Olympus Ch30 User manual

OLYMPUS

INSTRUCTIONS

CH30/CH40

BIOLOGICAL

MICROSCOPE

IMPORTANT

Safety

Symbols

The following

symbols

are

found

on the

microscope.

Study

the

meaning

of

the

symbols,

and

always

use

the

microscope

in

the

safest

possible

manner.

1.

Make

sur

that

the

voltage

selector

switch

@

located

on

the

underside

of

the

base

of

the

microscope

frame

is

set

to

conform

with

the

local

‘mains

voltage.

Move

the

switch

using

an

Allen

wrench,

etc.

(Fig.

1)

©

When

shipped

from

the

factory,

the

switch

is

set

to

110-120V

and

230-240V

in

100V

and

2009

areas,

respectively.

2.

To

avoid

potential

shock

hazard

and

fire,

always

switch

the

main

switch

©

to

"O"

(OFF) and

disconnect

the

power

cord

from

the

wall

outlet

before

replacing

the

bulb.

Allow

the

lamp

housing

cover

@and

the

bull

to

cool

before

touching,

3.

Install

the

microscope

on

a

level

table.

The

air

vents

on

the

underside

of

the

base

should

never

be

blocked

such

as

by

placing

the

microscope

on

a

flexible

surface,

e.g.,

a

carpet,

etc,

as

this

could

result

in

overheating

and

cause

a

fire,

4.

Always

use

the

power

cord

provided

by

Olympus.

If

no

power

cord

is

provided,

please

select

the

proper

power

cord

by

referring

to

the

section

“PROPER

SELECTION

OF

THE

POWER

SUPPLY

CORD”

ar

the

end

of

this

instruction

manual.

If

the

proper

power

cord

is

not

used,

Olympus

can

no

longer

warrant

the

electrical

safety

and

performance

of

the

equipment

5.

Connect

the

power

cord

correctly

and

ensure

that

the

ground

terminal

of

the

power

supply

and

that

of

the

wall

outlet

are

properly

connected

H

the

equipment

is

not

grounded,

Olympus

can

no

longer

warrant

the

electrical

safety

and

performance

of

the

equipment.

6.

Never

insert

metal

objects,

etc.

into

the

air

vents

of

the

microscope

frame

as

this

will

result

in

electrical

shock

and/or

personal

injury.

Symbol

Explanation

Indicates

that

the

surface

becomes

hot,

and

should

not

be

touched

with bare

hands,

Before

use,

carefully

read

the

instruction

manual.

Improper

handling

could

result

in

injury

to

the

user

and/or

damage

to

the

equipment.

Indicates

that

the

main

switch

is

ON,

A

A

|

o

Indicates

that

the

main

switch

is

OFF.

€

Getting

Ready

1.

A

microscope

is

a

precision

instrument.

Handle

it

with care

‘and

avoid

subjecting

it

to

sudden

or

severe

impact.

2.

Do

not

use

the

microscope

where

it

is

subjected

to

direct

sunlight, high

temperature

and

humidity,

dust

or

vibrations.

(For

operating

environment

conditions,

refer

to

and

adhere

to

the

conditions

specified

in

Section

6,

SPECIFICATIONS

on

page

16)

3.

The

tension

ofthe

coarse

focus

adjustment

knob

should

only

be

adjusted

by

means

of

the

tension

adjustment

ring,

4.

Heat

from

this

microscope

is

lod

away

by

natural

convection,

Consequenti,

do

not

use

itina

constricted

space

and

ensure

that

the

room

is

well

ventilated,

5.

When

moving

the

microscope,

carry

it

with

one

hand

under

the

base

D

and

the

other

hand

holding

at

the

recessed

handle

‘on

the

rear

of

the

arm

@

as

shown

in

the

illustration

on

the

left.

Handle

the

microscope

with care

when

moving

it

*

When

moving

the

microscope,

never

hold

the

stage

or

binocular

section

of

the

observation

tube,

etc.

Also

make

sure

that

eyepieces,

specimen,

etc.

do

not

falloff.

*

Sliding

the

microscope

on the

surface

of

the

table

may

Faz

damage

or

tear

off

the

rubber

feet

and/or

scratch

the

table

top

surface.

Maintenance

and

Storage

1.

Clean

all

glass

components

by

wiping

gently

with

gauze.

