Olympus CH30 User manual
OLYMPUS
INSTRUCTIONS
CH30/CH40
BIOLOGICAL
MICROSCOPE
IMPORTANT
Safety
Symbols
The following
symbols
are
found
on the
microscope.
Study
the
meaning
of
the
symbols,
and
always
use
the
microscope
in
the
safest
possible
manner.
1.
Make
sur
that
the
voltage
selector
switch
@
located
on
the
underside
of
the
base
of
the
microscope
frame
is
set
to
conform
with
the
local
‘mains
voltage.
Move
the
switch
using
an
Allen
wrench,
etc.
(Fig.
1)
©
When
shipped
from
the
factory,
the
switch
is
set
to
110-120V
and
230-240V
in
100V
and
2009
areas,
respectively.
2.
To
avoid
potential
shock
hazard
and
fire,
always
switch
the
main
switch
©
to
"O"
(OFF) and
disconnect
the
power
cord
from
the
wall
outlet
before
replacing
the
bulb.
Allow
the
lamp
housing
cover
@and
the
bull
to
cool
before
touching,
3.
Install
the
microscope
on
a
level
table.
The
air
vents
on
the
underside
of
the
base
should
never
be
blocked
such
as
by
placing
the
microscope
on
a
flexible
surface,
e.g.,
a
carpet,
etc,
as
this
could
result
in
overheating
and
cause
a
fire,
4.
Always
use
the
power
cord
provided
by
Olympus.
If
no
power
cord
is
provided,
please
select
the
proper
power
cord
by
referring
to
the
section
“PROPER
SELECTION
OF
THE
POWER
SUPPLY
CORD”
ar
the
end
of
this
instruction
manual.
If
the
proper
power
cord
is
not
used,
Olympus
can
no
longer
warrant
the
electrical
safety
and
performance
of
the
equipment
5.
Connect
the
power
cord
correctly
and
ensure
that
the
ground
terminal
of
the
power
supply
and
that
of
the
wall
outlet
are
properly
connected
H
the
equipment
is
not
grounded,
Olympus
can
no
longer
warrant
the
electrical
safety
and
performance
of
the
equipment.
6.
Never
insert
metal
objects,
etc.
into
the
air
vents
of
the
microscope
frame
as
this
will
result
in
electrical
shock
and/or
personal
injury.
Symbol
Explanation
Indicates
that
the
surface
becomes
hot,
and
should
not
be
touched
with bare
hands,
Before
use,
carefully
read
the
instruction
manual.
Improper
handling
could
result
in
injury
to
the
user
and/or
damage
to
the
equipment.
Indicates
that
the
main
switch
is
ON,
A
A
|
o
Indicates
that
the
main
switch
is
OFF.
€
Getting
Ready
1.
A
microscope
is
a
precision
instrument.
Handle
it
with care
‘and
avoid
subjecting
it
to
sudden
or
severe
impact.
2.
Do
not
use
the
microscope
where
it
is
subjected
to
direct
sunlight, high
temperature
and
humidity,
dust
or
vibrations.
(For
operating
environment
conditions,
refer
to
and
adhere
to
the
conditions
specified
in
Section
6,
SPECIFICATIONS
on
page
16)
3.
The
tension
ofthe
coarse
focus
adjustment
knob
should
only
be
adjusted
by
means
of
the
tension
adjustment
ring,
4.
Heat
from
this
microscope
is
lod
away
by
natural
convection,
Consequenti,
do
not
use
itina
constricted
space
and
ensure
that
the
room
is
well
ventilated,
5.
When
moving
the
microscope,
carry
it
with
one
hand
under
the
base
D
and
the
other
hand
holding
at
the
recessed
handle
‘on
the
rear
of
the
arm
@
as
shown
in
the
illustration
on
the
left.
Handle
the
microscope
with care
when
moving
it
*
When
moving
the
microscope,
never
hold
the
stage
or
binocular
section
of
the
observation
tube,
etc.
Also
make
sure
that
eyepieces,
specimen,
etc.
do
not
falloff.
*
Sliding
the
microscope
on the
surface
of
the
table
may
Faz
damage
or
tear
off
the
rubber
feet
and/or
scratch
the
table
top
surface.
Maintenance
and
Storage
1.
Clean
all
glass
components
by
wiping
gently
with
gauze.
