Olympus Fluoview FV1000 User manual

User’s Manual
FLUOVIEW
FV1000
CONFOCAL LASER SCANNING
BIOLOGICAL MICROSCOPE
[OPERATION] FV10-SW Ver 5.0c
This user’s manual is for the software to be run on Olympus FLUOVIEW FV1000 Confocal Laser
Scanning Biological Microscope. To ensure safety, obtain optimum performance and familiarize
yourself fully with this product, we recommend that you study this manual thoroughly before operation.
This user’s manual is composed of two volumes including “OPERATION INSTRUCTIONS” and
“MAINTENANCE”. Together with this manual, please also read the “SAFETY GUIDE”, “HARDWARE
GUIDE” of User’s manual FLUOVIEW FV1000 and the instruction manual of the microscope in order to
understand overall operation methods. To ensure the safety operation of laser system, we recommend
you to study the manual of each laser and the light source equipment besides this manual.
Retain this manual in an easily accessible place near a system for future reference.
Petition
A
X7274

CAUTION
1
Page
CAUTION
1. Reproduction, copying or duplication of a part or all of this software and manual is prohibited.
2. The information described in this manual may be subject to change without notice.
Registered Trademarks
Microsoft, Microsoft Windows, Excel for Windows are registered trademarks of Microsoft Corporation.
Other brand names and product names are trademarks or registered trademarks of their respective owners.

FLUOVIEW MANUAL CONFIGURATION
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FLUOVIEW MANUAL CONFIGURATION
The FLUOVIEW system uses two manuals including this “User’s Manual” and the on-screen
manual built into the software (“Online Help”).
The User’s Manual is composed of the five following volumes and subject matter:
OPERATION INSTRUCTIONS
Describes the operation procedures of the FLUOVIEW system, for example, methods
for image acquisition and various image processing.
1 Getting Started FLUOVIEW................................................................................ 1-1
2 APPLIED OPERATIONS.................................................................................... 2-1
Appendix A List of Hot Keys.................................................................................. A-1
Appendix B Glossary ............................................................................................. B-1
Appendix C USER REGISTRATION OF FV1000 ................................................. C-1
Appendix D Change of Default Folder for [File I/O] Panel..................................... D-1
Appendix E List of Functions in the [Active Overlays] Dialog Box......................... E-1
Appendix F Hand Switch and Microscope Frame Function Allocation .................. F-1
MAINTENANCE
Describes maintenance of the FLUOVIEW system.
1 Software Setup................................................................................................... 1-1
2 Maintenance of Major System Units................................................................... 2-1
3 Setting the Confocal Aperture ............................................................................ 3-1
TROUBLESHOOTING
Describes countermeasures in case trouble occurs.
1 TROUBLESHOOTING GUIDE ........................................................................... 1-1
For Online Help, please see “1-3 Online Help” in “OPERATION INSTRUCTIONS” of this
manual.

NOTATIONS IN THIS MANUAL
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Page
NOTATIONS IN THIS MANUAL
This manual complies with the following notations.
Notation of Caution, Notes and Tips
Notation Description
Caution to prevent injuries to the user or damage to the
product (including surrounding objects).
NOTE
Note for the user.
TIP
Hint or one-point advice for user reference.
Notation of panel, Command Buttons and Dialog Boxes
Notation Description
[Acquire] panel The name of a panel, dialog box, list box or check box is
enclosed inside square brackets ([ ]).
<OK> button
<Open File> button
The name of a button in a panel or dialog box is enclosed
inside angled brackets (< >).
Notation of Mouse Operations
Notation Description
clicking Action of pressing, then immediately releasing the mouse
button.
double-clicking Action of clicking the mouse button twice in quick succession.
dragging Action of moving the mouse while holding down the mouse
button, then releasing the mouse button at the desired
destination.
(Note) In this manual, clicking, double-clicking and dragging involves pressing the left button
of the mouse, unless otherwise specified.