To

remove

fingerprints

or

oil

smudges,

wipe

with

gauze

slightly

moistened

with

a

mixture

of

ether

(70%)

and alcohol

(80%)

*

Do

not

use

a

mixture

of

ether

(70%)

and

alcohol

(30%)

to

clean

the

lower

lens

(made

of

optical

plastic)

of

the

eyepiece

(NCWHK10X)

because

such

solvents

cloud

the

lens.

If

dust

adheres

to

the lens,

blow

it

off

or

wipe

it

away

gently

with

a

dry

cloth.

‘Since

solvents

such

as

ether and

alcohol

are

highly

flammable,

they

must

be

handled

carefully.

Be

sure

to

keep

these

chemicals

away

from open flames

or

potential

sources

of

electrical

sparks

—

for

example,

electrical

equipment

that

is

being

switched

on

or

off,

Also

remember

to

always

use

these

chemicals

only

in

@

well-ventilated

room.

2.

Do

not

attempt

to

use

organic

solvents

to

clean

the

non-optical

components

of

the

microscope.

To

clean

these,

use

a

lint-free,

soft

cloth

lightly

moistened

with

a

diluted neutral

detergent.

2.

Do

not

disassemble

any

part

of

the

microscope

as

malfunction

or

damage

may

occur

4,

When

not

using

the

microscope,

keep

it

covered

with

the

provided

dust

cover.

Warning

Labels

Warning

labels

are

affixed

at

parts

where

special

precaution

is

required

when

handling

and

using

the

microscope,

Always

heed

the

warnings,

[Caution

for

Warning

Label

Position

|

Base

Underside

"gay

po

car

If

warning

labels

become

soiled,

peel

off,

etc.

contact

your

local

Olympus

representative

to

have

them

replaced.

€

cu

I

the

microscope

is

operated

in

a

manner

not

specified

by

this

manual,

the

safety

of

the

user

may

be

imperiled.

In

‘addition,

the

equipment

may

also

be

damaged.

Always

operate

the

equipment

as

outlined

in

this

instruction

manual

CONTENTS

21

Assembly

Diagram

22

Detailed

Assembly

Procedure.

5-1

Base..

52 Stage

53

Observation

Tube

5-4

Condenser

55

Focusing

Adjustment

Knobs

5-6

Immersion

Objectives

.

57

Photomicrography

5-8

Phase

Contrast

Attachments

13

13

16

E

PROPER

SELECTION

OF

THE

POWER

SUPPLY

CORD...

2

CH30/CH40

The

diagram

below

shows

how

to

assemble

the

various

components,

The

numbers

indicate

the

order

of

assembly.

‘#

When

assembling

the

microscope,

make

sure

that

all

parts

are free

of

dust

and

dirt,

and

avoid

scratching

any

parts

or

touching

glass

surfaces.

+

Retain

the

provided

Allen

wrench.

It

should

be

used

when

units

are

replaced

or

added.

2,

Cord

rest

[optional

for

CH30/CH40)

Fiold irs

digphvagm

attachment

(optional

for

CH30)

{optional

for

CH30)

Filter

holder

(for

CH30)

1

6V,

20W

halogen

bulb

Kor

CH30)

6V,

30W

halogen

bull

tor

chao)

CH30/CH40

Я

Mounting

the

Bulb

(Replacement)

Fo.)

1.

Tum

the

microscope

frame

on

its

side

and

pull

the

lamp

housing

knob

on

the

underside

of

the

base

to

open

the

lamp

housing

cover.

2,

Holding

the

halogen

bulb

@

contained

in

the

polyethylene

bag

to

avoid

leaving

fingerprints

on

the

bulb, fully

insert

the

contact

pins

into

the

bulb

socket

@.

When

properly

seated,

pul

off

the

polyethylene

bag,

Applicable

Bulb

))

(CH30:

6V,

20W

Halogen

bulb:

GVZOW

HAL

(Philips

7388)

040:

ϐν,

30W

Halogen

bulb:

6V30W

HAL

(Philips

5761)

À

Always

use

the

designated

bulb.

Use

of

improper

bulb

may

result

in

fire,

À

Do

not

touch

the

bulb

with

bare

hands.

If

fingerprints

are

‘accidentally

left

on the

bulb,

wipe

the

bulb with

a

sof,

lint-free

cloth

moistened

with

alcohol.

