Olympus BX61WI User manual

INSTRUCTIONS
BX61WI
FIXED-STAGE MOTORIZED
UPRIGHT MICROSCOPE
A X 6 1 1 8
This instruction manual is for the Olympus Fixed-Stage Motorized Upright Microscope Model BX61WI.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of
this microscope, we recommend that you study this manual thoroughly before operating the micro-
scope. Retain this instruction manual in an easily accessible place near the work desk for future
reference.

BX61WI
CONTENTS
4
5-11
12-13
14-23
IMPORTANT — Be sure to read this section for safe use of the equipment. —
1 MODULE NOMENCLATURE
2 CONTROLS
3
4 USING THE CONTROLS
TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE
4-1 Microscope Frame .................................................................................................................................................................. 14-15
4-2 Focusing Block ............................................................................................................................................................................ 15-16
1Voltage Indication 2Transmitted/Reflected Light Switch
3Light Preset Switch 4Using the Filter Turret
4-3 Stage (IX-SVL2).............................................................................................................................................................................. 17- 18
4-4 Revolving Nosepiece.................................................................................................................................................................... 18
4-5 Observation Tube ..................................................................................................................................................................... 19-20
1Adjusting the Interpupillary Distance 2Adjusting the Diopter
3Using the Eye Shades 4Using Eyepiece Micrometer Disks
5Selecting the Light Path of Trinocular Tube
1Switching the Objectives
1
Replacing the Focus Adjustment Knob 2F/C Button
3Objective Up/Down Buttons 4Objective Escape/Return Button
5Using the Frost Filter Insertion/Removal Lever
1-3
Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to
assemble the microscope yourself, please read Chapter 9, “ASSEMBLY” (pages 42 to 49) carefully. For the modules
provided with instruction manuals, also read the assembly procedures in their instruction manuals.
1Placing the Specimen 2Moving the Specimen
3Setting the Grounding 4 Adjusting the X-Axis/Y-Axis Knob Rotation Tension
5Using the Light Shielding Sheet 6Lowering the Stage Height
4-6 Condenser.......................................................................................................................................................................................... 21-22
1Centering the Condenser 2Oblique Illumination (WI-OBCD)
4-7 Water Immersion Objectives ............................................................................................................................................ 23
1Using Water Immersion Objectives (Water Immersion Cap)

5 OTHER OBSERVATION METHODS
5-1 Differential Interference Contrast Observation............................................................................. 24-28
1Attaching the Analyzer 2Attaching the Polarizer
3Attaching the DIC Prisms (for Revolving Nosepiece)
4
Attaching the DIC Prisms (for Condenser)
5Adjusting the Polarizer Position
6Observation Method
5-2 Reflected Light Fluorescence Observation ................................................................................................ 29
5-3
Infrared Light (IR)/Differential Interference Contrast (DIC) Observation .............. 29-32
1Introduction 2Attaching the IR Modules
3DIC Observation Using IR
5-4 Macro Reflected Light Fluorescence Observation ................................................................ 33-35
1Introduction 2Attaching the Modules
3Filter Characteristics of Fluorescence Mirror Units
4 Fabricating Optional Mirror Units
6 TROUBLESHOOTING GUIDE
7 SPECIFICATIONS
8 OPTICAL CHARACTERISTICS
9
36-38
39-40
41
42-49
ASSEMBLY — See this section for the replacement of the light bulb. —
24-35

