Okolab CO2-O2-UNIT-BL User manual

CO2-O2-UNIT-BL [0-10; 0-1]
Gas Controller
Manual
IST2180_REV01
SV 2.2.5

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Index
1PREFACE .....................................................................................................................................................................................1
2SYMBOL DESCRIPTION ..........................................................................................................................................................2
SYMBOLS USED IN THIS MANUAL...............................................................................................................................................................2
SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................2
3SAFETY NOTES..........................................................................................................................................................................3
4SUPPLIED EQUIPMENT..........................................................................................................................................................5
5GAS SUPPLIED REQUIREMENTS .........................................................................................................................................6
6COMPATIBILITY.......................................................................................................................................................................6
7EQUIPMENT DESCRIPTION ..................................................................................................................................................8
8INSTALLATION ...................................................................................................................................................................... 10
SETTING UP CO2-O2-UNIT-BL [0-10; 0-1]....................................................................................................................................10
HOW TO ASSEMBLE CO2-O2-UNIT-BL [0-10; 0-1] WITH A BOLD LINE TCONTROLLER .......................................................18
9USER INTERFACE .................................................................................................................................................................. 20
HOME PAGE................................................................................................................................................................................................20
9.1.1 How to enter the Setpoint .......................................................................................................................................................20
9.1.2 Controller Status: colours led and meaning ....................................................................................................................22
SETTINGS....................................................................................................................................................................................................23
9.2.1 Gas......................................................................................................................................................................................................23
9.2.1.1 Air Source............................................................................................................................................................................................................23
9.2.1.2 Calibration..........................................................................................................................................................................................................25
9.2.1.2.1 Offset (with exthernal meter).............................................................................................................................................................25
9.2.1.2.2 Sensors (with calibration gas)........................................................................................................................................................... 27
9.2.1.2.2.1 View ......................................................................................................................................................................................................28
9.2.1.2.2.2 Span Settings .................................................................................................................................................................................... 28
9.2.1.2.2.3 Factory Reset ....................................................................................................................................................................................29
9.2.1.2.2.4 Start Span........................................................................................................................................................................................... 29
9.2.1.2.2.5 Start Zero............................................................................................................................................................................................30
9.2.1.2.3 Factory Reset.............................................................................................................................................................................................31
9.2.1.3 Flowrates............................................................................................................................................................................................................. 31
9.2.1.4 Gas cycles............................................................................................................................................................................................................. 33
9.2.1.5 Status ....................................................................................................................................................................................................................36
9.2.2 Touch Screen configuration ...................................................................................................................................................37
9.2.2.1 Touch Screen Options.....................................................................................................................................................................................37
9.2.2.2 Touch Screen Brightness ..............................................................................................................................................................................37
9.2.2.3 Touch Screen Visual Effects –icon and glance mode.......................................................................................................................38
9.2.2.4 Date & Time........................................................................................................................................................................................................ 38
9.2.2.5 Alarms...................................................................................................................................................................................................................39
9.2.2.6 Data Logging..................................................................................................................................................................................................... 40
OVERVIEW..................................................................................................................................................................................................44
ICON AND GLANCE MODE VIEW.............................................................................................................................................................44
INFO PAGE ..................................................................................................................................................................................................45
10 TOUCH SCREEN CALIBRATION .................................................................................................................................... 46
11 CLEANING & MAINTENANCE......................................................................................................................................... 47
12 APPENDIX: OXYGEN ANALYZER .................................................................................................................................. 48
MAINTENANCE..........................................................................................................................................................................................48
PERIODIC CALIBRATION ..........................................................................................................................................................................48
O2 SENSOR REPLACEMENT -CAUTIONS...............................................................................................................................................48
O2 SENSOR REPLACEMENT -PROCEDURE...........................................................................................................................................49
13 SUPPORT ............................................................................................................................................................................. 52
14 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 53
15 TROUBLESHOOTING........................................................................................................................................................ 54

16 FIGURE LIST........................................................................................................................................................................ 55
17 MANUAL REVISION TABLE ............................................................................................................................................ 56