To
remove
fingerprints
or
oil
smudges,
wipe
with
gauze
slightly
moistened
with
a
mixture
of
ether
(70%)
and alcohol
(80%)
*
Do
not
use
a
mixture
of
ether
(70%)
and
alcohol
(30%)
to
clean
the
lower
lens
(made
of
optical
plastic)
of
the
eyepiece
(NCWHK10X)
because
such
solvents
cloud
the
lens.
If
dust
adheres
to
the lens,
blow
it
off
or
wipe
it
away
gently
with
a
dry
cloth.
‘Since
solvents
such
as
ether and
alcohol
are
highly
flammable,
they
must
be
handled
carefully.
Be
sure
to
keep
these
chemicals
away
from open flames
or
potential
sources
of
electrical
sparks
—
for
example,
electrical
equipment
that
is
being
switched
on
or
off,
Also
remember
to
always
use
these
chemicals
only
in
@
well-ventilated
room.
2.
Do
not
attempt
to
use
organic
solvents
to
clean
the
non-optical
components
of
the
microscope.
To
clean
these,
use
a
lint-free,
soft
cloth
lightly
moistened
with
a
diluted neutral
detergent.
2.
Do
not
disassemble
any
part
of
the
microscope
as
malfunction
or
damage
may
occur
4,
When
not
using
the
microscope,
keep
it
covered
with
the
provided
dust
cover.
Warning
Labels
Warning
labels
are
affixed
at
parts
where
special
precaution
is
required
when
handling
and
using
the
microscope,
Always
heed
the
warnings,
[Caution
for
Warning
Label
Position
|
Base
Underside
"gay
po
car
If
warning
labels
become
soiled,
peel
off,
etc.
contact
your
local
Olympus
representative
to
have
them
replaced.
€
cu
I
the
microscope
is
operated
in
a
manner
not
specified
by
this
manual,
the
safety
of
the
user
may
be
imperiled.
In
‘addition,
the
equipment
may
also
be
damaged.
Always
operate
the
equipment
as
outlined
in
this
instruction
manual
CONTENTS
21
Assembly
Diagram
22
Detailed
Assembly
Procedure.
5-1
Base..
52 Stage
53
Observation
Tube
5-4
Condenser
55
Focusing
Adjustment
Knobs
5-6
Immersion
Objectives
.
57
Photomicrography
5-8
Phase
Contrast
Attachments
13
13
16
E
PROPER
SELECTION
OF
THE
POWER
SUPPLY
CORD...
2
CH30/CH40
The
diagram
below
shows
how
to
assemble
the
various
components,
The
numbers
indicate
the
order
of
assembly.
‘#
When
assembling
the
microscope,
make
sure
that
all
parts
are free
of
dust
and
dirt,
and
avoid
scratching
any
parts
or
touching
glass
surfaces.
+
Retain
the
provided
Allen
wrench.
It
should
be
used
when
units
are
replaced
or
added.
2,
Cord
rest
[optional
for
CH30/CH40)
Fiold irs
digphvagm
attachment
(optional
for
CH30)
{optional
for
CH30)
Filter
holder
(for
CH30)
1
6V,
20W
halogen
bulb
Kor
CH30)
6V,
30W
halogen
bull
tor
chao)
CH30/CH40
Я
Mounting
the
Bulb
(Replacement)
Fo.)
1.
Tum
the
microscope
frame
on
its
side
and
pull
the
lamp
housing
knob
on
the
underside
of
the
base
to
open
the
lamp
housing
cover.
2,
Holding
the
halogen
bulb
@
contained
in
the
polyethylene
bag
to
avoid
leaving
fingerprints
on
the
bulb, fully
insert
the
contact
pins
into
the
bulb
socket
@.
When
properly
seated,
pul
off
the
polyethylene
bag,
Applicable
Bulb
))
(CH30:
6V,
20W
Halogen
bulb:
GVZOW
HAL
(Philips
7388)
040:
ϐν,
30W
Halogen
bulb:
6V30W
HAL
(Philips
5761)
À
Always
use
the
designated
bulb.
Use
of
improper
bulb
may
result
in
fire,
À
Do
not
touch
the
bulb
with
bare
hands.
If
fingerprints
are
‘accidentally
left
on the
bulb,
wipe
the
bulb with
a
sof,
lint-free
cloth
moistened
with
alcohol.
