Olympus SLIDEVIEW VS200 User manual

Instructions for use
SLIDEVIEW
VS200
Optical Microscope and Accessory

Any copyrights relating to this manual shall belong to
OlympusSoftImagingSolutionsGmbH.
We at Olympus Soft Imaging Solutions GmbH have tried to make the information contained
in this manual as accurate and reliable as possible. Nevertheless, Olympus Soft Imaging
Solutions GmbH disclaims any warranty of any kind, whether expressed or implied, as to any
matter whatsoever relating to this manual, including without limitation the merchantability
or fitness for any particular purpose. Olympus Soft Imaging Solutions GmbH will from time to
time revise the software described in this manual and reserves the right to make such
changes without obligation to notify the purchaser. In no event shall Olympus Soft Imaging
Solutions GmbH be liable for any indirect, special, incidental, or consequential damages
arising out of purchase or use of this manual or the information contained herein.
No part of this document may be reproduced or transmitted in any form or by any means,
electronic or mechanical for any purpose, without the prior written permission of
OlympusSoftImagingSolutionsGmbH.
All brands are Trademark or registered Trademark of their respective owners.
© Olympus Soft Imaging Solutions GmbH
All rights reserved
Version 510_InstructionsForUse_VS200_de_00_xxJuly2019
Original instructions
Manufactured by
OLYMPUS SOFT IMAGING SOLUTIONS GmbH
Johann-Krane-Weg 39
48149 Münster, Germany
Phone: +49 251 - 798 00 0
Fax: +49 251 - 798 00 6060
Email: [e-mail]

Contents
1 About this manual 7
1.1 Notes on qualifications 7
1.2 Intended use 7
1.3 Other applicable documents 8
1.4 Symbols in this documentation 8
2 Safety 9
2.1 Categories and symbols in the safety instructions 9
2.2 Caution labels 9
2.3 Caution labels on the product 9
2.4 General safety instructions 10
2.5 Safety instructions for the laser 13
2.6 Safety instructions for X-Cite Turbo and X-Cite Xylis 13
3 Hinweise zum Aufstellen 14
3.1 Betriebs- und Lagerbedingungen 14
4 Tools and accessories 16
5 Scope of supply 17
6 System diagram 20
6.1 Available fluorescence components 20
7 Trays 22
7.1 Tray description 22
7.2 VS200 Tray types 22
8 Specifications 24
9 Unboxing 26
9.1 VS200 Scanner 26
9.1.1 Mounting of handles - VS200 scanner 26
9.2 Transportation locks for the VS200 scanner 26
9.2.1 Removing transportation lock from stage 27
9.2.2 Removing transportation lock from transmitted light condenser
(BX3-UCD8A) 28
9.3 VS200 Loader 30
9.3.1 VS200 Trays 32
9.3.2 PC and monitor 32
9.4 VS200 loader transportation locks 32
9.4.1 Removing transportation lock from VS200 loader’s tray hotel 33
9.4.2 Removing transportation lock from SCARA robot arm 34
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9.4.3 Removing transportation lock from SCARA robot arm’s coun-
terweight 35
10 Mounting of components 37
10.1 Scanner 37
10.1.1 Objectives 37
10.1.2 Immersion Objectives 38
10.1.3 Phase Contrast (PH) objectives 39
11 Mounting fluorescence components 40
11.1 IX3-RFACA and IX3-RFALFE 40
11.2 Fluorescence filter wheels or camera adapter for monochrome camera 45
11.2.1 U-FFWR (Motorized fast reflected light filter wheel) 45
11.2.2 Add or replace filter (U-FFWR) 47
11.2.3 U-FFWO T3 (Motorized fast observation filter wheel) 49
11.2.4 Add or replace filter (U-FFWO) 51
11.2.5 TV 1.0x adapter 52
11.3 Monochrome camera 52
11.3.1 Orientation of the monochrome camera 53
11.4 X-Cite adapter 56
11.5 4x Objective 57
11.6 LED light source 57
11.7 Filter set 58
