Pacific Image Electronics Slite 600 User manual

Slite 600/600C
Gel Documentation SYSTEM
USER’S MANUAL

For over 15 years, Pacific Image Electronics Co., Ltd. has been continuously devoting tremendous
RD energy toward various fields such as electronic engineering, imaging analysis, and optics
application to develop and manufacture the most innovative electronic products that exactly
fulfill customers’ needs. Based on accumulated professional experiences for more than a
decade on developing the latest electronic products PIE has successfully crossed the gaps in
between electronics and biomedicine and farther marches forward to biomedical field with a
series of innovative lab instrument and reagent products that help researchers to approach their
goals in high level of biomedicine research. Further with foresightingly conceptual
combinations of product categories on lab required instruments and experimental reagents, PIE
has been accomplishing tasks on integration of instruments and reagents to provide quality
and innovative lab automatic instruments for
researchers to conduct research projects with high efficiency and high accuracy.
SAFETY INFORMATION
NOTE: All PIE UV Transilluminators are powerful sources of Ultraviolet (UV) radiation that can
cause damage to unprotected eyes and skin. Be sure all personnel in the area are properly
trained and protected before operating any unit that uses UV radiation.
It is further recommended that the transilluminator be installed and operated in a darkroom
where access and exposure is limited while the unit is in operation. For your safety and for the
safety of those around you, the transilluminator in an PIE darkroom is controlled through the
darkroom, and each darkroom is equipped with a safety switch that controls the power to the
transilluminator to prevent accidental exposure.
.
For Research Only
Pacific Image Electronics Co., Ltd. Web: www.scanace.com
TEL/[phone] FAX[phone]
7F, No. 239, Sec. 1 Datong Rd., Xizhi Dist., New Taipei City 221, Taiwan

2
INDEX
Introduction
3
System Components
4
Optional
Equipment 5
System Quick Set
-up 6
The Initial Configuration Settings Before Use
6
Slite
600 Quick Guide for Agarose EtBr Gel 7
Setting Manager
9
Slite
600 Operation Manual 10
Illuminator
Setting and Operation 13
WIFI
Setting and Control
16
T
roubleshooting 17
Hardware Specifications
18
Warranty
19
Technical Supports
19

INTRODUCTION
Slite 600 is a compact gel documentation system with user-friendly features of easy operation
and quick response. This imaging system consists of a scientific CCD camera, motor driven
lens, 595nm filter, UV transilluminator, and white backlight board. The chemi-fluorescence
stains such as EtBr, Cy2, SYPRO Ruby, SYBR Green I/II, SYBR Gold, etc...excited by UV can
easily be observed and captured by Slite 600. Followed the design concept of a single,
independent workstation on bench-top, Slite 600 does not require any desktops to work with.
A built-in touch screen LCD panel Slite 600 can directly handle the image data process
without any difficulty. Moreover, smart user friendly interfaces and vivid icons Slite 600 has
rapidly capture gel images with an easy manner. With having Slite 600 in the laboratory, you
will experience a hassle-free while working on the gel imaging acquisitions..
Power Button
USB Port
UV Transilluminator
LCD Touch Screen
Ethernet Port
USB Port X2
Main Power Switch
AC-in Jack
SYSTEM COMPONENTS
3

Camera CMOS
A camera equipped with high resolution 6M (3096 x 2080) CMOS sensor
Lens
F1.2, 8.5～51mm zoom lens
EtBr Filter
56mm, 595±45nm Filter
Darkroom
The darkroom is absolutely tightly sealed for optimizing
imaging conditions. The darkroom features:
•Overhead white light (Epi white light)
• Roll in-and-out UV transilluminator
White Backlight Board
Provide a bright surface to visualize Coomassie/silver stained
gels and colony plates. It also applies for the illumination of
samples with transilluminating white light.
UV Transilluminator
UV transilluminator allows the excitation of multiple fluorescent
dyes to occur. To protect users from UV exposure, UV light is
automatically shut off when door is open. UV transilluminator
can be slided in/out of cabinet smoothly.
LCD Touch Screen
A built-in 8-inch Multi-Touch LCD screen allows users to preview,
snap, save and print images, as well as to select certain preference
options without the connection of extra external keyboard or mouse.
4