Using

a

contaminated

bulb

will

shorten

the

service

life

of

the

bulb.

3.

With

the

lamp

housing

knob

stil

pulled

out,

close

the

lamp

housing

cover.

Then

push

in

the

lamp

housing

knob

to

lock

the

cover

+

The

lamp

housing

cover

cannot

be

closed

if

the

knob

is

pushed

in

before

you

attempt

to

close

cover.

<

Proc

ns

for

Bulb

Replacement

‘A

Whenever

you

replace

the

bulb

during

use

or

right

after

use,

first

move

the

main

switch

to

“O"

(OFF),

disconnect

the

power

cord

from

the

wall

outlet,

and

allow

the

bulb

and

parts

around

the

bulb

to

cool

before

touching.

#

If

the

bulb

burns

out

during

an

observation

and

needs

to

be

replaced,

remove

eyapieces,

specimen,

and

other

objects

likely

to

fall

off,

before

tilting

the

microscope

framo

to

replace

the

bulb.

Mounting

the

Observation

Tube

(Fig.

2)

1.

Using

the

provided

Allen

wrench,

loosen

the

observation

tube

clamping

screw

©,

2.

Insert

the

circular

dovetail

mount

at

the

bottom

of

the

observation

|

tube

into

the

opening

on

the

microscope

frame,

positioning

the

‘observation

tube

to

point

the

binocular

eyopieces towards

the

front

‘Clamp

the

observation

tube

by

tightening

the

clamping

screw

©.

Fig.2

№

Fig.

3

Mounting

the

Eyepieces

(Fig.

3,4)

‘Binocular

Tube

(CH3-B145),

Monocular

Tube

(CH3-MO45)

1,

Remove

the

eyepiece

dust

cap

©

(Fig.

3)

2.

Insert

the

eyepiece

@

into

the

eyepiece

sleeve

as

far

as

it

wil

go.

ig.

3)

'3,

Mount

the

other

eyepiece

in

the

same

manner.

Eyepiece

micrometer

discs

can

be

inserted

into

the

NCWHK10X

eyepieces.

Use

10

mm/100

micrometer

discs

1,

Unscrew

the

micrometer

mounting

frame

@

from

the

eyepiece

by

turning

counterclockwise.

2,

Place

a

micrometer

disc into

the

frame,

The

engraving

on

the

micrometer

disc

should

face

downward

into

the

micrometer

‘mounting

frame

@.

(Fig.

4)

3.

Screw

the

micrometer

mounting

frame

back

into

the

eyepiece

tube.

(Fig.

4)

+

Use

19

mm

diameter

micrometer

discs.

r -

[

Using

Eyepiece

Mierometer

Dises

(optional)

|-

Я

Mounting

the

Objectives

(Fig.

8)

Mount

the

objectives

on

the

revalving

nosepiece

in

such

a

manner

that

the

magnification

increases

from

low

to

higher

powers

in

a

counterclockwise

direction.

Oi

there

are

empty

sockets

with

no

objective

mounted,

make

sure

to

attach

the

provided

dust

plugs.

I

Mounting

the

Condenser

(Fig.

6)

1.

Tur

the

coarse

acustment

knob

D

to

ris

the

stage

tots

highest

postion

2.

Tum

the

condenser

height

adjustment

knob

@

to

lower

the

condenser

holder

to

the

position

where

the

clamping

screw

@

can

be

turned

3.

Insert

the

condenser

@

into

the

mounting

hole

©

and

clamp

by

tightening

the

clamping

screw

@.

Positioning

the

condenser

with

the

diaphragm

lever

@

at

the

front

facilitates

operation

4,

Turn

the

condenser

height

adjustment

knob

@

to

raise

the

condenser

holder

to

its

highest

position.

cae

CH30/CH40

Я

Mounting

the

Biue

Fitter

(ion

©

The

blue

filter

converts

the

color

temperature

of

tungsten

illumination

into

the

color

temperature of

daylight

to

show

the

specimen

in

natural

colors,

Place

the

32.5

mm

diameter

blue

filter

@

in

the

fier

holder

@ or

the

compensating

lens,

ig

ier

Hr

e

Compri

Lan

ta)

1,

Insert

the

filter holder

or

the

compensating

lens

@ into

the

condenser

from

below

until

it

seats

with

cick.