1
1Preface
CO2-O2-UNIT-BL [0-10; 0-1] is a Digital CO2/O2 Controller that mixes CO2, N2 and Air to the desired
concentration in the CO2 range 0-10% and O2 range 0-1%.
It features a long life Non Dispersive Infrared (NDIR) dual wave length CO2 detector and a O2
electrochemical sensor to ensure a high accuracy on the gas composition and a flow rate sensor to regulate output
flow rate.
CO2-O2-UNIT-BL [0-10; 0-1] is operated via the OKO-TOUCH touch screen control panel (to be ordered
separately). OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition, data logging is possible via SMART-BOX, via DATA-LOG Okolab software and via following
third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN
Black software is available upon request.
Okolab Bold Line controllers are also capable to receive TTL input (requires to order separately TTL-
CABLE).
CO2-O2-UNIT-BL [0-10; 0-1] connects to any Okolab Bold Line T controller. In this case, a single OKO-
TOUCH operates both, the Bold Line T controller and CO2-O2-UNIT-BL [0-10; 0-1].
CO2-O2-UNIT-BL [0-10; 0-1] is also compatible with Okolab Active Humidity Controller to control output
gas Relative Humidity and with Okolab air pump OKO-PUMP-BL, which enables to use background air as a
convenient alternative to the compressed air.

2
2Symbol description
This paragraph describes the symbols used in this manual and on the product label.
Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully use the instrument.
Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.
This symbol indicates the product production date.
This symbol indicates the Manufacturer data.
This symbol warns you to read the user manual before starting the device.
This symbol indicates the protection degree against ingress of solids or liquids inside the
product.
IP 30

3
3Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.
−The equipment must only be used as intended and as described in this Manual.
−Equipment should be operated only by technically qualified personnel.
−Do not start up the equipment if some of its parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Transport the equipment with care.
−Equipment and its internal parts can be damaged by dropping and by shock.
−Not following these instructions can result in damage or breakdown of the device and its
accessories.
−The products labels can be found on the bottom panel of the Main Box.
−Do not disassemble any part of the system.
−Do not use a volatile solvent such as paint thinner to clean the instrument, because
deformation or discoloration may occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Do not exceed voltage indicated in this manual and on the product label.
−Avoid excessive induction noise, static electricity and magnetic fields.
−Do not expose this instrument to rain or moisture.
−Do NOT go in close contact with or breathe any gas stream whose composition is different
from that of ambient air.
−Prevent throttling and kinking of tubing.
−Check tubing time to time for possible material usage.
−Check that all tubing ends are well inserted into the connectors so they cannot slip off
−This device is not designed for use for medical applications.
−Install safety valves and adequate pressure regulators on gas lines before CO2-O2-UNIT-
BL [0-10; 0-1] input connectors.
−Power cord of unit should be unplugged from electrical outlet when left unused for a long
period of time.
−PRESSURIZED GAS. Secure all connections with hose clamps. Never exceed the input
pressure limit of 5 barg (72.5 psig). Bleed all lines before disconnecting. Wear safety
goggles if needed. If pressure regulators are not within sight and reach, make sure at least
one shut off valve is within reach.
−LOW OXYGEN ATMOSPHERES. Never enter a room or enclosure which has a low oxygen
atmosphere because of severe danger of suffocation. Only operate in well-ventilated room.
A small amount for carbon dioxide gas leaks continuously out of the instrument and should
never be allowed to build up in the room.
−VENTILATION. Unit should be situated so that its location or position does not interfere
with proper ventilation. Neither the gas mixer nor stream destinations should be in poorly
ventilated areas.
−Unit should be situated away from heat sources such as open flames, radiators, heat
registers, stoves, or other appliances or processes that produce heat.

4
−Do not start up the equipment if the supply cable is damaged.
−Connect the equipment only to grounded mains power socket.
−Do not disconnect cables while in operation.
−Do not open the unit. Do not remove cover or back.
−Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric
shock, fire or malfunction.
−No user serviceable parts inside.
−Unit should never be used where it can fall or be pushed into water.
International caution symbol marks this device. It is important to read the “Safety
Notes” before installing, using and commissioning this device, as the notes contain
important information relating to safety and EMC. Not following these instructions
can result in damage or breakdown of the device and its accessories
We reserve the right to make technical variations.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE
OF ANY PRODUCT.