11.7.1 U-FF filter cube (IX3) 58
11.7.2 Add or replace filter cube (IX3) 59
11.7.3 U-FDICT filter cube 60
11.7.4 X-Cite Turbo 61
12 Assembly of the housing for scanner 62
12.1 Left panel 62
12.2 Right Panel 64
12.3 Back panel 65
12.4 Top panel 66
13 Cabling 70
14 Assembly of the loader housing 72
14.1 Top panel 72
14.2 Back panel 72
14.3 Front panels 73
15 Verbindung VS200 Scanner und VS200 Loader 75
15.1 Mechanical Connection VS200 scanner and VS200 loader 75
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16 PC operating system language 77
17 VS200 ASW software setup 78
18 VS200 device configuration 85
18.1 Activate the motorized polarizer 86
18.2 Activate the VS200 liquid dispenser 86
18.3 Device settings - objectives 87
18.4 Device settings - filter 88
18.5 Manual device configuration 89
18.5.1 ORCA monochrome camera 90
18.6 Device customization 90
18.6.1 ORCA camera adjustments 91
18.6.2 Setup phase contrast (PH) observation method 92
18.6.3 Setup polarization (Pol) observation method 94
18.6.4 Create or adjust an observation method 95
18.7 VS200 LED lamp voltages 97
18.7.1 Orca Flash 4.0 voltages (%) 97
18.7.2 Orca Fusion voltages (%) 97
18.7.3 VS-304M voltages (%) 98
18.7.4 VS-264C voltages (%) 98
19 How to insert a slide into a tray 99
19.1 How to insert a tray into the VS200 scanner 99
19.2 Insert a tray into the VS200 loader 100
20 Calibrate VS200 using the Olympus Calibration Slide 101
20.1 Stage limits - Z Axis 103
20.2 Camera adapter calibration for iDS (VS-264C) camera 106
20.3 Camera-To-Stage Rotation 110
20.4 XY Objective Shift / Parfocality 112
20.5 Shading Correction (Brightfield) 115
20.6 Shading correction of the label area 123
20.6.1 Preparation of calibration slide 123
20.6.2 Shading correction for polarization (Pol) 131
20.7 Magnification Test Scan 136
21 Additional calibrations for a fluorescence system 138
21.1 Camera adapter 139
21.1.1 Camera adapter U-FFWO T3 139
21.2 Camera-To-Stage Rotation 144
21.3 Camera shift 146
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21.4 Lens Correction 150
21.5 Shading correction BFMono 153
21.6 Magnification Test Scan 158
21.7 Shading correction for fluorescence observation methods 160
21.8 Shading correction for darkfield (DFMono) 164
21.9 Shading correction for PhaseContrast (PH) 167
21.10 Channel-XY-Shift 171
22 Cleaning the system 174
22.1 Cleaning the scanner 174
22.2 Cleaning the loader 174
22.3 Cleaning the trays 175
22.4 Cleaning the X-Cite Turbo 175
22.5 Cleaning the immersion objective 175
23 Additional software installation 178
23.1 OlyVIA 178
23.2 VS200 ASW Desktop 178
23.3 NetImage Server SQL (NIS-SQL) and Webinterface 178
24 Troubleshooting 179
24.1 Hardware not available 179
24.2 "No camera" error 179
24.3 Tray not active 179
24.4 Image too bright 180
24.5 Setting the Koehler illumination 180
25 Proper selection of the power supply cord 186
26 Declarations of conformity and disposal 189
26.1 CE Conformity (Europe) 189
26.2 WEEE declaration (Europe) 189
26.3 RoHS Conformity (Europe) 189
26.4 FCC conformity (USA) 189
26.5 For Korea only 190
26.6 China RoHS conformity (China) 190
26.7 RFID 190
26.7.1 RFID (Korea) 190
26.7.2 RFID (USA) 190
26.7.3 RFID (Canada) 191
26.7.4 RFID (Taiwan) 191
27 Support 192
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1 About this manual
1 About this manual
This manual for the VS200 system is for Olympus technicians as well as users of the
VS200 system who have been trained by Olympus. The manual contains important
information on how to operate the VS200 system safely and correctly.
