Okolab CO2-UNIT-BL User manual

CO2-UNIT-BL
Gas Controller
Manual
IST1140_REV03
SV 3.12.3.3048

This page was left Blank

Index
1PREFACE .....................................................................................................................................................................................1
2SYMBOL DESCRIPTION ..........................................................................................................................................................2
SYMBOLS USED IN THIS MANUAL...............................................................................................................................................................2
SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................2
3SAFETY NOTES..........................................................................................................................................................................3
4SUPPLIED EQUIPMENT..........................................................................................................................................................5
5GAS SUPPLIED REQUIREMENTS .........................................................................................................................................5
6COMPATIBILITY.......................................................................................................................................................................6
7EQUIPMENT DESCRIPTION ..................................................................................................................................................8
8INSTALLATION ...................................................................................................................................................................... 10
SETTING UP CO2-UNIT-BL...................................................................................................................................................................10
HOW TO ASSEMBLE CO2-UNIT-BL WITH A BOLD LINE TCONTROLLER......................................................................................14
9USER INTERFACE .................................................................................................................................................................. 16
HOMEPAGE.................................................................................................................................................................................................16
9.1.1 How to enter the Setpoint .......................................................................................................................................................16
9.1.2 Controller Status: colors led and meaning.......................................................................................................................18
SETTINGS....................................................................................................................................................................................................19
9.2.1 Gas......................................................................................................................................................................................................19
9.2.1.1 Air Source............................................................................................................................................................................................................19
9.2.1.2 Calibrations........................................................................................................................................................................................................ 21
9.2.1.2.1 Offset (with exthernal meter).............................................................................................................................................................21
9.2.1.2.2 Sensors (with calibration gas)........................................................................................................................................................... 23
9.2.1.2.2.1 View ......................................................................................................................................................................................................24
9.2.1.2.2.2 Span Settings .................................................................................................................................................................................... 24
9.2.1.2.2.3 Factory Reset ....................................................................................................................................................................................24
9.2.1.2.2.4 Start Span........................................................................................................................................................................................... 25
9.2.1.2.2.5 Start Zero............................................................................................................................................................................................25
9.2.1.2.3 Factory Reset.............................................................................................................................................................................................26
9.2.1.3 Flowrates............................................................................................................................................................................................................. 27
9.2.1.4 Gas cycles............................................................................................................................................................................................................. 28
9.2.1.5 Status ....................................................................................................................................................................................................................30
9.2.1.6 Digital Input.......................................................................................................................................................................................................30
9.2.2 Touch Screen configuration ...................................................................................................................................................31
9.2.2.1 Touch Screen Options..................................................................................................................................................................................... 31
9.2.2.2 Touch Screen Brightness ..............................................................................................................................................................................32
9.2.2.3 Touch Screen Visual Effects –icon and glance mode.......................................................................................................................32
9.2.2.4 Date & Time........................................................................................................................................................................................................ 33
9.2.2.5 Alarms...................................................................................................................................................................................................................33
9.2.2.6 Data Logging .....................................................................................................................................................................................................34
OVERVIEW..................................................................................................................................................................................................39
ICON AND GLANCE MODE VIEW.............................................................................................................................................................39
INFO PAGE ..................................................................................................................................................................................................39
10 TOUCH SCREEN CALIBRATION .................................................................................................................................... 41
11 CLEANING & MAINTENANCE......................................................................................................................................... 42
12 SUPPORT ............................................................................................................................................................................. 43
13 TECHNICAL SPECIFICATIONS ....................................................................................................................................... 44
14 TROUBLESHOOTING........................................................................................................................................................ 45
15 FIGURE LIST........................................................................................................................................................................ 46
16 MANUAL REVISION TABLE ............................................................................................................................................ 47

