Okolab CO2-O2-UNIT-BL User manual

1
CO2-O2-UNIT-BL [0-10; 1-18]
Gas Controller
Manual
IST1084_REV01
SV 3.12.3.3048

2
This page was left Blank

3
Index
1PREFACE .....................................................................................................................................................................................4
2SYMBOL DESCRIPTION ..........................................................................................................................................................5
SYMBOLS USED IN THIS MANUAL...............................................................................................................................................................5
SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................5
3SAFETY NOTES..........................................................................................................................................................................6
4SUPPLIED EQUIPMENT..........................................................................................................................................................8
5GAS SUPPLIED REQUIREMENTS .........................................................................................................................................9
6COMPATIBILITY.......................................................................................................................................................................9
7EQUIPMENT DESCRIPTION ............................................................................................................................................... 11
8INSTALLATION ...................................................................................................................................................................... 12
SETTING UP CO2-O2-UNIT-BL [0-10; 1-18].................................................................................................................................12
HOW TO ASSEMBLE CO2-O2-UNIT-BL [0-10; 1-18] WITH A BOLD LINE TCONTROLLER ....................................................19
9USER INTERFACE .................................................................................................................................................................. 21
HOME PAGE................................................................................................................................................................................................21
9.1.1 How to enter the set point ......................................................................................................................................................22
9.1.1.1 How to operate without N2.......................................................................................................................................................................... 22
CONTROLLER STATUS:COLOURS LED AND MEANING..........................................................................................................................24
SETTINGS....................................................................................................................................................................................................25
9.3.1 Gas......................................................................................................................................................................................................25
9.3.1.1 Air Source............................................................................................................................................................................................................25
9.3.1.2 Calibrations........................................................................................................................................................................................................ 27
9.3.1.2.1 Offset (with exthernal meter).............................................................................................................................................................27
9.3.1.2.2 Sensors (with calibration gas)........................................................................................................................................................... 29
9.3.1.2.2.1 View ......................................................................................................................................................................................................29
9.3.1.2.2.2 Span Settings .................................................................................................................................................................................... 30
9.3.1.2.2.3 Factory Reset ....................................................................................................................................................................................30
9.3.1.2.2.4 Start Span........................................................................................................................................................................................... 31
9.3.1.2.2.5 Start Zero............................................................................................................................................................................................31
9.3.1.2.2.6 Start Air............................................................................................................................................................................................... 32
9.3.1.2.3 Factory Reset.............................................................................................................................................................................................33
9.3.1.3 Flowrates............................................................................................................................................................................................................. 33
9.3.1.4 Gas cycles............................................................................................................................................................................................................. 35
9.3.1.5 Status ....................................................................................................................................................................................................................37
9.3.1.6 Digital Input.......................................................................................................................................................................................................37
9.3.2 Touch Screen configuration ...................................................................................................................................................38
9.3.2.1 Touch Screen Options..................................................................................................................................................................................... 38
9.3.2.2 Touch Screen Brightness ..............................................................................................................................................................................39
9.3.2.3 Touch Screen Visual Effects –icon and glance mode.......................................................................................................................39
9.3.2.4 Date & Time........................................................................................................................................................................................................ 40
9.3.2.5 Alarms...................................................................................................................................................................................................................40
9.3.2.6 Data Logging .....................................................................................................................................................................................................42
OVERVIEW..................................................................................................................................................................................................45
ICON AND GLANCE MODE VIEW.............................................................................................................................................................46
INFO PAGE ..................................................................................................................................................................................................46
10 TOUCH SCREEN CALIBRATION .................................................................................................................................... 48
11 CLEANING & MAINTENANCE......................................................................................................................................... 49
12 SUPPORT ............................................................................................................................................................................. 50
13 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 51
14 TROUBLESHOOTING........................................................................................................................................................ 52
15 FIGURE LIST........................................................................................................................................................................ 53
16 MANUAL REVISION TABLE ............................................................................................................................................ 55

