Okolab H301-EC-LG-BL User manual

H301-EC-LG-BL User Manual
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H301-EC-LG-BL
Compatible with the following Z-Piezo stages
Compatible with the following Okolab Controllers
•LEICA super Z Galvo stage (SP5/SP8)
•H301-T-UNIT-BL-PLUS
•UNO-COMBINED-CONTROLLER
•H401-T-DUAL

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Index
1. Components and dimensions................................................................................................... 3
2. Sample Holders................................................................................................................... 4
2.1 Available Sample Holders....................................................................................................... 4
3. Insertion of the Sample Feedback Temperature Sensor.................................................................. 5
4. Insertion of Sample Holder into Chamber ................................................................................... 5
5. Working with 35 or 60 mm Petri Dish........................................................................................ 5
6. Working with 1x3’’ and 1x2’’ chamber slides .............................................................................. 7
7. Connecting the Gas Supply ..................................................................................................... 8
8. Working with Perfusion ......................................................................................................... 8
9. Connecting the Chamber with Super Z-Galvo stage ....................................................................... 9
10. Cleaning ..........................................................................................................................10

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1. Components and dimensions
H301-EC-LG-BL includes the following components:
•Chamber main body with embedded temperature sensor.
•Heated glass lid with Indium Tin Oxide (ITO) conductive coating and embedded temperature sensor.
•Total chamber weight: about 130 g. For details, see the table at page 4.
Distance between focal plane
and upper side of the glass lid
Chamber top view
Lid
Chamber Base
Sample Holder-Order Separately
Figure 1. H301- EC-LG-BL - Components and Dimensions.

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2. Sample Holders
2.1 Available Sample Holders
The following sample holders are available.
NOTE: Please contact [email protected] if you cannot find the sample holder you are looking for. We are constantly
adding new inserts to the list.
H301-EC-LG-1x35
#1 35mm Petri-dish holder
H301-EC-LG-1xGS
#1 standard chamber slide holder
H301-EC-LG-1x60
#1 60mm Petri-dish holder
H301-EC-LG-1xLABTEK
#1 Lab-TeK 1”x2” chambered cover glass holder
H301-EC-LG-1xLABTEK-II
#1 Lab-TeK II1”x2” chambered cover glass holder
H301-EC-LG-1xGS
For #1 standard chamber slide
holder
H301-EC-LG-1x35
For #1 35mm Petri-dish holder
H301-EC-LG-1x60
For #1 60mm Petri-dish holder
H301-EC-LG-1xLABTEK
For #1 Lab-Tek 1”x2”
chambered cover glass holder
1xLABTEK-II-M single
For #1 Lab-Tek II
1”x2”chambered cover glass
holder
Figure 2.Available sample holders.
H301-EC-LG-BL has a different weight in relation to sample holder used. The following table shows the weight of all
possible configurations.
H301-LG-BL+SAMPLE HOLDER
WEIGHT (g)
…………………….H301-EC-LG-1x35
130.7
…………………….H301-EC-LG-1xGS
127.4
…………………….H301-EC-LG-1xLABTEK
125.1
…………………….H301-EC-LG-1xLABTEK-II
133.5
.................H301-EC-LG-1x60
129.5

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3. Insertion of the Sample Feedback Temperature Sensor
Insert the Sample Feedback Temperature Sensor through the dedicated opening, labeled ‘T Sensor’, located on the
H301-EC-LG-BL base (see Figure 3, Frontal and 3D views).
1.Frontal view 2.3D view
Temperature Sensor dedicated opening
Temperature Sensor dedicated opening
Figure 3. Insertion of the temperature sensor inside the chamber.
4. Insertion of Sample Holder into Chamber
Sample holders fit into the chamber base and are held in place by 4 screws (Slotted Pan Head Screw M2x3). To intro-
duce a sample holder with the proper orientation and to screw it to the chamber, see Figure 4.
Captive Screw
Figure 4. Introduction of the Specimen Holder inside the Chamber Base.
5. Working with 35 or 60 mm Petri Dish
Flat springs prevent movement of 35 and 60 mm dishes inside the sample holder (See Figure 5 and Figure 6).

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A
A
12
Figure 5. Plate adapter for 35mm Petri-dish.
21 A
A
Figure 6. Plate adapter for 60 mm Petri-dish.

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6. Working with 1x3’’ and 1x2’’ chamber slides
Flat springs prevent movement of 1’’x 3’’ and 1’’x 2’’ chamber slides inside of the sample holder.
A
A
12
Figure 7. Plate adapter for Lab-TeK 1”x2” chambered cover glass.
1 2
A
A
Figure 8. #1 Plate adapter for Lab-TeK II1”x2” chambered cover glass.

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7. Connecting the Gas Supply
A single silicon tubing carries output gas from the Okolab Gas Controller to the H301-EC-LG-BL. Silicon tubing connects
to a gas input - brass opening - located on a corner of the H301- EC-LG-BL. See Figure 9. Connect by gently pushing
silicon tubing onto brass opening.
Gas Input
Figure 9.Connection with gas supply.
8. Working with Perfusion
The H301-EC-LG-BL features 4 perfusion holes on the side for the insertion of perfusion tubing up to 2.0 mm in outer
diameter. Small screws plug the perfusion holes when not in use. (Grub screws M2.5x3). Remove small screws as nec-
essary before introducing perfusion tubing.
Figure 10 shows the location of perfusion holes.
1.Frontal view 2.3D view
Perfusion dedicated opening
Figure 10. Perfusion

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9. Connecting the Chamber with Super Z-Galvo stage
H301-EC-LG-BL fits on the Super Z-Galvo stage. It can be screwed on the Galvo Stage as shown in Figure 11; place the
chamber on the stage and screw the four slotted head pan screws. Screws housings are indicated with letter A in Fig-
ure 11.
Captive Screw
A
A
Figure 11. How to fix the H301-EC-LG-BL on the Super Z Galvo stage

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10. Cleaning
•Turn the system off and pull the mains plug out the socket
•Remove the lid from the chamber main body, and keep it separate from the chamber main body while the
chamber cools down.
•To clean the body and the glass lid of the chamber, wipe with a soft micro-fiber cloth. For stubborn smudges,
you can damp the soft micro-fiber cloth with ethyl alcohol (product code UN1170). Do not put any liquid di-
rectly on the chamber. While cleaning the glass lid, do not apply strong force to the surface of the glass lid
because it can be damaged.
H301-EC-LG-BL_User Manual Rev 03_Published in January 2020