Using
a
contaminated
bulb
will
shorten
the
service
life
of
the
bulb.
3.
With
the
lamp
housing
knob
stil
pulled
out,
close
the
lamp
housing
cover.
Then
push
in
the
lamp
housing
knob
to
lock
the
cover
+
The
lamp
housing
cover
cannot
be
closed
if
the
knob
is
pushed
in
before
you
attempt
to
close
cover.
<
Proc
ns
for
Bulb
Replacement
‘A
Whenever
you
replace
the
bulb
during
use
or
right
after
use,
first
move
the
main
switch
to
“O"
(OFF),
disconnect
the
power
cord
from
the
wall
outlet,
and
allow
the
bulb
and
parts
around
the
bulb
to
cool
before
touching.
#
If
the
bulb
burns
out
during
an
observation
and
needs
to
be
replaced,
remove
eyapieces,
specimen,
and
other
objects
likely
to
fall
off,
before
tilting
the
microscope
framo
to
replace
the
bulb.
Mounting
the
Observation
Tube
(Fig.
2)
1.
Using
the
provided
Allen
wrench,
loosen
the
observation
tube
clamping
screw
©,
2.
Insert
the
circular
dovetail
mount
at
the
bottom
of
the
observation
|
tube
into
the
opening
on
the
microscope
frame,
positioning
the
‘observation
tube
to
point
the
binocular
eyopieces towards
the
front
‘Clamp
the
observation
tube
by
tightening
the
clamping
screw
©.
Fig.2
№
Fig.
3
Mounting
the
Eyepieces
(Fig.
3,4)
‘Binocular
Tube
(CH3-B145),
Monocular
Tube
(CH3-MO45)
1,
Remove
the
eyepiece
dust
cap
©
(Fig.
3)
2.
Insert
the
eyepiece
@
into
the
eyepiece
sleeve
as
far
as
it
wil
go.
ig.
3)
'3,
Mount
the
other
eyepiece
in
the
same
manner.
Eyepiece
micrometer
discs
can
be
inserted
into
the
NCWHK10X
eyepieces.
Use
10
mm/100
micrometer
discs
1,
Unscrew
the
micrometer
mounting
frame
@
from
the
eyepiece
by
turning
counterclockwise.
2,
Place
a
micrometer
disc into
the
frame,
The
engraving
on
the
micrometer
disc
should
face
downward
into
the
micrometer
‘mounting
frame
@.
(Fig.
4)
3.
Screw
the
micrometer
mounting
frame
back
into
the
eyepiece
tube.
(Fig.
4)
+
Use
19
mm
diameter
micrometer
discs.
r -
[
Using
Eyepiece
Mierometer
Dises
(optional)
|-
Я
Mounting
the
Objectives
(Fig.
8)
Mount
the
objectives
on
the
revalving
nosepiece
in
such
a
manner
that
the
magnification
increases
from
low
to
higher
powers
in
a
counterclockwise
direction.
Oi
there
are
empty
sockets
with
no
objective
mounted,
make
sure
to
attach
the
provided
dust
plugs.
I
Mounting
the
Condenser
(Fig.
6)
1.
Tur
the
coarse
acustment
knob
D
to
ris
the
stage
tots
highest
postion
2.
Tum
the
condenser
height
adjustment
knob
@
to
lower
the
condenser
holder
to
the
position
where
the
clamping
screw
@
can
be
turned
3.
Insert
the
condenser
@
into
the
mounting
hole
©
and
clamp
by
tightening
the
clamping
screw
@.
Positioning
the
condenser
with
the
diaphragm
lever
@
at
the
front
facilitates
operation
4,
Turn
the
condenser
height
adjustment
knob
@
to
raise
the
condenser
holder
to
its
highest
position.
cae
CH30/CH40
Я
Mounting
the
Biue
Fitter
(ion
©
The
blue
filter
converts
the
color
temperature
of
tungsten
illumination
into
the
color
temperature of
daylight
to
show
the
specimen
in
natural
colors,
Place
the
32.5
mm
diameter
blue
filter
@
in
the
fier
holder
@ or
the
compensating
lens,
ig
ier
Hr
e
Compri
Lan
ta)
1,
Insert
the
filter holder
or
the
compensating
lens
@ into
the
condenser
from
below
until
it
seats
with
cick.
