Olympus CKX41 User manual

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CKX41/CKX31 dimensions (unit: mm)
• OLYMPUS CORPORATION is ISO14001 certified.
• OLYMPUS CORPORATION is FM553994/ISO9001 certified.
• Illumination devices for microscope have suggested lifetimes.
Periodic inspections are required. Please visit our web site for details.
• Specifications and appearances are subject to change without any notice or obligation on the part of
the manufacturer.
CKX41/CKX31 are the environmental conscious products according to
OLYMPUS' own standards.
Main features of OLYMPUS Eco-products are as follows.
• Lead-free and arsenic-free Eco-glass for optics, such as lenses and prisms.
• Exclusion of hexavalent chrome, mercury, lead and cadmium from metal materials and surface
treatment of metal.
• Exclusion of lead solders.
• Adoption of cardboard for packing materials without styrene foam for promoting the recycling.
* A definition of exclusion depends on OLYMPUS standard. Some accessories are inapplicable.
Please visit our web site for further information: http://www.olympus.co.jp/en/eco-products/
Item CKX41 CKX31
Optical system UIS2 (Universal Infinity-corrected) optical system
Focus Vertical nosepiece movement (stage is fixed),
coaxial coarse and fine focus with tension adjustment mechanism, roller slide mechanism,
stroke: 7 mm up and 2 mm down from focus position which is 1 mm above the stage,
stroke per rotation: 36.8 mm (coarse), 0.2 mm (fine)
Revolving nosepiece Quadruple
Stage Plain stage 160 mm (L) x 250 mm (W)
Exchangeable insert plate (ø25 mm opening) incorporated ø35 petri dish holder stage
incorporated
Mechanical stage Right-hand low drive coaxial controls,
stage movement: X=120 mm, Y=78 mm, with three dish/sample holders
Substage 70 (L) X180 (W) mm
Illumination Light source 6 V, 30 W halogen lamp, lamp socket (U-LS30-3-5),
system built-in frosted and heat absorbing filters, detachable illuminator
Filter holder Insert up to 11 mm thick with ø45 mm filter, detachable
Aperture diaphragm Lever type, range: from minimum ø3 mm to maximum ø44 mm
Slider insertion With phase slider pocket and built-in slider position click stop mechanism
Condenser Detachable ultra-long working distance condenser (N.A. 0.3, W.D. 72 mm)
Contrast slider •Pre-centered phase contrast: 4X, 10X/20X/40X, empty slot
•Centerable phase contrast: 4X, 10X/20X, empty slot (40X optional, pre-centered)
•Centerable for Relief Contrast: 10X, 20X, 40X
Observation Binocular tube U-CBI30-2: inclined 30°, interpupillary distance range: 48-75 mm,
tube diopter adjustment by helicoid on left sleeve (F.N. 20)
U-BI30-2: inclined 30°, interpupillary distance range: 48-75 mm,
diopter adjustment by helicoid on left sleeve (F.N. 22)
Trinocular tube U-CTR30-2: inclined 30°, ring dovetail attachment,
interpupillary distance range: 48-75 mm, tube length
and diopter adjustment by helicoid on left sleeve
Observation optical path: 50(binocular)/50(video port) (F.N. 20)
U-TR30-2: inclined 30°, ring dovetail attachment,
interpupillary distance range: 48-75 mm, tube length
and diopter adjustment by helicoid on left sleeve
Observation optical path: 50(binocular)/50(video port) (F.N. 22)
Tilting binocular CKX-TBI: variable inclination angles from 30° to 60°,
tube interpupillary distance range: 50-76 mm,
diopter adjustment by helicoid on right sleeve (F.N. 20)
U-CTBI: variable inclination angles from 30° to 60°,
interpupillary distance range: 48-75 mm,
diopter adjustment by helicoid on right sleeve (F.N. 18)
U-TBI-3: variable inclination angles from 5° to 35°, circular
mounting dovetail attachment, interpupillary distance range:
50-76 mm, diopter adjustment by helicoid on right sleeve. (F.N. 22)
Fluorescent illuminator Detachable illuminator,
switchable slide (3-position: B excitation, G excitation, empty slot or U excitation)
FL light source 50 W Hg
FL light shutter Available
FL field stop Available
FL cubes 2 cubes (B & G), option U (cubes are not compatible with UIS2.