2,

When

mounting

the

compensating

lens,

place

the

lens

so

that

the

‘centering

screws © are

at

the

front,

Mounting

the

Field

Iris

Diaphragm

Attachment

(CH30

only)

(Fig.

9)

Holding

the

field

iris

diaphragm

attachment @

over

the

light

glass

exit

(O

with

the

filter

holder

notches

@

positioned

as

shown

in

Fig.

9,

insert

the

attachment

into

the

light

glass

exit

opening

untilit

seats

wath

a

click

+

Attempting

to

insert

the

attachment

with

the

notches

@

facing

in

other

directions

than

shown

in

Fig.

9

may

break

the

mount-

ing

clip.

©

Connecting

the

Power

Cord

(Figs.

101,12)

À

Cables

and

cords

are

vulnerable

when

bent

or

twisted.

Never

‘subject

them

to

excessive

force.

A

Make

sure

that the

main

switch

©

is

on

"O"

(OFF)

before

‘connecting

the

power

cord.

(Fig.

10)

A

Always

use

the

power

cord

provided

by

Olympus.

If

no

power

cord

is

provided,

please

select

the

proper

power

‘cord

by

referring

to the

section

“PROPER SELECTION

OF

THE

POWER

SUPPLY

CORD”

at

the

end

of

this

instruction

manual.

es

B

1.

Connect

the

power

cord

plug

@

to

the

AC

receptacle

®.

(Fig.

11)

¿

é

2.

Plug

the

power

cord

plug

@

into

a

wall

outlet.

(Fig.

12)

‘A

Connect

the

power

cord

correctly

and

ensure

that

the

ground

terminal

of

the

power

supply

and

that

of

the

wall

outlet

are

properly

connected.

the

equipment

is

net

grounded,

Olympus

can

no

longer

war-

rant

the

electrical

safety

and

performance

of

the

equipment.

€

Cord

Rest

(CH3-cH)

(optional)

(Figs.

13,14)

(Q

When

the

cord

røst

(CH3-CH)

is

attached

on the

rear

panel

of

the

microscope

frame,

the

power

cord

can

be

wound

around

it

for

storage.

Align

the

hooks

@

on

the

cord

rest

O

with the

air

vent

sits

at

©,

‘and

insert

the

hooks

into

the

sits

until

the

cord

rest

is

pressed

against

the

rear

panel

ofthe

microscope

frame.

Then

pull

the

cord

rest

down

to

secure

it.

Note:

The

illustration

of

the

cord

rest

only

shows

the

sections

relevant

for

the

explanation

here.

The

actual

cord

rest

looks

somewhat

different.

+

Do

not

grasp

the

cord

rest

when

moving

the

microscope.

The

cord

rest

may

suddenly

come

loose

and

you

may

drop

the

microscope

causing

material

damage

or

personal

injury.

Detaching—Move

the

microscope

frame

to

the

edge

of

the

table

©.

Then

apply

@

screwdriver

@

or

Allan

wrench

to

the

lower

portion

of

the

cord

rest

©)

and

push

in

the [1]

directions

to

move

the

entire

cord

rest

upward

to

allow

it

to

be

detached.

Fig.

14

이

commas

CH30/CH40

“Field

iris

diaphragm

dial

RVATION

PROCEDUR

Switch

the

main

switch

to

"I"

(ON)

and

adjust

the

brightness

with

the

light

intensity

knob.

(Page

10)

“Tum

the

revolving

nosepiece

to

engage

the

10X

objective.

Make

sure

that

the

revolving

nosepiece

stops

with

an

audible

click

Place

a

specimen

on the

stage.

(Page

10)

Turn

the

X-axis

knob

© and

Y-axis

knab

@

to

move

the

specimen

into

the

light

path.

(Page

11)

Looking

through

the

right

eyepiece

with

your

right

eye,

turn

the

coarse

adjustment

knob to

bring

the

specimen

into

focus.

After

obtaining

approximate

focus,

use

the

fine

adjustment

knob

to

make

fine

adjustments.

Looking

through

the

left

eyepiece

with

your

left

eye,

turn

the

diopter

adjustment

ring

to

focus

the

specimen.

(Page

11)

CH30/CH40

7.

Adjust

the

interpupillay

distance

of

the

eyepleces.

(Page

11)

8.

Center

the

field

iris

diaphragm

by

manipulating

the

two

centering

screws,

on the

compensating

lens.

