Phcbi MDF-U5412 User manual

MDF-U5412
Please read the operating instructions carefully before using this product, and keep the operating
instructions for future use.
See page 26 for model number.
Operating Instructions
Biomedical Freezer

1
CONTENTS
INTRODUCTION P. 2
PRECAUTIONS FOR SAFE OPERATION P. 3
ENVIRONMENTAL CONDITIONS P. 7
FREEZER COMPONENTS P. 8
Control panel P.10
INSTALLATION SITE P.11
INSTALLATION P.12
START-UP OF UNIT P.13
TEMPERATURE SETTING P.14
Key lock function P.14
ALARM TEMPERATURE SETTING P.15
SETTING OF ALARM RESUME TIME P.16
DURING/AFTER POWER FAITURE P.17
REMOTE ALARM TERMINAL P.18
ALARMS & SAFETY FUNCTIONS P.19
ROUTINE MAINTENANCE P.20
Cleaning of cabinet P.20
Defrosting P.20
REPLACEMENT OF BATTERY P.21
TROUBLESHOOTING P.22
DISPOSAL OF UNIT P.23
Recycle of battery P.23
Decontamination of unit P.23
TEMPERATURE RECORDER (OPTION) P.24
SPECIFICATIONS P.25
PERFORMANCE P.26
SAFETY CHECK SHEET P.27

2
INTRODUCTION
■Read the operating instructions carefully before using the product and follow the instructions for safe
operation.
■PHC Corporation takes no responsibility for safety if the product is not used as intended or is used with
any procedures other than those given in the operating instructions.
■Keep the operating instructions in a suitable place so that they can be referred to as necessary.
■The operating instructions are subject to change without notice for improvement of performance or
function.
■Contact our sales representative or agent if any page of the operating instructions is lost or the page
order is incorrect, or if the instructions are unclear or inaccurate.
■No part of the operating instructions may be reproduced in any form without the express written
permission of PHC Corporation.
IMPORTANT NOTICE
PHC Corporation guarantees this product under certain warranty conditions. However, please note that
PHC Corporation shall not be responsible for any loss or damage to the contents of the product.

3
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with the operating instructions
as it contains important safety advice.
Items and procedures are described so that you can use this unit correctly and safely.
If the precautions advised are followed, this will prevent possible injury to the user and
any other person.
Precautions are illustrated in the following way:
WARNING
Failure to observe WARNING signs could result in a hazard to personnel
possibly resulting in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.
Symbol shows;
This symbol means caution.
This symbol means an action is prohibited.
This symbol means an instruction must be followed.
Be sure to keep the operating instructions in a place accessible to users of this unit.
Some warning and/or caution labels are attached on the unit. Following shows the description of such
labels
This label is on the cover in which the electrical components of high voltage are
enclosed to prevent the electric shock. The cover should be removed by a qualified
engineer or service personnel only.
This symbol means attention or refer to document.
This symbol means earth.
This symbol means power switch “ON”.
This symbol means power switch “OFF”.

4
PRECAUTIONS FOR SAFE OPERATION
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to
rain water.
Only qualified engineers or service personnel should install the unit. The installation by
unqualified personnel may cause electric shock or fire.
Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from
turning over. If the floor is not strong enough or the installation site is not adequate, this may result
in injury from the unit falling or tipping over.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
Never install the unit in a flammable or volatile location. This may cause explosion or fire.
Never install the unit where acid or corrosive gases are present as current leakage or electric
shock may result due to corrosion.
Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not
grounded, it will be necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such
grounding may cause electric shock in the case of an incomplete circuit.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use of
any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit if the container cannot be sealed. These
may cause explosion or fire.
Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.
This may cause electric shock or injury by accidental contact with moving parts.
Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use
may cause bad effect on your health or environment.
Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any
repair or maintenance of the unit in order to prevent electric shock or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.
WARNING