In addition, this manual describes how to unbox and install the system as well as
how to install further components. These tasks must expressly only be performed by
Olympus. As a user you are not allowed to unbox or install the VS200 system by your-
self!
To ensure the safety, obtain optimum performance and to familiarize yourself fully
with the use of this product, study this manual thoroughly before installing this
product, and always keep this manual at hand when operating this product.
Retain this instruction manual in an easily accessible place near the work area for
future reference.
Installing and configuring a liquid dispenser, and upgrading to a VS200
loader are not in the scope of this document. These must only be installed
and adjusted by technical service of Olympus.
1.1 Notes on qualifications
Olympus personnel Instructions for tasks that must only be performed by Olympus
are identified with the following note:
The units described below must be assembled and adjus-
ted by Olympus. If these units are assembled or adjusted
by the customer, the operations are not ensured.
1.2 Intended use
The system is intended to be used for the acquisition of virtual slide images. The sys-
tem is intended to be used for research only.
If the VS200 system is used in a manner not specified by this manual, the safety of
the user may be at risk. In addition, the VS200 system may also be damaged. Always
use the VS200 system according to this instruction manual.
Olympus Soft Imaging Solutions GmbH takes no responsibility for damaged caused
by unintended use.
Foreseeable misuse
The system is not intended to be used for primary diagnosis.
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1.3 Other applicable documents
Familiarize yourself with all of the other manuals for the components of the system.
Take special note of the safety instructions they contain.
1.4 Symbols in this documentation
Tools required for performing the steps
Preconditions for the subsequent steps
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1 About this manual
OLYMPUS VS200 System

2 Safety
2 Safety
If the product is used in a manner not specified by this manual, the safety of the user
may be imperiled. In addition, the product may also be damaged. Always use the
product as outlined in this instruction manual.
2.1 Categories and symbols in the safety instructions
The safety instructions in this manual use symbols and keywords that are divided
into the following categories:
CAUTION
This warning sign and the word CAUTION indicate dangerous situations that
can lead to minor injury if ignored.
ATTENTION
The exclamation mark and the word ATTENTION indicate situations where irre-
parable damage to the product can occur if ignored.
Useful tips and important information
This symbol indicates useful notes, tips and important information.
2.2 Caution labels
Indicates a non-specific general hazard.
This warning sign indicates that there is a crush hazard.
2.3 Caution labels on the product
Caution labels have been placed at positions where special care is required when
handling and operating the system. Always pay attention to the caution labels.
CAUTION
Moving components can cause injury
Im Inneren des VS200 Systems verfahren Komponenten. Gaps open and close.
Hands and fingers can get crushed.
Sobald das System mit der Stromversorgung verbunden ist, Haare, Finger,
Hände und andere Körperteile sowie Kleidung und Gegenstände aus dem
Inneren des VS200 Systems fernhalten.
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Position of caution labels
U-FFWO
Loader
Im Bereich des Mikroskoptisches
Nosepiece
Flap door
Flap door motor
2.4 General safety instructions
Do not plug anything into or unplug anything from the ports when the system is
connected to the power supply.
The system is not intended to be used for general microscopy tasks.
No guarantee is provided on an image file. For example, even if the following
state is generated attributable to an operation error of an observer, malfunction
of the device, a system failure including a potential one, or the like, or at the time
of normal operation or normal movement, it will not be possible to perform
image restoration or damage compensation.
1. Abnormal state of an image
2. Abnormal state of file property such as a file name and file date and time
3. Disappearance of an image
Confirm, edit or save the image for backup through care of the observer. To be
noted is that the electronic recording media such as USB memory, HDD and
DVD-R have retention lives in general and there may be the case that a file saved
may disappear in several years.
Further, there is no authenticity or tamper-proof characteristic.
Prevent from strong vibrations as they can impair the image quality.
Regarding to 3-axes control unit for scanning stages and motorised focus drives,
1. Item caution for use
2. Installation prevent from strong vibrations and risk of explosion
3. Temperature prevent from direct sunlight or strong heat sources
4. Humidity prevent from splash water and moisture
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2 Safety
OLYMPUS VS200 System