(CH40

only)

(Page

12)

In

case

of

CH30,

centration

is

not

necessary.

If

the

optional

field

i

diaphragm

attachment

is

mounted,

center

the

attachment

in

the

same

manner

as

described

above.

9.

Engage

the

objective

to

be

used

for

observation

and

adjust

the

light

n-

tensity

to

the

desired

level,

then

readjust

the

focus.

10.

Adjust

the

field

iris

diaphragm.

This

only

applies

to

the

CH30

if

the

optional

field

iris

diaphragm

attach-

ment

is

mounted.

S3HNG300H4

NOLLVAHASEO

40

AHVAINNS

11.

Adjust

the

aperture

iris

diaphragm.

(Page

12)

rr

5

10

A

turning

on

the

Bulb

(Fig, 15)

1.

Switch

the

main

switch

@

to

“1”

(ON)

2.

Turning

the

light

intensity

knob

@

clockwise

in

the

direction

of

higher

numbers

makes

the

illumination

brighter,

and

vice

versa,

Field

Iris

Diaphragm

(CH40

only)

(Fig.

18)

Using

the

field

iris

diaphragm

dial

©,

adjust

the

diameter

of

the

field

its for

objective

power

to

the

extent

that

it

just

cireumscribes

the

field

of

view.

When

the

field

iris

diaphragm

is

stopped

down

to

circum-

scribe

the

field

of

view,

it

will

exclude

extraneous

light

and

improve

image

contrast

within

the

field

of

view.

+

When

using

100X

objective,

the

field

iris

diaphragm

image

will

not

be

visible

within

the

field

of

view.

Accordingly,

stop

down

the

diaphragm

to

its

smallest

diameter.

On

case

of

CH30,

the

field ins

diaphragm

attachment

is

mounted

on

the

light

exit

glass

of

the

microscope

base.

©

Specimen

Placement

(Fiom

+

Releasing

the

curved

finger

with

great

force,

or

suddenly

re-

leasing

your

grip

on

the

curved

finger

knob

while

releasing

the

curved

finger,

will

crack

or

damage

the

slide

glass.

Always

release

the

curved

finger

with

great

car

‘Specimen

Holder

for

Single

Slides

1.

Tüm

the

coarse

adjustment

knob

@

counterclockwise

in

the

direc-

tion

of

the

arrow

to

lower

the

stage.

2.

Open

the

springoaded

curved

finger

@

on

the

specimen

holder

and

slide

the

specimen

slide

into

the

specimen

holder

from

the

front.

3.

After

placing

the

slide

as

far

as

it

will

go,

gently

release

the

curved

finger

O.

‘Specimen

Holder

for

2

Specimen

Slides

1.

Place

the

first

specimen

slide

as

described

in

steps

1

and

2

above.

Then

place

the

second

specimen

slide so

that

it

contacts

the

first

specimen

slide,

2.

Gently

release

the

curved

finger

©.

CH30/CH40

Placing

the

Specimen

Slide

with

One

Hand

Place

the

specimen

slide

at

the

front

of

the

stage,

then

slide the

specimen

slide

on

the

stage

surface

to

slowly

and

gradually

open

the

curved

finger

in

the

direction

of

the

arrow.

Insert the

specimen

slide

into

the

specimen

holder

Until

itis

fully

and

properly

seated

in

the

specimen

holder.

Use

cover

glasses

of

0.17

mm

thickness

in

combination

with

objectives

marked

with

the

“160/0.17" engraving

for

optimum

performance

of

these

objectives.

‘Specimen

slides

with

a

thickness

between

0.9

mm

and

1.2

mm

are

recommended

for

CH30/CH40

microscopes

Using

thicker

specimen

sides

may prevent

correct

formation

of

the

field

iris

diaphragm

image

on the

specimen

surface

and

illumination

will

not

be

correct

in

case

of

darkfield

observation

Fig.

20

Moving

the

Specimen

(Fig.

18)

Turning

the

Y-axis

knob

@

moves

the

specimen

in

the

Y-axis

direc-

tion.

Turing

the

X-axis

knob

@

moves

the

specimen

in

the

X-axis

direction.