1
BX61WI
Fig. 1
IMPORTANT
This microscope employs a UIS (Universal Infinity System) optical design, and should be used only with
modules designed for the BX2 series (which belong to the Olympus BX series).
For the applicable modules, please consult Olympus or the latest catalogues. Less than optimum
performance may result if inappropriate module combinations are used.
Configuration of Instruction Manuals
Since this microscope is expandable to a variety of systems, separate instruction manuals are prepared so
that the user has to read only the manuals according to the user’s own system.
Manual Name Main Contents
BX61WI Observation procedures including transmitted light brightfield, DIC
and IR observations
BX-UCB/U-HSTR2 Functions of the Control Box (incorporating the power supply)
and Hand Switch
BX2 Software for PC (CD-ROM)
* BX2-BSW Ver. 03.01 or higher
Methods of PC control of functions
* Normal operation is not available unless the specified software is used.
TH4 External halogen lamp power supply
BX-RFAA/BX-URA2/BX-RFA Reflected light fluorescence observation procedure
U-FWT/FWR/FWO Motorized filter wheels (The U-FWT cannot be used with this
microscope.)
SAFETY PRECAUTIONS
1. Culture liquid or water spilt on the stage, condenser or microscope may
damage the equipment. Immediately wipe the liquid or water off if it is
spilt on them.
2. It a foreign object is caught during motorized focusing operation, there
will be an error in the focusing block and the motorized focusing opera-
tion will be suspended.
Recovery procedure
· If there is no error in motorized operation, the caught object can be
removed by turning the focusing knob.
· If there is an error in motorized operation, the focusing knob becomes
inoperable. Disassemble the relevant modules to remove the caught
object. Replace the relevant modules afterwards.
· Turn off the power and then on again. The system will restart unless
there is a malfunction in the motor.
1
2
3. Emergency stop of focus operation is possible by turning the focus
adjustment knob (or dial) on the microscope frame or on the U-FH (in
either direction), or by pressing the FOCUS control button ( , , F/C or
ESC), after focus operation has been activated (except when data is
being downloaded to a PC).
When the BX-UCB control box’s main switch is set to “ I” (ON), the focus
operates automatically (the objective raises once and then returns to the
original position) for initialization. (It takes about one minute.)
If the above emergency procedure is performed during this automatic
focus operation, the microscope stops operating. Should this happen,
set the main switch to “ ” (OFF) and then “ I” (ON) again.
4. When moving the microscope, disconnect the reflected light illuminator,
observation tube and transmitted light lamp housing and carefully carry
the microscope by the base (front edge) 1and the grasping part on the
rear of the arm 2as shown in Fig. 1. (Weight: approx. 15 kg.)
Also be careful against slipping of hands during carrying.
# Damage to the microscope will occur if you grasp it by other parts
including the stage, focus adjustment knob, etc.

2
Fig. 2
6. When installing the microscope, route the power cord away from the
lamp housing. Should the power cord come in contact with the hot lamp
housing, the power cord could melt and cause electric shock.
7. To avoid potential shock hazards and burns when replacing the light
bulb, set the main switch ³ to “ ” (OFF) then disconnect the power cord
from the wall outlet in advance. Whenever you replace the bulb during
use or right after use, allow the lamp housing | and bulb to cool before
touching. (Fig. 2)
Designated
Bulbs
12V100WHAL (PHILIPS 7724)
12V50WHAL-L (LIFE JC)
# The microscope also incorporate a fuse (this should be
replaced by the manufacturer or an Olympus-authorized
agent).
8. Always use the power cord provided by Olympus. If the proper power
cord is not used, product safety performance cannot be warranted.
9. Always ensure that the grounding terminal of the microscope and that
of the wall outlet are properly connected. If the equipment is not grounded,
Olympus can no longer warrant the electrical safety performance of the
equipment.
Never insert metallic objects into the air vents of the microscope frame
as this could result in electrical shock, personal injury and equipment
damage.
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol Explanation
Indicates that the surface becomes hot, and should not be touched with bare hands.
Before use, carefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
l
Warnings
Warning engraving is placed at parts where special precaution is required when handling and using the microscope.
Always heed the warnings.
Warning engraving
position
Lamp housing (U-LH100-3/U-LH100IR)
(Warning against high temperature)
3
4
5. The surfaces of the lamp housing on the rear of the microscope will
become extremely hot during operation. When installing the microscope,
make sure to allow ample free space (10 cm or more) around and in
particular above the lamp housing.
10.

