radio communications. Operation of this
equipment in a residential area is likely to cause harmful interference in
which the user will be required to correct the interference at his own
expense.
WARNING
The authority to operate this equipment is conditioned by the
requirement that no modifications will be made to the equipment
unless the changes or modifications are expressly approved by the
manufacturer.
WEEE COMPLIANCE
The WEEE symbol on the product indicates that the product must not be
disposed of with normal household waste. Instead, such marked waste
equipment must be disposed of by arranging to return to a designated
collection point for the recycling of waste electrical and electronic
equipment. Separating and recycling this waste equipment at the time of
disposal will help to conserve natural resources and ensure that the
equipment is recycled in a manner that protects human health and the
environment.

About This Manual
226-00000-000-01 vii
TUV CERTIFICATION
This device has been tested and complies with the following standards:
EN 61326-1:2013
CISPR 11:2009.A1:2010
CAN ICES-003, issue 6
EMC 2004/108/EC
RoHS-compliant
ISO COMPLIANCE
Ocean Optics, the industry leader in miniature photonics, has been certified for ISO 9001:2008
certification applicable to the design and manufacture of electro-optical equipment since 2009.

About This Manual
226-00000-000-01 viii
Warnings & Cautions
Warnings
This device may cause radio interference or may disrupt the operation of nearby
equipment. It may be necessary to take mitigation measures such as re-orienting,
relocating or shielding the location.
Cautions
Caution: Do not let contaminants get into the bench. Keep the protective cap on the slit
aperture when not connected to an accessory, probe or fiber.
Caution: Only change the slit aperture in a clean environment where contaminants including
dust cannot enter the bench during the procedure.
Caution: Substitution of a component or accessory different from that supplied may result in
measurement error, equipment damage, increased emissions or decreased immunity.
Caution: Repairs should be undertaken only by personnel trained or authorized by Ocean
Optics. The device does not contain any user serviceable parts.
Caution: Do not immerse the device in any fluid, place fluids on top of or attempt to clean with
liquid detergents or cleaning agents. This may cause an electrical hazard. Do not
use if accidental wetting occurs.
Caution: Do not remove any covers. Doing so may increase the risk of electrical shock or
compromise the integrity of the optical components.
Caution: Do not gas sterilize or autoclave this device.
Caution: Consult local codes and ordinances for proper disposal of equipment and other
consumable goods.
Caution: The device and/or accessories may not operate correctly if used or stored outside the
relevant temperature and humidity ranges described in the Technical Specifications.
Caution: Do not use if device is dropped and/or damaged. Have an authorized service
representative check the device before using again.
Caution: Be sure to install any software BEFORE connecting the spectrometer to your PC. The
software installs the drivers required for spectrometer installation. If you do not install
the software first, the system will not properly recognize the spectrometer.
Caution: To ensure reliable operation, it is recommended that the power supply be attached
prior to inserting the USB connector.
Caution: The user of this spectrometer shall have the sole responsibility for any malfunction
which results from improper use, faulty maintenance, improper repair, damage or
alteration by anyone other than Ocean Optics or their authorized service personnel.

About This Manual
226-00000-000-01 ix
About This Manual
Document Summary
Chapter Description
Chapter 1: Introduction Introduces the product features. Contains descriptive
information about the Ocean FX Spectrometer. It also
provides a list of system requirements, typical applications,
and product versions.
Chapter 2: How the Ocean FX
Spectrometer Works
Describes how the Ocean FX operates, illustrating the various
parts and functions.
Chapter 3: Installation and Setup Provides installation instructions, including how to set up the
Ocean FX with OceanView. Also includes package contents
and typical set-ups for different measurement techniques.
Chapter 4: Ocean FX Operation with
OceanView
Describes how to use the Ocean FX with OceanView
software, including how to connect, acquire, save and other
basic features.
Chapter 5: Troubleshooting Contains recommended steps to isolate and correct common
problems.
Chapter 6: Technical Specifications Contains technical specifications and connector pinouts for the
Ocean FX Spectrometer.
Chapter 7: Calibration Provides information for calibrating the Ocean FX
Spectrometer.
Table 1