2,
When
mounting
the
compensating
lens,
place
the
lens
so
that
the
‘centering
screws © are
at
the
front,
Mounting
the
Field
Iris
Diaphragm
Attachment
(CH30
only)
(Fig.
9)
Holding
the
field
iris
diaphragm
attachment @
over
the
light
glass
exit
(O
with
the
filter
holder
notches
@
positioned
as
shown
in
Fig.
9,
insert
the
attachment
into
the
light
glass
exit
opening
untilit
seats
wath
a
click
+
Attempting
to
insert
the
attachment
with
the
notches
@
facing
in
other
directions
than
shown
in
Fig.
9
may
break
the
mount-
ing
clip.
©
Connecting
the
Power
Cord
(Figs.
101,12)
À
Cables
and
cords
are
vulnerable
when
bent
or
twisted.
Never
‘subject
them
to
excessive
force.
A
Make
sure
that the
main
switch
©
is
on
"O"
(OFF)
before
‘connecting
the
power
cord.
(Fig.
10)
A
Always
use
the
power
cord
provided
by
Olympus.
If
no
power
cord
is
provided,
please
select
the
proper
power
‘cord
by
referring
to the
section
“PROPER SELECTION
OF
THE
POWER
SUPPLY
CORD”
at
the
end
of
this
instruction
manual.
es
B
1.
Connect
the
power
cord
plug
@
to
the
AC
receptacle
®.
(Fig.
11)
¿
é
2.
Plug
the
power
cord
plug
@
into
a
wall
outlet.
(Fig.
12)
‘A
Connect
the
power
cord
correctly
and
ensure
that
the
ground
terminal
of
the
power
supply
and
that
of
the
wall
outlet
are
properly
connected.
the
equipment
is
net
grounded,
Olympus
can
no
longer
war-
rant
the
electrical
safety
and
performance
of
the
equipment.
€
Cord
Rest
(CH3-cH)
(optional)
(Figs.
13,14)
(Q
When
the
cord
røst
(CH3-CH)
is
attached
on the
rear
panel
of
the
microscope
frame,
the
power
cord
can
be
wound
around
it
for
storage.
Align
the
hooks
@
on
the
cord
rest
O
with the
air
vent
sits
at
©,
‘and
insert
the
hooks
into
the
sits
until
the
cord
rest
is
pressed
against
the
rear
panel
ofthe
microscope
frame.
Then
pull
the
cord
rest
down
to
secure
it.
Note:
The
illustration
of
the
cord
rest
only
shows
the
sections
relevant
for
the
explanation
here.
The
actual
cord
rest
looks
somewhat
different.
+
Do
not
grasp
the
cord
rest
when
moving
the
microscope.
The
cord
rest
may
suddenly
come
loose
and
you
may
drop
the
microscope
causing
material
damage
or
personal
injury.
Detaching—Move
the
microscope
frame
to
the
edge
of
the
table
©.
Then
apply
@
screwdriver
@
or
Allan
wrench
to
the
lower
portion
of
the
cord
rest
©)
and
push
in
the [1]
directions
to
move
the
entire
cord
rest
upward
to
allow
it
to
be
detached.
Fig.
14
이
commas
CH30/CH40
“Field
iris
diaphragm
dial
RVATION
PROCEDUR
Switch
the
main
switch
to
"I"
(ON)
and
adjust
the
brightness
with
the
light
intensity
knob.
(Page
10)
“Tum
the
revolving
nosepiece
to
engage
the
10X
objective.
Make
sure
that
the
revolving
nosepiece
stops
with
an
audible
click
Place
a
specimen
on the
stage.
(Page
10)
Turn
the
X-axis
knob
© and
Y-axis
knab
@
to
move
the
specimen
into
the
light
path.
(Page
11)
Looking
through
the
right
eyepiece
with
your
right
eye,
turn
the
coarse
adjustment
knob to
bring
the
specimen
into
focus.
After
obtaining
approximate
focus,
use
the
fine
adjustment
knob
to
make
fine
adjustments.
Looking
through
the
left
eyepiece
with
your
left
eye,
turn
the
diopter
adjustment
ring
to
focus
the
specimen.
(Page
11)
CH30/CH40
7.
Adjust
the
interpupillay
distance
of
the
eyepleces.
(Page
11)
8.
Center
the
field
iris
diaphragm
by
manipulating
the
two
centering
screws,
on the
compensating
lens.