4
1Preface
CO2-O2-UNIT-BL [0-10; 1-18] is a Digital CO2/O2Controller that mixes CO2, N2 and Air to the desired
concentration in the CO2 range 0-10% and O2 range 1-18%.
It features a long life Non Dispersive Infrared (NDIR) dual wave length CO2 detector and a long life O2
optical sensor to ensure a high accuracy on the gas composition.
CO2-O2-UNIT-BL [0-10; 1-18] is operated via the OKO-TOUCH touch screen control panel (to be ordered
separately). OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition data logging is possible via SMART-BOX, via DATA-LOG Okolab software and via following
third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN
Black software is available upon request.
Okolab Bold Line controllers are also capable to receive TTL input (requires to order separately TTL-
CABLE).
CO2-O2-UNIT-BL [0-10; 1-18] allows easily operating without N2 by setting the maximum Oxygen % value
from the Touch Screen.
CO2-O2-UNIT-BL [0-10; 1-18] connects to any Okolab Bold Line T controller. In this case, a single OKO-
TOUCH operates both, the Bold Line T controller and CO2-O2-UNIT-BL [0-10; 1-18].
CO2-O2-UNIT-BL [0-10; 1-18] is also compatible with Okolab Active Humidity Controller to control output
gas Relative Humidity and with Okolab air pump OKO-PUMP-BL, which enables to use background air as a
convenient alternative to the compressed air.

5
2Symbol description
This paragraph describes the symbols used in this manual and on the product label.
Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully use the instrument.
Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.
This symbol indicates the product production date.
This symbol indicates the Manufacturer data.
This symbol warns you to read the user manual before starting the device.
This symbol indicates the protection degree against ingress of solids or liquids inside the
product.
IP 30

6
3Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.
−The equipment must only be used as intended and as described in this Manual.
−Equipment should be operated only by technically qualified personnel.
−Do not start up the equipment if some of its parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Transport the equipment with care.
−Equipment and its internal parts can be damaged by dropping and by shock.
−Not following these instructions can result in damage or breakdown of the device and its
accessories.
−The products labels can be found on the bottom panel of the Main Box.
−Do not disassemble any part of the system.
−Do not use a volatile solvent such as paint thinner to clean the instrument, because
deformation or discoloration may occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Do not exceed voltage indicated in this manual and on the product label.
−Avoid excessive induction noise, static electricity and magnetic fields.
−Do not expose this instrument to rain or moisture.
−Do NOT go in close contact with or breathe any gas stream whose composition is different
from that of ambient air.
−Prevent throttling and kinking of tubing.
−Check tubing time to time for possible material usage.
−Check that all tubing are well inserted into the connectors so they cannot slip off
−This device is not designed for use for medical applications.
−Install safety valves and adequate pressure regulators on gas lines before CO2-O2-UNIT-
BL [0-10; 1-18] input connectors.
−Power cord of unit should be unplugged from electrical outlet when left unused for a long
period of time.
−PRESSURIZED GAS. Secure all connections with hose clamps. Never exceed the input
pressure limit of 1.5 bar. Bleed all lines before disconnecting. Wear safety goggles if needed.
If pressure regulators are not within sight and reach, make sure at least one shut off valve
is within reach.
−LOW OXYGEN ATMOSPHERES. Never enter a room or enclosure which has a low oxygen
atmosphere because of severe danger of suffocation. Only operate in well-ventilated room.
A small amount for carbon dioxide gas leaks continuously out of the instrument and should
never be allowed to build up in the room.
−VENTILATION. Unit should be situated so that its location or position does not interfere
with proper ventilation. Neither the gas mixer nor stream destinations should be in poorly
ventilated areas.
−Unit should be situated away from heat sources such as open flames, radiators, heat
registers, stoves, or other appliances or processes that produce heat.

7
−Do not start up the equipment if the supply cable is damaged.
−Connect the equipment only to grounded mains power socket.
−Do not disconnect cables while in operation.
−Do not open the unit. Do not remove cover or back.
−Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric
shock, fire or malfunction.
−No user serviceable parts inside.
−Unit should never be used where it can fall or be pushed into water.
International caution symbol marks this device. It is important to read the “Safety
Notes” before installing, using and commissioning this device, as the notes contain
important information relating to safety and EMC. Not following these instructions
can result in damage or breakdown of the device and its accessories
We reserve the right to make technical variations.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE
OF ANY PRODUCT.