About This Manual
226-00000-000-01 x
ACRONYMS & SYMBOLS
I2C
Intelligent Interface Controller
MISO
Master Input, Slave Output
IEEE Institute of Electronic and Electrical Engineers
MOSI
Master Output, Slave Input
Rx Receive
SPI
Serial P
eripheral Interface
Tx
Transmit
USB Universal Serial Bus
Vusb
Voltage, USB
WiFi Wireless Fidelity
FWHM
Full Width Half Maxi
mum
–
optical resolution units
CMOS
Complementary metal oxide semiconductor
–
type of detector
CCD Charged Coupled Device - type of detector
TEC
Therm
oelectric
Coo
ling
Product-Related Support and Documentation
You can access product documentation for Ocean Optics products by visiting our website at
http://www.oceanoptics.com. Go to the Ocean FX product page for general product details,
specifications and application notes. Additional technical documents and programs can be
accessed through the Support tab which includes software downloads, getting started guidance,
frequently asked questions and technical documents for topics including OceanView Software,
light sources, electronic accessories, sampling accessories, fibers & probes, external triggering,
changing the slit size and device driver guidance.
Ocean Optics offers a Glossary of spectroscopy terms to help you further understand your
state-of-the-art products and how they function located in the Knowledge section which also
offers example setups, application blogs, videos through SpectroscopyTV.com and other ways
of learning about spectroscopy.

About This Manual
226-00000-000-01 xi
Warranty
Our 3-Year Warranty covers Ocean Optics miniature fiber-optic spectrometers, spectral
sensors, light sources and sampling accessories – regardless of the application – from defects
in materials and workmanship from the date of purchase. It also covers fibers and probes for a
full 12 months.
The warranty covers parts and labor needed to repair manufacturing defects that occur during
the warranty period. We also will cover the costs of shipping warranty-related repairs from our
customers to Ocean Optics and from us to our customers. Repairs and upgrades are covered
for manufacturing defects for 6 months from the date of purchase from Ocean Optics.
Normal wear, scratching and cosmetic damage not affecting the performance, bulbs, batteries,
consumables and vendor items are not covered by this Ocean Optics limited warranty. For
vendor items, the manufacturer’s warranty terms are in force and vary from product to product.
These causes are not covered by this warranty: damage caused by accident, misuse – such as
using an incorrect power supply or power current – abuse, product modification or neglect;
damage occurring during shipment; damage resulting from the performance of repairs by
someone not authorized by Ocean Optics; damage caused by installation of parts that do not
conform to Ocean Optics specifications; units not used for their intended purpose; and any
claims made based on misrepresentations of the seller and costs associated with installation of
the unit.
Ocean Optics’ liability is limited to the repair or the replacement, at our option, of any defective
item and shall not include incidental or consequential damages whatsoever. Ocean Optics
reserves the right to replace a discontinued model with a functionally comparable model.
If you require warranty service, please contact the Customer Service Department of Ocean
Optics at +1.727.733.2447 or fill out a Return Merchandise Authorization (RMA) form located in
the Support section of our website.

226-00000-000-01 1
Chapter 1
Introduction
Product Introduction
The Ocean FX is a miniature modular spectrometer with enhanced connectivity and onboard
processing that is based upon Ocean Optics’ Cross Czerny-Turner design. Ocean FX is built
using manufacturing techniques that help deliver high thermal stability and low unit to unit
variation without compromising the flexibility and configurability that are the hallmark of the
design. Features such as interchangeable slits, indicator Lights and simpler device connectors
deliver more freedom and less frustration. In addition, Ocean FX now utilizes a USB 3.0
connecter for increased speed and also has 2 new methods of interfacing with the
spectrometer: Gigabit Ethernet and 802.11a/b/g/n WiFi.
Ocean FX Spectrometer