3
BX61WI
1Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. The U-SWTR-2 super-widefield observation tube (FN 26.5) cannot be used with the BX61WI microscope.
3. The BX61WI microscope can be used with an intermediate attachment (such as a BX-RFAA, BX-URA2 or BX-RFA reflected
light illuminator, U-ECA or U-CA magnification changer, etc.).
Two intermediate attachments can be used only in the following conditions:
· The U-CA or U-ECA magnification changer or U-FWO filter wheel can be mounted as the second attachment.
· When a TV adapter with 1X or higher power is used, 2/3-inch CCD TV observation is possible.
· The peripheral areas of the field of view may be obscured or cut off in binocular observation using the U-TR30-2, U-ETR3
or U-TR30IR (FN 22) super-widefield observation tube.
4. In IR (infrared) observation, the U-CA or U-ECA magnification changer can be used only when the U-ETR3 or U-TR30IR
observation tube is used.
5. In photomicrography with visible light, correct exposure may be impossible if the microscope is set for IR observation.
Be sure to engage the provided IR cut filter (light blue) before photomicrography.
6. When the XLUMPlanFl20XW objective is used, only the U-TV1X, U-TVCAC, U-PMTVC2XIR or U-PMTVC4XIR TV adapter
can be used.
7. Do not attempt to remove or loosen the click springs and screws. Otherwise, Olympus can no longer warrant the
performance of the microscope.
The clicking force of the revolving nosepiece has been set weak in order to reduce vibrations during objective switching.
To reproduce the correct click position, switch the objectives gently by operating the lever.
8. Caution for use of the U-ETR3 upright trinocular tube:
When the aperture stop of the condenser is reduced using a reflected light fluorescence illuminator and the LUMPlanFl60XW
objective, part of the observed field of view may be obscured slightly. This is due to the reduction of the light intensity in
the field of view due to the narrow aperture and is not due to a defective optical adjustment of the microscope.
This phenomenon does not affect the photomicrography or TV camera light path.
2Maintenance and Storage
1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly
moistened with a mixture of ether (70%) and alcohol (30%).
#To clean the extremity of the water immersion objective, use neutral detergent.
Do not use the ether/alcohol mixture for cleaning, for these will deform the electrically insulated section of the
extremity.
Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep
these chemicals away from open flames or potential sources of electrical sparks –– for example, electrical equip-
ment that is being switched on or off. Also remember to always use these chemicals only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean
them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Never attempt to disassemble any part of the microscope.
4. When not using the microscope, make sure to set the main switch to “ ” (OFF), confirm that the lamp housing is cool
enough and cover the microscope with the provided dust cover.
3Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).

4
MODULE NOMENCLATURE
}The modules shown below are only the representative modules. As there are other modules which can be combined with
the microscope but are not shown below, please also refer to the latest Olympus catalogues or your dealer.
CAUTION The Z board combined with the BX61WI must always be the U-ZPCB(T2). Also note that the DIP switch
setting should be changed when the Z board is used (see page 44).
Reflected Light Mercury Lamp Housing
· U-LH100HG
· U-LH100HGAPO
Transmitted Arm BX-ARM
or Reflected Light Fluorescence Illuminator
· BX-RFAA
· BX-URA2
· BX-RFA
Straight Photo Tube U-SPT
Trinocular Observation Tube
· U-TR30-2 (FN 22)
· U-TR30IR (FN 22)
· U-ETR3 (FN 22)
Revolving Nosepiece
· WI-SRE3
· WI-SNPXLU2
· U-SLRE
· WI-SSNP
(with revolving arm)
Revolving Arm WI-NPA
Cross Stage IX-SVL2
or bridge stage WI-XYS
Fixed-Stage Adapter
WI-FSH
Condenser
· WI-UCD
· WI-DICD
· WI-OBCD
· U-UCD8
· U-SC3
· U-AAC
· U-AC2
Focus Adjustment Knob Unit U-FH
Focus Adjustment Knob Interface
U-IFFH
Microscope Frame BX61WIF
Power Supply Unit
(for 100 W Mercury
Burner)
Transmitted Light Lamp Housing
· U-LH100-3
· U-LH100IR
Control Box BX-UCB
The U-ZPCB(T2) Z Board
can be mounted here.
Hand Switch U-HSTR2
Objectives
· MPlan5X
· UMPlanFl10XW/20XW
· LUMPlanFl40XW
· LUMPlanFl40XW/IR-2
· LUMPlanFl60XW
· LUMPlanFl60XW/IR-2
· LUMPlanFl100XW
· XLUMPlanFI20XW (exclusively for use with the WI-SNPXLU2)
· XLFluor2X/340 (exclusively for use with the U-SLRE)
· XLFluor4X/340 (exclusively for use with the U-SLRE)

5
BX61WI
CONTROLS
}If you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 42 to 49).
Interpupillary distance scale (Page 19)
}The descriptions on the left side of the microscope frame, filter turret, revolving nosepiece, condenser, etc. will be given in the
subsequent pages.
Diopter adjustment ring (Page 19)
Reflected light fluorescence illuminator
(See the separately provided instruction manual.)
Stage center plate
Y-axis knob (Page 17)
X-axis knob (Page 17)
Centering knobs
(Storage position)
Light intensity control buttons
(Page 14)
Light path selector knob (Page 20)
Reflected light mercury lamp housing
(See the separately provided instruction
manual.)
Allen screwdriver
(Storage position)
Lamp voltage indicator
(Page 14)
Mercury lamp power
supply unit
(See the separately
provided instruction
manual.)
Transmitted/reflected
light switch (Page 14)
(For halogen bulb)
PHOTO indicator
(Page 14)
Control Box BX-UCB
(See the separately provided
instruction manual.)
Focus adjustment dial (Page 15)
Objective down button (Page 16)
Objective up button (Page 16)