5
4Supplied equipment
1. CO2-O2-UNIT-BL [0-10; 0-1], main box.
2. TUBE-CB (x2). Silicon tube 20cm long 7mm OD with a PTFE filter in the middle. One to be installed on the
rear of the Unit and one as spare replacement.
3. TUBE-C (x1). Polyurethane 130cm long blue rigid tube 6mm OD with connected a 4mm ID Silicon
transparent tube 150 cm long plus PTFE filter in the middle. Use TUBE C to connect the CO2/O2 Controller
to your device or to the humidifier, if it is present.
Note ►If you purchased CO2-O2-UNIT-BL [0-10; 0-1] with H201-T-UNIT or H101-T-UNIT, you receive the
TUBE-B, Polyurethane 120 cm long blue tube 6mm OD connected to a Silicon 40 cm long transparent tube
4mm ID plus PTFE filter in the middle, connected to a Polyurethane 120 cm long blue tube 6mm OD. Use
TUBE-B to connect the CO2-O2-UNIT-BL [0-10; 0-1] to the cage incubator.
4. TUBE-A (x3). Rigid tubes, Polyurethane 3m long 6mm OD each. Use TUBE-A to connect N2, Air and CO2 supply
to the corresponding input ports (N2, Air and CO2) on the rear panel of the CO2-O2-UNIT-BL [0-10; 0-1].
5. Pressure Gauge for CO2/N2 + regulator + assembly stirrup (x2). Install pressure gauge between the pure
CO2 /N2 supply pressure regulator and the corresponding CO2/N2 input port on the rear panel of CO2-O2-
UNIT-BL [0-10; 0-1].
6. 24V-DC Power Adapter (x1).
7. Oxygen Analyzer Tube (x2). PTFE tube 135 mm long 4mm OD. Use Oxygen Analyzer Tube to connet the
Oxygen Analyzer to the main box.
1
4
5
3
2
To N2 supply
To CO2 supply
6
To Air supply
To N2 supply
5
7
Figure 1. CO2-O2-UNIT-BL [0-10; 0-1] –components.

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5GAS supplied requirements
−CO2. CO2 must be available at 1.4 barg (20.3 psig), with a connector for 6mm OD rigid tube. Gas source
must be Standard Purity CO2 (coded as 4.5 that means 99.995 % of CO2) and humidity-free gas.
−N2. N2 must be available within the pressure range at 1.4 barg (20.3 psig), with a connector for 6mm OD
rigid tube. Gas source must be Standard Purity N2 (coded as 4.5 that means 99.995 % of N2) and humidity-
free gas.
−Air. Air must be available at 1.4 barg (20.3 psig), with a connector for 6mm OD rigid tube.
Note ►An alternative to the compressed air, OKO-AIR-PUMP-BL allows to use background Air.
6Compatibility
CO2-O2-UNIT-BL [0-10; 0-1] is compatible with:
•OKO-TOUCH (required), including:
−Touch Screen Interface
−#1 RS232 Serial cable. To connect the Touch Screen to the unit.
−MINI-USB-OTG cable. To connect a USB pen to the OKO-TOUCH for data download.
•OKO-AIR-PUMP-BL (only in case it is required to work in “CO2 in Air” mode)
•H101-BASIC-BL
•H101-CRYO-BL
•H201-T-UNIT-BL
•H301-T-UNIT-BL-PLUS
•HM-ACTIVE
•H101-HM
•HM-VF
OKO-AIR-PUMP-BL
HM-ACTIVE
H301-T-UNIT-BL
OKO-TOUCH
Figure 2. CO2-O2-UNIT BL [0-10; 0-1] within a typical H301 configuration.

7
•DATA-LOG. Software used to log, view, and store data from a PC. It may be used to change parameters of
the controllers via PC. Data can be exported in .xlsx format.
Figure 3. DATA LOG software.
•SM-BL Smart Box. To store data, to operate the controllers via web/smartphone/tablet and to allow
remote assistance for service.

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7Equipment Description
Figure 4 illustrates CO2-O2-UNIT-BL [0-10; 0-1] front panel:
1. Status Led.
2. RS232 connector. Connector for OKO-TOUCH.
1 2
Figure 4. Gas Controller Front Panel Overview.
Figure 5 illustrates CO2-O2-UNIT-BL [0-10; 0-1] rear panel:
1. Filter Out. Connector for TUBE-CB.
2. Filter In. Connector for TUBE-CB.
3. Gas Output. Push to fit Input connector for 6 mm O.D. rigid tubing to supply mixed gas to the user system.
4. Power Input.
5. OKO-AIR-PUMP-BL connector. Leave unplugged if CO2-O2-UNIT-BL [0-10; 0-1] is not equipped with OKO-
AIR-PUMP-BL.
6. HM-ACTIVE connector. Leave unplugged if CO2-O2-UNIT-BL [0-10; 0-1] is not equipped with HM-ACTIVE.
7. GPIO (General Purpose Input Output) connector.
8. USB connector.
9. N2 Input. Push to fit Input connector for 6 mm O.D. rigid tubing to supply N2 to the mixer.
10. Air Input. Push to fit Input connector for 6 mm O.D. rigid tubing to supply Air to the mixer.
11. CO2 Input. Push to fit Input connector for 6 mm O.D. rigid tubing to supply CO2 to the mixer.