Cyan/Red Epi illuminator
Cyan/Red Epi illuminator allows the excitation of several fluorescent dyes,
coming with high efficient light intensity for full excitation stained by both EtBr & its substitutes
(Safe dyes). It can be placed on top of UV transilluminator and be well accommodated with Slite
600.
Optional Accessories and Parts
Please contact your local PIE dealer for more information of the optional accessories and parts
Filters
·535nm/35: For gels or plates stained with SYBR Green, SYBR Safe, SYBR Gold, Fluorescein,
Green Fluorescent Protein stains.
Thermal Printer
The USB connected compact digital thermal printer may print out archive quality prints in
seconds (default: SONY D897 thermal printer)
5

SYSTEM SET UP
Please read Slite 600 manual
Follow Package Unpacking Guide to unpack the package before use.
The Initial Configuration Settings Before Use
After turning on the power of Slite 600 , the “IMAGE CAPTURE MENU” page will show on screen.
To enter “Settings” page you may press on “IMAGE CAPTURE MENU” page. The
parameter settings on “Tool” page may help you to obtain the desired image data. The parameters
such as “ Network”, “Image Size”, “Bit Depth”, “Save Format”, “Date” / “Time” and many other
settings can be reset more directly on screen
.
Image Capture Mode Settings
USB Flash Drive (FAT, FAT32, NTFS)
Please note the USB flash drive pre-formatted in the most popular FAT, FAT32, or NTFS type file
system is applied to Slite 600. All three USB ports on system are available for USB Flash Drive
installation for data storage.
Only one USB Flash Drive may
be recognized for data storage
when more than one USB Flash
Drive are Inserted.
USB Flash Drive Insertion
6

Slite 600 FOR AGAROSE ETBR GEL
✦Switch to power on the system by pressing the Main Power Switch located on the rear side
of the instrument.
✦Turn the system on by one-press of the Power Button on the front side of the instrument.
A “Beep” sound is heard.
✦The system will initiate the boot-up step.
✦When boot-up is completed, the LCD on Slite 600will display the IMAGE CAPTURE MENU page as
on Page 7.
✦Open the chamber door. (The automatic turn-on of Epi white light while door is open
makes users easy to place the sample into the cabinet. )
✦Slide out the UV transilluminator and place the staining gel onto UV transilluminator or light
board.
✦Slide the transilluminator back to the original position in chamber.
✦Press to enter SETTING MENU as on Page 7.
✦Select for basic settings.
✦Press back to IMAGE CAPTURE MENU.
✦Press to turn UV light on.
✦Press to adjust aperture.
✦Press to adjust zoom in or zoom out.
✦Press to adjust focus .
7

Slite 600 FOR AGAROSE ETBR GEL (CONTINUE)
✦Press to get into for No 1. filter selection , press for
ETBR gel observation.
✦Adjust exposure time, then press to capture image, Slite 600 screen will get into
IMAGE PERUSE MENU.
✦Press to convert the color into its complementary color.
✦Press to print image (Digital thermal printer must be connected.).
✦Press to save image to USB flash drive.
✦Press to delete the on-screen image, press to clear all images.
✦Press to back to IMAGE CAPTURE MENU.
IMAGE CAPTURE MENU
SETTING MENU IMAGE PERUSE MENU 8
1

SETTING MENU:
Network: Please refer to page 16
Bit Depth: Select 8-bit by pressing , select 16-bit by pressing .
Save Format: Choose desired format of image file by pressing TIF (8-bit, 16-bit), JPG (8-bit), and
BMP (8-bit) while saving the files.
About: Press to check the basic information of the system such as Machine ID, IP, Software
Version, Firmware Version,…etc. Press to resume all settings back to factory default value.
Others:
Info Print: Press to display the image info on the printout data.
Sleep: Press to activate the hibernating mode. Press screen to wake up hibernating
system.
Sat.: Press to activate “saturation display” function.
Date and Time: Press to set Date/time.
When settings are all finished, press , your settings are saved and the LCD screen will be back
to IMAGE CAPTURE MENU. You can start to capture the images.
SETTING MENU 9