5
PRECAUTIONS FOR SAFE OPERATION
Ensure you do not inhale or consume medication or aerosols from around the unit at the time of
maintenance. These may be harmful to your health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put containers with liquid on the unit as this may cause electric shock or short circuit when
the liquid is spilled.
Never bind, process, or step on the power supply cord, or never damage or break the power
supply plug. A broken power supply cord or plug may cause fire or electric shock.
Do not use the power supply cord if its plug is loose. Such power supply cord may cause fire or
electric shock.
Never disassemble, repair, or modify the unit yourself. Any such work carried out by an
unauthorized person may result in fire, or electric shock or injury due to a malfunction.
Disconnect the power supply plug if there is something wrong with the unit. Continued
abnormal operation may cause electric shock or fire.
When removing the power supply plug from the power supply outlet, grip the power supply
plug, not the cord. Pulling the power supply cord may result in electric shock or fire by short circuit.
Disconnect the power supply plug before moving the unit. Take care not to damage the power
supply cord. A damaged power supply cord may cause electric shock or fire.
Disconnect the power supply plug when the unit is not used for long periods. Keeping the
connection may cause electric shock, current leakage, or fire due to the deterioration of insulation.
If the unit is to be stored unused in an unsupervised area for an extended period, ensure that children
do not have access and that doors cannot be closed completely.
The disposal of the unit should be accomplished by appropriate personnel. Remove doors to
prevent accidents such as suffocation.
Do not put the packing plastic bag within reach of children as suffocation may result.
Do not position this unit and the other unit so that it is difficult to operate the disconnection of
the power supply plug. Failure to disconnect the power supply plug may cause fire if there is
something wrong with the unit.
WARNING

6
PRECAUTIONS FOR SAFE OPERATION
This unit must be plugged into a dedicated circuit protected by branch circuit breaker.
Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap
may cause fire resulting from abnormal heating.
Connect the power supply plug to the power source firmly after removing the dust on the plug.
A dusty plug or improper insertion may cause a heat or ignition.
Never store corrosive substances such as acid or alkali in this unit if the container cannot be
sealed. These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet when you request any repair or maintenance for the safety of service
personnel.
CAUTION

7
ENVIRONMENTAL CONDITIONS
This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):
■Indoor use;
■Altitude up to 2000 m;
■Ambient temperature 5 oC to 40 oC;
■Maximum relative humidity 80 % for temperature up to 31 oC decreasing linearly to 50 % relative
humidity at 40 oC;
■Mains supply voltage fluctuations up to ±10 % of the nominal voltage;
■Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;
■Temporary OVERVOLTAGES occurring on the mains supply;
■Applicable pollution degree of the intended environment (POLUTION DEGREE 2 in most cases)

8
FREEZER COMPONENTS
12
Back side
11
14
15
13
1
9
2
8
3
4
7
5
6
10
Space for interface board

9
FREEZER COMPONENTS
1. Outer door: To open the door, grip the handle. When closing, lock the door latch completely.
2. Handle: Always grip the handle to open the outer door.
3. Lock: Turn counterclockwise to 180 owith a key and the outer door is securely locked.
4. Defrost water vessel & storage container: The defrost water vessel & storage container can be
used to collect the defrosted water when defrosting.
Note: Do not pull the storage container excessively. The storage container may fall down.
5. Space for temperature recorder: A temperature recorder (optional component) can be attached here.
See page 24 “Temperature recorder”.
6. Leveling foot: These are screw bolts used to install and fix the unit. Adjust the height of the leveling
feet by turning the screw bolts until 2 front casters are away from the floor. See page 12
“INSTALLATION”.
7. Caster: 4 casters are provided to facilitate moving of the cabinet. For the installation, adjust the
leveling feet so that the front 2 casters cannot contact with the floor.
8. Door latch: To lock the outer door, turn this latch downward. A padlock is also available.
9. Storage container: Made of styrol resin. Be careful not to damage the storage container by a
scraper at the time of defrosting.
Note: Do not pull the storage container excessively. The storage container may fall down.
10. Control panel: To display the temperature setting and running condition. See page 10 for details.
11. Remote alarm terminal: Used to notify an alarm condition of the unit to remote location. See page
18 for details.
12. Battery switch: Switch for battery used for power failure alarm. Always keep “ON”. Turn the
switch “OFF” when the unit is in no use for a long period (more than 1 month).
13. Fixture (on back side): 2 fixtures are provided as spacers between the cabinet and wall and also
serve as hooks to fix the unit. See page 12 “INSTALLATION”.
14. Power switch: This is for turning ON/OFF the power to the unit. This switch also activates as an
over-current breaker (15 A).
15. Access port: This is used for leading a cable and sensor of a measuring equipment.
Note: The outer door does not open quickly because the inside of the chamber becomes negative
pressure after the outer door is closed.