9
1 2 3
4 5 6 7 8 911
10
Figure 5. Gas Controller Rear Panel Overview.
Figure 6 illustrates CO2-O2-UNIT-BL [0-10; 0-1] left panel:
1. Oxygen Analyzer includes the O2 sensor.
2. Oxygen Analyzer –Gas input connector. Push to fit connector (4 mm OD rigid tube) for mixed gas input to
O2 Sensor.
3. Oxygen Analyzer–Gas output connector. Push to fit connector (4 mm OD rigid tube) for mixed gas output
from O2 Sensor.
1
2
3
Figure 6. Gas Controller –Left Panel Overview

10
8Installation
The following paragraph illustrates how to install and to use CO2-O2-UNIT-BL [0-10; 0-1].
Setting up CO2-O2-UNIT-BL [0-10; 0-1]
The following instructions shall give you the possibility to install the unit quickly. For safe operation
of the unit it is absolutely necessary to read carefully all the instructions and safety notes.
1. Install the supplied pressure gauge between the CO2 supply and CO2-O2-UNIT-BL [0-10; 0-1]. Install the
supplied pressure gauge between the N2 supply and CO2-O2-UNIT-BL [0-10; 0-1]. Follow the arrow on the
rear of the pressure gauge for the correct Gas In-Out (see Figure 7).
GAS IN GAS OUT
Figure 7. Input and Output gas ports. See symbol on the rear of pressure gauge.
On the bottom of the pressure gauge there is an automatic purging valve. Close it before operating the
pressure gauge. To close the purging valve, looking at the regulator vertically from the top knob, pull it down and
rotate it counterclockwise (see A and B in Figure 8). Now you can operate the pressure gauge.
Purging valve
A B
Figure 8. How to close the purging valve of the pressure gauge.

11
To close the gauge, pull the knob up and rotate it counterclockwise. Push the knob down to lock it. Open
valve on the gas supply tank. Adjust Pressure Gauge by pulling the top knob up and rotating it clockwise until the
pressure reaches the desired value (see Figure 9), then push the top knob down to lock it.
A B C
Figure 9. Pressure gauge using.
2. Install the TUBE-CB on the rear panel of CO2-O2-UNIT-BL [0-10; 0-1]. It filters the mixed gas before it enters
in contact with CO2-O2-UNIT-BL [0-10; 0-1] gas sensors. Connect the TUBE-CB with the Filter In and Filter Out
connectors on the rear panel of CO2-O2-UNIT-BL [0-10; 0-1] (1, 2 in Figure 5 and Figure 10). Make sure the
Filter In label side of the filter is always connected to Filter In connector on the rear panel of CO2-O2-UNIT-BL
[0-10; 0-1].
Note ►CO2-O2-UNIT-BL [0-10; 0-1] cannot operate if the TUBE-CB is not connected.
Note ►The improper installation of the TUBE-CB may cause gas sensors contamination.
Figure 10. How to install TUBE-CB.
Tip ► Replace this filter each 2-3 months of continuous operation or if you see water in the inner part of the
filter.