(CH40
only)
(Page
12)
In
case
of
CH30,
centration
is
not
necessary.
If
the
optional
field
i
diaphragm
attachment
is
mounted,
center
the
attachment
in
the
same
manner
as
described
above.
9.
Engage
the
objective
to
be
used
for
observation
and
adjust
the
light
n-
tensity
to
the
desired
level,
then
readjust
the
focus.
10.
Adjust
the
field
iris
diaphragm.
This
only
applies
to
the
CH30
if
the
optional
field
iris
diaphragm
attach-
ment
is
mounted.
S3HNG300H4
NOLLVAHASEO
40
AHVAINNS
11.
Adjust
the
aperture
iris
diaphragm.
(Page
12)
rr
5
10
A
turning
on
the
Bulb
(Fig, 15)
1.
Switch
the
main
switch
@
to
“1”
(ON)
2.
Turning
the
light
intensity
knob
@
clockwise
in
the
direction
of
higher
numbers
makes
the
illumination
brighter,
and
vice
versa,
Field
Iris
Diaphragm
(CH40
only)
(Fig.
18)
Using
the
field
iris
diaphragm
dial
©,
adjust
the
diameter
of
the
field
its for
objective
power
to
the
extent
that
it
just
cireumscribes
the
field
of
view.
When
the
field
iris
diaphragm
is
stopped
down
to
circum-
scribe
the
field
of
view,
it
will
exclude
extraneous
light
and
improve
image
contrast
within
the
field
of
view.
+
When
using
100X
objective,
the
field
iris
diaphragm
image
will
not
be
visible
within
the
field
of
view.
Accordingly,
stop
down
the
diaphragm
to
its
smallest
diameter.
On
case
of
CH30,
the
field ins
diaphragm
attachment
is
mounted
on
the
light
exit
glass
of
the
microscope
base.
©
Specimen
Placement
(Fiom
+
Releasing
the
curved
finger
with
great
force,
or
suddenly
re-
leasing
your
grip
on
the
curved
finger
knob
while
releasing
the
curved
finger,
will
crack
or
damage
the
slide
glass.
Always
release
the
curved
finger
with
great
car
‘Specimen
Holder
for
Single
Slides
1.
Tüm
the
coarse
adjustment
knob
@
counterclockwise
in
the
direc-
tion
of
the
arrow
to
lower
the
stage.
2.
Open
the
springoaded
curved
finger
@
on
the
specimen
holder
and
slide
the
specimen
slide
into
the
specimen
holder
from
the
front.
3.
After
placing
the
slide
as
far
as
it
will
go,
gently
release
the
curved
finger
O.
‘Specimen
Holder
for
2
Specimen
Slides
1.
Place
the
first
specimen
slide
as
described
in
steps
1
and
2
above.
Then
place
the
second
specimen
slide so
that
it
contacts
the
first
specimen
slide,
2.
Gently
release
the
curved
finger
©.
CH30/CH40
Placing
the
Specimen
Slide
with
One
Hand
Place
the
specimen
slide
at
the
front
of
the
stage,
then
slide the
specimen
slide
on
the
stage
surface
to
slowly
and
gradually
open
the
curved
finger
in
the
direction
of
the
arrow.
Insert the
specimen
slide
into
the
specimen
holder
Until
itis
fully
and
properly
seated
in
the
specimen
holder.
Use
cover
glasses
of
0.17
mm
thickness
in
combination
with
objectives
marked
with
the
“160/0.17" engraving
for
optimum
performance
of
these
objectives.
‘Specimen
slides
with
a
thickness
between
0.9
mm
and
1.2
mm
are
recommended
for
CH30/CH40
microscopes
Using
thicker
specimen
sides
may prevent
correct
formation
of
the
field
iris
diaphragm
image
on the
specimen
surface
and
illumination
will
not
be
correct
in
case
of
darkfield
observation
Fig.
20
Moving
the
Specimen
(Fig.
18)
Turning
the
Y-axis
knob
@
moves
the
specimen
in
the
Y-axis
direc-
tion.
Turing
the
X-axis
knob
@
moves
the
specimen
in
the
X-axis
direction.