Filter and dichroic mirror size are same as UIS2)
Filter 1 filter
Eyepiece For U-CBI30-2/U-CTR30-2/CKX-TBI: WHB10X/WHB10X-H (F.N. 20) 10X (F.N. 20)
For U-BI30-2/U-TR30-2/U-TBI-3: WHN10x/WHN10x-H/
CROSS WHN10x (F.N. 22)
For U-CTBI: (F.N. 18)
Power supply Continuous intensity adjustment,
built-in voltage changeover switch (100/120 V, 220/240 V), frequency 50/60 Hz
CKX41/CKX31 specifications
—
—
Fixed binocular tube, inclined
45°, interpupillary distance range:
48-75 mm, diopter adjustment
by helicoid on right sleeve
Printed in Japan M1500E-0910B
Objective N.A. W.D.(mm) Remarks
PLCN4X 0.10 18.5
PLCN10X 0.25 10.6
LUCPLFLN20X 0.45 6.6-7.8
LUCPLFLN40X 0.60 2.7-4
UPLFLN4XPH 0.13 16.4 PHL (for use with IX2-SL)
CPLN10XPH 0.25 10 PHC (for use with IX2-SL)
PLCN10XPH 0.25 10.6 PH1 (for use with IX2-SL)
CPLFLN10XPH 0.3 9.5 PHC (for use with IX2-SL)
LCACHN20XPH 0.40 3.2 PHC (for use with IX2-SL)
LUCPLFLN20XPH 0.45 6.6-7.8 PH1 (for use with IX2-SL)
LCACHN40XPH 0.55 2.2 PH2 (for use with IX2-SL)
LUCPLFLN40XPH 0.6 3.0-4.2 PH2 (for use with IX2-SL)
UPLFLN4XPHP*20.13 16.4 For use with IX2-SLP
CACHN10XPHP*20.25 8.8 For use with IX2-SLP
LCACHN20XPHP*20.40 3.2 For use with IX2-SLP
LCACHN40XPHP*20.55 2.2 For use with IX2-SLP
CPLN10XRC 0.25 9.7 For use with CKX-RC
LCACHN20XRC 0.4 2.8 For use with CKX-RC
LCACHN40XRC 0.55 1.9 For use with CKX-RC
UPLFLN4X 0.13 17 U,B,G
UPLFLN10X2 0.3 10 U,B,G
UPLFLN20X 0.5 2.1 U,B,G
LUCPLFLN20X 0.45 6.6-7.8 U,B,G
LUCPLFLN40X 0.6 2.7 - 4.0 U,B,G
UIS2 objectives *1specifications
For brightfield
For phase contrast For RC For FL
*1Objective lenses are lead free.
*2Pre-centering objective
Weight: approx. 8.8 kg Rated voltage: 85 VA Weight: approx. 8 kg Rated voltage: 85 VA
Inverted Microscope
CKX41/CKX31
A New Advance in Routine Inspections

PHC type objectives minimally affected by surface tension
around the container edge
(CPLN10XPH, CPLFLN10XPH, LCACHN20XPH)
The PHC type objectives are minimally affected by surface tension
of the culture fluid, which can compromise clarity at the image
periphery. Easier multi-well observation is one of several benefits.
Combined with the
improved flatness
resulting from the use
of UIS2 optics, this
feature facilitates
clear observation of
cells even at the
edge of the container.
PHC objective
21
CKX series microscopes make cell checking quicker and easier than ever —
because they are simple to operate, require minimal optical adjustments and
capture the best possible observation images with outstanding efficiency.
Use of a tilting observation tube, which allows the user to make observations while standing,
is an especially convenient feature.
In addition, the compact design allows installation right beside the incubator,
improving safety and saving time spent on carrying specimens elsewhere for observation.
Multiple observation methods are supported, and the range of applications has been further increased.
The CKX series: designed to make routine cell checking quicker, easier and more efficient.
Phase contrast
Phase contrast
Relief contrast
Relief contrast
Fluorescence
Fluorescence
Incorporation of advanced UIS2 optics
ensures the highest level of clarity for
cell checking applications.
Clear up to the edge of the view field.
Flatness improved for 10 to 15%
Clear, wide-ranging phase contrast observation.
Ideal phase plate for cell culture.
Clear cell observation right to the edge of the container.
PHC objective virtually unaffected by surface tension.
Enhanced clarity makes it easier to
observe cell activity status
Incorporation of the world-renowned Olympus UIS2 optical system
in the CKX series makes a dramatic improvement in flatness for
10 to 15% and enables very clear, high contrast images to be
obtained up to the edge of the field view.
