Olympus CX43-RFAB User manual

CX43-RFAB
This instruction manual is for the B excitation fluorescence illuminator.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this
product, we recommend that you study this manual thoroughly before operating this product, and
always keep this manual at hand when operating this product.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
INSTRUCTIONS
B excitation fluorescence illuminator
A X 8 9 1 6
Optical Microscope Accessory

In accordance with European Directive on Waste Electrical and Electronic Equipment, this symbol indicates that the
product must not be disposed of as unsorted municipal waste, but should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in your country.
For Korea only
B급 기기 (가정용 방송통신기자재)
이 기기는 가정용(B급) 전자파적합기기로서 주로 가정에서 사용하는 것을
목적으로 하며, 모든 지역에서 사용할 수 있습니다.
NOTE: This product has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC
Rules. These limits are designed to provide reasonable protection against harmful interference when the product is operated
in a commercial environment. This product generates, uses, and can radiate radio frequency energy and, if not installed and
used in accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this product in a residential area is likely to cause harmful interference in which case the user will be required to
correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance could void the user’s
authority to operate the product.
This product is applied with the requirements of standard IEC/EN61326-1 concerning electromagnetic compatibility.
• Immunity Applied to industrial and basic environment requirements.

CX43-RFAB
Introduction ...............................................................................................................................................1
Safety precautions ..............................................................................................................................2
1 Nomenclature of microscope operating portions .............................................6
2 Reflected fluorescence observation method ........................................................7
3 Operation procedures ................................................................................................................8
1 Operating the B excitation fluorescence illuminator ..............................................................................................8
2 Escaping the condenser top lens ...........................................................................................................................................9
4Assembly ................................................................................................................................................10
1 Attaching the B excitation fluorescence illuminator ................................................................................................10
2 Connecting the AC adapter and power cord ................................................................................................................11
3 Attaching the hook stick holder ................................................................................................................................................12
5 Specifications .....................................................................................................................................13
Contents

CX43-RFAB
1
If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
product may also be damaged. Always use the product according to this instruction manual.
Introduction
The following symbols are used in this instruction manual.
CAUTION : Indicates a potentially hazardous situation which, if not avoided, may result in
minor or moderate injury.
NOTE
: Indicates a potentially hazardous situation which, if not avoided, may result in
damage to the product or other property.
TIP
: Indicates the useful knowledge or information for use.
This product is used for a reflected light fluorescence observation in combination with the biological
microscope CX43.

2
Safety precautions
CAUTION - Electric safety -
Always use the AC adapter and power cord provided by Olympus.
If the proper AC adapter and the power cord are not used, the electric safety and the EMC (Electro-Magnetic Compat-
ibility) performance of the product cannot be assured. If no power cord is provided, please select the proper power
cord by referring to the section “Proper selection of the power cord” at the end of this instruction manual.
Always connect the ground terminal.
Check that the ground terminal of the power cord and that of the power outlet are connected properly. If the product is
not grounded, our intended electric safety and EMC performance of the product cannot be assured.
Do not use this product near the sources of strong electromagnetic radiation.
Proper operation may be interfered. Be sure to evaluate the electromagnetic environment before operating the product.
Disconnect the power cord in case of emergency.
In case of emergency, disconnect the power cord from the power cord connector of the product or from the power
outlet.
Install the product at the location where you can reach the power cord connector or the power outlet with your hand to
disconnect the power cord immediately.
This product complies with the emission and immunity requirements described in IEC61326 series.

CX43-RFAB
3
CAUTION - LED (light emitting diode) -
Do not look directly at the light from LED for a long time.
If you feel that the light from LED is too bright during observation, adjust the light intensity using the brightness adjustment
knob before continuing the observation. The LED built in this product is basically eye-safe. However, do not look directly
at the light from LED for a long time while feeling too bright, since it may cause damage to your eyes.
CAUTION - Light from the objective -
Do not look directly at the light coming out from the objective or the light reflected from the specimen.
Be careful about the light coming out from the objective, since not only visible light but also light of invisible wavelengths
(such as ultraviolet and infrared) may be emitted depending on the illumination methods.