*

Never

move

the

specimen

by

holding

the

specimen holder

or

‘stage

directly

as

this

will

damage

the

knob

rotation

mechanism.

k

When

further

movement

of

the

stage

or

specimen

holder

is

prevented

by

the

provided

limiting

mechanism,

the

tension

of

the

Y-axis

and

X-axis

knob

rotation

will

increaso.

At

this

point,

iting

mechanism.

©

Adiusting

the

interpupitiary

Distance

(Fig.

19)

While

looking

through

the

eyepieces,

adjust

for

binocular

vision

until

the

left

and

right

fields

of

view

coincide

completely.

Adjust

so

that

the

two

index

dots

+

are

horizontal.

‘©

Placing

the

interpupilary

distance

indexes

so

that

they

are

parallel

with

the

horizontal

lines

faciitates

the

adjustment

Also

keep

the

dots

parallel

with

the

horizontal

lines

in

case

of

interpupilary

distances

other

than

60, 60, 70,

and

76.

©

Nate

your

interpupilary

distance

so

that

it

can

be

quickly

duplicated

Diopter

Adjustment

(Fig.

20)

1.

Looking

through

the

right

eyepiece

with

your

right

eye,

rotate

the

coarse

and

fine

adjustment

knobs

to

bring

the

specimen

into

focus.

2.

Looking

through

the

left

eyepiece

with your

left

eye,

turn

the

diopter

adjustment

ring

©

to

focus

the

specimen.

11

12

Fig.

21

ση

apn

image

Eyepiece

FE

Se

Objective

pup

Fig.

23

Fig.

24

Я

certering

ne

Fiea

ris

Diaphragm

(CH40

only)

(Figs.

21,22)

1.

With

the 10X

objective

engaged

and

the

specimen

brought

into

focus,

turn the

field

iris

diaphragm

dial

@

counterclockwise

to

stop

down

the

diaphragm

somewhat.

(Fig.

21)

2.

Tum

the

condenser

height

adjustment

knob

@

to

bring the

field

iris

diaphragm

image

into

focus.

(Fig.

21)

3.

While

gradually

opening

the

field

iris

daphragm

image,

rotate

the

two

centering

knobs

@

of

the

compensating

lens

to

adjust

so

that

the

field

iris

diaphragm

image

is

centered

in

the

eyepiece

field

of

view.

(Figs.

21,

22)

4.

To

check

centration,

open

the

field

iris

diaphragm

until

its

image

touches

the

perimeter

of

the

field

of view.

If

the

image

is

not

precisely

inscribed

in

the

field

of

view,

center

again.

Fig.

22)

5.

When

used

for

actual

observation,

open

the

field

iris

diaphragm

until

its

image

just

circumscribes

the

field

of

view.

©

When

the

compensating

lens

and

field

iris

diaphragm

attachment

are

mounted

on the

CH30,

center

the

diaphragm

in

the

same

manner

as

described

above.

Я

Aventure

iris

Diaphragm

(Figs.

23,24)

©

The

aperture

iris

diaphragm

determines

the

numerical

aperture

of

the

illumination

system.

Matching

the

numerical

aperture

©

of

the

illumination

system

with

that

of

the

objective

provides

better

image

resolution

and

contrast,

and

also

increases

the

depth

of

focus.

©

Since

the

contrast

of

microscope

specimens

is

ordinary

low,

setting

the

condenser

aperture

iris

diaphragm

to

70-80%

of

the

NA.

of

the

Objective

in

use

is

usually

recommended.

When

necessary,

adjust

the

ratio

by

removing

the

eyepiece

and

looking

into

the

eyepiece

sleeve

while

adiusting

the

aperture

iris

diaphragm

lever

()

until

the

image

shown

in

Fig.

23

is

seen.

Fig.

25

CH30/CH40

©

sin

te

Coarse

Adjustment

Knob

Tension

(ig.25)

1.

Tho

coarse

adjustment

knob

tension

is

preadjusted

for

easy

use.

However,

if

desired,

one

can

change

the

tension

using

the

tension

adjustment

ring

O.

Applying

a

fla-bladed

screwdriver

to

any of

the

‘grooves

@

on the

circumference

of

the

ring

and

turning

the

ring

in

the

direction

of

the

arrow

increases

tension,

and

ice

versa

2.