Adoption of a UIS2 phase plate optimized for
cell culture observation provides higher image contrast
The UIS2 phase plate adjusts the contrast according to the
thickness of the cell culture, producing higher contrast images than
those obtainable from conventional systems.
Low magnification UIS2 video adapters can be used for
0.25X, 0.35X, 0.5X and 0.63X magnifications, enabling
clear display of the complete cell on the monitor.
CKX41
A binocular tilting tube, which
allows observation while
standing, and a trinocular
observation tube which is
compatible with various video
cameras are available.
Numerous accessories make it
easy to upgrade the CKX41
according to need.
CKX31
Standard microscope for cell
culture observation with fixed
binocular tube.
The UIS2 optics provide unsurpassed optical performance
The UIS2 optical system combines with the simplicity of the light
path and the imaging excellence of a relay lenses to maintain the
highest possible image intensity and to arrive at the highest optical
correction level. Thanks to this feature, the UIS2 infinity-corrected
optical system deliver unsurpassed resolution and contrast.
In addition to improved image quality, the maximum field of view
has been extended to F.N. 22. This allows the use of various
observation tubes provided for upright microscopes (patented,
CKX41 only).
UIS2 infinity-corrected optical system
The advanced Olympus UIS2 optical system maximized the advantages of infinity
correction. Light travels through the body tube as parallel rays as it passes through
the objective. These are focused by the tube lens to form a completely aberration-
free intermediate image. Attachments can be added between the objective and the
built-in tube lens in the observation tube without any magnification factor alterations
to total magnification. Additional correction lenses are not required The UIS2
optical system delivers optimum image quality with any configuration.
Primary focus plane
Half mirror
Objective Illumination light
Reflected ray
Image forming ray
Telan lens
Parallel light
Standard field number 20
Image capturing area 22
Maximum field number 22
Standard objective

4
Just set the specimen on the stage and observe
immediately while standing, with no optical adjustments.
Pre-centered phase contrast slider for quick,
adjustment-free observation
With the pre-centered phase contrast slider, there is no need to
repeat centering each time the objective magnification is changed.
Likewise, ring slit exchange when swapping between objectives is
also unnecessary, since the ring slit is standardized for 10X, 20X
and 40X magnifications. The CKX series makes phase contrast
observations quick and easy, without optical adjustments, for much
more efficient performance of routine tasks. Since the phase plate
transmission rates are dictated by the objective in use, the
brightness remains constant
even when the magnification
is changed.
*The IX2-SL with centering mechanism
enables maximized phase contrast
performance with precise optical
adjustments.
Slim, compact design takes up minimum laboratory space
The slim, compact design minimizes the depth of the microscope,
allowing easy installation right beside the incubator or clean bench.
Specimens can be taken and immediately set on the microscope
stage for quick, efficient inspection.
Easy observation at the clean bench
The tilting binocular tube combination makes it unnecessary for
researchers to adopt uncomfortable postures like lowering their face
to the inside of the clean bench. Simply observe down the tube
while working, and raise the tube afterwards: this method is much
more convenient, since the clean bench can be opened/closed
without moving the microscope.
Tilting binocular tube for observation while standing
The binocular tube has a 30-60 degree tilting mechanism to let the
researcher make observations while standing. The specimen is
removed from the incubator and placed straight onto the
microscope stage, so that cell checking can be done immediately.
"Quick observation, quick return"— that’s the new style of routine
inspection made possible by Olympus.
Empty slot10X, 20X, 40X4X
3
10X 20X 40X

CKX41/CKX31 SYSTEM DIAGRAM
65
High-resolution relief contrast with N.A.0.5:
thick specimens like egg cells can be
observed in three dimensions
Olympus' relief contrast maintains the same shadow
direction even if the magnification is changed.
The two-stage exchange slider uses a common aperture
for 20X and 40X magnifications, so exchange between
10X/20X and 10X/40X is performed quickly.
The same feature enables easy optical adjustment:
by simply using the lever, the contrast can be
changed while centering is being performed.