Slite 600 OPERATION MANUAL
Slite 600 is an easy-operated gel documentation system. Users may easily capture gel images
through their fingertips by touching the functional icon showing on the built-in touch LCD screen.
User Interface Preview
Through functional icons showing on touch LCD screen, image data can be selected, adjusted,
saved, and printed with convenience.
IMAGE CAPTURE MENU:
Light Source: For all light sources, press the light source icon to turn on the light source; press
the same icon again to turn off the light source.
Press to turn on 312nm UV light.
Press to turn on Epi white light in the chamber.
Press to turn on white backlight.
(When white backlight board is installed, the white backlight board light source
selection icon will automatically display on screen.)
Press to turn on cyan light*
(When cyan light board is installed, the lighting source selection icon will be
available & displayed on screen.)
*Cyan Epi illuminator unit is only available on XXX model.
Press to adjust aperture value.
Press to adjust zoom in and zoom out for image size.
Press to adjust focus. Press below pull par to process
auto-focus function when needed. Auto-focus will be processed one round per
press.
10

Filter Lens Selection: Press to select the filter lens.
Press the icon to select the desired lens (Filter lens will always come to position 1
(595 nm) automatically when the light source changes)*.
*: Position 2 to 5 are empty and are available for further filter lens installation.
Capture Image: Press to capture image.
Exposure Time Setting: Press arrows on screen to select the desired
exposure time, the range is from 0.001 seconds ~ 10 seconds.
Captured Image Preview: Captured image will be temporarily stored in the system.
Press the image to display the captured image in IMAGE PERUSE MENU.
Users may further process the captured image by using the functional icons in the menu.
Press arrows to select the desired captured image.
Prefer: Press into SETTING MENU.
IMAGE CAPTURE MENU
11

IMAGE PERUSE MENU:
Press to convert the color into its complementary color.
Press to print image (Digital thermal printer must be connected.).
Press to save image to USB flash drive.
Press to delete the on-screen image, press to clear all images.
Press the image to display the captured image in IMAGE PERUSE MENU. Users may
further edit the captured image by using the functional icons in the menu.
Press arrows to select the desired captured image.
Change the file name of the captured image:
Press , a keyboard will show on the screen.
Users may delete the filename by pressing the
backspace key on the keyboard and rename the file by typing the desired letters..
Once the filename is retyped, press “OK” to complete the file rename work.
Press to back to IMAGE CAPTURE MENU.
Filename of the captured image
IMAGE PERUSE MENU 12

ILLUMINATOR SETTING AND OPERATION
Slite 600 is an easy-operated gel documentation system. Users may easily capture gel images
through their fingertips by touching the functional icon showing on the built-in touch LCD screen.
UV Transilluminator
Through functional icon showing on touch LCD screen,
UV transilluminator can be activated and the image data stained
by UV-excited fluorescent dye can be selected, adjusted, saved,
and printed with convenience.
White Backlight Board
White Backlight Board is designed for observing the colorimetric
dye stained gel data. The gel image data may also be obtained
through Slite 600.
Through functional icon showing on touch LCD screen,
White backlight board can be activated and the image data stained
By colorimetric dye can be selected, adjusted, saved, and printed with convenience.
Cyan/Red Epi illuminator
Cyan/Red Epi illuminator module is designed for observing the Cyan light-excited fluorescent dye-
stained gel data. The gel image data may also be obtained through Slite 600.
Through functional icon showing
on touch LCD screen, Cyan light source can be pre- activated and
the image data stained by either Cyan fluorescent dyes can be
selected, adjusted, saved, and printed with convenience.
Light Board Installation
1. Open the door of the chamber
2. Sliding the UV transilluminator out of the chamber
3. Place the light board on top of the UV transilluminator with
the pads of the light board fitting in the positioning holes on
the UV transilluminator.
4. Make sure the light board is immobilized.
(Continue next page)
Light board connector
13

5. Insert the connector of the light board into the inlet on the front
panel of UV transilluminator . The system will identify which light
board is inserted and will automatically show the light source icon
on the screen.
6. Operate the further image process work.
How to change the illuminator:
1. Turn system power off.
2. Slide out the illuminator from the chamber
3. Unplug the power cord (and connector, if any) on the right side of the back of the illuminator
4. Slide the illuminator out of the chamber to the end.
5. Find the plastic pad on the track underneath the illuminator, one plastic pad per track.
6. Hold the illuminator and press the plastic pad per hand and slightly slide the illuminator out of
the track.
7. For the new illuminator installation, find the plastic pads on the track underneath the new
illuminator. Hold the illuminator, press the plastic pads, and align the tracks of the illuminator
to push the illuminator gently into tracks. The illuminator is on the right track when a “click”
sound is heard.
8. Connect the power cord (and connector, if any).
9. Turn on the power once again.
Inlet for light board
14

15
Slite 600
UV Lamps Exchange Manual
1. Open device door and pull out UV box. Use screwdriver to remove side screw (as circled).
2. Slowly lift the enclosure cover and toward approximately 5cm to take it off.
3. Gently rotate UV lamps by hands approximately 90 degree till logo imprints and
connector pins can be seen below to take off the lamps, then exchange UV replacement(s)
accordingly.