1
1Preface
CO2-UNIT-BL is a Digital CO2 Controller that mixes CO2 and Air to the desired concentration in the range
0-18%. It connects to any Bold Line T controller and can be operated through the same intuitive touch screen
interface, OKO-TOUCH.
The CO2-UNIT-BL features a long life Non Dispersive InfraRed (NDIR) dual wave length detector to
ensure a high accuracy on CO2 concentration and a flow rate sensor to regulate output flow rate.
The CO2-UNIT-BL is compatible with Okolab Active Humidity Controller to control output gas Relative
Humidity and with Okolab air pump OKO-PUMP-BL, which enables to use background air as a convenient
alternative to compressed air.
The CO2-UNIT-BL is operated via the OKO-TOUCH touch screen control panel (to be ordered separately).
OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition data logging is possible via SMART-BOX, via DATA-LOG Okolab software and via following
third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN
Black software is available upon request.
Okolab Bold Line controllers are also capable to receive TTL input (requires to order separately TTL-
CABLE).

2
2Symbol description
This paragraph describes the symbols used in this manual and on the product label.
Symbols used in this manual
The following symbols identify important information to note:
CAUTION or WARNING: this symbol warns you about the risk of electrical shock.
CAUTION or WARNING or IMPORTANT: this symbol warns you of circumstances or practices that
can affect the functionality of the instrument.
Tip ►Supplies you with helpful suggestions.
Note ►Supplies you with important information to successfully use the instrument.
Symbols on the product label
CE MARKING: this symbol indicates product compliance with EU legislation.
PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid
waste.
This symbol indicates the product production date.
This symbol indicates the Manufacturer data.
This symbol warns you to read the user manual before starting the device.
This symbol indicates the protection degree against ingress of solids or liquids inside the product.
IP 30

3
3Safety Notes
In order to achieve maximum performance and to ensure proper operation of your new equipment, please
read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.
−The equipment must only be used as intended and as described in this Manual.
−Equipment should be operated only by technically qualified personnel.
−Do not start up the equipment if some of its parts are damaged.
−This instrument is not intended for use in locations subject to flammable or explosive gases.
−Transport the equipment with care.
−Equipment and its internal parts can be damaged by dropping and by shock.
−Not following these instructions can result in damage or breakdown of the device and its
accessories.
−The products labels can be found on the bottom panel of the Main Box.
−Do not disassemble any part of the system.
−Do not disconnect cables while in operation.
−Do not use a volatile solvent such as paint thinner to clean the instrument, because
deformation or discoloration may occur.
−Use a soft, dry cloth to remove stains from the instrument.
−Do not exceed voltage indicated in this manual and on the product label.
−Avoid excessive induction noise, static electricity and magnetic fields.
−Do not expose this instrument to rain or moisture.
−Do NOT go in close contact with or breathe any gas stream whose composition is different
from that of ambient air.
−Prevent throttling and kinking of tubing.
−Check tubing time to time for possible material usage.
−Check that all tubing ends are well inserted into the connectors so they cannot slip off.
−This device is not designed for use for medical applications.
−Install safety valves and adequate pressure regulators on gas lines before CO2 Controller
input connectors.
−Power cord of unit should be unplugged from electrical outlet when left unused for a long
period of time.
−PRESSURIZED GAS. Secure all connections with hose clamps. Never exceed the input
pressure limit of 2.5 barg (36.3 psig). Bleed all lines before disconnecting. Wear safety
goggles if needed. If pressure regulators are not within sight and reach, make sure at least
one shut off valve is within reach.
−LOW OXYGEN ATMOSPHERES. Never enter a room or enclosure which has a low oxygen
atmosphere because of severe danger of suffocation. Only operate in well-ventilated room.
A small amount for carbon dioxide gas leaks continuously out of the instrument and should
never be allowed to build up in the room.
−VENTILATION. Unit should be situated so that its location or position does not interfere
with proper ventilation. Neither the gas controller nor stream destinations should be in
poorly ventilated areas.
−Unit should be situated away from heat sources such as open flames, radiators, heat
registers, stoves, or other appliances or processes that produce heat.