8
4Supplied equipment
1. CO2-O2-UNIT-BL [0-10; 1-18], main box.
2. PTFE filter (x2). One to be installed on the rear of CO2-O2-UNIT-BL [0-10; 1-18] and one as spare
replacement.
3. TUBE-C (x1). Polyurethane 130cm long blue rigid tube 6mm OD with connected a 4mm ID Silicon transparent
tube 150 cm long plus PTFE filter in the middle. Use TUBE C to connect the CO2/O2 Controller to your device
or to the humidifier, if it is present.
Note ►If you purchased CO2-O2-UNIT-BL [0-10; 1-18] with H201-T-UNIT or H101-T-UNIT, you receive the
TUBE-Y, Polyurethane 1m long blue rigid tube 6mm OD, connected to a Silicon 15cm long transparent tube
4mm ID plus PTFE filter in the middle, connected to a Polyurethane 1m long blue tube 6mm OD. Use TUBE-Y
to connect CO2-O2-UNIT-BL [0-10; 1-18] to the cage incubator.
4. TUBE-A (x3). Rigid tubes, Polyurethane 3m long 6mm OD each. Use TUBE A to connect N2, CO2 and Air supply
to the corresponding input ports (N2, CO2, Air) on the rear panel of CO2-O2-UNIT-BL [0-10; 1-18].
5. Pressure Gauge for CO2 and N2+ regulator + assembly stirrup (x2). Install pressure gauges between the
pure CO2 and N2 tank pressure regulator and CO2-O2-UNIT-BL [0-10; 1-18] input port.
6. 24V-DC Power Adapter (x1).
4
1
362
5
To Air supply
To CO2 supply
To N2 supply
Figure 1. CO2-O2-UNIT-BL [0-10; 1-18] BL - components.

9
5GAS supplied requirements
−CO2. CO2 must be available at 1.4 barg, with a connector for 6mm OD rigid silicon tube. Gas source must
be Standard Purity CO2 (coded as 4.5 that means 99.995 % of CO2) and humidity-free gas.
−N2. N2 must be available at 1.4 barg, with a connector for 6mm OD rigid silicon tube. Gas source must be
Standard Purity N2 (coded as 4.5 that means 99.995 % of N2) and humidity-free gas.
−Air. Air must be available at 1.4 barg, with a connector for 6mm OD rigid silicon tube.
Note ►An alternative to the compressed air, OKO-AIR-PUMP-BL allows to use background Air.
6Compatibility
CO2-O2-UNIT-BL [0-10; 1-18] is compatible with:
•OKO-TOUCH (required), including:
−Touch Screen Interface
−#1 RS232 Serial cable. To connect the Touch Screen to the unit.
−MINI-USB-OTG cable. To connect a USB pen to the OKO-TOUCH for data download.
•OKO-AIR-PUMP-BL
•H101-BASIC-BL
•H101-CRYO-BL
•H201-T-UNIT-BL
•H301-T-UNIIT-BL-PLUS
•HM-ACTIVE
•H101-HM
•HM-VF
OKO-AIR-PUMP-BL
HM-ACTIVE
H301-T-UNIT-BL OKO-TOUCH
Figure 2. CO2-O2-UNIT BL [0-10; 1-18] within a typical H301 configuration.

10
•DATA-LOG. Software used to log, view, and store data from a PC. It may be used to change parameters of
the controllers via PC. Data can be exported in .xlsx format.
Figure 3. DATA LOG software.
•SM-BL Smart Box. To store data, to operate the controllers via web/smartphone/tablet and to allow
remote assistance for service.