*
Never
move
the
specimen
by
holding
the
specimen holder
or
‘stage
directly
as
this
will
damage
the
knob
rotation
mechanism.
k
When
further
movement
of
the
stage
or
specimen
holder
is
prevented
by
the
provided
limiting
mechanism,
the
tension
of
the
Y-axis
and
X-axis
knob
rotation
will
increaso.
At
this
point,
iting
mechanism.
©
Adiusting
the
interpupitiary
Distance
(Fig.
19)
While
looking
through
the
eyepieces,
adjust
for
binocular
vision
until
the
left
and
right
fields
of
view
coincide
completely.
Adjust
so
that
the
two
index
dots
+
are
horizontal.
‘©
Placing
the
interpupilary
distance
indexes
so
that
they
are
parallel
with
the
horizontal
lines
faciitates
the
adjustment
Also
keep
the
dots
parallel
with
the
horizontal
lines
in
case
of
interpupilary
distances
other
than
60, 60, 70,
and
76.
©
Nate
your
interpupilary
distance
so
that
it
can
be
quickly
duplicated
Diopter
Adjustment
(Fig.
20)
1.
Looking
through
the
right
eyepiece
with
your
right
eye,
rotate
the
coarse
and
fine
adjustment
knobs
to
bring
the
specimen
into
focus.
2.
Looking
through
the
left
eyepiece
with your
left
eye,
turn
the
diopter
adjustment
ring
©
to
focus
the
specimen.
11
12
Fig.
21
ση
apn
image
Eyepiece
FE
Se
Objective
pup
Fig.
23
Fig.
24
Я
certering
ne
Fiea
ris
Diaphragm
(CH40
only)
(Figs.
21,22)
1.
With
the 10X
objective
engaged
and
the
specimen
brought
into
focus,
turn the
field
iris
diaphragm
dial
@
counterclockwise
to
stop
down
the
diaphragm
somewhat.
(Fig.
21)
2.
Tum
the
condenser
height
adjustment
knob
@
to
bring the
field
iris
diaphragm
image
into
focus.
(Fig.
21)
3.
While
gradually
opening
the
field
iris
daphragm
image,
rotate
the
two
centering
knobs
@
of
the
compensating
lens
to
adjust
so
that
the
field
iris
diaphragm
image
is
centered
in
the
eyepiece
field
of
view.
(Figs.
21,
22)
4.
To
check
centration,
open
the
field
iris
diaphragm
until
its
image
touches
the
perimeter
of
the
field
of view.
If
the
image
is
not
precisely
inscribed
in
the
field
of
view,
center
again.
Fig.
22)
5.
When
used
for
actual
observation,
open
the
field
iris
diaphragm
until
its
image
just
circumscribes
the
field
of
view.
©
When
the
compensating
lens
and
field
iris
diaphragm
attachment
are
mounted
on the
CH30,
center
the
diaphragm
in
the
same
manner
as
described
above.
Я
Aventure
iris
Diaphragm
(Figs.
23,24)
©
The
aperture
iris
diaphragm
determines
the
numerical
aperture
of
the
illumination
system.
Matching
the
numerical
aperture
©
of
the
illumination
system
with
that
of
the
objective
provides
better
image
resolution
and
contrast,
and
also
increases
the
depth
of
focus.
©
Since
the
contrast
of
microscope
specimens
is
ordinary
low,
setting
the
condenser
aperture
iris
diaphragm
to
70-80%
of
the
NA.
of
the
Objective
in
use
is
usually
recommended.
When
necessary,
adjust
the
ratio
by
removing
the
eyepiece
and
looking
into
the
eyepiece
sleeve
while
adiusting
the
aperture
iris
diaphragm
lever
()
until
the
image
shown
in
Fig.
23
is
seen.
Fig.
25
CH30/CH40
©
sin
te
Coarse
Adjustment
Knob
Tension
(ig.25)
1.
Tho
coarse
adjustment
knob
tension
is
preadjusted
for
easy
use.
However,
if
desired,
one
can
change
the
tension
using
the
tension
adjustment
ring
O.
Applying
a
fla-bladed
screwdriver
to
any of
the
‘grooves
@
on the
circumference
of
the
ring
and
turning
the
ring
in
the
direction
of
the
arrow
increases
tension,
and
ice
versa
2.