Tho

tension

is

too

low

the

stage

drops

by

seli

or

focus

is

quickly

lost

after

adjustment

with

the

fine

adjustment

knob

G.

n

this

case,

tum

the

ring

D

in

the

direction

of

the

arrow

to

increase

tension

(EB

Pre-focusing

Lever

(0.29)

The

pre-focusing

lever

ensures

that

the

objective

does

not

come

in

contact

with

the

specimen

and

simplifies

focusing,

After

focusing

on

the

specimen

with

the

coarse

adjustment

knob,

turn

this

lever

©

clockwise

in

the

direction

of

the

arrow

to set

a

lower

limit

on

coarse

adjustment

movement

©

Focusing

with

the

fine

adjustment

knob

is

not

affected

by

this

pro-

focusing

lever.

Accordingly,

after

using

the

coarse

adjustment

knob

to

lower

the

stage

for

changing

specimens

or

applying

immersion

oil

{see

Section

5-8),

refocusing

is

easily

accomplished

by

rotating

the

coarse

adjustment

knob

to

reach

the

pre-focusing

position,

then

making

fine

adjustments

with

the

fine

adjustment

knob.

#

When

not

required,

leave

the

pre-focusing

lever

unlocked.

Use

of

Immersion

Objectives

(Fig.

27)

1.

Focus

on the

specimen

with

a

low

power

objective.

2.

Place

a

drop

of

immersion

oil

provided)

onto

the

specimen

at

the

area

to

bo

observed.

3.

Turn

the

revolving

nosepiece

to

engage

the

immersion

objective,

then

focus

using

the

fine

adjustment

knob.

+

Since any

bubbles

in

the

oil

wll

impair

the

tho

oil

is

free

of

bubbles.

a.

To

check

for

bubbles,

remove

the

eyepiece

and

fully

open

the

field

and

aperture

iris

diaphragms,

then

look

at the

exit

pupil

of

the

objective

inside

the

observation

tube.

(The

pupil

should

appear

round

and

bright.)

b.

To

remove

bubbles,

rock the

nosepiece

slightly

to

move

the

oil

immersion

objective back

and

forth

a

few

times.

Q

if

the

condenser

marking

shows

a

numerical

aperture

(NA)

of

1.0

or

more,

the

number

applies

only

when

oil

is

present

between

the

slide

glass

and

the

top

surface

of

the

condenser.

When

oil

is

not

present,

the

NA

is

about

0.9.

4.

After

use,

remove

oil

from

the

objective

front

lens

by

wiping

with

‘gauze

slightly

moistened

with

an

ether

(7

parts)

/

alcohol

(3

parts)

mixture

or

with

EE-6310,

+

Using

too

much

alcohol

can

dissolve

the

lens

adhesive.

A

Caution

on

Using

the

Immersion

Oil:

if

the

immersion

oil

comes

into

contact

with

your

eye

or

skin,

tely

tako

the

following

action.

For

aye:

Rinse

with

clean

water

(for

more

than

15

min).

For skin:

Wash

with

soap

and

water.

ho

appearance

of

the

eye

or

skin

changes

or

pain

continues,

please

consult

your

doctor.

rage,

make

sure

that

SnOdINOO

3HL

9NISn

A

>

OM

camera

back

1

{

Automatic

pRotomicrographie

system

|

PMO.

Photomicro

adapter

|

DDD"

MOML

“Rutomaile

Photomicrographic

system

PM20

===

トー

ーー

ト

lg

ane

>)

Trinocular

observation

_

tube

[A]

mas.

©

Use

the

trinocular

observation

tube

CHI-TRAS

for

photomicrography

#

Use

a

heat

absorbing

filter

(45HA)

when

using

the

PM-10, PM-20,

and

PM-30

photomicrographic

systems.

©

Selecting

the

Light

Path

(CH3-TR45)

cos

©

The

interpupilary

distance

adjustment

procedure

of

the

trinocular

‘observation

tube

(CH3-TR45)

is

different

from

that

of

the

binocular

observation

tube

(CH3-BI45).

The

interpupillary

distance

dovetai

slides

(Don

the

CH3-TRAS

should

be

moved

to

make

the

adjustment

Move

the

light

path

selector

knob

@

to

select

the

required

light path,

©

For

normal

observation,

the

light

path

selector

knob

should

be

pushed

in

For

TV

and

stil

photomicrography,

pull

out

the knob.