WIFI SETTING AND CONTROL
Slite 200W series has a build-in wireless access point ( AP ) and is controled by web browser
interface (IE, Chrome, Safari, firefox, etc.). After turning on the power, user can connect Slite
200W series wirelessly with PC, Mac, tablet, and smartphone which have wifi connection ability
and web browsers. The interface and the controls are the same as Slite 200W standalone
interface (page 7).
How to connect Slite 600 to your wireless device through WIFI
(PC, Notebook, tablets, smartphone)
1. Open the internet wireless connection in the control panel of your wireless
process ends.
2. Find “MyWLAN”and connect.
3. From your wireless process end, open a web browser. On the webpage address
site type in 192.168.1.66 then press enter.
4. A dialogue for log on the connection is shown..
Login name: admin
Password: 0000
Press Ok to log in.
For guest login,
Login name: admin
Password: 0000
Maximum 5 people are allowed for guest login.
5. You will see the main operation page of
Slite 200W shown on your wireless process end.
6. You may thus start to operate the system.
16

No
Power to the
Darkroom
Check the connection of main power cord to
main power port located on the rear side of
Slite
600.
Check fuse; the fuse cassette is located on the
rear side beside the power port. You will need
a flatted
-head screwdriver to lever the fuse
cassette out of the insertion site. In the fuse
cassette, you may find a spare fuse underneath
the working fuse. Observe the working fuse to
see if there is any break in the wire inside the
glass tube. If so, replace the torn
fusen with
the spare one. If fuses are consistently blown,
contact you local PIE dealer.
T
ransilluminator Will
Not
Turn On
There is a power cord connecting
Slite 600
with UV
transilluminator. Check to see if
the power cord is loose by sliding UV
transilluminator
out of cabinet to the end.
If so, tightly plug the power cord back to
UV
transilluminator.
If still not turning on, unplug the power cord
and replace with other power cord, hook up
replaced power cord to UV
transilluminator
and
plug into the power socket anywhere you can
find except the ones in
Slite 600. If the UV
transilluminator
lights on, it represents that
there may have problem on electric circuits
inside of
Slite 600. Contact your local PIE
distributor for further repair process. If still not
on, it represents the UV
transilluminator is out
of order. Also contact your local PIE distributor
for further assistance
.
TROUBLESHOOTINGS
17

HARDWARE SPECIFICATIONS
Image
Capturing Unit
CMOS
Sensor
6M
CMOS Monocolor
Pixel
Size (µm, VxH)
2.45
x 2.45
Exposu
re Time
Up
to 10 sec
Cooling
Temperature
Room
Temperature
Captu
re Resolution
High:
2927 x 2054
Standard:
1463 x 1027
Bit
Depth
14 bit
PC
Interface
None
Lens
Type
6 X
Zoom Lens
Powered Lens
V
Auto
Focus
V
Filter
Set
Filter
Turret
Moto
r-Driven, 6 position
Emission
Filter (One
standa
rd set; Five for
optional
order)
595 nm
(EtBr Stain)
Cont
rol Set
Display Panel
Build
-in 8” Touch TFT LCD
Standalone Cont
rol
T
ouch Screen Interface
PC
Control Interface
None
Image
Storage
USB
Storage & Wi-Fi
Lighting
Set
UV
Transillumintor (20cm X
20cm)
V
White Back
Light
V
Epi
White Light
V
Cyan/Red illuminator
Optional
General
Info
Power
100
V-240V
Dimension (WxHxD)
303x450x600mm
W
eight
30Kg
Certification
FCC,CE
18

Slite 600 Gel Documentation System
Input：AC100-240V
50/60Hz
200W
Pacific Image Electronics Co., Ltd. Web: www.scanace.com
TEL/[phone] FAX[phone]
7F, No. 239, Sec. 1 Datong Rd., Xizhi Dist., New Taipei City 221,
WARRANTY
The warranty of PIE Corporation shall be of no force and effects if buyer has modified or damage
the product.
TECHNICAL SUPPORTS
Please contact your local PIE distributor for further assistance & technical support in needed.