10
FREEZER COMPONENTS
Control panel
1. Alarm lamp (ALARM): This lamp is flashed when the audible alarm is activated.
2. Digital temperature indicator: Normally, the current chamber temperature is displayed in the
temperature display. When the high/low temperature alarm is activated, the current chamber temperature
blinks. When the self-diagnostic function detects an abnormality, the error cord is displayed . Refer to
page 19.
3. Numerical value shift key ( ): Pressing this key in the setting mode causes the numerical value to
shift. ON-OFF of key lock can be selected by pressing this key in the key lock setting mode. By
pressing this key for more than 5 seconds in the temperature display mode leads setting mode for alarm
temperature, alarm resume time and compressor delay time . Refer to page 14,15 and 16 for details
respectively.
4. Digit shift key ( ): Pressing this key in the setting mode causes the changeable digit to shift. key
lock setting mode is led by pressing this key for more than 5 seconds in the temperature display mode.
Refer to page 14 for the key lock.
5. Set key (SET): At “temperature display mode” pressing this key leads “chamber temperature setting
mode”. At “setting mode”; stores the value into computer memory.
6. Defrost key (DEF): When removing the frost, press this key for 5 seconds. The freezer operation is
stopped. After removing the frost, press this key again. See page 20.
Note: The freezing operation never resumes automatically after defrosting.
7. Buzzer stop key (BUZZER): To silence the audible alarm, press this key. The remote alarm is also
stopped by pressing this key.
8. Alarm test key (ALARM TEST): Test key for alarm device. By pressing this key, the alarm lamp is
flashed, remote alarm is activated and buzzer sounds. This means all alarm function operate correctly.
This key is available only during normal running.
8 7 6 5 4 3
1 2

11
INSTALLATION SITE
To operate this unit properly and to obtain maximum performance, install the unit in a location with the
following conditions:
■A location not subjected to direct sunlight
Do not install the unit under direct sunlight. Installation in a location subjected to direct sunlight cannot
obtain the intended performance.
■A location with adequate ventilation
Leave at least 10 cm around the unit for ventilation. Poor ventilation will result in a reduction of the
performance and consequently the failure.
■A location away from heat generating sources
Avoid installing the unit near heat-emitting appliances such as a heater or a boiler etc. Heat can
decrease the intended performance of the unit.
■A location with little temperature change
Install the unit under stable ambient temperature. The allowable ambient temperature is between +5 oC
and +30 oC.
■A location with a sturdy and level floor
Always install the unit on a sturdy and level floor. The uneven floor or tilted installation may cause failure
or injury. Install the unit in stable condition to avoid the vibration or noise. Unstable condition may
cause vibration or noise.
WARNING
Install the unit on a sturdy floor. If the floor is not strong enough or the installation site is not adequate,
this may result in injury from the unit falling or tipping over.
Select a level and sturdy floor for installation. This precaution will prevent the unit from tipping.
Improper installation may result in water spillage or injury from the unit tipping over.
■A location not prone to high humidity
Install the unit in the ambient of 80 %R.H. or less humidity. Installation under high humidity may cause
current leakage or electric shock.
WARNING
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to rain
water.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
■A location without flammable or corrosive gas
Never install the unit in a location where it will be exposed to flammable or corrosive gas. This may cause
explosion or fire or may result in the current leakage or electric shock by the corrosion of the electrical
components.
■A location without the possibility of anything fall
Avoid installing the unit in the location where anything can fall down onto the unit. This may cause the
breakdown or failure of the unit.

12
INSTALLATION
1. Removing the packaging materials and tapes
Remove all transportation packaging materials and tapes. Open the outer doors and ventilate the unit.
If the outside panels are dirty, clean them with a diluted neutral dishwashing detergent. (Undiluted
detergent can damage the plastic components. For the dilution, refer to the instruction of the detergent.)
After the cleaning with the diluted detergent, always wipe it off with a wet cloth. Then wipe off the panels
with a dry cloth.
Note:
Remove the cable tie banding the power supply cord.
Prolonged banding may cause the corrosion of the cord
coating.
2. Adjust the leveling feet
Stretch the leveling feet by rotating them to contact them to
the floor. Ensure the unit is installed horizontally. (Fig.1)
3. Fixing the unit
Two fixtures are attached to the rear of the frame. Fix the
frame to the wall with these fixtures and rope or chain.
(Fig. 2)
4. Ground (earth)
The ground (earth) is for preventing the electric shock in the case of unexpected deterioration of the
electrical insulation. Always ground the unit at the time of installation.
WARNING
Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet is
not grounded, it is necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such
grounding may cause electric shock in the case of an incomplete circuit.
Fixture Fig. 2
Fig.1
Stretch
Leveling foot