11
7Equipment Description
Figure 4 illustrates CO2-O2-UNIT-BL [0-10; 1-18] front panel:
1. Status Led.
2. RS232 connector. Connector for OKO-TOUCH.
12
Figure 4. CO2-O2-UNIT-BL [0-10; 1-18] front panel.
A detailed description of CO2-O2-UNIT-BL [0-10; 1-18] rear is provided in the following list, which refers
to the pointers in Figure 5:
1. Filter Out. To connect the PTFE filter.
2. Filter In. To connect the PTFE filter.
3. Gas Output.
4. Power Input.
5. OKO-AIR-PUMP-BL connector. Leave unplugged if the system is not equipped with OKO-AIR-PUMP-BL.
6. HM-ACTIVE connector. To connect the HM-ACTIVE active humidity controller.
7. GPIO (General Purpose Input Output) connector.
8. USB connector.
9. Air. Push to fit Input Connector for 6 mm O.D. rigid tubing carrying air from air source.
10. Pure N2. Push to fit Input Connector for 6 mm O.D. rigid tubing.
11. Pure CO2. Push to fit Input Connector for 6 mm O.D. rigid tubing.

12
1 2 3
4 5 6 7 8 9 1110
Figure 5. CO2-O2-UNIT-BL [0-10; 1-18] rear panel.
8Installation
The following paragraphs will illustrate how to install and to use CO2-O2-UNIT-BL [0-10; 1-18].
Setting up CO2-O2-UNIT-BL [0-10; 1-18].
The following instructions shall give you the possibility to install the unit quickly. For safe operation
of the unit it is absolutely necessary to read carefully all the instructions and safety notes.
1. Install the pressure Gauge for the CO2 and N2 lines between the CO2/N2 tanks and CO2-O2-UNIT-BL [0-
10; 1-18]. Follow the arrow on the rear of the Pressure Gauge for the correct Gas In-Out (see Figure 6).

13
GAS IN GAS OUT
Figure 6. Input and Output gas ports. See symbol on the rear of Pressure Gauge
On the bottom of the Pressure Gauge there is an automatic purging valve. Close it before operating the
Pressure Gauge. To close the purging valve, looking at the regulator vertically from the top knob, pull it down and
rotate it counterclockwise (see A and B in Figure 7). Now you can operate the Pressure Gauge.
Purging valve
A B
Figure 7. How to close the purging valve of the Pressure Gauge
To close the Pressure Gauge, pull the knob up and rotate it counterclockwise. Push the knob down to lock
it. Open valve on the gas supply tank. Adjust Pressure Gauge by pulling the top knob up and rotating it clockwise
until the pressure reaches the desired value (see Figure 8), then push the top knob down to lock it.

14
A B C
Figure 8. Pressure gauge using
2. Install the PTFE filter on the rear panel of CO2-O2-UNIT-BL [0-10; 1-18]. It filters the mixed gas before it
enters in contact with CO2-O2-UNIT-BL [0-10; 1-18] gas sensors. Connect the PTFE filter with the Filter In
and Filter Out connectors on the rear panel of CO2-O2-UNIT-BL [0-10; 1-18] (see Figure 9 and pointer 1 and
2 in Figure 5).
Note ►CO2-O2-UNIT-BL [0-10; 1-18] cannot operate if the PTFE filter is not connected.
Figure 9. Filter tubing
Tip ► Replace this filter each 2-3 months of continuous use or when it is present water in the inner part of
the filter.
Do not remove the filters. This may cause damages the gas sensors.

15
3. Connect CO2 and N2 supply to the corresponding push to fit connectors on the rear panel of CO2-O2-UNIT-BL
[0-10; 1-18] by using TUBE-A (see Figure 10 a).
Tip ►Make sure to push the tubes all the way into the connectors thus avoiding any gas leak.
Note ►Do not exceed 1.5 barg (20 psig) for CO2, N2 and Air pressure.
To remove tubing from the connectors, push the red rubber ring while pulling the tubing (see Figure 10 b).
Tip ► If the tubing does not come out easily, do not force it, simply make sure the red ring is properly pushed.
a b
Figure 10. How to connect (a) and disconnect (b) tubings from push to fit connectors.
Before disconnecting any of tubing connected to CO2-O2-UNIT-BL [0-10; 1-18], make sure there is
no residual pressure by adjusting the pressure gauges upstream of the system and the tanks.
3. Figure 11 shows the connections between the CO2, N2and Air tanks and the respective connectors on the rear
panel of CO2-O2-UNIT-BL [0-10; 1-18] (see pointer 3 in Figure 1).

