Pacific Image Electronics Glite 965BW User manual

User’s Manual
Glite 965BW Gel Doc System

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Table of Contents:
PACKAGE CONTENTS………………………………………………………………………………………………………3
SCANNER COMPONENTS………………………………………………………………………………………………..3
SETUP…………………………………………………………………………………………………………………………….4
TO SCAN A FLUORESCENCE-STAINED GEL………………………………………………………………………..5
TO SCAN WESTERN BLOT/COLORIMETRIC STAINED GEL………………………………………………….6
BROWSE SCANNED IMAGES/DELETE SCANNED IMAGES
Examine Scanned Images…………………………………………………………………………………….7
Delete Scanned Images……………………………………………………………………………………….8
INSTALLATION OF AMBER FILTER SHEET………………………………………………………………………….9
DISPLAY SCANS ON MONITOR OR PROJECTOR……………………………………………………………….10
FUNCTION MENU OPTIONS
A. Function Menu Options I………………………………………………………………………………11
B. Function Menu Options II……………………………………………………………………………..13
C. Function Menu Options I: Operation…………………………………………………………….14
D. Function Menu Options II: Operation…………………………………………………………...18
SPECIFICATIONS………………………………………………………………………………………………..…………..22

PACKAGE CONTENTS
» Scanner unit
» User’s manual
» QIG
» Gel shovel
» A/C adapter (12V, 4.16A, DC)
» Amber filter sheet (in box)
» Black plastic sheet (in box)
» Flash Drive (16G) USB. 3.0
SCANNER COMPONENTS
Front (Figure 1):
» Up/Down, Left/Right Menu Button
» USB 2.0 Port
» Scan Button
» Blue Light/White Light Selection Switch
» Power On/Off Button
» LCD Display
» Upper Lid
Back (Figure 2):
» DC power inlet
» USB 2.0 port
» VGA Out port
Up/Down, Left/Right menu button
USB 2.0 Port
Scan button
Blue Light/White Light selection switch
Power Button
LCD display
Upper lid
Figure 1
Figure 2
USB 2.0 port
DC power
Inside (Figure 3):
» Upper Lid
» Lighting Area
» Lid Sensor Figure 3
Upper lid
Lighting area
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VGA Out Port
Lid Sensor

SETUP
1. Remove all tapes that hold items in place for shipping.
2. Place the Glite 965BW unit on a flat surface.
3. Connect the included AC adapter to the back of the unit then plug into an electrical outlet.
4. Push power button.
5. Insert a USB flash drive.
6. The Menu Mode icon will be displayed on top left side of the display (Figure 4).
7. Place a gel to the center of the surface of the lighting area (Figure 5, 6).
8. Adjust image conditions then scan.
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Figure 5
Place the gel directly
Menu mode icon
Figure 4
Place gel to the center of the lighting area
Figure 6

TO SCAN A FLUORESCENCE-STAINED GEL
1. In “Scan Mode” lift the lid to open the
scanner.
2. Slide the black plastic sheet into the groove of the
white backlight to block the white light on the lid*
(Figure 7).
3. Close the scanner lid.
4. Slide the Light Selection Switch on the front side
of the scanner to choose blue light source for
scanning stained gel.
5. Set and adjust the desired image quality through
the Function Menu if necessary (Please check
“Function Menu Options” section for the details of
all functions).
6. Press SCAN button, green LED will flash and
“CAPTURING” will be displayed on the screen (If
thermal printer has been connected to the
scanner, “Printing Yes/No” option will be
displayed on the screen.). The scanned image will
be automatically saved to the USB flash drive.
7. Scanning of the gel sample is now complete.
(*It is suggested to slide the black plastic sheet to block the white backlight on
the lid for fluorescence stained gel scanning.)
Figure 7
Slide the black plastic sheet to block the background light for
fluorescence stained gel scanning.
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Black plastic sheet
White backlight