12
Do not remove the filter. This may damage the gas sensors.
3. Connect N2, Air and CO2 supply to the corresponding push to fit connectors on the rear panel of CO2-O2-UNIT-
BL [0-10; 0-1] by using TUBE-A (see Figure 11 a). Set N2, Air and CO2 pressure at 1.4 barg (20.3 psig).
Tip ►Make sure to push the tubes all the way into the connectors thus avoiding any gas leak.
Note ►Do not exceed 5 barg (72.5 psig) for CO2 and N2 supply.
To remove tubing from the connectors, push the red rubber ring while pulling the tubing (see Figure 11 b).
Tip ► If the tubing does not come out easily, do not force it, simply make sure the red ring is properly pushed.
ab
Figure 11. How to connect (a) and disconnect (b) tubings from push to fit connectors.
Before disconnecting any of tubing connected to CO2-O2-UNIT-BL [0-10; 0-1], make sure there is
no residual pressure by adjusting the pressure gauges upstream of the system and the tanks.
.
4. To install the O2 Sensor follow the instructions below:
•Carefully open the bag of the O2 Sensor using a pair of scissors or a knife. Make sure that there is no
sign of any liquid in the bag, if so, do not proceed, you need a new sensor. Be careful that you don’t
poke anything such as a fingernail through the membrane.
•Remove the plug or other device that acts as a shorting clip. This may be found on the connection
plate on the back of the O2 Sensor.
•Slide the O2-Sensor into the sensor unit (gold plated contact side of sensor should be facing up
touching the sensor unit contacts. The membrane side is covered by a convex gold-plated mesh). Be
careful not to touch the membrane while doing this - if the membrane is punctured the O2 Sensor
must be replaced (see image 1 in Figure 12)
•Verify that the sealing O ring is in place in the cell cap groove (see image 2 in Figure 12)

13
•Carefully place the cap and turn it clockwise. Make sure that you do not cross thread it, and tighten
firmly by hand. Do not over-tighten (see image 4 in Figure 12).
•Connect the Oxygen Analyzer tubes are connected to the Oxygen Analyzer –Gas input connector and
Oxygen Analyzer –Gas output connector (see image 5 in Figure 12). Make sure that the Oxygen
analyzer tubes are securely connected.
Tip ►Make sure to push the tubes all the way into the connectors thus avoiding any gas leak.
12
34
5 6
O2 SENSOR
ORING
Figure 12. O2 Sensor installation.
5. Figure 13 shows the connections between the N2, Air and CO2 tanks and the respective connectors on the rear
panel of CO2-O2-UNIT-BL [0-10; 0-1].

14
TUBE-A
NOT supplied
N2 CO2
A
A
Air
A
Figure 13. N2, Air and CO2 tanks connections to the back panel of CO2-O2-UNIT-BL [0-10; 0-1].
Tip ►If you have ordered OKO-AIR-PUMP-BL, connect TUBE-B between the OKO-AIR-PUMP-BL and the
connector labeled Air on the rear panel of CO2-O2-UNIT-BL [0-10; 0-1] (see Figure 14).

15
TUBE-A
TUBE-B
NOT supplied
N2 CO2
A
B
A
Figure 14. N2, CO2 and Air Pump connections to the rear panel of CO2-O2-UNIT-BL [0-10; 0-1].
6. Connect one end of the TUBE-C to the push to fit connector of the port labeled “Gas output” on the rear panel
of CO2-O2-UNIT-BL [0-10; 0-1] and the other side of the TUBE-C to your device, as shown in Figure 15.
TUBE-C
Gas Input
of your device
C
Figure 15. Gas Output connections.

16
Note ► If you purchased CO2-O2-UNIT-BL [0-10; 0-1] with H201-T-UNIT-BL or H101-T-UNIT-BL, use the
TUBE-B supplied to connect the CO2-O2-UNIT-BL [0-10; 0-1] gas output to the cage incubator (see Figure
16).
TUBE-B
B
Figure 16. Gas Output connections with a Cage Incubator.
Note ► If you purchased CO2-O2-UNIT-BL [0-10; 0-1] with H301-T-UNIT-BL-PLUS and HM-ACTIVE use the
supplied TUBE-C to connect the CO2-O2-UNIT-BL [0-10; 0-1] gas output to the to the port labeled “Gas Input”
on the Humidity Sensor Lid of HM-ACTIVE (see Figure 17). Connect the Heated Based cable of HM-ACTIVE to
the port labeled “H.M.” on the rear panel of CO2-O2-UNIT-BL [0-10; 0-1] (see Figure 17).
HM-ACTIVE CO2-O2-UNIT-BL [0-10; 0-1]
TUBE C
Figure 17. Gas Output connections with HM-ACTIVE.
Note ► If you purchased CO2-O2-UNIT-BL [0-10; 0-1] with H101-T-UNIT-BL- use the supplied TUBE-C to
connect the CO2-O2-UNIT-BL [0-10; 0-1] gas output to the push in fitting connection on the cover of the
thermostat (see Figure 18).