Tho
tension
is
too
low
the
stage
drops
by
seli
or
focus
is
quickly
lost
after
adjustment
with
the
fine
adjustment
knob
G.
n
this
case,
tum
the
ring
D
in
the
direction
of
the
arrow
to
increase
tension
(EB
Pre-focusing
Lever
(0.29)
The
pre-focusing
lever
ensures
that
the
objective
does
not
come
in
contact
with
the
specimen
and
simplifies
focusing,
After
focusing
on
the
specimen
with
the
coarse
adjustment
knob,
turn
this
lever
©
clockwise
in
the
direction
of
the
arrow
to set
a
lower
limit
on
coarse
adjustment
movement
©
Focusing
with
the
fine
adjustment
knob
is
not
affected
by
this
pro-
focusing
lever.
Accordingly,
after
using
the
coarse
adjustment
knob
to
lower
the
stage
for
changing
specimens
or
applying
immersion
oil
{see
Section
5-8),
refocusing
is
easily
accomplished
by
rotating
the
coarse
adjustment
knob
to
reach
the
pre-focusing
position,
then
making
fine
adjustments
with
the
fine
adjustment
knob.
#
When
not
required,
leave
the
pre-focusing
lever
unlocked.
Use
of
Immersion
Objectives
(Fig.
27)
1.
Focus
on the
specimen
with
a
low
power
objective.
2.
Place
a
drop
of
immersion
oil
provided)
onto
the
specimen
at
the
area
to
bo
observed.
3.
Turn
the
revolving
nosepiece
to
engage
the
immersion
objective,
then
focus
using
the
fine
adjustment
knob.
+
Since any
bubbles
in
the
oil
wll
impair
the
tho
oil
is
free
of
bubbles.
a.
To
check
for
bubbles,
remove
the
eyepiece
and
fully
open
the
field
and
aperture
iris
diaphragms,
then
look
at the
exit
pupil
of
the
objective
inside
the
observation
tube.
(The
pupil
should
appear
round
and
bright.)
b.
To
remove
bubbles,
rock the
nosepiece
slightly
to
move
the
oil
immersion
objective back
and
forth
a
few
times.
Q
if
the
condenser
marking
shows
a
numerical
aperture
(NA)
of
1.0
or
more,
the
number
applies
only
when
oil
is
present
between
the
slide
glass
and
the
top
surface
of
the
condenser.
When
oil
is
not
present,
the
NA
is
about
0.9.
4.
After
use,
remove
oil
from
the
objective
front
lens
by
wiping
with
‘gauze
slightly
moistened
with
an
ether
(7
parts)
/
alcohol
(3
parts)
mixture
or
with
EE-6310,
+
Using
too
much
alcohol
can
dissolve
the
lens
adhesive.
A
Caution
on
Using
the
Immersion
Oil:
if
the
immersion
oil
comes
into
contact
with
your
eye
or
skin,
tely
tako
the
following
action.
For
aye:
Rinse
with
clean
water
(for
more
than
15
min).
For skin:
Wash
with
soap
and
water.
ho
appearance
of
the
eye
or
skin
changes
or
pain
continues,
please
consult
your
doctor.
rage,
make
sure
that
SnOdINOO
3HL
9NISn
A
>
OM
camera
back
1
{
Automatic
pRotomicrographie
system
|
PMO.
Photomicro
adapter
|
DDD"
MOML
“Rutomaile
Photomicrographic
system
PM20
===
トー
ーー
ト
lg
ane
>)
Trinocular
observation
_
tube
[A]
mas.
©
Use
the
trinocular
observation
tube
CHI-TRAS
for
photomicrography
#
Use
a
heat
absorbing
filter
(45HA)
when
using
the
PM-10, PM-20,
and
PM-30
photomicrographic
systems.
©
Selecting
the
Light
Path
(CH3-TR45)
cos
©
The
interpupilary
distance
adjustment
procedure
of
the
trinocular
‘observation
tube
(CH3-TR45)
is
different
from
that
of
the
binocular
observation
tube
(CH3-BI45).
The
interpupillary
distance
dovetai
slides
(Don
the
CH3-TRAS
should
be
moved
to
make
the
adjustment
Move
the
light
path
selector
knob
@
to
select
the
required
light path,
©
For
normal
observation,
the
light
path
selector
knob
should
be
pushed
in
For
TV
and
stil
photomicrography,
pull
out
the knob.