TO SCAN WESTERN BLOT/COLORIMETRIC STAINED GEL:
1. Slide the Light Selection Switch on the front side of the scanner to select the white light
source for stained gel scanning.
2. In “Scan Mode” , lift the lid to open the scanner.
3. Make sure there is no black plastic sheet blocked in front of the white back light.
4. Place the imaging subject to the center of the lighting area.
5. Close the scanner lid.
6. Set “White Epi”or “White Back Light”* item through Function Menu (Please check “LED
Lighting” in “Function Menu Options” section for turning on “White Epi” or “White Back
Light” options).
7. Adjust the desired image quality through the Functions Menu if necessary (Please check
“Function Menu Options” section for the details of all functions).
8. Press SCAN button, green LED will flash and “CAPTURING” will be displayed on the screen
(If thermal printer has been connected to the scanner, “Printing Yes/No” will be displayed
on the screen.).
9. The scanned image will automatically be saved to USB flash drive.
Scanning of the blot is now complete.
* “White Back Light” mode is to scan the gel such as SDS-PAGE stained with coomassie blue and further in a
transparent background preservation. 7

BROWSE SCANNED IMAGES/DELETE SCANNED IMAGES
Examine Scanned Images
a. When icon displays on top left corner of the screen, press “OK” to get into
functions menu.
b. When “Scan Mode” icon is highlighted , press “OK” again to enter “Play Mode”.
c. The “Play Mode” icon will display on top left corner of screen. The captured
images can then be reviewed one by one by pressing right/left arrow buttons.
d. Press up/down arrow button, the image can be zoomed in/out and be moved
upward/downward and leftward/rightward.
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BROWSE SCANNED IMAGES/DELETE SCANNED IMAGES
Delete Scanned Images
a. When viewing the image that needs to be deleted, press “OK” to get into
Function Description II.
b. Press right arrow button to choose “Edit Mode”
c. Press up/down arrow button to choose “Delete”. Press right arrow button to select (yes/no)
to delete the file.
d. Press up/down arrow button to select “Yes”. Press “OK” to save the setting. The image
is thus deleted.

INSTALLATION OF AMBER FILTER SHEET
Glite 965BW is equipped with an amber filter sheet
for blue light protection and gel cutting purposes.
Open the lid and install the filter sheet so the gel
cutting task can be conducted.
1. Open the lid, blue light will automatically turn off.
2. Lift the lid to its maximum opening angle
3. Position the hole of the amber filter sheet in front
of the hanging tab before hanging on the lid
(Figure 8).
4. Place the amber filter sheet onto the filter
hanging tab so the filter sheet hangs as shown in
Figure 9.
5. After hanging the filter on lid, make sure the two
bent areas placed against the lid.
6. Once the installation is completed, one may turn
on the blue LED by pressing “Scan button” for
further extensive experimental procedures.
Figure 8
Figure 9
Filter sheet hanging tab
Amber Filter sheet

DISPLAY SCANS ON MONITOR OR PROJECTOR
Images from the scanner can be displayed on a television rather than on the built-in scanner
preview screen using the VGA Out port.
1. Set the television to VGA mode (see monitor documentation for details).
2. Connect the VGA cable to the video port of the TV monitor.
3. Connect the other end of the VGA cable to the VGA port on the back of the scanner.
4. Video output from the scanner can now be seen on the connected monitor including the full
menu interface.
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FUNCTION MENU OPTIONS: When images display on screen in yellow border, press “OK” button
so the functional icon will show on the left upper corner.
A. Function Menu Options I:
Scan Mode: The object is able to be scanned in this mode.
LED Lighting Mode: To select the white light application for
White Epi/White Back Light image scan, respectively (Note: this mode is available
only when white light application is selected.)
Exposure Mode: To adjust the exposure time frame in this mode.
Target Type Mode: To scan/display the image in monochrome/colorimetric and
normal/invert mode. Default setting: monochrome (black & white) in normal condition.
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Set Up Mode: To set up the following parameters:
a. LCD: Dark, Normal, Bright
b. Display: LCD, VGA
c. Video Output: 800x600, 1024x768
d. Filename: Edit scanned file name (6 characters maximum)
e. Save Format: TIF, JPG, BMP (“JPG” is default setting)
f. Quick View: 1 sec, 2 sec, 3 sec, off (“2 sec” is default setting)
g. Slide Show Cycle: 2 sec, 3 sec, 5 sec, 10 sec (“3 sec” is default setting)
h. Power Save: 1 min, 3 min, 5 min, 10 min, off (“1 min” is default setting)
i. Language: English, Japanese
j. Date: setup date yyyy/mm/dd
k. Time: setup time hh/mm/ss
l. Reset: Yes, No. (If “YES”, reset all current settings back to factory default)
M. About: Unit Name, S/W version, F/W version, Update, CWD information
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Function Menu Options:
B. Function Menu Options II:
Play Mode: To play the captured images. To examine the captured image.
Move/Zoom Mode: Zoom in/out captured images by pressing
buttons. By pressing up/down arrow button and left/right arrow
button, the image can be moved upward/downward and
leftward/rightward.
Press to exit the Move/Zoom Mode.
Auto Play Mode: To automatically play the captured images one by one.
Edit Mode: To edit the captured images (such as to rotate, to flip, or to delete the
captured image, etc…)
Preview Mode: To preview the captured images in a multi image thumbnail panel.
Set Up Mode: Same as the “Set Up Mode” functions in Function Menu Options I.
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C. Function Menu Options I: Operation
“Scan Mode”
When icon displays on the top left corner of the screen, the object is ready to be scanned.
“LED Lighting Mode” *
a. When in “Scan Mode”, press “OK” to get into functional menu option list.
b. Press right arrow button to choose “LED Lighting Mode” . ”
c. Press up or down arrow button to choose light source (White Epi & White Back Light1).
d. Press “OK” to save the setting.
*: “LED Lighting Mode” Is selectable when in white light application.
1: “White Back Light” mode is to scan gels, such as SDS-PAGE gels stained with coomassie blue,
preserved in a transparent manner. 15