4
−Do not start up the equipment if the supply cable is damaged.
−Connect the equipment only to grounded mains power socket.
−Do not disconnect cables while in operation.
−Do not open the unit. Do not remove cover or back.
−Prevent metal fragments or lead wire scraps from falling inside instrument to avoid electric
shock, fire or malfunction.
−No user serviceable parts inside.
−Before starting, assemble the equipment far from electric device.
−Unit should never be used where it can fall or be pushed into water.
International caution symbol marks this device. It is important to read the “Safety
Notes” before installing, using and commissioning this device, as the notes contain
important information relating to safety and EMC. Not following these instructions
can result in damage or breakdown of the device and its accessories.
We reserve the right to make technical variations.
IN NO EVENT SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL OR CONSEQUENTIAL
DAMAGES OF ANY NATURE, OR LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT OR THE USE
OF ANY PRODUCT.

5
4Supplied equipment
1. CO2 Controller, main box.
2. TUBE-CB (x2). Silicon tube 20cm long 7mm OD with a PTFE filter in the middle. One to be installed on the
rear of the Unit and one spare replacement.
3. TUBE-A (x2). Rigid tubes, 3m long 6mm OD each. Use TUBE A to connect the output ports of Air and Carbon
Dioxide tanks to the corresponding input ports (Air and CO2) on the rear panel of the CO2 Controller.
4. TUBE-C (x1). 2m long blue rigid tube 6mm OD with attached a 4mm ID silicon transparent tube 1 m long plus
PTFE filter in the middle. Use TUBE C to connect the CO2 Controller to your device or to the humidifier, if it is
present.
5. Pressure Gauge for CO2 + regulator + assembly stirrup (x1). Install pressure gauge between the pure CO2
tank pressure regulator and the CO2 Controller input port.
6. 24V-DC Power Adapter (x1).
Figure 1. CO2-UNIT-BL –components.
5GAS supplied requirements
−CO2. CO2 must be available at 1.4 barg (20.3 psig), with a connector for 6mm OD rigid silicon tube. Gas
source must be Standard Purity CO2 (coded as 4.5 that means 99.995 % of CO2) and humidity-free gas.
−Air. Air must be available at 1.4 barg (20.3 psig), with a connector for 6mm OD rigid silicon tube.
Note ►An alternative solution for Air supply is the OKO-AIR-PUMP-BL.

6
6Compatibility
CO2-UNIT-BL is compatible with:
•OKO-TOUCH (required), including:
−Touch Screen Interface
−#1 RS232 Serial cable. To connect the Touch Screen to the unit.
−MINI-USB-OTG cable. To connect a USB pen to the OKO-TOUCH for data download.
•OKO-AIR-PUMP-BL
•H101-BASIC-BL
•H101-CRYO-BL
•H201-T-UNIT-BL
•H301-T-UNIT-BL-PLUS
•HM-ACTIVE
•H101-HM
•HM-VF
Figure 2. CO2-UNIT BL within a typical H301 configuration.

7
•DATA-LOG (Optional-to be purchased separately). Software used to log, view, and store data from a PC.
It may be used to change parameters of the controllers via PC. Data can be exported in .xlsx format.
Figure 3. DATA LOG software.
•SM-BL Smart Box. To store data, to operate the controllers via web/smartphone/tablet and to allow
remote assistance for service.