13
START-UP OF UNIT
Use the following procedure to start trial operation or actual operation of the unit.
1. Connect the power supply cord to the dedicated outlet having appropriate rating with the chamber
empty, and turn on the power switch on the biomedical freezer.
2. Turn on the battery switch.
3. Set the desired chamber temperature. See page 14 for the temperature setting.
4. Check that the chamber temperature reaches the desired temperature.
5. Make sure that the alarm lamp flashes and the buzzer sounds by pressing the alarm test key (ALARM
TEST). The remote alarm is also operated. E09 is displayed on the control panel and buzzer sounds if
the battery switch is off. Make sure to turn on the battery switch.
6. After confirming the above, you can put articles into the biomedical freezer chamber in a small batch to
prevent the temperature rise.
Note:
■When starting the operation of the biomedical freezer for the first time, the alarm lamp (ALARM) lights
after the start of operation. When the chamber temperature reaches around the set temperature, then
the alarm lamp goes out (The remote alarm is not activated).
■If the battery switch is turned on before turning on the power of the biomedical freezer, the temperature
alarm is activated and the buzzer sounds and the remote alarm is also activated after the start of operation.
Check that the battery switch is off before turning on the biomedical freezer.

14
TEMPERATURE SETTING
Table 1 shows the basic procedure for setting the chamber temperature. Perform key operations in the
sequence indicated in the table. The example in the table is based on the assumption that the desired
temperature is -25 oC.
Note: The chamber temperature is set to -40 oC at the factory.
Table 1. Basic operation sequence (Example: Chamber temperature -25
oC)
Description of operation Key operated Indication after operation
1 Turn on the power switch. ---- The current chamber temperature is
displayed.
2 Press set key. SET The second digit is flashed.
3 Set to -25 with the numerical value
shift key and digit shift key.
When pressed, the figure of settable
digit changes.
When pressed, the settable digit
is shifted.
4 Press set key. SET
Set temperature is memorized and the
current chamber temperature is
displayed.
Note:
• Although the value of the chamber temperature setting can range from -18 oC to -45 oC, the guaranteed
temperature with no load is -40 oC when the ambient temperature is 30 oC.
• The setting mode returns to the temperature display mode automatically when 90 seconds has passed
without any key operation. In this case, any setting before pressing set key (SET) is not memorized.
Key lock function
This unit is provided with the key lock function. When the key lock is ON, change of temperature setting
through the key pad is not possible. The key lock is set to OFF at the factory.
Display Mode Function
L 0 Key lock is OFF Enable to change temperature setting
L 1 Key lock is ON Disable to change temperature setting
Table 2. Procedure for key lock setting (change from key lock OFF to key lock ON)
Description of operation Key operated Indication after operation
1 ---- The current chamber temperature is
displayed.
2 Press digit shift key for 5 seconds.
The first digit is flashed.
3 Press numerical value shift key
and scroll the figure to 1. When pressed, the figure of settable digit changes.
4 Press set key. SET
The key lock is set to on.
The current chamber temperature is
displayed.
• The setting mode returns to the temperature display mode automatically when 90 seconds has passed
without any key operation. In this case, any setting before pressing set key (SET) is not memorized.