6
Left Side View of Microscope Frame
Focus adjustment knob (Page 15)
Filter Turret
Polarizer positions* (Page 24)
* Accepts the combination of a
polarizer push ring and a 32 mm
filter. The thickness should be no
more than 6 mm.
Objective escape/return button (Page 16)
F/C button (Page 16)
F: Fine adjustment
C: Coarse adjustment
Frost filterinsertion/removal lever (Page 16) Light Preset switch (Page 14)
Lamp ON-OFF switch
Accepts a 32PO or 32POIR polarizer.
Indication label
attaching frame
Filter positions
Accepts 32 mm filters with
thickness of no more than
6 mm.
Polarizer clamping knob (Page 24)
Polarizer rotating dials
(Page 28)
Slider insertion/removal stopper (Page 15)
Filter frame reinforcing ring
(Page 46)
Filter slider (Page 15)
IR filter insertion position
Accepts a 32BP775, 32IR900 or a 32 mm filter with
thickness of no more than 5.3 mm.

7
BX61WI
Revolving Arm WI-NPA
# Note that the revolving arm can be mounted only before the reflected light illuminator or transmitted light arm and
the IX-SVL2 stage are mounted (page 45).
}This revolving arm accepts the U-SLRE, WI-SNPXLU2 or WI-SRE3 revolving nosepiece.
Light shielding tube retaining screw
Sliding Revolving Nosepiece U-SLRE
Switching lever
Single-Position Revolving Nosepiece XLU
WI-SNPXLU2
DIC mount hole
(Page 25)
Swinging Revolving Nosepiece WI-SRE3
Swing lever
Revolving nosepiece mount dovetail
Revolving nosepiece clamping screw
Grounding screw hole (M3)
Microscope frame mount dovetail
XLFluor objective mount screw
M 34 mm, pitch 1 mm UIS objective
mount screw
RMS Adapter WI-RMSAD
XLU objective mount screw
M 25 mm, pitch 0.75 mm
UIS objective mount screw
Drop prevention screw
Centering screws
Drop prevention screw
DIC mount hole
(Page 25)
UIS objective mount screws
Confocality correction washer
(Thickness 10, 30 and 50 µm, x 3 each)
Centering knobs

8
Long-WD Universal Condenser WI-UCD
Turret (4 positions)
Aperture iris diaphragm lever (Page 22)
Long-WD DIC Condenser WI-DICD
Aperture iris diaphragm
lever (Page 22)
Differential Interference Contrast Prisms (For Condenser)
}The WI-UCD condenser accepts two large and two small DIC prisms while the WI-DICD condenser accepts one large or
small DIC prism.
When selecting the brightfield (BF) light path using the WI-UCD, leave one DIC prism (large) mount position empty.
· U-LDPW10H
· U-LDPW20H
Quarter-wave plate rotation
ring (Page 28)
Optical element index
mount (Page 26)
Quarter-wave plate
clamping knob
DIC prism replacement cover
Quarter-wave plate
clamping knob
Quarter-wave plate rotation ring
(Page 28)
DIC prism (Large) mount position
DIC prism (Small) adapter
Index
Positioning indices
· U-LDPW40H
· U-LDPW60H
· U-LDPXLU20HR
Index

9
BX61WI
Long-WD Oblique Condenser WI-OBCD
Aperture iris diaphragm lever (Page 22)
High-Resolution DIC Prism A WI-DICTHRA2
DIC Prism WI-DICT2
}This prism can be mounted in the DIC prism position
of the WI-SNPXLU2 or WI-SRE3.
Applicable condensers
WI-DICTHRA2: WI-UCD, W-DICD
WI-DICT2: U-UCD8
Positioning pin
Condensers and Applicable Objective Magnifications
Condenser Applicable Objective Magnification
WI-UCD
WI-DICD
WI-OBCD
5X or more
U-UCD8
U-SC3 2X or more
U-AAC 10X or more
U-AC2 5X or more
Oblique iris insertion/removal knob (Page 22)