8
7Equipment Description
Figure 4 illustrates CO2-UNIT-BL front panel:
1. Status Led.
2. RS232 connector. Connector for OKO-TOUCH.
Figure 4. Gas Controller Front Panel Overview.
Figure 5 illustrated CO2-UNIT-BL rear panel:
3. Filter Out. Connector for the TUBE-CB.
4. Filter In. Connector for the TUBE-CB.
5. Gas Output.
6. Power Input.
7. OKO-AIR-PUMP-BL connector. Leave unplugged if CO2-UNIT-BL is not equipped with OKO-AIR-PUMP-BL.
8. HM-ACTIVE connector. Leave unplugged if CO2-UNIT-BL is not equipped with HM-ACTIVE.
9. GPIO (General Purpose Input Output) connector.
10. USB connector.
11. Air Input. Push to fit Input connector for 6 mm O.D. rigid tubing to supply air to the unit.
12. CO2 Input. Push to fit Input connector for 6 mm O.D. rigid tubing to supply CO2 to the unit.

9
Figure 5. Gas Controller Rear Panel Overview.

10
8Installation
The following paragraph illustrates how to install and to use CO2-UNIT-BL.
Setting up CO2-UNIT-BL
The following instructions shall give you the possibility to install the unit quickly. For safe operation
of the unit it is absolutely necessary to read carefully all the instructions and safety notes.
1. Install the pressure Gauge for the CO2 line between the CO2 tank and CO2-UNIT-BL. Follow the arrow on
the rear of the Pressure Gauge for the correct Gas In-Out (see Figure 6).
GAS IN GAS OUT
Figure 6. Input and Output gas ports. See symbol on the rear of Pressure Gauge.
On the bottom of the Pressure Gauge there is an automatic purging valve. Close it before operating the
Pressure Gauge. To close the purging valve, looking at the regulator vertically from the top knob, pull it down and
rotate it counterclockwise (see A and B in Figure 7). Now you can operate the Pressure Gauge.
Purging valve
A B
Figure 7. How to close the purging valve of the Pressure Gauge.

11
To close the gauge, pull the knob up and rotate it counterclockwise. Push the knob down to lock it. Open
valve on the gas supply tank. Adjust Pressure Gauge by pulling the top knob up and rotating it clockwise until the
pressure reaches the desired value (see Figure 8), then push the top knob down to lock it.
A B C
Figure 8. Pressure gauge using.
2. Install the TUBE-CB on the rear panel of CO2-UNIT-BL. It filters the Air/CO2 flow before it enters in contact
with CO2-UNIT-BL gas sensors. Connect the TUBE-CB with the Filter In and Filter Out connectors on the rear
panel of CO2-UNIT-BL (3, 4 in Figure 5). Make sure the Filter In label side of the filter is always connected to
Filter In connector on the rear panel of CO2-UNIT-BL (see Figure 9).
Note ►CO2-UNIT-BL cannot operate if the TUBE-CB is not connected.
Note ►The improper installation of the TUBE-CB may cause gas sensors contamination.
Figure 9. How to install TUBE-CB.
Tip ►Replace this filter each 2-3 months of continuous operation or if you see water in the inner part of the
filter.

12
Do not remove the filter. This may damage the gas sensors.
3. Connect CO2 and air supply to the corresponding push to fit connectors on the rear panel of CO2-UNIT-BL by
using TUBE-A (see Figure 10 ).
Tip ►Make sure to push the tubes all the way into the connectors thus avoiding any gas leak.
Note ►Do not 2.5 barg (36.3 psig) for CO2 and Air supply.
To remove tubing from the connectors, push the red or black rubber ring while pulling the tubing.
Tip ► If the tubing does not come out easily, do not force it, simply make sure the rubber ring is properly
pushed.
Figure 10. How to connect tubing to push to fit connector.
Before disconnecting any of tubing connected to CO2-UNIT-BL, make sure there is no residual
pressure by adjusting the pressure gauges upstream of the system and the tanks.
4. Figure 11 shows the connections between the CO2and Air tanks and the respective connectors on the rear
panel of CO2-UNIT-BL (see pointer 3 in Figure 1).