NOTATIONS IN THIS MANUAL
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Notation of key operations
Notation Description
Enter The name of a key is enclosed inside .
F1
+
Alt The positive sign (+) expresses the combination of more than
one key operation.
For example, F1
+
Alt refers to pressing the
F1 key while holding the Alt key down.
Direction keys Generic names given to the →, ←, ↑and
↓keys.
Notation of system-specific terms
Notation Description
XY observation
(Other observations)
Refers to observation with XY scanning.
(The same principle also applies to other observations such
as XZ, Xt, XYZ, XYt and XYZt.)
Note that some of the panels and dialog boxes shown in this manual are not the precise
reproductions of the originals. Some windows are resized to facilitate the reading and some
grayed-out characters are printed in readable characters.

Software Functional Configuration
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Page
Software Functional Configuration
This software uses panel-type windows.
Usually, it is required to “select a menu then select the command to be executed” in order to
execute a function provided by software. With the panel system, a software function can be
executed easily by “selecting the panel page tab of the function to be executed”, just like
when using a system notebook or file folder.
Function Window and Image Window
The FLUOVIEW software is organized by two kinds of windows, the function windows and
the image .window.
The function windows include the [Acquire], [File I/O], [Tile], [Process], [Analyze] and
[Visualize] panels.
The image .window shows either the [Live] panel or the panel image loaded from a file
([(filename)] window).
In this manual, the function windows are referred to simply using their
page tabs.
Namely, the [File I/O] panel of function windows is referred to simply as the
[File I/O] panel.
NOTE
The function window
The image window

Software Functional Configuration
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Panel Structure of the Software
This software cannot show the all function panels at a glance.Please use the following list of
the panels for reference in scrolling.
Acquire
Settings
Z Stage
Sets up the image acquisition and executes actual acquisition.
Sets the zooming ratio and observation mode for image acquisition.
Sets the microscope light path for image acquisition.
Sets the Z-direction scanning range for image acquisition.
Time Series Sets the interval period for image acquisition.
File I/O
Tile
Process
Math
Filters
Histogram
Experiment Editor
Analyze
Single
Saves, loads and deletes images.
Changes the image display method.
Processes the acquired images.
Performs mathematical and logical operations between images.
Filters images.
Changes the image contrast.
A
ppends two images, adds and extracts the channel.
A
nalyzes image data.
Obtains the intensity values, intensity distribution, length, area and
average intensity in images.
Orientation
Other Options
Series Obtains the change in the sum of intensity values in images.
Visualize Constructs an image from a different viewpoint or displays a 3D image.
Sets the image rotation angle, direction and number.
Sets the various 3D Rendering.
Dyes Sets the fluorescence dye method for image acquisition and reagent for
each channel.
Lasers Sets the intensity of laser and other option for image acquisition.

Software Functional Configuration
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Icons Executed by Dragging & Dropping
This software selects image files and observation methods (dye name) by means of
dragging & dropping. This allows simple selection based on an intuitive operation of
“selecting an icon (image file or observation method), dragging it to the desired position and
dropping it there”.

Software Functional Configuration
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Identification of Images Depending on the Observation Methods
On many occasions, FLUOVIEW displays image
icons to allow identification of the observation
method used when each image is acquired. (See
table on the left.)
When the [File I/O], [Tile], [Process], [Analyze] or
[Visualize] panel is selected, the icon of the image
selected in the image window is displayed in a
frame at the top of the function panel. The image
icons are also displayed in the [Icon] field in the
[Files] list box in the [File I/O] panel or during
dragging of an image file.
Use these icons to identify the observation methods
used in image acquisition.
In all observation modes, the icons for 3
or more channels are identical.
Image Icon Significance
XZ observation
XZ observation, 2-channel mode
Xt observation
Xt observation, 2-channel mode
XZT observation
XZT observation, 2-channel
mode
XY observation
XY observation, 2-channel mode
XYt observation
XYt observation, 2-channel
mode
XYZ observation
XYZ observation, 2-channel
mode
XYZt observation
XYZt observation, 2-channel
mode
Point Scan
Animation image
Stereo 3D image: Image to be
viewed with color eyeglasses.
3 or more channels
TIP