“Exposure Mode”
a. When icon displays on the top left corner of the screen, press “OK” to get into functional
option list.
b. Press right arrow button to choose “Exposure Mode” .
c. Press up or down arrow button to choose the desired exposure level.
d. When the desired exposure level is selected, press “OK” to get back to “Scan mode”.
“Target Type Mode”
a. When icon displays on the top left corner of the screen, press “OK” to get into functional
menu option list.
b. Press right arrow button to choose “Target Type Mode”.
c. Press up or down arrow button to choose the desired display mode (B&W, B&W Invert, Color1,
Color Invert1).
d. Press “OK” again to return to “Scan Mode”.
*The default setting of the scanner is Black & White in normal condition.
1: “Color/Color Invert” option only occurs when in white light application. 16

“Set Up Mode”
a. When icon displays on the top left corner of the screen, press “OK” to get into function
Menu option list.
b. Press right arrow button to choose “Set Up Mode”
C. Press down arrow button to get into “Setup Menu” and select the desired settings items.
d. Press right arrow button to get into settings mode. Then press up or down arrow button
to select the desired settings. Press “OK” to save the settings.
e. Press down arrow button to select other settings modes; or press “OK” again to return to
“Scan Mode”.
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“Set Up Mode”
Setup Menu and related setting items:
a. LCD: Dark, Normal, Bright
b. Display: LCD, VGA
c. Video Output: 800x600, 1024x768
d. Filename: Edit scanned file name (6 characters maximum)
e. Save Format: TIF, JPG, BMP (“JPG” is default setting)
f. Quick View: 1 sec, 2 sec, 3 sec, off (“2 sec” is default setting)
g. Slide Show Cycle: 2 sec, 3 sec, 5 sec, 10 sec (“3 sec” is default setting)
h. Power Save: 1 min, 3 min, 5 min, 10 min, off (“1 min” is default setting)
i. Language: English, Japanese
j. Date: setup date yyyy/mm/dd
k. Time: setup time hh/mm/ss
l. Reset: Yes, No. (If “YES”, reset all current settings back to factory default)
M. About: Unit Name, S/W version, F/W version, Update, CWD information

D. Function Menu Options II: Operation
“Play Mode”
a. When icon displays on top left corner of the screen, press “OK” to get into
functional options.
b. When “Scan Mode” icon is highlighted press “OK” again to get into “Play Mode”.
c. The “Play Mode” icon will display on top left corner of screen. The captured
images can then be reviewed one by one by pressing right arrow button.
d. Press “OK” twice to get back to “Scan Mode” (It will take few seconds to complete
the process.)
Move/Zoom Mode
e. Move/Zoom Mode: Zoom in/out captured images by pressing buttons.
Press up/down arrow button and left/right arrow button so the image can be
moved upward/downward and leftward/right.
Press to exit the Move/Zoom Mode.
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“Auto Play Mode”
a. When in “Play Mode”, press “OK” to get into functional option list.
b. Press right arrow button to choose “Auto Play Mode” then press “OK”.
c. The captured image will automatically display on LCD screen one by one.