Relief contrast observations
Fluorescence observations
Accessories
✬✬
✬✬✬
✬
✬
CKX31SF
Microscope set
(Eyepiece, U-LS30-3-5 lamp socket and
6 V 30 W halogen lamp included)
Eyepiece
CT-5
Centering
telescope
CKX41SF
Microscope set
(U-LS30-3-5 lamp socket and
6 V 30 W halogen lamp included)
45-LBD-IF
Day light filter
(blue ø45)
45LBD-2N
Light balancing
filter (blue ø45)
43ND25-W45
ND filter
Filter holder
(included in
CKX31SF/CKX41SF)
43IF550W45
Interference
contrast filter
(green ø45)
CKX-RCD
Relief Contrast condenser
■ Relief Contrast slider
(centerable,
ring slits included)
CK40-CPG
Glass stage
insert plate
CK40-CPG30
Glass stage
insert plate
IX2-GCP
Glass stage
insert plate
IX-CP50
Stage insert plate
(with ø50 dia.)
Stage insert
plate
Stage insert
plate type
warm plate
U-RFLT50
Power supply
unit
U-LH50HG
Lamp housing
for 50 W Hg
CKX-RFA
Fluorescence illuminator
(B and G excitation
filters included)
32ND6, 32ND12,
32ND25, 32ND50
ND filters (ø32)
CKX-NU
U excitation filter
Terasaki holder
(included in CK40-MVR)
ø35 diameter
petri dish holder
(included in CK40-MVR)
ON3 manipulator
CK40-MVR
Attachable
mechanical stage
CK2-SS
Stage extension
plate
Stage-mounting
type adapter
Slide glass holder
(included in CK40-MVR)
U-BI30-2
Binocular tube U-TBI-3
Tilting
binocular tube
CKX-TBI
Tilting binocular tube
(eyepieces incorporated
F.N. 20)
U-CBI30-2
Binocular tube U-CTBI Ergonomical
binocular tube
(eyepieces incorporated
F.N. 18)
U-TR30-2
Trinocular tube
U-CTR30-2
Trinocular tube
U-FMT
F mount
adapter U-SMAD
Sony
mount
adapter
U-BMAD
Bayonet
mount
adapter
U-CMAD3
C mount
adapter
U-TMAD
T mount
adapter
U-TV1X-2
Direct image
camera port
U-TV0.25XC
C mount camera
port with 0.25x lens
U-TV0.35XC-2
C mount
camera port
with 0.35x lens
U-TV0.5XC-3
C mount
camera port
with0.5x lens
U-TV0.63XC
C mount
camera port
with0.63x lens
CAMERA
SYSTEM
● IX2-SL
Phase contrast slider
(centerable,
PHL ring slit included)
IX2-SLPHC/
IX2-SLPH1/
IX2-SLPH2
Phase contrast ring slit
▲ IX2-SLP
Phase contrast slider
(pre-centered)
CT-5
Centering
telescope
WHB10X
WHB10X-H
Eyepiece
U-CT30
Centering
telescope
WHN10X
WHN10X-H
CROSS WHN10X
Eyepieces
PLCN4X
PLCN10X
LUCPLFLN20X
LUCPLFLN40X
Objectives
●
UPLFLN4XPH
CPLN10XPH
PLCN10XPH
CPLFLN10XPH
LCACHN20XPH
LUCPLFLN20XPH
LCACHN40XPH
LUCPLFLN40XPH
Objectives
(for use with IX2-SL)
▲
UPLFLN4XPHP
CACHN10XPHP
LCACHN20XPHP
LCACHN40XPHP
Objectives
(for use with IX2-SLP)
■
CPLN10XRC
LCACHN20XRC
LCACHN40XRC
Objectives
(for use with CKX-RC)
IX2-BCTP
Hemacytometer holder
Suitable for hemacytometer holder and
other kinds of micro-plate
The mechanical stage offers excellent
inspection performance with hemacytometer
holders or other micro-plates.
Glass stage insert plate and warm plate
Also included are a glass stage insert plate,
for simple confirmation of the objective in
use, and a warm plate for easy temperature
control (CKX41 only).
Vertical installation avoids interference with
manipulators
Vertical installation helps the user to avoid accidentally
touching the manipulators while operating the slider or
making optical adjustments.
Fluorescence observation system with
tree-position allows the use of U excitation.
Adopted renowned UIS2 optics, this system provides
B and G excitations and optionally U excitation as well.
All three can be provided simultaneously, with quick, easy
exchange among them. Many different UIS2 filters can also
be installed, according to requirements, enabling this
system to meet a wide range of fluorescence
observations needs. Also, thanks to newly developed
fluorescent illuminator, which secured UV transmission
rate, and a variety of UIS2 fluorescent objectives, clear
and high contrast
fluorescent images
are provided.