10
}The following modules are required to eliminate electrical noise, which may affect the potential measurement data during
patch clamping.
Halogen Lamp Power Supply TH4 (See the separately provided instruction manual for details.)
Hand Switch TH4-HS
Focus Adjustment Knob Interface U-IFFH
Microscope FRAME connector
U-FH connector
BX-UCB connector

11
BX61WI
Focus Adjustment Knob Unit U-FH
The button functions described below are those when the microscope is used stand-alone.
The buttons functions inside are the initial setups for PC control (remote operation).
The button functions can also be assigned as required by the user. For the assignment, refer to the instructions for the
BX2-BSW BX Software (Ver. 03.01 or higher).
After setting up the button functions, attach the provided stickers near the buttons. For the function abbreviations and
symbols on the stickers, see the following table.
Objective escape/return button (Page 16)
Lamp ON/OFF
Connector
Objective up button (Page 16)
Objective escape/shelter
Focus adjustment knob
Objective down button (Page 16)
F/C
F/C button (Page 16)
Focusing motor ON/OFF
Transmitted/reflected light
switch button (Page 14)
Option
Abbreviations & Symbols Function Note
Fine/Coarse switching
Lamp ON/OFF
Set/cancel photo voltage
Focus adjustment knob FRAME/U-FH
Z-focusing motor ON/OFF OFF: Electrical noise reduction
Escape/return objective
Shutter IN/OUT
Condenser top lens IN/OUT Not used with the BX61WI.
Up/down operation for brightness ad-
justment, objective, etc.
The function name can be written
in the blank area using an oil-ink
pen.
Left/right operation of mirror unit, filter
wheel, etc.
Connect to the U-IFFH.

12
TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic
observation method of this microscope. The operating procedures for DIC observation, fluorescence DIC observation and
IR DIC observation will be described separately in Chapter 5, “OTHER OBSERVATION METHODS” on page 24.
(Controls Used) (Page)
Tip for microscope operation
In patch-clamp testing, switch the microscope controls cautiously and gently so that the patch
electrodes do not slip off.
Set the main switch to “ I” (ON). @Main switch
²Lamp ON-OFF switch
Select the transmitted light illumination and
adjust its brightness.
³Transmitted/reflected light switch (P. 14)
|Light intensity control buttons (P. 14)
Disengage the filter from the light
path. 5Filter turret (P. 15)
Select the binocular light path. …Light path selector knob (P. 20)
Place the specimen on the stage. 7X-axis and Y-axis knobs (P. 17)
Engage a low-magnification objective in the
light path. 8Swing lever (P. 18)
Bring the specimen in focus. 9Objective up/down buttons, focus
adjustment knob/dial
(P. 16)
Adjust the brightness. |Light intensity control buttons (P. 14)
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
aBinocular tube (P. 19)
bDiopter adjustment ring (P. 19)
cCondenser height adjustment knob (P. 21)
dCondenser centering knobs (P. 21)
eField iris diaphragm ring (P. 21)
Engage the objective to be used in the light
path and bring the specimen in focus.
‡Swing lever (P. 18)
9Objective up/down buttons, focus
adjustment knob/dial
Adjust the aperture iris and field iris diaphragms.
(P. 16)
fAperture iris diaphragm lever (P. 22)
eField iris diaphragm ring (P. 21)
Engage the required filters. 5Filter turret (P. 15)
Adjust the brightness. |Light intensity control buttons (P. 14)
Start observation.

13
BX61WI
(Note) Controls with numbers inside can perform the same
functions as the controls with numbers inside ¦.
However, the function of control is not available when
the U-FH is in use.
6
3
9
1
} Make a photocopy of the observation procedure pages and post it near your microscope.
b
a
8
7
cdf
5
e
4
9
3
9
2
Control Box BX-UCB
* The U-ZPCB (T2) Z board can
be mounted here.
See page 44 for the setup and
installation of the Z board.
9
9
9