13
Figure 11. CO2 and Air Tanks connections to the rear panel of the CO2 Controller.
Tip ►If you have ordered OKO-AIR-PUMP-BL, you have to connect TUBE B between the Air Pump and the
connector labeled “Air” on the rear panel of the CO2 Controller (see Figure 12).
Figure 12. CO2 Tank and Air Pump connections to the back panel of the CO2 Controller.
5. Connect the touch screen interface OKO-TOUCH to the CO2-UNIT-BL with the RS232 serial cable as illustrated
in Figure 13.

14
Figure 13. Oko Touch Connection.
6. Connect the power adapter to CO2-UNIT-BL (6 in Figure 1).
7. Regulate the pressure of each gas before the CO2 Controller at 1.4 barg (20.3 psig). Do not exceed 2.5 barg!
8. Check that all the cables are properly installed as described in the present paragraph.
9. To switch CO2-UNIT-BL on use the power button on OKO-TOUCH. The Status LED (1 in Figure 4) on the front
of CO2-UNIT-BL will turn green.
10. Once the system has been initialized the Status LED on the front of CO2-UNIT-BL will stop blinking and will
turn steadily on. The Homepage will appear on the touch screen, the color of the Status Indicator (see 1 in
Figure 16 and Figure 18) on the gas tab will be yellow at the start.
11. Select the Setpoint gas composition from the touch screen Homepage (see Paragraph 9.1.1).
12. The light indicator on the Homepage becomes green when the Setpoint compositions are reached and are
stable.
How to assemble CO2-UNIT-BL with a Bold Line T controller
CO2-UNIT-BL can be used as a stand-alone device or in combination with any one of the Bold Line
Microscope Incubator.
Figure 14 shows how to assemble CO2-UNIT-BL, for example, with a H301-T UNIT-BL-PLUS.

15
Figure 14. CO2-UNIT-BL and H301-T-UNIT-BL-PLUS.
CO2-UNIT-BL must stack on top of the Bold Line T controller by lining up the bus ports located on the top
and bottom surface of each unit. When the units are properly connected the Temperature and CO2 parameters
will appear on the OKO-TOUCH Touch Screen interface Homepage.
Tip ►If not all the parameters appear on OKO-TOUCH Homepage then the Control Units are not properly
connected. Please check that the bus ports are properly aligned (see Figure 15).
Figure 15. CO2-UNIT-BL and H301-T-UNIT-BL PLUS on stock.
If you have CO2-UNIT-BL and a Bold Line T controller, it is essential to read both this manual
and the Bold Line T controller User Manual to familiarize yourself with the functions and
the operation of the devices before use.

16
9User Interface
This chapter describes the user interface of CO2-UNIT-BL.
Homepage
Figure 16. Homepage of CO2-UNIT-BL Touch Screen Display.
1. Status Indicator.
2. CO2Setpoint (see 9.1.1 to learn how to change the Setpoint value).
3. CO2current value.
4. Min/max CO2values within the time-frame set in the Options subpage (see paragraph 9.2.2.1).
5. Chart history length set at 5 minutes.
6. Display mode. Touch here to switch display mode.
7. Overview page.
8. Settings. Press here to access system options and settings.
9. Home. To open the homepage.
10. Product info. Press here to know generic info about CO2-UNIT-BL and running time.
Tip ►The OKO-TOUCH is pre-set at a 5% CO2 concentration hence when you switch it on the system will
start operating to reach this set-point value.
9.1.1 How to enter the Setpoint
To input a new CO2 Setpoint, touch the corresponding tab, as indicated in Figure 17 a.

17
Figure 17. How to change the CO2Setpoint (a –b).
The Setpoint regulation page will appear as in the Figure 17 b. You can modify the Setpoint by clicking on
+ and –. Once you have input the new CO2Setpoint, press Set (2 in Figure 17 b) to save or Cancel to undo.
Tip ►After any change in the Setpoint value, CO2-UNIT-BL enters into a transient regime, the Status
Indicator and the TOP LED turn yellow (see paragraph 9.1.2). During the transient regime, CO2-UNIT-BL will
not trigger on alarm.