15
ALARM TEMPERATURE SETTING
This unit is provided with both high and low temperature alarms. The temperature at which the alarm is
activated can be changed.
The available set range for high temperature alarm is between +5 oC and +15 oC, and -5 oC and -15 oC for
low temperature alarm against the set temperature.
Note: The temperature alarm is set at ±10 oC of the set temperature at the factory.
As an example, table 3 shows the procedure to set the high temperature alarm so that the alarm can
activate when the chamber temperature is 5 oC higher than the set temperature.
Table 4 shows the procedure to set the low temperature alarm so that the alarm can activate when the
chamber temperature is 5 oC lower than the set temperature.
Table 3. Procedure for setting high temperature alarm
Description of operation Key operated Indication after operation
1 ---- The current chamber temperature is
displayed.
2 Press numerical value shift key for
about 5 seconds. The first digit is flashed.
3 Press numerical value shift key
and scroll the figure to 1. When pressed, the figure of settable digit changes.
4 Press set key. SET The current setting is displayed and
the first digit is flashed.
5
Scroll the figure to 005 by using
digit shift key and numerical value
shift key
When pressed, the figure of settable
digit changes.
When pressed, the figure of
settable digit is shifted.
6 Press set key. SET
Alarm temperature is memorized and
the current chamber temperature is
displayed.
Table 4. Procedure for setting low temperature alarm
Description of operation Key operated Indication after operation
1 ---- The current chamber temperature is
displayed.
2 Press numerical value shift key for
about 5 seconds. The first digit is flashed.
3 Press numerical value shift key
and scroll the figure to 2. When pressed, the figure of settable digit changes.
4 Press set key. SET The current setting is displayed and
the first digit is flashed.
5
Scroll the figure to -05 by using
digit shift key and numerical value
shift key
When pressed, the figure of settable
digit changes.
When pressed, the figure of settable
digit is shifted.
6 Press set key. SET
Alarm temperature is memorized and
the current chamber temperature is
displayed.
• The setting mode returns to the temperature display mode automatically when 90 seconds has passed
without any key operation. In this case, any setting before pressing set key (SET) is not memorized.

16
SETTING OF ALARM RESUME TIME
The buzzer and remote alarm are silenced by pressing buzzer stop key (BUZZER) on the control panel
during alarm condition. However, if the alarm condition is continued after the “alarm resume time” has
passed, the buzzer sounds again.
It is possible to change the alarm resume time by the procedure shown in the Table.5.
The example in the table is based on the assumption that the desired duration is 20 minutes.
Note: Factory setting; 30 minutes.
Table 5. Setting procedure for alarm resume time (change from 30 minutes to 20 minutes)
Description of operation Key operated Indication after operation
1 ---- The current chamber temperature is
displayed.
2 Press numerical value shift key for
5 seconds. The first digit is flashed.
3
Set the figure to F25 with the digit
shift key and numerical value shift
key.
When pressed, the settable digit
is shifted.
When pressed, the figure of settable
digit changes.
4 Press set key. SET The current reset time is displayed.
The second digit is flashed.
5 Scroll the figure to 020 by using
numerical value shift key.
When pressed, the figure of settable
digit changes.
6 Press set key. SET
Alarm resume time is memorized and
the current chamber temperature is
displayed.
• The settable alarm resume time are 10, 20, 30, 40, 50, or 60 minutes. The buzzer and remote alarm
would not resume if the resume time is set in 000.
• The buzzer and remote alarm during power failure or alarm testing cannot be stopped.
• The setting mode returns to the temperature display mode automatically when 90 seconds has passed
without any key operation. In this case, any setting before pressing set key (SET) is not memorized.

17
DURING/AFTER POWER FALURE
■Display of chamber temperature during power failure
By pressing the buzzer stop key (BUZZER) during “power failure alarm”, the buzzer stop and the chamber
temperature is displayed on the temperature display for 5 seconds.
Check the chamber temperature as appropriate by pressing the buzzer stop key (BUZZER) during
power failure.
<Important>
The battery for power failure alarm is a consumable part. Replace the battery about every 3 years. The
alarm would not be activated at power failure if the battery is not replaced regularly. Contact our sales
representative or agent for the replacement of battery.
■Operation check after recovery from the power failure
The set value is memorized by nonvolatile memory. Accordingly, the freezer resumes the operation with
setting before power failure.
When the freezer is recovered from power failure with the chamber temperature higher than the preset
temperature, then the high temperature alarm is activated and the buzzer sounds and the remote alarm is
also activated. Please push the buzzer stop key (BUZZER) to silence buzzer and take appropriate
actions if needed.

18
REMOTE ALARM TERMINAL
WARNING
Always disconnect the power supply cord before connecting an alarm device to the remote alarm
terminal.
The remote alarm terminal is installed at the back of the unit. The alarm is outputted from this terminal.
Contact capacity is DC 30 V, 2 A.
Contact output:
Contact output:
Terminal Normal status Abnormal status
Between COM. and N.O. open close
Between COM. and N.C. close open
Note:
• The remote alarm is stopped by pressing buzzer stop key (BUZZER) as the remote alarm is operated in
conjunction with the buzzer, except for the power failure alarm.
• The remote alarm terminal is in alarm status when the power supply plug is unplugged because it is
regarded as a power failure.