14
Fig. 3
Fig. 4
USING THE CONTROLS
4-1 Microscope Frame
1Voltage Indication (Fig. 3)
1. Press the light intensity control button 1to increase the voltage and
make illumination brighter.
Pressing the button 2makes the illumination darker.
2. The numerals to the left of the lamp voltage indicator LEDs 3indicate
the reference values of the voltages.
# The LEDs may turn off (temporarily) when the motor is driven, but the
illumination intensity even if this occurs.
2Transmitted/Reflected Light Switch (Fig. 3)
}The same effect as this switch can also be obtained using the switch on
the U-FH focus adjustment knob unit (see page 15).
The illumination can be switched between the transmitted light and
reflected light by pressing the transmitted/reflected light switch 4.
: Reflected light illumination
: Transmitted light illumination
The LED indicator of the selected illumination lights.
3Light Preset Switch (Fig. 4)
}The Light Preset switch sets the light intensity voltage to a voltage suitable
for color photography* (the factory default is 9 V) regardless of the current
setting of the light intensity control buttons.
* Achieved by engaging the LBD filter.
Setting the Desired Brightness (Figs. 3 & 4)
1. Press the Light Preset switch 5. The PHOTO indicator LED 6lights up.
2. Press one of the light intensity control buttons 1 and 2to set the desired
brightness.
3. Press the Light Preset switch again. The LED turns off, the brightness
returns to the original brightness but the setting made above is stored in
memory.
4. Hereafter, pressing the Light Preset switch sets the brightness to the
intensity value set in step 2 above.
# Be sure to return to the 9 V setting when performing color photogra-
phy.
}When the Light Preset switch 5is pressed, an announcement tone (short
beep) is generated at the 9 V position of the switch.
}When the external TH-4 power supply unit is used instead of the
microscope’s built-in power supply circuitry, refer to the instruction manual
provided with the TH4.
1
2
3
46
5

15
BX61WI
Fig. 5
Fig. 6
4Using the Filter Turret (Fig. 5)
}Filters with a diameter of 32 mm can be inserted in positions 1to 4.
1. Filter positions 1 2 are rotatable. When the 32PO or 32POIR polarizer is
placed in either position, the polarizer can be fixed by a push ring.
}When filter position 1is engaged in the light path, the polarizer clamping
knob 5comes at the front where the operation is easy.
2. Filter position 3accepts any type of 32 mm filter.
# When using two filters together, the thickness of the lower filter should
be no more than 2 mm. Otherwise, the upper filter may drop during
rotation.
3. Filter position 4accepts the 32BP775 or 32IR900 IR filter. As the filter
cannot be inserted unless the filter slider is removed, remove it by releasing
the insertion/removal stopper below the slider and loosening the slider
clamping screw using the provided Allen screwdriver.
4-2 Focusing Block
}The same effect as the focus adjustment knob on the microscope frame can also be obtained using the U-FH focus
adjustment knob unit. However, when the microscope is used stand-alone while the cable to the U-FH is connected, the
focus adjustment is available only from the focusing knob on the U-FH.
Objective down button
Focus Adjustment Knob Unit U-FH
1Replacing the Focus Adjustment Knob (Fig. 6)
}The focus adjustment knob is installed on the right side of the microscope
when it is shipped from the factory. (Detachable)
1. Loosen the clamping screw 1with the Allen screwdriver and remove the
focus adjustment knob 2.
2. Remove the seal from the focus adjustment knob screw hole on the
other side and attach the knob by reversing the removal procedure.
3. Attach a provided seal on the screw hole 4of the removed focus adjust-
ment knob 3.
1
2
3
4
5
F/C button
Transmitted/reflected
light switch button
Objective up button
Objective escape/return button
Focus adjustment knob
1
32
4

16
Fig. 7
Fig. 8
Fig. 9
Fig. 10
2F/C Button (Fig. 7)
}The F/C button switches the function of the focus adjustment knob 1
and focus adjustment dial between F (fine) and C (coarse). (For safety, the
F/C button is set automatically to F at the moment the main switch of the
BX-UCB control box is set to “ I” (ON).)
· Each press of the F/C button 2switches F and C alternately.
Objective fine movement: 0.1 mm per turn
Objective coarse movement: 0.3 mm per turn
3Objective Up/Down Buttons (Fig. 8)
# When the objective is lowered, be careful not to let it collide with the
specimen.
· Press the objective up button 1to raise the objective and press the
objective down button 2to lower the objective.
· The stroke is 25 mm.
4Objective Escape/Return Button (Fig. 9)
When replacing the specimen, press the Objective Escape/Return button
1The objective will rise by 5 mm (in 1 sec.). Pressing the button again
returns the objective to the original height.
# Even after escaping, care is still required during objectives switching,
for the objective may still interfere with the edge of the container. If this
happens, raise the objective higher using the objective up button.
5 Using the Frost Filter Insertion/Removal Lever
(Fig. 10)
}Low observation light can be brightened by turning the frost filter insertion/
removal lever 1which controls the built-in frost filter, in the direction of the
arrow. However, although the brightness is increased, irregularity in lighting
may also increase.
12
1
2
1
1