^
Light
path
selector
knob_]
Intensity
ratio
Application
ση
100%
for
binocular
|
Observation
of
dark
epica
ae
a
Np
#
In
case
of
long
time
exposure,
ambient
light
in
the
room
will
‘enter
through
the
observation
tube
and
the
eyepieces
and
may
create
ghost
images
or
flare.
To
block
this
extranoous
light,
dim
the
room
or
cap
the
oyopioces
or
the
focusing
telescopi
14
°
CH30/CH40
E
vounting
the
Camera
Unit
(Fig,
29)
1.
Loosen
the
clamping
screw
@
of
the
NFK
photo
eyepiece
adapter
{PMAADF)
©.
2,
Attach
the
NFK
photo
eyepiece
adapter
(PM-ADF)
©
to
the
trinocular
observation
tube
(CH3-TR45)
@
and
tighten
the
clamping
serew
©.
3.
Insert the
NFK
photo
eyepiece
(3.3X
or
5X)
@
into
the
NFK
photo
‘eyepiece
adapter
(PM-ADFI
©.
4,
Mount
the
camera
unit
directly
on
the
NFK
photo
eyepiece
adapter
(PMADF
O.
To
use
an
OM
camera
back,
mount
the
photo
eyeplece
adapter
PM-
ADF
©
{into
which
a
choice
of
NFK
photo
eyepiece
is
inserted),
the
photomicro
adapter
MOM-L
@
and
the
OM
camera
back
on
the
photo
tube
in
the
described
order.
+
Use
the
3.3X
or
BX
NFK
photo
eyepiece.
+
Focus
using
the finder
of
the
camera
back.
+
Photomicrographic
magnification
bjective
magnification
X
Photo
eyepiece
magnification,
#
To
take
a
good
photomicrography
with
a
single
lens
reflex
‘camera
back,
a
shutter
speed
at
1/2
second
or
slower
is
rec-
‘ommended
so
as
to
reduce
the
mirror-lockup
shock.
€
Focus
Adjustment
The focus
of
the
eyepieces
and
the
camera
film
plane
are
not the
‘same.
Consequently,
always
perform
camera
focusing
by
using
the
focusing
telescope
of
the
camera
unit. For
details,
referto
the
instruc-
tion
manual
of
the
photomicrographic
system.
(A
Color
Temperature
Compensation
(Fig.
30)
©
When
using
daylight
type
film
in
the
camera,
+
The
blue
filter
is
solely
designed
for
visual
observation
(day-
light)
If
this
filter
is
engaged
while
taking
photographs
with
daylight
film,
the
color
reproduction
will
be
poor.
1.
Mount
the
light
balancing
fiter
(45LBD-2N)
@
in
the
filter
holder
@
of
the
microscope
frame,
2.
Turn
the
light
intensity
knob
G).
To
obtain
illumination
close
to
day-
light,
set
the
index
of
the
knob
at
5
in
case
of
the
CH30,
and
4
in
the
case
of
CHAO,
©
Nomenciature
Light
Annuli
chansio
Cha.RS40
Faso
Contrast
Objectives
PCDATOX
PLPCOAGOXPL
Geniaring
Telescope
OTS]
<
@
Vounting
the
Phase
Contrast
Attachment
Mount
the
phase
annulus
(CH2-RS10/CH2-RS40)
in
the
same
way
as
the
filter
holder
+
Replace
previously
mounted
objectives
with
the
phase
contrast
objectives.
©
before
centration
Ator
centration
の
di
iii
Tum
the
revolving
nosepiece
to
move
the
objective
with
the
same
number
es
the
mounted
light
annulus.
Set
the
specimen
on
the
stage
and
ring
it
into
focus.
Remove
either
one
of
the
ογορίοσος
and
insert
the
centering
telescope
(CTI
into
the
eyepiece
tube
Rotate
the
CT's
upper
lens
assombly
so
that
the
bright
ing
©
light
annulus
inthe
condenser
and
the
dark
ring
(
(phase
annulus
in
the
objective)
come
into
focus.
(Fig.
31)
Rotate
the
two
ight
annulus
centering
knobs
@
unti
th
ght
annulus
and
phase
annulus
ae
concentric
and
superimposed.
(Figs.31
&
32)
Remove
the
CFS
and
insert
the
eyepiece
back
into
the
eyepiece
tube
to
perform
phase
contrast
observation.
To
increase
image
contrast,
mount
the
filter
on
the
filer
holder.
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