4
CAUTION - Safety symbols -
The following symbols are attached to the product.
Study the meaning of the symbols and always use the product in the safest possible manner.
Symbol Meaning
Indicates a non-specific general hazard. Follow the description given after this symbol
or in the instruction manual.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Handling precautions
· This product is a precision instrument. Handle it with care and avoid subjecting it to a sudden or
severe impact.
· Never disassemble any part of the product. Otherwise, failure could be caused.
· Do not carry the microscope keeping the AC adapter connected to the microscope. The AC adapter or
the microscope may be damaged by hitting the output connector of the AC adapter.
NOTE

CX43-RFAB
5
Maintenance and storage
1. Do not leave stains or fingerprints on the lenses and filters. If they get dirty, blow away dust with a commercially avail-
able blower and gently wipe the lens or filter with a piece of cleaning paper (or clean gauze). Only when cleaning
fingerprints and oil stains, slightly moisten a piece of cleaning paper with commercially available absolute alcohol
and wipe them off with it.
Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away
from open flames or potential sources of electrical sparks. For example, the electrical equipment that
is switched ON and OFF may cause the ignition of a fire. Also, always use absolute alcohol only in a
well-ventilated room.
2. Wipe the portions other than the lens with a dry soft cloth. If the dirt cannot be removed by dry-wiping, moisten a soft
cloth with diluted neutral detergent and wipe the dirty surface with it.
Do not use organic solvents because they may deteriorate the coated surface or plastic parts.
3. When disposing of this product, be sure to follow the regulations and rules of your local government. Contact Olympus
for any questions.
CAUTION
NOTE

6
1 Nomenclature of microscope operating portions
1
* : For operating procedures, refer to the instruction manual of the microscope frame (CX43).
Y-axis knob
*
Revolving nosepiece
*
X-axis knob
*
Main switch
*
: Power is ON.
: Power is OFF.
Fine focusing knob
*
Coarse focusing knob
*
Aperture diaphragm
lever
*
Specimen holder
*
Condenser turret
*
Brightness
adjustment knob *
Diopter
adjustment ring
*
Binocular portion*
Condenser top lens
(see page 9)
Aperture diaphragm
Aperture diaphragm
lever
*
B excitation fluorescence
illuminator (see page 8)
Y-axis knob
*
X-axis knob
*
2c
49
6
7
5a
8
d
3
3
3
5af
5af
bef
The number indicating the operation portion corresponds to the observation method described on the next page.
1
Main switch
*

CX43-RFAB
7
For reflected fluorescence observation, the B excitation fluorescence illuminator is necessary.
(For attaching procedures, see “4Assembly” on page 10.)
If the analyzer for simple polarization and the polarizer are engaged in the light path, remove them from the light path.
2 Reflected fluorescence observation method
Place the specimen on the stage.
Engage the 10X objective in the light path.
Bring the specimen into focus and
adjust the brightness.
Set the main switch of the microscope frame
to (ON).
Select BF (brightfield observation).
Adjust the interpupillary
distance.
Adjust the diopter.
1
2
3
4
5
6
7
Observation
Remove the top lens
of the condenser
from the light path.
Select FL (fluorescence observation).
Bring the specimen into focus and
adjust the brightness.
Bring the specimen into focus and
adjust the brightness.
Engage the B excitation fluorescence
illuminator in the light path.
Set the main switch of the B excitation
fluorescence illuminator to (ON).
Engage the objective you use in the light path.
b
a
c
f
e
d
9
Engage the objective for phase contrast
in the light path.
8

8
1 Operating the B excitation fluorescence illuminator
For attaching and removing the B excitation fluorescence
illuminator, see page 10.
Set the main switch a of the B excitation fluorescence illuminator
to (ON).
When the B excitation fluorescence illuminator is not in
use, slide it in the arrow direction until it touches the end to
remove it from the light path.
· When attaching various intermediate attachments, if
the brightness adjustment knob of the B excitation
fluorescence illuminator is interfered or cannot be
operated, the intermediate attachment must be attached
again by changing its angle.
· When carrying the microscope, be sure to remove the
Bexcitation fluorescence illuminator in advance, since it
may be in danger of dropping.
Adjusting the brightness
Rotate the brightness adjustment knob b to adjust the brightness.
TIP
1
TIP
NOTE
1
a
b
3 Operation procedures

CX43-RFAB
9
2 Escaping the condenser top lens
During fluorescence observation, when the background light of the field of view becomes darker, the contrast of
the fluorescence observation image is improved. If the condenser turret is set to FL (fluorescence observation),
the background light of the field of view can be darker than that of other turret positions. If you want to make the
background light of the field of view darker effectively, escape the condenser top lens from the light path.
TIP
a
c
b
Using the finger
Insert the finger to the aperture of the stage, press the finger hook por-
tion a and move the top lens to the front (arrow direction).
Using the hook stick
If the finger cannot be inserted to the aperture of the stage
due to using the specimen holder for observing two slide
glasses or plain specimen holder, use the hook stick provided
with the B excitation fluorescence illuminator.
Insert the hook stick b in the insertion hole on the front of the stage,
hook it over the pin c near the top lens and move the top lens to
the front (arrow direction).
Be sure to store the hook stick in a safe place.
When using the plain specimen holder, it is recommended
to remove the top lens from the light path in advance prior to
observation.
1
TIP
1
NOTE
TIP