^

Light

path

selector

knob_]

Intensity

ratio

Application

ση

100%

for

binocular

|

Observation

of

dark

epica

ae

a

Np

#

In

case

of

long

time

exposure,

ambient

light

in

the

room

will

‘enter

through

the

observation

tube

and

the

eyepieces

and

may

create

ghost

images

or

flare.

To

block

this

extranoous

light,

dim

the

room

or

cap

the

oyopioces

or

the

focusing

telescopi

14

°

CH30/CH40

E

vounting

the

Camera

Unit

(Fig,

29)

1.

Loosen

the

clamping

screw

@

of

the

NFK

photo

eyepiece

adapter

{PMAADF)

©.

2,

Attach

the

NFK

photo

eyepiece

adapter

(PM-ADF)

©

to

the

trinocular

observation

tube

(CH3-TR45)

@

and

tighten

the

clamping

serew

©.

3.

Insert the

NFK

photo

eyepiece

(3.3X

or

5X)

@

into

the

NFK

photo

‘eyepiece

adapter

(PM-ADFI

©.

4,

Mount

the

camera

unit

directly

on

the

NFK

photo

eyepiece

adapter

(PMADF

O.

To

use

an

OM

camera

back,

mount

the

photo

eyeplece

adapter

PM-

ADF

©

{into

which

a

choice

of

NFK

photo

eyepiece

is

inserted),

the

photomicro

adapter

MOM-L

@

and

the

OM

camera

back

on

the

photo

tube

in

the

described

order.

+

Use

the

3.3X

or

BX

NFK

photo

eyepiece.

+

Focus

using

the finder

of

the

camera

back.

+

Photomicrographic

magnification

bjective

magnification

X

Photo

eyepiece

magnification,

#

To

take

a

good

photomicrography

with

a

single

lens

reflex

‘camera

back,

a

shutter

speed

at

1/2

second

or

slower

is

rec-

‘ommended

so

as

to

reduce

the

mirror-lockup

shock.

€

Focus

Adjustment

The focus

of

the

eyepieces

and

the

camera

film

plane

are

not the

‘same.

Consequently,

always

perform

camera

focusing

by

using

the

focusing

telescope

of

the

camera

unit. For

details,

referto

the

instruc-

tion

manual

of

the

photomicrographic

system.

(A

Color

Temperature

Compensation

(Fig.

30)

©

When

using

daylight

type

film

in

the

camera,

+

The

blue

filter

is

solely

designed

for

visual

observation

(day-

light)

If

this

filter

is

engaged

while

taking

photographs

with

daylight

film,

the

color

reproduction

will

be

poor.

1.

Mount

the

light

balancing

fiter

(45LBD-2N)

@

in

the

filter

holder

@

of

the

microscope

frame,

2.

Turn

the

light

intensity

knob

G).

To

obtain

illumination

close

to

day-

light,

set

the

index

of

the

knob

at

5

in

case

of

the

CH30,

and

4

in

the

case

of

CHAO,

©

Nomenciature

Light

Annuli

chansio

Cha.RS40

Faso

Contrast

Objectives

PCDATOX

PLPCOAGOXPL

Geniaring

Telescope

OTS]

<

@

Vounting

the

Phase

Contrast

Attachment

Mount

the

phase

annulus

(CH2-RS10/CH2-RS40)

in

the

same

way

as

the

filter

holder

+

Replace

previously

mounted

objectives

with

the

phase

contrast

objectives.

©

before

centration

Ator

centration

の

di

iii

Tum

the

revolving

nosepiece

to

move

the

objective

with

the

same

number

es

the

mounted

light

annulus.

Set

the

specimen

on

the

stage

and

ring

it

into

focus.

Remove

either

one

of

the

ογορίοσος

and

insert

the

centering

telescope

(CTI

into

the

eyepiece

tube

Rotate

the

CT's

upper

lens

assombly

so

that

the

bright

ing

©

light

annulus

inthe

condenser

and

the

dark

ring

(

(phase

annulus

in

the

objective)

come

into

focus.

(Fig.

31)

Rotate

the

two

ight

annulus

centering

knobs

@

unti

th

ght

annulus

and

phase

annulus

ae

concentric

and

superimposed.

(Figs.31

&

32)

Remove

the

CFS

and

insert

the

eyepiece

back

into

the

eyepiece

tube

to

perform

phase

contrast

observation.

To

increase

image

contrast,

mount

the

filter

on

the

filer

holder.

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