16
TUBE A
Not supplied
Air
A
CO2
A
N2
A
Figure 11. CO2, N2and Air Tanks connections to the back panel of the CO2/O2 Controller
Tip ►If you have ordered OKO-AIR-PUMP-BL, you have to connect TUBE-B between the Air Pump and the
connector labeled “Air” on the rear panel of CO2-O2-UNIT-BL [0-10; 1-18] (see Figure 12).

17
CO2
A
B
TUBE A
TUBE B
Tubing connection not supplied
A
N2
Figure 12. CO2, N2 Tanks and Air Pump connections to the back panel of the CO2/O2 Controller
4. Connect the TUBE-C to the gas port labeled “Gas output” on the rear panel of CO2-O2-UNIT-BL [0-10; 1-18]
and the other side of the TUBE-C to your device, as shown in Figure 13.

18
TUBE C
Gas Input
of your device
C
Figure 13. Gas Output connections.
Note ► If you purchased CO2-O2-UNIT-BL [0-10; 1-18] with H201-T-UNIT-BL or H101-T-UNIT-BL, use the
TUBE-Y supplied to connect CO2-O2-UNIT-BL [0-10; 1-18] gas output to the cage incubator (see Figure 14).
TUBE Y
Y
Figure 14. Gas Output connections with a Cage Incubator.
5. Connect the touch screen interface OKO-TOUCH to CO2-O2-UNIT-BL [0-10; 1-18] with the RS232 serial cable
as illustrated in Figure 15.

19
OKO-TOUCH
CO2-O2-UNIT-BL [0-10; 1-18]
Figure 15. Oko Touch Connection
6. Connect the power adapter to CO2-O2-UNIT-BL [0-10; 1-18] (see pointer 8 in Figure 1).
7. To switch CO2-O2-UNIT-BL [0-10; 1-18] on use the power button on OKO-TOUCH. The Status LED (1 in Figure
4) on the front of CO2-O2-UNIT-BL [0-10; 1-18] will turn green.
8. Once the system has been initialized the Status LED on the front of CO2-O2-UNIT-BL [0-10; 1-18] will stop
blinking and will turn steadily on. The Homepage will appear on the touch screen, the color of the Status
Indicator (see 1 in Figure 18 and Figure 23) on the gas tab will be yellow at the start.
9. Select the Setpoint gas composition from the touch screen Home page (see paragraph 9.1.1).
10. The light indicator on the Home page becomes green when the Setpoint compositions are reached and are
stable.
How to assemble CO2-O2-UNIT-BL [0-10; 1-18] with a Bold Line T controller
CO2-O2-UNIT-BL [0-10; 1-18] can be used as a stand-alone device or in combination with any one of the
Bold Line T controller.
Figure 16 shows how to assemble CO2-O2-UNIT-BL [0-10; 1-18], for example, with a H301-T UNIT-BL-
PLUS.

20
a
b.
Figure 16. CO2-O2-UNIT-BL [0-10; 1-18] and H301-T-UNIT-BL PLUS (a –b).
CO2-O2-UNIT-BL [0-10; 1-18] must stack on top of the Bold Line T controller by lining up the bus ports
located on the top and bottom surface of each unit. When the units are properly connected the Temperature and
CO2/O2parameters will appear on the OKO-TOUCH Touch Screen interface Home page.
Tip ► If not all the parameters appear on OKO-TOUCH Home Page then the Control Units are not properly
connected. Please check that the bus ports are properly aligned (see Figure 17).
Figure 17. CO2-O2-UNIT-BL [0-10; 1-18] and H301-T-UNIT-BL PLUS on stock.