10
4 Assembly
1 Attaching the B excitation fluorescence illuminator
Be sure to set the main switch a of the B excitation
fluorescence illuminator to (OFF) before attaching or
removing the B excitation fluorescence illuminator.
Remove the cover plate for insertion portion of the fluorescence
illuminator by inserting the flathead screwdriver into the notch b.
Be sure to store the cover plate in a safe place.
Insert the B excitation fluorescence illuminator into the insertion portion
of the fluorescence illuminator with the brightness adjustment knob
c facing up, and push it completely until it touches the end.
Removing the B excitation fluorescence illuminator
Insert the allen wrench provided with the microscope frame into
the hole d at the tube attaching portion of the microscope frame,
and push the stopper e attached to the B excitation fluorescence
illuminator to pull out the B excitation fluorescence illuminator.
When removing the B excitation fluorescence illuminator,
attach the cover plate to prevent dust.
CAUTION
1
NOTE
2
1
NOTE
d
e
c
a
b

CX43-RFAB
11
2 Connecting the AC adapter and power cord
Always use the AC adapter and power cord provided by Olympus. If the proper AC adapter and the
power cord are not used, the electric safety and the EMC (Electro-Magnetic Compatibility) performance
of the product cannot be assured. If no power cord is provided, please select the proper power cord by
referring to the section “Proper selection of the power cord” at the end of this instruction manual.
CAUTION
· The power cord and AC adapter cord are vulnerable when
bent or twisted. Never subject them to excessive force.
· Be sure to set the main switch of the B excitation
fluorescence illuminator a to (OFF) before connecting
the AC adapter and the power cord.
Connect the output connector b of the AC adapter to the input con-
nector c on the rear of the microscope.
Connect the connector portion d of the power cord to the connector
e completely.
Connect the power cord’s plug f to the power outlet g on the wall.
NOTE
1
2
3
a
c
e
d
b
f
g
1
2
3

12
a
b
3 Attaching the hook stick holder
· It is recommended to attach the provided hook stick holder a
to the back of the microscope frame.
· The provided hook stick b can be fixed to the provided hook
stick holder a.
TIP

CX43-RFAB
13
5 Specifications
Item Specification
Light source Blue LED (470nm)
Rating
Body portion (rated input power): 5 V 0.85 A
AC adapter (rated input power): 100-240 V 50-60 Hz 0.4 A
AC adapter (rated output power): 5 V 2.5 A
Dimension 65 (W) x 212 (D) x 71 (H) mm
(Body portion)
Weight Approx. 0.60kg (Body portion)
Selection between
fluorescence and brightfield Selection by IN to or OUT from microscope frame
Operating
environment
· Indoor use
· Altitude: Max. 2000 meters
· Ambient temperature: 5 to 40 °C (41 to 104 °F)
· Humidity: Max. 80% (31 °C or less) (without condensation)
In case of over 31 °C (88 °F), the humidity in operating environment is decreased
linearly through 70% at 34 °C (93 °F), 60% at 37 °C (99 °F), and to 50% at 40 °C
(104°F).
· Supply voltage fluctuation: ±10 %
· Pollution degree: 2 (in accordance with IEC60664-1)
· Installation (overvoltage) category: II (in accordance with IEC60664-1)

14
Proper selection of the power supply cord
If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “Speci-
fications” and “Certified Cord” below:
Caution : In case you use a non-approved power supply cord for Olympus products, Olympus can no longer war-
rant the electrical safety of the equipment.
Specifications
Voltage rating
Current rating
Temperature rating
Length
Fittings configuration
125 V AC (for 100-120 V AC area) or, 250 V AC (for 220-240 V AC area)
6 A minimum
60 ˚C minimum
3.05 m maximum
Grounding type attachment plug cap. Opposite terminates in molded-on IEC
configuration appliance coupling.

CX43-RFAB
15
Country Agency Certification mark Country Agency Certification mark
Argentina IRAM Italy IMQ
Australia SAA Japan JET, JQA,
Austria ÖVE Netherlands KEMA
Belgium CEBEC Norway NEMKO
Canada CSA Spain AEE
Denmark DEMKO Sweden SEMKO
Table 1 Certified cord
A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with
an agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of the agencies
listed in Table 1. In case you are unable to buy locally the power supply cord which is approved by one of the agencies
mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in your
country.

16
Country Agency Certification mark Country Agency Certification mark
Finland FEI Switzerland SEV
France UTE United
Kingdom
ASTA
BSI
Germany VDE U.S.A. UL
Ireland NSAI