1: Introduction
226-00000-000-01 2
Product Features
Fast Acquisition Speed Integration times down to 10µs, acquire and process more
spectral data in less time for faster, more reliable answers
Sensitive Responsive from 190 – 1100 nm with great sensitivity in the
UV and NIR
Multiple Configurations Incredibly configurable, with millions of configurations across
the wavelength range 190-1100nm
Large Onboard Memory Onboard buffering stores up to 50,000 spectra so you will not
miss a single data point
Spectra Timestamping All spectra are timestamped
Expanded Connectivity Gigabit Ethernet, WiFi, USB, RS-232 and SPI for easy
integration into almost any system as well as 8 GPIO for
connection to external devices
On-the-fly Slit Modifications User-interchangeable slit allows quick changes to resolution
and throughput allowing one spectrometer to perform multiple
types of experiments such as absorbance and fluorescence.
High Thermal Stability Allows for accurate and repeatable measurements in
demanding environments
Portable Robust Design Compact, rugged and lightweight for use in the lab or in
remote applications
Table 2

1: Introduction
226-00000-000-01 3
Typical Applications
Application Area Examples
Light Laser LED
Laser Characterization
LED Measurement
Light Metrology Measurement
Research and Education
Applied Research
Basic Research
Teaching Labs for Physics, Chemistry, Biomed
Life Sciences
Biotechnology
Medical Diagnostics
Protein and Nucleic Acid Analysis
Materials Identification
Biomaterial Analysis
Metallurgical Analysis
Polymer Analysis
Semiconductor Materials Analysis
Semiconductors Processing and Thin Film
Metrology
Plasma Monitoring
Process Endpoint Detection
Thickness Measurement
Farm to Table Technologies
Agricultural Measurements and Monitoring
Food and Beverage Quality Control
Food Safety

1: Introduction
226-00000-000-01 4
Application Area Examples
Energy Technologies
Biofuels Analysis
Mining and Exploration
Oil and Petroleum Analysis
Photovoltaic Analysis
Solar Simulators
Anti-Counterfeit
Testing and Qualification
Product Identification and Authentication
Quality Control and Process Monitoring
Defect Identification
Raw Material Inspection
Verification Testing
Environmental Monitoring
Air and Water Quality Analysis
Remote Sensing
Volcanic Research
Table 3
You can find more information about applications of UV-Vis spectroscopy and the Ocean FX at
www.oceanoptics.com.

1: Introduction
226-00000-000-01 5
Specifications Summary
Spectral range 200 - 1100 nm (configurable within this range)
Optical resolution 2.39 pixels (FWHM)
SNR (single scan) 290:1
Dynamic range (single scan) 5000:1
Integration time 10 µs – 10 seconds
Scan rate (maximum) 4500 scans/second
Thermal stability 0.11 pixels/ C
Entrance slit 5, 10, 25, 50, 100 or 200 µm width slits
Input fiber connector SMA 905 or FC
Table 4
Product Versions
Many variants of the Ocean FX Spectrometer exist. Ocean Optics offers both preconfigured
units as well as custom-configured units, enabling you to order a customized spectrometer
optimized for your application. You can determine spectrometer details by looking at the product
code located on the bottom of your spectrometer.
OCEAN FX Preconfigured Models
Model Range
(nm)
Slit
(μm)
Optical
Resolution
(nm)
Grating # Lens
General
Purpose
OCEAN FX-UV-VIS
200-850 25 1.5 1
none
OCEAN FX-UV-VIS-ES L2
OCEAN FX-VIS-NIR
350-1000 25 1.5 3
none
OCEAN FX-VIS-NIR-ES L2
Extended
Range
OCEAN FX-XR1
200-1025 25 2 31
none
OCEAN FX-XR1-ES L2
Table 5
For more information and specifications on preconfigured models, see www.oceanoptics.com.

226-00000-000-01 6
Chapter 2
How the Ocean FX Spectrometer
Works
Overview
This section provides an overview of the Ocean FX spectrometer and how it works from light
entering the slit to the communication of the spectrum to a connected device. It also provides an
overview of all the different possible configurations that are possible, designed to help you
optimize your spectrometer for specific applications.
You’ll find more useful information, including a glossary of spectroscopy and spectrometer
terms, on our website at www.oceanoptics.com.
Ocean FX Open Bench
1. Fiber Optic Connector: Light from a fiber enters the optical bench through the SMA
905 Connector. The SMA 905 bulkhead provides a precise location for the end of the